pycom2575g00050

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00002575
OG0002208
Physical Location & Seq
Reverse (-)
14880 .. 17796
2917 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom2575g00050.4

Sequence Viewer

Length: 1894 bp
GCTCCGTGCTTCGGCATCGAACTGTCGGCTCGTACCCCGCTTCATCGACGCGTCGAGATTCGTCTCGTGCGCAGGATGGGTTTCTGTTTTGGCTACCTGCTGCGAAGGAACGTTGCCTCTCGTCGTCCCTTTCCTCCTCCTGCCGGCCTCGCCGGCGGGAGGACGACATCGTGGCGGTGCTCGTGTCCCGGACGAGCCGCACTCGTTGCGGTGCAGTTTCGGTCCTCGGGTTTCCGTTCGACATCTCGGATGCGGAACGCTGAAGGGGCGGTGGGTCTTCACGACCTCCGATCGTCCGATCGAAGCCTTGTCGCTCGACGCGTACGACCGTCGCGCCCGCTGCGAGCCGTCGACCGTCAAGTGTAAGTATGAACAAATTCAGACTGTGAAACTTTGGACCTTGGGTTGGGTCGACCGGTCCGCCTCTGGTGTGCACCGGTCGGCTCGTCCCTTCTGCCGGCGATACGCTCCTGGCCTTAATTGGCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGAATACATTAGCATGGGATAACATCATAGGACGAACAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGGCGGATGTTACTTTTAGGACTCCGCCGGCACCTTATGAGAAATCAAAGTTTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGGTCATCCTCCGCGGCCAGCTTCTTAGAGGGACTATGGCCATCATTGCAATTGTTGGTCTTCAACGAGGAATTCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTCCTACCGATTGAATGGTCCGGTGAAGTGTTCGGATCGCGGCGACGCGGGTGGTTCGCTGCCCGCGACGTCGCGAGAAGTGAACCTGCGGAAGGATCATTGTCGAACCTGCGCAGCAGAACGACCCGAGAACCCGTTCCCACGTCGGGGGCCGGCGGGCCTTCGGGCTCGGCGTCCCCTTATCCCGGGAGCCCGCTCCCGGGCGTACCAACGAACACCGGCGCGTCTTTGCGCCAAGGAACCCGAACGAAAGAGCGCGCTCCCGCCGCCCCGGAAACGGTGCGCGCGCGGGCGGCGTCGTCGTCTTCTGTAAAGTCAAAACGACTCTCGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTGAATTGCAGAATCCCGTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAAGCCGTTAGGCCGAGGGCACGCCTGCCTGGGCGTCACACGCCGTTGCCCCCCCGCGCCTCCCCCGGGAGCGTCGGGGGGCGGACGTTGGCCTCCCGTGCGTCCCCCCGCGCGGTTGGCACAAATGCCGGGTCCTCGGCGACGAACGCCACGACAATCGGTGGTTGTCGAACCTCGGTTGCCCGTTGTGCGCCTTCGTCGCGCCCGAGCGGCCCGCGACGCTCCCCGCTCCGCTTCGGCCGAGCTTTCAACGCGACCCCAGGTCAGGCGGGGTTACCCGCTGAATTTAAGCATATCAATAAGCGGAGGAAAAGAAACTTACGAGGATTCCCCTAGTAACGGCGAGCGAACCGGGAACAGCCCAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

631

Amino Acids

67.11

Weight (kDa)

11.62

Isoelectric Point (pI)

68.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1156
Acc16I TGCGCA 2 cut(s) 71, 1197
