Rorug02G0573300

Cystinosin homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001326
Physical Location & Seq
Forward (+)
69907016 .. 69909196
2181 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0573300.1

Sequence Viewer

Length: 1353 bp
ATGGTCAATCCTCATGTTTTGGTGGTACCTTATCCAGCACAAGGCCATGTAATTCCTTTGCTTGAGCTTTCAAGATGCCTAGTTGAACATGGTGTCAAGGTTACATTCGTAAATACCGAGCACGTTCATATGCAAATGAAAGAAGCAATGGCAATGGAGGAAGATACAGGGAGTAAAATTCATCAGGTGTTTATCACTGTTGGTTCGGAAGATGGGAAATACTCATCAGGGAAGTTGACTGAAGCAATTTTGAGGGTCTTGCCCCGAAAATTGGAGGAGCTTATTGAAGATATTAATGGATCAAATGGCGAGAGTATTACATGTGTTCTTGTTGATCAGACTCTTGGATGGGCCCTGGAAATCGCGGAGAAGAAGGCGATTAAGCGAGCTGCCTTCTGCCCAGCTGCAGCTGCACTCTTGGTACTTGGGTTAAGCATCCCAAAGCTGATTGATGATGGAGTTATCGACAATGAAGGAACTCCCACAAAGAAACAAGTGATTAAGCTATCACCAGATATGCCTGCCATGAACACGCAGGAGTTTGCGTGGGCATGCCTCGGACACAAGGCTATGCAGAAAAATGTGTTTGAGCTTATGCTCAGAAACAACAAATCAAGGAAATCAGCAGAGTGGTTACTTTGCAACTCATCCTATGACCTTGAGCCTGCAGCATTTACCATGACTCCACACATTCTACCAATAGGTCCACTTTTGGCAAGCAATCGGCTCAAACTAGACTTTGCAGGAGAAGACTCTTGCTTGACTTGGCTTGATCAACAAGCAACACAGTCTGTAGTGTATGTTGCTTTTGGTAGCTTAGCAGTTTTTAATCCAATACAGTTCAAAGAATTGGCCCTGGGGCTAGAACTCTCCAATAGACCATTCTTGTGGGTGGTGAGACCTGATATGATACACGCTGAAAATGAAGCCTACCTGAAAGAATTCAATGACAGGGTAGCTACTCAAGGACTGATAGTAGCTTGGGCACCTCAACAGAGGGTTCTGTCCCATCGCTCCATCGCTTGTTTTGTGAGCCATTGTGGTTGGAATTCAATCATGGAAGGTTTAGGATATGGAGTCCCTTTCTTATGCTGGCCTTACTTTGCTGATCAGTTTCTTAATCGAACCTACATTTCAGAGATTTGGAAGGTTGGTTTGGGGTTTGAAAGAGATGAAAGTGGTATCATAACAAGCAGAGAAATCAAAAATAAGGTGGAGCAGCTGCTAGGAAATGGGGAAATAAAAGCAAGGGCTTTCGATATCAAGAAACTGGTTATGGATGGCATCAAAGAAGGCGGTGGCTCAAACAAGAATTTCAAGGATTTTGTTGAATGGATAAAGGGATTGGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 map04142
Pfam Domains
Protein Families
PQ-loop

Protein Analysis

450

Amino Acids

50.17

Weight (kDa)

5.94

Isoelectric Point (pI)

