pycom111g01560

Cystinosin homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0001326
Physical Location & Seq
Reverse (-)
973330 .. 975875
2546 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g01560.3

Sequence Viewer

Length: 867 bp
ATGGCGTTGTGGAATTCGATTCCGTTGGAAATCACATACGAAGTTCTGGGATGGGTGGCGTTCCTGAGTTGGACCGTCGGTGCCTACCCGCAAATCGTCTTAAATATCCGCCGGAAAAGTGTTGTGGGGCTGAACTTCGATTTCGTGGTGCTGAATTTGACAAAGCAATCCGCCTATCTCATTTACAACGCCAGTCTCTACTTCAGCTCCGCCGTCCAAAAGCAGTACTTTGAAAAGTACGGCAAAGAACAGATGATACCTGTTGCAGCAAATGATGTTGCTTTCTCTATTCATGCCGTTTTGATGACGGCAGTTACCCTGTGCCAAATTGCAATCTATGAACGCGGAAATCAGAAGGTCTCCAAGATTTCCTTTGGAATTGTTGCTGCCGTGTGGTTAGGTGCTGCAGTTTGTGTTTTCTTAGCTCTGCCAACCCATTCCTGGCTTTGGTTGATCTCAATTTTCAACTCAATTCAAGTTTTTATGACAGTCATCAAATATATTCCCCAGGCGTTCATGAACTTCCTGCGGAAGAGCACAGATGGATTCAGCATTGGCAACATTTTACTTGACTTTTCCGGAGGGGTGGCTAATTATGCACAAATGGCCGTGCAGTCTATAGATCAAGGTTCTTGGGTGAACTTCTATGGAAATATAGGAAAGACGCTGCTGTCTTTGATATCTATATTCTTTGACCTTATATTCATGAGTCAACACTTTGTGCTGTATCGCGGCAAGAGGGCATTGATCGCCCCTAAAATCATCAGTAAGGAGAGCACGGAACCACTTACCCCTAAAATCATCAGTAATGAGAGCACGGAACCACTTATCAAGTCTTCCGATGATCCAGTTTCAGAAAATGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 map04142
Pfam Domains
Protein Families
PQ-loop

Protein Analysis

289

Amino Acids

32.2

Weight (kDa)

8.72

Isoelectric Point (pI)

