pycom2575g00010

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00002575
OG0002208
Physical Location & Seq
Reverse (-)
7047 .. 8600
1554 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom2575g00010.2

Sequence Viewer

Length: 981 bp
ATGAGTGGTAATTGGGTTGTATTCGACGGCAGGCTCCGTGCTTCGGCATCGAACTGTCGGCTCGTACCCCGCTTCATCGACGCGTCGAGATTCGTCTCGTGCGCAGGATGGGTTTCTGTTTTGGCTACCTGCTGCGAAGGAACGTTGCCTCTCGTCGTCCCTTTCCTCCTCCTGCCGGCCTCGCCGGCGGGAGGACGACATCGTGGCGGTGCTCGTGTCCCGGACGAGCCGCACTCGTTGCGGTGCAGTTTCGGTCCTCGGGTTTCCGTTCGACATCTCGGATGCGGAACGCTGAAGGGGCGGTGGGTCTTCACGACCTCCGATCGTCCGATCGAAGCCTTGTCGCTCGACGCGTACGACCGTCGCGCCCGCTGCGAGCCGTCGACCGTCAAGTTTGTTGATGGTATCTGCTACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAACAAACCCCGACTTCTGGAAGGGACGCATTTATTAGATAAAAGGTCGACGCGGGCTCTGCCCGTTGCTCTGATGATTCATGATAACTCGACGGATCGCACAGCCATCGTGCTGTTGGACCTTGGGTGGGTCGACCGGTCCGCCTCTGGTGTGCACCGGTCGGCTCGTCCCTTCTGCCGGCGATACGCTCCTGGCCTTAATTGGCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGAATACATTAGCATGGGATAACATCATAGGATTCGGTCCTATTCTGTTGGCCTCGGGATCGGAACGAACAACTGCGAAAGCATTGCCAAGGATGTTTCATAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGGCGGATGTTACTTTAGGACTCCGCCGGCACCTTATGAGAAATCAAAGTTTTGGGCCGGGGGGAGTATGGTCGCAAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

327

Amino Acids

35.67

Weight (kDa)

10.21

Isoelectric Point (pI)

