pycom2246g00040

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00002246
OG0002208
Physical Location & Seq
Forward (+)
3551 .. 4800
1250 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom2246g00040.1

Sequence Viewer

Length: 1110 bp
ATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGGTCATCCTCCGCGGCCAGCTTCTTAGAGGGACTATGGCCGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTCTATAGCCTTGAGATCATTGCAATTGTTGGTCTTCAACGAGGAATTCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTCCTACCGATTGAATGGTCCGGTGAAGTGTTCGGATCGCGCATTTAGAGGAAGGAGAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAACCTGCGCAGCAGAACGACCCGAGAACCCGTTCCCACGTCGGGGGCCGGCGGGCCTTCGGGCTCGCGTCCCTTATCCCGGGAGCCCGCTCCCGGGCGTACAACGAACACCGGCGCGTCTTTGCGCCAAGGAACCCGAACGAAAGAGCGCGCTCCCGCCGCCCCGGAAACGGTGCGCGCGCGGGCGGCGTCGTCGTCTTCTGTAAAGTCAAAACGACTCTCGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTGAATTGCAGAATCCCGTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAAGCCTTTAGGCCGAGGGCACGCCTGCCTGGGCGTCACACGCCGTTGCCCCCCGCGCCTCCCTCGGGAGCGTCGGGGGGCGGACGATGGCCTCCCGTGCGTCGACCCGCGCGGTTGGCACAAATGCCGGGTGCTCGGCGACGAACGCCACGACAATCGGTGGTTGTCCAACCTCGGTTGCCCGTTGTGCGCTTTCGTCGCGCTCCGAGCGGCCCGCGACGCTCCTCGCTCGGCTTCGGCCGAGCTTTCAACGCGACCCCAGGTCAGGCGGGGTTACCCGCTGAATTTAAGCATATCAATAAGCGGAGGAAAAGAAACTTACGAGGATTCCCCTAGTAACGGCGAGCGAACCGGGAACAGCCCAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

370

Amino Acids

40.04

Weight (kDa)

10.67

Isoelectric Point (pI)

