pycom553g00010

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_553
OG0002208
Physical Location & Seq
Forward (+)
475 .. 2660
2186 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom553g00010.6

Sequence Viewer

Length: 1452 bp
ATGAGTGGTAATTGGGTTGTATTCGACGGCAGGCTCCGTGCTTCGGCATCGAACTGTCGGCTCGTACCCCGCTTCATCGACGCGTCGAGATTCGTCTCGTGCGCAGGATGGGTTTCTGTTTTGGCTACCTGCTGCGAAGGAACGTTGCCTCTCGTCGTCCCTTTCCTCCTCCTGCCGGCCTTGCCGGCGGGAGGACGACATCGTGGCGGTGCTCGTGTCCCGGACGAGCCGCACTCGTTGCGGGGCGGTGGGTCTTCACGACCTCCGATCGTCCGATCGAAGCCTTGTCGCTCGACGCGTACGACCGTCGCGCCCGCTGCGAGCCGTCGACCGTCAAGGTCGTCCGTCTCGCGCGACGCCGACGTCGAAGAGGAATGCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATTTATAGTTTGTTTGATGGTATCTGCTACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAACAAACCCCGACTTCTGGAAGGGACGCATTTATTAGATAAAAGGTCGACGCGGGCTCTGCCCGTTGCTCTGATGATTCATGATAACTCGACGGATCGCACAGCCATCGTGCTGGCGACGCATCATTCAAATATCTCTCGTAGTTGGACCTTGGGTTGGGTCGACCGGTCCGCCTCTGGTGTGCACCGGTCGGCTCGTCCCTTCTGCCGGCGATACGCTCCTGGCCTTAATTGGCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGATTTCGGTCCTATTCTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCGACGAACAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGCTCGAAGATCGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGGCGGATGTTACTTTTAGGACTCCGCCGGCACCTTATGAGAAATCAAAGTTTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGGTCATCCTCCGCGGCCAGCTTCTTAGAGGGACTATGGCCATCATTGCAATTGTTGGTCTTCAACGAGGAATTCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTCCTACCGATTGAATGGTCCGGTGAAGTGTTCGGATCGCGGCGACGCGGGTGGTTCGCTGCCCGCGACGTCGCGAGAAGTCCACTGAACCTTATCATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

484

Amino Acids

52.42

Weight (kDa)

10.01

Isoelectric Point (pI)

54.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 362
AatII GACGTC 2 cut(s) 366, 1422
Acc16I TGCGCA 1 cut(s) 103
Acc36I ACCTGC 1 cut(s) 137
AccB1I GGYRCC 2 cut(s) 994, 1075
AccI GTMKAC 4 cut(s) 328, 545, 660, 844
AclI AACGTT 1 cut(s) 143
AclWI GGATC 5 cut(s) 383, 600, 824, 970, 1393
AcoI YGGCCR 4 cut(s) 731, 1130, 1225, 1248
AcsI RAATTY 1 cut(s) 1281
AcyI GRCGYC 3 cut(s) 357, 363, 1419
AfaI GTAC 3 cut(s) 66, 301, 1336
AfiI CCNNNNNNNGG 7 cut(s) 43, 175, 191, 514, 654, 705, 964
