pycom12554g00070

protein Sb1514s002020 source

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012554
OG0002208
Physical Location & Seq
Reverse (-)
32722 .. 36235
3514 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12554g00070.5

Sequence Viewer

Length: 2113 bp
GCTCCGTGCTTCGGCATCGAACTGTCGGCTCGTACCCCGCTTCATCGACGCGTCGAGATTCGTCTCGTGCGCAGGATGGGTTTCTGTTTTGGCTACCTGCTGCGAAGGAACGTTGCCTCTCGTCGTCCCTTTCCTCCTCCTGCCGGCCTCGCCGGCGGGAGGACGACATCGTGGCGGTGCTCGTGTCCCGGACGAGCCGCACTCGTTGCGGTGCAGTTTCGGTCCTCGGGTTTCCGTTCGACATCTCGGATGCGGAACGCTGAAGGGGCGGTGGGTCTTCACGACCTCCGATCGTCCGATCGAAGCCTTGTCGCTCGACGCGTACGACCGTCGCGCCCGCTGCGAGCCGTCGACCGTCAAGGTCGTCCGTCTCGCGCGACCTCGTAGTTGGACCTTGGGTTGGGTCGACCGGTCCGCCTCTGGTGTGCACCGGTCGGCTCGTCCCTTCTGCCGGCGATACGCTCCTGGCCTTAATTGGCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGAATACATTAGCATGGGATAACATCATAGGATTTCGACGAACAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGGCGGATGTTACTTTTAGGACTCCGCCGGCACCTTATGAGAAATCAAAGTTTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGGTCATCCTCCGCGGCCAGCTTCTTAGAGGGACTATGGCCATCATTGCAATTGTTGGTCTTCAACGAGGAATTCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTCCTACCGATTGAATGGTCCGGTGAAGTGTTCGGATCGCGGCGACGCGGGTGGTTCGCTGCCCGCGACGTCCAGAACGACCCGAGAACCCGTTCCCACGTCGGGGGCCGGCGGGCCTTCGGGCTCGGCGTCCCCTTATCCCGGGAGCCCGCTCCCGGGCGTACCAACGAACACCGGCGCGTCTTTGCGCCAAGGAACCCGAACGAAAGAGCGCGCTCCCGCCGCCCCGGAAACGGTGCGCGCGCGGGCGGCGTCGTCGTCTTCTGTAAAGTCAAAACGACTCTCGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTTGTTGCGCCCGAAGCCGTTAGGCCGAGGGCACGCCTGCCTGGGCGTCACACGCCGTTGCCCCCCCGCGCCTCCCCCGGGAGCGTCGGGGGGCGGACGTTGGCCTCCCGTGCGTCCCCCCGCGCGGTTGGCACAAATGCCGGGTCCTCGGCGACGAACGCCACGACAATCGGTGGTTGTCGAACCTCGGTTGCCCGTTGTGCGCCTTCGTCGCGCCCGAGCGGCCCGCGACGCTCCCCGCTCCGCTTCGGCCGAGCTTTCAACGCGACCCCAGGTCAGGCGGGGTTACCCGCTGAATTTAAGCATATCAATAAGCGGAGGAAAAGAAACTTACGAGGATTCCCCTAGTAACGGCGAGCGAACCGGGAACAAGTGCTTGAAATTGTCGGGAGGGAAGCGGATGGGGGCTGGCGATGCGCCCCGGTCGGATGTGGAACGGTCGAAAGCCGGTCCGCCGATCGGCTCGGGGCGTGGACCGACGCGGATTGCGGCGGCGGCCAAAGCCCGGGCTGTTGATATGCCCGCGGAGACGCCGTCGACGCGATCGTGGAGGGCAGCGCGCGCCGTACCGGCGTGCTTCGGCAACTGCGCGCTCCCGGCGTCGGCCAGCGAGCTCCCCATTCGGCCCGTCTTGAAACACGGACCAAGGAGTCTGACATGCGTGCGAGTCAACGGGCGATCAAACCCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

704