54.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 416
Acc36I ACCTGC 2 cut(s) 397, 420
AccBSI CCGCTC 2 cut(s) 497, 950
AccI GTMKAC 1 cut(s) 843
AclWI GGATC 2 cut(s) 345, 406
AcoI YGGCCR 4 cut(s) 17, 112, 135, 978
AcsI RAATTY 2 cut(s) 233, 1024
AcyI GRCGYC 2 cut(s) 596, 774
AfaI GTAC 2 cut(s) 288, 508
AgsI TTSAA 5 cut(s) 184, 226, 316, 725, 990
AhdI GACNNNNNGTC 1 cut(s) 1001
AjiI CACGTC 1 cut(s) 447
AjnI CCWGG 2 cut(s) 768, 999
AluBI AGCT 5 cut(s) 90, 118, 259, 985, 1106
AluI AGCT 5 cut(s) 90, 118, 259, 985, 1106
Alw21I GWGCWC 1 cut(s) 876
Alw26I GTCTC 1 cut(s) 63
AlwI GGATC 2 cut(s) 345, 406
Ama87I CYCGRG 4 cut(s) 429, 486, 500, 804
ApeKI GCWGC 1 cut(s) 417
ApoI RAATTY 2 cut(s) 233, 1024
Asp700I GAANNNNTTC 1 cut(s) 438
AspS9I GGNCC 5 cut(s) 155, 320, 453, 461, 952
AsuC2I CCSGG 7 cut(s) 487, 488, 501, 502, 572, 869, 1093
AsuHPI GGTGA 2 cut(s) 337, 395
AvaI CYCGRG 4 cut(s) 429, 486, 500, 804
AvaII GGWCC 1 cut(s) 320
BaeGI GKGCMC 1 cut(s) 762
BanII GRGCYC 2 cut(s) 473, 495
BbsI GAAGAC 2 cut(s) 214, 597
Bbv12I GWGCWC 1 cut(s) 876
BbvCI CCTCAGC 1 cut(s) 74
BbvI GCAGC 1 cut(s) 429
BccI CCATC 2 cut(s) 720, 821
BceAI ACGGC 3 cut(s) 4, 768, 1096
BciT130I CCWGG 2 cut(s) 770, 1001
BcnI CCSGG 7 cut(s) 487, 488, 501, 502, 572, 869, 1093
BcoDI GTCTC 1 cut(s) 63
BfaI CTAG 3 cut(s) 87, 239, 1074
BfmI CTRYAG 1 cut(s) 192
BfuAI ACCTGC 2 cut(s) 397, 420
BisI GCNGC 7 cut(s) 112, 138, 158, 418, 567, 594, 951
BlsI GCNGC 7 cut(s) 113, 139, 159, 419, 568, 595, 952
Bme1390I CCNGG 9 cut(s) 487, 488, 501, 502, 572, 770, 869, 1001, 1093
Bme18I GGWCC 1 cut(s) 320
BmeRI GACNNNNNGTC 1 cut(s) 1001
BmeT110I CYCGRG 4 cut(s) 429, 486, 500, 804
BmgBI CACGTC 1 cut(s) 447
BmgT120I GGNCC 5 cut(s) 155, 320, 453, 461, 952
BmiI GGNNCC 3 cut(s) 454, 492, 541
BmrFI CCNGG 9 cut(s) 487, 488, 501, 502, 572, 770, 869, 1001, 1093
BmsI GCATC 1 cut(s) 662
BpiI GAAGAC 2 cut(s) 214, 597
Bpu10I CCTNAGC 1 cut(s) 74
BpuEI CTTGAG 1 cut(s) 220
BpuMI CCSGG 7 cut(s) 487, 488, 501, 502, 572, 869, 1093
Bsa29I ATCGAT 1 cut(s) 656
BsaHI GRCGYC 2 cut(s) 596, 774
BsaI GGTCTC 1 cut(s) 63
BsaWI WCCGGW 1 cut(s) 322
BsaXI ACNNNNNCTCC 2 cut(s) 349, 379
Bse118I RCCGGY 2 cut(s) 455, 518
Bse3DI GCAATG 1 cut(s) 206
BseBI CCWGG 2 cut(s) 770, 1001
BseCI ATCGAT 1 cut(s) 656
BseGI GGATG 1 cut(s) 102
BseMI GCAATG 1 cut(s) 206
BseMII CTCAG 1 cut(s) 88
BsePI GCGCGC 4 cut(s) 164, 556, 583, 585
BseRI GAGGAG 1 cut(s) 954
BseSI GKGCMC 1 cut(s) 762
BseX3I CGGCCG 1 cut(s) 978
BseXI GCAGC 1 cut(s) 429
BseYI CCCAGC 1 cut(s) 1102
Bsh1285I CGRYCG 1 cut(s) 981
BshVI ATCGAT 1 cut(s) 656
BsiEI CGRYCG 1 cut(s) 981
