pycom2246g00020

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00002246
OG0002208
Physical Location & Seq
Forward (+)
1855 .. 3043
1189 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom2246g00020.4

Sequence Viewer

Length: 939 bp
ATGAGTGGTAATTGGGTTGTATTCGACGGCAGGCTCCGTGCTTCGGCATCGAACTGTCGGCTCGTACCCCGCTTCATCGACGCGTCGAGATTTGTCTCGTGCGCAGGATGGGTTTCTGTTTTGGCTACCTGCTGCGAAGGAACGTTGCCTCTCGTCGTCCCTTTCCTCCTCTTGCTGGCCTCGCCGGCGGGAGGACGACATCGTGGCGGTGCTCGTGTCCCGGACGAGCCGCACTCGTTGCGGTGCAGTCTCGGTCCTCGGGTTTCCGTTCGACATCTCGGATGCGGAACCGAAGCCTTGTCGCTCGACGCGTACGACCGTCGCGCCCGCTGCGAGCCGTCGACCGTCAAGGTCGTCCGTCTCGCGCGACGCCGACGTCGGGAGGAATGCTACCTGGTCGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACAAATTCAGACTGTGAAACTCTGCTACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAACAAACCCCGACTTCTGGAAGGGACGCATTTATTAGATAAAAGGTCGACGCGGGCTCTGCCCGTTGCTCTGATGATTCATGATAACTCGACGGATCGCACAGCCATCGTGCTGGCGACGCATCATTCAAATATCTTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCAAATAACAATACCGGGCTCTACGAGTCTGGTAATTGGAATGAGTACAATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCCTCGTAGTTGGACCTTGGGTTGGGTCGACCGGTCCGCCTCTGGTGTGCACCGGTCGGCTCGTCCCTTCTGCCGGCGATACGCTCCTGGCCTTAATTGGCCGGGTCGTGCCTCCGGCGCTGTTACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

313

Amino Acids

34.32

Weight (kDa)

9.99

Isoelectric Point (pI)

49.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 375
AatII GACGTC 1 cut(s) 379
Acc16I TGCGCA 1 cut(s) 103
Acc36I ACCTGC 1 cut(s) 137
AccB1I GGYRCC 1 cut(s) 799
AccI GTMKAC 3 cut(s) 341, 569, 836
AccII CGCG 6 cut(s) 83, 311, 324, 365, 367, 574
AccIII TCCGGA 1 cut(s) 660
AciI CCGC 9 cut(s) 70, 188, 207, 230, 241, 285, 328, 574, 845
AclI AACGTT 1 cut(s) 143
AclWI GGATC 4 cut(s) 395, 624, 772, 785
AcoI YGGCCR 1 cut(s) 907
AcsI RAATTY 1 cut(s) 462
AcyI GRCGYC 2 cut(s) 370, 376
AfaI GTAC 3 cut(s) 66, 314, 755
AfiI CCNNNNNNNGG 9 cut(s) 43, 175, 191, 379, 538, 774, 787, 830, 881
AflIII ACRYGT 2 cut(s) 81, 309
AgeI ACCGGT 2 cut(s) 839, 860
AgsI TTSAA 1 cut(s) 651
AjnI CCWGG 2 cut(s) 393, 894
AluBI AGCT 1 cut(s) 512
AluI AGCT 1 cut(s) 512
Alw21I GWGCWC 2 cut(s) 214, 860
Alw26I GTCTC 4 cut(s) 100, 254, 365, 430
Alw44I GTGCAC 1 cut(s) 856