AflIII ACRYGT 2 cut(s) 81, 296
AgeI ACCGGT 2 cut(s) 663, 684
AgsI TTSAA 4 cut(s) 627, 763, 1274, 1364
AjnI CCWGG 4 cut(s) 381, 718, 957, 1081
AjuI GAANNNNNNNTTGG 2 cut(s) 863, 895
AluBI AGCT 4 cut(s) 488, 1203, 1231, 1307
AluI AGCT 4 cut(s) 488, 1203, 1231, 1307
Alw21I GWGCWC 3 cut(s) 214, 684, 779
Alw26I GTCTC 5 cut(s) 100, 352, 418, 940, 1176
Alw44I GTGCAC 1 cut(s) 680
AlwI GGATC 5 cut(s) 383, 600, 824, 970, 1393
AoxI GGCC 7 cut(s) 177, 721, 731, 807, 1130, 1225, 1248
ApaLI GTGCAC 1 cut(s) 680
ApeKI GCWGC 3 cut(s) 132, 317, 1409
ApoI RAATTY 1 cut(s) 1281
AseI ATTAAT 1 cut(s) 885
AsiGI ACCGGT 2 cut(s) 663, 684
AspLEI GCGC 6 cut(s) 104, 313, 354, 752, 1099, 1296
AspS9I GGNCC 4 cut(s) 645, 666, 794, 1368
AsuC2I CCSGG 3 cut(s) 221, 735, 1027
AsuHPI GGTGA 1 cut(s) 1385
AvaII GGWCC 4 cut(s) 645, 666, 794, 1368
BaeGI GKGCMC 2 cut(s) 684, 1078
BalI TGGCCA 1 cut(s) 1250
BanI GGYRCC 2 cut(s) 994, 1075
BanII GRGCYC 2 cut(s) 556, 909
BauI CACGAG 2 cut(s) 97, 213
BbsI GAAGAC 2 cut(s) 246, 1262
Bbv12I GWGCWC 3 cut(s) 214, 684, 779
BbvCI CCTCAGC 1 cut(s) 1187
BbvI GCAGC 3 cut(s) 119, 304, 1396
BccI CCATC 4 cut(s) 102, 443, 611, 1259
BceAI ACGGC 3 cut(s) 43, 309, 1117
BcgI CGANNNNNNTGC 2 cut(s) 586, 620
BciT130I CCWGG 4 cut(s) 383, 720, 959, 1083
BcnI CCSGG 3 cut(s) 221, 735, 1027
BcoDI GTCTC 5 cut(s) 100, 352, 418, 940, 1176
BfaI CTAG 4 cut(s) 483, 932, 1200, 1287
BfoI RGCGCY 1 cut(s) 753
BfuAI ACCTGC 1 cut(s) 137
BglI GCCNNNNNGGC 1 cut(s) 185
BisI GCNGC 6 cut(s) 133, 230, 318, 1225, 1391, 1410
BlsI GCNGC 6 cut(s) 134, 231, 319, 1226, 1392, 1411
Bme1390I CCNGG 7 cut(s) 221, 383, 720, 735, 959, 1027, 1083
Bme18I GGWCC 4 cut(s) 645, 666, 794, 1368
BmgT120I GGNCC 4 cut(s) 645, 666, 794, 1368
BmiI GGNNCC 5 cut(s) 35, 996, 1024, 1077, 1092
BmrFI CCNGG 7 cut(s) 221, 383, 720, 735, 959, 1027, 1083
BmsI GCATC 3 cut(s) 56, 628, 940
BpiI GAAGAC 2 cut(s) 246, 1262
BplI GAGNNNNNCTC 2 cut(s) 218, 250
Bpu10I CCTNAGC 1 cut(s) 1187
BpuMI CCSGG 3 cut(s) 221, 735, 1027
Bsa29I ATCGAT 1 cut(s) 916
BsaAI YACGTR 1 cut(s) 495
BsaHI GRCGYC 3 cut(s) 357, 363, 1419
BsaI GGTCTC 1 cut(s) 1176
BsaJI CCNNGG 5 cut(s) 648, 870, 958, 1026, 1222
BsaWI WCCGGW 3 cut(s) 663, 684, 1370
