pycom11g03190

Helitron helicase-like domain at N-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0001547
Physical Location & Seq
Forward (+)
2444188 .. 2446000
1813 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1689 bp
ATGTTTTCATTTACTTCTATGGGAGCAAAAGTTGATAACTCAATTAATGACGGATCAAGTCCTTACATTTTTAAAATTAGTGGTCAAGTTTGTCATTTAATGGGATCTCTTTTACCTGTTGGCAATGAGTCTCCAAAATTTGCTCAATTATATGTTTATGATACATATAATGAAGTTCAAAATCGTCTCAAAGCTTTTAATTTTGATGGAAAAAATGGAAGGCTTGACGAAAGAATTGTTCAAGAATTGATTAAAATGTTTGATGAATTGAATGAGTTAGTCAAACTTTTTCGAACAGCAAGAGATAAATTTAAAACAAATGGTCTTATGAATTTAAGAATGACTTTATTAGGTAGGCAACCAAATGATGGGAAACAATATGATGAACCAACAAGTGATGAGATAGGTGGTTTAATAGTTGGTGATATAGGTTTGTCTGACTCTAATAGAGACATCATTATAGAATGTAAAAGCGAGGGATTAAAGCGTGTTACAAAATTGAATCTAAAATTTATGGCCCTTCAATATCCTATTCTTTTTCCGTATGGTGAAGACGGGTATAGGCCGGATTTAAAGTGGAATCAAAATTATAAAAGGCCAAAAACAAAAAGACAAAGAGTGCCTATGAGAGCTTATATAGCTTATCAAATTCAGGAAAGAGAACCATGTTTAACTACGTTGTTAAAAGGAGGTAGACTTTTTCAACAATATTTGGTTGATTCGTATGCAACAGTTGAGGAGGACAGGTTAGATTACATTAGACAAAATCAAAAAAATTTGAGGAGTGAAATTTACAAGGGGATTTATGATGCAATATCAAGAGGTGATAATGATGCAAACAATTTAGAACAAAAAATTGTGTTGCCTGCTTCGTATACAGGAAGTCCTAGATATATGATCAATAATTATCAAGATGCTATGGCTATTTGTAGAGAGTATGGTCATCCTGATTTGTTTATAACATTTACCTGTAATGTAAAATGGCCAGAAATTATAAGAGAGTTTGAAAAAAAACAGGGTTACAAACCTGAAGATAGACCTGATATAATTTCTAGAATATTTAAAATGAAGCTTGATGATCTGCTTGATTTCATCAAATCTGGACAGCCTTTTGGAGAGGTTGATGCAAGTAAGTTCTACAATGAATTTCAAAAAAGAGGTTTGCCTCATTCTCATATGTTAATATGGTTGAGACCAAAATTTAAATGCTACACAGCATCAGATATAGATTCTATCATTTCAGCTGAGTTACCAGACAAATTTATGAACCTTCGGCTTTATGAAATTGTTAATCAATTTATGATTCATGGTCCACGTGGTTATATCAATTGGAATTCTTCTTGCATGCGAGAAAATAAATGCACCAAAAATTTTCCCAAGGCGTATAAAAATGAGACGGTTTTTGACCCGAGTGGCTTTCCTGTTTATAGGCGTCGTGATGATGAGACAAAATTTGTAGTAAAAAATGGAATTAAAGTTGATAATAGTTTTGTTGTTCCTTATAATTCTGAATTGCTTTTGAGATATAATGCTCATATAAATGTTGAATTATGTTGTCAATCAACACTTATAAAATATTTGTTCAAATATGTAAATAAAGGTTCAGATAGAGCACGGTTGGTTCTTCAGCAAAATCCAAATGATGAAATATTAACATATCTAAATTGTAGATATATATCACCTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N

Protein Analysis

563

Amino Acids

65.65

Weight (kDa)

8.87

Isoelectric Point (pI)