50.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 103
Acc36I ACCTGC 1 cut(s) 137
AccB1I GGYRCC 1 cut(s) 927
AccI GTMKAC 3 cut(s) 383, 497, 582
AccII CGCG 4 cut(s) 83, 353, 366, 502
AclI AACGTT 1 cut(s) 143
AclWI GGATC 3 cut(s) 552, 784, 904
AcoI YGGCCR 1 cut(s) 653
AcuI CTGAAG 1 cut(s) 314
AfaI GTAC 2 cut(s) 66, 356
AfiI CCNNNNNNNGG 7 cut(s) 43, 175, 191, 466, 577, 627, 898
AflIII ACRYGT 2 cut(s) 81, 351
AgeI ACCGGT 2 cut(s) 585, 606
AgsI TTSAA 1 cut(s) 685
AjnI CCWGG 2 cut(s) 640, 891
AluBI AGCT 1 cut(s) 440
AluI AGCT 1 cut(s) 440
Alw21I GWGCWC 3 cut(s) 214, 606, 701
Alw26I GTCTC 2 cut(s) 100, 874
Alw44I GTGCAC 1 cut(s) 602
AlwI GGATC 3 cut(s) 552, 784, 904
Ama87I CYCGRG 2 cut(s) 258, 772
AoxI GGCC 5 cut(s) 177, 643, 653, 768, 953
ApaLI GTGCAC 1 cut(s) 602
ApeKI GCWGC 2 cut(s) 132, 372
AsiGI ACCGGT 2 cut(s) 585, 606
AspLEI GCGC 3 cut(s) 104, 368, 674
AspS9I GGNCC 5 cut(s) 254, 568, 588, 755, 953
AsuC2I CCSGG 3 cut(s) 221, 657, 957
AvaI CYCGRG 2 cut(s) 258, 772
AvaII GGWCC 4 cut(s) 254, 568, 588, 755
BaeGI GKGCMC 1 cut(s) 606
BanI GGYRCC 1 cut(s) 927
BanII GRGCYC 2 cut(s) 508, 844
BauI CACGAG 2 cut(s) 97, 213
BbsI GAAGAC 2 cut(s) 301, 853
Bbv12I GWGCWC 3 cut(s) 214, 606, 701
BbvI GCAGC 2 cut(s) 119, 359
BccI CCATC 3 cut(s) 102, 395, 563
BceAI ACGGC 2 cut(s) 43, 364
BcgI CGANNNNNNTGC 4 cut(s) 538, 572, 784, 818
BciT130I CCWGG 2 cut(s) 642, 893
BcnI CCSGG 3 cut(s) 221, 657, 957
BcoDI GTCTC 2 cut(s) 100, 874
BfaI CTAG 2 cut(s) 435, 866
BfoI RGCGCY 1 cut(s) 675
BfuAI ACCTGC 1 cut(s) 137
BglI GCCNNNNNGGC 1 cut(s) 185
BisI GCNGC 3 cut(s) 133, 230, 373
BlsI GCNGC 3 cut(s) 134, 231, 374
Bme1390I CCNGG 5 cut(s) 221, 642, 657, 893, 957
Bme18I GGWCC 4 cut(s) 254, 568, 588, 755
BmeT110I CYCGRG 2 cut(s) 258, 772
BmgT120I GGNCC 5 cut(s) 254, 568, 588, 755, 953
BmiI GGNNCC 2 cut(s) 35, 929
BmrFI CCNGG 5 cut(s) 221, 642, 657, 893, 957
BmsI GCATC 3 cut(s) 56, 272, 874
BpiI GAAGAC 2 cut(s) 301, 853
BplI GAGNNNNNCTC 2 cut(s) 218, 250
BpuMI CCSGG 3 cut(s) 221, 657, 957
BsaAI YACGTR 1 cut(s) 447
BsaJI CCNNGG 6 cut(s) 257, 571, 771, 806, 892, 956
BsaWI WCCGGW 2 cut(s) 585, 606
Bsc4I CCNNNNNNNGG 7 cut(s) 43, 175, 191, 466, 577, 627, 898
Bse118I RCCGGY 6 cut(s) 175, 184, 585, 606, 627, 924
Bse3DI GCAATG 1 cut(s) 800
BseBI CCWGG 2 cut(s) 642, 893
BseDI CCNNGG 6 cut(s) 257, 571, 771, 806, 892, 956
BseGI GGATG 4 cut(s) 113, 287, 816, 910
BseLI CCNNNNNNNGG 7 cut(s) 43, 175, 191, 466, 577, 627, 898
BseMI GCAATG 1 cut(s) 800
BseRI GAGGAG 1 cut(s) 158
BseSI GKGCMC 1 cut(s) 606
BseXI GCAGC 2 cut(s) 119, 359
BsgI GTGCAG 1 cut(s) 265