32.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_N PF26168 6 - 50 1.1e-06 Glycosyltransferase, N-terminal domain
UDPGT PF00201 251 - 420 8.6e-21 UDP-glucoronosyl and UDP-glucosyl transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 25
AccB1I GGYRCC 2 cut(s) 25, 985
AccII CGCG 1 cut(s) 365
AciI CCGC 2 cut(s) 365, 1296
AclWI GGATC 1 cut(s) 307
AcsI RAATTY 4 cut(s) 177, 941, 1048, 1312
AcuI CTGAAG 1 cut(s) 261
AfaI GTAC 2 cut(s) 27, 423
AfiI CCNNNNNNNGG 2 cut(s) 41, 271
AflIII ACRYGT 1 cut(s) 320
AgsI TTSAA 9 cut(s) 72, 86, 287, 844, 946, 1053, 1166, 1318, 1331
AjnI CCWGG 2 cut(s) 354, 855
AjuI GAANNNNNNNTTGG 4 cut(s) 866, 898, 1139, 1171
Alw21I GWGCWC 1 cut(s) 123
Alw26I GTCTC 1 cut(s) 892
AlwI GGATC 1 cut(s) 307
AoxI GGCC 4 cut(s) 43, 351, 852, 1094
ApaI GGGCCC 1 cut(s) 355
ApeKI GCWGC 7 cut(s) 389, 404, 407, 410, 668, 1219, 1222
ApoI RAATTY 4 cut(s) 177, 941, 1048, 1312
AseI ATTAAT 1 cut(s) 294
Asp700I GAANNNNTTC 1 cut(s) 941
Asp718I GGTACC 1 cut(s) 25
AspS9I GGNCC 4 cut(s) 351, 352, 704, 853
AsuHPI GGTGA 2 cut(s) 501, 907
AvaII GGWCC 1 cut(s) 704
BaeGI GKGCMC 2 cut(s) 355, 988
BaeI ACNNNNGTAYC 2 cut(s) 413, 446
BanI GGYRCC 2 cut(s) 25, 985
BanII GRGCYC 1 cut(s) 355
BbsI GAAGAC 1 cut(s) 756
Bbv12I GWGCWC 1 cut(s) 123
BbvI GCAGC 7 cut(s) 376, 391, 397, 419, 680, 1209, 1231
BccI CCATC 6 cut(s) 206, 342, 449, 1017, 1025, 1274
BciT130I CCWGG 2 cut(s) 356, 857
BclI TGATCA 3 cut(s) 334, 772, 1108
BcoDI GTCTC 1 cut(s) 892
BfaI CTAG 4 cut(s) 80, 734, 863, 1226
BfmI CTRYAG 3 cut(s) 405, 666, 792
BisI GCNGC 7 cut(s) 390, 405, 408, 411, 669, 1220, 1223
BlpI GCTNAGC 1 cut(s) 817
BlsI GCNGC 7 cut(s) 391, 406, 409, 412, 670, 1221, 1224
Bme1390I CCNGG 2 cut(s) 356, 857
Bme18I GGWCC 1 cut(s) 704
BmgT120I GGNCC 4 cut(s) 351, 352, 704, 853
BmiI GGNNCC 3 cut(s) 27, 353, 987
BmrFI CCNGG 2 cut(s) 356, 857
BmsI GCATC 3 cut(s) 65, 444, 1293
BpiI GAAGAC 1 cut(s) 756
Bpu1102I GCTNAGC 1 cut(s) 817
BpuEI CTTGAG 3 cut(s) 83, 680, 948
BsaI GGTCTC 1 cut(s) 892
BsaJI CCNNGG 4 cut(s) 354, 556, 855, 856
BsaXI ACNNNNNCTCC 4 cut(s) 530, 560, 667, 697
Bsc4I CCNNNNNNNGG 2 cut(s) 41, 271
Bse1I ACTGG 1 cut(s) 1275
Bse3DI GCAATG 2 cut(s) 153, 159
BseBI CCWGG 2 cut(s) 356, 857
BseDI CCNNGG 4 cut(s) 354, 556, 855, 856
BseGI GGATG 4 cut(s) 353, 435, 647, 1285