BsiHKAI GWGCWC 1 cut(s) 876
BsiHKCI CYCGRG 4 cut(s) 429, 486, 500, 804
BsiSI CCGG 8 cut(s) 323, 456, 487, 501, 519, 572, 868, 1092
BslFI GGGAC 3 cut(s) 142, 259, 462
BsmAI GTCTC 1 cut(s) 63
BsmFI GGGAC 3 cut(s) 142, 259, 462
Bso31I GGTCTC 1 cut(s) 63
BsoBI CYCGRG 4 cut(s) 429, 486, 500, 804
Bsp1286I GDGCHC 4 cut(s) 473, 495, 762, 876
Bsp1407I TGTACA 1 cut(s) 286
Bsp143I GATC 3 cut(s) 203, 337, 398
BspCNI CTCAG 1 cut(s) 87
BspDI ATCGAT 1 cut(s) 656
BspLI GGNNCC 3 cut(s) 454, 492, 541
BspMI ACCTGC 2 cut(s) 397, 420
BspPI GGATC 2 cut(s) 345, 406
BspTNI GGTCTC 1 cut(s) 63
BsrBI CCGCTC 2 cut(s) 497, 950
BsrDI GCAATG 1 cut(s) 206
BsrFI RCCGGY 2 cut(s) 455, 518
BsrGI TGTACA 1 cut(s) 286
BssAI RCCGGY 2 cut(s) 455, 518
BssHII GCGCGC 4 cut(s) 164, 556, 583, 585
BssMI GATC 3 cut(s) 203, 337, 398
BssNI GRCGYC 2 cut(s) 596, 774
BssT1I CCWWGG 1 cut(s) 535
Bst2UI CCWGG 2 cut(s) 770, 1001
Bst4CI ACNGT 1 cut(s) 580
BstACI GRCGYC 2 cut(s) 596, 774
BstAUI TGTACA 1 cut(s) 286
BstDEI CTNAG 4 cut(s) 25, 74, 122, 143
BstDSI CCRYGG 1 cut(s) 109
BstEII GGTNACC 1 cut(s) 1013
BstENI CCTNNNNNAGG 1 cut(s) 393
BstF5I GGATG 1 cut(s) 102
BstKTI GATC 3 cut(s) 206, 340, 401
BstMAI GTCTC 1 cut(s) 63
BstMBI GATC 3 cut(s) 203, 337, 398
BstMCI CGRYCG 1 cut(s) 981
BstNI CCWGG 2 cut(s) 770, 1001
BstPI GGTNACC 1 cut(s) 1013
BstSCI CCNGG 9 cut(s) 485, 486, 499, 500, 570, 768, 867, 999, 1091
BstSFI CTRYAG 1 cut(s) 192
BstSLI GKGCMC 1 cut(s) 762
BstV1I GCAGC 1 cut(s) 429
BstV2I GAAGAC 2 cut(s) 214, 597
BstZI CGGCCG 1 cut(s) 978
Bsu15I ATCGAT 1 cut(s) 656
BsuTUI ATCGAT 1 cut(s) 656
BtgI CCRYGG 1 cut(s) 109
BtrI CACGTC 1 cut(s) 447
BtsCI GGATG 1 cut(s) 102
BtsIMutI CAGTG 1 cut(s) 171
BveI ACCTGC 2 cut(s) 397, 420
Cfr10I RCCGGY 2 cut(s) 455, 518
Cfr13I GGNCC 5 cut(s) 155, 320, 453, 461, 952
Cfr42I CCGCGG 1 cut(s) 112
Cfr9I CCCGGG 2 cut(s) 486, 500
ClaI ATCGAT 1 cut(s) 656
CseI GACGC 7 cut(s) 464, 513, 585, 763, 800, 829, 968
Csp6I GTAC 2 cut(s) 287, 507
CviAII CATG 1 cut(s) 15
CviQI GTAC 2 cut(s) 287, 507
DdeI CTNAG 4 cut(s) 25, 74, 122, 143
DpnI GATC 3 cut(s) 205, 339, 400
DpnII GATC 3 cut(s) 203, 337, 398
DriI GACNNNNNGTC 1 cut(s) 1001
EaeI YGGCCR 4 cut(s) 17, 112, 135, 978
EagI CGGCCG 1 cut(s) 978
Eam1105I GACNNNNNGTC 1 cut(s) 1001
EciI GGCGGA 1 cut(s) 836
EclXI CGGCCG 1 cut(s) 978
Eco130I CCWWGG 1 cut(s) 535
Eco24I GRGCYC 2 cut(s) 473, 495
Eco31I GGTCTC 1 cut(s) 63
Eco32I GATATC 1 cut(s) 640
Eco47I GGWCC 1 cut(s) 320
Eco52I CGGCCG 1 cut(s) 978
Eco88I CYCGRG 4 cut(s) 429, 486, 500, 804
Eco91I GGTNACC 1 cut(s) 1013
EcoNI CCTNNNNNAGG 1 cut(s) 393
