pycom2303g00100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00002303
OG0002208
Physical Location & Seq
Reverse (-)
28858 .. 31616
2759 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom2303g00100.3

Sequence Viewer

Length: 1551 bp
ATGGGTTTCTGTTTTGGCTACCTGCTGCGAAGGAACGTTGCCTCTCGACGACATAGTGGCGGTGCTCGTGTCCCGGACGAGCCGCACTCGTTGCGGTGCAGTTTCGGTCCTCGGGTTTCCGTTCGACATCTCGGATGCGGAACGCTGAAGGGGCGGTGGGTCTTCACGACCTCCGATCGTCCGATCGAAGCCTTGTCGCTCGACGCGTACGACCGTCGCGCCCGCTGCGAGCCGTCGACCGTCAAGGTCGTCCGTCTCGCGCGACCTCGTAGTTGGACCTTGGGTTGGGTCGACCGGTCCGCCTCTGGTGTGCACCGGTCGGCTCGTCCCTTCTGCCGGCGATACGCTCCTGGCCTTAATTGGCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGAATACATTAGCATGGGATAACATCATAGGATTTCGACGAACAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGGCGGATGTTACTTTTAGGACTCCGCCGGCACCTTATGAGAAATCAAAGTTTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGATCATTGCAATTGTTGGTCTTCAACGAGGAATTCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTCCTACCGATTGAATGGTCCGGTGAAGTGTTCGGATCGCGGCGACGCGGGTGGTTCGCTGCCCGCGACGTCCAGAACGACCCGAGAACCCGTTCCGACGTCGGGGGCCGGCGGGCCTTCGGGCTCGGCGTCCCCTTATCCCGGGAGCCCGCTCCCGGGCGTACCAACGAACACCGGCGCGTCTTTGCGCCAAGGAACCCGAACGAAAGATCAAAACGACTCTCGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTGAATTGCAGAATCCCGTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAAGCCTTTAGGCCGAGGGCACGCCTGCCTGGGCGTCACACGCCGTTGCCCCCCCGCGCCTCCCTCGGGAGCGTCGGGGGGCGGACGATGGCCTCCCGTGCGTCATCCCGCGCGGTTGGCACAAATGCCGGGTCCTCGGCGACGAACGCCACGACGATCGGTGCGTTTCCCAACCTCGGTTGCATGTTGTGCGCCTTCGTCGCGCTCCGAGCGGCCCGCGACGCTCCCCGCTCTGCTTCGGCCGAGCTTTCAACGCGACCCCAGGTCAGGCGGGGTTACCCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

517

Amino Acids

56.78

Weight (kDa)

11.31

Isoelectric Point (pI)

65.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 1002, 1032
Acc36I ACCTGC 1 cut(s) 30
AccB1I GGYRCC 2 cut(s) 613, 694
AccBSI CCGCTC 3 cut(s) 1082, 1477, 1496
AccI GTMKAC 2 cut(s) 236, 291
AclI AACGTT 1 cut(s) 36
AclWI GGATC 2 cut(s) 589, 973
AcoI YGGCCR 3 cut(s) 362, 749, 1505
AcsI RAATTY 1 cut(s) 861
AcuI CTGAAG 1 cut(s) 167
AcyI GRCGYC 4 cut(s) 999, 1029, 1059, 1299
AfaI GTAC 3 cut(s) 209, 916, 1093
AflIII ACRYGT 1 cut(s) 204
AgeI ACCGGT 2 cut(s) 294, 315
AgsI TTSAA 5 cut(s) 394, 854, 944, 1250, 1517
AhdI GACNNNNNGTC 1 cut(s) 1528
AjnI CCWGG 5 cut(s) 349, 576, 700, 1293, 1526
AjuI GAANNNNNNNTTGG 2 cut(s) 482, 514
AluBI AGCT 3 cut(s) 822, 887, 1512
