pycom961g00010

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00000961
OG0002208
Physical Location & Seq
Forward (+)
18314 .. 21886
3573 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom961g00010.6

Sequence Viewer

Length: 2076 bp
ATGCTATATAGGGGGGTGGTGCTCCATGCATTTCAAGCAGCCTGCACCATCGCTCTAGGGATGACATTCTCGACCATCATCACGTGTTGCCTCGGTATATATGATGTGTCGGGTTTGGGACATTCCCGCCCATGGTGCCGACGACGGTGGCGGTCACCCCCGCCACCGCCGCCGACCCCCACCACCATGGGTGCGTTTTCGTTTTTTTTTTTTTTTTTCGAAAAAAAAAAAAATTTGGTGGGATCGGCTTTGGATGAACCAATGCACAGGCTTGTCCTAGGCATGTTAGCGGGTGCACGGCAATGCAGTCCATGGACGTTTGTTTTCCGAAGGGCTCCGGTGCGGCTCGGCTCCTACCTGGCGTATTTGCACTCAAGACGCATCCCTTTACGGGGCTGGTCGAGACGTGTTTTTCGTCGGGCGGTGCTGGGTTTAGCTACGCGACGGCGTATGAGTGGTAATTGGGTTGTATTCGACGGCAGGCTCCGTGCTTCGGCATCGAACTGTCGGCTCGTACCCCGCTTCATCGACGCGTCGAGATTCGTCTCGTGCGCAGGATGGGTTTCTGTTTTGGCTACCTGCTGCGAAGGAACGTTGCCTCTCGTCGTCCCTTTCCTCCTCCTGCCGGCCTCGCCGGCGGGAGGACGACATCGTGGCGGTGCTCGTGTCCCGGACGAGCCGCACTCGTTGCGGGGCGGTGGGTCTTCACGACCTCCGATCGTCCGATCGAAGCCTTGTCGCTCGACGCGTACGACCGTCGCGCCCGCTGCGAGCCGTCGACCGTCAAGGTCGTCCGTCTCGCGCGACGCCGACGTCGAAGAGGAATGCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATTTATAGTTTGTTTGATGGTATCTGCTACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAACAAACCCCGACTTCTGGAAGGGACGCATTTATTAGATAAAAGGTCGACGCGGGCTCTGCCCGTTGCTCTGATGATTCATGATAACTCGACGGATCGCACAGCCATCGTGCTGGCGACGCATCATTCAAATATCTCTCGTAGTTGGACCTTGGGTTGGGTCGACCGGTCCGCCTCTGGTGTGCACCGGTCGGCTCGTCCCTTCTGCCGGCGATACGCTCCTGGCCTTAATTGGCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGATTTCGGTCCTATTCTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCGACGAACAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGGCGGATGTTACTTTTAGGACTCCGCCGGCACCTTATGAGAAATCAAAGTTTTTGGGTTCCGGGGGAGTATGGTCGCAAGTGGAGCCTGCGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGGTCATCCTCCGCGGCCAGCTTCTTAGAGGGACTATGGCCATCATTGCAATTGTTGGTCTTCAACGAGGAATTCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTCCTACCGATTGAATGGTCCGGTGAAGTGTTCGGATCGCGGCGACGCGGGTGGTTCGCTGCCCGCGACGTCGCGAGAAGTCCACTGAACCTTATCATTGATCATTGTCGAACCTGCGCAGCAGAACGACCCGAGAACCCGTTCCCACGTCGGGGGCCGGCGGGCCTTCGGGCTCGGCGTCCCCTTATCCCGGGAGCCCGCTCCCGGGCGTACCAACGAACACCGGCGCGTCTTTGCGCCAAGGAACCCGAACGAAAGAGCGCGCTCCCGCCGCCCCGGAAACGGTGCGCGCGCGGGCGGCGTCGTCGTCTTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

692

Amino Acids

76.5

Weight (kDa)

11.12

Isoelectric Point (pI)