AlwI GGATC 4 cut(s) 395, 624, 772, 785
Ama87I CYCGRG 1 cut(s) 258
Aor13HI TCCGGA 1 cut(s) 660
AoxI GGCC 3 cut(s) 177, 897, 907
ApaLI GTGCAC 1 cut(s) 856
ApeKI GCWGC 4 cut(s) 132, 330, 703, 806
ApoI RAATTY 1 cut(s) 462
AsiGI ACCGGT 2 cut(s) 839, 860
Asp700I GAANNNNTTC 1 cut(s) 462
AspLEI GCGC 4 cut(s) 104, 326, 367, 928
AspS9I GGNCC 3 cut(s) 254, 821, 842
AsuC2I CCSGG 3 cut(s) 221, 724, 911
AvaI CYCGRG 1 cut(s) 258
AvaII GGWCC 3 cut(s) 254, 821, 842
BaeGI GKGCMC 1 cut(s) 860
BamHI GGATCC 1 cut(s) 777
BanI GGYRCC 1 cut(s) 799
BanII GRGCYC 2 cut(s) 580, 729
BauI CACGAG 2 cut(s) 97, 213
Bbv12I GWGCWC 2 cut(s) 214, 860
BbvI GCAGC 4 cut(s) 119, 317, 715, 818
BccI CCATC 2 cut(s) 102, 635
BceAI ACGGC 3 cut(s) 43, 322, 697
BcgI CGANNNNNNTGC 2 cut(s) 610, 644
BciT130I CCWGG 2 cut(s) 395, 896
BcnI CCSGG 3 cut(s) 221, 724, 911
BcoDI GTCTC 4 cut(s) 100, 254, 365, 430
BfaI CTAG 1 cut(s) 507
BfoI RGCGCY 1 cut(s) 929
BfuAI ACCTGC 1 cut(s) 137
BglI GCCNNNNNGGC 1 cut(s) 185
BisI GCNGC 5 cut(s) 133, 230, 331, 704, 807
BlsI GCNGC 5 cut(s) 134, 231, 332, 705, 808
Bme1390I CCNGG 5 cut(s) 221, 395, 724, 896, 911
Bme18I GGWCC 3 cut(s) 254, 821, 842
BmeT110I CYCGRG 1 cut(s) 258
BmgT120I GGNCC 3 cut(s) 254, 821, 842
BmiI GGNNCC 5 cut(s) 35, 289, 671, 779, 801
BmrFI CCNGG 5 cut(s) 221, 395, 724, 896, 911
BmsI GCATC 3 cut(s) 56, 272, 652
BplI GAGNNNNNCTC 2 cut(s) 218, 250
BpuMI CCSGG 3 cut(s) 221, 724, 911
BsaAI YACGTR 1 cut(s) 519
BsaHI GRCGYC 2 cut(s) 370, 376
BsaJI CCNNGG 3 cut(s) 257, 694, 824
BsaWI WCCGGW 3 cut(s) 660, 839, 860
BsaXI ACNNNNNCTCC 2 cut(s) 778, 808
Bsc4I CCNNNNNNNGG 9 cut(s) 43, 175, 191, 379, 538, 774, 787, 830, 881
Bse118I RCCGGY 4 cut(s) 184, 839, 860, 881
Bse1I ACTGG 1 cut(s) 408
BseAI TCCGGA 1 cut(s) 660
BseBI CCWGG 2 cut(s) 395, 896
BseDI CCNNGG 3 cut(s) 257, 694, 824
BseGI GGATG 3 cut(s) 113, 287, 690
BseLI CCNNNNNNNGG 9 cut(s) 43, 175, 191, 379, 538, 774, 787, 830, 881
BseMII CTCAG 1 cut(s) 665
BseNI ACTGG 1 cut(s) 408
BseRI GAGGAG 1 cut(s) 158
BseSI GKGCMC 1 cut(s) 860
BseXI GCAGC 4 cut(s) 119, 317, 715, 818
BsgI GTGCAG 1 cut(s) 265
Bsh1236I CGCG 6 cut(s) 83, 311, 324, 365, 367, 574