AluI AGCT 3 cut(s) 822, 887, 1512
Alw21I GWGCWC 3 cut(s) 67, 315, 410
Alw26I GTCTC 3 cut(s) 260, 559, 795
Alw44I GTGCAC 1 cut(s) 311
AlwI GGATC 2 cut(s) 589, 973
Ama87I CYCGRG 5 cut(s) 111, 1012, 1071, 1085, 1330
AoxI GGCC 9 cut(s) 352, 362, 749, 1036, 1044, 1276, 1355, 1478, 1505
ApaLI GTGCAC 1 cut(s) 311
ApeKI GCWGC 3 cut(s) 25, 225, 989
ApoI RAATTY 1 cut(s) 861
ArsI GACNNNNNNTTYG 2 cut(s) 1381, 1413
AseI ATTAAT 1 cut(s) 504
AsiGI ACCGGT 2 cut(s) 294, 315
Asp700I GAANNNNTTC 1 cut(s) 1021
AspS9I GGNCC 8 cut(s) 107, 276, 297, 948, 1036, 1044, 1397, 1479
AsuC2I CCSGG 8 cut(s) 74, 366, 646, 1072, 1073, 1086, 1087, 1395
AsuHPI GGTGA 1 cut(s) 965
AvaI CYCGRG 5 cut(s) 111, 1012, 1071, 1085, 1330
AvaII GGWCC 5 cut(s) 107, 276, 297, 948, 1397
BaeGI GKGCMC 3 cut(s) 315, 697, 1287
BanI GGYRCC 2 cut(s) 613, 694
BanII GRGCYC 3 cut(s) 529, 1056, 1080
BauI CACGAG 1 cut(s) 66
BbsI GAAGAC 3 cut(s) 154, 538, 842
Bbv12I GWGCWC 3 cut(s) 67, 315, 410
BbvCI CCTCAGC 1 cut(s) 806
BbvI GCAGC 3 cut(s) 12, 212, 976
BccI CCATC 2 cut(s) 1245, 1347
BceAI ACGGC 3 cut(s) 217, 736, 1293
BciT130I CCWGG 5 cut(s) 351, 578, 702, 1295, 1528
BcnI CCSGG 8 cut(s) 74, 366, 646, 1072, 1073, 1086, 1087, 1395
BcoDI GTCTC 3 cut(s) 260, 559, 795
BfaI CTAG 3 cut(s) 551, 819, 867
BfoI RGCGCY 1 cut(s) 384
BfuAI ACCTGC 1 cut(s) 30
BisI GCNGC 6 cut(s) 26, 83, 226, 971, 990, 1478
BlsI GCNGC 6 cut(s) 27, 84, 227, 972, 991, 1479
Bme18I GGWCC 5 cut(s) 107, 276, 297, 948, 1397
BmeRI GACNNNNNGTC 1 cut(s) 1528
BmeT110I CYCGRG 5 cut(s) 111, 1012, 1071, 1085, 1330
BmgT120I GGNCC 8 cut(s) 107, 276, 297, 948, 1036, 1044, 1397, 1479
BmiI GGNNCC 8 cut(s) 615, 643, 696, 711, 1037, 1077, 1127, 1398
BmsI GCATC 3 cut(s) 125, 559, 1187
BpiI GAAGAC 3 cut(s) 154, 538, 842
BplI GAGNNNNNCTC 2 cut(s) 71, 103
Bpu10I CCTNAGC 1 cut(s) 806
BpuMI CCSGG 8 cut(s) 74, 366, 646, 1072, 1073, 1086, 1087, 1395
Bsa29I ATCGAT 1 cut(s) 1181
BsaHI GRCGYC 4 cut(s) 999, 1029, 1059, 1299
BsaI GGTCTC 1 cut(s) 795
BsaWI WCCGGW 3 cut(s) 294, 315, 950
Bse118I RCCGGY 6 cut(s) 294, 315, 336, 610, 1038, 1104
Bse3DI GCAATG 1 cut(s) 834
BseBI CCWGG 5 cut(s) 351, 578, 702, 1295, 1528
BseCI ATCGAT 1 cut(s) 1181
BseGI GGATG 4 cut(s) 140, 499, 595, 1369
BseMI GCAATG 1 cut(s) 834
BseMII CTCAG 1 cut(s) 820
BseSI GKGCMC 3 cut(s) 315, 697, 1287
BseX3I CGGCCG 1 cut(s) 1505
BseXI GCAGC 3 cut(s) 12, 212, 976
BsgI GTGCAG 1 cut(s) 118
