pycom2132g00040

Conserved gene of

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00002132
OG0002208
Physical Location & Seq
Forward (+)
10153 .. 13803
3651 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom2132g00040.1

Sequence Viewer

Length: 498 bp
ATGAAGAACGTAGCGAAATGCGATACTTGGTGTGAATTGCAGAATCCCGTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAAGCCGTTAGGCCGAGGGCACGCCTGCCTGGGCGTCACACGCCGTTGCCCCCCCGCCTCGGGCACGCCTGCCTGGGCGTCACACGCCGTTGCCCCCCCGCGCCTCCCCCGGGAGCGTCGGGGGGCGGACGATGGCCTCCCGTGCGTCACCCTGCGCGGTTGGCACAAATGCCGGGTCCTCGGCGACGAACGCCACGACGATCGGTGGTTTCCCAACCTCGGTTGCATGTTGTGCGCTTTCGTCGCGTTCCGAGCGGCCCGCGACGCTCCCGGCTCTGCTTCGGCCGAGCTTTCAACGCGACCCCAGGTCAGGCGGGGTTACCCGCTGAATTTAAGCATATCAATAAGCGGAGGAAAAGAAACTTACGAGGATTCCCCTAGTAACGGCGAGCGAACCGGGAACAGCCCAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

166

Amino Acids

18.15

Weight (kDa)

9.64

Isoelectric Point (pI)