Acc36I ACCTGC 3 cut(s) 105, 1178, 1201
AccB1I GGYRCC 2 cut(s) 728, 809
AccBSI CCGCTC 3 cut(s) 1280, 1734, 1753
AccI GTMKAC 2 cut(s) 351, 412
AclI AACGTT 1 cut(s) 111
AclWI GGATC 3 cut(s) 704, 1127, 1187
AcoI YGGCCR 5 cut(s) 483, 864, 959, 982, 1762
AcsI RAATTY 3 cut(s) 376, 1015, 1808
AcuI CTGAAG 1 cut(s) 282
AcyI GRCGYC 4 cut(s) 1153, 1257, 1380, 1558
AfaI GTAC 4 cut(s) 34, 324, 1070, 1291
AflIII ACRYGT 2 cut(s) 49, 319
AgeI ACCGGT 2 cut(s) 415, 436
AgsI TTSAA 5 cut(s) 515, 1008, 1098, 1509, 1774
AhdI GACNNNNNGTC 1 cut(s) 1785
AjiI CACGTC 1 cut(s) 1228
AjnI CCWGG 5 cut(s) 470, 691, 815, 1552, 1783
AjuI GAANNNNNNNTTGG 2 cut(s) 597, 629
AluBI AGCT 5 cut(s) 937, 965, 1041, 1769, 1890
AluI AGCT 5 cut(s) 937, 965, 1041, 1769, 1890
Alw21I GWGCWC 3 cut(s) 182, 436, 531
Alw26I GTCTC 3 cut(s) 68, 674, 910
Alw44I GTGCAC 1 cut(s) 432
AlwI GGATC 3 cut(s) 704, 1127, 1187
Ama87I CYCGRG 6 cut(s) 226, 1210, 1269, 1283, 1589, 1729
ApaLI GTGCAC 1 cut(s) 432
ApeKI GCWGC 4 cut(s) 100, 340, 1143, 1198
ApoI RAATTY 3 cut(s) 376, 1015, 1808
ArsI GACNNNNNNTTYG 2 cut(s) 1640, 1672
AseI ATTAAT 1 cut(s) 619
AsiGI ACCGGT 2 cut(s) 415, 436
Asp700I GAANNNNTTC 2 cut(s) 376, 1219
AspS9I GGNCC 8 cut(s) 222, 397, 418, 1102, 1234, 1242, 1656, 1736
AsuHPI GGTGA 1 cut(s) 1119
AvaI CYCGRG 6 cut(s) 226, 1210, 1269, 1283, 1589, 1729
AvaII GGWCC 5 cut(s) 222, 397, 418, 1102, 1656
BaeGI GKGCMC 3 cut(s) 436, 812, 1546
BalI TGGCCA 1 cut(s) 984
BanI GGYRCC 2 cut(s) 728, 809
BanII GRGCYC 3 cut(s) 644, 1254, 1278
BauI CACGAG 2 cut(s) 65, 181
BbsI GAAGAC 4 cut(s) 269, 653, 996, 1381
Bbv12I GWGCWC 3 cut(s) 182, 436, 531
BbvCI CCTCAGC 1 cut(s) 921
BbvI GCAGC 4 cut(s) 87, 327, 1130, 1210
BccI CCATC 3 cut(s) 70, 993, 1504
BceAI ACGGC 5 cut(s) 332, 851, 1514, 1552, 1880
BciT130I CCWGG 5 cut(s) 472, 693, 817, 1554, 1785
BcoDI GTCTC 3 cut(s) 68, 674, 910
BfaI CTAG 4 cut(s) 666, 934, 1021, 1858
BfoI RGCGCY 1 cut(s) 505
BfuAI ACCTGC 3 cut(s) 105, 1178, 1201
BglI GCCNNNNNGGC 2 cut(s) 153, 1734
Bme18I GGWCC 5 cut(s) 222, 397, 418, 1102, 1656
BmeRI GACNNNNNGTC 1 cut(s) 1785
BmeT110I CYCGRG 6 cut(s) 226, 1210, 1269, 1283, 1589, 1729
BmgBI CACGTC 1 cut(s) 1228
