pycom2246g00010

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00002246
OG0002208
Physical Location & Seq
Forward (+)
27 .. 1740
1714 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom2246g00010.3

Sequence Viewer

Length: 813 bp
ATGCCAAGCCTCGCAAAATGTCGACCATGGCATGCCAATGACATGCCAAGCCTCGCAAAATGTCGACCGTGGCATGCCAATGACATGCCAATGTCATGCCAAGCCTCGCAAAATGTCGACCGTGGCTTGCCAATGTCATGCCAAGGGTCGCAAAATGTCGGCCATGGCATGCCAATGACATGCCAAGCCTCGCAAAATGTCGACCGTGGCATGCCAATGACATGCCAACCTCGCATCTTGGCACTCAAAAAACTCGCTGCTAGCCTCGCCACTAGCCTCGCTTCCTGTCGACAGCAAGCGAGGCTAGCGCCCGCTCTGACATGTCAAATTGCACAGCAAAATATGCTATATAGGGGGGTGGTGCTCCATGCATTTCAAGCAGCCTGCACCATCGCTCTAGGGATGACATTCTCGACCATCATCACGTGTTGCCTCGGTATATATGATGTGTCGGGTTTGGGACATTCCCGCCCATGGTGCCGACGACGGTGGCGGGCACCCCCGCCCCCGCCGCCGCCCCCCCCCCCCATGGGTGGGGGACAAAATGGTTTTAAGCGGGGTGGAAAAAACGTTTTGAAAAAGGGCGGGTTGGAAAAAATATTTTCTCCTAAAAAAATAGCATGGGATTATTTGTTGCAAGCAGGGGGTGGGAAAGATTTTGGGGTGGGATTAAGGGGTTGGTGCTATGTGTTAGGTCCTTCCCAACCCCCGTTGCATGTCGAAAGCGAGTTCGTGCGGCGTCGACGAAGCTTTTCGGTAGCCGTTGAACTGTTCGGTTGTCCCGGTCGCCTTCGTACGTGTTTCCATGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

271

Amino Acids

29.46

Weight (kDa)

9.93

Isoelectric Point (pI)

