pycom12597g00050

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012597
OG0002208
Physical Location & Seq
Forward (+)
20574 .. 33694
13121 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12597g00050.11

Sequence Viewer

Length: 1809 bp
ATGCTATATAGGGGGGTGGTGCTCCATGCATTTCAAGCAGCCTGCACCATCGCTCTAGGGATGACATTCTCGACCATCATCACGTGTCGCCTCGGTATATATGATGTGTCGGGTTTGGGACATTCCCGCCCATGGTGCCGACGACGGTGGCGGTCACCCCCGCCACCGCCGCCGCCCCCACCACCATGGGTGCGATTTCGTTTTTTTTTTTTTTTTTCGAAAAAAAAAAAAAAAATTTGTTTTCATACGAAACAAAATTTTTTACTAACGCGACGGCGTATGAGTGGTAATTGGGTTGTATCCGACGGCAGGCTCCGTGCTTCGGCATCGAACTGTCGGCTCGTACCCCGCTTCATCGACGCATCGAGATTCGTCTCGTGCGCAAGATGGGTTTCTGTTTTGGCTACCTGCTGCGAAGGAACGTTGCCTCTCGTCGTCCCTTTCCTCCTCCTGCCGGCCTTGCCGGCGGGAGGACGACATAGTGGCGGTGCTCGTGTCCCGGACGAGCCGCACTCGTTGCGGCGCGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGGCGGATGTTACTTTTAGGACTCCGCCGGCACCTTATGAGAAATCAAAGTTTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGGTGGCTGCTGCGAAGGAACGTTGCCTCTCGTCGTCCCTTTCCTCCTCCTGCCGGCCTTGCCGGCGGGAGGACGACATAGTGGCGGTGCTCGTGTCCCGGACGAGCCGCACTCGTTGCGGTGCAGTTTCGGTCCTCGGGTTTCCGTTCGACATCTCGGATCGTCCGATCGAAGCCTTGTCGCTCGACGCGTACGACCGTCGCGCCCGCTGCGAGCCGTCGACCGTCAAGGTCGTCCGTCTCGCGCGACGCCGACGTCGAAGAGGAATGCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCGGATGTTACTTTTAGGACTCCGCCGGCACCTTATGAGAAATCAAAGTTTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGGTCATCCTCCGCGGCCAGCTTCTTAGAGGGACTATGGCCATCATTGCAATTGTTGGTCTTCAACGAGGAATTCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTCCTACCGATTGAATGGTCCGGTGAAGTGTTCGGATCGCGGCGACGCGGGTGGTTCGCTGCCCGCGACGTCGCGAGAAGTCCACTGAACCTTATCATTCAGAACGACCCGAGAACCCGTTCCGACGTCGGGGGCCGGCGGGCCTTCGGGCTCGGCGTCCCCTTATCCCGGGAGCCCGCTCCCGGGCGTACCAACGAACACCGGCGCGTCTTTGCGCCAAGGAACCCGAACGAAAGAGCGCGCTCCCGCCGCCCCGGAAACGGTGCCGCGCGGGCGGCGTCGTCGTCTTCTGTAAAAGTCAAAACGACTCTCGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

603

Amino Acids

66.0

Weight (kDa)

11.35

Isoelectric Point (pI)

60.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 976
AatII GACGTC 3 cut(s) 980, 1506, 1563
Acc16I TGCGCA 1 cut(s) 382
Acc36I ACCTGC 1 cut(s) 416
AccB1I GGYRCC 5 cut(s) 135, 643, 1078, 1159, 1697
AccBSI CCGCTC 1 cut(s) 1613
AccI GTMKAC 1 cut(s) 942