65.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 812
AatII GACGTC 2 cut(s) 816, 1830
Acc16I TGCGCA 2 cut(s) 553, 1876
Acc36I ACCTGC 2 cut(s) 587, 1880
AccB1I GGYRCC 2 cut(s) 135, 1444
AccBSI CCGCTC 1 cut(s) 1959
AccI GTMKAC 4 cut(s) 778, 995, 1110, 1294
AclI AACGTT 1 cut(s) 593
AclWI GGATC 6 cut(s) 250, 833, 1050, 1274, 1420, 1801
AcoI YGGCCR 4 cut(s) 1181, 1538, 1633, 1656
AcsI RAATTY 2 cut(s) 232, 1689
AcvI CACGTG 1 cut(s) 84
AcyI GRCGYC 5 cut(s) 807, 813, 1827, 1936, 2059
AfaI GTAC 4 cut(s) 516, 751, 1744, 1970
AflIII ACRYGT 4 cut(s) 83, 406, 531, 746
AgeI ACCGGT 2 cut(s) 1113, 1134
AgsI TTSAA 5 cut(s) 35, 1077, 1213, 1682, 1772
AjiI CACGTC 2 cut(s) 407, 1907
AjnI CCWGG 4 cut(s) 357, 831, 1168, 1407
AjuI GAANNNNNNNTTGG 2 cut(s) 1313, 1345
AluBI AGCT 5 cut(s) 437, 938, 1611, 1639, 1715
AluI AGCT 5 cut(s) 437, 938, 1611, 1639, 1715
Alw21I GWGCWC 5 cut(s) 24, 298, 664, 1134, 1229
Alw26I GTCTC 6 cut(s) 397, 550, 802, 868, 1390, 1584
Alw44I GTGCAC 2 cut(s) 294, 1130
AlwI GGATC 6 cut(s) 250, 833, 1050, 1274, 1420, 1801
Ama87I CYCGRG 3 cut(s) 1889, 1948, 1962
AoxI GGCC 9 cut(s) 627, 1171, 1181, 1257, 1538, 1633, 1656, 1913, 1921
ApaLI GTGCAC 2 cut(s) 294, 1130
ApeKI GCWGC 5 cut(s) 38, 582, 767, 1817, 1877
ApoI RAATTY 2 cut(s) 232, 1689
ArsI GACNNNNNNTTYG 2 cut(s) 396, 428
AseI ATTAAT 1 cut(s) 1335
AsiGI ACCGGT 2 cut(s) 1113, 1134
Asp700I GAANNNNTTC 1 cut(s) 1898
AspA2I CCTAGG 1 cut(s) 277
AspS9I GGNCC 6 cut(s) 1095, 1116, 1244, 1776, 1913, 1921
AsuC2I CCSGG 8 cut(s) 671, 1185, 1477, 1949, 1950, 1963, 1964, 2035
AsuHPI GGTGA 2 cut(s) 147, 1793
AsuII TTCGAA 1 cut(s) 219
AvaI CYCGRG 3 cut(s) 1889, 1948, 1962
AvaII GGWCC 4 cut(s) 1095, 1116, 1244, 1776
AvrII CCTAGG 1 cut(s) 277
BaeGI GKGCMC 2 cut(s) 298, 1134
BalI TGGCCA 1 cut(s) 1658
BanI GGYRCC 2 cut(s) 135, 1444
BanII GRGCYC 5 cut(s) 337, 1006, 1360, 1933, 1957
BauI CACGAG 2 cut(s) 547, 663
BbrPI CACGTG 1 cut(s) 84
BbsI GAAGAC 4 cut(s) 696, 1369, 1670, 2060
Bbv12I GWGCWC 5 cut(s) 24, 298, 664, 1134, 1229
BbvCI CCTCAGC 1 cut(s) 1595
BbvI GCAGC 5 cut(s) 50, 569, 754, 1804, 1889
BccI CCATC 6 cut(s) 56, 83, 552, 893, 1061, 1667
BceAI ACGGC 5 cut(s) 314, 461, 493, 759, 1525
BcgI CGANNNNNNTGC 2 cut(s) 1036, 1070
BciT130I CCWGG 4 cut(s) 359, 833, 1170, 1409
BclI TGATCA 1 cut(s) 1858
BcnI CCSGG 8 cut(s) 671, 1185, 1477, 1949, 1950, 1963, 1964, 2035