BmgT120I GGNCC 8 cut(s) 222, 397, 418, 1102, 1234, 1242, 1656, 1736
BmiI GGNNCC 8 cut(s) 730, 758, 811, 826, 1235, 1275, 1325, 1657
BmsI GCATC 4 cut(s) 24, 240, 674, 1446
BpiI GAAGAC 4 cut(s) 269, 653, 996, 1381
BplI GAGNNNNNCTC 2 cut(s) 186, 218
Bpu10I CCTNAGC 1 cut(s) 921
Bsa29I ATCGAT 1 cut(s) 1440
BsaHI GRCGYC 4 cut(s) 1153, 1257, 1380, 1558
BsaI GGTCTC 1 cut(s) 910
BsaWI WCCGGW 3 cut(s) 415, 436, 1104
Bse118I RCCGGY 8 cut(s) 143, 152, 415, 436, 457, 725, 1236, 1302
Bse3DI GCAATG 1 cut(s) 988
BseBI CCWGG 5 cut(s) 472, 693, 817, 1554, 1785
BseCI ATCGAT 1 cut(s) 1440
BseGI GGATG 5 cut(s) 81, 255, 614, 710, 949
BseMI GCAATG 1 cut(s) 988
BseMII CTCAG 1 cut(s) 935
BsePI GCGCGC 3 cut(s) 1340, 1367, 1369
BseRI GAGGAG 1 cut(s) 126
BseSI GKGCMC 3 cut(s) 436, 812, 1546
BseX3I CGGCCG 1 cut(s) 1762
BseXI GCAGC 4 cut(s) 87, 327, 1130, 1210
BseYI CCCAGC 1 cut(s) 1886
BsgI GTGCAG 1 cut(s) 233
Bsh1285I CGRYCG 7 cut(s) 293, 301, 329, 355, 416, 441, 1765
BshNI GGYRCC 2 cut(s) 728, 809
BshTI ACCGGT 2 cut(s) 415, 436
BshVI ATCGAT 1 cut(s) 1440
BsiEI CGRYCG 7 cut(s) 293, 301, 329, 355, 416, 441, 1765
BsiHKAI GWGCWC 3 cut(s) 182, 436, 531
BsiHKCI CYCGRG 6 cut(s) 226, 1210, 1269, 1283, 1589, 1729
BsiWI CGTACG 1 cut(s) 322
BslFI GGGAC 7 cut(s) 111, 171, 433, 989, 1041, 1244, 1612
BsmAI GTCTC 3 cut(s) 68, 674, 910
BsmBI CGTCTC 1 cut(s) 68
BsmFI GGGAC 7 cut(s) 111, 171, 433, 989, 1041, 1244, 1612
Bso31I GGTCTC 1 cut(s) 910
BsoBI CYCGRG 6 cut(s) 226, 1210, 1269, 1283, 1589, 1729
Bsp1286I GDGCHC 8 cut(s) 182, 436, 531, 644, 812, 1254, 1278, 1546
Bsp1407I TGTACA 1 cut(s) 1068
Bsp143I GATC 6 cut(s) 290, 298, 651, 696, 1119, 1179
Bsp68I TCGCGA 1 cut(s) 1158
BspCNI CTCAG 1 cut(s) 934
BspDI ATCGAT 1 cut(s) 1440
BspLI GGNNCC 8 cut(s) 730, 758, 811, 826, 1235, 1275, 1325, 1657
BspMI ACCTGC 3 cut(s) 105, 1178, 1201
BspPI GGATC 3 cut(s) 704, 1127, 1187
BspT107I GGYRCC 2 cut(s) 728, 809
BspTNI GGTCTC 1 cut(s) 910
BsrBI CCGCTC 3 cut(s) 1280, 1734, 1753
BsrDI GCAATG 1 cut(s) 988
BsrFI RCCGGY 8 cut(s) 143, 152, 415, 436, 457, 725, 1236, 1302
BsrGI TGTACA 1 cut(s) 1068
BssAI RCCGGY 8 cut(s) 143, 152, 415, 436, 457, 725, 1236, 1302