EcoO65I GGTNACC 1 cut(s) 1013
EcoRI GAATTC 1 cut(s) 233
EcoRII CCWGG 2 cut(s) 768, 999
EcoRV GATATC 1 cut(s) 640
EcoT14I CCWWGG 1 cut(s) 535
EcoT38I GRGCYC 2 cut(s) 473, 495
ErhI CCWWGG 1 cut(s) 535
FaeI CATG 1 cut(s) 18
FaiI YATR 5 cut(s) 16, 93, 134, 194, 1034
FaqI GGGAC 3 cut(s) 142, 259, 462
FatI CATG 1 cut(s) 14
FauI CCCGC 9 cut(s) 452, 502, 571, 582, 801, 855, 962, 1002, 1026
FblI GTMKAC 1 cut(s) 843
Fnu4HI GCNGC 7 cut(s) 112, 138, 158, 418, 567, 594, 951
FokI GGATG 1 cut(s) 89
FriOI GRGCYC 2 cut(s) 473, 495
Fsp4HI GCNGC 7 cut(s) 112, 138, 158, 418, 567, 594, 951
FspBI CTAG 3 cut(s) 87, 239, 1074
FspI TGCGCA 1 cut(s) 416
GluI GCNGC 7 cut(s) 112, 138, 158, 418, 567, 594, 951
GsaI CCCAGC 1 cut(s) 1106
HapII CCGG 8 cut(s) 323, 456, 487, 501, 519, 572, 868, 1092
HgaI GACGC 7 cut(s) 464, 513, 585, 763, 800, 829, 968
Hin1I GRCGYC 2 cut(s) 596, 774
Hin1II CATG 1 cut(s) 18
HincII GTYRAC 3 cut(s) 61, 267, 844
HindII GTYRAC 3 cut(s) 61, 267, 844
HinfI GANTC 6 cut(s) 188, 250, 624, 700, 717, 1067
HpaI GTTAAC 1 cut(s) 61
HpaII CCGG 8 cut(s) 323, 456, 487, 501, 519, 572, 868, 1092
HphI GGTGA 2 cut(s) 337, 395
Hpy166II GTNNAC 6 cut(s) 61, 267, 289, 386, 709, 844
Hpy188I TCNGA 2 cut(s) 337, 947
Hpy188III TCNNGA 1 cut(s) 806
Hpy8I GTNNAC 6 cut(s) 61, 267, 289, 386, 709, 844
Hpy99I CGWCG 9 cut(s) 303, 451, 601, 604, 816, 845, 884, 941, 962
HpyAV CCTTC 3 cut(s) 348, 389, 474
HpyCH4III ACNGT 1 cut(s) 580
HpyCH4IV ACGT 3 cut(s) 272, 446, 666
HpyCH4V TGCA 3 cut(s) 14, 211, 697
HpyF3I CTNAG 4 cut(s) 25, 74, 122, 143
HpySE526I ACGT 3 cut(s) 272, 446, 666
Hsp92I GRCGYC 2 cut(s) 596, 774
Hsp92II CATG 1 cut(s) 18
KroI GCCGGC 1 cut(s) 455
KroNI GCCGGC 1 cut(s) 457
KspAI GTTAAC 1 cut(s) 61
KspI CCGCGG 1 cut(s) 112
Kzo9I GATC 3 cut(s) 203, 337, 398
LmnI GCTCC 8 cut(s) 35, 308, 490, 502, 565, 808, 948, 967
Lsp1109I GCAGC 1 cut(s) 429
LweI GCATC 1 cut(s) 662
MaeI CTAG 3 cut(s) 87, 239, 1074
MaeII ACGT 3 cut(s) 272, 446, 666
MaeIII GTNAC 4 cut(s) 365, 775, 1013, 1076
MalI GATC 3 cut(s) 205, 339, 400
MauBI CGCGCGCG 1 cut(s) 585
MbiI CCGCTC 2 cut(s) 497, 950
MboI GATC 3 cut(s) 203, 337, 398
MboII GAAGA 3 cut(s) 214, 597, 673
MfeI CAATTG 1 cut(s) 212
MhlI GDGCHC 4 cut(s) 473, 495, 762, 876
MluCI AATT 5 cut(s) 52, 212, 233, 692, 1024
MlyI GAGTC 4 cut(s) 197, 259, 618, 726
MmeI TCCRAC 1 cut(s) 933
MroNI GCCGGC 1 cut(s) 455
MroXI GAANNNNTTC 1 cut(s) 438
MseI TTAA 3 cut(s) 51, 60, 1028
MspA1I CMGCKG 2 cut(s) 111, 1021
MspI CCGG 8 cut(s) 323, 456, 487, 501, 519, 572, 868, 1092
MspR9I CCNGG 9 cut(s) 487, 488, 501, 502, 572, 770, 869, 1001, 1093
MunI CAATTG 1 cut(s) 212
