pycom10g28970

Belongs to the helicase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0001547
Physical Location & Seq
Reverse (-)
29414359 .. 29417150
2792 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1056 bp
ATGGCCTACAGAGAATCACAAAGTTACATCCTAAGTACATGGCTCTTCAATATCCTCTTCTTTTCCCATATGGTGAAGATGGGTACAGAAAAGGACTTCCTTGGAATCCAAATTTTAGGGGGAAAAAACCAAAGAATGGGGGGGGTAACAATGAGAGCATTTTTGGGTTATCAAATTCAGGATAGACCAGGACAAAGTGACACCCTGTTAAAAGGCGGCAGATTGTTTCAACAATACTTAGTTGACGCATATGCGACTCTTGAAGAAGATCGGTTAGACTTCATTAGGTTAAATCAAGATTCTTTTAGAGCAGAAGGTCTTAAAGGAATTCATGAAGCTCTCAAAGCAGGAAATGCAAGCAGTTCTGGTGTTGGCAAGAGAGTTATTCTGCCAACTTCGTTTACTGGCAATGTTAGATATATGATAAATAATTATCAGGATGCTATGGCTATATGTCGCCACCATGGAAATCCTGATTTATTTATCACTTTCACCTGCAATGCTAAATGGCCTGAGATTATTGAAGATCTACGTGATAAACCTGGTTGTAAAGCTGAAGACAGGCCAGATTTAACTTCCAGAATTTTTAAAGCAAAACTTGACCATTTGATAAAATACCTCAAGTCAGGAAAACCTTTTGGTGTTGTCGAATCAGTGATTTACACGGTGGAGTTCCAAAAAAGAGGTCTTCCTCATTGTCATATCTTGCTCTGGGTTAATAAACATTATAAGTGTCATTCGCCTTATGATGTTGATTCTATCATTTCGGCTGAGATTCCTAATGAAGGATGTGACAAAGCTGGGTATGATGCAGTTTCGCAATACATGATACACGGCCCATGTGGTGTTGCGAATCAATTTTCACCTTGCATGAAAGAAAACAAATGTTCAAAAAAATTTCCTAAATCTTTCACAAGTGAAACCACTTTTTCCACAGAGGGATTCGTTGTATATAAGCGTCGAGATATAGAGAATCTGTTTTTCATACCTTTGGCCCTGTTTAACAATTTTTTTCTTAAAGCAAAACATGAGGCTTTCGAAAGCAAATCTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
Helitron_like_N Herpes_Helicase PIF1 REPA_OB_2

Protein Analysis

352

Amino Acids

39.93

Weight (kDa)

8.8

Isoelectric Point (pI)