BssHII GCGCGC 3 cut(s) 1340, 1367, 1369
BssMI GATC 6 cut(s) 290, 298, 651, 696, 1119, 1179
BssNI GRCGYC 4 cut(s) 1153, 1257, 1380, 1558
BssSI CACGAG 2 cut(s) 65, 181
BssT1I CCWWGG 3 cut(s) 400, 604, 1319
Bst2BI CACGAG 2 cut(s) 65, 181
Bst2UI CCWGG 5 cut(s) 472, 693, 817, 1554, 1785
Bst4CI ACNGT 6 cut(s) 24, 330, 356, 386, 662, 1364
BstACI GRCGYC 4 cut(s) 1153, 1257, 1380, 1558
BstAPI GCANNNNNTGC 1 cut(s) 206
BstAUI TGTACA 1 cut(s) 1068
BstDEI CTNAG 3 cut(s) 872, 921, 969
BstDSI CCRYGG 1 cut(s) 956
BstEII GGTNACC 1 cut(s) 1797
BstENI CCTNNNNNAGG 1 cut(s) 1174
BstF5I GGATG 5 cut(s) 81, 255, 614, 710, 949
BstH2I RGCGCY 1 cut(s) 505
BstKTI GATC 6 cut(s) 293, 301, 654, 699, 1122, 1182
BstMAI GTCTC 3 cut(s) 68, 674, 910
BstMBI GATC 6 cut(s) 290, 298, 651, 696, 1119, 1179
BstMCI CGRYCG 7 cut(s) 293, 301, 329, 355, 416, 441, 1765
BstNI CCWGG 5 cut(s) 472, 693, 817, 1554, 1785
BstPI GGTNACC 1 cut(s) 1797
BstSLI GKGCMC 3 cut(s) 436, 812, 1546
BstV1I GCAGC 4 cut(s) 87, 327, 1130, 1210
BstV2I GAAGAC 4 cut(s) 269, 653, 996, 1381
BstZI CGGCCG 1 cut(s) 1762
Bsu15I ATCGAT 1 cut(s) 1440
BsuTUI ATCGAT 1 cut(s) 1440
BtgI CCRYGG 1 cut(s) 956
BtrI CACGTC 1 cut(s) 1228
BtsCI GGATG 5 cut(s) 81, 255, 614, 710, 949
BtuMI TCGCGA 1 cut(s) 1158
BveI ACCTGC 3 cut(s) 105, 1178, 1201
Cfr10I RCCGGY 8 cut(s) 143, 152, 415, 436, 457, 725, 1236, 1302
Cfr13I GGNCC 8 cut(s) 222, 397, 418, 1102, 1234, 1242, 1656, 1736
Cfr42I CCGCGG 1 cut(s) 959
Cfr9I CCCGGG 3 cut(s) 1269, 1283, 1589
ClaI ATCGAT 1 cut(s) 1440
CpoI CGGWCCG 1 cut(s) 418
Csp6I GTAC 4 cut(s) 33, 323, 1069, 1290
CspAI ACCGGT 2 cut(s) 415, 436
CspI CGGWCCG 1 cut(s) 418
CviAII CATG 2 cut(s) 562, 862
CviQI GTAC 4 cut(s) 33, 323, 1069, 1290
DdeI CTNAG 3 cut(s) 872, 921, 969
DpnI GATC 6 cut(s) 292, 300, 653, 698, 1121, 1181
DpnII GATC 6 cut(s) 290, 298, 651, 696, 1119, 1179
DriI GACNNNNNGTC 1 cut(s) 1785
EaeI YGGCCR 5 cut(s) 483, 864, 959, 982, 1762
EagI CGGCCG 1 cut(s) 1762
Eam1105I GACNNNNNGTC 1 cut(s) 1785
EciI GGCGGA 4 cut(s) 410, 712, 717, 1621
EclXI CGGCCG 1 cut(s) 1762
Eco130I CCWWGG 3 cut(s) 400, 604, 1319
Eco24I GRGCYC 3 cut(s) 644, 1254, 1278
Eco31I GGTCTC 1 cut(s) 910
