Rroxscaffold_31G00438260

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000031
OG0002208
Physical Location & Seq
Reverse (-)
170215 .. 170991
777 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_31G00438260.1

Sequence Viewer

Length: 492 bp
ATGTCTAAACGACTCTCGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTGAATTGCAGAATCCCGTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAAGCCATTAGGCCGAGGGCACGTCCGCCGGGCGTCACACGTCGTTGCCCCCCCCCAACCCCCTCGGGAGTTGGATGGGACGGATGATGGCCTCCCGTGTGCTCAGTCACGCGGTTGGCATAAATACCAAGTCCTCGGCGACCAACGCCACGACAATCGGGGCGCCATAGAGGGTGAGAGCCCCGTTGTGCCCGACCCCGTCGCACCACGAGGCGCTGTCGGCGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAAATTCCGTCCAAGGCTAAATACGGCGAGAGACCGATAGCAAACAAGTACCGCGAGGGAAAGATGAAAAGGACTTTGAAAAGAGAGTCAAAGAGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

163

Amino Acids

17.91

Weight (kDa)

9.97

Isoelectric Point (pI)

56.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 290
AccII CGCG 2 cut(s) 240, 444
AciI CCGC 4 cut(s) 154, 240, 387, 442
AcsI RAATTY 1 cut(s) 393
AcyI GRCGYC 2 cut(s) 161, 291
AfaI GTAC 1 cut(s) 440
AfiI CCNNNNNNNGG 1 cut(s) 383
AflIII ACRYGT 1 cut(s) 166
AgsI TTSAA 2 cut(s) 113, 469
AjiI CACGTC 2 cut(s) 151, 169
Alw21I GWGCWC 1 cut(s) 232
Alw26I GTCTC 1 cut(s) 415
Ama87I CYCGRG 1 cut(s) 192
AoxI GGCC 2 cut(s) 139, 217
ApeKI GCWGC 1 cut(s) 373
ApoI RAATTY 1 cut(s) 393
AspLEI GCGC 3 cut(s) 126, 293, 344
AsuC2I CCSGG 1 cut(s) 158
AsuHPI GGTGA 1 cut(s) 314
AvaI CYCGRG 1 cut(s) 192
BaeGI GKGCMC 2 cut(s) 150, 321
BanI GGYRCC 1 cut(s) 290
BanII GRGCYC 1 cut(s) 311
BauI CACGAG 1 cut(s) 336
Bbv12I GWGCWC 1 cut(s) 232
BbvI GCAGC 1 cut(s) 385
BccI CCATC 3 cut(s) 108, 197, 209
BceAI ACGGC 1 cut(s) 430
BcnI CCSGG 1 cut(s) 158
BcoDI GTCTC 1 cut(s) 415
BfoI RGCGCY 2 cut(s) 294, 345
BisI GCNGC 1 cut(s) 374
BlsI GCNGC 1 cut(s) 375
Bme1390I CCNGG 1 cut(s) 158
BmeT110I CYCGRG 1 cut(s) 192
BmgBI CACGTC 2 cut(s) 151, 169
BmiI GGNNCC 1 cut(s) 292
BmrFI CCNGG 1 cut(s) 158
BmsI GCATC 1 cut(s) 50
BpuMI CCSGG 1 cut(s) 158
Bsa29I ATCGAT 1 cut(s) 44
BsaHI GRCGYC 2 cut(s) 161, 291
BsaI GGTCTC 1 cut(s) 415
BsaJI CCNNGG 4 cut(s) 142, 191, 262, 402
Bsc4I CCNNNNNNNGG 1 cut(s) 383
BseCI ATCGAT 1 cut(s) 44
BseDI CCNNGG 4 cut(s) 142, 191, 262, 402
BseGI GGATG 2 cut(s) 208, 217
BseLI CCNNNNNNNGG 1 cut(s) 383
BseMII CTCAG 1 cut(s) 245