Amino Acids

75.03

Weight (kDa)

11.74

Isoelectric Point (pI)

70.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2071
AatII GACGTC 1 cut(s) 1156
Acc16I TGCGCA 1 cut(s) 71
Acc36I ACCTGC 1 cut(s) 105
AccB1I GGYRCC 2 cut(s) 728, 809
AccBSI CCGCTC 3 cut(s) 1236, 1644, 1663
AccI GTMKAC 3 cut(s) 351, 406, 1959
AclI AACGTT 1 cut(s) 111
AclWI GGATC 2 cut(s) 704, 1127
AcoI YGGCCR 7 cut(s) 477, 864, 959, 982, 1672, 1918, 2026
AcsI RAATTY 2 cut(s) 1015, 1718
AcuI CTGAAG 1 cut(s) 282
AcyI GRCGYC 6 cut(s) 1153, 1213, 1336, 1468, 1953, 2022
AfaI GTAC 5 cut(s) 34, 324, 1070, 1247, 1990
AflIII ACRYGT 2 cut(s) 49, 319
AgeI ACCGGT 2 cut(s) 409, 430
AgsI TTSAA 6 cut(s) 509, 1008, 1098, 1684, 1802, 2057
AhdI GACNNNNNGTC 1 cut(s) 1695
AjiI CACGTC 1 cut(s) 1184
AjnI CCWGG 5 cut(s) 464, 691, 815, 1462, 1693
AjuI GAANNNNNNNTTGG 2 cut(s) 597, 629
AluBI AGCT 5 cut(s) 937, 965, 1041, 1679, 2036
AluI AGCT 5 cut(s) 937, 965, 1041, 1679, 2036
Alw21I GWGCWC 4 cut(s) 182, 430, 525, 2038
Alw26I GTCTC 5 cut(s) 68, 375, 674, 910, 1944
Alw44I GTGCAC 1 cut(s) 426
AlwI GGATC 2 cut(s) 704, 1127
Ama87I CYCGRG 8 cut(s) 226, 1166, 1225, 1239, 1499, 1639, 1886, 1927
ApaLI GTGCAC 1 cut(s) 426
ApeKI GCWGC 4 cut(s) 100, 340, 1143, 1977
ApoI RAATTY 2 cut(s) 1015, 1718
ArsI GACNNNNNNTTYG 2 cut(s) 1550, 1582
AseI ATTAAT 1 cut(s) 619
AsiGI ACCGGT 2 cut(s) 409, 430
Asp700I GAANNNNTTC 1 cut(s) 1175
AsuHPI GGTGA 1 cut(s) 1119
AvaI CYCGRG 8 cut(s) 226, 1166, 1225, 1239, 1499, 1639, 1886, 1927
AvaII GGWCC 8 cut(s) 222, 391, 412, 1102, 1566, 1872, 1896, 2064
BaeGI GKGCMC 3 cut(s) 430, 812, 1456
BalI TGGCCA 1 cut(s) 984
BanI GGYRCC 2 cut(s) 728, 809
BanII GRGCYC 4 cut(s) 644, 1210, 1234, 2038
BauI CACGAG 2 cut(s) 65, 181
BbsI GAAGAC 4 cut(s) 269, 653, 996, 1337
Bbv12I GWGCWC 4 cut(s) 182, 430, 525, 2038
BbvCI CCTCAGC 1 cut(s) 921
BbvI GCAGC 4 cut(s) 87, 327, 1130, 1989
BccI CCATC 3 cut(s) 70, 993, 1817
BceAI ACGGC 7 cut(s) 332, 851, 1424, 1462, 1790, 1940, 1971
BciT130I CCWGG 5 cut(s) 466, 693, 817, 1464, 1695
BcoDI GTCTC 5 cut(s) 68, 375, 674, 910, 1944
BfaI CTAG 4 cut(s) 666, 934, 1021, 1768
BfoI RGCGCY 1 cut(s) 499
BfuAI ACCTGC 1 cut(s) 105
BglI GCCNNNNNGGC 3 cut(s) 153, 1644, 1992
Bme18I GGWCC 8 cut(s) 222, 391, 412, 1102, 1566, 1872, 1896, 2064