Eco32I GATATC 1 cut(s) 1424
Eco47I GGWCC 5 cut(s) 222, 397, 418, 1102, 1656
Eco52I CGGCCG 1 cut(s) 1762
Eco57I CTGAAG 1 cut(s) 282
Eco88I CYCGRG 6 cut(s) 226, 1210, 1269, 1283, 1589, 1729
Eco91I GGTNACC 1 cut(s) 1797
EcoNI CCTNNNNNAGG 1 cut(s) 1174
EcoO109I RGGNCCY 1 cut(s) 1656
EcoO65I GGTNACC 1 cut(s) 1797
EcoRI GAATTC 1 cut(s) 1015
EcoRII CCWGG 5 cut(s) 470, 691, 815, 1552, 1783
EcoRV GATATC 1 cut(s) 1424
EcoT14I CCWWGG 3 cut(s) 400, 604, 1319
EcoT38I GRGCYC 3 cut(s) 644, 1254, 1278
ErhI CCWWGG 3 cut(s) 400, 604, 1319
Esp3I CGTCTC 1 cut(s) 68
FaeI CATG 2 cut(s) 565, 865
FaqI GGGAC 7 cut(s) 111, 171, 433, 989, 1041, 1244, 1612
FatI CATG 2 cut(s) 561, 861
FblI GTMKAC 2 cut(s) 351, 412
FokI GGATG 5 cut(s) 88, 262, 621, 717, 936
FriOI GRGCYC 3 cut(s) 644, 1254, 1278
FspBI CTAG 4 cut(s) 666, 934, 1021, 1858
FspI TGCGCA 2 cut(s) 71, 1197
GsaI CCCAGC 1 cut(s) 1890
HaeII RGCGCY 1 cut(s) 505
Hin1I GRCGYC 4 cut(s) 1153, 1257, 1380, 1558
Hin1II CATG 2 cut(s) 565, 865
HincII GTYRAC 4 cut(s) 352, 413, 908, 1049
HindII GTYRAC 4 cut(s) 352, 413, 908, 1049
HinfI GANTC 8 cut(s) 58, 719, 843, 1032, 1408, 1484, 1501, 1851
HpaI GTTAAC 1 cut(s) 908
HphI GGTGA 1 cut(s) 1119
Hpy166II GTNNAC 8 cut(s) 352, 413, 434, 908, 1049, 1071, 1167, 1493
Hpy188I TCNGA 7 cut(s) 249, 290, 298, 382, 550, 656, 1119
Hpy188III TCNNGA 5 cut(s) 55, 281, 624, 840, 1157
Hpy8I GTNNAC 8 cut(s) 352, 413, 434, 908, 1049, 1071, 1167, 1493
HpyAV CCTTC 7 cut(s) 99, 257, 461, 799, 1170, 1255, 1728
HpyCH4III ACNGT 6 cut(s) 24, 330, 356, 386, 662, 1364
HpyCH4IV ACGT 6 cut(s) 111, 1054, 1153, 1227, 1450, 1610
HpyCH4V TGCA 5 cut(s) 214, 434, 861, 993, 1481
HpyF3I CTNAG 3 cut(s) 872, 921, 969
HpySE526I ACGT 6 cut(s) 111, 1054, 1153, 1227, 1450, 1610
Hsp92I GRCGYC 4 cut(s) 1153, 1257, 1380, 1558
Hsp92II CATG 2 cut(s) 565, 865
KroI GCCGGC 5 cut(s) 143, 152, 457, 725, 1236
KroNI GCCGGC 5 cut(s) 145, 154, 459, 727, 1238
KspAI GTTAAC 1 cut(s) 908
KspI CCGCGG 1 cut(s) 959
Kzo9I GATC 6 cut(s) 290, 298, 651, 696, 1119, 1179
Lsp1109I GCAGC 4 cut(s) 87, 327, 1130, 1210
LweI GCATC 4 cut(s) 24, 240, 674, 1446
MaeI CTAG 4 cut(s) 666, 934, 1021, 1858
MaeII ACGT 6 cut(s) 111, 1054, 1153, 1227, 1450, 1610
