RLG00000022233

Cystinosin homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001326
Physical Location & Seq
Forward (+)
81849292 .. 81851567
2276 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022233

Sequence Viewer

Length: 828 bp
ATGGCGTCTTGGAATTCAGTCCCACTCGAAATCACATACGAAGTTCTGGGTTGGTTGGCCTTCATTTCATGGTCCATCAGCTTTTACCCACAAGTCATCTTAAACTTTCGCAGAAAAAGTGTTATTGGGTTGAACTTCGATTTCGTGCTACTGAATTTGACCAAGCACTCGTCGTATCTCATTTACAACGCCACTCTCTACTTCAGCTCCGCCGTTCAAAAACAGTACTTGGAGAAATATGGACTCGGAGAGATGATACCGGTAGCAGCAAATGATGTTGCGTTTTCTATTCATGCGGTTTTGCTGACAGCAATTACCTTGTTCCAGGTTGTGATCTACGAACGTGGAAGTCAGAAGGTGTCCAGGATTTCCATTGGAATTGTTGCTGCCGTTTGGTTAGGTGCTGCAGTTTGTTTCTTCGTAGCTTTGCCAACCCATTCTTGGCTCTGGCTCATTTCCATTTTCAACTCAATTCAAGTTTTTATGACAGCCATCAAATATATTCCTCAGGCAATCTTCAACTTCACGCGGAAGAGCACAGTTGGGTTTAGCATTGGCAACATTTTACTCGACTTTTCTGGAGGGGTGGCAAATTATGCACAAATGGGCGTGCAGTCCATAGATCAAGGTTCTTGGGTGAACTTTTATGGAAATATAGGGAAGACACTGCTGTCTTTGATATCAATCTTCTTTGACCTTCTCTTCATGGGTCAGCATTTCATACTGTATCCTGCCAAGGGAGCAACAATCTCTTCTAAAATAAGCAAGGAGGAGAGCACGGAGCCACTCGTTAAGTCATCTGATGACCCAGTATCTGAAAATGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 map04142
Pfam Domains
Protein Families
PQ-loop

Protein Analysis

276

Amino Acids

30.65

Weight (kDa)

7.79

Isoelectric Point (pI)