Bsh1285I CGRYCG 8 cut(s) 178, 186, 214, 240, 295, 320, 1424, 1508
BshFI GGCC 9 cut(s) 354, 364, 751, 1038, 1046, 1278, 1357, 1480, 1507
BshNI GGYRCC 2 cut(s) 613, 694
BshTI ACCGGT 2 cut(s) 294, 315
BshVI ATCGAT 1 cut(s) 1181
BsiEI CGRYCG 8 cut(s) 178, 186, 214, 240, 295, 320, 1424, 1508
BsiHKAI GWGCWC 3 cut(s) 67, 315, 410
BsiHKCI CYCGRG 5 cut(s) 111, 1012, 1071, 1085, 1330
BsiWI CGTACG 1 cut(s) 207
BslFI GGGAC 4 cut(s) 56, 312, 887, 1046
BsmAI GTCTC 3 cut(s) 260, 559, 795
BsmBI CGTCTC 1 cut(s) 260
BsmFI GGGAC 4 cut(s) 56, 312, 887, 1046
BsnI GGCC 9 cut(s) 354, 364, 751, 1038, 1046, 1278, 1357, 1480, 1507
Bso31I GGTCTC 1 cut(s) 795
BsoBI CYCGRG 5 cut(s) 111, 1012, 1071, 1085, 1330
Bsp1286I GDGCHC 8 cut(s) 67, 315, 410, 529, 697, 1056, 1080, 1287
Bsp1407I TGTACA 1 cut(s) 914
Bsp143I GATC 8 cut(s) 175, 183, 536, 581, 831, 965, 1139, 1421
BspANI GGCC 9 cut(s) 354, 364, 751, 1038, 1046, 1278, 1357, 1480, 1507
BspCNI CTCAG 1 cut(s) 819
BspDI ATCGAT 1 cut(s) 1181
BspLI GGNNCC 8 cut(s) 615, 643, 696, 711, 1037, 1077, 1127, 1398
BspMI ACCTGC 1 cut(s) 30
BspPI GGATC 2 cut(s) 589, 973
BspT107I GGYRCC 2 cut(s) 613, 694
BspTNI GGTCTC 1 cut(s) 795
BsrBI CCGCTC 3 cut(s) 1082, 1477, 1496
BsrDI GCAATG 1 cut(s) 834
BsrFI RCCGGY 6 cut(s) 294, 315, 336, 610, 1038, 1104
BsrGI TGTACA 1 cut(s) 914
BssAI RCCGGY 6 cut(s) 294, 315, 336, 610, 1038, 1104
BssMI GATC 8 cut(s) 175, 183, 536, 581, 831, 965, 1139, 1421
BssNI GRCGYC 4 cut(s) 999, 1029, 1059, 1299
BssSI CACGAG 1 cut(s) 66
BssT1I CCWWGG 3 cut(s) 279, 489, 1121
Bst2BI CACGAG 1 cut(s) 66
Bst2UI CCWGG 5 cut(s) 351, 578, 702, 1295, 1528
Bst4CI ACNGT 3 cut(s) 215, 241, 547
BstACI GRCGYC 4 cut(s) 999, 1029, 1059, 1299
BstAPI GCANNNNNTGC 2 cut(s) 91, 1454
BstAUI TGTACA 1 cut(s) 914
BstDEI CTNAG 2 cut(s) 757, 806
BstEII GGTNACC 1 cut(s) 1540
BstF5I GGATG 4 cut(s) 140, 499, 595, 1369
BstH2I RGCGCY 1 cut(s) 384
BstKTI GATC 8 cut(s) 178, 186, 539, 584, 834, 968, 1142, 1424
BstMAI GTCTC 3 cut(s) 260, 559, 795
BstMBI GATC 8 cut(s) 175, 183, 536, 581, 831, 965, 1139, 1421
BstMCI CGRYCG 8 cut(s) 178, 186, 214, 240, 295, 320, 1424, 1508
BstNI CCWGG 5 cut(s) 351, 578, 702, 1295, 1528
BstNSI RCATGY 1 cut(s) 1452
BstPI GGTNACC 1 cut(s) 1540
BstSLI GKGCMC 3 cut(s) 315, 697, 1287
BstV1I GCAGC 3 cut(s) 12, 212, 976
BstV2I GAAGAC 3 cut(s) 154, 538, 842
BstZI CGGCCG 1 cut(s) 1505
Bsu15I ATCGAT 1 cut(s) 1181