BmeRI GACNNNNNGTC 1 cut(s) 1695
BmeT110I CYCGRG 8 cut(s) 226, 1166, 1225, 1239, 1499, 1639, 1886, 1927
BmgBI CACGTC 1 cut(s) 1184
BmiI GGNNCC 8 cut(s) 730, 758, 811, 826, 1191, 1231, 1281, 1567
BmsI GCATC 5 cut(s) 24, 240, 674, 1402, 1826
BpiI GAAGAC 4 cut(s) 269, 653, 996, 1337
BplI GAGNNNNNCTC 2 cut(s) 186, 218
Bpu10I CCTNAGC 1 cut(s) 921
Bsa29I ATCGAT 1 cut(s) 1396
BsaHI GRCGYC 6 cut(s) 1153, 1213, 1336, 1468, 1953, 2022
BsaI GGTCTC 1 cut(s) 910
BsaWI WCCGGW 3 cut(s) 409, 430, 1104
BsaXI ACNNNNNCTCC 2 cut(s) 1941, 1971
Bse3DI GCAATG 1 cut(s) 988
BseBI CCWGG 5 cut(s) 466, 693, 817, 1464, 1695
BseCI ATCGAT 1 cut(s) 1396
BseGI GGATG 7 cut(s) 81, 255, 614, 710, 949, 1828, 1856
BseMI GCAATG 1 cut(s) 988
BseMII CTCAG 1 cut(s) 935
BsePI GCGCGC 6 cut(s) 1296, 1323, 1325, 1980, 1982, 2011
BseRI GAGGAG 1 cut(s) 126
BseSI GKGCMC 3 cut(s) 430, 812, 1456
BseX3I CGGCCG 1 cut(s) 1672
BseXI GCAGC 4 cut(s) 87, 327, 1130, 1989
BsgI GTGCAG 1 cut(s) 233
BshNI GGYRCC 2 cut(s) 728, 809
BshTI ACCGGT 2 cut(s) 409, 430
BshVI ATCGAT 1 cut(s) 1396
BsiHKAI GWGCWC 4 cut(s) 182, 430, 525, 2038
BsiHKCI CYCGRG 8 cut(s) 226, 1166, 1225, 1239, 1499, 1639, 1886, 1927
BsiWI CGTACG 1 cut(s) 322
BslFI GGGAC 7 cut(s) 111, 171, 427, 989, 1041, 1200, 1522
BsmAI GTCTC 5 cut(s) 68, 375, 674, 910, 1944
BsmBI CGTCTC 3 cut(s) 68, 375, 1944
BsmFI GGGAC 7 cut(s) 111, 171, 427, 989, 1041, 1200, 1522
Bso31I GGTCTC 1 cut(s) 910
BsoBI CYCGRG 8 cut(s) 226, 1166, 1225, 1239, 1499, 1639, 1886, 1927
Bsp1286I GDGCHC 9 cut(s) 182, 430, 525, 644, 812, 1210, 1234, 1456, 2038
Bsp1407I TGTACA 1 cut(s) 1068
Bsp143I GATC 8 cut(s) 290, 298, 651, 696, 1119, 1879, 1965, 2100
BspCNI CTCAG 1 cut(s) 934
BspDI ATCGAT 1 cut(s) 1396
BspLI GGNNCC 8 cut(s) 730, 758, 811, 826, 1191, 1231, 1281, 1567
BspMI ACCTGC 1 cut(s) 105
BspPI GGATC 2 cut(s) 704, 1127
BspT107I GGYRCC 2 cut(s) 728, 809
BspTNI GGTCTC 1 cut(s) 910
BsrBI CCGCTC 3 cut(s) 1236, 1644, 1663
BsrDI GCAATG 1 cut(s) 988
BsrGI TGTACA 1 cut(s) 1068
BssHII GCGCGC 6 cut(s) 1296, 1323, 1325, 1980, 1982, 2011
BssMI GATC 8 cut(s) 290, 298, 651, 696, 1119, 1879, 1965, 2100
BssNI GRCGYC 6 cut(s) 1153, 1213, 1336, 1468, 1953, 2022
BssSI CACGAG 2 cut(s) 65, 181
BssT1I CCWWGG 4 cut(s) 394, 604, 1275, 2067
