pycom2247g00060

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00002247
OG0002208
Physical Location & Seq
Reverse (-)
27759 .. 29106
1348 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom2247g00060.1

Sequence Viewer

Length: 1144 bp
GAGAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAACCTGCGCAGCAGAACGACCCGAGAACCCGTTCCCACGTCGGGGGCCGCGGCCTTCGGGCTCGCGTCCTCTTATCCCGGGAGCCCGCTCCCGGCGTACAAACGAACACCGGCGCGTCTTTGCGCCAAGGAACCCGAACGAAAGAGCGCGCTCCGCCGCCCGGAAACGGTGCGCGCGCGGGCGGCGTCGTCGTCTTCTGTAAAGTCAAAACGACTCTCGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTGAATTGCAGAATCCCGTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAAGCCTTTAGGCCGAGGGCACGCCTGCCTGGGCGTCACACGCCGTTGCCCCCCCGCGCCTCCCTCGGGAGCGTCGGGGGGCGGACGATGGCCTCCCGTGCGTCGACCCGCGCGGTTGGCACAAATGCCGGGTGCTCGGCGACGAACGCCACGACAATCGGTGGTTGTCCAACCTCGGTTGCCGTTGTGCGCTTTCGTCGCGCTCCGAGCGGCCCGCGACGCTCCTCGCTCGGCTTCGGCCGAGCTTTCAACGCGACCCCAGGTCAGGCGGGGTTACCCGCTGAATTTAAGCATATCAATAAGCGGAGGAAAAGAAACTTACGAGGATTCCCCTAGTAACGGCGAGCGAACCGGGAACAGCCCAGCTAAATACGGGCGAGAGACCGATAGCAAACAAGTACCGCGAGGGAAAGATGAAAAGGACTTTGAAAAGAGAGTCAAAGAGTGCTTGAAATTGTCGGGAGGGAAGCGGATGGGGGCTGGCGATGCGCCCCGGTCGGATGTGGAACGGTCGAAAGCCGGTCCGCCGATCGGCTCGGGGCGTGGACCGACGCGGATTGCGGCGGCGGCCAAAGCCCGGGCTGTTGATATGCCCGCGGAGACGCCGTCGACGCGATCGTGGCGGGCAGCGCGCGCCGTACCGGCGTGCTTCGGCAACTGCGCGCTCCCGGCGTCGGCCAGCGAGCTCCCCATTCGGCCCGTCTTGAAACACGGACCAAGGAGTCTGACATGCGTGCGAGTCAACGGGCGATCAAACCCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

381

Amino Acids

39.65

Weight (kDa)

11.63

Isoelectric Point (pI)

