pycom2247g00030

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00002247
OG0002208
Physical Location & Seq
Reverse (-)
5347 .. 6761
1415 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom2247g00030.1

Sequence Viewer

Length: 936 bp
ATGTGCCCAATCCTCGCTTTATGCGGACAAGCAGCGAGGCTCCAGCTACGAATGCACATTGTCAATGGCATGTCGCAGGTTTCTCGGTCTAAAAGTTGGCTACCAAATCAAAGGGCAAAAGAAGTCTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGGCGGATGTTACTTTTAGGACTCCGCCGGCACCTTATGAGAAATCAAAGTTTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAAGAGTGGAGCCTGCGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACCAGCTATGCGGAGGTCATCCTCCGCGGCCAGCTTCTTAGAGGGACTATGGCCATCATTGCAATTGTTGGTCTTCAACGAGGAATTCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTCCTACCGATTGAATGGTCCGGTGAAGTGTTCGGATCGCGGCGACGCGGGTGGTTCGCTGCCCGCGACGTCGCGAGAAGTGAACCTGCGGAAGGATCATTGTCGAACCTGCGCAGCAGAACGACCCGAGAACCCGTTCCCACGTCGGGGGCCGGCGGGCCTTCGGGCTCGGCGTCCCCTTATCCCGGGAGCCCGCTCCTGGGCGTACCAACGAACACCGGCGCGTCTTTGCGCCAAGGAACCCGAACGAAAGAGCGCGCTCCCGCCGCCCCGGAAACGGTGCGCGCGCGGGCGGCGTCGTCGTCTTCTGTAAAGTCAAAACGACTCTCGGCAACGGATATCTCGGCTCTCGATCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

312

Amino Acids

33.05

Weight (kDa)

10.8

Isoelectric Point (pI)