BseSI GKGCMC 2 cut(s) 150, 321
BseXI GCAGC 1 cut(s) 385
Bsh1236I CGCG 2 cut(s) 240, 444
BshFI GGCC 2 cut(s) 141, 219
BshNI GGYRCC 1 cut(s) 290
BshVI ATCGAT 1 cut(s) 44
BsiHKAI GWGCWC 1 cut(s) 232
BsiHKCI CYCGRG 1 cut(s) 192
BsiSI CCGG 1 cut(s) 157
BslFI GGGAC 1 cut(s) 220
BslI CCNNNNNNNGG 1 cut(s) 383
BsmAI GTCTC 1 cut(s) 415
BsmFI GGGAC 1 cut(s) 220
BsmI GAATGC 1 cut(s) 375
BsnI GGCC 2 cut(s) 141, 219
Bso31I GGTCTC 1 cut(s) 415
BsoBI CYCGRG 1 cut(s) 192
Bsp1286I GDGCHC 4 cut(s) 150, 232, 311, 321
BspACI CCGC 4 cut(s) 154, 240, 387, 442
BspANI GGCC 2 cut(s) 141, 219
BspCNI CTCAG 1 cut(s) 244
BspDI ATCGAT 1 cut(s) 44
BspFNI CGCG 2 cut(s) 240, 444
BspLI GGNNCC 1 cut(s) 292
BspT107I GGYRCC 1 cut(s) 290
BspTNI GGTCTC 1 cut(s) 415
BssECI CCNNGG 4 cut(s) 142, 191, 262, 402
BssNI GRCGYC 2 cut(s) 161, 291
BssSI CACGAG 1 cut(s) 336
BssT1I CCWWGG 1 cut(s) 402
Bst2BI CACGAG 1 cut(s) 336
BstACI GRCGYC 2 cut(s) 161, 291
BstDEI CTNAG 1 cut(s) 231
BstF5I GGATG 2 cut(s) 208, 217
BstFNI CGCG 2 cut(s) 240, 444
BstH2I RGCGCY 2 cut(s) 294, 345
BstHHI GCGC 3 cut(s) 126, 293, 344
BstMAI GTCTC 1 cut(s) 415
BstMWI GCNNNNNNNGC 4 cut(s) 123, 130, 273, 348
BstSCI CCNGG 1 cut(s) 156
BstSLI GKGCMC 2 cut(s) 150, 321
BstUI CGCG 2 cut(s) 240, 444
BstV1I GCAGC 1 cut(s) 385
Bsu15I ATCGAT 1 cut(s) 44
BsuRI GGCC 2 cut(s) 141, 219
BsuTUI ATCGAT 1 cut(s) 44
BtrI CACGTC 2 cut(s) 151, 169
BtsCI GGATG 2 cut(s) 208, 217
CfoI GCGC 3 cut(s) 126, 293, 344
ClaI ATCGAT 1 cut(s) 44
CseI GACGC 1 cut(s) 150
Csp6I GTAC 1 cut(s) 439
CviJI RGCY 7 cut(s) 35, 133, 141, 219, 309, 376, 407
CviKI_1 RGCY 7 cut(s) 35, 133, 141, 219, 309, 376, 407
CviQI GTAC 1 cut(s) 439
DdeI CTNAG 1 cut(s) 231
DinI GGCGCC 1 cut(s) 292
EciI GGCGGA 1 cut(s) 143
Eco130I CCWWGG 1 cut(s) 402
Eco24I GRGCYC 1 cut(s) 311
Eco31I GGTCTC 1 cut(s) 415
Eco32I GATATC 1 cut(s) 28
Eco88I CYCGRG 1 cut(s) 192
EcoRV GATATC 1 cut(s) 28
EcoT14I CCWWGG 1 cut(s) 402
EcoT38I GRGCYC 1 cut(s) 311
EgeI GGCGCC 1 cut(s) 292
EheI GGCGCC 1 cut(s) 292
ErhI CCWWGG 1 cut(s) 402
FaiI YATR 2 cut(s) 249, 296
FaqI GGGAC 1 cut(s) 220
Fnu4HI GCNGC 1 cut(s) 374
FokI GGATG 2 cut(s) 215, 224
FriOI GRGCYC 1 cut(s) 311
Fsp4HI GCNGC 1 cut(s) 374
GlaI GCGC 3 cut(s) 125, 292, 343
GluI GCNGC 1 cut(s) 374
HaeII RGCGCY 2 cut(s) 294, 345
HaeIII GGCC 2 cut(s) 141, 219
HapII CCGG 1 cut(s) 157
HgaI GACGC 1 cut(s) 150
