pycom12555g00200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012555
OG0002208
Physical Location & Seq
Reverse (-)
110340 .. 111578
1239 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12555g00200.2

Sequence Viewer

Length: 810 bp
ATGACTCGTAGTTGGACCTTGGGTTGGGTCGACCGGTCCGCCTCTGGTGTGCACCGGTCGGCTCGTCCCTTCTGCCGGCGATACGCTCCTGGCCTTAATTGGCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGAATACATTAGCATGGGATAACATCATAGGATTTCGACGAACAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGGCGGATGTTACTTTTAGGACTCCGCCGGCACCTTATGAGAAATCAAAGTTTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGGTCATCCTCCGCGGCCAGCTTCTTAGAGGGACTATGGCCATCATTGCAATTGTTGGTCTTCAACGAGGAATTCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTCCTACCGATTGAATGGTCCGGTGAAGTGTTCGGATCGCGGCGACGCGGGTGGTTCGCTGCCCGCGACGTCGCGAGAAGTCCACTGAACCTTATCATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

270

Amino Acids

29.37

Weight (kDa)

10.51

Isoelectric Point (pI)

53.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 780
AccB1I GGYRCC 2 cut(s) 352, 433
AccI GTMKAC 1 cut(s) 30
AccII CGCG 7 cut(s) 582, 654, 669, 748, 756, 774, 782
AclWI GGATC 2 cut(s) 328, 751
AcoI YGGCCR 4 cut(s) 101, 488, 583, 606
AcsI RAATTY 1 cut(s) 639
AcyI GRCGYC 1 cut(s) 777
AfaI GTAC 1 cut(s) 694
AfiI CCNNNNNNNGG 3 cut(s) 24, 75, 322
AgeI ACCGGT 2 cut(s) 33, 54
AgsI TTSAA 3 cut(s) 133, 632, 722
AjnI CCWGG 3 cut(s) 88, 315, 439
AjuI GAANNNNNNNTTGG 2 cut(s) 221, 253
AluBI AGCT 3 cut(s) 561, 589, 665
AluI AGCT 3 cut(s) 561, 589, 665
Alw21I GWGCWC 2 cut(s) 54, 149
Alw26I GTCTC 2 cut(s) 298, 534
Alw44I GTGCAC 1 cut(s) 50
AlwI GGATC 2 cut(s) 328, 751
AoxI GGCC 5 cut(s) 91, 101, 488, 583, 606
ApaLI GTGCAC 1 cut(s) 50
ApeKI GCWGC 1 cut(s) 767
ApoI RAATTY 1 cut(s) 639
AseI ATTAAT 1 cut(s) 243
AsiGI ACCGGT 2 cut(s) 33, 54
AspLEI GCGC 3 cut(s) 122, 457, 654
AspS9I GGNCC 3 cut(s) 15, 36, 726
AsuC2I CCSGG 2 cut(s) 105, 385
AsuHPI GGTGA 1 cut(s) 743
AvaII GGWCC 3 cut(s) 15, 36, 726
BaeGI GKGCMC 2 cut(s) 54, 436
BalI TGGCCA 1 cut(s) 608
BanI GGYRCC 2 cut(s) 352, 433
BanII GRGCYC 1 cut(s) 268
BbsI GAAGAC 2 cut(s) 277, 620
Bbv12I GWGCWC 2 cut(s) 54, 149
BbvCI CCTCAGC 1 cut(s) 545
BbvI GCAGC 1 cut(s) 754
BccI CCATC 1 cut(s) 617