Bsh1236I CGCG 4 cut(s) 83, 353, 366, 502
Bsh1285I CGRYCG 6 cut(s) 325, 333, 361, 387, 586, 611
BshFI GGCC 5 cut(s) 179, 645, 655, 770, 955
BshNI GGYRCC 1 cut(s) 927
BshTI ACCGGT 2 cut(s) 585, 606
BsiEI CGRYCG 6 cut(s) 325, 333, 361, 387, 586, 611
BsiHKAI GWGCWC 3 cut(s) 214, 606, 701
BsiHKCI CYCGRG 2 cut(s) 258, 772
BsiWI CGTACG 1 cut(s) 354
BslFI GGGAC 4 cut(s) 143, 203, 487, 603
BslI CCNNNNNNNGG 7 cut(s) 43, 175, 191, 466, 577, 627, 898
BsmAI GTCTC 2 cut(s) 100, 874
BsmBI CGTCTC 1 cut(s) 100
BsmFI GGGAC 4 cut(s) 143, 203, 487, 603
BsnI GGCC 5 cut(s) 179, 645, 655, 770, 955
BsoBI CYCGRG 2 cut(s) 258, 772
Bsp1286I GDGCHC 5 cut(s) 214, 508, 606, 701, 844
Bsp143I GATC 6 cut(s) 322, 330, 544, 776, 851, 896
BspANI GGCC 5 cut(s) 179, 645, 655, 770, 955
BspFNI CGCG 4 cut(s) 83, 353, 366, 502
BspHI TCATGA 1 cut(s) 529
BspLI GGNNCC 2 cut(s) 35, 929
BspMI ACCTGC 1 cut(s) 137
BspPI GGATC 3 cut(s) 552, 784, 904
BspT107I GGYRCC 1 cut(s) 927
BsrDI GCAATG 1 cut(s) 800
BsrFI RCCGGY 6 cut(s) 175, 184, 585, 606, 627, 924
BssAI RCCGGY 6 cut(s) 175, 184, 585, 606, 627, 924
BssECI CCNNGG 6 cut(s) 257, 571, 771, 806, 892, 956
BssMI GATC 6 cut(s) 322, 330, 544, 776, 851, 896
BssSI CACGAG 2 cut(s) 97, 213
BssT1I CCWWGG 2 cut(s) 571, 806
Bst2BI CACGAG 2 cut(s) 97, 213
Bst2UI CCWGG 2 cut(s) 642, 893
Bst4CI ACNGT 5 cut(s) 56, 362, 388, 425, 862
BstAPI GCANNNNNTGC 1 cut(s) 238
BstBAI YACGTR 1 cut(s) 447
BstC8I GCNNGC 9 cut(s) 32, 177, 186, 370, 377, 504, 629, 708, 926
BstF5I GGATG 4 cut(s) 113, 287, 816, 910
BstFNI CGCG 4 cut(s) 83, 353, 366, 502
BstH2I RGCGCY 1 cut(s) 675
BstHHI GCGC 3 cut(s) 104, 368, 674
BstKTI GATC 6 cut(s) 325, 333, 547, 779, 854, 899
BstMAI GTCTC 2 cut(s) 100, 874
BstMBI GATC 6 cut(s) 322, 330, 544, 776, 851, 896
BstMCI CGRYCG 6 cut(s) 325, 333, 361, 387, 586, 611
BstMWI GCNNNNNNNGC 7 cut(s) 181, 185, 238, 298, 372, 508, 671
BstNI CCWGG 2 cut(s) 642, 893
BstSCI CCNGG 5 cut(s) 219, 640, 655, 891, 955
BstSLI GKGCMC 1 cut(s) 606
BstUI CGCG 4 cut(s) 83, 353, 366, 502
BstV1I GCAGC 2 cut(s) 119, 359
BstV2I GAAGAC 2 cut(s) 301, 853
BsuRI GGCC 5 cut(s) 179, 645, 655, 770, 955
BtsCI GGATG 4 cut(s) 113, 287, 816, 910
BveI ACCTGC 1 cut(s) 137
Cac8I GCNNGC 9 cut(s) 32, 177, 186, 370, 377, 504, 629, 708, 926
CciI TCATGA 1 cut(s) 529
CfoI GCGC 3 cut(s) 104, 368, 674
Cfr10I RCCGGY 6 cut(s) 175, 184, 585, 606, 627, 924
Cfr13I GGNCC 5 cut(s) 254, 568, 588, 755, 953
CpoI CGGWCCG 1 cut(s) 588
CseI GACGC 5 cut(s) 72, 89, 359, 484, 508
Csp6I GTAC 2 cut(s) 65, 355
CspAI ACCGGT 2 cut(s) 585, 606
CspI CGGWCCG 1 cut(s) 588