32.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 670
AccB1I GGYRCC 1 cut(s) 80
AccII CGCG 2 cut(s) 345, 732
AccIII TCCGGA 1 cut(s) 578
AciI CCGC 7 cut(s) 89, 109, 171, 210, 345, 529, 732
AclWI GGATC 1 cut(s) 839
AcoI YGGCCR 1 cut(s) 606
AcsI RAATTY 2 cut(s) 13, 154
AcuI CTGAAG 1 cut(s) 187
AdeI CACNNNGTG 1 cut(s) 721
AfaI GTAC 2 cut(s) 227, 239
AfiI CCNNNNNNNGG 2 cut(s) 441, 447
AgsI TTSAA 3 cut(s) 233, 466, 476
AjnI CCWGG 2 cut(s) 440, 507
AluBI AGCT 2 cut(s) 207, 425
AluI AGCT 2 cut(s) 207, 425
Alw21I GWGCWC 3 cut(s) 539, 779, 818
Alw26I GTCTC 2 cut(s) 200, 364
AlwI GGATC 1 cut(s) 839
Aor13HI TCCGGA 1 cut(s) 578
AoxI GGCC 1 cut(s) 606
ApeKI GCWGC 4 cut(s) 266, 386, 404, 667
ApoI RAATTY 2 cut(s) 13, 154
AspS9I GGNCC 1 cut(s) 72
AsuHPI GGTGA 1 cut(s) 649
AvaII GGWCC 1 cut(s) 72
BanI GGYRCC 1 cut(s) 80
BbsI GAAGAC 1 cut(s) 828
Bbv12I GWGCWC 3 cut(s) 539, 779, 818
BbvI GCAGC 4 cut(s) 278, 373, 391, 654
BccI CCATC 2 cut(s) 45, 536
BceAI ACGGC 6 cut(s) 197, 256, 281, 324, 374, 593
BciT130I CCWGG 2 cut(s) 442, 509
BcoDI GTCTC 2 cut(s) 200, 364
BfmI CTRYAG 2 cut(s) 405, 618
BisI GCNGC 5 cut(s) 267, 387, 405, 668, 733
BlsI GCNGC 5 cut(s) 268, 388, 406, 669, 734
BmcAI AGTACT 1 cut(s) 227
Bme1390I CCNGG 2 cut(s) 442, 509
Bme18I GGWCC 1 cut(s) 72
BmgT120I GGNCC 1 cut(s) 72
BmiI GGNNCC 3 cut(s) 82, 783, 822
BmrFI CCNGG 2 cut(s) 442, 509
BpiI GAAGAC 1 cut(s) 828
BsaI GGTCTC 1 cut(s) 364
BsaJI CCNNGG 1 cut(s) 507
BsaWI WCCGGW 1 cut(s) 578
BsaXI ACNNNNNCTCC 2 cut(s) 191, 221
Bsc4I CCNNNNNNNGG 2 cut(s) 441, 447
Bse1I ACTGG 2 cut(s) 192, 848
BseAI TCCGGA 1 cut(s) 578
BseBI CCWGG 2 cut(s) 442, 509
BseDI CCNNGG 1 cut(s) 507
BseGI GGATG 1 cut(s) 56
BseLI CCNNNNNNNGG 2 cut(s) 441, 447
BseMII CTCAG 1 cut(s) 56
BseNI ACTGG 2 cut(s) 192, 848
BseXI GCAGC 4 cut(s) 278, 373, 391, 654
BsgI GTGCAG 1 cut(s) 632
Bsh1236I CGCG 2 cut(s) 345, 732
BshFI GGCC 1 cut(s) 608
BshNI GGYRCC 1 cut(s) 80
BsiHKAI GWGCWC 3 cut(s) 539, 779, 818
BsiSI CCGG 2 cut(s) 112, 579
BslI CCNNNNNNNGG 2 cut(s) 441, 447
BsmAI GTCTC 2 cut(s) 200, 364
BsnI GGCC 1 cut(s) 608
Bso31I GGTCTC 1 cut(s) 364
Bsp1286I GDGCHC 3 cut(s) 539, 779, 818
Bsp13I TCCGGA 1 cut(s) 578
Bsp143I GATC 4 cut(s) 453, 622, 747, 844
BspACI CCGC 7 cut(s) 89, 109, 171, 210, 345, 529, 732
BspANI GGCC 1 cut(s) 608
BspCNI CTCAG 1 cut(s) 57
BspEI TCCGGA 1 cut(s) 578
BspFNI CGCG 2 cut(s) 345, 732
BspHI TCATGA 2 cut(s) 516, 705