Bsc4I CCNNNNNNNGG 7 cut(s) 43, 175, 191, 514, 654, 705, 964
Bse118I RCCGGY 6 cut(s) 175, 184, 663, 684, 705, 991
Bse1I ACTGG 1 cut(s) 396
Bse3DI GCAATG 1 cut(s) 1254
BseBI CCWGG 4 cut(s) 383, 720, 959, 1083
BseCI ATCGAT 1 cut(s) 916
BseDI CCNNGG 5 cut(s) 648, 870, 958, 1026, 1222
BseGI GGATG 4 cut(s) 113, 880, 976, 1215
BseLI CCNNNNNNNGG 7 cut(s) 43, 175, 191, 514, 654, 705, 964
BseMI GCAATG 1 cut(s) 1254
BseMII CTCAG 1 cut(s) 1201
BseNI ACTGG 1 cut(s) 396
BseRI GAGGAG 1 cut(s) 158
BseSI GKGCMC 2 cut(s) 684, 1078
BseXI GCAGC 3 cut(s) 119, 304, 1396
Bsh1285I CGRYCG 6 cut(s) 270, 278, 306, 332, 664, 689
BshFI GGCC 7 cut(s) 179, 723, 733, 809, 1132, 1227, 1250
BshNI GGYRCC 2 cut(s) 994, 1075
BshTI ACCGGT 2 cut(s) 663, 684
BshVI ATCGAT 1 cut(s) 916
BsiEI CGRYCG 6 cut(s) 270, 278, 306, 332, 664, 689
BsiHKAI GWGCWC 3 cut(s) 214, 684, 779
BsiWI CGTACG 1 cut(s) 299
BslFI GGGAC 7 cut(s) 143, 203, 535, 681, 851, 1255, 1307
BslI CCNNNNNNNGG 7 cut(s) 43, 175, 191, 514, 654, 705, 964
BsmAI GTCTC 5 cut(s) 100, 352, 418, 940, 1176
BsmBI CGTCTC 2 cut(s) 100, 352
BsmFI GGGAC 7 cut(s) 143, 203, 535, 681, 851, 1255, 1307
BsmI GAATGC 1 cut(s) 380
BsnI GGCC 7 cut(s) 179, 723, 733, 809, 1132, 1227, 1250
Bso31I GGTCTC 1 cut(s) 1176
Bsp1286I GDGCHC 6 cut(s) 214, 556, 684, 779, 909, 1078
Bsp1407I TGTACA 1 cut(s) 1334
Bsp143I GATC 9 cut(s) 267, 275, 388, 592, 816, 913, 917, 962, 1385
Bsp68I TCGCGA 1 cut(s) 1424
BspANI GGCC 7 cut(s) 179, 723, 733, 809, 1132, 1227, 1250
BspCNI CTCAG 1 cut(s) 1200
BspDI ATCGAT 1 cut(s) 916
BspHI TCATGA 1 cut(s) 577
BspLI GGNNCC 5 cut(s) 35, 996, 1024, 1077, 1092
BspMI ACCTGC 1 cut(s) 137
BspPI GGATC 5 cut(s) 383, 600, 824, 970, 1393
BspT107I GGYRCC 2 cut(s) 994, 1075
BspTNI GGTCTC 1 cut(s) 1176
BsrDI GCAATG 1 cut(s) 1254
BsrFI RCCGGY 6 cut(s) 175, 184, 663, 684, 705, 991
BsrGI TGTACA 1 cut(s) 1334
BsrI ACTGG 1 cut(s) 396
BssAI RCCGGY 6 cut(s) 175, 184, 663, 684, 705, 991
BssECI CCNNGG 5 cut(s) 648, 870, 958, 1026, 1222
BssMI GATC 9 cut(s) 267, 275, 388, 592, 816, 913, 917, 962, 1385
BssNI GRCGYC 3 cut(s) 357, 363, 1419
BssSI CACGAG 2 cut(s) 97, 213
BssT1I CCWWGG 2 cut(s) 648, 870
Bst2BI CACGAG 2 cut(s) 97, 213