Bst2BI CACGAG 2 cut(s) 65, 181
Bst2UI CCWGG 5 cut(s) 466, 693, 817, 1464, 1695
Bst4CI ACNGT 6 cut(s) 24, 330, 356, 662, 1320, 1861
BstACI GRCGYC 6 cut(s) 1153, 1213, 1336, 1468, 1953, 2022
BstAPI GCANNNNNTGC 1 cut(s) 206
BstAUI TGTACA 1 cut(s) 1068
BstDEI CTNAG 3 cut(s) 872, 921, 969
BstDSI CCRYGG 2 cut(s) 956, 1945
BstEII GGTNACC 1 cut(s) 1707
BstF5I GGATG 7 cut(s) 81, 255, 614, 710, 949, 1828, 1856
BstH2I RGCGCY 1 cut(s) 499
BstKTI GATC 8 cut(s) 293, 301, 654, 699, 1122, 1882, 1968, 2103
BstMAI GTCTC 5 cut(s) 68, 375, 674, 910, 1944
BstMBI GATC 8 cut(s) 290, 298, 651, 696, 1119, 1879, 1965, 2100
BstNI CCWGG 5 cut(s) 466, 693, 817, 1464, 1695
BstNSI RCATGY 1 cut(s) 2083
BstPI GGTNACC 1 cut(s) 1707
BstSLI GKGCMC 3 cut(s) 430, 812, 1456
BstV1I GCAGC 4 cut(s) 87, 327, 1130, 1989
BstV2I GAAGAC 4 cut(s) 269, 653, 996, 1337
BstZI CGGCCG 1 cut(s) 1672
Bsu15I ATCGAT 1 cut(s) 1396
BsuTUI ATCGAT 1 cut(s) 1396
BtgI CCRYGG 2 cut(s) 956, 1945
BtgZI GCGATG 1 cut(s) 1849
BtrI CACGTC 1 cut(s) 1184
BtsCI GGATG 7 cut(s) 81, 255, 614, 710, 949, 1828, 1856
BveI ACCTGC 1 cut(s) 105
Cfr42I CCGCGG 2 cut(s) 959, 1948
Cfr9I CCCGGG 4 cut(s) 1225, 1239, 1499, 1927
ClaI ATCGAT 1 cut(s) 1396
CpoI CGGWCCG 2 cut(s) 412, 1872
Csp6I GTAC 5 cut(s) 33, 323, 1069, 1246, 1989
CspAI ACCGGT 2 cut(s) 409, 430
CspI CGGWCCG 2 cut(s) 412, 1872
CviAII CATG 3 cut(s) 556, 862, 2080
CviQI GTAC 5 cut(s) 33, 323, 1069, 1246, 1989
DdeI CTNAG 3 cut(s) 872, 921, 969
DpnI GATC 8 cut(s) 292, 300, 653, 698, 1121, 1881, 1967, 2102
DpnII GATC 8 cut(s) 290, 298, 651, 696, 1119, 1879, 1965, 2100
DrdI GACNNNNNNGTC 1 cut(s) 2071
DriI GACNNNNNGTC 1 cut(s) 1695
DseDI GACNNNNNNGTC 1 cut(s) 2071
EaeI YGGCCR 7 cut(s) 477, 864, 959, 982, 1672, 1918, 2026
EagI CGGCCG 1 cut(s) 1672
Eam1105I GACNNNNNGTC 1 cut(s) 1695
EciI GGCGGA 5 cut(s) 404, 712, 717, 1531, 1864
Ecl136II GAGCTC 1 cut(s) 2036
EclXI CGGCCG 1 cut(s) 1672
Eco130I CCWWGG 4 cut(s) 394, 604, 1275, 2067
Eco24I GRGCYC 4 cut(s) 644, 1210, 1234, 2038
Eco31I GGTCTC 1 cut(s) 910
Eco32I GATATC 1 cut(s) 1380
Eco47I GGWCC 8 cut(s) 222, 391, 412, 1102, 1566, 1872, 1896, 2064
Eco52I CGGCCG 1 cut(s) 1672
Eco53kI GAGCTC 1 cut(s) 2036
Eco57I CTGAAG 1 cut(s) 282
Eco88I CYCGRG 8 cut(s) 226, 1166, 1225, 1239, 1499, 1639, 1886, 1927