HhaI GCGC 3 cut(s) 126, 293, 344
Hin1I GRCGYC 2 cut(s) 161, 291
Hin6I GCGC 3 cut(s) 124, 291, 342
HinP1I GCGC 3 cut(s) 124, 291, 342
HinfI GANTC 5 cut(s) 12, 88, 105, 353, 476
HpaII CCGG 1 cut(s) 157
HphI GGTGA 1 cut(s) 314
Hpy166II GTNNAC 1 cut(s) 97
Hpy188III TCNNGA 1 cut(s) 194
Hpy8I GTNNAC 1 cut(s) 97
Hpy99I CGWCG 2 cut(s) 173, 332
HpyCH4IV ACGT 3 cut(s) 54, 150, 168
HpyCH4V TGCA 2 cut(s) 85, 373
HpyF10VI GCNNNNNNNGC 4 cut(s) 123, 130, 273, 348
HpyF3I CTNAG 1 cut(s) 231
HpySE526I ACGT 3 cut(s) 54, 150, 168
Hsp92I GRCGYC 2 cut(s) 161, 291
HspAI GCGC 3 cut(s) 124, 291, 342
KasI GGCGCC 1 cut(s) 290
LpnPI CCDG 1 cut(s) 170
Lsp1109I GCAGC 1 cut(s) 385
LweI GCATC 1 cut(s) 50
MaeII ACGT 3 cut(s) 54, 150, 168
MaeIII GTNAC 2 cut(s) 162, 234
MboII GAAGA 1 cut(s) 61
MhlI GDGCHC 4 cut(s) 150, 232, 311, 321
MluCI AATT 2 cut(s) 80, 393
Mly113I GGCGCC 1 cut(s) 291
MlyI GAGTC 4 cut(s) 6, 114, 362, 485
MmeI TCCRAC 1 cut(s) 180
MnlI CCTC 7 cut(s) 137, 201, 230, 272, 292, 332, 439
MspI CCGG 1 cut(s) 157
MspR9I CCNGG 1 cut(s) 158
Mva1269I GAATGC 1 cut(s) 375
MvnI CGCG 2 cut(s) 240, 444
MwoI GCNNNNNNNGC 4 cut(s) 123, 130, 273, 348
NarI GGCGCC 1 cut(s) 291
NciI CCSGG 1 cut(s) 158
NlaIV GGNNCC 1 cut(s) 292
NmeAIII GCCGAG 3 cut(s) 11, 167, 243
NmuCI GTSAC 2 cut(s) 162, 234
PctI GAATGC 1 cut(s) 375
PfeI GAWTC 1 cut(s) 88
PflFI GACNNNGTC 1 cut(s) 326
PkrI GCNGC 1 cut(s) 375
PleI GAGTC 4 cut(s) 6, 113, 361, 484
PluTI GGCGCC 1 cut(s) 294
PpsI GAGTC 4 cut(s) 6, 113, 361, 484
PspN4I GGNNCC 1 cut(s) 292
PsyI GACNNNGTC 1 cut(s) 326
RsaI GTAC 1 cut(s) 440
RsaNI GTAC 1 cut(s) 439
SatI GCNGC 1 cut(s) 374
SchI GAGTC 4 cut(s) 6, 114, 362, 485
ScrFI CCNGG 1 cut(s) 158
SduI GDGCHC 4 cut(s) 150, 232, 311, 321
SetI ASST 3 cut(s) 57, 153, 171
SfaNI GCATC 1 cut(s) 50
SfoI GGCGCC 1 cut(s) 292
Sse9I AATT 2 cut(s) 80, 393
SsiI CCGC 4 cut(s) 154, 240, 387, 442
SspDI GGCGCC 1 cut(s) 290
StyD4I CCNGG 1 cut(s) 156
StyI CCWWGG 1 cut(s) 402
TaiI ACGT 3 cut(s) 57, 153, 171
TaqI TCGA 2 cut(s) 44, 103
TaqII GACCGA 1 cut(s) 439
TasI AATT 2 cut(s) 80, 393
TfiI GAWTC 1 cut(s) 88
TseFI GTSAC 2 cut(s) 162, 234
TseI GCWGC 1 cut(s) 373
Tsp45I GTSAC 2 cut(s) 162, 234
TspDTI ATGAA 2 cut(s) 62, 470
TspGWI ACGGA 3 cut(s) 38, 224, 387
Tth111I GACNNNGTC 1 cut(s) 326
XapI RAATTY 1 cut(s) 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.