59.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 648
Acc16I TGCGCA 1 cut(s) 689
Acc36I ACCTGC 3 cut(s) 67, 670, 693
AccB1I GGYRCC 2 cut(s) 220, 301
AccBSI CCGCTC 1 cut(s) 772
AclWI GGATC 3 cut(s) 196, 619, 679
AcoI YGGCCR 3 cut(s) 356, 451, 474
AcsI RAATTY 1 cut(s) 507
AcyI GRCGYC 3 cut(s) 645, 749, 872
AfaI GTAC 2 cut(s) 562, 783
AfiI CCNNNNNNNGG 8 cut(s) 190, 668, 722, 723, 761, 775, 776, 853
AgsI TTSAA 2 cut(s) 500, 590
AjiI CACGTC 1 cut(s) 720
AjnI CCWGG 2 cut(s) 183, 774
AluBI AGCT 4 cut(s) 46, 429, 457, 533
AluI AGCT 4 cut(s) 46, 429, 457, 533
Alw26I GTCTC 2 cut(s) 166, 402
AlwI GGATC 3 cut(s) 196, 619, 679
Ama87I CYCGRG 2 cut(s) 702, 761
AoxI GGCC 5 cut(s) 356, 451, 474, 726, 734
ApeKI GCWGC 3 cut(s) 32, 635, 690
ApoI RAATTY 1 cut(s) 507
Asp700I GAANNNNTTC 1 cut(s) 711
AspS9I GGNCC 3 cut(s) 594, 726, 734
AsuC2I CCSGG 4 cut(s) 253, 762, 763, 848
AsuHPI GGTGA 1 cut(s) 611
AvaI CYCGRG 2 cut(s) 702, 761
AvaII GGWCC 1 cut(s) 594
BaeGI GKGCMC 2 cut(s) 8, 304
BalI TGGCCA 1 cut(s) 476
BanI GGYRCC 2 cut(s) 220, 301
BanII GRGCYC 3 cut(s) 136, 746, 770
BbsI GAAGAC 3 cut(s) 145, 488, 873
BbvCI CCTCAGC 1 cut(s) 413
BbvI GCAGC 3 cut(s) 44, 622, 702
BccI CCATC 1 cut(s) 485
BceAI ACGGC 1 cut(s) 343
BcgI CGANNNNNNTGC 2 cut(s) 65, 99
BciT130I CCWGG 2 cut(s) 185, 776
BcnI CCSGG 4 cut(s) 253, 762, 763, 848
BcoDI GTCTC 2 cut(s) 166, 402
BfaI CTAG 2 cut(s) 158, 513
BfuAI ACCTGC 3 cut(s) 67, 670, 693
BisI GCNGC 7 cut(s) 33, 451, 617, 636, 691, 843, 870
BlsI GCNGC 7 cut(s) 34, 452, 618, 637, 692, 844, 871
Bme1390I CCNGG 6 cut(s) 185, 253, 762, 763, 776, 848
Bme18I GGWCC 1 cut(s) 594
BmeT110I CYCGRG 2 cut(s) 702, 761
BmgBI CACGTC 1 cut(s) 720
BmgT120I GGNCC 3 cut(s) 594, 726, 734
BmiI GGNNCC 8 cut(s) 41, 222, 250, 303, 318, 727, 767, 817
BmrFI CCNGG 6 cut(s) 185, 253, 762, 763, 776, 848
BmsI GCATC 1 cut(s) 166
BpiI GAAGAC 3 cut(s) 145, 488, 873
BpmI CTGGAG 1 cut(s) 26
Bpu10I CCTNAGC 1 cut(s) 413
BpuMI CCSGG 4 cut(s) 253, 762, 763, 848
Bsa29I ATCGAT 1 cut(s) 931
BsaHI GRCGYC 3 cut(s) 645, 749, 872
BsaI GGTCTC 1 cut(s) 402
BsaJI CCNNGG 7 cut(s) 184, 252, 448, 761, 775, 811, 846
BsaWI WCCGGW 1 cut(s) 596
Bsc4I CCNNNNNNNGG 8 cut(s) 190, 668, 722, 723, 761, 775, 776, 853
Bse118I RCCGGY 3 cut(s) 217, 728, 794
Bse3DI GCAATG 1 cut(s) 480
BseBI CCWGG 2 cut(s) 185, 776
BseCI ATCGAT 1 cut(s) 931
BseDI CCNNGG 7 cut(s) 184, 252, 448, 761, 775, 811, 846
BseGI GGATG 2 cut(s) 202, 441
BseLI CCNNNNNNNGG 8 cut(s) 190, 668, 722, 723, 761, 775, 776, 853
BseMI GCAATG 1 cut(s) 480
BseMII CTCAG 1 cut(s) 427
BsePI GCGCGC 3 cut(s) 832, 859, 861
BseSI GKGCMC 2 cut(s) 8, 304
BseXI GCAGC 3 cut(s) 44, 622, 702
Bsh1285I CGRYCG 1 cut(s) 931
BshFI GGCC 5 cut(s) 358, 453, 476, 728, 736