MvaI CCWGG 2 cut(s) 770, 1001
NaeI GCCGGC 1 cut(s) 457
NciI CCSGG 7 cut(s) 487, 488, 501, 502, 572, 869, 1093
NdeII GATC 3 cut(s) 203, 337, 398
NgoMIV GCCGGC 1 cut(s) 455
NlaIII CATG 1 cut(s) 18
NlaIV GGNNCC 3 cut(s) 454, 492, 541
NmeAIII GCCGAG 6 cut(s) 608, 623, 779, 855, 950, 1006
NmuCI GTSAC 1 cut(s) 775
NsbI TGCGCA 1 cut(s) 416
PauI GCGCGC 4 cut(s) 164, 556, 583, 585
PcsI WCGNNNNNNNCGW 2 cut(s) 307, 888
PdiI GCCGGC 1 cut(s) 457
PdmI GAANNNNTTC 1 cut(s) 438
PfeI GAWTC 2 cut(s) 700, 1067
PflFI GACNNNGTC 1 cut(s) 271
PkrI GCNGC 7 cut(s) 113, 139, 159, 419, 568, 595, 952
PleI GAGTC 4 cut(s) 196, 258, 618, 725
PpsI GAGTC 4 cut(s) 196, 258, 618, 725
Psp6I CCWGG 2 cut(s) 768, 999
PspEI GGTNACC 1 cut(s) 1013
PspFI CCCAGC 1 cut(s) 1102
PspGI CCWGG 2 cut(s) 768, 999
PspN4I GGNNCC 3 cut(s) 454, 492, 541
PspPI GGNCC 5 cut(s) 155, 320, 453, 461, 952
PsyI GACNNNGTC 1 cut(s) 271
PteI GCGCGC 4 cut(s) 164, 556, 583, 585
RsaI GTAC 2 cut(s) 288, 508
RsaNI GTAC 2 cut(s) 287, 507
SacII CCGCGG 1 cut(s) 112
SalI GTCGAC 1 cut(s) 842
SaqAI TTAA 3 cut(s) 51, 60, 1028
SatI GCNGC 7 cut(s) 112, 138, 158, 418, 567, 594, 951
Sau3AI GATC 3 cut(s) 203, 337, 398
Sau96I GGNCC 5 cut(s) 155, 320, 453, 461, 952
SchI GAGTC 4 cut(s) 197, 259, 618, 726
ScrFI CCNGG 9 cut(s) 487, 488, 501, 502, 572, 770, 869, 1001, 1093
SduI GDGCHC 4 cut(s) 473, 495, 762, 876
SfaNI GCATC 1 cut(s) 662
SfcI CTRYAG 1 cut(s) 192
Sfr303I CCGCGG 1 cut(s) 112
SgrAI CRCCGGYG 1 cut(s) 518
SgrBI CCGCGG 1 cut(s) 112
SinI GGWCC 1 cut(s) 320
SmaI CCCGGG 2 cut(s) 488, 502
SmlI CTYRAG 1 cut(s) 199
SmoI CTYRAG 1 cut(s) 199
Sse9I AATT 5 cut(s) 52, 212, 233, 692, 1024
SspMI CTAG 3 cut(s) 87, 239, 1074
StyD4I CCNGG 9 cut(s) 485, 486, 499, 500, 570, 768, 867, 999, 1091
StyI CCWWGG 1 cut(s) 535
TaaI ACNGT 1 cut(s) 580
TaiI ACGT 3 cut(s) 275, 449, 669
TaqI TCGA 4 cut(s) 407, 656, 715, 843
TasI AATT 5 cut(s) 52, 212, 233, 692, 1024
TatI WGTACW 1 cut(s) 286
TauI GCSGC 6 cut(s) 114, 140, 160, 569, 596, 953
TfiI GAWTC 2 cut(s) 700, 1067
Tru1I TTAA 3 cut(s) 51, 60, 1028
Tru9I TTAA 3 cut(s) 51, 60, 1028
TscAI CASTG 1 cut(s) 178
TseFI GTSAC 1 cut(s) 775
TseI GCWGC 1 cut(s) 417
Tsp45I GTSAC 1 cut(s) 775
TspDTI ATGAA 1 cut(s) 674
TspGWI ACGGA 3 cut(s) 46, 367, 650
TspMI CCCGGG 2 cut(s) 486, 500
TspRI CASTG 1 cut(s) 178
Tth111I GACNNNGTC 1 cut(s) 271
VpaK11BI GGWCC 1 cut(s) 320
XagI CCTNNNNNAGG 1 cut(s) 393
XapI RAATTY 2 cut(s) 233, 1024
XmaI CCCGGG 2 cut(s) 486, 500
XmiI GTMKAC 1 cut(s) 843
XmnI GAANNNNTTC 1 cut(s) 438
XspI CTAG 3 cut(s) 87, 239, 1074
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.