37.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helitron_like_N PF14214 212 - 395 4.6e-54 Helitron helicase-like domain at N-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 5 cut(s) 591, 959, 995, 1503, 1571
AccB7I CCANNNNNTGG 1 cut(s) 368
AccI GTMKAC 2 cut(s) 694, 875
AclWI GGATC 2 cut(s) 61, 112
AcoI YGGCCR 1 cut(s) 983
AcuI CTGAAG 2 cut(s) 1050, 1610
AcvI CACGTG 1 cut(s) 1316
AcyI GRCGYC 1 cut(s) 1432
AfiI CCNNNNNNNGG 1 cut(s) 368
AluBI AGCT 5 cut(s) 194, 632, 641, 1072, 1244
AluI AGCT 5 cut(s) 194, 632, 641, 1072, 1244
Alw21I GWGCWC 1 cut(s) 1615
Alw26I GTCTC 6 cut(s) 135, 191, 444, 1186, 1388, 1439
AlwI GGATC 2 cut(s) 61, 112
Ama87I CYCGRG 1 cut(s) 1408
AoxI GGCC 4 cut(s) 516, 563, 596, 983
AseI ATTAAT 1 cut(s) 45
AspS9I GGNCC 2 cut(s) 517, 1310
AsuHPI GGTGA 4 cut(s) 434, 560, 836, 1671
AsuII TTCGAA 1 cut(s) 292
AvaI CYCGRG 1 cut(s) 1408
AvaII GGWCC 1 cut(s) 1310
BalI TGGCCA 1 cut(s) 985
BbrPI CACGTG 1 cut(s) 1316
BbsI GAAGAC 1 cut(s) 558
Bbv12I GWGCWC 1 cut(s) 1615
BccI CCATC 2 cut(s) 200, 362
BclI TGATCA 1 cut(s) 897
BcoDI GTCTC 6 cut(s) 135, 191, 444, 1186, 1388, 1439
BfaI CTAG 2 cut(s) 888, 1053
Bme18I GGWCC 1 cut(s) 1310
BmeT110I CYCGRG 1 cut(s) 1408
BmgT120I GGNCC 2 cut(s) 517, 1310
BmsI GCATC 5 cut(s) 799, 823, 904, 1114, 1226
BpiI GAAGAC 1 cut(s) 558
Bpu14I TTCGAA 1 cut(s) 292
BsaAI YACGTR 1 cut(s) 1316
BsaBI GATNNNNATC 1 cut(s) 1675
BsaHI GRCGYC 1 cut(s) 1432
BsaI GGTCTC 1 cut(s) 1186
BsaJI CCNNGG 1 cut(s) 1377
BsaXI ACNNNNNCTCC 4 cut(s) 15, 45, 731, 761
Bsc4I CCNNNNNNNGG 1 cut(s) 368
Bse3DI GCAATG 1 cut(s) 130
Bse8I GATNNNNATC 1 cut(s) 1675
BseDI CCNNGG 1 cut(s) 1377
BseGI GGATG 1 cut(s) 943
BseJI GATNNNNATC 1 cut(s) 1675
BseLI CCNNNNNNNGG 1 cut(s) 368
BseMI GCAATG 1 cut(s) 130
BseMII CTCAG 1 cut(s) 1236
BseRI GAGGAG 2 cut(s) 752, 796
BshFI GGCC 4 cut(s) 518, 565, 598, 985
BsiHKAI GWGCWC 1 cut(s) 1615
BsiHKCI CYCGRG 1 cut(s) 1408
BsiSI CCGG 1 cut(s) 566
BslI CCNNNNNNNGG 1 cut(s) 368
BsmAI GTCTC 6 cut(s) 135, 191, 444, 1186, 1388, 1439
BsmBI CGTCTC 2 cut(s) 191, 1388
BsnI GGCC 4 cut(s) 518, 565, 598, 985
Bso31I GGTCTC 1 cut(s) 1186
BsoBI CYCGRG 1 cut(s) 1408
Bsp119I TTCGAA 1 cut(s) 292
Bsp1286I GDGCHC 1 cut(s) 1615
Bsp143I GATC 4 cut(s) 53, 104, 897, 1078
BspANI GGCC 4 cut(s) 518, 565, 598, 985
BspCNI CTCAG 1 cut(s) 1237
BspPI GGATC 2 cut(s) 61, 112
BspT104I TTCGAA 1 cut(s) 292