33.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PQ-loop PF04193 14 - 69 2.4e-18 PQ loop repeat
PQ-loop PF04193 152 - 204 2.6e-14 PQ loop repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 670
AccII CGCG 1 cut(s) 529
AciI CCGC 3 cut(s) 210, 296, 529
AcsI RAATTY 2 cut(s) 13, 154
AcuI CTGAAG 1 cut(s) 187
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 227
AfiI CCNNNNNNNGG 2 cut(s) 441, 737
AgeI ACCGGT 1 cut(s) 259
AgsI TTSAA 5 cut(s) 133, 218, 466, 476, 520
AjnI CCWGG 2 cut(s) 324, 362
AluBI AGCT 3 cut(s) 81, 207, 425
AluI AGCT 3 cut(s) 81, 207, 425
Alw21I GWGCWC 2 cut(s) 539, 779
AlwNI CAGNNNCTG 1 cut(s) 815
AoxI GGCC 1 cut(s) 57
ApeKI GCWGC 3 cut(s) 266, 386, 404
ApoI RAATTY 2 cut(s) 13, 154
AsiGI ACCGGT 1 cut(s) 259
AspS9I GGNCC 1 cut(s) 72
AsuHPI GGTGA 1 cut(s) 649
AvaII GGWCC 1 cut(s) 72
AxyI CCTNAGG 1 cut(s) 507
BbsI GAAGAC 1 cut(s) 668
Bbv12I GWGCWC 2 cut(s) 539, 779
BbvI GCAGC 3 cut(s) 278, 373, 391
BccI CCATC 2 cut(s) 83, 500
BceAI ACGGC 2 cut(s) 197, 374
BciT130I CCWGG 2 cut(s) 326, 364
BciVI GTATCC 1 cut(s) 738
BfmI CTRYAG 1 cut(s) 405
BfuI GTATCC 1 cut(s) 738
BisI GCNGC 3 cut(s) 267, 387, 405
BlsI GCNGC 3 cut(s) 268, 388, 406
BmcAI AGTACT 1 cut(s) 227
Bme1390I CCNGG 2 cut(s) 326, 364
Bme18I GGWCC 1 cut(s) 72
BmgT120I GGNCC 1 cut(s) 72
BmiI GGNNCC 1 cut(s) 783
BmrFI CCNGG 2 cut(s) 326, 364
BmrI ACTGGG 1 cut(s) 803
BmuI ACTGGG 1 cut(s) 803
BpiI GAAGAC 1 cut(s) 668
BpmI CTGGAG 1 cut(s) 600
BsaBI GATNNNNATC 1 cut(s) 683
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 1 cut(s) 735
BsaWI WCCGGW 1 cut(s) 259
BsaXI ACNNNNNCTCC 2 cut(s) 191, 221
Bsc4I CCNNNNNNNGG 2 cut(s) 441, 737
Bse118I RCCGGY 1 cut(s) 259
Bse1I ACTGG 1 cut(s) 809
Bse21I CCTNAGG 1 cut(s) 507
Bse8I GATNNNNATC 1 cut(s) 683
BseBI CCWGG 2 cut(s) 326, 364
BseDI CCNNGG 1 cut(s) 735
BseJI GATNNNNATC 1 cut(s) 683
BseLI CCNNNNNNNGG 2 cut(s) 441, 737
BseMII CTCAG 1 cut(s) 521
BseNI ACTGG 1 cut(s) 809
BseRI GAGGAG 1 cut(s) 785
BseXI GCAGC 3 cut(s) 278, 373, 391
BsgI GTGCAG 1 cut(s) 632
Bsh1236I CGCG 1 cut(s) 529
BshFI GGCC 1 cut(s) 59
BshTI ACCGGT 1 cut(s) 259
BsiHKAI GWGCWC 2 cut(s) 539, 779
BsiSI CCGG 1 cut(s) 260
BslFI GGGAC 1 cut(s) 5
BslI CCNNNNNNNGG 2 cut(s) 441, 737
BsmFI GGGAC 1 cut(s) 5
BsnI GGCC 1 cut(s) 59
Bsp1286I GDGCHC 2 cut(s) 539, 779
Bsp143I GATC 2 cut(s) 333, 622
BspACI CCGC 3 cut(s) 210, 296, 529
BspANI GGCC 1 cut(s) 59
BspCNI CTCAG 1 cut(s) 520
BspFNI CGCG 1 cut(s) 529
BspLI GGNNCC 1 cut(s) 783
BspMAI CTGCAG 1 cut(s) 409
BspQI GCTCTTC 1 cut(s) 527
BsrFI RCCGGY 1 cut(s) 259
BsrI ACTGG 1 cut(s) 809
BssAI RCCGGY 1 cut(s) 259
BssECI CCNNGG 1 cut(s) 735
BssMI GATC 2 cut(s) 333, 622
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 735
Bst2UI CCWGG 2 cut(s) 326, 364
Bst4CI ACNGT 3 cut(s) 225, 541, 726
Bst6I CTCTTC 3 cut(s) 527, 707, 757
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 596
BstC8I GCNNGC 1 cut(s) 611
BstDEI CTNAG 1 cut(s) 507
BstFNI CGCG 1 cut(s) 529
BstKTI GATC 2 cut(s) 336, 625
BstMBI GATC 2 cut(s) 333, 622
BstMWI GCNNNNNNNGC 2 cut(s) 596, 740
BstNI CCWGG 2 cut(s) 326, 364
BstSCI CCNGG 2 cut(s) 324, 362
BstSFI CTRYAG 1 cut(s) 405
BstUI CGCG 1 cut(s) 529
BstV1I GCAGC 3 cut(s) 278, 373, 391
BstV2I GAAGAC 1 cut(s) 668
Bsu36I CCTNAGG 1 cut(s) 507
BsuI GTATCC 1 cut(s) 738
BsuRI GGCC 1 cut(s) 59
BtsI GCAGTG 1 cut(s) 665
BtsIMutI CAGTG 1 cut(s) 665
Cac8I GCNNGC 1 cut(s) 611
CaiI CAGNNNCTG 1 cut(s) 815
Cfr10I RCCGGY 1 cut(s) 259
Cfr13I GGNCC 1 cut(s) 72
Csp6I GTAC 1 cut(s) 226
CspAI ACCGGT 1 cut(s) 259
CviAII CATG 3 cut(s) 69, 293, 706