45.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helitron_like_N PF14214 57 - 237 4.6e-56 Helitron helicase-like domain at N-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 729
AarI CACCTGC 1 cut(s) 503
Acc36I ACCTGC 1 cut(s) 503
AccB7I CCANNNNNTGG 1 cut(s) 136
AciI CCGC 1 cut(s) 216
AcsI RAATTY 5 cut(s) 111, 174, 327, 582, 896
AcuI CTGAAG 1 cut(s) 576
AfaI GTAC 2 cut(s) 37, 85
AfiI CCNNNNNNNGG 2 cut(s) 136, 785
AgsI TTSAA 5 cut(s) 49, 230, 263, 524, 891
AjnI CCWGG 2 cut(s) 187, 541
AluBI AGCT 3 cut(s) 338, 554, 800
AluI AGCT 3 cut(s) 338, 554, 800
AoxI GGCC 5 cut(s) 3, 509, 563, 835, 993
ApoI RAATTY 5 cut(s) 111, 174, 327, 582, 896
ArsI GACNNNNNNTTYG 2 cut(s) 670, 702
AspS9I GGNCC 2 cut(s) 836, 994
AsuHPI GGTGA 3 cut(s) 85, 484, 855
AsuII TTCGAA 1 cut(s) 1038
BbsI GAAGAC 2 cut(s) 564, 680
BccI CCATC 1 cut(s) 73
BceAI ACGGC 1 cut(s) 850
BciT130I CCWGG 2 cut(s) 189, 543
BfmI CTRYAG 1 cut(s) 7
BfuAI ACCTGC 1 cut(s) 503
BglII AGATCT 1 cut(s) 526
BisI GCNGC 1 cut(s) 217
BlsI GCNGC 1 cut(s) 218
Bme1390I CCNGG 2 cut(s) 189, 543
BmgT120I GGNCC 2 cut(s) 836, 994
BmrFI CCNGG 2 cut(s) 189, 543
BmsI GCATC 2 cut(s) 430, 799
BpiI GAAGAC 2 cut(s) 564, 680
Bpu14I TTCGAA 1 cut(s) 1038
BpuEI CTTGAG 1 cut(s) 605
BsaAI YACGTR 1 cut(s) 533
BsaJI CCNNGG 2 cut(s) 100, 463
Bsc4I CCNNNNNNNGG 2 cut(s) 136, 785
Bse1I ACTGG 1 cut(s) 409
Bse3DI GCAATG 2 cut(s) 415, 505
BseBI CCWGG 2 cut(s) 189, 543
BseDI CCNNGG 2 cut(s) 100, 463
BseGI GGATG 3 cut(s) 27, 445, 794
BseLI CCNNNNNNNGG 2 cut(s) 136, 785
BseMI GCAATG 2 cut(s) 415, 505
BseMII CTCAG 2 cut(s) 504, 762
BseNI ACTGG 1 cut(s) 409
BseYI CCCAGC 1 cut(s) 800
BshFI GGCC 5 cut(s) 5, 511, 565, 837, 995
BslI CCNNNNNNNGG 2 cut(s) 136, 785
BsnI GGCC 5 cut(s) 5, 511, 565, 837, 995
Bsp119I TTCGAA 1 cut(s) 1038
Bsp143I GATC 2 cut(s) 268, 526
Bsp19I CCATGG 1 cut(s) 463
BspACI CCGC 1 cut(s) 216
BspANI GGCC 5 cut(s) 5, 511, 565, 837, 995
BspCNI CTCAG 2 cut(s) 505, 763
BspHI TCATGA 1 cut(s) 331
BspMI ACCTGC 1 cut(s) 503
BspQI GCTCTTC 1 cut(s) 50
BspT104I TTCGAA 1 cut(s) 1038
BsrDI GCAATG 2 cut(s) 415, 505
BsrI ACTGG 1 cut(s) 409
BssECI CCNNGG 2 cut(s) 100, 463
BssMI GATC 2 cut(s) 268, 526
BssT1I CCWWGG 2 cut(s) 100, 463
Bst2UI CCWGG 2 cut(s) 189, 543
Bst4CI ACNGT 1 cut(s) 667
Bst6I CTCTTC 2 cut(s) 50, 62
BstAPI GCANNNNNTGC 1 cut(s) 353
BstBAI YACGTR 1 cut(s) 533