66.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 477, 496
AccBSI CCGCTC 1 cut(s) 314
AccI GTMKAC 6 cut(s) 22, 64, 117, 201, 289, 742
AclI AACGTT 1 cut(s) 570
AcoI YGGCCR 1 cut(s) 160
AcvI CACGTG 1 cut(s) 426
AcyI GRCGYC 1 cut(s) 739
AfaI GTAC 1 cut(s) 796
AfiI CCNNNNNNNGG 5 cut(s) 474, 529, 530, 533, 534
AflIII ACRYGT 3 cut(s) 320, 425, 797
AgsI TTSAA 3 cut(s) 377, 577, 767
AluBI AGCT 1 cut(s) 750
AluI AGCT 1 cut(s) 750
Alw21I GWGCWC 1 cut(s) 366
AoxI GGCC 1 cut(s) 160
ApeKI GCWGC 2 cut(s) 257, 380
Asp700I GAANNNNTTC 1 cut(s) 751
AspLEI GCGC 1 cut(s) 310
AspS9I GGNCC 1 cut(s) 695
AsuC2I CCSGG 1 cut(s) 783
AsuNHI GCTAGC 2 cut(s) 260, 304
AvaII GGWCC 1 cut(s) 695
BaeGI GKGCMC 1 cut(s) 499
BanI GGYRCC 2 cut(s) 477, 496
BbrPI CACGTG 1 cut(s) 426
Bbv12I GWGCWC 1 cut(s) 366
BbvI GCAGC 2 cut(s) 244, 392
BccI CCATC 2 cut(s) 398, 425
BceAI ACGGC 1 cut(s) 746
BcnI CCSGG 1 cut(s) 783
BfaI CTAG 5 cut(s) 261, 273, 305, 398, 811
BfoI RGCGCY 1 cut(s) 311
BisI GCNGC 5 cut(s) 258, 381, 512, 515, 737
BlsI GCNGC 5 cut(s) 259, 382, 513, 516, 738
Bme1390I CCNGG 1 cut(s) 783
Bme18I GGWCC 1 cut(s) 695
BmgT120I GGNCC 1 cut(s) 695
BmiI GGNNCC 2 cut(s) 479, 498
BmrFI CCNGG 1 cut(s) 783
BmsI GCATC 1 cut(s) 243
BmtI GCTAGC 2 cut(s) 264, 308
BpuMI CCSGG 1 cut(s) 783
BsaAI YACGTR 2 cut(s) 426, 798
BsaHI GRCGYC 1 cut(s) 739
BsaJI CCNNGG 9 cut(s) 26, 68, 121, 142, 163, 205, 433, 473, 528
Bsc4I CCNNNNNNNGG 5 cut(s) 474, 529, 530, 533, 534
BseDI CCNNGG 9 cut(s) 26, 68, 121, 142, 163, 205, 433, 473, 528
BseGI GGATG 1 cut(s) 408
BseLI CCNNNNNNNGG 5 cut(s) 474, 529, 530, 533, 534
BseSI GKGCMC 1 cut(s) 499
BseXI GCAGC 2 cut(s) 244, 392
BsgI GTGCAG 1 cut(s) 370
Bsh1285I CGRYCG 4 cut(s) 68, 121, 205, 787
BshFI GGCC 1 cut(s) 162
BshNI GGYRCC 2 cut(s) 477, 496
BsiEI CGRYCG 4 cut(s) 68, 121, 205, 787
BsiHKAI GWGCWC 1 cut(s) 366
BsiSI CCGG 1 cut(s) 783
BsiWI CGTACG 1 cut(s) 794
BslFI GGGAC 3 cut(s) 474, 552, 765
BslI CCNNNNNNNGG 5 cut(s) 474, 529, 530, 533, 534
BsmFI GGGAC 3 cut(s) 474, 552, 765
BsnI GGCC 1 cut(s) 162
Bsp1286I GDGCHC 2 cut(s) 366, 499
Bsp19I CCATGG 4 cut(s) 26, 163, 473, 528
BspANI GGCC 1 cut(s) 162
BspLI GGNNCC 2 cut(s) 479, 498
BspOI GCTAGC 2 cut(s) 264, 308
BspT107I GGYRCC 2 cut(s) 477, 496
BsrBI CCGCTC 1 cut(s) 314
BssECI CCNNGG 9 cut(s) 26, 68, 121, 142, 163, 205, 433, 473, 528
BssNI GRCGYC 1 cut(s) 739