BspLI GGNNCC 3 cut(s) 82, 783, 822
BspMAI CTGCAG 1 cut(s) 409
BspPI GGATC 1 cut(s) 839
BspQI GCTCTTC 1 cut(s) 527
BspT107I GGYRCC 1 cut(s) 80
BspTNI GGTCTC 1 cut(s) 364
BsrI ACTGG 2 cut(s) 192, 848
BssECI CCNNGG 1 cut(s) 507
BssMI GATC 4 cut(s) 453, 622, 747, 844
Bst2UI CCWGG 2 cut(s) 442, 509
Bst4CI ACNGT 2 cut(s) 76, 490
Bst6I CTCTTC 1 cut(s) 527
BstDEI CTNAG 2 cut(s) 65, 421
BstF5I GGATG 1 cut(s) 56
BstFNI CGCG 2 cut(s) 345, 732
BstKTI GATC 4 cut(s) 456, 625, 750, 847
BstMAI GTCTC 2 cut(s) 200, 364
BstMBI GATC 4 cut(s) 453, 622, 747, 844
BstMWI GCNNNNNNNGC 3 cut(s) 596, 605, 749
BstNI CCWGG 2 cut(s) 442, 509
BstSCI CCNGG 2 cut(s) 440, 507
BstSFI CTRYAG 2 cut(s) 405, 618
BstUI CGCG 2 cut(s) 345, 732
BstV1I GCAGC 4 cut(s) 278, 373, 391, 654
BstV2I GAAGAC 1 cut(s) 828
BsuRI GGCC 1 cut(s) 608
BtsCI GGATG 1 cut(s) 56
CciI TCATGA 2 cut(s) 516, 705
Cfr13I GGNCC 1 cut(s) 72
CseI GACGC 1 cut(s) 673
Csp6I GTAC 2 cut(s) 226, 238
CviAII CATG 3 cut(s) 293, 517, 706
CviJI RGCY 6 cut(s) 130, 207, 425, 445, 590, 608
CviKI_1 RGCY 6 cut(s) 130, 207, 425, 445, 590, 608
CviQI GTAC 2 cut(s) 226, 238
DdeI CTNAG 2 cut(s) 65, 421
DpnI GATC 4 cut(s) 455, 624, 749, 846
DpnII GATC 4 cut(s) 453, 622, 747, 844
DraIII CACNNNGTG 1 cut(s) 721
DrdI GACNNNNNNGTC 1 cut(s) 670
DseDI GACNNNNNNGTC 1 cut(s) 670
EaeI YGGCCR 1 cut(s) 606
Eam1104I CTCTTC 1 cut(s) 527
EarI CTCTTC 1 cut(s) 527
EciI GGCGGA 3 cut(s) 98, 160, 199
Eco31I GGTCTC 1 cut(s) 364
Eco32I GATATC 1 cut(s) 681
Eco47I GGWCC 1 cut(s) 72
Eco57I CTGAAG 1 cut(s) 187
EcoRI GAATTC 1 cut(s) 13
EcoRII CCWGG 2 cut(s) 440, 507
EcoRV GATATC 1 cut(s) 681
FaeI CATG 3 cut(s) 296, 520, 709
FalI AAGNNNNNCTT 4 cut(s) 212, 244, 356, 388
FatI CATG 3 cut(s) 292, 516, 705
FauI CCCGC 1 cut(s) 96
Fnu4HI GCNGC 5 cut(s) 267, 387, 405, 668, 733
FokI GGATG 1 cut(s) 63
Fsp4HI GCNGC 5 cut(s) 267, 387, 405, 668, 733
GluI GCNGC 5 cut(s) 267, 387, 405, 668, 733
HaeIII GGCC 1 cut(s) 608
HapII CCGG 2 cut(s) 112, 579
HgaI GACGC 1 cut(s) 673
Hin1II CATG 3 cut(s) 296, 520, 709
HincII GTYRAC 1 cut(s) 713
HindII GTYRAC 1 cut(s) 713
HinfI GANTC 3 cut(s) 19, 546, 709
HpaII CCGG 2 cut(s) 112, 579
HphI GGTGA 1 cut(s) 649
Hpy166II GTNNAC 2 cut(s) 640, 713
Hpy188I TCNGA 3 cut(s) 354, 841, 856
Hpy188III TCNNGA 4 cut(s) 64, 517, 579, 706
Hpy8I GTNNAC 2 cut(s) 640, 713
Hpy99I CGWCG 1 cut(s) 80
HpyAV CCTTC 1 cut(s) 349
HpyCH4III ACNGT 2 cut(s) 76, 490