50.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1102
Acc16I TGCGCA 1 cut(s) 60
Acc36I ACCTGC 2 cut(s) 41, 64
AccBSI CCGCTC 2 cut(s) 140, 591
AccI GTMKAC 2 cut(s) 485, 990
AclWI GGATC 1 cut(s) 50
AcoI YGGCCR 3 cut(s) 619, 949, 1057
AcsI RAATTY 1 cut(s) 665
AcyI GRCGYC 4 cut(s) 237, 415, 984, 1053
AfaI GTAC 3 cut(s) 150, 781, 1021
AgsI TTSAA 5 cut(s) 366, 631, 810, 833, 1088
AhdI GACNNNNNGTC 1 cut(s) 642
AjiI CACGTC 1 cut(s) 91
AjnI CCWGG 2 cut(s) 409, 640
AluBI AGCT 3 cut(s) 626, 747, 1067
AluI AGCT 3 cut(s) 626, 747, 1067
Alw21I GWGCWC 2 cut(s) 518, 1069
Alw26I GTCTC 2 cut(s) 756, 975
AlwI GGATC 1 cut(s) 50
Ama87I CYCGRG 5 cut(s) 73, 129, 446, 917, 958
AoxI GGCC 9 cut(s) 97, 103, 392, 471, 592, 619, 949, 1057, 1077
ApeKI GCWGC 2 cut(s) 61, 1008
ApoI RAATTY 1 cut(s) 665
Asp700I GAANNNNTTC 1 cut(s) 82
AspS9I GGNCC 6 cut(s) 97, 593, 903, 927, 1078, 1095
AsuHPI GGTGA 1 cut(s) 39
AvaI CYCGRG 5 cut(s) 73, 129, 446, 917, 958
AvaII GGWCC 3 cut(s) 903, 927, 1095
BaeGI GKGCMC 1 cut(s) 403
BanII GRGCYC 3 cut(s) 115, 138, 1069
BbsI GAAGAC 1 cut(s) 238
Bbv12I GWGCWC 2 cut(s) 518, 1069
BbvI GCAGC 2 cut(s) 73, 1020
BccI CCATC 3 cut(s) 361, 463, 848
BceAI ACGGC 5 cut(s) 409, 548, 737, 971, 1002
BciT130I CCWGG 2 cut(s) 411, 642
BcoDI GTCTC 2 cut(s) 756, 975
BfaI CTAG 1 cut(s) 715
BfuAI ACCTGC 2 cut(s) 41, 64
BglI GCCNNNNNGGC 1 cut(s) 1023
Bme18I GGWCC 3 cut(s) 903, 927, 1095
BmeRI GACNNNNNGTC 1 cut(s) 642
BmeT110I CYCGRG 5 cut(s) 73, 129, 446, 917, 958
BmgBI CACGTC 1 cut(s) 91
BmgT120I GGNCC 6 cut(s) 97, 593, 903, 927, 1078, 1095
BmiI GGNNCC 3 cut(s) 98, 135, 184
BmsI GCATC 2 cut(s) 303, 857
BpiI GAAGAC 1 cut(s) 238
Bsa29I ATCGAT 1 cut(s) 297
BsaHI GRCGYC 4 cut(s) 237, 415, 984, 1053
BsaI GGTCTC 1 cut(s) 756
BsaXI ACNNNNNCTCC 2 cut(s) 972, 1002
Bse118I RCCGGY 3 cut(s) 161, 900, 1022
BseBI CCWGG 2 cut(s) 411, 642
BseCI ATCGAT 1 cut(s) 297
BseGI GGATG 2 cut(s) 859, 887
BsePI GCGCGC 6 cut(s) 199, 224, 226, 1011, 1013, 1042
BseRI GAGGAG 1 cut(s) 595
BseSI GKGCMC 1 cut(s) 403
BseX3I CGGCCG 1 cut(s) 619
BseXI GCAGC 2 cut(s) 73, 1020
BseYI CCCAGC 1 cut(s) 743
Bsh1285I CGRYCG 5 cut(s) 622, 879, 894, 913, 999
BshFI GGCC 9 cut(s) 99, 105, 394, 473, 594, 621, 951, 1059, 1079
BshVI ATCGAT 1 cut(s) 297
BsiEI CGRYCG 5 cut(s) 622, 879, 894, 913, 999
BsiHKAI GWGCWC 2 cut(s) 518, 1069
BsiHKCI CYCGRG 5 cut(s) 73, 129, 446, 917, 958