AclI AACGTT 2 cut(s) 422, 743
AclWI GGATC 4 cut(s) 619, 889, 997, 1477
AcoI YGGCCR 3 cut(s) 1214, 1309, 1332
AcsI RAATTY 3 cut(s) 234, 256, 1365
AcvI CACGTG 1 cut(s) 84
AcyI GRCGYC 6 cut(s) 971, 977, 1503, 1560, 1590, 1712
AfaI GTAC 4 cut(s) 345, 915, 1420, 1624
AflIII ACRYGT 2 cut(s) 83, 910
AgsI TTSAA 3 cut(s) 35, 1358, 1448
AjnI CCWGG 3 cut(s) 606, 995, 1165
AluBI AGCT 3 cut(s) 1287, 1315, 1391
AluI AGCT 3 cut(s) 1287, 1315, 1391
Alw21I GWGCWC 3 cut(s) 24, 493, 814
Alw26I GTCTC 5 cut(s) 379, 589, 966, 1032, 1260
AlwI GGATC 4 cut(s) 619, 889, 997, 1477
Ama87I CYCGRG 4 cut(s) 858, 1543, 1602, 1616
AoxI GGCC 7 cut(s) 456, 777, 1214, 1309, 1332, 1567, 1575
ApeKI GCWGC 6 cut(s) 38, 411, 729, 732, 931, 1493
ApoI RAATTY 3 cut(s) 234, 256, 1365
AseI ATTAAT 1 cut(s) 534
Asp700I GAANNNNTTC 1 cut(s) 1552
AspS9I GGNCC 4 cut(s) 854, 1452, 1567, 1575
AsuC2I CCSGG 9 cut(s) 500, 676, 821, 1111, 1603, 1604, 1617, 1618, 1689
AsuHPI GGTGA 2 cut(s) 147, 1469
AsuII TTCGAA 1 cut(s) 218
AvaI CYCGRG 4 cut(s) 858, 1543, 1602, 1616
AvaII GGWCC 2 cut(s) 854, 1452
BaeGI GKGCMC 1 cut(s) 1162
BalI TGGCCA 1 cut(s) 1334
BanI GGYRCC 5 cut(s) 135, 643, 1078, 1159, 1697
BanII GRGCYC 3 cut(s) 559, 1587, 1611
BauI CACGAG 3 cut(s) 376, 492, 813
BbrPI CACGTG 1 cut(s) 84
BbsI GAAGAC 3 cut(s) 568, 1346, 1713
Bbv12I GWGCWC 3 cut(s) 24, 493, 814
BbvCI CCTCAGC 1 cut(s) 1271
BbvI GCAGC 6 cut(s) 50, 398, 716, 719, 918, 1480
BccI CCATC 4 cut(s) 56, 83, 381, 1343
BceAI ACGGC 4 cut(s) 290, 322, 923, 1201
BciT130I CCWGG 3 cut(s) 608, 997, 1167
BciVI GTATCC 1 cut(s) 310
BcnI CCSGG 9 cut(s) 500, 676, 821, 1111, 1603, 1604, 1617, 1618, 1689
BcoDI GTCTC 5 cut(s) 379, 589, 966, 1032, 1260
BfaI CTAG 4 cut(s) 56, 581, 1284, 1371
BfuAI ACCTGC 1 cut(s) 416
BfuI GTATCC 1 cut(s) 310
BglI GCCNNNNNGGC 3 cut(s) 464, 785, 1706
Bme18I GGWCC 2 cut(s) 854, 1452
BmeT110I CYCGRG 4 cut(s) 858, 1543, 1602, 1616
BmgT120I GGNCC 4 cut(s) 854, 1452, 1567, 1575
BmsI GCATC 4 cut(s) 335, 371, 589, 1779
BpiI GAAGAC 3 cut(s) 568, 1346, 1713
BplI GAGNNNNNCTC 4 cut(s) 497, 529, 818, 850
Bpu10I CCTNAGC 1 cut(s) 1271
Bpu14I TTCGAA 1 cut(s) 218
BpuMI CCSGG 9 cut(s) 500, 676, 821, 1111, 1603, 1604, 1617, 1618, 1689
Bsa29I ATCGAT 1 cut(s) 1773