BceAI ACGGC 1 cut(s) 475
BciT130I CCWGG 3 cut(s) 90, 317, 441
BcnI CCSGG 2 cut(s) 105, 385
BcoDI GTCTC 2 cut(s) 298, 534
BfaI CTAG 3 cut(s) 290, 558, 645
BfoI RGCGCY 1 cut(s) 123
BisI GCNGC 3 cut(s) 583, 749, 768
BlsI GCNGC 3 cut(s) 584, 750, 769
Bme1390I CCNGG 5 cut(s) 90, 105, 317, 385, 441
Bme18I GGWCC 3 cut(s) 15, 36, 726
BmgT120I GGNCC 3 cut(s) 15, 36, 726
BmiI GGNNCC 4 cut(s) 354, 382, 435, 450
BmrFI CCNGG 5 cut(s) 90, 105, 317, 385, 441
BmsI GCATC 1 cut(s) 298
BpiI GAAGAC 2 cut(s) 277, 620
Bpu10I CCTNAGC 1 cut(s) 545
BpuMI CCSGG 2 cut(s) 105, 385
BsaHI GRCGYC 1 cut(s) 777
BsaI GGTCTC 1 cut(s) 534
BsaJI CCNNGG 5 cut(s) 18, 228, 316, 384, 580
BsaWI WCCGGW 3 cut(s) 33, 54, 728
Bsc4I CCNNNNNNNGG 3 cut(s) 24, 75, 322
Bse118I RCCGGY 4 cut(s) 33, 54, 75, 349
Bse3DI GCAATG 1 cut(s) 612
BseBI CCWGG 3 cut(s) 90, 317, 441
BseDI CCNNGG 5 cut(s) 18, 228, 316, 384, 580
BseGI GGATG 3 cut(s) 238, 334, 573
BseLI CCNNNNNNNGG 3 cut(s) 24, 75, 322
BseMI GCAATG 1 cut(s) 612
BseMII CTCAG 1 cut(s) 559
BseSI GKGCMC 2 cut(s) 54, 436
BseXI GCAGC 1 cut(s) 754
Bsh1236I CGCG 7 cut(s) 582, 654, 669, 748, 756, 774, 782
Bsh1285I CGRYCG 2 cut(s) 34, 59
BshFI GGCC 5 cut(s) 93, 103, 490, 585, 608
BshNI GGYRCC 2 cut(s) 352, 433
BshTI ACCGGT 2 cut(s) 33, 54
BsiEI CGRYCG 2 cut(s) 34, 59
BsiHKAI GWGCWC 2 cut(s) 54, 149
BsiSI CCGG 8 cut(s) 34, 55, 76, 104, 117, 350, 384, 729
BslFI GGGAC 3 cut(s) 51, 613, 665
BslI CCNNNNNNNGG 3 cut(s) 24, 75, 322
BsmAI GTCTC 2 cut(s) 298, 534
BsmFI GGGAC 3 cut(s) 51, 613, 665
BsnI GGCC 5 cut(s) 93, 103, 490, 585, 608
Bso31I GGTCTC 1 cut(s) 534
Bsp1286I GDGCHC 4 cut(s) 54, 149, 268, 436
Bsp1407I TGTACA 1 cut(s) 692
Bsp143I GATC 3 cut(s) 275, 320, 743
Bsp68I TCGCGA 1 cut(s) 782
BspANI GGCC 5 cut(s) 93, 103, 490, 585, 608
BspCNI CTCAG 1 cut(s) 558
BspFNI CGCG 7 cut(s) 582, 654, 669, 748, 756, 774, 782
BspLI GGNNCC 4 cut(s) 354, 382, 435, 450
BspPI GGATC 2 cut(s) 328, 751
BspT107I GGYRCC 2 cut(s) 352, 433
BspTNI GGTCTC 1 cut(s) 534
BsrDI GCAATG 1 cut(s) 612
BsrFI RCCGGY 4 cut(s) 33, 54, 75, 349
BsrGI TGTACA 1 cut(s) 692
BssAI RCCGGY 4 cut(s) 33, 54, 75, 349
BssECI CCNNGG 5 cut(s) 18, 228, 316, 384, 580
BssMI GATC 3 cut(s) 275, 320, 743