BstBI TTCGAA 1 cut(s) 1038
BstC8I GCNNGC 1 cut(s) 358
BstDEI CTNAG 4 cut(s) 32, 238, 513, 771
BstDSI CCRYGG 1 cut(s) 463
BstENI CCTNNNNNAGG 1 cut(s) 783
BstF5I GGATG 3 cut(s) 27, 445, 794
BstKTI GATC 2 cut(s) 271, 529
BstMBI GATC 2 cut(s) 268, 526
BstMWI GCNNNNNNNGC 2 cut(s) 344, 353
BstNI CCWGG 2 cut(s) 189, 543
BstSCI CCNGG 2 cut(s) 187, 541
BstSFI CTRYAG 1 cut(s) 7
BstV2I GAAGAC 2 cut(s) 564, 680
BstX2I RGATCY 1 cut(s) 526
BstYI RGATCY 1 cut(s) 526
BsuRI GGCC 5 cut(s) 5, 511, 565, 837, 995
BtgI CCRYGG 1 cut(s) 463
BtsCI GGATG 3 cut(s) 27, 445, 794
BtsIMutI CAGTG 1 cut(s) 660
BveI ACCTGC 1 cut(s) 503
Cac8I GCNNGC 1 cut(s) 358
CciI TCATGA 1 cut(s) 331
Cfr13I GGNCC 2 cut(s) 836, 994
CseI GACGC 2 cut(s) 254, 947
CsiI ACCWGGT 1 cut(s) 541
Csp6I GTAC 2 cut(s) 36, 84
CviAII CATG 7 cut(s) 39, 332, 464, 826, 840, 871, 1028
CviQI GTAC 2 cut(s) 36, 84
DdeI CTNAG 4 cut(s) 32, 238, 513, 771
DpnI GATC 2 cut(s) 270, 528
DpnII GATC 2 cut(s) 268, 526
DraI TTTAAA 1 cut(s) 589
Eam1104I CTCTTC 2 cut(s) 50, 62
EarI CTCTTC 2 cut(s) 50, 62
Eco130I CCWWGG 2 cut(s) 100, 463
Eco57I CTGAAG 1 cut(s) 576
EcoNI CCTNNNNNAGG 1 cut(s) 783
EcoRI GAATTC 1 cut(s) 327
EcoRII CCWGG 2 cut(s) 187, 541
EcoT14I CCWWGG 2 cut(s) 100, 463
ErhI CCWWGG 2 cut(s) 100, 463
FaeI CATG 7 cut(s) 42, 335, 467, 829, 843, 874, 1031
FalI AAGNNNNNCTT 2 cut(s) 582, 614
FatI CATG 7 cut(s) 38, 331, 463, 825, 839, 870, 1027
FauNDI CATATG 2 cut(s) 69, 250
Fnu4HI GCNGC 1 cut(s) 217
FokI GGATG 3 cut(s) 14, 452, 801
Fsp4HI GCNGC 1 cut(s) 217
GluI GCNGC 1 cut(s) 217
GsaI CCCAGC 1 cut(s) 804
HaeIII GGCC 5 cut(s) 5, 511, 565, 837, 995
HgaI GACGC 2 cut(s) 254, 947
Hin1II CATG 7 cut(s) 42, 335, 467, 829, 843, 874, 1031
HincII GTYRAC 1 cut(s) 244
HindII GTYRAC 1 cut(s) 244
HphI GGTGA 3 cut(s) 85, 484, 855
Hpy166II GTNNAC 2 cut(s) 244, 402
Hpy8I GTNNAC 2 cut(s) 244, 402
Hpy99I CGWCG 1 cut(s) 963
HpyAV CCTTC 2 cut(s) 308, 779
HpyCH4III ACNGT 1 cut(s) 667
HpyCH4IV ACGT 1 cut(s) 532
HpyCH4V TGCA 4 cut(s) 356, 498, 812, 870
HpyF10VI GCNNNNNNNGC 2 cut(s) 344, 353
HpyF3I CTNAG 4 cut(s) 32, 238, 513, 771
HpySE526I ACGT 1 cut(s) 532
Hsp92II CATG 7 cut(s) 42, 335, 467, 829, 843, 874, 1031
Kzo9I GATC 2 cut(s) 268, 526
LguI GCTCTTC 1 cut(s) 50
LweI GCATC 2 cut(s) 430, 799
MabI ACCWGGT 1 cut(s) 541
MaeII ACGT 1 cut(s) 532
MaeIII GTNAC 4 cut(s) 23, 145, 197, 791