BsaAI YACGTR 1 cut(s) 84
BsaHI GRCGYC 6 cut(s) 971, 977, 1503, 1560, 1590, 1712
BsaI GGTCTC 1 cut(s) 1260
BsaWI WCCGGW 1 cut(s) 1454
Bse118I RCCGGY 8 cut(s) 454, 463, 640, 775, 784, 1075, 1569, 1635
Bse1I ACTGG 1 cut(s) 1010
Bse3DI GCAATG 1 cut(s) 1338
BseBI CCWGG 3 cut(s) 608, 997, 1167
BseCI ATCGAT 1 cut(s) 1773
BseGI GGATG 4 cut(s) 66, 625, 1060, 1299
BseMI GCAATG 1 cut(s) 1338
BseMII CTCAG 1 cut(s) 1285
BseNI ACTGG 1 cut(s) 1010
BsePI GCGCGC 1 cut(s) 1673
BseRI GAGGAG 2 cut(s) 437, 758
BseSI GKGCMC 1 cut(s) 1162
BseXI GCAGC 6 cut(s) 50, 398, 716, 719, 918, 1480
BsgI GTGCAG 2 cut(s) 28, 865
Bsh1285I CGRYCG 3 cut(s) 892, 920, 946
BshFI GGCC 7 cut(s) 458, 779, 1216, 1311, 1334, 1569, 1577
BshNI GGYRCC 5 cut(s) 135, 643, 1078, 1159, 1697
BshVI ATCGAT 1 cut(s) 1773
BsiEI CGRYCG 3 cut(s) 892, 920, 946
BsiHKAI GWGCWC 3 cut(s) 24, 493, 814
BsiHKCI CYCGRG 4 cut(s) 858, 1543, 1602, 1616
BsiWI CGTACG 1 cut(s) 913
BslFI GGGAC 8 cut(s) 132, 422, 482, 743, 803, 1339, 1391, 1577
BsmAI GTCTC 5 cut(s) 379, 589, 966, 1032, 1260
BsmBI CGTCTC 2 cut(s) 379, 966
BsmFI GGGAC 8 cut(s) 132, 422, 482, 743, 803, 1339, 1391, 1577
BsmI GAATGC 1 cut(s) 994
BsnI GGCC 7 cut(s) 458, 779, 1216, 1311, 1334, 1569, 1577
Bso31I GGTCTC 1 cut(s) 1260
BsoBI CYCGRG 4 cut(s) 858, 1543, 1602, 1616
Bsp119I TTCGAA 1 cut(s) 218
Bsp1286I GDGCHC 7 cut(s) 24, 493, 559, 814, 1162, 1587, 1611
Bsp1407I TGTACA 1 cut(s) 1418
Bsp143I GATC 6 cut(s) 566, 611, 881, 889, 1002, 1469
Bsp19I CCATGG 2 cut(s) 131, 185
Bsp68I TCGCGA 1 cut(s) 1508
BspANI GGCC 7 cut(s) 458, 779, 1216, 1311, 1334, 1569, 1577
BspCNI CTCAG 1 cut(s) 1284
BspDI ATCGAT 1 cut(s) 1773
BspMI ACCTGC 1 cut(s) 416
BspPI GGATC 4 cut(s) 619, 889, 997, 1477
BspT104I TTCGAA 1 cut(s) 218
BspT107I GGYRCC 5 cut(s) 135, 643, 1078, 1159, 1697
BspTNI GGTCTC 1 cut(s) 1260
BsrBI CCGCTC 1 cut(s) 1613
BsrDI GCAATG 1 cut(s) 1338
BsrFI RCCGGY 8 cut(s) 454, 463, 640, 775, 784, 1075, 1569, 1635
BsrGI TGTACA 1 cut(s) 1418
BsrI ACTGG 1 cut(s) 1010
BssAI RCCGGY 8 cut(s) 454, 463, 640, 775, 784, 1075, 1569, 1635
BssHII GCGCGC 1 cut(s) 1673
BssMI GATC 6 cut(s) 566, 611, 881, 889, 1002, 1469
BssNI GRCGYC 6 cut(s) 971, 977, 1503, 1560, 1590, 1712