BsuRI GGCC 9 cut(s) 354, 364, 751, 1038, 1046, 1278, 1357, 1480, 1507
BsuTUI ATCGAT 1 cut(s) 1181
BtsCI GGATG 4 cut(s) 140, 499, 595, 1369
BveI ACCTGC 1 cut(s) 30
Cfr10I RCCGGY 6 cut(s) 294, 315, 336, 610, 1038, 1104
Cfr13I GGNCC 8 cut(s) 107, 276, 297, 948, 1036, 1044, 1397, 1479
Cfr9I CCCGGG 2 cut(s) 1071, 1085
ClaI ATCGAT 1 cut(s) 1181
CpoI CGGWCCG 1 cut(s) 297
CseI GACGC 8 cut(s) 212, 984, 1048, 1099, 1288, 1326, 1355, 1495
Csp6I GTAC 3 cut(s) 208, 915, 1092
CspAI ACCGGT 2 cut(s) 294, 315
CspI CGGWCCG 1 cut(s) 297
CviAII CATG 3 cut(s) 441, 747, 1449
CviQI GTAC 3 cut(s) 208, 915, 1092
DdeI CTNAG 2 cut(s) 757, 806
DpnI GATC 8 cut(s) 177, 185, 538, 583, 833, 967, 1141, 1423
DpnII GATC 8 cut(s) 175, 183, 536, 581, 831, 965, 1139, 1421
DriI GACNNNNNGTC 1 cut(s) 1528
EaeI YGGCCR 3 cut(s) 362, 749, 1505
EagI CGGCCG 1 cut(s) 1505
Eam1105I GACNNNNNGTC 1 cut(s) 1528
EciI GGCGGA 4 cut(s) 289, 597, 602, 1362
EclXI CGGCCG 1 cut(s) 1505
Eco130I CCWWGG 3 cut(s) 279, 489, 1121
Eco24I GRGCYC 3 cut(s) 529, 1056, 1080
Eco31I GGTCTC 1 cut(s) 795
Eco32I GATATC 1 cut(s) 1165
Eco47I GGWCC 5 cut(s) 107, 276, 297, 948, 1397
Eco52I CGGCCG 1 cut(s) 1505
Eco57I CTGAAG 1 cut(s) 167
Eco88I CYCGRG 5 cut(s) 111, 1012, 1071, 1085, 1330
Eco91I GGTNACC 1 cut(s) 1540
EcoO109I RGGNCCY 1 cut(s) 1397
EcoO65I GGTNACC 1 cut(s) 1540
EcoRI GAATTC 1 cut(s) 861
EcoRII CCWGG 5 cut(s) 349, 576, 700, 1293, 1526
EcoRV GATATC 1 cut(s) 1165
EcoT14I CCWWGG 3 cut(s) 279, 489, 1121
EcoT38I GRGCYC 3 cut(s) 529, 1056, 1080
ErhI CCWWGG 3 cut(s) 279, 489, 1121
Esp3I CGTCTC 1 cut(s) 260
FaeI CATG 3 cut(s) 444, 750, 1452
FaqI GGGAC 4 cut(s) 56, 312, 887, 1046
FatI CATG 3 cut(s) 440, 746, 1448
FblI GTMKAC 2 cut(s) 236, 291
Fnu4HI GCNGC 6 cut(s) 26, 83, 226, 971, 990, 1478
FokI GGATG 4 cut(s) 147, 506, 602, 1356
FriOI GRGCYC 3 cut(s) 529, 1056, 1080
Fsp4HI GCNGC 6 cut(s) 26, 83, 226, 971, 990, 1478
FspBI CTAG 3 cut(s) 551, 819, 867
GluI GCNGC 6 cut(s) 26, 83, 226, 971, 990, 1478
HaeII RGCGCY 1 cut(s) 384
HaeIII GGCC 9 cut(s) 354, 364, 751, 1038, 1046, 1278, 1357, 1480, 1507
HgaI GACGC 8 cut(s) 212, 984, 1048, 1099, 1288, 1326, 1355, 1495
Hin1I GRCGYC 4 cut(s) 999, 1029, 1059, 1299
Hin1II CATG 3 cut(s) 444, 750, 1452
HincII GTYRAC 4 cut(s) 237, 292, 793, 895
HindII GTYRAC 4 cut(s) 237, 292, 793, 895
HinfI GANTC 6 cut(s) 604, 728, 878, 1149, 1225, 1242
HpaI GTTAAC 1 cut(s) 793
HphI GGTGA 1 cut(s) 965