BcoDI GTCTC 6 cut(s) 397, 550, 802, 868, 1390, 1584
BfaI CTAG 6 cut(s) 56, 278, 933, 1382, 1608, 1695
BfoI RGCGCY 1 cut(s) 1203
BfuAI ACCTGC 2 cut(s) 587, 1880
BglI GCCNNNNNGGC 1 cut(s) 635
BlnI CCTAGG 1 cut(s) 277
Bme18I GGWCC 4 cut(s) 1095, 1116, 1244, 1776
BmeT110I CYCGRG 3 cut(s) 1889, 1948, 1962
BmgBI CACGTC 2 cut(s) 407, 1907
BmgT120I GGNCC 6 cut(s) 1095, 1116, 1244, 1776, 1913, 1921
BmsI GCATC 4 cut(s) 390, 506, 1078, 1390
BpiI GAAGAC 4 cut(s) 696, 1369, 1670, 2060
BplI GAGNNNNNCTC 2 cut(s) 668, 700
Bpu10I CCTNAGC 1 cut(s) 1595
Bpu14I TTCGAA 1 cut(s) 219
BpuEI CTTGAG 1 cut(s) 358
BpuMI CCSGG 8 cut(s) 671, 1185, 1477, 1949, 1950, 1963, 1964, 2035
BsaAI YACGTR 2 cut(s) 84, 945
BsaHI GRCGYC 5 cut(s) 807, 813, 1827, 1936, 2059
BsaI GGTCTC 1 cut(s) 1584
BsaWI WCCGGW 4 cut(s) 337, 1113, 1134, 1778
Bse118I RCCGGY 8 cut(s) 625, 634, 1113, 1134, 1155, 1441, 1915, 1981
Bse1I ACTGG 1 cut(s) 846
Bse3DI GCAATG 2 cut(s) 308, 1662
BseBI CCWGG 4 cut(s) 359, 833, 1170, 1409
BseGI GGATG 7 cut(s) 66, 259, 381, 563, 1330, 1426, 1623
BseMI GCAATG 2 cut(s) 308, 1662
BseMII CTCAG 1 cut(s) 1609
BseNI ACTGG 1 cut(s) 846
BsePI GCGCGC 3 cut(s) 2019, 2046, 2048
BseRI GAGGAG 1 cut(s) 608
BseSI GKGCMC 2 cut(s) 298, 1134
BseXI GCAGC 5 cut(s) 50, 569, 754, 1804, 1889
BseYI CCCAGC 1 cut(s) 427
BsgI GTGCAG 1 cut(s) 28
Bsh1285I CGRYCG 6 cut(s) 720, 728, 756, 782, 1114, 1139
BshFI GGCC 9 cut(s) 629, 1173, 1183, 1259, 1540, 1635, 1658, 1915, 1923
BshNI GGYRCC 2 cut(s) 135, 1444
BshTI ACCGGT 2 cut(s) 1113, 1134
BsiEI CGRYCG 6 cut(s) 720, 728, 756, 782, 1114, 1139
BsiHKAI GWGCWC 5 cut(s) 24, 298, 664, 1134, 1229
BsiHKCI CYCGRG 3 cut(s) 1889, 1948, 1962
BsiWI CGTACG 1 cut(s) 749
BslFI GGGAC 9 cut(s) 132, 593, 653, 985, 1131, 1301, 1663, 1715, 1923
BsmAI GTCTC 6 cut(s) 397, 550, 802, 868, 1390, 1584
BsmBI CGTCTC 3 cut(s) 397, 550, 802
BsmFI GGGAC 9 cut(s) 132, 593, 653, 985, 1131, 1301, 1663, 1715, 1923
BsmI GAATGC 1 cut(s) 830
BsnI GGCC 9 cut(s) 629, 1173, 1183, 1259, 1540, 1635, 1658, 1915, 1923
Bso31I GGTCTC 1 cut(s) 1584
BsoBI CYCGRG 3 cut(s) 1889, 1948, 1962
Bsp119I TTCGAA 1 cut(s) 219
Bsp1407I TGTACA 1 cut(s) 1742
Bsp19I CCATGG 3 cut(s) 131, 186, 311
Bsp68I TCGCGA 1 cut(s) 1832
BspANI GGCC 9 cut(s) 629, 1173, 1183, 1259, 1540, 1635, 1658, 1915, 1923
BspCNI CTCAG 1 cut(s) 1608
BspHI TCATGA 1 cut(s) 1027
