pycom553g00020

Conserved gene of

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_553
OG0002208
Physical Location & Seq
Forward (+)
2970 .. 3422
453 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom553g00020.1

Sequence Viewer

Length: 453 bp
ATGAAGAACGTAGCGAAATGCGATACTTGGTGTGAATTGCAGAATCCCGTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAAGCCGTTAGGCCGAGGGCACGCCTGCCTGGGCGTCACACGCCGTTGCCCCCCCGCGCCTCCCTCGGGAGCGTCGGGGGGCGGACGTTGGCCTCCCGTGCGTCCCCCCGCGCGGTTGGCACAAATGCCGGGTCCTCGGCGACGAACGCCACGACAATCGGTGGTTGTCGAACCTCGGTTGCCCGTTGTGCGCCTTCGTCGCGCCCGAGCGGCCCGCGACGCTCCCCGCTCCGCTTCGGCCGAGCTTTCAACGCGACCCCAGGTCAGGCGGGGTTACCCGCTGAATTTAAGCATATCAATAAGCGGAGGAAAAGAAACTTACGAGGATTCCCCTAGTAACGGCGAGCGAACCGGGAACAGCCCAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

151

Amino Acids

16.45

Weight (kDa)

11.63

Isoelectric Point (pI)

79.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 293, 312
AccII CGCG 6 cut(s) 140, 194, 196, 285, 300, 337
AcoI YGGCCR 1 cut(s) 321
AcsI RAATTY 1 cut(s) 367
AcyI GRCGYC 1 cut(s) 117
AfiI CCNNNNNNNGG 4 cut(s) 149, 195, 348, 422
AgsI TTSAA 2 cut(s) 68, 333
AhdI GACNNNNNGTC 1 cut(s) 344
AjnI CCWGG 2 cut(s) 111, 342
AluBI AGCT 2 cut(s) 328, 449
AluI AGCT 2 cut(s) 328, 449
Ama87I CYCGRG 2 cut(s) 148, 288
AoxI GGCC 4 cut(s) 94, 173, 294, 321
ApoI RAATTY 1 cut(s) 367
ArsI GACNNNNNNTTYG 2 cut(s) 199, 231
AspLEI GCGC 5 cut(s) 81, 142, 196, 276, 287
AspS9I GGNCC 2 cut(s) 215, 295
AsuC2I CCSGG 2 cut(s) 213, 436
AvaI CYCGRG 2 cut(s) 148, 288
AvaII GGWCC 1 cut(s) 215
BaeGI GKGCMC 1 cut(s) 105
BccI CCATC 1 cut(s) 63
BceAI ACGGC 3 cut(s) 73, 111, 439
BciT130I CCWGG 2 cut(s) 113, 344
BcnI CCSGG 2 cut(s) 213, 436
BfaI CTAG 1 cut(s) 417
BglI GCCNNNNNGGC 1 cut(s) 293
BisI GCNGC 1 cut(s) 294
BlsI GCNGC 1 cut(s) 295
Bme1390I CCNGG 4 cut(s) 113, 213, 344, 436
Bme18I GGWCC 1 cut(s) 215
BmeRI GACNNNNNGTC 1 cut(s) 344
BmeT110I CYCGRG 2 cut(s) 148, 288
BmgT120I GGNCC 2 cut(s) 215, 295
BmiI GGNNCC 1 cut(s) 216
BmrFI CCNGG 4 cut(s) 113, 213, 344, 436
BpuMI CCSGG 2 cut(s) 213, 436
BsaHI GRCGYC 1 cut(s) 117
BsaJI CCNNGG 6 cut(s) 97, 112, 147, 218, 257, 342
Bsc4I CCNNNNNNNGG 4 cut(s) 149, 195, 348, 422
BseBI CCWGG 2 cut(s) 113, 344
BseDI CCNNGG 6 cut(s) 97, 112, 147, 218, 257, 342
BseLI CCNNNNNNNGG 4 cut(s) 149, 195, 348, 422