Bsh1285I CGRYCG 4 cut(s) 319, 345, 840, 865
BshFI GGCC 3 cut(s) 179, 899, 909
BshNI GGYRCC 1 cut(s) 799
BshTI ACCGGT 2 cut(s) 839, 860
BsiEI CGRYCG 4 cut(s) 319, 345, 840, 865
BsiHKAI GWGCWC 2 cut(s) 214, 860
BsiHKCI CYCGRG 1 cut(s) 258
BsiSI CCGG 9 cut(s) 185, 221, 661, 723, 840, 861, 882, 910, 923
BsiWI CGTACG 1 cut(s) 312
BslFI GGGAC 4 cut(s) 143, 203, 559, 857
BslI CCNNNNNNNGG 9 cut(s) 43, 175, 191, 379, 538, 774, 787, 830, 881
BsmAI GTCTC 4 cut(s) 100, 254, 365, 430
BsmBI CGTCTC 1 cut(s) 365
BsmFI GGGAC 4 cut(s) 143, 203, 559, 857
BsmI GAATGC 1 cut(s) 392
BsnI GGCC 3 cut(s) 179, 899, 909
BsoBI CYCGRG 1 cut(s) 258
Bsp1286I GDGCHC 4 cut(s) 214, 580, 729, 860
Bsp13I TCCGGA 1 cut(s) 660
Bsp143I GATC 3 cut(s) 400, 616, 777
BspACI CCGC 9 cut(s) 70, 188, 207, 230, 241, 285, 328, 574, 845
BspANI GGCC 3 cut(s) 179, 899, 909
BspCNI CTCAG 1 cut(s) 666
BspEI TCCGGA 1 cut(s) 660
BspFNI CGCG 6 cut(s) 83, 311, 324, 365, 367, 574
BspHI TCATGA 1 cut(s) 601
BspLI GGNNCC 5 cut(s) 35, 289, 671, 779, 801
BspMI ACCTGC 1 cut(s) 137
BspPI GGATC 4 cut(s) 395, 624, 772, 785
BspT107I GGYRCC 1 cut(s) 799
BsrFI RCCGGY 4 cut(s) 184, 839, 860, 881
BsrI ACTGG 1 cut(s) 408
BssAI RCCGGY 4 cut(s) 184, 839, 860, 881
BssECI CCNNGG 3 cut(s) 257, 694, 824
BssMI GATC 3 cut(s) 400, 616, 777
BssNI GRCGYC 2 cut(s) 370, 376
BssSI CACGAG 2 cut(s) 97, 213
BssT1I CCWWGG 2 cut(s) 694, 824
Bst2BI CACGAG 2 cut(s) 97, 213
Bst2UI CCWGG 2 cut(s) 395, 896
Bst4CI ACNGT 5 cut(s) 56, 320, 346, 472, 497
BstACI GRCGYC 2 cut(s) 370, 376
BstAPI GCANNNNNTGC 1 cut(s) 238
BstBAI YACGTR 1 cut(s) 519
BstDEI CTNAG 1 cut(s) 674
BstENI CCTNNNNNAGG 1 cut(s) 772
BstF5I GGATG 3 cut(s) 113, 287, 690
BstFNI CGCG 6 cut(s) 83, 311, 324, 365, 367, 574
BstH2I RGCGCY 1 cut(s) 929
BstHHI GCGC 4 cut(s) 104, 326, 367, 928
BstKTI GATC 3 cut(s) 403, 619, 780
BstMAI GTCTC 4 cut(s) 100, 254, 365, 430
BstMBI GATC 3 cut(s) 400, 616, 777
BstMCI CGRYCG 4 cut(s) 319, 345, 840, 865
BstMWI GCNNNNNNNGC 7 cut(s) 181, 185, 238, 330, 580, 640, 925
BstNI CCWGG 2 cut(s) 395, 896
BstNSI RCATGY 1 cut(s) 448
BstSCI CCNGG 5 cut(s) 219, 393, 722, 894, 909
BstSLI GKGCMC 1 cut(s) 860
BstUI CGCG 6 cut(s) 83, 311, 324, 365, 367, 574