BssSI CACGAG 3 cut(s) 376, 492, 813
BssT1I CCWWGG 3 cut(s) 131, 185, 1652
Bst2BI CACGAG 3 cut(s) 376, 492, 813
Bst2UI CCWGG 3 cut(s) 608, 997, 1167
Bst4CI ACNGT 6 cut(s) 147, 335, 577, 921, 947, 1697
Bst6I CTCTTC 1 cut(s) 977
BstACI GRCGYC 6 cut(s) 971, 977, 1503, 1560, 1590, 1712
BstAPI GCANNNNNTGC 2 cut(s) 517, 838
BstAUI TGTACA 1 cut(s) 1418
BstBAI YACGTR 1 cut(s) 84
BstBI TTCGAA 1 cut(s) 218
BstDEI CTNAG 3 cut(s) 1222, 1271, 1319
BstDSI CCRYGG 3 cut(s) 131, 185, 1306
BstEII GGTNACC 1 cut(s) 153
BstF5I GGATG 4 cut(s) 66, 625, 1060, 1299
BstKTI GATC 6 cut(s) 569, 614, 884, 892, 1005, 1472
BstMAI GTCTC 5 cut(s) 379, 589, 966, 1032, 1260
BstMBI GATC 6 cut(s) 566, 611, 881, 889, 1002, 1469
BstMCI CGRYCG 3 cut(s) 892, 920, 946
BstNI CCWGG 3 cut(s) 608, 997, 1167
BstNSI RCATGY 1 cut(s) 1050
BstPI GGTNACC 1 cut(s) 153
BstSLI GKGCMC 1 cut(s) 1162
BstV1I GCAGC 6 cut(s) 50, 398, 716, 719, 918, 1480
BstV2I GAAGAC 3 cut(s) 568, 1346, 1713
BstXI CCANNNNNNTGG 1 cut(s) 186
Bsu15I ATCGAT 1 cut(s) 1773
BsuI GTATCC 1 cut(s) 310
BsuRI GGCC 7 cut(s) 458, 779, 1216, 1311, 1334, 1569, 1577
BsuTUI ATCGAT 1 cut(s) 1773
BtgI CCRYGG 3 cut(s) 131, 185, 1306
BtgZI GCGATG 1 cut(s) 34
BtsCI GGATG 4 cut(s) 66, 625, 1060, 1299
BtsIMutI CAGTG 1 cut(s) 1517
BtuMI TCGCGA 1 cut(s) 1508
BveI ACCTGC 1 cut(s) 416
Cfr10I RCCGGY 8 cut(s) 454, 463, 640, 775, 784, 1075, 1569, 1635
Cfr13I GGNCC 4 cut(s) 854, 1452, 1567, 1575
Cfr42I CCGCGG 1 cut(s) 1309
Cfr9I CCCGGG 2 cut(s) 1602, 1616
ClaI ATCGAT 1 cut(s) 1773
CseI GACGC 7 cut(s) 368, 918, 979, 1488, 1579, 1630, 1701
CsiI ACCWGGT 1 cut(s) 995
Csp6I GTAC 4 cut(s) 344, 914, 1419, 1623
CviAII CATG 6 cut(s) 26, 132, 186, 1043, 1047, 1212
CviQI GTAC 4 cut(s) 344, 914, 1419, 1623
DdeI CTNAG 3 cut(s) 1222, 1271, 1319
DpnI GATC 6 cut(s) 568, 613, 883, 891, 1004, 1471
DpnII GATC 6 cut(s) 566, 611, 881, 889, 1002, 1469
DrdI GACNNNNNNGTC 1 cut(s) 976
DseDI GACNNNNNNGTC 1 cut(s) 976
EaeI YGGCCR 3 cut(s) 1214, 1309, 1332
Eam1104I CTCTTC 1 cut(s) 977
EarI CTCTTC 1 cut(s) 977
EciI GGCGGA 3 cut(s) 627, 632, 1062
Eco130I CCWWGG 3 cut(s) 131, 185, 1652
Eco24I GRGCYC 3 cut(s) 559, 1587, 1611
Eco31I GGTCTC 1 cut(s) 1260
Eco32I GATATC 1 cut(s) 1757