52.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 338
AccII CGCG 5 cut(s) 184, 240, 329, 345, 382
AciI CCGC 9 cut(s) 138, 182, 209, 240, 338, 343, 397, 407, 432
AcoI YGGCCR 1 cut(s) 366
AcsI RAATTY 1 cut(s) 412
AcyI GRCGYC 2 cut(s) 117, 161
AfiI CCNNNNNNNGG 6 cut(s) 142, 143, 193, 302, 393, 467
AgsI TTSAA 2 cut(s) 68, 378
AhdI GACNNNNNGTC 1 cut(s) 389
AjnI CCWGG 3 cut(s) 111, 155, 387
AluBI AGCT 2 cut(s) 373, 494
AluI AGCT 2 cut(s) 373, 494
Ama87I CYCGRG 2 cut(s) 142, 192
AoxI GGCC 4 cut(s) 94, 217, 339, 366
ApoI RAATTY 1 cut(s) 412
ArsI GACNNNNNNTTYG 2 cut(s) 243, 275
AspLEI GCGC 4 cut(s) 81, 186, 240, 320
AspS9I GGNCC 2 cut(s) 259, 340
AsuC2I CCSGG 5 cut(s) 193, 194, 257, 354, 481
AsuHPI GGTGA 1 cut(s) 223
AvaI CYCGRG 2 cut(s) 142, 192
AvaII GGWCC 1 cut(s) 259
BaeGI GKGCMC 2 cut(s) 105, 149
BccI CCATC 2 cut(s) 63, 209
BceAI ACGGC 4 cut(s) 73, 111, 155, 484
BciT130I CCWGG 3 cut(s) 113, 157, 389
BcnI CCSGG 5 cut(s) 193, 194, 257, 354, 481
BfaI CTAG 1 cut(s) 462
BisI GCNGC 1 cut(s) 339
BlsI GCNGC 1 cut(s) 340
Bme1390I CCNGG 8 cut(s) 113, 157, 193, 194, 257, 354, 389, 481
Bme18I GGWCC 1 cut(s) 259
BmeRI GACNNNNNGTC 1 cut(s) 389
BmeT110I CYCGRG 2 cut(s) 142, 192
BmgT120I GGNCC 2 cut(s) 259, 340
BmiI GGNNCC 1 cut(s) 260
BmrFI CCNGG 8 cut(s) 113, 157, 193, 194, 257, 354, 389, 481
BpuMI CCSGG 5 cut(s) 193, 194, 257, 354, 481
BsaHI GRCGYC 2 cut(s) 117, 161
BsaJI CCNNGG 9 cut(s) 97, 112, 141, 156, 191, 192, 262, 301, 387
Bsc4I CCNNNNNNNGG 6 cut(s) 142, 143, 193, 302, 393, 467
BseBI CCWGG 3 cut(s) 113, 157, 389
BseDI CCNNGG 9 cut(s) 97, 112, 141, 156, 191, 192, 262, 301, 387
BseLI CCNNNNNNNGG 6 cut(s) 142, 143, 193, 302, 393, 467
BseSI GKGCMC 2 cut(s) 105, 149
BseX3I CGGCCG 1 cut(s) 366
BseYI CCCAGC 1 cut(s) 490
Bsh1236I CGCG 5 cut(s) 184, 240, 329, 345, 382
Bsh1285I CGRYCG 2 cut(s) 286, 369
BshFI GGCC 4 cut(s) 96, 219, 341, 368
BsiEI CGRYCG 2 cut(s) 286, 369
BsiHKCI CYCGRG 2 cut(s) 142, 192
BsiSI CCGG 4 cut(s) 193, 256, 354, 480
BslI CCNNNNNNNGG 6 cut(s) 142, 143, 193, 302, 393, 467
BsnI GGCC 4 cut(s) 96, 219, 341, 368
BsoBI CYCGRG 2 cut(s) 142, 192
Bsp1286I GDGCHC 2 cut(s) 105, 149
Bsp143I GATC 1 cut(s) 283
BspACI CCGC 9 cut(s) 138, 182, 209, 240, 338, 343, 397, 407, 432
BspANI GGCC 4 cut(s) 96, 219, 341, 368
BspFNI CGCG 5 cut(s) 184, 240, 329, 345, 382
BspLI GGNNCC 1 cut(s) 260
BsrBI CCGCTC 1 cut(s) 338
BssECI CCNNGG 9 cut(s) 97, 112, 141, 156, 191, 192, 262, 301, 387
BssMI GATC 1 cut(s) 283
BssNI GRCGYC 2 cut(s) 117, 161
Bst2UI CCWGG 3 cut(s) 113, 157, 389
BstACI GRCGYC 2 cut(s) 117, 161
BstAPI GCANNNNNTGC 1 cut(s) 315
BstC8I GCNNGC 6 cut(s) 105, 109, 149, 153, 343, 473
BstEII GGTNACC 1 cut(s) 401
BstFNI CGCG 5 cut(s) 184, 240, 329, 345, 382
BstHHI GCGC 4 cut(s) 81, 186, 240, 320
BstKTI GATC 1 cut(s) 286
BstMBI GATC 1 cut(s) 283
BstMCI CGRYCG 2 cut(s) 286, 369
BstNI CCWGG 3 cut(s) 113, 157, 389
BstNSI RCATGY 1 cut(s) 313
BstPI GGTNACC 1 cut(s) 401
BstSCI CCNGG 8 cut(s) 111, 155, 191, 192, 255, 352, 387, 479
BstSLI GKGCMC 2 cut(s) 105, 149
BstUI CGCG 5 cut(s) 184, 240, 329, 345, 382
BstZI CGGCCG 1 cut(s) 366
BsuRI GGCC 4 cut(s) 96, 219, 341, 368
Cac8I GCNNGC 6 cut(s) 105, 109, 149, 153, 343, 473
CfoI GCGC 4 cut(s) 81, 186, 240, 320
Cfr13I GGNCC 2 cut(s) 259, 340
Cfr9I CCCGGG 1 cut(s) 192
CseI GACGC 5 cut(s) 106, 150, 188, 217, 356
CviAII CATG 1 cut(s) 310
CviJI RGCY 9 cut(s) 88, 96, 219, 341, 357, 368, 373, 489, 494
CviKI_1 RGCY 9 cut(s) 88, 96, 219, 341, 357, 368, 373, 489, 494
DpnI GATC 1 cut(s) 285
DpnII GATC 1 cut(s) 283
DriI GACNNNNNGTC 1 cut(s) 389
EaeI YGGCCR 1 cut(s) 366