BstV1I GCAGC 4 cut(s) 119, 317, 715, 818
BstX2I RGATCY 1 cut(s) 777
BstXI CCANNNNNNTGG 1 cut(s) 634
BstYI RGATCY 1 cut(s) 777
BsuRI GGCC 3 cut(s) 179, 899, 909
BtsCI GGATG 3 cut(s) 113, 287, 690
BveI ACCTGC 1 cut(s) 137
CciI TCATGA 1 cut(s) 601
CfoI GCGC 4 cut(s) 104, 326, 367, 928
Cfr10I RCCGGY 4 cut(s) 184, 839, 860, 881
Cfr13I GGNCC 3 cut(s) 254, 821, 842
CpoI CGGWCCG 1 cut(s) 842
CseI GACGC 7 cut(s) 72, 89, 317, 378, 556, 580, 649
CsiI ACCWGGT 1 cut(s) 393
Csp6I GTAC 3 cut(s) 65, 313, 754
CspAI ACCGGT 2 cut(s) 839, 860
CspI CGGWCCG 1 cut(s) 842
CviAII CATG 3 cut(s) 441, 445, 602
CviQI GTAC 3 cut(s) 65, 313, 754
DdeI CTNAG 1 cut(s) 674
DpnI GATC 3 cut(s) 402, 618, 779
DpnII GATC 3 cut(s) 400, 616, 777
DrdI GACNNNNNNGTC 1 cut(s) 375
DseDI GACNNNNNNGTC 1 cut(s) 375
EaeI YGGCCR 1 cut(s) 907
EciI GGCGGA 1 cut(s) 834
Eco130I CCWWGG 2 cut(s) 694, 824
Eco24I GRGCYC 2 cut(s) 580, 729
Eco47I GGWCC 3 cut(s) 254, 821, 842
Eco88I CYCGRG 1 cut(s) 258
EcoNI CCTNNNNNAGG 1 cut(s) 772
EcoRII CCWGG 2 cut(s) 393, 894
EcoT14I CCWWGG 2 cut(s) 694, 824
EcoT22I ATGCAT 1 cut(s) 446
EcoT38I GRGCYC 2 cut(s) 580, 729
ErhI CCWWGG 2 cut(s) 694, 824
Esp3I CGTCTC 1 cut(s) 365
FaeI CATG 3 cut(s) 444, 448, 605
FaiI YATR 6 cut(s) 417, 419, 442, 446, 456, 603
FaqI GGGAC 4 cut(s) 143, 203, 559, 857
FatI CATG 3 cut(s) 440, 444, 601
FauI CCCGC 4 cut(s) 77, 181, 335, 567
FauNDI CATATG 1 cut(s) 417
FblI GTMKAC 3 cut(s) 341, 569, 836
Fnu4HI GCNGC 5 cut(s) 133, 230, 331, 704, 807
FokI GGATG 3 cut(s) 120, 294, 677
FriOI GRGCYC 2 cut(s) 580, 729
Fsp4HI GCNGC 5 cut(s) 133, 230, 331, 704, 807
FspBI CTAG 1 cut(s) 507
FspI TGCGCA 1 cut(s) 103
GlaI GCGC 4 cut(s) 103, 325, 366, 927
GluI GCNGC 5 cut(s) 133, 230, 331, 704, 807
HaeII RGCGCY 1 cut(s) 929
HaeIII GGCC 3 cut(s) 179, 899, 909
HapII CCGG 9 cut(s) 185, 221, 661, 723, 840, 861, 882, 910, 923
HgaI GACGC 7 cut(s) 72, 89, 317, 378, 556, 580, 649
HhaI GCGC 4 cut(s) 104, 326, 367, 928
Hin1I GRCGYC 2 cut(s) 370, 376
Hin1II CATG 3 cut(s) 444, 448, 605
Hin6I GCGC 4 cut(s) 102, 324, 365, 926
HinP1I GCGC 4 cut(s) 102, 324, 365, 926
HincII GTYRAC 3 cut(s) 342, 570, 837
HindII GTYRAC 3 cut(s) 342, 570, 837