BseLI CCNNNNNNNGG 2 cut(s) 41, 271
BseMI GCAATG 2 cut(s) 153, 159
BseMII CTCAG 1 cut(s) 613
BseNI ACTGG 1 cut(s) 1275
BseRI GAGGAG 1 cut(s) 290
BseSI GKGCMC 2 cut(s) 355, 988
BseXI GCAGC 7 cut(s) 376, 391, 397, 419, 680, 1209, 1231
BseYI CCCAGC 1 cut(s) 400
BsgI GTGCAG 1 cut(s) 396
Bsh1236I CGCG 1 cut(s) 365
BshFI GGCC 4 cut(s) 45, 353, 854, 1096
BshNI GGYRCC 2 cut(s) 25, 985
BsiHKAI GWGCWC 1 cut(s) 123
BslFI GGGAC 2 cut(s) 991, 1064
BslI CCNNNNNNNGG 2 cut(s) 41, 271
BsmAI GTCTC 1 cut(s) 892
BsmFI GGGAC 2 cut(s) 991, 1064
BsnI GGCC 4 cut(s) 45, 353, 854, 1096
Bso31I GGTCTC 1 cut(s) 892
Bsp120I GGGCCC 1 cut(s) 351
Bsp1286I GDGCHC 3 cut(s) 123, 355, 988
Bsp143I GATC 4 cut(s) 299, 334, 772, 1108
Bsp1720I GCTNAGC 1 cut(s) 817
BspACI CCGC 2 cut(s) 365, 1296
BspANI GGCC 4 cut(s) 45, 353, 854, 1096
BspCNI CTCAG 1 cut(s) 612
BspFNI CGCG 1 cut(s) 365
BspLI GGNNCC 3 cut(s) 27, 353, 987
BspMAI CTGCAG 2 cut(s) 409, 670
BspPI GGATC 1 cut(s) 307
BspT107I GGYRCC 2 cut(s) 25, 985
BspTNI GGTCTC 1 cut(s) 892
BsrDI GCAATG 2 cut(s) 153, 159
BsrI ACTGG 1 cut(s) 1275
BssECI CCNNGG 4 cut(s) 354, 556, 855, 856
BssMI GATC 4 cut(s) 299, 334, 772, 1108
Bst2UI CCWGG 2 cut(s) 356, 857
Bst4CI ACNGT 3 cut(s) 199, 789, 840
BstC8I GCNNGC 6 cut(s) 387, 522, 553, 666, 718, 1094
BstDEI CTNAG 2 cut(s) 599, 817
BstF5I GGATG 4 cut(s) 353, 435, 647, 1285
BstFNI CGCG 1 cut(s) 365
BstKTI GATC 4 cut(s) 302, 337, 775, 1111
BstMAI GTCTC 1 cut(s) 892
BstMBI GATC 4 cut(s) 299, 334, 772, 1108
BstMWI GCNNNNNNNGC 1 cut(s) 410
BstNI CCWGG 2 cut(s) 356, 857
BstNSI RCATGY 2 cut(s) 324, 555
BstSCI CCNGG 2 cut(s) 354, 855
BstSFI CTRYAG 3 cut(s) 405, 666, 792
BstSLI GKGCMC 2 cut(s) 355, 988
BstUI CGCG 1 cut(s) 365
BstV1I GCAGC 7 cut(s) 376, 391, 397, 419, 680, 1209, 1231
BstV2I GAAGAC 1 cut(s) 756
BstXI CCANNNNNNTGG 1 cut(s) 888
BsuRI GGCC 4 cut(s) 45, 353, 854, 1096
BtgZI GCGATG 2 cut(s) 995, 1003
BtsCI GGATG 4 cut(s) 353, 435, 647, 1285
BtsIMutI CAGTG 1 cut(s) 195
Cac8I GCNNGC 6 cut(s) 387, 522, 553, 666, 718, 1094
Cfr13I GGNCC 4 cut(s) 351, 352, 704, 853
Csp6I GTAC 2 cut(s) 26, 422
CviAII CATG 8 cut(s) 14, 47, 89, 321, 526, 552, 679, 1057
CviQI GTAC 2 cut(s) 26, 422
DdeI CTNAG 2 cut(s) 599, 817
DpnI GATC 4 cut(s) 301, 336, 774, 1110
DpnII GATC 4 cut(s) 299, 334, 772, 1108
Eco24I GRGCYC 1 cut(s) 355