BssT1I CCWWGG 5 cut(s) 26, 142, 163, 473, 528
Bst4CI ACNGT 5 cut(s) 69, 122, 206, 489, 771
BstACI GRCGYC 1 cut(s) 739
BstAPI GCANNNNNTGC 1 cut(s) 343
BstBAI YACGTR 2 cut(s) 426, 798
BstDSI CCRYGG 7 cut(s) 26, 68, 121, 163, 205, 473, 528
BstF5I GGATG 1 cut(s) 408
BstH2I RGCGCY 1 cut(s) 311
BstHHI GCGC 1 cut(s) 310
BstMCI CGRYCG 4 cut(s) 68, 121, 205, 787
BstMWI GCNNNNNNNGC 8 cut(s) 231, 266, 301, 305, 343, 377, 477, 511
BstSCI CCNGG 1 cut(s) 781
BstSLI GKGCMC 1 cut(s) 499
BstV1I GCAGC 2 cut(s) 244, 392
BsuRI GGCC 1 cut(s) 162
BtgI CCRYGG 7 cut(s) 26, 68, 121, 163, 205, 473, 528
BtgZI GCGATG 1 cut(s) 376
BtsCI GGATG 1 cut(s) 408
CfoI GCGC 1 cut(s) 310
Cfr13I GGNCC 1 cut(s) 695
CseI GACGC 1 cut(s) 728
Csp6I GTAC 1 cut(s) 795
CviQI GTAC 1 cut(s) 795
EaeI YGGCCR 1 cut(s) 160
Eco130I CCWWGG 5 cut(s) 26, 142, 163, 473, 528
Eco47I GGWCC 1 cut(s) 695
Eco72I CACGTG 1 cut(s) 426
EcoO109I RGGNCCY 1 cut(s) 695
EcoT14I CCWWGG 5 cut(s) 26, 142, 163, 473, 528
EcoT22I ATGCAT 1 cut(s) 373
ErhI CCWWGG 5 cut(s) 26, 142, 163, 473, 528
FaqI GGGAC 3 cut(s) 474, 552, 765
FauI CCCGC 7 cut(s) 319, 476, 486, 510, 516, 549, 578
FblI GTMKAC 6 cut(s) 22, 64, 117, 201, 289, 742
Fnu4HI GCNGC 5 cut(s) 258, 381, 512, 515, 737
FokI GGATG 1 cut(s) 415
Fsp4HI GCNGC 5 cut(s) 258, 381, 512, 515, 737
FspBI CTAG 5 cut(s) 261, 273, 305, 398, 811
GlaI GCGC 1 cut(s) 309
GluI GCNGC 5 cut(s) 258, 381, 512, 515, 737
HaeII RGCGCY 1 cut(s) 311
HaeIII GGCC 1 cut(s) 162
HapII CCGG 1 cut(s) 783
HgaI GACGC 1 cut(s) 728
HhaI GCGC 1 cut(s) 310
Hin1I GRCGYC 1 cut(s) 739
Hin6I GCGC 1 cut(s) 308
HinP1I GCGC 1 cut(s) 308
HincII GTYRAC 6 cut(s) 23, 65, 118, 202, 290, 743
HindII GTYRAC 6 cut(s) 23, 65, 118, 202, 290, 743
HindIII AAGCTT 1 cut(s) 748
HpaII CCGG 1 cut(s) 783
Hpy166II GTNNAC 6 cut(s) 23, 65, 118, 202, 290, 743
Hpy188I TCNGA 1 cut(s) 318
Hpy188III TCNNGA 1 cut(s) 412
Hpy8I GTNNAC 6 cut(s) 23, 65, 118, 202, 290, 743
Hpy99I CGWCG 4 cut(s) 486, 489, 744, 747
HpyAV CCTTC 2 cut(s) 708, 800
HpyCH4III ACNGT 5 cut(s) 69, 122, 206, 489, 771
HpyCH4IV ACGT 3 cut(s) 425, 570, 797
HpyCH4V TGCA 5 cut(s) 332, 371, 387, 637, 715
HpyF10VI GCNNNNNNNGC 8 cut(s) 231, 266, 301, 305, 343, 377, 477, 511
HpySE526I ACGT 3 cut(s) 425, 570, 797
Hsp92I GRCGYC 1 cut(s) 739
HspAI GCGC 1 cut(s) 308
LmnI GCTCC 1 cut(s) 369
LpnPI CCDG 4 cut(s) 298, 397, 627, 796
Lsp1109I GCAGC 2 cut(s) 244, 392