BssNI GRCGYC 1 cut(s) 777
BssT1I CCWWGG 2 cut(s) 18, 228
Bst2UI CCWGG 3 cut(s) 90, 317, 441
Bst4CI ACNGT 1 cut(s) 286
BstACI GRCGYC 1 cut(s) 777
BstAUI TGTACA 1 cut(s) 692
BstC8I GCNNGC 8 cut(s) 77, 156, 351, 453, 551, 587, 667, 772
BstDEI CTNAG 3 cut(s) 496, 545, 593
BstDSI CCRYGG 1 cut(s) 580
BstF5I GGATG 3 cut(s) 238, 334, 573
BstFNI CGCG 7 cut(s) 582, 654, 669, 748, 756, 774, 782
BstH2I RGCGCY 1 cut(s) 123
BstHHI GCGC 3 cut(s) 122, 457, 654
BstKTI GATC 3 cut(s) 278, 323, 746
BstMAI GTCTC 2 cut(s) 298, 534
BstMBI GATC 3 cut(s) 275, 320, 743
BstMCI CGRYCG 2 cut(s) 34, 59
BstMWI GCNNNNNNNGC 2 cut(s) 119, 614
BstNI CCWGG 3 cut(s) 90, 317, 441
BstSCI CCNGG 5 cut(s) 88, 103, 315, 383, 439
BstSLI GKGCMC 2 cut(s) 54, 436
BstUI CGCG 7 cut(s) 582, 654, 669, 748, 756, 774, 782
BstV1I GCAGC 1 cut(s) 754
BstV2I GAAGAC 2 cut(s) 277, 620
BsuRI GGCC 5 cut(s) 93, 103, 490, 585, 608
BtgI CCRYGG 1 cut(s) 580
BtsCI GGATG 3 cut(s) 238, 334, 573
BtsIMutI CAGTG 1 cut(s) 791
BtuMI TCGCGA 1 cut(s) 782
Cac8I GCNNGC 8 cut(s) 77, 156, 351, 453, 551, 587, 667, 772
CfoI GCGC 3 cut(s) 122, 457, 654
Cfr10I RCCGGY 4 cut(s) 33, 54, 75, 349
Cfr13I GGNCC 3 cut(s) 15, 36, 726
Cfr42I CCGCGG 1 cut(s) 583
CpoI CGGWCCG 1 cut(s) 36
CseI GACGC 1 cut(s) 762
Csp6I GTAC 1 cut(s) 693
CspAI ACCGGT 2 cut(s) 33, 54
CspI CGGWCCG 1 cut(s) 36
CviAII CATG 2 cut(s) 180, 486
CviQI GTAC 1 cut(s) 693
DdeI CTNAG 3 cut(s) 496, 545, 593
DpnI GATC 3 cut(s) 277, 322, 745
DpnII GATC 3 cut(s) 275, 320, 743
EaeI YGGCCR 4 cut(s) 101, 488, 583, 606
EciI GGCGGA 3 cut(s) 28, 336, 341
Eco130I CCWWGG 2 cut(s) 18, 228
Eco24I GRGCYC 1 cut(s) 268
Eco31I GGTCTC 1 cut(s) 534
Eco47I GGWCC 3 cut(s) 15, 36, 726
EcoRI GAATTC 1 cut(s) 639
EcoRII CCWGG 3 cut(s) 88, 315, 439
EcoT14I CCWWGG 2 cut(s) 18, 228
EcoT38I GRGCYC 1 cut(s) 268
ErhI CCWWGG 2 cut(s) 18, 228
FaeI CATG 2 cut(s) 183, 489
FaiI YATR 9 cut(s) 181, 194, 302, 360, 395, 473, 487, 564, 605
FaqI GGGAC 3 cut(s) 51, 613, 665
FatI CATG 2 cut(s) 179, 485
FauI CCCGC 2 cut(s) 749, 779
FblI GTMKAC 1 cut(s) 30
Fnu4HI GCNGC 3 cut(s) 583, 749, 768
FokI GGATG 3 cut(s) 245, 341, 560
FriOI GRGCYC 1 cut(s) 268
Fsp4HI GCNGC 3 cut(s) 583, 749, 768
FspBI CTAG 3 cut(s) 290, 558, 645