Eco91I GGTNACC 1 cut(s) 1707
EcoICRI GAGCTC 1 cut(s) 2036
EcoO109I RGGNCCY 1 cut(s) 1566
EcoO65I GGTNACC 1 cut(s) 1707
EcoRI GAATTC 1 cut(s) 1015
EcoRII CCWGG 5 cut(s) 464, 691, 815, 1462, 1693
EcoRV GATATC 1 cut(s) 1380
EcoT14I CCWWGG 4 cut(s) 394, 604, 1275, 2067
EcoT38I GRGCYC 4 cut(s) 644, 1210, 1234, 2038
ErhI CCWWGG 4 cut(s) 394, 604, 1275, 2067
Esp3I CGTCTC 3 cut(s) 68, 375, 1944
FaeI CATG 3 cut(s) 559, 865, 2083
FaqI GGGAC 7 cut(s) 111, 171, 427, 989, 1041, 1200, 1522
FatI CATG 3 cut(s) 555, 861, 2079
FblI GTMKAC 3 cut(s) 351, 406, 1959
FokI GGATG 7 cut(s) 88, 262, 621, 717, 936, 1835, 1863
FriOI GRGCYC 4 cut(s) 644, 1210, 1234, 2038
FspBI CTAG 4 cut(s) 666, 934, 1021, 1768
FspI TGCGCA 1 cut(s) 71
HaeII RGCGCY 1 cut(s) 499
Hin1I GRCGYC 6 cut(s) 1153, 1213, 1336, 1468, 1953, 2022
Hin1II CATG 3 cut(s) 559, 865, 2083
HincII GTYRAC 6 cut(s) 352, 407, 908, 1049, 1960, 2093
HindII GTYRAC 6 cut(s) 352, 407, 908, 1049, 1960, 2093
HinfI GANTC 8 cut(s) 58, 719, 843, 1032, 1364, 1761, 2072, 2089
HpaI GTTAAC 1 cut(s) 908
HphI GGTGA 1 cut(s) 1119
Hpy166II GTNNAC 9 cut(s) 352, 407, 428, 908, 1049, 1071, 1896, 1960, 2093
Hpy188I TCNGA 8 cut(s) 249, 290, 298, 544, 656, 1119, 1850, 2077
Hpy188III TCNNGA 7 cut(s) 55, 281, 624, 840, 1157, 1810, 2054
Hpy8I GTNNAC 9 cut(s) 352, 407, 428, 908, 1049, 1071, 1896, 1960, 2093
HpyAV CCTTC 6 cut(s) 99, 257, 455, 799, 1211, 1638
HpyCH4III ACNGT 6 cut(s) 24, 330, 356, 662, 1320, 1861
HpyCH4IV ACGT 6 cut(s) 111, 1054, 1153, 1183, 1406, 1520
HpyCH4V TGCA 4 cut(s) 214, 428, 861, 993
HpyF3I CTNAG 3 cut(s) 872, 921, 969
HpySE526I ACGT 6 cut(s) 111, 1054, 1153, 1183, 1406, 1520
Hsp92I GRCGYC 6 cut(s) 1153, 1213, 1336, 1468, 1953, 2022
Hsp92II CATG 3 cut(s) 559, 865, 2083
KroI GCCGGC 5 cut(s) 143, 152, 451, 725, 1192
KroNI GCCGGC 5 cut(s) 145, 154, 453, 727, 1194
KspAI GTTAAC 1 cut(s) 908
KspI CCGCGG 2 cut(s) 959, 1948
Kzo9I GATC 8 cut(s) 290, 298, 651, 696, 1119, 1879, 1965, 2100
Lsp1109I GCAGC 4 cut(s) 87, 327, 1130, 1989
LweI GCATC 5 cut(s) 24, 240, 674, 1402, 1826
MaeI CTAG 4 cut(s) 666, 934, 1021, 1768
MaeII ACGT 6 cut(s) 111, 1054, 1153, 1183, 1406, 1520
MaeIII GTNAC 5 cut(s) 500, 707, 1469, 1707, 1770