LweI GCATC 1 cut(s) 243
MaeI CTAG 5 cut(s) 261, 273, 305, 398, 811
MaeII ACGT 3 cut(s) 425, 570, 797
MbiI CCGCTC 1 cut(s) 314
MhlI GDGCHC 2 cut(s) 366, 499
MluCI AATT 1 cut(s) 327
MmeI TCCRAC 1 cut(s) 570
MnlI CCTC 9 cut(s) 20, 62, 115, 199, 240, 275, 287, 294, 443
Mph1103I ATGCAT 1 cut(s) 373
MroXI GAANNNNTTC 1 cut(s) 751
MseI TTAA 2 cut(s) 552, 671
MslI CAYNNNNRTG 5 cut(s) 36, 78, 89, 173, 215
MspI CCGG 1 cut(s) 783
MspR9I CCNGG 1 cut(s) 783
MwoI GCNNNNNNNGC 8 cut(s) 231, 266, 301, 305, 343, 377, 477, 511
NciI CCSGG 1 cut(s) 783
NcoI CCATGG 4 cut(s) 26, 163, 473, 528
NheI GCTAGC 2 cut(s) 260, 304
NlaIV GGNNCC 2 cut(s) 479, 498
NsiI ATGCAT 1 cut(s) 373
PaeI GCATGC 4 cut(s) 35, 77, 172, 214
PciI ACATGT 1 cut(s) 320
PdmI GAANNNNTTC 1 cut(s) 751
Pfl23II CGTACG 1 cut(s) 794
PkrI GCNGC 5 cut(s) 259, 382, 513, 516, 738
PmaCI CACGTG 1 cut(s) 426
PmlI CACGTG 1 cut(s) 426
Ppu21I YACGTR 2 cut(s) 426, 798
PpuMI RGGWCCY 1 cut(s) 695
PscI ACATGT 1 cut(s) 320
Psp1406I AACGTT 1 cut(s) 570
Psp5II RGGWCCY 1 cut(s) 695
PspCI CACGTG 1 cut(s) 426
PspLI CGTACG 1 cut(s) 794
PspN4I GGNNCC 2 cut(s) 479, 498
PspPI GGNCC 1 cut(s) 695
PspPPI RGGWCCY 1 cut(s) 695
RsaI GTAC 1 cut(s) 796
RsaNI GTAC 1 cut(s) 795
RseI CAYNNNNRTG 5 cut(s) 36, 78, 89, 173, 215
SalI GTCGAC 6 cut(s) 21, 63, 116, 200, 288, 741
SaqAI TTAA 2 cut(s) 552, 671
SatI GCNGC 5 cut(s) 258, 381, 512, 515, 737
Sau96I GGNCC 1 cut(s) 695
ScrFI CCNGG 1 cut(s) 783
SduI GDGCHC 2 cut(s) 366, 499
SetI ASST 6 cut(s) 232, 428, 573, 697, 752, 800
SfaNI GCATC 1 cut(s) 243
SgrDI CGTCGACG 1 cut(s) 741
SinI GGWCC 1 cut(s) 695
SmiMI CAYNNNNRTG 5 cut(s) 36, 78, 89, 173, 215
SphI GCATGC 4 cut(s) 35, 77, 172, 214
Sse9I AATT 1 cut(s) 327
SspI AATATT 1 cut(s) 600
SspMI CTAG 5 cut(s) 261, 273, 305, 398, 811
StyD4I CCNGG 1 cut(s) 781
StyI CCWWGG 5 cut(s) 26, 142, 163, 473, 528
TaaI ACNGT 5 cut(s) 69, 122, 206, 489, 771
TaiI ACGT 3 cut(s) 428, 573, 800
TaqI TCGA 8 cut(s) 22, 64, 117, 201, 289, 413, 720, 742
TasI AATT 1 cut(s) 327
TauI GCSGC 3 cut(s) 514, 517, 739
Tru1I TTAA 2 cut(s) 552, 671
Tru9I TTAA 2 cut(s) 552, 671
TseI GCWGC 2 cut(s) 257, 380
VpaK11BI GGWCC 1 cut(s) 695
XmiI GTMKAC 6 cut(s) 22, 64, 117, 201, 289, 742
XmnI GAANNNNTTC 1 cut(s) 751
XspI CTAG 5 cut(s) 261, 273, 305, 398, 811
Zsp2I ATGCAT 1 cut(s) 373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.