Bst2UI CCWGG 4 cut(s) 383, 720, 959, 1083
Bst4CI ACNGT 6 cut(s) 56, 307, 333, 473, 842, 928
Bst6I CTCTTC 1 cut(s) 363
BstACI GRCGYC 3 cut(s) 357, 363, 1419
BstAPI GCANNNNNTGC 1 cut(s) 238
BstAUI TGTACA 1 cut(s) 1334
BstBAI YACGTR 1 cut(s) 495
BstDEI CTNAG 3 cut(s) 1138, 1187, 1235
BstDSI CCRYGG 1 cut(s) 1222
BstF5I GGATG 4 cut(s) 113, 880, 976, 1215
BstH2I RGCGCY 1 cut(s) 753
BstHHI GCGC 6 cut(s) 104, 313, 354, 752, 1099, 1296
BstKTI GATC 9 cut(s) 270, 278, 391, 595, 819, 916, 920, 965, 1388
BstMAI GTCTC 5 cut(s) 100, 352, 418, 940, 1176
BstMBI GATC 9 cut(s) 267, 275, 388, 592, 816, 913, 917, 962, 1385
BstMCI CGRYCG 6 cut(s) 270, 278, 306, 332, 664, 689
BstMWI GCNNNNNNNGC 8 cut(s) 181, 185, 238, 317, 556, 616, 749, 1256
BstNI CCWGG 4 cut(s) 383, 720, 959, 1083
BstSCI CCNGG 7 cut(s) 219, 381, 718, 733, 957, 1025, 1081
BstSLI GKGCMC 2 cut(s) 684, 1078
BstV1I GCAGC 3 cut(s) 119, 304, 1396
BstV2I GAAGAC 2 cut(s) 246, 1262
BstXI CCANNNNNNTGG 1 cut(s) 610
Bsu15I ATCGAT 1 cut(s) 916
BsuRI GGCC 7 cut(s) 179, 723, 733, 809, 1132, 1227, 1250
BsuTUI ATCGAT 1 cut(s) 916
BtgI CCRYGG 1 cut(s) 1222
BtsCI GGATG 4 cut(s) 113, 880, 976, 1215
BtsIMutI CAGTG 1 cut(s) 1433
BtuMI TCGCGA 1 cut(s) 1424
BveI ACCTGC 1 cut(s) 137
CciI TCATGA 1 cut(s) 577
CfoI GCGC 6 cut(s) 104, 313, 354, 752, 1099, 1296
Cfr10I RCCGGY 6 cut(s) 175, 184, 663, 684, 705, 991
Cfr13I GGNCC 4 cut(s) 645, 666, 794, 1368
Cfr42I CCGCGG 1 cut(s) 1225
ClaI ATCGAT 1 cut(s) 916
CpoI CGGWCCG 1 cut(s) 666
CseI GACGC 8 cut(s) 72, 89, 304, 365, 532, 556, 625, 1404
CsiI ACCWGGT 1 cut(s) 381
Csp6I GTAC 3 cut(s) 65, 300, 1335
CspAI ACCGGT 2 cut(s) 663, 684
CspI CGGWCCG 1 cut(s) 666
CviAII CATG 3 cut(s) 429, 578, 1128
CviQI GTAC 3 cut(s) 65, 300, 1335
DdeI CTNAG 3 cut(s) 1138, 1187, 1235
DpnI GATC 9 cut(s) 269, 277, 390, 594, 818, 915, 919, 964, 1387
DpnII GATC 9 cut(s) 267, 275, 388, 592, 816, 913, 917, 962, 1385
DrdI GACNNNNNNGTC 1 cut(s) 362
DseDI GACNNNNNNGTC 1 cut(s) 362
EaeI YGGCCR 4 cut(s) 731, 1130, 1225, 1248
Eam1104I CTCTTC 1 cut(s) 363
EarI CTCTTC 1 cut(s) 363
EciI GGCGGA 3 cut(s) 658, 978, 983
Eco130I CCWWGG 2 cut(s) 648, 870
Eco24I GRGCYC 2 cut(s) 556, 909
Eco31I GGTCTC 1 cut(s) 1176
