Rw2G053600

Cystinosin homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001326
Physical Location & Seq
Forward (+)
81342182 .. 81347411
5230 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G053600.1

Sequence Viewer

Length: 801 bp
ATGGCGTCGTGGAATTCGATTCAGCTGGAGATCGCATACAGAGTTCTGGGGTGGTTGACTTTCACTTTTTGGGCCGTCGGCTTCTACCCTCAAGTGTTCTTGAATTTCCGTCGAAAAAGTGTTGTGGGGCTGAGCATCGATTTTGTGGTACTGAATTTCACGAAGCACTCGTCGTATCTCATTTACAACGCCACTCTCTACTTCAGCTCCGCCGCGCGTTCAGAAATGATACCTGTAGCTGCAAATGATGTTGCTTTCTCTGTTCATGCCGTTTTTGTAACATCAATTATCCTTTTCCAAATTGCAATCTATGAACGAGGAAGTCAGAAAGTATCCAAGATTGCCATTGGAATCGTCTTTGGTGTGTGGCTATTTGCTGCAGTTTGTTTCTTGGTGGCTCTGCCAACTCATTCTTGGCTTTGGCTCATCTCTATCTTCAAGTATATAAGATACACATACATGAACTTCATAAGAAAGAGCACAGATGGATTAAGTATTTTCTTTATTCTCCTTGATCTTTCGGCACTACTAGAAATTTCCCCATTAGGGACTAACTTATATGGATTCATAATAACTTGTTTTGAATCTGTGCCCGCAGATTCTTGGGTGAACTTTTATGGAAACATAGGGAAGACACTGCTATCTTTGATATGTGTATCATTTGATCTTCTTTTCATTTGCCAACATTATGTTCTGTATACTTCCAAGAATGCATTGATATCTCCTAAAATGATCACCAGCAAGGAGGACTTGGAGCCCCTTATCAGTTCTGATAATCACCAAGTGTCTGAAAATGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 map04142
Pfam Domains
Protein Families
PQ-loop

Protein Analysis

266

Amino Acids

30.19

Weight (kDa)

7.67

Isoelectric Point (pI)