Hpy166II GTNNAC 7 cut(s) 237, 292, 313, 793, 895, 917, 1234
Hpy188I TCNGA 8 cut(s) 134, 175, 183, 429, 541, 965, 1027, 1474
Hpy188III TCNNGA 6 cut(s) 45, 166, 509, 725, 1003, 1332
Hpy8I GTNNAC 7 cut(s) 237, 292, 313, 793, 895, 917, 1234
HpyAV CCTTC 6 cut(s) 24, 142, 340, 684, 1057, 1470
HpyCH4III ACNGT 3 cut(s) 215, 241, 547
HpyCH4IV ACGT 5 cut(s) 36, 900, 999, 1029, 1191
HpyCH4V TGCA 6 cut(s) 99, 313, 746, 839, 1222, 1448
HpyF3I CTNAG 2 cut(s) 757, 806
HpySE526I ACGT 5 cut(s) 36, 900, 999, 1029, 1191
Hsp92I GRCGYC 4 cut(s) 999, 1029, 1059, 1299
Hsp92II CATG 3 cut(s) 444, 750, 1452
KroI GCCGGC 3 cut(s) 336, 610, 1038
KroNI GCCGGC 3 cut(s) 338, 612, 1040
KspAI GTTAAC 1 cut(s) 793
Kzo9I GATC 8 cut(s) 175, 183, 536, 581, 831, 965, 1139, 1421
LmnI GCTCC 9 cut(s) 352, 709, 767, 936, 1075, 1087, 1334, 1475, 1494
Lsp1109I GCAGC 3 cut(s) 12, 212, 976
LweI GCATC 3 cut(s) 125, 559, 1187
MaeI CTAG 3 cut(s) 551, 819, 867
MaeII ACGT 5 cut(s) 36, 900, 999, 1029, 1191
MaeIII GTNAC 4 cut(s) 385, 592, 1300, 1540
MalI GATC 8 cut(s) 177, 185, 538, 583, 833, 967, 1141, 1423
MbiI CCGCTC 3 cut(s) 1082, 1477, 1496
MboI GATC 8 cut(s) 175, 183, 536, 581, 831, 965, 1139, 1421
MboII GAAGA 5 cut(s) 154, 406, 543, 842, 1198
MfeI CAATTG 1 cut(s) 840
MhlI GDGCHC 8 cut(s) 67, 315, 410, 529, 697, 1056, 1080, 1287
MluCI AATT 7 cut(s) 358, 398, 681, 784, 840, 861, 1217
MluI ACGCGT 1 cut(s) 204
MlyI GAGTC 4 cut(s) 598, 887, 1143, 1251
MmeI TCCRAC 2 cut(s) 254, 1050
MroNI GCCGGC 3 cut(s) 336, 610, 1038
MroXI GAANNNNTTC 1 cut(s) 1021
MseI TTAA 5 cut(s) 357, 504, 675, 783, 792
MslI CAYNNNNRTG 1 cut(s) 439
MspA1I CMGCKG 2 cut(s) 225, 1548
MunI CAATTG 1 cut(s) 840
MvaI CCWGG 5 cut(s) 351, 578, 702, 1295, 1528
NaeI GCCGGC 3 cut(s) 338, 612, 1040
NciI CCSGG 8 cut(s) 74, 366, 646, 1072, 1073, 1086, 1087, 1395
NdeII GATC 8 cut(s) 175, 183, 536, 581, 831, 965, 1139, 1421
NgoMIV GCCGGC 3 cut(s) 336, 610, 1038
NlaIII CATG 3 cut(s) 444, 750, 1452
NlaIV GGNNCC 8 cut(s) 615, 643, 696, 711, 1037, 1077, 1127, 1398
NmeAIII GCCGAG 6 cut(s) 1035, 1133, 1148, 1304, 1381, 1533
NmuCI GTSAC 1 cut(s) 1300
NspI RCATGY 1 cut(s) 1452
PcsI WCGNNNNNNNCGW 8 cut(s) 203, 207, 935, 993, 1005, 1056, 1414, 1426
PdiI GCCGGC 3 cut(s) 338, 612, 1040
PdmI GAANNNNTTC 1 cut(s) 1021
PfeI GAWTC 2 cut(s) 728, 1225
Pfl23II CGTACG 1 cut(s) 207
PflFI GACNNNGTC 1 cut(s) 899
PfoI TCCNGGA 1 cut(s) 72
PinAI ACCGGT 2 cut(s) 294, 315