GlaI GCGC 3 cut(s) 121, 456, 653
GluI GCNGC 3 cut(s) 583, 749, 768
HaeII RGCGCY 1 cut(s) 123
HaeIII GGCC 5 cut(s) 93, 103, 490, 585, 608
HapII CCGG 8 cut(s) 34, 55, 76, 104, 117, 350, 384, 729
HgaI GACGC 1 cut(s) 762
HhaI GCGC 3 cut(s) 122, 457, 654
Hin1I GRCGYC 1 cut(s) 777
Hin1II CATG 2 cut(s) 183, 489
Hin6I GCGC 3 cut(s) 120, 455, 652
HinP1I GCGC 3 cut(s) 120, 455, 652
HincII GTYRAC 3 cut(s) 31, 532, 673
HindII GTYRAC 3 cut(s) 31, 532, 673
HinfI GANTC 4 cut(s) 4, 343, 467, 656
HpaI GTTAAC 1 cut(s) 532
HpaII CCGG 8 cut(s) 34, 55, 76, 104, 117, 350, 384, 729
HphI GGTGA 1 cut(s) 743
Hpy166II GTNNAC 6 cut(s) 31, 52, 532, 673, 695, 791
Hpy188I TCNGA 3 cut(s) 168, 280, 743
Hpy188III TCNNGA 3 cut(s) 248, 464, 781
Hpy8I GTNNAC 6 cut(s) 31, 52, 532, 673, 695, 791
Hpy99I CGWCG 5 cut(s) 207, 709, 756, 779, 782
HpyAV CCTTC 2 cut(s) 79, 423
HpyCH4III ACNGT 1 cut(s) 286
HpyCH4IV ACGT 2 cut(s) 678, 777
HpyCH4V TGCA 3 cut(s) 52, 485, 617
HpyF10VI GCNNNNNNNGC 2 cut(s) 119, 614
HpyF3I CTNAG 3 cut(s) 496, 545, 593
HpySE526I ACGT 2 cut(s) 678, 777
Hsp92I GRCGYC 1 cut(s) 777
Hsp92II CATG 2 cut(s) 183, 489
HspAI GCGC 3 cut(s) 120, 455, 652
KroI GCCGGC 2 cut(s) 75, 349
KroNI GCCGGC 2 cut(s) 77, 351
KspAI GTTAAC 1 cut(s) 532
KspI CCGCGG 1 cut(s) 583
Kzo9I GATC 3 cut(s) 275, 320, 743
LmnI GCTCC 4 cut(s) 91, 448, 506, 714
Lsp1109I GCAGC 1 cut(s) 754
LweI GCATC 1 cut(s) 298
MaeI CTAG 3 cut(s) 290, 558, 645
MaeII ACGT 2 cut(s) 678, 777
MaeIII GTNAC 2 cut(s) 124, 331
MalI GATC 3 cut(s) 277, 322, 745
MboI GATC 3 cut(s) 275, 320, 743
MboII GAAGA 3 cut(s) 145, 282, 620
MfeI CAATTG 1 cut(s) 618
MhlI GDGCHC 4 cut(s) 54, 149, 268, 436
MlsI TGGCCA 1 cut(s) 608
MluCI AATT 6 cut(s) 97, 137, 420, 523, 618, 639
MluNI TGGCCA 1 cut(s) 608
MlyI GAGTC 2 cut(s) 337, 665
MnlI CCTC 7 cut(s) 52, 124, 554, 562, 587, 590, 629
Mox20I TGGCCA 1 cut(s) 608
MroNI GCCGGC 2 cut(s) 75, 349
MscI TGGCCA 1 cut(s) 608
MseI TTAA 5 cut(s) 96, 243, 414, 522, 531
MslI CAYNNNNRTG 1 cut(s) 178
Msp20I TGGCCA 1 cut(s) 608
MspA1I CMGCKG 1 cut(s) 582
MspI CCGG 8 cut(s) 34, 55, 76, 104, 117, 350, 384, 729
MspR9I CCNGG 5 cut(s) 90, 105, 317, 385, 441
MunI CAATTG 1 cut(s) 618
MvaI CCWGG 3 cut(s) 90, 317, 441