33.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PQ-loop PF04193 14 - 69 8.9e-18 PQ loop repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 698
AccII CGCG 2 cut(s) 215, 217
AciI CCGC 3 cut(s) 210, 213, 594
AcsI RAATTY 4 cut(s) 13, 103, 154, 534
AcuI CTGAAG 1 cut(s) 187
AcyI GRCGYC 1 cut(s) 5
AdeI CACNNNGTG 1 cut(s) 784
AfaI GTAC 1 cut(s) 150
AfiI CCNNNNNNNGG 1 cut(s) 546
AgsI TTSAA 3 cut(s) 103, 439, 584
AjuI GAANNNNNNNTTGG 2 cut(s) 675, 707
AluBI AGCT 3 cut(s) 25, 207, 239
AluI AGCT 3 cut(s) 25, 207, 239
Alw21I GWGCWC 1 cut(s) 482
AoxI GGCC 1 cut(s) 72
ApeKI GCWGC 2 cut(s) 239, 377
ApoI RAATTY 4 cut(s) 13, 103, 154, 534
ArsI GACNNNNNNTTYG 2 cut(s) 155, 187
AspLEI GCGC 1 cut(s) 217
AspS9I GGNCC 1 cut(s) 72
AsuHPI GGTGA 3 cut(s) 619, 727, 770
BaeGI GKGCMC 1 cut(s) 594
BanII GRGCYC 1 cut(s) 759
BbsI GAAGAC 1 cut(s) 638
Bbv12I GWGCWC 1 cut(s) 482
BbvI GCAGC 2 cut(s) 226, 364
BccI CCATC 1 cut(s) 479
BceAI ACGGC 2 cut(s) 59, 254
BciVI GTATCC 1 cut(s) 343
BclI TGATCA 1 cut(s) 732
BfaI CTAG 2 cut(s) 530, 799
BfmI CTRYAG 2 cut(s) 234, 378
BfuI GTATCC 1 cut(s) 343
BisI GCNGC 3 cut(s) 213, 240, 378
BlpI GCTNAGC 1 cut(s) 131
BlsI GCNGC 3 cut(s) 214, 241, 379
BmgT120I GGNCC 1 cut(s) 72
BmiI GGNNCC 1 cut(s) 756
BmsI GCATC 1 cut(s) 144
BpiI GAAGAC 1 cut(s) 638
BpmI CTGGAG 1 cut(s) 47
Bpu1102I GCTNAGC 1 cut(s) 131
BpuEI CTTGAG 1 cut(s) 75
Bsa29I ATCGAT 1 cut(s) 138
BsaHI GRCGYC 1 cut(s) 5
BsaXI ACNNNNNCTCC 2 cut(s) 191, 221
Bsc4I CCNNNNNNNGG 1 cut(s) 546
BseCI ATCGAT 1 cut(s) 138
BseLI CCNNNNNNNGG 1 cut(s) 546
BseMII CTCAG 1 cut(s) 122
BseSI GKGCMC 1 cut(s) 594
BseXI GCAGC 2 cut(s) 226, 364
Bsh1236I CGCG 2 cut(s) 215, 217
BshFI GGCC 1 cut(s) 74
BshVI ATCGAT 1 cut(s) 138
BsiHKAI GWGCWC 1 cut(s) 482
BslFI GGGAC 1 cut(s) 562
BslI CCNNNNNNNGG 1 cut(s) 546
BsmFI GGGAC 1 cut(s) 562
BsmI GAATGC 1 cut(s) 715
BsnI GGCC 1 cut(s) 74
Bsp1286I GDGCHC 3 cut(s) 482, 594, 759
Bsp143I GATC 4 cut(s) 30, 514, 664, 732
Bsp1720I GCTNAGC 1 cut(s) 131
BspACI CCGC 3 cut(s) 210, 213, 594
BspANI GGCC 1 cut(s) 74
BspCNI CTCAG 1 cut(s) 123
BspDI ATCGAT 1 cut(s) 138
BspFNI CGCG 2 cut(s) 215, 217
BspLI GGNNCC 1 cut(s) 756
BspMAI CTGCAG 1 cut(s) 382
BssMI GATC 4 cut(s) 30, 514, 664, 732
BssNAI GTATAC 1 cut(s) 699
BssNI GRCGYC 1 cut(s) 5
Bst1107I GTATAC 1 cut(s) 699
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 1 cut(s) 594
BstDEI CTNAG 1 cut(s) 131
BstFNI CGCG 2 cut(s) 215, 217
BstHHI GCGC 1 cut(s) 217
BstKTI GATC 4 cut(s) 33, 517, 667, 735
BstMBI GATC 4 cut(s) 30, 514, 664, 732
BstSFI CTRYAG 2 cut(s) 234, 378
BstSLI GKGCMC 1 cut(s) 594
BstUI CGCG 2 cut(s) 215, 217
BstV1I GCAGC 2 cut(s) 226, 364
BstV2I GAAGAC 1 cut(s) 638
BstZ17I GTATAC 1 cut(s) 699
Bsu15I ATCGAT 1 cut(s) 138
BsuI GTATCC 1 cut(s) 343
BsuRI GGCC 1 cut(s) 74
BsuTUI ATCGAT 1 cut(s) 138
BtsI GCAGTG 1 cut(s) 635
BtsIMutI CAGTG 1 cut(s) 635
Cac8I GCNNGC 1 cut(s) 594
CfoI GCGC 1 cut(s) 217
Cfr13I GGNCC 1 cut(s) 72
ClaI ATCGAT 1 cut(s) 138
Csp6I GTAC 1 cut(s) 149
CviAII CATG 2 cut(s) 266, 460
CviQI GTAC 1 cut(s) 149
DdeI CTNAG 1 cut(s) 131
DpnI GATC 4 cut(s) 32, 516, 666, 734
DpnII GATC 4 cut(s) 30, 514, 664, 732
DraIII CACNNNGTG 1 cut(s) 784
EciI GGCGGA 1 cut(s) 199
Eco24I GRGCYC 1 cut(s) 759
Eco32I GATATC 1 cut(s) 720