PkrI GCNGC 6 cut(s) 27, 84, 227, 972, 991, 1479
Ple19I CGATCG 3 cut(s) 178, 186, 1424
PleI GAGTC 4 cut(s) 598, 886, 1143, 1250
PpsI GAGTC 4 cut(s) 598, 886, 1143, 1250
PpuMI RGGWCCY 1 cut(s) 1397
PshBI ATTAAT 1 cut(s) 504
Psp1406I AACGTT 1 cut(s) 36
Psp5II RGGWCCY 1 cut(s) 1397
Psp6I CCWGG 5 cut(s) 349, 576, 700, 1293, 1526
PspEI GGTNACC 1 cut(s) 1540
PspGI CCWGG 5 cut(s) 349, 576, 700, 1293, 1526
PspLI CGTACG 1 cut(s) 207
PspN4I GGNNCC 8 cut(s) 615, 643, 696, 711, 1037, 1077, 1127, 1398
PspPI GGNCC 8 cut(s) 107, 276, 297, 948, 1036, 1044, 1397, 1479
PspPPI RGGWCCY 1 cut(s) 1397
PsyI GACNNNGTC 1 cut(s) 899
PvuI CGATCG 3 cut(s) 178, 186, 1424
RsaI GTAC 3 cut(s) 209, 916, 1093
RsaNI GTAC 3 cut(s) 208, 915, 1092
RseI CAYNNNNRTG 1 cut(s) 439
Rsr2I CGGWCCG 1 cut(s) 297
RsrII CGGWCCG 1 cut(s) 297
SalI GTCGAC 2 cut(s) 235, 290
SaqAI TTAA 5 cut(s) 357, 504, 675, 783, 792
SatI GCNGC 6 cut(s) 26, 83, 226, 971, 990, 1478
Sau3AI GATC 8 cut(s) 175, 183, 536, 581, 831, 965, 1139, 1421
Sau96I GGNCC 8 cut(s) 107, 276, 297, 948, 1036, 1044, 1397, 1479
SchI GAGTC 4 cut(s) 598, 887, 1143, 1251
SduI GDGCHC 8 cut(s) 67, 315, 410, 529, 697, 1056, 1080, 1287
SfaNI GCATC 3 cut(s) 125, 559, 1187
SgrAI CRCCGGYG 1 cut(s) 1104
SinI GGWCC 5 cut(s) 107, 276, 297, 948, 1397
SmaI CCCGGG 2 cut(s) 1073, 1087
SmiMI CAYNNNNRTG 1 cut(s) 439
Sse9I AATT 7 cut(s) 358, 398, 681, 784, 840, 861, 1217
SspMI CTAG 3 cut(s) 551, 819, 867
StyI CCWWGG 3 cut(s) 279, 489, 1121
TaaI ACNGT 3 cut(s) 215, 241, 547
TaiI ACGT 5 cut(s) 39, 903, 1002, 1032, 1194
TaqII GACCGA 1 cut(s) 95
TasI AATT 7 cut(s) 358, 398, 681, 784, 840, 861, 1217
TatI WGTACW 1 cut(s) 914
TauI GCSGC 3 cut(s) 85, 973, 1480
TfiI GAWTC 2 cut(s) 728, 1225
Tru1I TTAA 5 cut(s) 357, 504, 675, 783, 792
Tru9I TTAA 5 cut(s) 357, 504, 675, 783, 792
TseFI GTSAC 1 cut(s) 1300
TseI GCWGC 3 cut(s) 25, 225, 989
Tsp45I GTSAC 1 cut(s) 1300
TspDTI ATGAA 2 cut(s) 490, 1199
TspGWI ACGGA 5 cut(s) 109, 242, 702, 778, 1175
TspMI CCCGGG 2 cut(s) 1071, 1085
Tth111I GACNNNGTC 1 cut(s) 899
VneI GTGCAC 1 cut(s) 311
VpaK11BI GGWCC 5 cut(s) 107, 276, 297, 948, 1397
VspI ATTAAT 1 cut(s) 504
XapI RAATTY 1 cut(s) 861
XceI RCATGY 1 cut(s) 1452
XmaI CCCGGG 2 cut(s) 1071, 1085
XmiI GTMKAC 2 cut(s) 236, 291
XmnI GAANNNNTTC 1 cut(s) 1021
XspI CTAG 3 cut(s) 551, 819, 867
ZraI GACGTC 2 cut(s) 1000, 1030
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.