BseSI GKGCMC 1 cut(s) 105
BseX3I CGGCCG 1 cut(s) 321
BseYI CCCAGC 1 cut(s) 445
Bsh1236I CGCG 6 cut(s) 140, 194, 196, 285, 300, 337
Bsh1285I CGRYCG 1 cut(s) 324
BshFI GGCC 4 cut(s) 96, 175, 296, 323
BsiEI CGRYCG 1 cut(s) 324
BsiHKCI CYCGRG 2 cut(s) 148, 288
BsiSI CCGG 2 cut(s) 212, 435
BslFI GGGAC 1 cut(s) 171
BslI CCNNNNNNNGG 4 cut(s) 149, 195, 348, 422
BsmFI GGGAC 1 cut(s) 171
BsnI GGCC 4 cut(s) 96, 175, 296, 323
BsoBI CYCGRG 2 cut(s) 148, 288
Bsp1286I GDGCHC 1 cut(s) 105
BspANI GGCC 4 cut(s) 96, 175, 296, 323
BspFNI CGCG 6 cut(s) 140, 194, 196, 285, 300, 337
BspLI GGNNCC 1 cut(s) 216
BsrBI CCGCTC 2 cut(s) 293, 312
BssECI CCNNGG 6 cut(s) 97, 112, 147, 218, 257, 342
BssNI GRCGYC 1 cut(s) 117
Bst2UI CCWGG 2 cut(s) 113, 344
BstACI GRCGYC 1 cut(s) 117
BstC8I GCNNGC 4 cut(s) 105, 109, 298, 428
BstEII GGTNACC 1 cut(s) 356
BstFNI CGCG 6 cut(s) 140, 194, 196, 285, 300, 337
BstHHI GCGC 5 cut(s) 81, 142, 196, 276, 287
BstMCI CGRYCG 1 cut(s) 324
BstNI CCWGG 2 cut(s) 113, 344
BstPI GGTNACC 1 cut(s) 356
BstSCI CCNGG 4 cut(s) 111, 211, 342, 434
BstSLI GKGCMC 1 cut(s) 105
BstUI CGCG 6 cut(s) 140, 194, 196, 285, 300, 337
BstZI CGGCCG 1 cut(s) 321
BsuRI GGCC 4 cut(s) 96, 175, 296, 323
Cac8I GCNNGC 4 cut(s) 105, 109, 298, 428
CfoI GCGC 5 cut(s) 81, 142, 196, 276, 287
Cfr13I GGNCC 2 cut(s) 215, 295
CseI GACGC 4 cut(s) 106, 144, 173, 311
CviJI RGCY 8 cut(s) 88, 96, 175, 296, 323, 328, 444, 449
CviKI_1 RGCY 8 cut(s) 88, 96, 175, 296, 323, 328, 444, 449
DriI GACNNNNNGTC 1 cut(s) 344
EaeI YGGCCR 1 cut(s) 321
EagI CGGCCG 1 cut(s) 321
Eam1105I GACNNNNNGTC 1 cut(s) 344
EciI GGCGGA 1 cut(s) 180
EclXI CGGCCG 1 cut(s) 321
Eco47I GGWCC 1 cut(s) 215
Eco52I CGGCCG 1 cut(s) 321
Eco88I CYCGRG 2 cut(s) 148, 288
Eco91I GGTNACC 1 cut(s) 356
EcoO109I RGGNCCY 1 cut(s) 215
EcoO65I GGTNACC 1 cut(s) 356
EcoRII CCWGG 2 cut(s) 111, 342
FaiI YATR 1 cut(s) 377
FaqI GGGAC 1 cut(s) 171
FauI CCCGC 6 cut(s) 145, 199, 305, 317, 345, 369
Fnu4HI GCNGC 1 cut(s) 294
Fsp4HI GCNGC 1 cut(s) 294
FspBI CTAG 1 cut(s) 417
GlaI GCGC 5 cut(s) 80, 141, 195, 275, 286
GluI GCNGC 1 cut(s) 294
GsaI CCCAGC 1 cut(s) 449
HaeIII GGCC 4 cut(s) 96, 175, 296, 323
HapII CCGG 2 cut(s) 212, 435
HgaI GACGC 4 cut(s) 106, 144, 173, 311
HhaI GCGC 5 cut(s) 81, 142, 196, 276, 287