Eco47I GGWCC 4 cut(s) 645, 666, 794, 1368
EcoRI GAATTC 1 cut(s) 1281
EcoRII CCWGG 4 cut(s) 381, 718, 957, 1081
EcoT14I CCWWGG 2 cut(s) 648, 870
EcoT22I ATGCAT 1 cut(s) 434
EcoT38I GRGCYC 2 cut(s) 556, 909
ErhI CCWWGG 2 cut(s) 648, 870
Esp3I CGTCTC 2 cut(s) 100, 352
FaeI CATG 3 cut(s) 432, 581, 1131
FaqI GGGAC 7 cut(s) 143, 203, 535, 681, 851, 1255, 1307
FatI CATG 3 cut(s) 428, 577, 1127
FauI CCCGC 7 cut(s) 77, 181, 234, 322, 543, 1391, 1421
FauNDI CATATG 1 cut(s) 405
FblI GTMKAC 4 cut(s) 328, 545, 660, 844
Fnu4HI GCNGC 6 cut(s) 133, 230, 318, 1225, 1391, 1410
FokI GGATG 4 cut(s) 120, 887, 983, 1202
FriOI GRGCYC 2 cut(s) 556, 909
Fsp4HI GCNGC 6 cut(s) 133, 230, 318, 1225, 1391, 1410
FspBI CTAG 4 cut(s) 483, 932, 1200, 1287
FspI TGCGCA 1 cut(s) 103
GlaI GCGC 6 cut(s) 103, 312, 353, 751, 1098, 1295
GluI GCNGC 6 cut(s) 133, 230, 318, 1225, 1391, 1410
HaeII RGCGCY 1 cut(s) 753
HaeIII GGCC 7 cut(s) 179, 723, 733, 809, 1132, 1227, 1250
HgaI GACGC 8 cut(s) 72, 89, 304, 365, 532, 556, 625, 1404
HhaI GCGC 6 cut(s) 104, 313, 354, 752, 1099, 1296
Hin1I GRCGYC 3 cut(s) 357, 363, 1419
Hin1II CATG 3 cut(s) 432, 581, 1131
Hin6I GCGC 6 cut(s) 102, 311, 352, 750, 1097, 1294
HinP1I GCGC 6 cut(s) 102, 311, 352, 750, 1097, 1294
HincII GTYRAC 6 cut(s) 329, 546, 661, 845, 1174, 1315
HindII GTYRAC 6 cut(s) 329, 546, 661, 845, 1174, 1315
HinfI GANTC 5 cut(s) 90, 574, 985, 1109, 1298
HpaI GTTAAC 1 cut(s) 1174
HphI GGTGA 1 cut(s) 1385
Hpy166II GTNNAC 9 cut(s) 329, 546, 661, 682, 845, 1174, 1315, 1337, 1433
Hpy188I TCNGA 7 cut(s) 267, 275, 466, 570, 821, 922, 1385
Hpy188III TCNNGA 9 cut(s) 87, 258, 483, 515, 578, 814, 890, 1106, 1423
Hpy8I GTNNAC 9 cut(s) 329, 546, 661, 682, 845, 1174, 1315, 1337, 1433
HpyAV CCTTC 5 cut(s) 131, 512, 709, 820, 1065
HpyCH4III ACNGT 6 cut(s) 56, 307, 333, 473, 842, 928
HpyCH4IV ACGT 5 cut(s) 143, 363, 494, 1320, 1419
HpyCH4V TGCA 5 cut(s) 432, 498, 682, 1127, 1259
HpyF10VI GCNNNNNNNGC 8 cut(s) 181, 185, 238, 317, 556, 616, 749, 1256
HpyF3I CTNAG 3 cut(s) 1138, 1187, 1235
HpySE526I ACGT 5 cut(s) 143, 363, 494, 1320, 1419
Hsp92I GRCGYC 3 cut(s) 357, 363, 1419
Hsp92II CATG 3 cut(s) 432, 581, 1131
HspAI GCGC 6 cut(s) 102, 311, 352, 750, 1097, 1294