BsmAI GTCTC 2 cut(s) 756, 975
BsmBI CGTCTC 1 cut(s) 975
BsnI GGCC 9 cut(s) 99, 105, 394, 473, 594, 621, 951, 1059, 1079
Bso31I GGTCTC 1 cut(s) 756
BsoBI CYCGRG 5 cut(s) 73, 129, 446, 917, 958
Bsp1286I GDGCHC 5 cut(s) 115, 138, 403, 518, 1069
Bsp143I GATC 4 cut(s) 42, 910, 996, 1131
BspANI GGCC 9 cut(s) 99, 105, 394, 473, 594, 621, 951, 1059, 1079
BspDI ATCGAT 1 cut(s) 297
BspLI GGNNCC 3 cut(s) 98, 135, 184
BspMI ACCTGC 2 cut(s) 41, 64
BspPI GGATC 1 cut(s) 50
BspTNI GGTCTC 1 cut(s) 756
BsrBI CCGCTC 2 cut(s) 140, 591
BsrFI RCCGGY 3 cut(s) 161, 900, 1022
BssAI RCCGGY 3 cut(s) 161, 900, 1022
BssHII GCGCGC 6 cut(s) 199, 224, 226, 1011, 1013, 1042
BssMI GATC 4 cut(s) 42, 910, 996, 1131
BssNI GRCGYC 4 cut(s) 237, 415, 984, 1053
BssT1I CCWWGG 2 cut(s) 178, 1098
Bst2UI CCWGG 2 cut(s) 411, 642
Bst4CI ACNGT 2 cut(s) 221, 892
BstACI GRCGYC 4 cut(s) 237, 415, 984, 1053
BstDSI CCRYGG 2 cut(s) 100, 976
BstEII GGTNACC 1 cut(s) 654
BstENI CCTNNNNNAGG 1 cut(s) 37
BstF5I GGATG 2 cut(s) 859, 887
BstKTI GATC 4 cut(s) 45, 913, 999, 1134
BstMAI GTCTC 2 cut(s) 756, 975
BstMBI GATC 4 cut(s) 42, 910, 996, 1131
BstMCI CGRYCG 5 cut(s) 622, 879, 894, 913, 999
BstNI CCWGG 2 cut(s) 411, 642
BstNSI RCATGY 1 cut(s) 1114
BstPI GGTNACC 1 cut(s) 654
BstSLI GKGCMC 1 cut(s) 403
BstV1I GCAGC 2 cut(s) 73, 1020
BstV2I GAAGAC 1 cut(s) 238
BstZI CGGCCG 1 cut(s) 619
Bsu15I ATCGAT 1 cut(s) 297
BsuRI GGCC 9 cut(s) 99, 105, 394, 473, 594, 621, 951, 1059, 1079
BsuTUI ATCGAT 1 cut(s) 297
BtgI CCRYGG 2 cut(s) 100, 976
BtgZI GCGATG 1 cut(s) 880
BtrI CACGTC 1 cut(s) 91
BtsCI GGATG 2 cut(s) 859, 887
BveI ACCTGC 2 cut(s) 41, 64
Cfr10I RCCGGY 3 cut(s) 161, 900, 1022
Cfr13I GGNCC 6 cut(s) 97, 593, 903, 927, 1078, 1095
Cfr42I CCGCGG 2 cut(s) 103, 979
Cfr9I CCCGGG 2 cut(s) 129, 958
ClaI ATCGAT 1 cut(s) 297
CpoI CGGWCCG 1 cut(s) 903
Csp6I GTAC 3 cut(s) 149, 780, 1020
CspI CGGWCCG 1 cut(s) 903
CviAII CATG 1 cut(s) 1111
CviQI GTAC 3 cut(s) 149, 780, 1020
DpnI GATC 4 cut(s) 44, 912, 998, 1133
DpnII GATC 4 cut(s) 42, 910, 996, 1131
DrdI GACNNNNNNGTC 1 cut(s) 1102
DriI GACNNNNNGTC 1 cut(s) 642
DseDI GACNNNNNNGTC 1 cut(s) 1102
EaeI YGGCCR 3 cut(s) 619, 949, 1057
EagI CGGCCG 1 cut(s) 619
Eam1105I GACNNNNNGTC 1 cut(s) 642
EciI GGCGGA 3 cut(s) 195, 478, 895
Ecl136II GAGCTC 1 cut(s) 1067
EclXI CGGCCG 1 cut(s) 619
Eco130I CCWWGG 2 cut(s) 178, 1098