Eco47I GGWCC 2 cut(s) 854, 1452
Eco72I CACGTG 1 cut(s) 84
Eco88I CYCGRG 4 cut(s) 858, 1543, 1602, 1616
Eco91I GGTNACC 1 cut(s) 153
EcoO65I GGTNACC 1 cut(s) 153
EcoRI GAATTC 1 cut(s) 1365
EcoRII CCWGG 3 cut(s) 606, 995, 1165
EcoRV GATATC 1 cut(s) 1757
EcoT14I CCWWGG 3 cut(s) 131, 185, 1652
EcoT22I ATGCAT 2 cut(s) 31, 1048
EcoT38I GRGCYC 3 cut(s) 559, 1587, 1611
ErhI CCWWGG 3 cut(s) 131, 185, 1652
Esp3I CGTCTC 2 cut(s) 379, 966
FaeI CATG 6 cut(s) 29, 135, 189, 1046, 1050, 1215
FaqI GGGAC 8 cut(s) 132, 422, 482, 743, 803, 1339, 1391, 1577
FatI CATG 6 cut(s) 25, 131, 185, 1042, 1046, 1211
FauNDI CATATG 1 cut(s) 1019
FblI GTMKAC 1 cut(s) 942
FokI GGATG 4 cut(s) 73, 632, 1067, 1286
FriOI GRGCYC 3 cut(s) 559, 1587, 1611
FspBI CTAG 4 cut(s) 56, 581, 1284, 1371
FspI TGCGCA 1 cut(s) 382
HaeIII GGCC 7 cut(s) 458, 779, 1216, 1311, 1334, 1569, 1577
HgaI GACGC 7 cut(s) 368, 918, 979, 1488, 1579, 1630, 1701
Hin1I GRCGYC 6 cut(s) 971, 977, 1503, 1560, 1590, 1712
Hin1II CATG 6 cut(s) 29, 135, 189, 1046, 1050, 1215
HincII GTYRAC 3 cut(s) 943, 1258, 1399
HindII GTYRAC 3 cut(s) 943, 1258, 1399
HinfI GANTC 6 cut(s) 369, 634, 1069, 1193, 1382, 1741
HpaI GTTAAC 1 cut(s) 1258
HphI GGTGA 2 cut(s) 147, 1469
Hpy166II GTNNAC 5 cut(s) 943, 1258, 1399, 1421, 1517
Hpy188I TCNGA 7 cut(s) 304, 571, 881, 889, 1469, 1536, 1558
Hpy188III TCNNGA 5 cut(s) 70, 366, 539, 1190, 1507
Hpy8I GTNNAC 5 cut(s) 943, 1258, 1399, 1421, 1517
HpyAV CCTTC 5 cut(s) 410, 714, 731, 1149, 1588
HpyCH4III ACNGT 6 cut(s) 147, 335, 577, 921, 947, 1697
HpyCH4IV ACGT 8 cut(s) 83, 422, 743, 977, 1404, 1503, 1560, 1783
HpyCH4V TGCA 6 cut(s) 29, 45, 846, 1046, 1211, 1343
HpyF3I CTNAG 3 cut(s) 1222, 1271, 1319
HpySE526I ACGT 8 cut(s) 83, 422, 743, 977, 1404, 1503, 1560, 1783
Hsp92I GRCGYC 6 cut(s) 971, 977, 1503, 1560, 1590, 1712
Hsp92II CATG 6 cut(s) 29, 135, 189, 1046, 1050, 1215
KroI GCCGGC 7 cut(s) 454, 463, 640, 775, 784, 1075, 1569
KroNI GCCGGC 7 cut(s) 456, 465, 642, 777, 786, 1077, 1571
KspAI GTTAAC 1 cut(s) 1258
KspI CCGCGG 1 cut(s) 1309
Kzo9I GATC 6 cut(s) 566, 611, 881, 889, 1002, 1469
LmnI GCTCC 8 cut(s) 27, 318, 1174, 1232, 1440, 1606, 1618, 1682
Lsp1109I GCAGC 6 cut(s) 50, 398, 716, 719, 918, 1480