MalI GATC 8 cut(s) 292, 300, 653, 698, 1121, 1881, 1967, 2102
MauBI CGCGCGCG 1 cut(s) 1325
MbiI CCGCTC 3 cut(s) 1236, 1644, 1663
MboI GATC 8 cut(s) 290, 298, 651, 696, 1119, 1879, 1965, 2100
MboII GAAGA 6 cut(s) 269, 521, 658, 996, 1337, 1413
MfeI CAATTG 1 cut(s) 994
MhlI GDGCHC 9 cut(s) 182, 430, 525, 644, 812, 1210, 1234, 1456, 2038
MlsI TGGCCA 1 cut(s) 984
MluCI AATT 8 cut(s) 473, 513, 796, 899, 994, 1015, 1718, 1803
MluI ACGCGT 2 cut(s) 49, 319
MluNI TGGCCA 1 cut(s) 984
MlyI GAGTC 5 cut(s) 713, 1041, 1358, 2081, 2098
MmeI TCCRAC 2 cut(s) 369, 1828
Mox20I TGGCCA 1 cut(s) 984
MreI CGCCGGCG 1 cut(s) 152
MroNI GCCGGC 5 cut(s) 143, 152, 451, 725, 1192
MroXI GAANNNNTTC 1 cut(s) 1175
MscI TGGCCA 1 cut(s) 984
MseI TTAA 6 cut(s) 472, 619, 790, 898, 907, 1722
MslI CAYNNNNRTG 1 cut(s) 554
Msp20I TGGCCA 1 cut(s) 984
MspA1I CMGCKG 4 cut(s) 340, 958, 1715, 1947
MunI CAATTG 1 cut(s) 994
MvaI CCWGG 5 cut(s) 466, 693, 817, 1464, 1695
NaeI GCCGGC 5 cut(s) 145, 154, 453, 727, 1194
NdeII GATC 8 cut(s) 290, 298, 651, 696, 1119, 1879, 1965, 2100
NgoMIV GCCGGC 5 cut(s) 143, 152, 451, 725, 1192
NlaIII CATG 3 cut(s) 559, 865, 2083
NlaIV GGNNCC 8 cut(s) 730, 758, 811, 826, 1191, 1231, 1281, 1567
NmeAIII GCCGAG 6 cut(s) 1189, 1348, 1363, 1473, 1550, 1700
NmuCI GTSAC 1 cut(s) 1469
NsbI TGCGCA 1 cut(s) 71
NspI RCATGY 1 cut(s) 2083
PauI GCGCGC 6 cut(s) 1296, 1323, 1325, 1980, 1982, 2011
PcsI WCGNNNNNNNCGW 9 cut(s) 318, 322, 1089, 1147, 1159, 1210, 1583, 1637, 1965
PdiI GCCGGC 5 cut(s) 145, 154, 453, 727, 1194
PdmI GAANNNNTTC 1 cut(s) 1175
PfeI GAWTC 3 cut(s) 58, 843, 1761
Pfl23II CGTACG 1 cut(s) 322
PflFI GACNNNGTC 2 cut(s) 1053, 1955
PfoI TCCNGGA 1 cut(s) 187
PinAI ACCGGT 2 cut(s) 409, 430
Ple19I CGATCG 4 cut(s) 293, 301, 1882, 1968
PleI GAGTC 5 cut(s) 713, 1040, 1358, 2080, 2097
PpsI GAGTC 5 cut(s) 713, 1040, 1358, 2080, 2097
PpuMI RGGWCCY 1 cut(s) 1566
PshBI ATTAAT 1 cut(s) 619
Psp124BI GAGCTC 1 cut(s) 2038
Psp1406I AACGTT 1 cut(s) 111
Psp5II RGGWCCY 1 cut(s) 1566
Psp6I CCWGG 5 cut(s) 464, 691, 815, 1462, 1693
PspEI GGTNACC 1 cut(s) 1707
PspGI CCWGG 5 cut(s) 464, 691, 815, 1462, 1693
PspLI CGTACG 1 cut(s) 322
PspN4I GGNNCC 8 cut(s) 730, 758, 811, 826, 1191, 1231, 1281, 1567
PspPPI RGGWCCY 1 cut(s) 1566
PsyI GACNNNGTC 2 cut(s) 1053, 1955