Eco24I GRGCYC 3 cut(s) 115, 138, 1069
Eco31I GGTCTC 1 cut(s) 756
Eco32I GATATC 1 cut(s) 281
Eco47I GGWCC 3 cut(s) 903, 927, 1095
Eco52I CGGCCG 1 cut(s) 619
Eco53kI GAGCTC 1 cut(s) 1067
Eco88I CYCGRG 5 cut(s) 73, 129, 446, 917, 958
Eco91I GGTNACC 1 cut(s) 654
EcoICRI GAGCTC 1 cut(s) 1067
EcoNI CCTNNNNNAGG 1 cut(s) 37
EcoO65I GGTNACC 1 cut(s) 654
EcoRII CCWGG 2 cut(s) 409, 640
EcoRV GATATC 1 cut(s) 281
EcoT14I CCWWGG 2 cut(s) 178, 1098
EcoT38I GRGCYC 3 cut(s) 115, 138, 1069
ErhI CCWWGG 2 cut(s) 178, 1098
Esp3I CGTCTC 1 cut(s) 975
FaeI CATG 1 cut(s) 1114
FaiI YATR 3 cut(s) 675, 972, 1112
FatI CATG 1 cut(s) 1110
FauI CCCGC 9 cut(s) 145, 223, 443, 497, 603, 643, 667, 983, 997
FblI GTMKAC 2 cut(s) 485, 990
FokI GGATG 2 cut(s) 866, 894
FriOI GRGCYC 3 cut(s) 115, 138, 1069
FspBI CTAG 1 cut(s) 715
FspI TGCGCA 1 cut(s) 60
GsaI CCCAGC 1 cut(s) 747
HaeIII GGCC 9 cut(s) 99, 105, 394, 473, 594, 621, 951, 1059, 1079
Hin1I GRCGYC 4 cut(s) 237, 415, 984, 1053
Hin1II CATG 1 cut(s) 1114
HincII GTYRAC 3 cut(s) 486, 991, 1124
HindII GTYRAC 3 cut(s) 486, 991, 1124
HinfI GANTC 7 cut(s) 265, 341, 358, 708, 817, 1103, 1120
HphI GGTGA 1 cut(s) 39
Hpy166II GTNNAC 6 cut(s) 30, 350, 486, 927, 991, 1124
Hpy188I TCNGA 3 cut(s) 588, 881, 1108
Hpy188III TCNNGA 3 cut(s) 448, 841, 1085
Hpy8I GTNNAC 6 cut(s) 30, 350, 486, 927, 991, 1124
HpyAV CCTTC 2 cut(s) 33, 116
HpyCH4III ACNGT 2 cut(s) 221, 892
HpyCH4IV ACGT 2 cut(s) 90, 307
HpyCH4V TGCA 1 cut(s) 338
HpySE526I ACGT 2 cut(s) 90, 307
Hsp92I GRCGYC 4 cut(s) 237, 415, 984, 1053
Hsp92II CATG 1 cut(s) 1114
KspI CCGCGG 2 cut(s) 103, 979
Kzo9I GATC 4 cut(s) 42, 910, 996, 1131
LmnI GCTCC 8 cut(s) 133, 145, 208, 450, 589, 608, 1051, 1072
Lsp1109I GCAGC 2 cut(s) 73, 1020
LweI GCATC 2 cut(s) 303, 857
MaeI CTAG 1 cut(s) 715
MaeII ACGT 2 cut(s) 90, 307
MaeIII GTNAC 4 cut(s) 9, 416, 654, 717
MalI GATC 4 cut(s) 44, 912, 998, 1133
MauBI CGCGCGCG 1 cut(s) 226
MbiI CCGCTC 2 cut(s) 140, 591
MboI GATC 4 cut(s) 42, 910, 996, 1131
MboII GAAGA 2 cut(s) 238, 314
MhlI GDGCHC 5 cut(s) 115, 138, 403, 518, 1069
MluCI AATT 3 cut(s) 333, 665, 834
MlyI GAGTC 5 cut(s) 259, 367, 826, 1112, 1129
MmeI TCCRAC 2 cut(s) 575, 859
MroXI GAANNNNTTC 1 cut(s) 82
MseI TTAA 1 cut(s) 669
MspA1I CMGCKG 3 cut(s) 102, 662, 978
MvaI CCWGG 2 cut(s) 411, 642
NdeII GATC 4 cut(s) 42, 910, 996, 1131
NlaIII CATG 1 cut(s) 1114
NlaIV GGNNCC 3 cut(s) 98, 135, 184