BshNI GGYRCC 2 cut(s) 220, 301
BshVI ATCGAT 1 cut(s) 931
BsiEI CGRYCG 1 cut(s) 931
BsiHKCI CYCGRG 2 cut(s) 702, 761
BsiSI CCGG 7 cut(s) 218, 252, 597, 729, 762, 795, 848
BslFI GGGAC 3 cut(s) 481, 533, 736
BslI CCNNNNNNNGG 8 cut(s) 190, 668, 722, 723, 761, 775, 776, 853
BsmAI GTCTC 2 cut(s) 166, 402
BsmFI GGGAC 3 cut(s) 481, 533, 736
BsmI GAATGC 1 cut(s) 57
BsnI GGCC 5 cut(s) 358, 453, 476, 728, 736
Bso31I GGTCTC 1 cut(s) 402
BsoBI CYCGRG 2 cut(s) 702, 761
Bsp1286I GDGCHC 5 cut(s) 8, 136, 304, 746, 770
Bsp1407I TGTACA 1 cut(s) 560
Bsp143I GATC 5 cut(s) 143, 188, 611, 671, 928
Bsp68I TCGCGA 1 cut(s) 650
BspANI GGCC 5 cut(s) 358, 453, 476, 728, 736
BspCNI CTCAG 1 cut(s) 426
BspDI ATCGAT 1 cut(s) 931
BspLI GGNNCC 8 cut(s) 41, 222, 250, 303, 318, 727, 767, 817
BspMI ACCTGC 3 cut(s) 67, 670, 693
BspPI GGATC 3 cut(s) 196, 619, 679
BspT107I GGYRCC 2 cut(s) 220, 301
BspTNI GGTCTC 1 cut(s) 402
BsrBI CCGCTC 1 cut(s) 772
BsrDI GCAATG 1 cut(s) 480
BsrFI RCCGGY 3 cut(s) 217, 728, 794
BsrGI TGTACA 1 cut(s) 560
BssAI RCCGGY 3 cut(s) 217, 728, 794
BssECI CCNNGG 7 cut(s) 184, 252, 448, 761, 775, 811, 846
BssHII GCGCGC 3 cut(s) 832, 859, 861
BssMI GATC 5 cut(s) 143, 188, 611, 671, 928
BssNI GRCGYC 3 cut(s) 645, 749, 872
BssT1I CCWWGG 1 cut(s) 811
Bst2UI CCWGG 2 cut(s) 185, 776
Bst4CI ACNGT 2 cut(s) 154, 856
BstACI GRCGYC 3 cut(s) 645, 749, 872
BstAUI TGTACA 1 cut(s) 560
BstDEI CTNAG 3 cut(s) 364, 413, 461
BstDSI CCRYGG 1 cut(s) 448
BstENI CCTNNNNNAGG 1 cut(s) 666
BstF5I GGATG 2 cut(s) 202, 441
BstKTI GATC 5 cut(s) 146, 191, 614, 674, 931
BstMAI GTCTC 2 cut(s) 166, 402
BstMBI GATC 5 cut(s) 143, 188, 611, 671, 928
BstMCI CGRYCG 1 cut(s) 931
BstMWI GCNNNNNNNGC 4 cut(s) 52, 482, 842, 869
BstNI CCWGG 2 cut(s) 185, 776
BstNSI RCATGY 1 cut(s) 73
BstSCI CCNGG 6 cut(s) 183, 251, 760, 761, 774, 846
BstSLI GKGCMC 2 cut(s) 8, 304
BstV1I GCAGC 3 cut(s) 44, 622, 702
BstV2I GAAGAC 3 cut(s) 145, 488, 873
Bsu15I ATCGAT 1 cut(s) 931
BsuRI GGCC 5 cut(s) 358, 453, 476, 728, 736
BsuTUI ATCGAT 1 cut(s) 931
BtgI CCRYGG 1 cut(s) 448
BtrI CACGTC 1 cut(s) 720
BtsCI GGATG 2 cut(s) 202, 441
BtuMI TCGCGA 1 cut(s) 650
BveI ACCTGC 3 cut(s) 67, 670, 693
Cfr10I RCCGGY 3 cut(s) 217, 728, 794
Cfr13I GGNCC 3 cut(s) 594, 726, 734
Cfr42I CCGCGG 1 cut(s) 451
Cfr9I CCCGGG 1 cut(s) 761
ClaI ATCGAT 1 cut(s) 931
CseI GACGC 4 cut(s) 630, 738, 789, 861
Csp6I GTAC 2 cut(s) 561, 782
CviAII CATG 2 cut(s) 70, 354
CviQI GTAC 2 cut(s) 561, 782
DdeI CTNAG 3 cut(s) 364, 413, 461
DpnI GATC 5 cut(s) 145, 190, 613, 673, 930
DpnII GATC 5 cut(s) 143, 188, 611, 671, 928
EaeI YGGCCR 3 cut(s) 356, 451, 474
EciI GGCGGA 2 cut(s) 204, 209
Eco130I CCWWGG 1 cut(s) 811
Eco24I GRGCYC 3 cut(s) 136, 746, 770