Eco31I GGTCTC 1 cut(s) 892
Eco32I GATATC 1 cut(s) 1261
Eco47I GGWCC 1 cut(s) 704
Eco57I CTGAAG 1 cut(s) 261
EcoO109I RGGNCCY 1 cut(s) 352
EcoRI GAATTC 2 cut(s) 941, 1048
EcoRII CCWGG 2 cut(s) 354, 855
EcoRV GATATC 1 cut(s) 1261
EcoT38I GRGCYC 1 cut(s) 355
FaeI CATG 8 cut(s) 17, 50, 92, 324, 529, 555, 682, 1060
FaqI GGGAC 2 cut(s) 991, 1064
FatI CATG 8 cut(s) 13, 46, 88, 320, 525, 551, 678, 1056
FauNDI CATATG 1 cut(s) 129
FbaI TGATCA 3 cut(s) 334, 772, 1108
Fnu4HI GCNGC 7 cut(s) 390, 405, 408, 411, 669, 1220, 1223
FokI GGATG 4 cut(s) 360, 422, 634, 1292
FriOI GRGCYC 1 cut(s) 355
Fsp4HI GCNGC 7 cut(s) 390, 405, 408, 411, 669, 1220, 1223
FspBI CTAG 4 cut(s) 80, 734, 863, 1226
GluI GCNGC 7 cut(s) 390, 405, 408, 411, 669, 1220, 1223
GsaI CCCAGC 1 cut(s) 404
HaeIII GGCC 4 cut(s) 45, 353, 854, 1096
Hin1II CATG 8 cut(s) 17, 50, 92, 324, 529, 555, 682, 1060
HincII GTYRAC 1 cut(s) 237
HindII GTYRAC 1 cut(s) 237
HinfI GANTC 4 cut(s) 340, 682, 752, 1077
HphI GGTGA 2 cut(s) 501, 907
Hpy166II GTNNAC 2 cut(s) 237, 707
Hpy188I TCNGA 5 cut(s) 208, 339, 560, 602, 1138
Hpy188III TCNNGA 2 cut(s) 72, 1264
Hpy8I GTNNAC 2 cut(s) 237, 707
HpyAV CCTTC 6 cut(s) 367, 403, 467, 1055, 1141, 1286
HpyCH4III ACNGT 3 cut(s) 199, 789, 840
HpyCH4IV ACGT 1 cut(s) 123
HpyCH4V TGCA 7 cut(s) 133, 407, 413, 574, 642, 668, 743
HpyF10VI GCNNNNNNNGC 1 cut(s) 410
HpyF3I CTNAG 2 cut(s) 599, 817
HpySE526I ACGT 1 cut(s) 123
Hsp92II CATG 8 cut(s) 17, 50, 92, 324, 529, 555, 682, 1060
KpnI GGTACC 1 cut(s) 29
Ksp22I TGATCA 3 cut(s) 334, 772, 1108
Kzo9I GATC 4 cut(s) 299, 334, 772, 1108
LmnI GCTCC 3 cut(s) 277, 1019, 1216
Lsp1109I GCAGC 7 cut(s) 376, 391, 397, 419, 680, 1209, 1231
LweI GCATC 3 cut(s) 65, 444, 1293
MaeI CTAG 4 cut(s) 80, 734, 863, 1226
MaeII ACGT 1 cut(s) 123
MaeIII GTNAC 2 cut(s) 100, 633
MalI GATC 4 cut(s) 301, 336, 774, 1110
MboI GATC 4 cut(s) 299, 334, 772, 1108
MboII GAAGA 5 cut(s) 173, 221, 299, 382, 761
MhlI GDGCHC 3 cut(s) 123, 355, 988
MluCI AATT 8 cut(s) 51, 177, 246, 269, 848, 941, 1048, 1312
MlyI GAGTC 4 cut(s) 334, 676, 746, 1086
MmeI TCCRAC 1 cut(s) 1025
MnlI CCTC 7 cut(s) 21, 151, 246, 268, 566, 990, 999
MroXI GAANNNNTTC 1 cut(s) 941
MseI TTAA 6 cut(s) 294, 381, 431, 501, 828, 1119
MslI CAYNNNNRTG 1 cut(s) 886
MspA1I CMGCKG 3 cut(s) 404, 410, 1222