LweI GCATC 4 cut(s) 335, 371, 589, 1779
MabI ACCWGGT 1 cut(s) 995
MaeI CTAG 4 cut(s) 56, 581, 1284, 1371
MaeII ACGT 8 cut(s) 83, 422, 743, 977, 1404, 1503, 1560, 1783
MaeIII GTNAC 3 cut(s) 153, 622, 1057
MalI GATC 6 cut(s) 568, 613, 883, 891, 1004, 1471
MbiI CCGCTC 1 cut(s) 1613
MboI GATC 6 cut(s) 566, 611, 881, 889, 1002, 1469
MboII GAAGA 5 cut(s) 573, 994, 1346, 1713, 1790
MfeI CAATTG 1 cut(s) 1344
MhlI GDGCHC 7 cut(s) 24, 493, 559, 814, 1162, 1587, 1611
MlsI TGGCCA 1 cut(s) 1334
MluCI AATT 8 cut(s) 234, 256, 289, 711, 1146, 1249, 1344, 1365
MluI ACGCGT 1 cut(s) 910
MluNI TGGCCA 1 cut(s) 1334
MlyI GAGTC 4 cut(s) 628, 1063, 1391, 1735
MmeI TCCRAC 2 cut(s) 327, 1581
Mox20I TGGCCA 1 cut(s) 1334
Mph1103I ATGCAT 2 cut(s) 31, 1048
MroNI GCCGGC 7 cut(s) 454, 463, 640, 775, 784, 1075, 1569
MroXI GAANNNNTTC 1 cut(s) 1552
MscI TGGCCA 1 cut(s) 1334
MseI TTAA 6 cut(s) 534, 705, 1037, 1140, 1248, 1257
MslI CAYNNNNRTG 2 cut(s) 184, 1047
Msp20I TGGCCA 1 cut(s) 1334
MspA1I CMGCKG 2 cut(s) 931, 1308
MunI CAATTG 1 cut(s) 1344
Mva1269I GAATGC 1 cut(s) 994
MvaI CCWGG 3 cut(s) 608, 997, 1167
NaeI GCCGGC 7 cut(s) 456, 465, 642, 777, 786, 1077, 1571
NciI CCSGG 9 cut(s) 500, 676, 821, 1111, 1603, 1604, 1617, 1618, 1689
NcoI CCATGG 2 cut(s) 131, 185
NdeI CATATG 1 cut(s) 1019
NdeII GATC 6 cut(s) 566, 611, 881, 889, 1002, 1469
NgoMIV GCCGGC 7 cut(s) 454, 463, 640, 775, 784, 1075, 1569
NlaIII CATG 6 cut(s) 29, 135, 189, 1046, 1050, 1215
NmeAIII GCCGAG 3 cut(s) 1566, 1725, 1740
NmuCI GTSAC 1 cut(s) 153
NruI TCGCGA 1 cut(s) 1508
NsbI TGCGCA 1 cut(s) 382
NsiI ATGCAT 2 cut(s) 31, 1048
NspI RCATGY 1 cut(s) 1050
NspV TTCGAA 1 cut(s) 218
PauI GCGCGC 1 cut(s) 1673
PcsI WCGNNNNNNNCGW 7 cut(s) 885, 909, 913, 977, 1439, 1497, 1587
PctI GAATGC 1 cut(s) 994
PdiI GCCGGC 7 cut(s) 456, 465, 642, 777, 786, 1077, 1571
PdmI GAANNNNTTC 1 cut(s) 1552
PfeI GAWTC 2 cut(s) 369, 1193
Pfl23II CGTACG 1 cut(s) 913
PflFI GACNNNGTC 1 cut(s) 1403
PfoI TCCNGGA 2 cut(s) 498, 819
Ple19I CGATCG 1 cut(s) 892
PleI GAGTC 4 cut(s) 628, 1063, 1390, 1735
PmaCI CACGTG 1 cut(s) 84
PmlI CACGTG 1 cut(s) 84
PpsI GAGTC 4 cut(s) 628, 1063, 1390, 1735
Ppu21I YACGTR 1 cut(s) 84
PshBI ATTAAT 1 cut(s) 534
Psp1406I AACGTT 2 cut(s) 422, 743