Hin1I GRCGYC 1 cut(s) 117
Hin6I GCGC 5 cut(s) 79, 140, 194, 274, 285
HinP1I GCGC 5 cut(s) 79, 140, 194, 274, 285
HinfI GANTC 3 cut(s) 43, 60, 410
HpaII CCGG 2 cut(s) 212, 435
Hpy166II GTNNAC 1 cut(s) 52
Hpy188III TCNNGA 1 cut(s) 150
Hpy8I GTNNAC 1 cut(s) 52
Hpy99I CGWCG 4 cut(s) 160, 228, 285, 305
HpyAV CCTTC 1 cut(s) 287
HpyCH4IV ACGT 2 cut(s) 9, 169
HpyCH4V TGCA 1 cut(s) 40
HpySE526I ACGT 2 cut(s) 9, 169
Hsp92I GRCGYC 1 cut(s) 117
HspAI GCGC 5 cut(s) 79, 140, 194, 274, 285
LmnI GCTCC 3 cut(s) 152, 310, 317
LpnPI CCDG 8 cut(s) 98, 121, 125, 225, 329, 334, 356, 448
MaeI CTAG 1 cut(s) 417
MaeII ACGT 2 cut(s) 9, 169
MaeIII GTNAC 3 cut(s) 118, 356, 419
MbiI CCGCTC 2 cut(s) 293, 312
MboII GAAGA 1 cut(s) 16
MhlI GDGCHC 1 cut(s) 105
MluCI AATT 2 cut(s) 35, 367
MlyI GAGTC 1 cut(s) 69
MnlI CCTC 8 cut(s) 92, 153, 157, 186, 228, 267, 383, 400
MseI TTAA 1 cut(s) 371
MspA1I CMGCKG 1 cut(s) 364
MspI CCGG 2 cut(s) 212, 435
MspR9I CCNGG 4 cut(s) 113, 213, 344, 436
MvaI CCWGG 2 cut(s) 113, 344
MvnI CGCG 6 cut(s) 140, 194, 196, 285, 300, 337
NciI CCSGG 2 cut(s) 213, 436
NlaIV GGNNCC 1 cut(s) 216
NmeAIII GCCGAG 3 cut(s) 122, 199, 349
NmuCI GTSAC 1 cut(s) 118
PcsI WCGNNNNNNNCGW 2 cut(s) 232, 286
PfeI GAWTC 2 cut(s) 43, 410
PkrI GCNGC 1 cut(s) 295
PleI GAGTC 1 cut(s) 68
PpsI GAGTC 1 cut(s) 68
PpuMI RGGWCCY 1 cut(s) 215
Psp5II RGGWCCY 1 cut(s) 215
Psp6I CCWGG 2 cut(s) 111, 342
PspEI GGTNACC 1 cut(s) 356
PspFI CCCAGC 1 cut(s) 445
PspGI CCWGG 2 cut(s) 111, 342
PspN4I GGNNCC 1 cut(s) 216
PspPI GGNCC 2 cut(s) 215, 295
PspPPI RGGWCCY 1 cut(s) 215
SaqAI TTAA 1 cut(s) 371
SatI GCNGC 1 cut(s) 294
Sau96I GGNCC 2 cut(s) 215, 295
SchI GAGTC 1 cut(s) 69
ScrFI CCNGG 4 cut(s) 113, 213, 344, 436
SduI GDGCHC 1 cut(s) 105
SetI ASST 6 cut(s) 12, 172, 259, 330, 348, 451
SinI GGWCC 1 cut(s) 215
Sse9I AATT 2 cut(s) 35, 367
SspMI CTAG 1 cut(s) 417
StyD4I CCNGG 4 cut(s) 111, 211, 342, 434
TaiI ACGT 2 cut(s) 12, 172
TaqI TCGA 2 cut(s) 58, 252
TasI AATT 2 cut(s) 35, 367
TauI GCSGC 1 cut(s) 296
TfiI GAWTC 2 cut(s) 43, 410
Tru1I TTAA 1 cut(s) 371
Tru9I TTAA 1 cut(s) 371
TseFI GTSAC 1 cut(s) 118
Tsp45I GTSAC 1 cut(s) 118
TspDTI ATGAA 1 cut(s) 17
VpaK11BI GGWCC 1 cut(s) 215
XapI RAATTY 1 cut(s) 367
XspI CTAG 1 cut(s) 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.