BspTNI GGTCTC 1 cut(s) 1186
BsrDI GCAATG 1 cut(s) 130
BssECI CCNNGG 1 cut(s) 1377
BssMI GATC 4 cut(s) 53, 104, 897, 1078
BssNAI GTATAC 1 cut(s) 876
BssNI GRCGYC 1 cut(s) 1432
BssT1I CCWWGG 1 cut(s) 1377
Bst1107I GTATAC 1 cut(s) 876
Bst4CI ACNGT 3 cut(s) 733, 1399, 1617
BstACI GRCGYC 1 cut(s) 1432
BstBAI YACGTR 1 cut(s) 1316
BstBI TTCGAA 1 cut(s) 292
BstC8I GCNNGC 2 cut(s) 867, 1346
BstDEI CTNAG 1 cut(s) 1245
BstF5I GGATG 1 cut(s) 943
BstKTI GATC 4 cut(s) 56, 107, 900, 1081
BstMAI GTCTC 6 cut(s) 135, 191, 444, 1186, 1388, 1439
BstMBI GATC 4 cut(s) 53, 104, 897, 1078
BstMWI GCNNNNNNNGC 1 cut(s) 638
BstNSI RCATGY 1 cut(s) 1348
BstV2I GAAGAC 1 cut(s) 558
BstX2I RGATCY 1 cut(s) 104
BstYI RGATCY 1 cut(s) 104
BstZ17I GTATAC 1 cut(s) 876
BsuRI GGCC 4 cut(s) 518, 565, 598, 985
BtsCI GGATG 1 cut(s) 943
Cac8I GCNNGC 2 cut(s) 867, 1346
Cfr13I GGNCC 2 cut(s) 517, 1310
CseI GACGC 1 cut(s) 1421
CviAII CATG 3 cut(s) 666, 1307, 1345
DdeI CTNAG 1 cut(s) 1245
DpnI GATC 4 cut(s) 55, 106, 899, 1080
DpnII GATC 4 cut(s) 53, 104, 897, 1078
DraI TTTAAA 5 cut(s) 73, 313, 573, 1063, 1204
EaeI YGGCCR 1 cut(s) 983
Eco130I CCWWGG 1 cut(s) 1377
Eco31I GGTCTC 1 cut(s) 1186
Eco47I GGWCC 1 cut(s) 1310
Eco57I CTGAAG 2 cut(s) 1050, 1610
Eco72I CACGTG 1 cut(s) 1316
Eco88I CYCGRG 1 cut(s) 1408
EcoRI GAATTC 1 cut(s) 1333
EcoT14I CCWWGG 1 cut(s) 1377
ErhI CCWWGG 1 cut(s) 1377
Esp3I CGTCTC 2 cut(s) 191, 1388
FaeI CATG 3 cut(s) 669, 1310, 1348
FalI AAGNNNNNCTT 2 cut(s) 328, 360
FatI CATG 3 cut(s) 665, 1306, 1344
FauNDI CATATG 1 cut(s) 1176
FbaI TGATCA 1 cut(s) 897
FblI GTMKAC 2 cut(s) 694, 875
FokI GGATG 1 cut(s) 930
FspBI CTAG 2 cut(s) 888, 1053
HaeIII GGCC 4 cut(s) 518, 565, 598, 985
HapII CCGG 1 cut(s) 566
HgaI GACGC 1 cut(s) 1421
Hin1I GRCGYC 1 cut(s) 1432
Hin1II CATG 3 cut(s) 669, 1310, 1348
HindIII AAGCTT 2 cut(s) 192, 1070
HinfI GANTC 7 cut(s) 128, 440, 502, 580, 719, 1229, 1303
HpaII CCGG 1 cut(s) 566
HphI GGTGA 4 cut(s) 434, 560, 836, 1671
Hpy166II GTNNAC 3 cut(s) 695, 876, 1313
Hpy188I TCNGA 4 cut(s) 439, 1222, 1510, 1606
Hpy188III TCNNGA 8 cut(s) 242, 653, 819, 911, 947, 1053, 1101, 1436
Hpy8I GTNNAC 3 cut(s) 695, 876, 1313
Hpy99I CGWCG 1 cut(s) 1437
HpyAV CCTTC 3 cut(s) 213, 530, 1280
HpyCH4III ACNGT 3 cut(s) 733, 1399, 1617
HpyCH4IV ACGT 2 cut(s) 677, 1315
HpyCH4V TGCA 6 cut(s) 728, 812, 836, 1127, 1344, 1362
HpyF10VI GCNNNNNNNGC 1 cut(s) 638
HpyF3I CTNAG 1 cut(s) 1245