Psp6I CCWGG 3 cut(s) 606, 995, 1165
PspCI CACGTG 1 cut(s) 84
PspEI GGTNACC 1 cut(s) 153
PspGI CCWGG 3 cut(s) 606, 995, 1165
PspLI CGTACG 1 cut(s) 913
PspPI GGNCC 4 cut(s) 854, 1452, 1567, 1575
PsyI GACNNNGTC 1 cut(s) 1403
PteI GCGCGC 1 cut(s) 1673
PvuI CGATCG 1 cut(s) 892
RruI TCGCGA 1 cut(s) 1508
RsaI GTAC 4 cut(s) 345, 915, 1420, 1624
RsaNI GTAC 4 cut(s) 344, 914, 1419, 1623
RseI CAYNNNNRTG 2 cut(s) 184, 1047
SacII CCGCGG 1 cut(s) 1309
SalI GTCGAC 1 cut(s) 941
SaqAI TTAA 6 cut(s) 534, 705, 1037, 1140, 1248, 1257
Sau3AI GATC 6 cut(s) 566, 611, 881, 889, 1002, 1469
Sau96I GGNCC 4 cut(s) 854, 1452, 1567, 1575
SchI GAGTC 4 cut(s) 628, 1063, 1391, 1735
SduI GDGCHC 7 cut(s) 24, 493, 559, 814, 1162, 1587, 1611
SexAI ACCWGGT 1 cut(s) 995
SfaNI GCATC 4 cut(s) 335, 371, 589, 1779
Sfr303I CCGCGG 1 cut(s) 1309
SfuI TTCGAA 1 cut(s) 218
SgrAI CRCCGGYG 1 cut(s) 1635
SgrBI CCGCGG 1 cut(s) 1309
SinI GGWCC 2 cut(s) 854, 1452
SmaI CCCGGG 2 cut(s) 1604, 1618
SmiMI CAYNNNNRTG 2 cut(s) 184, 1047
Sse9I AATT 8 cut(s) 234, 256, 289, 711, 1146, 1249, 1344, 1365
SspMI CTAG 4 cut(s) 56, 581, 1284, 1371
StyI CCWWGG 3 cut(s) 131, 185, 1652
TaaI ACNGT 6 cut(s) 147, 335, 577, 921, 947, 1697
TaiI ACGT 8 cut(s) 86, 425, 746, 980, 1407, 1506, 1563, 1786
TaqII GACCGA 1 cut(s) 842
TasI AATT 8 cut(s) 234, 256, 289, 711, 1146, 1249, 1344, 1365
TatI WGTACW 1 cut(s) 1418
TfiI GAWTC 2 cut(s) 369, 1193
Tru1I TTAA 6 cut(s) 534, 705, 1037, 1140, 1248, 1257
Tru9I TTAA 6 cut(s) 534, 705, 1037, 1140, 1248, 1257
TscAI CASTG 1 cut(s) 1524
TseFI GTSAC 1 cut(s) 153
TseI GCWGC 6 cut(s) 38, 411, 729, 732, 931, 1493
Tsp45I GTSAC 1 cut(s) 153
TspDTI ATGAA 4 cut(s) 233, 343, 520, 1791
TspGWI ACGGA 7 cut(s) 305, 732, 856, 948, 1167, 1243, 1767
TspMI CCCGGG 2 cut(s) 1602, 1616
TspRI CASTG 1 cut(s) 1524
Tth111I GACNNNGTC 1 cut(s) 1403
VpaK11BI GGWCC 2 cut(s) 854, 1452
VspI ATTAAT 1 cut(s) 534
XapI RAATTY 3 cut(s) 234, 256, 1365
XceI RCATGY 1 cut(s) 1050
XmaI CCCGGG 2 cut(s) 1602, 1616
XmiI GTMKAC 1 cut(s) 942
XmnI GAANNNNTTC 1 cut(s) 1552
XspI CTAG 4 cut(s) 56, 581, 1284, 1371
ZraI GACGTC 3 cut(s) 978, 1504, 1561
Zsp2I ATGCAT 2 cut(s) 31, 1048
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.