HinfI GANTC 2 cut(s) 598, 734
HpaII CCGG 9 cut(s) 185, 221, 661, 723, 840, 861, 882, 910, 923
Hpy166II GTNNAC 4 cut(s) 342, 570, 837, 858
Hpy188I TCNGA 4 cut(s) 281, 468, 490, 594
Hpy188III TCNNGA 6 cut(s) 87, 380, 507, 539, 602, 661
Hpy8I GTNNAC 4 cut(s) 342, 570, 837, 858
HpyAV CCTTC 4 cut(s) 131, 536, 693, 885
HpyCH4III ACNGT 5 cut(s) 56, 320, 346, 472, 497
HpyCH4IV ACGT 3 cut(s) 143, 376, 518
HpyCH4V TGCA 4 cut(s) 246, 444, 522, 858
HpyF10VI GCNNNNNNNGC 7 cut(s) 181, 185, 238, 330, 580, 640, 925
HpyF3I CTNAG 1 cut(s) 674
HpySE526I ACGT 3 cut(s) 143, 376, 518
Hsp92I GRCGYC 2 cut(s) 370, 376
Hsp92II CATG 3 cut(s) 444, 448, 605
HspAI GCGC 4 cut(s) 102, 324, 365, 926
Kpn2I TCCGGA 1 cut(s) 660
KroI GCCGGC 2 cut(s) 184, 881
KroNI GCCGGC 2 cut(s) 186, 883
Kzo9I GATC 3 cut(s) 400, 616, 777
LmnI GCTCC 3 cut(s) 39, 669, 897
Lsp1109I GCAGC 4 cut(s) 119, 317, 715, 818
LweI GCATC 3 cut(s) 56, 272, 652
MabI ACCWGGT 1 cut(s) 393
MaeI CTAG 1 cut(s) 507
MaeII ACGT 3 cut(s) 143, 376, 518
MaeIII GTNAC 1 cut(s) 930
MalI GATC 3 cut(s) 402, 618, 779
MboI GATC 3 cut(s) 400, 616, 777
MboII GAAGA 1 cut(s) 649
MflI RGATCY 1 cut(s) 777
MhlI GDGCHC 4 cut(s) 214, 580, 729, 860
MluCI AATT 5 cut(s) 10, 462, 502, 742, 903
MluI ACGCGT 2 cut(s) 81, 309
MlyI GAGTC 1 cut(s) 743
MmeI TCCRAC 1 cut(s) 799
Mph1103I ATGCAT 1 cut(s) 446
MreI CGCCGGCG 1 cut(s) 184
MroI TCCGGA 1 cut(s) 660
MroNI GCCGGC 2 cut(s) 184, 881
MroXI GAANNNNTTC 1 cut(s) 462
MseI TTAA 3 cut(s) 435, 770, 902
MslI CAYNNNNRTG 1 cut(s) 445
MspA1I CMGCKG 1 cut(s) 330
MspI CCGG 9 cut(s) 185, 221, 661, 723, 840, 861, 882, 910, 923
MspR9I CCNGG 5 cut(s) 221, 395, 724, 896, 911
Mva1269I GAATGC 1 cut(s) 392
MvaI CCWGG 2 cut(s) 395, 896
MvnI CGCG 6 cut(s) 83, 311, 324, 365, 367, 574
MwoI GCNNNNNNNGC 7 cut(s) 181, 185, 238, 330, 580, 640, 925
NaeI GCCGGC 2 cut(s) 186, 883
NciI CCSGG 3 cut(s) 221, 724, 911
NdeI CATATG 1 cut(s) 417
NdeII GATC 3 cut(s) 400, 616, 777
NgoMIV GCCGGC 2 cut(s) 184, 881
NlaIII CATG 3 cut(s) 444, 448, 605
NlaIV GGNNCC 5 cut(s) 35, 289, 671, 779, 801
NsbI TGCGCA 1 cut(s) 103
NsiI ATGCAT 1 cut(s) 446
NspI RCATGY 1 cut(s) 448
PagI TCATGA 1 cut(s) 601
PcsI WCGNNNNNNNCGW 2 cut(s) 308, 312