BspMI ACCTGC 2 cut(s) 587, 1880
BspPI GGATC 6 cut(s) 250, 833, 1050, 1274, 1420, 1801
BspT104I TTCGAA 1 cut(s) 219
BspT107I GGYRCC 2 cut(s) 135, 1444
BspTNI GGTCTC 1 cut(s) 1584
BsrBI CCGCTC 1 cut(s) 1959
BsrDI GCAATG 2 cut(s) 308, 1662
BsrFI RCCGGY 8 cut(s) 625, 634, 1113, 1134, 1155, 1441, 1915, 1981
BsrGI TGTACA 1 cut(s) 1742
BsrI ACTGG 1 cut(s) 846
BssAI RCCGGY 8 cut(s) 625, 634, 1113, 1134, 1155, 1441, 1915, 1981
BssHII GCGCGC 3 cut(s) 2019, 2046, 2048
BssNI GRCGYC 5 cut(s) 807, 813, 1827, 1936, 2059
BssSI CACGAG 2 cut(s) 547, 663
BssT1I CCWWGG 7 cut(s) 131, 186, 277, 311, 1098, 1320, 1998
Bst2BI CACGAG 2 cut(s) 547, 663
Bst2UI CCWGG 4 cut(s) 359, 833, 1170, 1409
Bst4CI ACNGT 8 cut(s) 147, 506, 757, 783, 923, 1292, 1378, 2043
Bst6I CTCTTC 1 cut(s) 813
BstACI GRCGYC 5 cut(s) 807, 813, 1827, 1936, 2059
BstAPI GCANNNNNTGC 1 cut(s) 688
BstAUI TGTACA 1 cut(s) 1742
BstBAI YACGTR 2 cut(s) 84, 945
BstBI TTCGAA 1 cut(s) 219
BstDEI CTNAG 3 cut(s) 1546, 1595, 1643
BstDSI CCRYGG 4 cut(s) 131, 186, 311, 1630
BstEII GGTNACC 1 cut(s) 153
BstF5I GGATG 7 cut(s) 66, 259, 381, 563, 1330, 1426, 1623
BstH2I RGCGCY 1 cut(s) 1203
BstMAI GTCTC 6 cut(s) 397, 550, 802, 868, 1390, 1584
BstMCI CGRYCG 6 cut(s) 720, 728, 756, 782, 1114, 1139
BstNI CCWGG 4 cut(s) 359, 833, 1170, 1409
BstNSI RCATGY 1 cut(s) 286
BstPI GGTNACC 1 cut(s) 153
BstSLI GKGCMC 2 cut(s) 298, 1134
BstV1I GCAGC 5 cut(s) 50, 569, 754, 1804, 1889
BstV2I GAAGAC 4 cut(s) 696, 1369, 1670, 2060
BstXI CCANNNNNNTGG 2 cut(s) 187, 1060
BsuRI GGCC 9 cut(s) 629, 1173, 1183, 1259, 1540, 1635, 1658, 1915, 1923
BtgI CCRYGG 4 cut(s) 131, 186, 311, 1630
BtgZI GCGATG 1 cut(s) 34
BtrI CACGTC 2 cut(s) 407, 1907
BtsCI GGATG 7 cut(s) 66, 259, 381, 563, 1330, 1426, 1623
BtsIMutI CAGTG 1 cut(s) 1841
BtuMI TCGCGA 1 cut(s) 1832
BveI ACCTGC 2 cut(s) 587, 1880
CciI TCATGA 1 cut(s) 1027
Cfr10I RCCGGY 8 cut(s) 625, 634, 1113, 1134, 1155, 1441, 1915, 1981
Cfr13I GGNCC 6 cut(s) 1095, 1116, 1244, 1776, 1913, 1921
Cfr42I CCGCGG 1 cut(s) 1633
Cfr9I CCCGGG 2 cut(s) 1948, 1962
CpoI CGGWCCG 1 cut(s) 1116
CsiI ACCWGGT 1 cut(s) 831
Csp6I GTAC 4 cut(s) 515, 750, 1743, 1969
CspAI ACCGGT 2 cut(s) 1113, 1134
CspI CGGWCCG 1 cut(s) 1116
CviAII CATG 8 cut(s) 26, 132, 187, 283, 312, 879, 1028, 1536
CviQI GTAC 4 cut(s) 515, 750, 1743, 1969
DdeI CTNAG 3 cut(s) 1546, 1595, 1643
DrdI GACNNNNNNGTC 1 cut(s) 812