Eco57I CTGAAG 1 cut(s) 187
EcoRI GAATTC 1 cut(s) 13
EcoRV GATATC 1 cut(s) 720
EcoT22I ATGCAT 1 cut(s) 715
EcoT38I GRGCYC 1 cut(s) 759
FaeI CATG 2 cut(s) 269, 463
FalI AAGNNNNNCTT 2 cut(s) 734, 766
FaqI GGGAC 1 cut(s) 562
FatI CATG 2 cut(s) 265, 459
FauI CCCGC 1 cut(s) 601
FbaI TGATCA 1 cut(s) 732
FblI GTMKAC 1 cut(s) 698
Fnu4HI GCNGC 3 cut(s) 213, 240, 378
FriOI GRGCYC 1 cut(s) 759
Fsp4HI GCNGC 3 cut(s) 213, 240, 378
FspBI CTAG 2 cut(s) 530, 799
GlaI GCGC 1 cut(s) 216
GluI GCNGC 3 cut(s) 213, 240, 378
GsuI CTGGAG 1 cut(s) 47
HaeIII GGCC 1 cut(s) 74
HhaI GCGC 1 cut(s) 217
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 2 cut(s) 269, 463
Hin6I GCGC 1 cut(s) 215
HinP1I GCGC 1 cut(s) 215
HincII GTYRAC 1 cut(s) 57
HindII GTYRAC 1 cut(s) 57
HinfI GANTC 5 cut(s) 19, 351, 564, 584, 599
HphI GGTGA 3 cut(s) 619, 727, 770
Hpy166II GTNNAC 3 cut(s) 57, 610, 699
Hpy188I TCNGA 4 cut(s) 223, 327, 772, 790
Hpy188III TCNNGA 2 cut(s) 100, 160
Hpy8I GTNNAC 3 cut(s) 57, 610, 699
Hpy99I CGWCG 4 cut(s) 10, 80, 114, 175
HpyCH4V TGCA 4 cut(s) 242, 305, 380, 713
HpyF3I CTNAG 1 cut(s) 131
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 2 cut(s) 269, 463
HspAI GCGC 1 cut(s) 215
Ksp22I TGATCA 1 cut(s) 732
Kzo9I GATC 4 cut(s) 30, 514, 664, 732
LmnI GCTCC 2 cut(s) 212, 754
LpnPI CCDG 4 cut(s) 11, 32, 246, 751
Lsp1109I GCAGC 2 cut(s) 226, 364
LweI GCATC 1 cut(s) 144
MaeI CTAG 2 cut(s) 530, 799
MaeIII GTNAC 1 cut(s) 277
MalI GATC 4 cut(s) 32, 516, 666, 734
MboI GATC 4 cut(s) 30, 514, 664, 732
MboII GAAGA 3 cut(s) 427, 643, 659
MhlI GDGCHC 3 cut(s) 482, 594, 759
MluCI AATT 6 cut(s) 13, 103, 154, 285, 300, 534
MnlI CCTC 3 cut(s) 99, 311, 739
Mph1103I ATGCAT 1 cut(s) 715
MseI TTAA 1 cut(s) 491
MslI CAYNNNNRTG 1 cut(s) 458
MspA1I CMGCKG 1 cut(s) 25
Mva1269I GAATGC 1 cut(s) 715
MvnI CGCG 2 cut(s) 215, 217
NdeII GATC 4 cut(s) 30, 514, 664, 732
NlaIII CATG 2 cut(s) 269, 463
NlaIV GGNNCC 1 cut(s) 756
NsiI ATGCAT 1 cut(s) 715
PcsI WCGNNNNNNNCGW 2 cut(s) 14, 167
PctI GAATGC 1 cut(s) 715
PfeI GAWTC 5 cut(s) 19, 351, 564, 584, 599
PkrI GCNGC 3 cut(s) 214, 241, 379
PspN4I GGNNCC 1 cut(s) 756
PspPI GGNCC 1 cut(s) 72
PstI CTGCAG 1 cut(s) 382
PvuII CAGCTG 1 cut(s) 25
RsaI GTAC 1 cut(s) 150
RsaNI GTAC 1 cut(s) 149
RseI CAYNNNNRTG 1 cut(s) 458
SaqAI TTAA 1 cut(s) 491
SatI GCNGC 3 cut(s) 213, 240, 378
Sau3AI GATC 4 cut(s) 30, 514, 664, 732
Sau96I GGNCC 1 cut(s) 72
SduI GDGCHC 3 cut(s) 482, 594, 759
SetI ASST 4 cut(s) 27, 209, 235, 241
SfaNI GCATC 1 cut(s) 144
SfcI CTRYAG 2 cut(s) 234, 378
SmiMI CAYNNNNRTG 1 cut(s) 458
SmlI CTYRAG 1 cut(s) 90
SmoI CTYRAG 1 cut(s) 90
Sse9I AATT 6 cut(s) 13, 103, 154, 285, 300, 534
SsiI CCGC 3 cut(s) 210, 213, 594
SspMI CTAG 2 cut(s) 530, 799
TaqI TCGA 3 cut(s) 17, 112, 138
TasI AATT 6 cut(s) 13, 103, 154, 285, 300, 534
TauI GCSGC 1 cut(s) 215
TfiI GAWTC 5 cut(s) 19, 351, 564, 584, 599
Tru1I TTAA 1 cut(s) 491
Tru9I TTAA 1 cut(s) 491
TscAI CASTG 1 cut(s) 642
TseI GCWGC 2 cut(s) 239, 377
TspDTI ATGAA 6 cut(s) 254, 327, 457, 476, 556, 664
TspGWI ACGGA 1 cut(s) 98
TspRI CASTG 1 cut(s) 642
XapI RAATTY 4 cut(s) 13, 103, 154, 534
XcmI CCANNNNNNNNNTGG 1 cut(s) 411
XmiI GTMKAC 1 cut(s) 698
XspI CTAG 2 cut(s) 530, 799
Zsp2I ATGCAT 1 cut(s) 715
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.