PctI GAATGC 1 cut(s) 392
PdiI GCCGGC 2 cut(s) 186, 883
PdmI GAANNNNTTC 1 cut(s) 462
PfeI GAWTC 1 cut(s) 598
Pfl23II CGTACG 1 cut(s) 312
PfoI TCCNGGA 1 cut(s) 219
PinAI ACCGGT 2 cut(s) 839, 860
PkrI GCNGC 5 cut(s) 134, 231, 332, 705, 808
PleI GAGTC 1 cut(s) 742
PpsI GAGTC 1 cut(s) 742
Ppu21I YACGTR 1 cut(s) 519
Psp1406I AACGTT 1 cut(s) 143
Psp6I CCWGG 2 cut(s) 393, 894
PspGI CCWGG 2 cut(s) 393, 894
PspLI CGTACG 1 cut(s) 312
PspN4I GGNNCC 5 cut(s) 35, 289, 671, 779, 801
PspPI GGNCC 3 cut(s) 254, 821, 842
PsuI RGATCY 1 cut(s) 777
RsaI GTAC 3 cut(s) 66, 314, 755
RsaNI GTAC 3 cut(s) 65, 313, 754
RseI CAYNNNNRTG 1 cut(s) 445
Rsr2I CGGWCCG 1 cut(s) 842
RsrII CGGWCCG 1 cut(s) 842
SalI GTCGAC 3 cut(s) 340, 568, 835
SaqAI TTAA 3 cut(s) 435, 770, 902
SatI GCNGC 5 cut(s) 133, 230, 331, 704, 807
Sau3AI GATC 3 cut(s) 400, 616, 777
Sau96I GGNCC 3 cut(s) 254, 821, 842
SchI GAGTC 1 cut(s) 743
ScrFI CCNGG 5 cut(s) 221, 395, 724, 896, 911
SduI GDGCHC 4 cut(s) 214, 580, 729, 860
SetI ASST 9 cut(s) 131, 146, 354, 379, 396, 514, 521, 569, 826
SexAI ACCWGGT 1 cut(s) 393
SfaNI GCATC 3 cut(s) 56, 272, 652
SgrAI CRCCGGYG 1 cut(s) 184
SinI GGWCC 3 cut(s) 254, 821, 842
SmiMI CAYNNNNRTG 1 cut(s) 445
Sse9I AATT 5 cut(s) 10, 462, 502, 742, 903
SsiI CCGC 9 cut(s) 70, 188, 207, 230, 241, 285, 328, 574, 845
SspMI CTAG 1 cut(s) 507
StyD4I CCNGG 5 cut(s) 219, 393, 722, 894, 909
StyI CCWWGG 2 cut(s) 694, 824
TaaI ACNGT 5 cut(s) 56, 320, 346, 472, 497
TaiI ACGT 3 cut(s) 146, 379, 521
TaqII GACCGA 1 cut(s) 242
TasI AATT 5 cut(s) 10, 462, 502, 742, 903
TatI WGTACW 1 cut(s) 753
TauI GCSGC 1 cut(s) 232
TfiI GAWTC 1 cut(s) 598
Tru1I TTAA 3 cut(s) 435, 770, 902
Tru9I TTAA 3 cut(s) 435, 770, 902
TseI GCWGC 4 cut(s) 132, 330, 703, 806
TspDTI ATGAA 3 cut(s) 64, 471, 590
TspGWI ACGGA 4 cut(s) 26, 256, 347, 629
VneI GTGCAC 1 cut(s) 856
VpaK11BI GGWCC 3 cut(s) 254, 821, 842
XagI CCTNNNNNAGG 1 cut(s) 772
XapI RAATTY 1 cut(s) 462
XbaI TCTAGA 1 cut(s) 506
XceI RCATGY 1 cut(s) 448
XmiI GTMKAC 3 cut(s) 341, 569, 836
XmnI GAANNNNTTC 1 cut(s) 462
XspI CTAG 1 cut(s) 507
ZraI GACGTC 1 cut(s) 377
Zsp2I ATGCAT 1 cut(s) 446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.