MvnI CGCG 7 cut(s) 582, 654, 669, 748, 756, 774, 782
MwoI GCNNNNNNNGC 2 cut(s) 119, 614
NaeI GCCGGC 2 cut(s) 77, 351
NciI CCSGG 2 cut(s) 105, 385
NdeII GATC 3 cut(s) 275, 320, 743
NgoMIV GCCGGC 2 cut(s) 75, 349
NlaIII CATG 2 cut(s) 183, 489
NlaIV GGNNCC 4 cut(s) 354, 382, 435, 450
NruI TCGCGA 1 cut(s) 782
PcsI WCGNNNNNNNCGW 2 cut(s) 713, 771
PdiI GCCGGC 2 cut(s) 77, 351
PfeI GAWTC 1 cut(s) 467
PflFI GACNNNGTC 1 cut(s) 677
PinAI ACCGGT 2 cut(s) 33, 54
PkrI GCNGC 3 cut(s) 584, 750, 769
PleI GAGTC 2 cut(s) 337, 664
PpsI GAGTC 2 cut(s) 337, 664
PshBI ATTAAT 1 cut(s) 243
Psp6I CCWGG 3 cut(s) 88, 315, 439
PspGI CCWGG 3 cut(s) 88, 315, 439
PspN4I GGNNCC 4 cut(s) 354, 382, 435, 450
PspPI GGNCC 3 cut(s) 15, 36, 726
PsyI GACNNNGTC 1 cut(s) 677
RruI TCGCGA 1 cut(s) 782
RsaI GTAC 1 cut(s) 694
RsaNI GTAC 1 cut(s) 693
RseI CAYNNNNRTG 1 cut(s) 178
Rsr2I CGGWCCG 1 cut(s) 36
RsrII CGGWCCG 1 cut(s) 36
SacII CCGCGG 1 cut(s) 583
SalI GTCGAC 1 cut(s) 29
SaqAI TTAA 5 cut(s) 96, 243, 414, 522, 531
SatI GCNGC 3 cut(s) 583, 749, 768
Sau3AI GATC 3 cut(s) 275, 320, 743
Sau96I GGNCC 3 cut(s) 15, 36, 726
SchI GAGTC 2 cut(s) 337, 665
ScrFI CCNGG 5 cut(s) 90, 105, 317, 385, 441
SduI GDGCHC 4 cut(s) 54, 149, 268, 436
SfaNI GCATC 1 cut(s) 298
Sfr303I CCGCGG 1 cut(s) 583
SgrBI CCGCGG 1 cut(s) 583
SinI GGWCC 3 cut(s) 15, 36, 726
SmiMI CAYNNNNRTG 1 cut(s) 178
Sse9I AATT 6 cut(s) 97, 137, 420, 523, 618, 639
SspMI CTAG 3 cut(s) 290, 558, 645
StyD4I CCNGG 5 cut(s) 88, 103, 315, 383, 439
StyI CCWWGG 2 cut(s) 18, 228
TaaI ACNGT 1 cut(s) 286
TaiI ACGT 2 cut(s) 681, 780
TaqI TCGA 3 cut(s) 30, 202, 268
TasI AATT 6 cut(s) 97, 137, 420, 523, 618, 639
TatI WGTACW 1 cut(s) 692
TauI GCSGC 2 cut(s) 585, 751
TfiI GAWTC 1 cut(s) 467
Tru1I TTAA 5 cut(s) 96, 243, 414, 522, 531
Tru9I TTAA 5 cut(s) 96, 243, 414, 522, 531
TscAI CASTG 1 cut(s) 798
TseI GCWGC 1 cut(s) 767
TspDTI ATGAA 1 cut(s) 229
TspGWI ACGGA 2 cut(s) 441, 517
TspRI CASTG 1 cut(s) 798
Tth111I GACNNNGTC 1 cut(s) 677
VneI GTGCAC 1 cut(s) 50
VpaK11BI GGWCC 3 cut(s) 15, 36, 726
VspI ATTAAT 1 cut(s) 243
XapI RAATTY 1 cut(s) 639
XmiI GTMKAC 1 cut(s) 30
XspI CTAG 3 cut(s) 290, 558, 645
ZraI GACGTC 1 cut(s) 778
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.