DseDI GACNNNNNNGTC 1 cut(s) 812
EaeI YGGCCR 4 cut(s) 1181, 1538, 1633, 1656
Eam1104I CTCTTC 1 cut(s) 813
EarI CTCTTC 1 cut(s) 813
EciI GGCGGA 3 cut(s) 1108, 1428, 1433
Eco130I CCWWGG 7 cut(s) 131, 186, 277, 311, 1098, 1320, 1998
Eco24I GRGCYC 5 cut(s) 337, 1006, 1360, 1933, 1957
Eco31I GGTCTC 1 cut(s) 1584
Eco47I GGWCC 4 cut(s) 1095, 1116, 1244, 1776
Eco72I CACGTG 1 cut(s) 84
Eco88I CYCGRG 3 cut(s) 1889, 1948, 1962
Eco91I GGTNACC 1 cut(s) 153
EcoO65I GGTNACC 1 cut(s) 153
EcoRI GAATTC 1 cut(s) 1689
EcoRII CCWGG 4 cut(s) 357, 831, 1168, 1407
EcoT14I CCWWGG 7 cut(s) 131, 186, 277, 311, 1098, 1320, 1998
EcoT22I ATGCAT 2 cut(s) 31, 884
EcoT38I GRGCYC 5 cut(s) 337, 1006, 1360, 1933, 1957
ErhI CCWWGG 7 cut(s) 131, 186, 277, 311, 1098, 1320, 1998
Esp3I CGTCTC 3 cut(s) 397, 550, 802
FaeI CATG 8 cut(s) 29, 135, 190, 286, 315, 882, 1031, 1539
FaqI GGGAC 9 cut(s) 132, 593, 653, 985, 1131, 1301, 1663, 1715, 1923
FatI CATG 8 cut(s) 25, 131, 186, 282, 311, 878, 1027, 1535
FauNDI CATATG 1 cut(s) 855
FbaI TGATCA 1 cut(s) 1858
FblI GTMKAC 4 cut(s) 778, 995, 1110, 1294
FokI GGATG 7 cut(s) 73, 266, 368, 570, 1337, 1433, 1610
FriOI GRGCYC 5 cut(s) 337, 1006, 1360, 1933, 1957
FspBI CTAG 6 cut(s) 56, 278, 933, 1382, 1608, 1695
FspI TGCGCA 2 cut(s) 553, 1876
GsaI CCCAGC 1 cut(s) 431
HaeII RGCGCY 1 cut(s) 1203
HaeIII GGCC 9 cut(s) 629, 1173, 1183, 1259, 1540, 1635, 1658, 1915, 1923
Hin1I GRCGYC 5 cut(s) 807, 813, 1827, 1936, 2059
Hin1II CATG 8 cut(s) 29, 135, 190, 286, 315, 882, 1031, 1539
HincII GTYRAC 6 cut(s) 779, 996, 1111, 1295, 1582, 1723
HindII GTYRAC 6 cut(s) 779, 996, 1111, 1295, 1582, 1723
HinfI GANTC 5 cut(s) 540, 1024, 1435, 1517, 1706
HpaI GTTAAC 1 cut(s) 1582
HphI GGTGA 2 cut(s) 147, 1793
Hpy188I TCNGA 8 cut(s) 329, 717, 725, 916, 1020, 1271, 1372, 1793
HpyAV CCTTC 6 cut(s) 324, 581, 962, 1159, 1270, 1934
HpyCH4III ACNGT 8 cut(s) 147, 506, 757, 783, 923, 1292, 1378, 2043
HpyCH4IV ACGT 9 cut(s) 83, 317, 406, 593, 813, 944, 1728, 1827, 1906
HpyF3I CTNAG 3 cut(s) 1546, 1595, 1643
HpySE526I ACGT 9 cut(s) 83, 317, 406, 593, 813, 944, 1728, 1827, 1906
Hsp92I GRCGYC 5 cut(s) 807, 813, 1827, 1936, 2059
Hsp92II CATG 8 cut(s) 29, 135, 190, 286, 315, 882, 1031, 1539
KroI GCCGGC 5 cut(s) 625, 634, 1155, 1441, 1915
KroNI GCCGGC 5 cut(s) 627, 636, 1157, 1443, 1917
Ksp22I TGATCA 1 cut(s) 1858
KspAI GTTAAC 1 cut(s) 1582