KroI GCCGGC 4 cut(s) 175, 184, 705, 991
KroNI GCCGGC 4 cut(s) 177, 186, 707, 993
KspAI GTTAAC 1 cut(s) 1174
KspI CCGCGG 1 cut(s) 1225
Kzo9I GATC 9 cut(s) 267, 275, 388, 592, 816, 913, 917, 962, 1385
LmnI GCTCC 5 cut(s) 39, 721, 1090, 1148, 1356
Lsp1109I GCAGC 3 cut(s) 119, 304, 1396
LweI GCATC 3 cut(s) 56, 628, 940
MabI ACCWGGT 1 cut(s) 381
MaeI CTAG 4 cut(s) 483, 932, 1200, 1287
MaeII ACGT 5 cut(s) 143, 363, 494, 1320, 1419
MaeIII GTNAC 2 cut(s) 754, 973
MalI GATC 9 cut(s) 269, 277, 390, 594, 818, 915, 919, 964, 1387
MboI GATC 9 cut(s) 267, 275, 388, 592, 816, 913, 917, 962, 1385
MboII GAAGA 5 cut(s) 246, 380, 775, 923, 1262
MfeI CAATTG 1 cut(s) 1260
MhlI GDGCHC 6 cut(s) 214, 556, 684, 779, 909, 1078
MlsI TGGCCA 1 cut(s) 1250
MluCI AATT 8 cut(s) 10, 478, 727, 767, 1062, 1165, 1260, 1281
MluI ACGCGT 2 cut(s) 81, 296
MluNI TGGCCA 1 cut(s) 1250
MlyI GAGTC 2 cut(s) 979, 1307
MmeI TCCRAC 1 cut(s) 623
Mox20I TGGCCA 1 cut(s) 1250
Mph1103I ATGCAT 1 cut(s) 434
MroNI GCCGGC 4 cut(s) 175, 184, 705, 991
MscI TGGCCA 1 cut(s) 1250
MseI TTAA 7 cut(s) 423, 726, 831, 885, 1056, 1164, 1173
Msp20I TGGCCA 1 cut(s) 1250
MspA1I CMGCKG 2 cut(s) 317, 1224
MspR9I CCNGG 7 cut(s) 221, 383, 720, 735, 959, 1027, 1083
MunI CAATTG 1 cut(s) 1260
Mva1269I GAATGC 1 cut(s) 380
MvaI CCWGG 4 cut(s) 383, 720, 959, 1083
MwoI GCNNNNNNNGC 8 cut(s) 181, 185, 238, 317, 556, 616, 749, 1256
NaeI GCCGGC 4 cut(s) 177, 186, 707, 993
NciI CCSGG 3 cut(s) 221, 735, 1027
NdeI CATATG 1 cut(s) 405
NdeII GATC 9 cut(s) 267, 275, 388, 592, 816, 913, 917, 962, 1385
NgoMIV GCCGGC 4 cut(s) 175, 184, 705, 991
NlaIII CATG 3 cut(s) 432, 581, 1131
NlaIV GGNNCC 5 cut(s) 35, 996, 1024, 1077, 1092
NruI TCGCGA 1 cut(s) 1424
NsbI TGCGCA 1 cut(s) 103
NsiI ATGCAT 1 cut(s) 434
PagI TCATGA 1 cut(s) 577
PcsI WCGNNNNNNNCGW 5 cut(s) 295, 299, 363, 1355, 1413
PctI GAATGC 1 cut(s) 380
PdiI GCCGGC 4 cut(s) 177, 186, 707, 993
PfeI GAWTC 3 cut(s) 90, 574, 1109
Pfl23II CGTACG 1 cut(s) 299
PflFI GACNNNGTC 1 cut(s) 1319
PfoI TCCNGGA 1 cut(s) 219
PinAI ACCGGT 2 cut(s) 663, 684
PkrI GCNGC 6 cut(s) 134, 231, 319, 1226, 1392, 1411
Ple19I CGATCG 2 cut(s) 270, 278
PleI GAGTC 2 cut(s) 979, 1306
PpsI GAGTC 2 cut(s) 979, 1306