EagI CGGCCG 1 cut(s) 366
Eam1105I GACNNNNNGTC 1 cut(s) 389
EciI GGCGGA 1 cut(s) 224
EclXI CGGCCG 1 cut(s) 366
Eco47I GGWCC 1 cut(s) 259
Eco52I CGGCCG 1 cut(s) 366
Eco88I CYCGRG 2 cut(s) 142, 192
Eco91I GGTNACC 1 cut(s) 401
EcoO109I RGGNCCY 1 cut(s) 259
EcoO65I GGTNACC 1 cut(s) 401
EcoRII CCWGG 3 cut(s) 111, 155, 387
FaeI CATG 1 cut(s) 313
FaiI YATR 2 cut(s) 311, 422
FatI CATG 1 cut(s) 309
FauI CCCGC 5 cut(s) 145, 189, 350, 390, 414
Fnu4HI GCNGC 1 cut(s) 339
Fsp4HI GCNGC 1 cut(s) 339
FspBI CTAG 1 cut(s) 462
GlaI GCGC 4 cut(s) 80, 185, 239, 319
GluI GCNGC 1 cut(s) 339
GsaI CCCAGC 1 cut(s) 494
HaeIII GGCC 4 cut(s) 96, 219, 341, 368
HapII CCGG 4 cut(s) 193, 256, 354, 480
HgaI GACGC 5 cut(s) 106, 150, 188, 217, 356
HhaI GCGC 4 cut(s) 81, 186, 240, 320
Hin1I GRCGYC 2 cut(s) 117, 161
Hin1II CATG 1 cut(s) 313
Hin6I GCGC 4 cut(s) 79, 184, 238, 318
HinP1I GCGC 4 cut(s) 79, 184, 238, 318
HinfI GANTC 3 cut(s) 43, 60, 455
HpaII CCGG 4 cut(s) 193, 256, 354, 480
HphI GGTGA 1 cut(s) 223
Hpy166II GTNNAC 1 cut(s) 52
Hpy188I TCNGA 1 cut(s) 335
Hpy8I GTNNAC 1 cut(s) 52
Hpy99I CGWCG 5 cut(s) 204, 272, 284, 329, 350
HpyCH4IV ACGT 1 cut(s) 9
HpyCH4V TGCA 2 cut(s) 40, 309
HpySE526I ACGT 1 cut(s) 9
Hsp92I GRCGYC 2 cut(s) 117, 161
Hsp92II CATG 1 cut(s) 313
HspAI GCGC 4 cut(s) 79, 184, 238, 318
Kzo9I GATC 1 cut(s) 283
LmnI GCTCC 2 cut(s) 196, 355
MaeI CTAG 1 cut(s) 462
MaeII ACGT 1 cut(s) 9
MaeIII GTNAC 5 cut(s) 118, 162, 229, 401, 464
MalI GATC 1 cut(s) 285
MbiI CCGCTC 1 cut(s) 338
MboI GATC 1 cut(s) 283
MboII GAAGA 1 cut(s) 16
MhlI GDGCHC 2 cut(s) 105, 149
MluCI AATT 2 cut(s) 35, 412
MlyI GAGTC 1 cut(s) 69
MnlI CCTC 8 cut(s) 92, 151, 197, 230, 272, 311, 428, 445
MseI TTAA 1 cut(s) 416
MspA1I CMGCKG 1 cut(s) 409
MspI CCGG 4 cut(s) 193, 256, 354, 480
MspR9I CCNGG 8 cut(s) 113, 157, 193, 194, 257, 354, 389, 481
MvaI CCWGG 3 cut(s) 113, 157, 389
MvnI CGCG 5 cut(s) 184, 240, 329, 345, 382
NciI CCSGG 5 cut(s) 193, 194, 257, 354, 481
NdeII GATC 1 cut(s) 283
NlaIII CATG 1 cut(s) 313
NlaIV GGNNCC 1 cut(s) 260
NmeAIII GCCGAG 3 cut(s) 122, 243, 394
NmuCI GTSAC 3 cut(s) 118, 162, 229
NspI RCATGY 1 cut(s) 313
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PfeI GAWTC 2 cut(s) 43, 455
PkrI GCNGC 1 cut(s) 340
Ple19I CGATCG 1 cut(s) 286
PleI GAGTC 1 cut(s) 68
PpsI GAGTC 1 cut(s) 68
PpuMI RGGWCCY 1 cut(s) 259
Psp5II RGGWCCY 1 cut(s) 259
Psp6I CCWGG 3 cut(s) 111, 155, 387
PspEI GGTNACC 1 cut(s) 401
PspFI CCCAGC 1 cut(s) 490
PspGI CCWGG 3 cut(s) 111, 155, 387
PspN4I GGNNCC 1 cut(s) 260
PspPI GGNCC 2 cut(s) 259, 340
PspPPI RGGWCCY 1 cut(s) 259
PvuI CGATCG 1 cut(s) 286
SaqAI TTAA 1 cut(s) 416
SatI GCNGC 1 cut(s) 339
Sau3AI GATC 1 cut(s) 283
Sau96I GGNCC 2 cut(s) 259, 340
SchI GAGTC 1 cut(s) 69
ScrFI CCNGG 8 cut(s) 113, 157, 193, 194, 257, 354, 389, 481
SduI GDGCHC 2 cut(s) 105, 149
SetI ASST 5 cut(s) 12, 303, 375, 393, 496
SinI GGWCC 1 cut(s) 259
SmaI CCCGGG 1 cut(s) 194
Sse9I AATT 2 cut(s) 35, 412
SsiI CCGC 9 cut(s) 138, 182, 209, 240, 338, 343, 397, 407, 432
SspMI CTAG 1 cut(s) 462
StyD4I CCNGG 8 cut(s) 111, 155, 191, 192, 255, 352, 387, 479
TaiI ACGT 1 cut(s) 12
TaqI TCGA 1 cut(s) 58
TasI AATT 2 cut(s) 35, 412
TauI GCSGC 1 cut(s) 341
TfiI GAWTC 2 cut(s) 43, 455
Tru1I TTAA 1 cut(s) 416
Tru9I TTAA 1 cut(s) 416
TseFI GTSAC 3 cut(s) 118, 162, 229
Tsp45I GTSAC 3 cut(s) 118, 162, 229
TspDTI ATGAA 1 cut(s) 17
TspMI CCCGGG 1 cut(s) 192
VpaK11BI GGWCC 1 cut(s) 259
XapI RAATTY 1 cut(s) 412
XceI RCATGY 1 cut(s) 313
XmaI CCCGGG 1 cut(s) 192
XspI CTAG 1 cut(s) 462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.