MaeIII GTNAC 5 cut(s) 506, 707, 1559, 1797, 1860
MalI GATC 6 cut(s) 292, 300, 653, 698, 1121, 1181
MauBI CGCGCGCG 1 cut(s) 1369
MbiI CCGCTC 3 cut(s) 1280, 1734, 1753
MboI GATC 6 cut(s) 290, 298, 651, 696, 1119, 1179
MboII GAAGA 6 cut(s) 269, 527, 658, 996, 1381, 1457
MfeI CAATTG 1 cut(s) 994
MhlI GDGCHC 8 cut(s) 182, 436, 531, 644, 812, 1254, 1278, 1546
MlsI TGGCCA 1 cut(s) 984
MluCI AATT 9 cut(s) 376, 479, 519, 796, 899, 994, 1015, 1476, 1808
MluI ACGCGT 2 cut(s) 49, 319
MluNI TGGCCA 1 cut(s) 984
MlyI GAGTC 4 cut(s) 713, 1041, 1402, 1510
Mox20I TGGCCA 1 cut(s) 984
MreI CGCCGGCG 1 cut(s) 152
MroNI GCCGGC 5 cut(s) 143, 152, 457, 725, 1236
MroXI GAANNNNTTC 2 cut(s) 376, 1219
MscI TGGCCA 1 cut(s) 984
MseI TTAA 6 cut(s) 478, 619, 790, 898, 907, 1812
MslI CAYNNNNRTG 1 cut(s) 560
Msp20I TGGCCA 1 cut(s) 984
MspA1I CMGCKG 3 cut(s) 340, 958, 1805
MunI CAATTG 1 cut(s) 994
MvaI CCWGG 5 cut(s) 472, 693, 817, 1554, 1785
NaeI GCCGGC 5 cut(s) 145, 154, 459, 727, 1238
NdeII GATC 6 cut(s) 290, 298, 651, 696, 1119, 1179
NgoMIV GCCGGC 5 cut(s) 143, 152, 457, 725, 1236
NlaIII CATG 2 cut(s) 565, 865
NlaIV GGNNCC 8 cut(s) 730, 758, 811, 826, 1235, 1275, 1325, 1657
NmeAIII GCCGAG 6 cut(s) 1233, 1392, 1407, 1563, 1640, 1790
NmuCI GTSAC 1 cut(s) 1559
NruI TCGCGA 1 cut(s) 1158
NsbI TGCGCA 2 cut(s) 71, 1197
PauI GCGCGC 3 cut(s) 1340, 1367, 1369
PcsI WCGNNNNNNNCGW 7 cut(s) 318, 322, 1089, 1147, 1254, 1673, 1727
PdiI GCCGGC 5 cut(s) 145, 154, 459, 727, 1238
PdmI GAANNNNTTC 2 cut(s) 376, 1219
PfeI GAWTC 4 cut(s) 58, 843, 1484, 1851
Pfl23II CGTACG 1 cut(s) 322
PflFI GACNNNGTC 1 cut(s) 1053
PfoI TCCNGGA 1 cut(s) 187
PinAI ACCGGT 2 cut(s) 415, 436
Ple19I CGATCG 2 cut(s) 293, 301
PleI GAGTC 4 cut(s) 713, 1040, 1402, 1509
PpsI GAGTC 4 cut(s) 713, 1040, 1402, 1509
PpuMI RGGWCCY 1 cut(s) 1656
PshBI ATTAAT 1 cut(s) 619
Psp1406I AACGTT 1 cut(s) 111
Psp5II RGGWCCY 1 cut(s) 1656
Psp6I CCWGG 5 cut(s) 470, 691, 815, 1552, 1783
PspEI GGTNACC 1 cut(s) 1797
PspFI CCCAGC 1 cut(s) 1886
PspGI CCWGG 5 cut(s) 470, 691, 815, 1552, 1783
PspLI CGTACG 1 cut(s) 322
PspN4I GGNNCC 8 cut(s) 730, 758, 811, 826, 1235, 1275, 1325, 1657