HpyCH4V TGCA 5 cut(s) 266, 332, 407, 599, 613
HpyF10VI GCNNNNNNNGC 3 cut(s) 596, 605, 749
HpyF3I CTNAG 2 cut(s) 65, 421
Hsp92II CATG 3 cut(s) 296, 520, 709
Kpn2I TCCGGA 1 cut(s) 578
Kzo9I GATC 4 cut(s) 453, 622, 747, 844
LguI GCTCTTC 1 cut(s) 527
LmnI GCTCC 1 cut(s) 212
Lsp1109I GCAGC 4 cut(s) 278, 373, 391, 654
MaeIII GTNAC 1 cut(s) 313
MalI GATC 4 cut(s) 455, 624, 749, 846
MboI GATC 4 cut(s) 453, 622, 747, 844
MboII GAAGA 2 cut(s) 544, 828
MhlI GDGCHC 3 cut(s) 539, 779, 818
MluCI AATT 7 cut(s) 13, 154, 327, 378, 459, 471, 592
MlyI GAGTC 1 cut(s) 718
MmeI TCCRAC 2 cut(s) 6, 50
MnlI CCTC 2 cut(s) 575, 732
MroI TCCGGA 1 cut(s) 578
MseI TTAA 1 cut(s) 101
MspI CCGG 2 cut(s) 112, 579
MspR9I CCNGG 2 cut(s) 442, 509
MvaI CCWGG 2 cut(s) 442, 509
MvnI CGCG 2 cut(s) 345, 732
MwoI GCNNNNNNNGC 3 cut(s) 596, 605, 749
NdeII GATC 4 cut(s) 453, 622, 747, 844
NlaIII CATG 3 cut(s) 296, 520, 709
NlaIV GGNNCC 3 cut(s) 82, 783, 822
PagI TCATGA 2 cut(s) 516, 705
PciSI GCTCTTC 1 cut(s) 527
PfeI GAWTC 2 cut(s) 19, 546
PkrI GCNGC 5 cut(s) 268, 388, 406, 669, 734
PleI GAGTC 1 cut(s) 717
PpsI GAGTC 1 cut(s) 717
Psp6I CCWGG 2 cut(s) 440, 507
PspGI CCWGG 2 cut(s) 440, 507
PspN4I GGNNCC 3 cut(s) 82, 783, 822
PspPI GGNCC 1 cut(s) 72
PsrI GAACNNNNNNTAC 2 cut(s) 240, 272
PstI CTGCAG 1 cut(s) 409
RsaI GTAC 2 cut(s) 227, 239
RsaNI GTAC 2 cut(s) 226, 238
SapI GCTCTTC 1 cut(s) 527
SaqAI TTAA 1 cut(s) 101
SatI GCNGC 5 cut(s) 267, 387, 405, 668, 733
Sau3AI GATC 4 cut(s) 453, 622, 747, 844
Sau96I GGNCC 1 cut(s) 72
ScaI AGTACT 1 cut(s) 227
SchI GAGTC 1 cut(s) 718
ScrFI CCNGG 2 cut(s) 442, 509
SduI GDGCHC 3 cut(s) 539, 779, 818
SetI ASST 7 cut(s) 209, 262, 360, 403, 427, 631, 699
SfcI CTRYAG 2 cut(s) 405, 618
SinI GGWCC 1 cut(s) 72
Sse9I AATT 7 cut(s) 13, 154, 327, 378, 459, 471, 592
SsiI CCGC 7 cut(s) 89, 109, 171, 210, 345, 529, 732
StyD4I CCNGG 2 cut(s) 440, 507
TaaI ACNGT 2 cut(s) 76, 490
TaqI TCGA 2 cut(s) 17, 138
TasI AATT 7 cut(s) 13, 154, 327, 378, 459, 471, 592
TatI WGTACW 1 cut(s) 225
TauI GCSGC 1 cut(s) 735
TfiI GAWTC 2 cut(s) 19, 546
Tru1I TTAA 1 cut(s) 101
Tru9I TTAA 1 cut(s) 101
TseI GCWGC 4 cut(s) 266, 386, 404, 667
TspDTI ATGAA 5 cut(s) 281, 354, 505, 533, 694
TspGWI ACGGA 3 cut(s) 12, 794, 833
VpaK11BI GGWCC 1 cut(s) 72
XapI RAATTY 2 cut(s) 13, 154
XcmI CCANNNNNNNNNTGG 1 cut(s) 438
ZrmI AGTACT 1 cut(s) 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.