MspR9I CCNGG 2 cut(s) 356, 857
MvaI CCWGG 2 cut(s) 356, 857
MvnI CGCG 1 cut(s) 365
MwoI GCNNNNNNNGC 1 cut(s) 410
NdeI CATATG 1 cut(s) 129
NdeII GATC 4 cut(s) 299, 334, 772, 1108
NlaIII CATG 8 cut(s) 17, 50, 92, 324, 529, 555, 682, 1060
NlaIV GGNNCC 3 cut(s) 27, 353, 987
NspI RCATGY 2 cut(s) 324, 555
PaeI GCATGC 1 cut(s) 555
PasI CCCWGGG 1 cut(s) 856
PciI ACATGT 1 cut(s) 320
PcsI WCGNNNNNNNCGW 1 cut(s) 114
PdmI GAANNNNTTC 1 cut(s) 941
PkrI GCNGC 7 cut(s) 391, 406, 409, 412, 670, 1221, 1224
PleI GAGTC 4 cut(s) 334, 676, 746, 1085
PpsI GAGTC 4 cut(s) 334, 676, 746, 1085
PscI ACATGT 1 cut(s) 320
PshBI ATTAAT 1 cut(s) 294
Psp6I CCWGG 2 cut(s) 354, 855
PspFI CCCAGC 1 cut(s) 400
PspGI CCWGG 2 cut(s) 354, 855
PspN4I GGNNCC 3 cut(s) 27, 353, 987
PspOMI GGGCCC 1 cut(s) 351
PspPI GGNCC 4 cut(s) 351, 352, 704, 853
PstI CTGCAG 2 cut(s) 409, 670
PvuII CAGCTG 3 cut(s) 404, 410, 1222
RsaI GTAC 2 cut(s) 27, 423
RsaNI GTAC 2 cut(s) 26, 422
RseI CAYNNNNRTG 1 cut(s) 886
SaqAI TTAA 6 cut(s) 294, 381, 431, 501, 828, 1119
SatI GCNGC 7 cut(s) 390, 405, 408, 411, 669, 1220, 1223
Sau3AI GATC 4 cut(s) 299, 334, 772, 1108
Sau96I GGNCC 4 cut(s) 351, 352, 704, 853
SchI GAGTC 4 cut(s) 334, 676, 746, 1086
ScrFI CCNGG 2 cut(s) 356, 857
SduI GDGCHC 3 cut(s) 123, 355, 988
SfaNI GCATC 3 cut(s) 65, 444, 1293
SfcI CTRYAG 3 cut(s) 405, 666, 792
SinI GGWCC 1 cut(s) 704
SmiMI CAYNNNNRTG 1 cut(s) 886
SmlI CTYRAG 3 cut(s) 62, 659, 963
SmoI CTYRAG 3 cut(s) 62, 659, 963
SphI GCATGC 1 cut(s) 555
Sse9I AATT 8 cut(s) 51, 177, 246, 269, 848, 941, 1048, 1312
SsiI CCGC 2 cut(s) 365, 1296
SspMI CTAG 4 cut(s) 80, 734, 863, 1226
StyD4I CCNGG 2 cut(s) 354, 855
TaaI ACNGT 3 cut(s) 199, 789, 840
TaiI ACGT 1 cut(s) 126
TaqI TCGA 3 cut(s) 465, 1123, 1257
TasI AATT 8 cut(s) 51, 177, 246, 269, 848, 941, 1048, 1312
Tru1I TTAA 6 cut(s) 294, 381, 431, 501, 828, 1119
Tru9I TTAA 6 cut(s) 294, 381, 431, 501, 828, 1119
TscAI CASTG 1 cut(s) 202
TseI GCWGC 7 cut(s) 389, 404, 407, 410, 668, 1219, 1222
TspDTI ATGAA 7 cut(s) 116, 152, 170, 486, 542, 939, 1188
TspRI CASTG 1 cut(s) 202
VpaK11BI GGWCC 1 cut(s) 704
VspI ATTAAT 1 cut(s) 294
XapI RAATTY 4 cut(s) 177, 941, 1048, 1312
XceI RCATGY 2 cut(s) 324, 555
XmnI GAANNNNTTC 1 cut(s) 941
XspI CTAG 4 cut(s) 80, 734, 863, 1226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.