Eco31I GGTCTC 1 cut(s) 402
Eco32I GATATC 1 cut(s) 916
Eco47I GGWCC 1 cut(s) 594
Eco88I CYCGRG 2 cut(s) 702, 761
EcoNI CCTNNNNNAGG 1 cut(s) 666
EcoRI GAATTC 1 cut(s) 507
EcoRII CCWGG 2 cut(s) 183, 774
EcoRV GATATC 1 cut(s) 916
EcoT14I CCWWGG 1 cut(s) 811
EcoT38I GRGCYC 3 cut(s) 136, 746, 770
ErhI CCWWGG 1 cut(s) 811
FaeI CATG 2 cut(s) 73, 357
FaiI YATR 9 cut(s) 22, 71, 170, 228, 263, 341, 355, 432, 473
FaqI GGGAC 3 cut(s) 481, 533, 736
FatI CATG 2 cut(s) 69, 353
FauI CCCGC 6 cut(s) 617, 647, 725, 777, 847, 858
Fnu4HI GCNGC 7 cut(s) 33, 451, 617, 636, 691, 843, 870
FokI GGATG 2 cut(s) 209, 428
FriOI GRGCYC 3 cut(s) 136, 746, 770
Fsp4HI GCNGC 7 cut(s) 33, 451, 617, 636, 691, 843, 870
FspBI CTAG 2 cut(s) 158, 513
FspI TGCGCA 1 cut(s) 689
GluI GCNGC 7 cut(s) 33, 451, 617, 636, 691, 843, 870
GsuI CTGGAG 1 cut(s) 26
HaeIII GGCC 5 cut(s) 358, 453, 476, 728, 736
HapII CCGG 7 cut(s) 218, 252, 597, 729, 762, 795, 848
HgaI GACGC 4 cut(s) 630, 738, 789, 861
Hin1I GRCGYC 3 cut(s) 645, 749, 872
Hin1II CATG 2 cut(s) 73, 357
HincII GTYRAC 2 cut(s) 400, 541
HindII GTYRAC 2 cut(s) 400, 541
HinfI GANTC 4 cut(s) 211, 335, 524, 900
HpaI GTTAAC 1 cut(s) 400
HpaII CCGG 7 cut(s) 218, 252, 597, 729, 762, 795, 848
HphI GGTGA 1 cut(s) 611
Hpy166II GTNNAC 4 cut(s) 400, 541, 563, 659
Hpy188I TCNGA 2 cut(s) 148, 611
Hpy188III TCNNGA 3 cut(s) 332, 649, 926
Hpy8I GTNNAC 4 cut(s) 400, 541, 563, 659
Hpy99I CGWCG 7 cut(s) 577, 624, 647, 650, 724, 877, 880
HpyAV CCTTC 3 cut(s) 291, 662, 747
HpyCH4III ACNGT 2 cut(s) 154, 856
HpyCH4IV ACGT 3 cut(s) 546, 645, 719
HpyCH4V TGCA 3 cut(s) 55, 353, 485
HpyF10VI GCNNNNNNNGC 4 cut(s) 52, 482, 842, 869
HpyF3I CTNAG 3 cut(s) 364, 413, 461
HpySE526I ACGT 3 cut(s) 546, 645, 719
Hsp92I GRCGYC 3 cut(s) 645, 749, 872
Hsp92II CATG 2 cut(s) 73, 357
KroI GCCGGC 2 cut(s) 217, 728
KroNI GCCGGC 2 cut(s) 219, 730
KspAI GTTAAC 1 cut(s) 400
KspI CCGCGG 1 cut(s) 451
Kzo9I GATC 5 cut(s) 143, 188, 611, 671, 928
LmnI GCTCC 7 cut(s) 45, 316, 374, 582, 765, 777, 841
Lsp1109I GCAGC 3 cut(s) 44, 622, 702
LweI GCATC 1 cut(s) 166
MaeI CTAG 2 cut(s) 158, 513
MaeII ACGT 3 cut(s) 546, 645, 719
MaeIII GTNAC 1 cut(s) 199
MalI GATC 5 cut(s) 145, 190, 613, 673, 930
MauBI CGCGCGCG 1 cut(s) 861
MbiI CCGCTC 1 cut(s) 772
MboI GATC 5 cut(s) 143, 188, 611, 671, 928
MboII GAAGA 3 cut(s) 150, 488, 873
MfeI CAATTG 1 cut(s) 486
MhlI GDGCHC 5 cut(s) 8, 136, 304, 746, 770
MlsI TGGCCA 1 cut(s) 476
MluCI AATT 4 cut(s) 288, 391, 486, 507
MluNI TGGCCA 1 cut(s) 476
MlyI GAGTC 3 cut(s) 205, 533, 894
MnlI CCTC 7 cut(s) 23, 30, 422, 430, 455, 458, 497
Mox20I TGGCCA 1 cut(s) 476
MroNI GCCGGC 2 cut(s) 217, 728
MroXI GAANNNNTTC 1 cut(s) 711
MscI TGGCCA 1 cut(s) 476