NmeAIII GCCGAG 6 cut(s) 249, 264, 420, 497, 591, 647
NmuCI GTSAC 1 cut(s) 416
NsbI TGCGCA 1 cut(s) 60
NspI RCATGY 1 cut(s) 1114
PauI GCGCGC 6 cut(s) 199, 224, 226, 1011, 1013, 1042
PcsI WCGNNNNNNNCGW 2 cut(s) 530, 996
PdmI GAANNNNTTC 1 cut(s) 82
PfeI GAWTC 2 cut(s) 341, 708
PflFI GACNNNGTC 1 cut(s) 986
Ple19I CGATCG 2 cut(s) 913, 999
PleI GAGTC 5 cut(s) 259, 366, 825, 1111, 1128
PpsI GAGTC 5 cut(s) 259, 366, 825, 1111, 1128
Psp124BI GAGCTC 1 cut(s) 1069
Psp6I CCWGG 2 cut(s) 409, 640
PspEI GGTNACC 1 cut(s) 654
PspFI CCCAGC 1 cut(s) 743
PspGI CCWGG 2 cut(s) 409, 640
PspN4I GGNNCC 3 cut(s) 98, 135, 184
PspPI GGNCC 6 cut(s) 97, 593, 903, 927, 1078, 1095
PsyI GACNNNGTC 1 cut(s) 986
PteI GCGCGC 6 cut(s) 199, 224, 226, 1011, 1013, 1042
PvuI CGATCG 2 cut(s) 913, 999
RsaI GTAC 3 cut(s) 150, 781, 1021
RsaNI GTAC 3 cut(s) 149, 780, 1020
Rsr2I CGGWCCG 1 cut(s) 903
RsrII CGGWCCG 1 cut(s) 903
SacI GAGCTC 1 cut(s) 1069
SacII CCGCGG 2 cut(s) 103, 979
SalI GTCGAC 2 cut(s) 484, 989
SaqAI TTAA 1 cut(s) 669
Sau3AI GATC 4 cut(s) 42, 910, 996, 1131
Sau96I GGNCC 6 cut(s) 97, 593, 903, 927, 1078, 1095
SchI GAGTC 5 cut(s) 259, 367, 826, 1112, 1129
SduI GDGCHC 5 cut(s) 115, 138, 403, 518, 1069
SfaNI GCATC 2 cut(s) 303, 857
Sfr303I CCGCGG 2 cut(s) 103, 979
SgrAI CRCCGGYG 1 cut(s) 161
SgrBI CCGCGG 2 cut(s) 103, 979
SgrDI CGTCGACG 1 cut(s) 989
SinI GGWCC 3 cut(s) 903, 927, 1095
SmaI CCCGGG 2 cut(s) 131, 960
SrfI GCCCGGGC 1 cut(s) 960
Sse9I AATT 3 cut(s) 333, 665, 834
SspMI CTAG 1 cut(s) 715
SstI GAGCTC 1 cut(s) 1069
StyI CCWWGG 2 cut(s) 178, 1098
TaaI ACNGT 2 cut(s) 221, 892
TaiI ACGT 2 cut(s) 93, 310
TaqI TCGA 6 cut(s) 51, 297, 356, 485, 894, 990
TaqII GACCGA 2 cut(s) 780, 944
TasI AATT 3 cut(s) 333, 665, 834
TauI GCSGC 8 cut(s) 102, 105, 211, 237, 594, 945, 948, 951
TfiI GAWTC 2 cut(s) 341, 708
Tru1I TTAA 1 cut(s) 669
Tru9I TTAA 1 cut(s) 669
TseFI GTSAC 1 cut(s) 416
TseI GCWGC 2 cut(s) 61, 1008
Tsp45I GTSAC 1 cut(s) 416
TspDTI ATGAA 2 cut(s) 315, 811
TspGWI ACGGA 3 cut(s) 11, 291, 1108
TspMI CCCGGG 2 cut(s) 129, 958
Tth111I GACNNNGTC 1 cut(s) 986
VpaK11BI GGWCC 3 cut(s) 903, 927, 1095
XagI CCTNNNNNAGG 1 cut(s) 37
XapI RAATTY 1 cut(s) 665
XceI RCATGY 1 cut(s) 1114
XmaI CCCGGG 2 cut(s) 129, 958
XmiI GTMKAC 2 cut(s) 485, 990
XmnI GAANNNNTTC 1 cut(s) 82
XspI CTAG 1 cut(s) 715
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.