KspI CCGCGG 1 cut(s) 1633
Lsp1109I GCAGC 5 cut(s) 50, 569, 754, 1804, 1889
LweI GCATC 4 cut(s) 390, 506, 1078, 1390
MabI ACCWGGT 1 cut(s) 831
MaeI CTAG 6 cut(s) 56, 278, 933, 1382, 1608, 1695
MaeII ACGT 9 cut(s) 83, 317, 406, 593, 813, 944, 1728, 1827, 1906
MaeIII GTNAC 3 cut(s) 153, 1204, 1423
MauBI CGCGCGCG 1 cut(s) 2048
MbiI CCGCTC 1 cut(s) 1959
MboII GAAGA 6 cut(s) 696, 830, 1225, 1374, 1670, 2060
MfeI CAATTG 1 cut(s) 1668
MlsI TGGCCA 1 cut(s) 1658
MluCI AATT 8 cut(s) 232, 460, 928, 1177, 1217, 1573, 1668, 1689
MluI ACGCGT 2 cut(s) 531, 746
MluNI TGGCCA 1 cut(s) 1658
MlyI GAGTC 2 cut(s) 1429, 1715
MmeI TCCRAC 1 cut(s) 1073
Mox20I TGGCCA 1 cut(s) 1658
Mph1103I ATGCAT 2 cut(s) 31, 884
MreI CGCCGGCG 1 cut(s) 634
MroNI GCCGGC 5 cut(s) 625, 634, 1155, 1441, 1915
MroXI GAANNNNTTC 1 cut(s) 1898
MscI TGGCCA 1 cut(s) 1658
MseI TTAA 6 cut(s) 873, 1176, 1281, 1335, 1572, 1581
MslI CAYNNNNRTG 2 cut(s) 185, 301
Msp20I TGGCCA 1 cut(s) 1658
MspA1I CMGCKG 2 cut(s) 767, 1632
MunI CAATTG 1 cut(s) 1668
Mva1269I GAATGC 1 cut(s) 830
MvaI CCWGG 4 cut(s) 359, 833, 1170, 1409
NaeI GCCGGC 5 cut(s) 627, 636, 1157, 1443, 1917
NciI CCSGG 8 cut(s) 671, 1185, 1477, 1949, 1950, 1963, 1964, 2035
NcoI CCATGG 3 cut(s) 131, 186, 311
NdeI CATATG 1 cut(s) 855
NgoMIV GCCGGC 5 cut(s) 625, 634, 1155, 1441, 1915
NlaIII CATG 8 cut(s) 29, 135, 190, 286, 315, 882, 1031, 1539
NmeAIII GCCGAG 2 cut(s) 327, 1912
NmuCI GTSAC 1 cut(s) 153
NruI TCGCGA 1 cut(s) 1832
NsbI TGCGCA 2 cut(s) 553, 1876
NsiI ATGCAT 2 cut(s) 31, 884
NspI RCATGY 1 cut(s) 286
NspV TTCGAA 1 cut(s) 219
PagI TCATGA 1 cut(s) 1027
PauI GCGCGC 3 cut(s) 2019, 2046, 2048
PcsI WCGNNNNNNNCGW 7 cut(s) 412, 745, 749, 813, 1763, 1821, 1933
PctI GAATGC 1 cut(s) 830
PdiI GCCGGC 5 cut(s) 627, 636, 1157, 1443, 1917
PdmI GAANNNNTTC 1 cut(s) 1898
PfeI GAWTC 3 cut(s) 540, 1024, 1517
Pfl23II CGTACG 1 cut(s) 749
PflFI GACNNNGTC 1 cut(s) 1727
PfoI TCCNGGA 1 cut(s) 669
PinAI ACCGGT 2 cut(s) 1113, 1134
Ple19I CGATCG 2 cut(s) 720, 728
PleI GAGTC 2 cut(s) 1429, 1714
PmaCI CACGTG 1 cut(s) 84
PmlI CACGTG 1 cut(s) 84
PpsI GAGTC 2 cut(s) 1429, 1714
Ppu21I YACGTR 2 cut(s) 84, 945
PshBI ATTAAT 1 cut(s) 1335
Psp1406I AACGTT 1 cut(s) 593
Psp6I CCWGG 4 cut(s) 357, 831, 1168, 1407
PspCI CACGTG 1 cut(s) 84
PspEI GGTNACC 1 cut(s) 153
PspFI CCCAGC 1 cut(s) 427
PspGI CCWGG 4 cut(s) 357, 831, 1168, 1407