PteI GCGCGC 6 cut(s) 1296, 1323, 1325, 1980, 1982, 2011
PvuI CGATCG 4 cut(s) 293, 301, 1882, 1968
RsaI GTAC 5 cut(s) 34, 324, 1070, 1247, 1990
RsaNI GTAC 5 cut(s) 33, 323, 1069, 1246, 1989
RseI CAYNNNNRTG 1 cut(s) 554
Rsr2I CGGWCCG 2 cut(s) 412, 1872
RsrII CGGWCCG 2 cut(s) 412, 1872
SacI GAGCTC 1 cut(s) 2038
SacII CCGCGG 2 cut(s) 959, 1948
SalI GTCGAC 3 cut(s) 350, 405, 1958
SaqAI TTAA 6 cut(s) 472, 619, 790, 898, 907, 1722
Sau3AI GATC 8 cut(s) 290, 298, 651, 696, 1119, 1879, 1965, 2100
SchI GAGTC 5 cut(s) 713, 1041, 1358, 2081, 2098
SduI GDGCHC 9 cut(s) 182, 430, 525, 644, 812, 1210, 1234, 1456, 2038
SfaNI GCATC 5 cut(s) 24, 240, 674, 1402, 1826
Sfr303I CCGCGG 2 cut(s) 959, 1948
SgrAI CRCCGGYG 2 cut(s) 152, 1258
SgrBI CCGCGG 2 cut(s) 959, 1948
SgrDI CGTCGACG 1 cut(s) 1958
SinI GGWCC 8 cut(s) 222, 391, 412, 1102, 1566, 1872, 1896, 2064
SmaI CCCGGG 4 cut(s) 1227, 1241, 1501, 1929
SmiMI CAYNNNNRTG 1 cut(s) 554
SrfI GCCCGGGC 1 cut(s) 1929
Sse9I AATT 8 cut(s) 473, 513, 796, 899, 994, 1015, 1718, 1803
SspMI CTAG 4 cut(s) 666, 934, 1021, 1768
SstI GAGCTC 1 cut(s) 2038
StyI CCWWGG 4 cut(s) 394, 604, 1275, 2067
TaaI ACNGT 6 cut(s) 24, 330, 356, 662, 1320, 1861
TaiI ACGT 6 cut(s) 114, 1057, 1156, 1186, 1409, 1523
TaqII GACCGA 2 cut(s) 210, 1913
TasI AATT 8 cut(s) 473, 513, 796, 899, 994, 1015, 1718, 1803
TatI WGTACW 1 cut(s) 1068
TauI GCSGC 9 cut(s) 200, 961, 1127, 1309, 1336, 1647, 1914, 1917, 1920
TfiI GAWTC 3 cut(s) 58, 843, 1761
Tru1I TTAA 6 cut(s) 472, 619, 790, 898, 907, 1722
Tru9I TTAA 6 cut(s) 472, 619, 790, 898, 907, 1722
TseFI GTSAC 1 cut(s) 1469
TseI GCWGC 4 cut(s) 100, 340, 1143, 1977
Tsp45I GTSAC 1 cut(s) 1469
TspDTI ATGAA 3 cut(s) 32, 605, 1414
TspGWI ACGGA 6 cut(s) 224, 357, 817, 893, 1390, 2077
TspMI CCCGGG 4 cut(s) 1225, 1239, 1499, 1927
Tth111I GACNNNGTC 2 cut(s) 1053, 1955
VneI GTGCAC 1 cut(s) 426
VpaK11BI GGWCC 8 cut(s) 222, 391, 412, 1102, 1566, 1872, 1896, 2064
VspI ATTAAT 1 cut(s) 619
XapI RAATTY 2 cut(s) 1015, 1718
XceI RCATGY 1 cut(s) 2083
XmaI CCCGGG 4 cut(s) 1225, 1239, 1499, 1927
XmiI GTMKAC 3 cut(s) 351, 406, 1959
XmnI GAANNNNTTC 1 cut(s) 1175
XspI CTAG 4 cut(s) 666, 934, 1021, 1768
ZraI GACGTC 1 cut(s) 1154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.