MseI TTAA 3 cut(s) 282, 390, 399
Msp20I TGGCCA 1 cut(s) 476
MspA1I CMGCKG 1 cut(s) 450
MspI CCGG 7 cut(s) 218, 252, 597, 729, 762, 795, 848
MspR9I CCNGG 6 cut(s) 185, 253, 762, 763, 776, 848
MunI CAATTG 1 cut(s) 486
Mva1269I GAATGC 1 cut(s) 57
MvaI CCWGG 2 cut(s) 185, 776
MwoI GCNNNNNNNGC 4 cut(s) 52, 482, 842, 869
NaeI GCCGGC 2 cut(s) 219, 730
NciI CCSGG 4 cut(s) 253, 762, 763, 848
NdeII GATC 5 cut(s) 143, 188, 611, 671, 928
NgoMIV GCCGGC 2 cut(s) 217, 728
NlaIII CATG 2 cut(s) 73, 357
NlaIV GGNNCC 8 cut(s) 41, 222, 250, 303, 318, 727, 767, 817
NmeAIII GCCGAG 3 cut(s) 725, 884, 899
NruI TCGCGA 1 cut(s) 650
NsbI TGCGCA 1 cut(s) 689
NspI RCATGY 1 cut(s) 73
PauI GCGCGC 3 cut(s) 832, 859, 861
PcsI WCGNNNNNNNCGW 3 cut(s) 581, 639, 746
PctI GAATGC 1 cut(s) 57
PdiI GCCGGC 2 cut(s) 219, 730
PdmI GAANNNNTTC 1 cut(s) 711
PfeI GAWTC 1 cut(s) 335
PflFI GACNNNGTC 1 cut(s) 545
PkrI GCNGC 7 cut(s) 34, 452, 618, 637, 692, 844, 871
Ple19I CGATCG 1 cut(s) 931
PleI GAGTC 3 cut(s) 205, 532, 894
PpsI GAGTC 3 cut(s) 205, 532, 894
Psp6I CCWGG 2 cut(s) 183, 774
PspGI CCWGG 2 cut(s) 183, 774
PspN4I GGNNCC 8 cut(s) 41, 222, 250, 303, 318, 727, 767, 817
PspPI GGNCC 3 cut(s) 594, 726, 734
PsyI GACNNNGTC 1 cut(s) 545
PteI GCGCGC 3 cut(s) 832, 859, 861
PvuI CGATCG 1 cut(s) 931
RruI TCGCGA 1 cut(s) 650
RsaI GTAC 2 cut(s) 562, 783
RsaNI GTAC 2 cut(s) 561, 782
SacII CCGCGG 1 cut(s) 451
SaqAI TTAA 3 cut(s) 282, 390, 399
SatI GCNGC 7 cut(s) 33, 451, 617, 636, 691, 843, 870
Sau3AI GATC 5 cut(s) 143, 188, 611, 671, 928
Sau96I GGNCC 3 cut(s) 594, 726, 734
SchI GAGTC 3 cut(s) 205, 533, 894
ScrFI CCNGG 6 cut(s) 185, 253, 762, 763, 776, 848
SduI GDGCHC 5 cut(s) 8, 136, 304, 746, 770
SfaNI GCATC 1 cut(s) 166
Sfr303I CCGCGG 1 cut(s) 451
SgrAI CRCCGGYG 1 cut(s) 794
SgrBI CCGCGG 1 cut(s) 451
SinI GGWCC 1 cut(s) 594
SmaI CCCGGG 1 cut(s) 763
Sse9I AATT 4 cut(s) 288, 391, 486, 507
SspMI CTAG 2 cut(s) 158, 513
StyD4I CCNGG 6 cut(s) 183, 251, 760, 761, 774, 846
StyI CCWWGG 1 cut(s) 811
TaaI ACNGT 2 cut(s) 154, 856
TaiI ACGT 3 cut(s) 549, 648, 722
TaqI TCGA 4 cut(s) 136, 680, 927, 931
TaqII GACCGA 1 cut(s) 75
TasI AATT 4 cut(s) 288, 391, 486, 507
TatI WGTACW 1 cut(s) 560
TauI GCSGC 4 cut(s) 453, 619, 845, 872
TfiI GAWTC 1 cut(s) 335
Tru1I TTAA 3 cut(s) 282, 390, 399
Tru9I TTAA 3 cut(s) 282, 390, 399
TseI GCWGC 3 cut(s) 32, 635, 690
TspGWI ACGGA 3 cut(s) 309, 385, 926
TspMI CCCGGG 1 cut(s) 761
Tth111I GACNNNGTC 1 cut(s) 545
VpaK11BI GGWCC 1 cut(s) 594
XagI CCTNNNNNAGG 1 cut(s) 666
XapI RAATTY 1 cut(s) 507
XceI RCATGY 1 cut(s) 73
XmaI CCCGGG 1 cut(s) 761
XmnI GAANNNNTTC 1 cut(s) 711
XspI CTAG 2 cut(s) 158, 513
ZraI GACGTC 1 cut(s) 646
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.