PspLI CGTACG 1 cut(s) 749
PspPI GGNCC 6 cut(s) 1095, 1116, 1244, 1776, 1913, 1921
PsyI GACNNNGTC 1 cut(s) 1727
PteI GCGCGC 3 cut(s) 2019, 2046, 2048
PvuI CGATCG 2 cut(s) 720, 728
RruI TCGCGA 1 cut(s) 1832
RsaI GTAC 4 cut(s) 516, 751, 1744, 1970
RsaNI GTAC 4 cut(s) 515, 750, 1743, 1969
RseI CAYNNNNRTG 2 cut(s) 185, 301
Rsr2I CGGWCCG 1 cut(s) 1116
RsrII CGGWCCG 1 cut(s) 1116
SacII CCGCGG 1 cut(s) 1633
SalI GTCGAC 4 cut(s) 777, 994, 1109, 1293
SaqAI TTAA 6 cut(s) 873, 1176, 1281, 1335, 1572, 1581
Sau96I GGNCC 6 cut(s) 1095, 1116, 1244, 1776, 1913, 1921
SchI GAGTC 2 cut(s) 1429, 1715
SexAI ACCWGGT 1 cut(s) 831
SfaNI GCATC 4 cut(s) 390, 506, 1078, 1390
Sfr303I CCGCGG 1 cut(s) 1633
SfuI TTCGAA 1 cut(s) 219
SgrAI CRCCGGYG 2 cut(s) 634, 1981
SgrBI CCGCGG 1 cut(s) 1633
SinI GGWCC 4 cut(s) 1095, 1116, 1244, 1776
SmaI CCCGGG 2 cut(s) 1950, 1964
SmiMI CAYNNNNRTG 2 cut(s) 185, 301
SmlI CTYRAG 1 cut(s) 373
SmoI CTYRAG 1 cut(s) 373
Sse9I AATT 8 cut(s) 232, 460, 928, 1177, 1217, 1573, 1668, 1689
SspMI CTAG 6 cut(s) 56, 278, 933, 1382, 1608, 1695
StyI CCWWGG 7 cut(s) 131, 186, 277, 311, 1098, 1320, 1998
TaaI ACNGT 8 cut(s) 147, 506, 757, 783, 923, 1292, 1378, 2043
TaiI ACGT 9 cut(s) 86, 320, 409, 596, 816, 947, 1731, 1830, 1909
TaqII GACCGA 1 cut(s) 1232
TasI AATT 8 cut(s) 232, 460, 928, 1177, 1217, 1573, 1668, 1689
TatI WGTACW 1 cut(s) 1742
TauI GCSGC 7 cut(s) 172, 346, 682, 1635, 1801, 2032, 2059
TfiI GAWTC 3 cut(s) 540, 1024, 1517
Tru1I TTAA 6 cut(s) 873, 1176, 1281, 1335, 1572, 1581
Tru9I TTAA 6 cut(s) 873, 1176, 1281, 1335, 1572, 1581
TscAI CASTG 1 cut(s) 1848
TseFI GTSAC 1 cut(s) 153
TseI GCWGC 5 cut(s) 38, 582, 767, 1817, 1877
Tsp45I GTSAC 1 cut(s) 153
TspDTI ATGAA 4 cut(s) 270, 514, 1016, 1321
TspGWI ACGGA 4 cut(s) 476, 784, 1055, 1567
TspMI CCCGGG 2 cut(s) 1948, 1962
TspRI CASTG 1 cut(s) 1848
Tth111I GACNNNGTC 1 cut(s) 1727
VneI GTGCAC 2 cut(s) 294, 1130
VpaK11BI GGWCC 4 cut(s) 1095, 1116, 1244, 1776
VspI ATTAAT 1 cut(s) 1335
XapI RAATTY 2 cut(s) 232, 1689
XbaI TCTAGA 1 cut(s) 932
XceI RCATGY 1 cut(s) 286
XmaI CCCGGG 2 cut(s) 1948, 1962
XmaJI CCTAGG 1 cut(s) 277
XmiI GTMKAC 4 cut(s) 778, 995, 1110, 1294
XmnI GAANNNNTTC 1 cut(s) 1898
XspI CTAG 6 cut(s) 56, 278, 933, 1382, 1608, 1695
ZraI GACGTC 2 cut(s) 814, 1828
Zsp2I ATGCAT 2 cut(s) 31, 884
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.