PspPI GGNCC 8 cut(s) 222, 397, 418, 1102, 1234, 1242, 1656, 1736
PspPPI RGGWCCY 1 cut(s) 1656
PsyI GACNNNGTC 1 cut(s) 1053
PteI GCGCGC 3 cut(s) 1340, 1367, 1369
PvuI CGATCG 2 cut(s) 293, 301
RruI TCGCGA 1 cut(s) 1158
RsaI GTAC 4 cut(s) 34, 324, 1070, 1291
RsaNI GTAC 4 cut(s) 33, 323, 1069, 1290
RseI CAYNNNNRTG 1 cut(s) 560
Rsr2I CGGWCCG 1 cut(s) 418
RsrII CGGWCCG 1 cut(s) 418
SacII CCGCGG 1 cut(s) 959
SalI GTCGAC 2 cut(s) 350, 411
SaqAI TTAA 6 cut(s) 478, 619, 790, 898, 907, 1812
Sau3AI GATC 6 cut(s) 290, 298, 651, 696, 1119, 1179
Sau96I GGNCC 8 cut(s) 222, 397, 418, 1102, 1234, 1242, 1656, 1736
SchI GAGTC 4 cut(s) 713, 1041, 1402, 1510
SduI GDGCHC 8 cut(s) 182, 436, 531, 644, 812, 1254, 1278, 1546
SfaNI GCATC 4 cut(s) 24, 240, 674, 1446
Sfr303I CCGCGG 1 cut(s) 959
SgrAI CRCCGGYG 2 cut(s) 152, 1302
SgrBI CCGCGG 1 cut(s) 959
SinI GGWCC 5 cut(s) 222, 397, 418, 1102, 1656
SmaI CCCGGG 3 cut(s) 1271, 1285, 1591
SmiMI CAYNNNNRTG 1 cut(s) 560
Sse9I AATT 9 cut(s) 376, 479, 519, 796, 899, 994, 1015, 1476, 1808
SspMI CTAG 4 cut(s) 666, 934, 1021, 1858
StyI CCWWGG 3 cut(s) 400, 604, 1319
TaaI ACNGT 6 cut(s) 24, 330, 356, 386, 662, 1364
TaiI ACGT 6 cut(s) 114, 1057, 1156, 1230, 1453, 1613
TaqII GACCGA 1 cut(s) 210
TasI AATT 9 cut(s) 376, 479, 519, 796, 899, 994, 1015, 1476, 1808
TatI WGTACW 1 cut(s) 1068
TauI GCSGC 6 cut(s) 200, 961, 1127, 1353, 1380, 1737
TfiI GAWTC 4 cut(s) 58, 843, 1484, 1851
Tru1I TTAA 6 cut(s) 478, 619, 790, 898, 907, 1812
Tru9I TTAA 6 cut(s) 478, 619, 790, 898, 907, 1812
TseFI GTSAC 1 cut(s) 1559
TseI GCWGC 4 cut(s) 100, 340, 1143, 1198
Tsp45I GTSAC 1 cut(s) 1559
TspDTI ATGAA 4 cut(s) 32, 385, 605, 1458
TspGWI ACGGA 4 cut(s) 224, 817, 893, 1434
TspMI CCCGGG 3 cut(s) 1269, 1283, 1589
Tth111I GACNNNGTC 1 cut(s) 1053
VneI GTGCAC 1 cut(s) 432
VpaK11BI GGWCC 5 cut(s) 222, 397, 418, 1102, 1656
VspI ATTAAT 1 cut(s) 619
XagI CCTNNNNNAGG 1 cut(s) 1174
XapI RAATTY 3 cut(s) 376, 1015, 1808
XmaI CCCGGG 3 cut(s) 1269, 1283, 1589
XmiI GTMKAC 2 cut(s) 351, 412
XmnI GAANNNNTTC 2 cut(s) 376, 1219
XspI CTAG 4 cut(s) 666, 934, 1021, 1858
ZraI GACGTC 1 cut(s) 1154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.