Ppu21I YACGTR 1 cut(s) 495
PshBI ATTAAT 1 cut(s) 885
Psp1406I AACGTT 1 cut(s) 143
Psp6I CCWGG 4 cut(s) 381, 718, 957, 1081
PspGI CCWGG 4 cut(s) 381, 718, 957, 1081
PspLI CGTACG 1 cut(s) 299
PspN4I GGNNCC 5 cut(s) 35, 996, 1024, 1077, 1092
PspPI GGNCC 4 cut(s) 645, 666, 794, 1368
PsyI GACNNNGTC 1 cut(s) 1319
PvuI CGATCG 2 cut(s) 270, 278
RruI TCGCGA 1 cut(s) 1424
RsaI GTAC 3 cut(s) 66, 301, 1336
RsaNI GTAC 3 cut(s) 65, 300, 1335
Rsr2I CGGWCCG 1 cut(s) 666
RsrII CGGWCCG 1 cut(s) 666
SacII CCGCGG 1 cut(s) 1225
SalI GTCGAC 4 cut(s) 327, 544, 659, 843
SaqAI TTAA 7 cut(s) 423, 726, 831, 885, 1056, 1164, 1173
SatI GCNGC 6 cut(s) 133, 230, 318, 1225, 1391, 1410
Sau3AI GATC 9 cut(s) 267, 275, 388, 592, 816, 913, 917, 962, 1385
Sau96I GGNCC 4 cut(s) 645, 666, 794, 1368
SchI GAGTC 2 cut(s) 979, 1307
ScrFI CCNGG 7 cut(s) 221, 383, 720, 735, 959, 1027, 1083
SduI GDGCHC 6 cut(s) 214, 556, 684, 779, 909, 1078
SexAI ACCWGGT 1 cut(s) 381
SfaNI GCATC 3 cut(s) 56, 628, 940
Sfr303I CCGCGG 1 cut(s) 1225
SgrBI CCGCGG 1 cut(s) 1225
SinI GGWCC 4 cut(s) 645, 666, 794, 1368
Sse9I AATT 8 cut(s) 10, 478, 727, 767, 1062, 1165, 1260, 1281
SspMI CTAG 4 cut(s) 483, 932, 1200, 1287
StyD4I CCNGG 7 cut(s) 219, 381, 718, 733, 957, 1025, 1081
StyI CCWWGG 2 cut(s) 648, 870
TaaI ACNGT 6 cut(s) 56, 307, 333, 473, 842, 928
TaiI ACGT 5 cut(s) 146, 366, 497, 1323, 1422
TaqII GACCGA 1 cut(s) 782
TasI AATT 8 cut(s) 10, 478, 727, 767, 1062, 1165, 1260, 1281
TatI WGTACW 1 cut(s) 1334
TauI GCSGC 3 cut(s) 232, 1227, 1393
TfiI GAWTC 3 cut(s) 90, 574, 1109
Tru1I TTAA 7 cut(s) 423, 726, 831, 885, 1056, 1164, 1173
Tru9I TTAA 7 cut(s) 423, 726, 831, 885, 1056, 1164, 1173
TscAI CASTG 1 cut(s) 1440
TseI GCWGC 3 cut(s) 132, 317, 1409
TspDTI ATGAA 3 cut(s) 64, 566, 871
TspGWI ACGGA 5 cut(s) 26, 334, 605, 1083, 1159
TspRI CASTG 1 cut(s) 1440
Tth111I GACNNNGTC 1 cut(s) 1319
VneI GTGCAC 1 cut(s) 680
VpaK11BI GGWCC 4 cut(s) 645, 666, 794, 1368
VspI ATTAAT 1 cut(s) 885
XapI RAATTY 1 cut(s) 1281
XbaI TCTAGA 1 cut(s) 482
XmiI GTMKAC 4 cut(s) 328, 545, 660, 844
XspI CTAG 4 cut(s) 483, 932, 1200, 1287
ZraI GACGTC 2 cut(s) 364, 1420
Zsp2I ATGCAT 1 cut(s) 434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.