MalI GATC 2 cut(s) 270, 528
MboI GATC 2 cut(s) 268, 526
MboII GAAGA 8 cut(s) 37, 49, 88, 275, 278, 536, 569, 680
MflI RGATCY 1 cut(s) 526
MluCI AATT 8 cut(s) 111, 174, 327, 430, 582, 857, 896, 1006
MlyI GAGTC 1 cut(s) 250
MnlI CCTC 6 cut(s) 65, 629, 677, 702, 931, 1024
MseI TTAA 8 cut(s) 209, 290, 321, 572, 588, 717, 1002, 1017
MspR9I CCNGG 2 cut(s) 189, 543
MvaI CCWGG 2 cut(s) 189, 543
MwoI GCNNNNNNNGC 2 cut(s) 344, 353
NcoI CCATGG 1 cut(s) 463
NdeI CATATG 2 cut(s) 69, 250
NdeII GATC 2 cut(s) 268, 526
NlaIII CATG 7 cut(s) 42, 335, 467, 829, 843, 874, 1031
NmuCI GTSAC 2 cut(s) 197, 791
NspV TTCGAA 1 cut(s) 1038
PagI TCATGA 1 cut(s) 331
PaqCI CACCTGC 1 cut(s) 503
PciSI GCTCTTC 1 cut(s) 50
PfeI GAWTC 9 cut(s) 14, 105, 299, 650, 755, 775, 853, 942, 973
PflMI CCANNNNNTGG 1 cut(s) 136
PkrI GCNGC 1 cut(s) 218
PleI GAGTC 1 cut(s) 250
PpsI GAGTC 1 cut(s) 250
Ppu21I YACGTR 1 cut(s) 533
PsiI TTATAA 1 cut(s) 729
Psp6I CCWGG 2 cut(s) 187, 541
PspFI CCCAGC 1 cut(s) 800
PspGI CCWGG 2 cut(s) 187, 541
PspPI GGNCC 2 cut(s) 836, 994
PsuI RGATCY 1 cut(s) 526
RsaI GTAC 2 cut(s) 37, 85
RsaNI GTAC 2 cut(s) 36, 84
SapI GCTCTTC 1 cut(s) 50
SaqAI TTAA 8 cut(s) 209, 290, 321, 572, 588, 717, 1002, 1017
SatI GCNGC 1 cut(s) 217
Sau3AI GATC 2 cut(s) 268, 526
Sau96I GGNCC 2 cut(s) 836, 994
SchI GAGTC 1 cut(s) 250
ScrFI CCNGG 2 cut(s) 189, 543
SexAI ACCWGGT 1 cut(s) 541
SfaNI GCATC 2 cut(s) 430, 799
SfcI CTRYAG 1 cut(s) 7
SfuI TTCGAA 1 cut(s) 1038
SmlI CTYRAG 1 cut(s) 620
SmoI CTYRAG 1 cut(s) 620
Sse9I AATT 8 cut(s) 111, 174, 327, 430, 582, 857, 896, 1006
SsiI CCGC 1 cut(s) 216
StyD4I CCNGG 2 cut(s) 187, 541
StyI CCWWGG 2 cut(s) 100, 463
TaaI ACNGT 1 cut(s) 667
TaiI ACGT 1 cut(s) 535
TaqI TCGA 3 cut(s) 648, 961, 1038
TasI AATT 8 cut(s) 111, 174, 327, 430, 582, 857, 896, 1006
TatI WGTACW 1 cut(s) 35
TauI GCSGC 1 cut(s) 219
TfiI GAWTC 9 cut(s) 14, 105, 299, 650, 755, 775, 853, 942, 973
Tru1I TTAA 8 cut(s) 209, 290, 321, 572, 588, 717, 1002, 1017
Tru9I TTAA 8 cut(s) 209, 290, 321, 572, 588, 717, 1002, 1017
TscAI CASTG 1 cut(s) 660
TseFI GTSAC 2 cut(s) 197, 791
Tsp45I GTSAC 2 cut(s) 197, 791
TspDTI ATGAA 6 cut(s) 271, 320, 348, 798, 887, 973
TspRI CASTG 1 cut(s) 660
Van91I CCANNNNNTGG 1 cut(s) 136
XagI CCTNNNNNAGG 1 cut(s) 783
XapI RAATTY 5 cut(s) 111, 174, 327, 582, 896
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.