CviJI RGCY 8 cut(s) 59, 81, 207, 425, 445, 451, 491, 784
CviKI_1 RGCY 8 cut(s) 59, 81, 207, 425, 445, 451, 491, 784
CviQI GTAC 1 cut(s) 226
DdeI CTNAG 1 cut(s) 507
DpnI GATC 2 cut(s) 335, 624
DpnII GATC 2 cut(s) 333, 622
DrdI GACNNNNNNGTC 1 cut(s) 670
DseDI GACNNNNNNGTC 1 cut(s) 670
Eam1104I CTCTTC 3 cut(s) 527, 707, 757
EarI CTCTTC 3 cut(s) 527, 707, 757
EciI GGCGGA 1 cut(s) 199
Eco130I CCWWGG 1 cut(s) 735
Eco32I GATATC 1 cut(s) 681
Eco47I GGWCC 1 cut(s) 72
Eco57I CTGAAG 1 cut(s) 187
Eco81I CCTNAGG 1 cut(s) 507
EcoRI GAATTC 1 cut(s) 13
EcoRII CCWGG 2 cut(s) 324, 362
EcoRV GATATC 1 cut(s) 681
EcoT14I CCWWGG 1 cut(s) 735
ErhI CCWWGG 1 cut(s) 735
FaeI CATG 3 cut(s) 72, 296, 709
FaqI GGGAC 1 cut(s) 5
FatI CATG 3 cut(s) 68, 292, 705
Fnu4HI GCNGC 3 cut(s) 267, 387, 405
Fsp4HI GCNGC 3 cut(s) 267, 387, 405
GluI GCNGC 3 cut(s) 267, 387, 405
GsuI CTGGAG 1 cut(s) 600
HaeIII GGCC 1 cut(s) 59
HapII CCGG 1 cut(s) 260
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 3 cut(s) 72, 296, 709
HinfI GANTC 1 cut(s) 243
HpaII CCGG 1 cut(s) 260
HphI GGTGA 1 cut(s) 649
Hpy166II GTNNAC 1 cut(s) 640
Hpy188I TCNGA 4 cut(s) 248, 354, 802, 817
Hpy188III TCNNGA 1 cut(s) 579
Hpy8I GTNNAC 1 cut(s) 640
Hpy99I CGWCG 1 cut(s) 175
HpyAV CCTTC 3 cut(s) 70, 349, 707
HpyCH4III ACNGT 3 cut(s) 225, 541, 726
HpyCH4IV ACGT 1 cut(s) 343
HpyCH4V TGCA 3 cut(s) 407, 599, 613
HpyF10VI GCNNNNNNNGC 2 cut(s) 596, 740
HpyF3I CTNAG 1 cut(s) 507
HpySE526I ACGT 1 cut(s) 343
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 3 cut(s) 72, 296, 709
Kzo9I GATC 2 cut(s) 333, 622
LguI GCTCTTC 1 cut(s) 527
LmnI GCTCC 3 cut(s) 212, 740, 781
Lsp1109I GCAGC 3 cut(s) 278, 373, 391
MaeII ACGT 1 cut(s) 343
MalI GATC 2 cut(s) 335, 624
MboI GATC 2 cut(s) 333, 622
MboII GAAGA 7 cut(s) 409, 508, 544, 673, 679, 694, 744
MhlI GDGCHC 2 cut(s) 539, 779
MluCI AATT 6 cut(s) 13, 154, 312, 378, 471, 592
MlyI GAGTC 1 cut(s) 237
MnlI CCTC 3 cut(s) 516, 575, 763
MseI TTAA 2 cut(s) 101, 792
MspI CCGG 1 cut(s) 260
MspR9I CCNGG 2 cut(s) 326, 364
MvaI CCWGG 2 cut(s) 326, 364
MvnI CGCG 1 cut(s) 529
MwoI GCNNNNNNNGC 2 cut(s) 596, 740
NdeII GATC 2 cut(s) 333, 622
NlaIII CATG 3 cut(s) 72, 296, 709
NlaIV GGNNCC 1 cut(s) 783
PciSI GCTCTTC 1 cut(s) 527
PfoI TCCNGGA 1 cut(s) 362
PinAI ACCGGT 1 cut(s) 259
PkrI GCNGC 3 cut(s) 268, 388, 406
PleI GAGTC 1 cut(s) 237
PpsI GAGTC 1 cut(s) 237
Psp6I CCWGG 2 cut(s) 324, 362
PspGI CCWGG 2 cut(s) 324, 362
PspN4I GGNNCC 1 cut(s) 783
PspPI GGNCC 1 cut(s) 72
PstI CTGCAG 1 cut(s) 409
PstNI CAGNNNCTG 1 cut(s) 815
RsaI GTAC 1 cut(s) 227
RsaNI GTAC 1 cut(s) 226
SapI GCTCTTC 1 cut(s) 527
SaqAI TTAA 2 cut(s) 101, 792
SatI GCNGC 3 cut(s) 267, 387, 405
Sau3AI GATC 2 cut(s) 333, 622
Sau96I GGNCC 1 cut(s) 72
ScaI AGTACT 1 cut(s) 227
SchI GAGTC 1 cut(s) 237
ScrFI CCNGG 2 cut(s) 326, 364
SduI GDGCHC 2 cut(s) 539, 779
SfcI CTRYAG 1 cut(s) 405
SinI GGWCC 1 cut(s) 72
Sse9I AATT 6 cut(s) 13, 154, 312, 378, 471, 592
SsiI CCGC 3 cut(s) 210, 296, 529
StyD4I CCNGG 2 cut(s) 324, 362
StyI CCWWGG 1 cut(s) 735
TaaI ACNGT 3 cut(s) 225, 541, 726
TaiI ACGT 1 cut(s) 346
TaqI TCGA 3 cut(s) 27, 138, 570
TasI AATT 6 cut(s) 13, 154, 312, 378, 471, 592
TatI WGTACW 1 cut(s) 225
Tru1I TTAA 2 cut(s) 101, 792
Tru9I TTAA 2 cut(s) 101, 792
TscAI CASTG 1 cut(s) 672
TseI GCWGC 3 cut(s) 266, 386, 404
TspDTI ATGAA 5 cut(s) 52, 57, 281, 694, 709
TspGWI ACGGA 1 cut(s) 794
TspRI CASTG 1 cut(s) 672
VpaK11BI GGWCC 1 cut(s) 72
XapI RAATTY 2 cut(s) 13, 154
XcmI CCANNNNNNNNNTGG 1 cut(s) 438
ZrmI AGTACT 1 cut(s) 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.