HpySE526I ACGT 2 cut(s) 677, 1315
Hsp92I GRCGYC 1 cut(s) 1432
Hsp92II CATG 3 cut(s) 669, 1310, 1348
Ksp22I TGATCA 1 cut(s) 897
Kzo9I GATC 4 cut(s) 53, 104, 897, 1078
LmnI GCTCC 1 cut(s) 23
LweI GCATC 5 cut(s) 799, 823, 904, 1114, 1226
MaeI CTAG 2 cut(s) 888, 1053
MaeII ACGT 2 cut(s) 677, 1315
MaeIII GTNAC 3 cut(s) 490, 1019, 1248
MalI GATC 4 cut(s) 55, 106, 899, 1080
MboI GATC 4 cut(s) 53, 104, 897, 1078
MboII GAAGA 4 cut(s) 563, 1043, 1329, 1616
MfeI CAATTG 1 cut(s) 1327
MflI RGATCY 1 cut(s) 104
MhlI GDGCHC 1 cut(s) 1615
MlsI TGGCCA 1 cut(s) 985
MluNI TGGCCA 1 cut(s) 985
MlyI GAGTC 2 cut(s) 137, 434
MnlI CCTC 9 cut(s) 469, 683, 730, 733, 774, 815, 1111, 1151, 1176
Mox20I TGGCCA 1 cut(s) 985
MscI TGGCCA 1 cut(s) 985
MslI CAYNNNNRTG 1 cut(s) 1539
Msp20I TGGCCA 1 cut(s) 985
MspA1I CMGCKG 1 cut(s) 1244
MspI CCGG 1 cut(s) 566
MunI CAATTG 1 cut(s) 1327
MwoI GCNNNNNNNGC 1 cut(s) 638
NdeI CATATG 1 cut(s) 1176
NdeII GATC 4 cut(s) 53, 104, 897, 1078
NlaIII CATG 3 cut(s) 669, 1310, 1348
NspI RCATGY 1 cut(s) 1348
NspV TTCGAA 1 cut(s) 292
PaeI GCATGC 1 cut(s) 1348
PfeI GAWTC 5 cut(s) 502, 580, 719, 1229, 1303
PflMI CCANNNNNTGG 1 cut(s) 368
PleI GAGTC 2 cut(s) 136, 434
PmaCI CACGTG 1 cut(s) 1316
PmlI CACGTG 1 cut(s) 1316
PpsI GAGTC 2 cut(s) 136, 434
Ppu21I YACGTR 1 cut(s) 1316
PshBI ATTAAT 1 cut(s) 45
PsiI TTATAA 5 cut(s) 591, 959, 995, 1503, 1571
PspCI CACGTG 1 cut(s) 1316
PspPI GGNCC 2 cut(s) 517, 1310
PsuI RGATCY 1 cut(s) 104
PvuII CAGCTG 1 cut(s) 1244
RseI CAYNNNNRTG 1 cut(s) 1539
Sau3AI GATC 4 cut(s) 53, 104, 897, 1078
Sau96I GGNCC 2 cut(s) 517, 1310
SchI GAGTC 2 cut(s) 137, 434
SduI GDGCHC 1 cut(s) 1615
SfaNI GCATC 5 cut(s) 799, 823, 904, 1114, 1226
SfuI TTCGAA 1 cut(s) 292
SinI GGWCC 1 cut(s) 1310
SmiI ATTTAAAT 1 cut(s) 1204
SmiMI CAYNNNNRTG 1 cut(s) 1539
SphI GCATGC 1 cut(s) 1348
SspI AATATT 4 cut(s) 710, 1059, 1577, 1650
SspMI CTAG 2 cut(s) 888, 1053
StyI CCWWGG 1 cut(s) 1377
SwaI ATTTAAAT 1 cut(s) 1204
TaaI ACNGT 3 cut(s) 733, 1399, 1617
TaiI ACGT 2 cut(s) 680, 1318
TaqI TCGA 1 cut(s) 292
TfiI GAWTC 5 cut(s) 502, 580, 719, 1229, 1303
TspGWI ACGGA 2 cut(s) 66, 531
Van91I CCANNNNNTGG 1 cut(s) 368
VpaK11BI GGWCC 1 cut(s) 1310
VspI ATTAAT 1 cut(s) 45
XbaI TCTAGA 1 cut(s) 1052
XceI RCATGY 1 cut(s) 1348
XmiI GTMKAC 2 cut(s) 694, 875
XspI CTAG 2 cut(s) 888, 1053
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.