CviAII CATG 2 cut(s) 530, 732
CviQI GTAC 2 cut(s) 65, 355
DpnI GATC 6 cut(s) 324, 332, 546, 778, 853, 898
DpnII GATC 6 cut(s) 322, 330, 544, 776, 851, 896
EaeI YGGCCR 1 cut(s) 653
EciI GGCGGA 3 cut(s) 580, 911, 917
Eco130I CCWWGG 2 cut(s) 571, 806
Eco24I GRGCYC 2 cut(s) 508, 844
Eco47I GGWCC 4 cut(s) 254, 568, 588, 755
Eco57I CTGAAG 1 cut(s) 314
Eco88I CYCGRG 2 cut(s) 258, 772
EcoRII CCWGG 2 cut(s) 640, 891
EcoT14I CCWWGG 2 cut(s) 571, 806
EcoT38I GRGCYC 2 cut(s) 508, 844
ErhI CCWWGG 2 cut(s) 571, 806
Esp3I CGTCTC 1 cut(s) 100
FaeI CATG 2 cut(s) 533, 735
FaiI YATR 7 cut(s) 531, 733, 746, 819, 878, 935, 967
FaqI GGGAC 4 cut(s) 143, 203, 487, 603
FatI CATG 2 cut(s) 529, 731
FauI CCCGC 4 cut(s) 77, 181, 377, 495
FblI GTMKAC 3 cut(s) 383, 497, 582
Fnu4HI GCNGC 3 cut(s) 133, 230, 373
FokI GGATG 4 cut(s) 120, 294, 823, 917
FriOI GRGCYC 2 cut(s) 508, 844
Fsp4HI GCNGC 3 cut(s) 133, 230, 373
FspBI CTAG 2 cut(s) 435, 866
FspI TGCGCA 1 cut(s) 103
GlaI GCGC 3 cut(s) 103, 367, 673
GluI GCNGC 3 cut(s) 133, 230, 373
HaeII RGCGCY 1 cut(s) 675
HaeIII GGCC 5 cut(s) 179, 645, 655, 770, 955
HgaI GACGC 5 cut(s) 72, 89, 359, 484, 508
HhaI GCGC 3 cut(s) 104, 368, 674
Hin1II CATG 2 cut(s) 533, 735
Hin6I GCGC 3 cut(s) 102, 366, 672
HinP1I GCGC 3 cut(s) 102, 366, 672
HincII GTYRAC 3 cut(s) 384, 498, 583
HindII GTYRAC 3 cut(s) 384, 498, 583
HinfI GANTC 4 cut(s) 90, 526, 750, 918
Hpy166II GTNNAC 4 cut(s) 384, 498, 583, 604
Hpy188I TCNGA 8 cut(s) 281, 322, 330, 418, 522, 720, 781, 856
Hpy188III TCNNGA 7 cut(s) 87, 313, 435, 467, 530, 774, 824
Hpy8I GTNNAC 4 cut(s) 384, 498, 583, 604
Hpy99I CGWCG 9 cut(s) 29, 83, 88, 158, 353, 366, 385, 502, 544
HpyAV CCTTC 4 cut(s) 131, 289, 464, 631
HpyCH4III ACNGT 5 cut(s) 56, 362, 388, 425, 862
HpyCH4IV ACGT 2 cut(s) 143, 446
HpyCH4V TGCA 3 cut(s) 246, 450, 604
HpyF10VI GCNNNNNNNGC 7 cut(s) 181, 185, 238, 298, 372, 508, 671
HpySE526I ACGT 2 cut(s) 143, 446
Hsp92II CATG 2 cut(s) 533, 735
HspAI GCGC 3 cut(s) 102, 366, 672
KroI GCCGGC 4 cut(s) 175, 184, 627, 924
KroNI GCCGGC 4 cut(s) 177, 186, 629, 926
Kzo9I GATC 6 cut(s) 322, 330, 544, 776, 851, 896
LmnI GCTCC 2 cut(s) 39, 643
Lsp1109I GCAGC 2 cut(s) 119, 359
LweI GCATC 3 cut(s) 56, 272, 874
MaeI CTAG 2 cut(s) 435, 866
MaeII ACGT 2 cut(s) 143, 446
MaeIII GTNAC 2 cut(s) 676, 907
MalI GATC 6 cut(s) 324, 332, 546, 778, 853, 898
MboI GATC 6 cut(s) 322, 330, 544, 776, 851, 896
MboII GAAGA 3 cut(s) 301, 697, 858
MhlI GDGCHC 5 cut(s) 214, 508, 606, 701, 844
MluCI AATT 4 cut(s) 10, 430, 649, 689
MluI ACGCGT 2 cut(s) 81, 351
MlyI GAGTC 1 cut(s) 912
MmeI TCCRAC 1 cut(s) 546
MreI CGCCGGCG 1 cut(s) 184
MroNI GCCGGC 4 cut(s) 175, 184, 627, 924
MseI TTAA 1 cut(s) 648
MslI CAYNNNNRTG 1 cut(s) 730
MspA1I CMGCKG 1 cut(s) 372
MspR9I CCNGG 5 cut(s) 221, 642, 657, 893, 957
MvaI CCWGG 2 cut(s) 642, 893
MvnI CGCG 4 cut(s) 83, 353, 366, 502
MwoI GCNNNNNNNGC 7 cut(s) 181, 185, 238, 298, 372, 508, 671
NaeI GCCGGC 4 cut(s) 177, 186, 629, 926
NciI CCSGG 3 cut(s) 221, 657, 957
NdeII GATC 6 cut(s) 322, 330, 544, 776, 851, 896
NgoMIV GCCGGC 4 cut(s) 175, 184, 627, 924
NlaIII CATG 2 cut(s) 533, 735
NlaIV GGNNCC 2 cut(s) 35, 929
NsbI TGCGCA 1 cut(s) 103
PagI TCATGA 1 cut(s) 529
PcsI WCGNNNNNNNCGW 2 cut(s) 350, 354
PdiI GCCGGC 4 cut(s) 177, 186, 629, 926
PfeI GAWTC 3 cut(s) 90, 526, 750
Pfl23II CGTACG 1 cut(s) 354
PfoI TCCNGGA 1 cut(s) 219
PinAI ACCGGT 2 cut(s) 585, 606
PkrI GCNGC 3 cut(s) 134, 231, 374
Ple19I CGATCG 2 cut(s) 325, 333
PleI GAGTC 1 cut(s) 912
PpsI GAGTC 1 cut(s) 912
Ppu21I YACGTR 1 cut(s) 447
Psp1406I AACGTT 1 cut(s) 143
Psp6I CCWGG 2 cut(s) 640, 891
PspGI CCWGG 2 cut(s) 640, 891
PspLI CGTACG 1 cut(s) 354
PspN4I GGNNCC 2 cut(s) 35, 929
PspPI GGNCC 5 cut(s) 254, 568, 588, 755, 953
PvuI CGATCG 2 cut(s) 325, 333
RsaI GTAC 2 cut(s) 66, 356
RsaNI GTAC 2 cut(s) 65, 355
RseI CAYNNNNRTG 1 cut(s) 730
Rsr2I CGGWCCG 1 cut(s) 588
RsrII CGGWCCG 1 cut(s) 588
SalI GTCGAC 3 cut(s) 382, 496, 581
SaqAI TTAA 1 cut(s) 648
SatI GCNGC 3 cut(s) 133, 230, 373
Sau3AI GATC 6 cut(s) 322, 330, 544, 776, 851, 896
Sau96I GGNCC 5 cut(s) 254, 568, 588, 755, 953
SchI GAGTC 1 cut(s) 912
ScrFI CCNGG 5 cut(s) 221, 642, 657, 893, 957
SduI GDGCHC 5 cut(s) 214, 508, 606, 701, 844
SetI ASST 8 cut(s) 131, 146, 320, 442, 449, 497, 573, 933
SfaNI GCATC 3 cut(s) 56, 272, 874
SgrAI CRCCGGYG 1 cut(s) 184
SinI GGWCC 4 cut(s) 254, 568, 588, 755
SmiMI CAYNNNNRTG 1 cut(s) 730
Sse9I AATT 4 cut(s) 10, 430, 649, 689
SspMI CTAG 2 cut(s) 435, 866
StyD4I CCNGG 5 cut(s) 219, 640, 655, 891, 955
StyI CCWWGG 2 cut(s) 571, 806
TaaI ACNGT 5 cut(s) 56, 362, 388, 425, 862
TaiI ACGT 2 cut(s) 146, 449
TaqII GACCGA 2 cut(s) 242, 743
TasI AATT 4 cut(s) 10, 430, 649, 689
TauI GCSGC 1 cut(s) 232
TfiI GAWTC 3 cut(s) 90, 526, 750
Tru1I TTAA 1 cut(s) 648
Tru9I TTAA 1 cut(s) 648
TseI GCWGC 2 cut(s) 132, 372
TspDTI ATGAA 3 cut(s) 64, 518, 806
TspGWI ACGGA 3 cut(s) 26, 256, 557
VneI GTGCAC 1 cut(s) 602
VpaK11BI GGWCC 4 cut(s) 254, 568, 588, 755
XbaI TCTAGA 1 cut(s) 434
XcmI CCANNNNNNNNNTGG 1 cut(s) 562
XmiI GTMKAC 3 cut(s) 383, 497, 582
XspI CTAG 2 cut(s) 435, 866
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.