pycom12g19280

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0001788
Physical Location & Seq
Forward (+)
21182154 .. 21183283
1130 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 654 bp
ATGCAGTTTCTAATTATATCTTCAACATTGGCTCTCTTCTTTTTATTGAGTGAAGTGGATGCAAGGTATGTCCCAGGGATCTATAATCTTCAAGACAATGTTATAAGTACCATCATAGTCGATCCGAATGGCCTAGGAAATTTTACCTCGGTGCAACAAGCCACTGATTCCGTAGCACCAAATAACACTGCGTGGACTCCAATTCACATCAACTATGGTATCTACACCTACTTTTATGTATGTAGTGAAAAAGTTCAGATCCCAGTAGACAAACCATACATCTATATTGAAGGAGACTCTGATCACCAACCTGTGATCCAATATGCGGACGGTGGAAGTATTCTCACTAGTCCTACATTTAAAGCACTTGCAGATAATTTTGTGGCACGGAATATAATCTTCAAGGTTAAATATCAAACATACGACAATATTATTGCTGACCTATATGATGCCAATGGAAAAAGGAAGACCACTACATGGGCAGTTGCCCTAGATGTATCTGGATATAAAGCAAGTTTTTACCACTGTAGCTTTTCTAGTTTGCAAGACACATTGAGTGATGCCAAAGGTCGTCACTATTTCTATGATTGCACCATTGAAGGACTTATAGACTTCGTTTGGGGCCATGGCCAATCAATTTATGAGGCTCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

218

Amino Acids

24.4

Weight (kDa)

4.9

Isoelectric Point (pI)

38.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 38 - 214 2e-20 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 104
AccB7I CCANNNNNTGG 1 cut(s) 477
AccI GTMKAC 1 cut(s) 267
AciI CCGC 1 cut(s) 326
AclWI GGATC 4 cut(s) 86, 116, 253, 310
AcoI YGGCCR 1 cut(s) 628
AcsI RAATTY 1 cut(s) 139
AdeI CACNNNGTG 1 cut(s) 192
AfaI GTAC 1 cut(s) 109
AfiI CCNNNNNNNGG 2 cut(s) 325, 477
AgsI TTSAA 5 cut(s) 24, 92, 290, 403, 599
AhlI ACTAGT 1 cut(s) 347
AjnI CCWGG 1 cut(s) 73
AluBI AGCT 1 cut(s) 531
AluI AGCT 1 cut(s) 531
Alw26I GTCTC 1 cut(s) 288
AlwI GGATC 4 cut(s) 86, 116, 253, 310
AoxI GGCC 3 cut(s) 130, 622, 628
ApoI RAATTY 1 cut(s) 139
Asp700I GAANNNNTTC 1 cut(s) 252
AspA2I CCTAGG 1 cut(s) 133
AspS9I GGNCC 1 cut(s) 622
AsuHPI GGTGA 1 cut(s) 296
AvrII CCTAGG 1 cut(s) 133
BaeI ACNNNNGTAYC 2 cut(s) 202, 235
BalI TGGCCA 1 cut(s) 630
BbsI GAAGAC 1 cut(s) 473
BccI CCATC 1 cut(s) 119
BciT130I CCWGG 1 cut(s) 75
BclI TGATCA 1 cut(s) 301
BcoDI GTCTC 1 cut(s) 288
BcuI ACTAGT 1 cut(s) 347
BfaI CTAG 4 cut(s) 134, 348, 491, 537
BfmI CTRYAG 1 cut(s) 526
BlnI CCTAGG 1 cut(s) 133
Bme1390I CCNGG 1 cut(s) 75
BmgT120I GGNCC 1 cut(s) 622
BmiI GGNNCC 1 cut(s) 623
BmrFI CCNGG 1 cut(s) 75
BmrI ACTGGG 1 cut(s) 257
BmsI GCATC 3 cut(s) 49, 439, 550
BmuI ACTGGG 1 cut(s) 257
BpiI GAAGAC 1 cut(s) 473
BsaJI CCNNGG 5 cut(s) 73, 74, 133, 147, 625
Bsc4I CCNNNNNNNGG 2 cut(s) 325, 477
Bse1I ACTGG 1 cut(s) 263
BseBI CCWGG 1 cut(s) 75
BseDI CCNNGG 5 cut(s) 73, 74, 133, 147, 625
BseGI GGATG 1 cut(s) 64
BseLI CCNNNNNNNGG 2 cut(s) 325, 477
BseNI ACTGG 1 cut(s) 263
BshFI GGCC 3 cut(s) 132, 624, 630
BslFI GGGAC 1 cut(s) 56
BslI CCNNNNNNNGG 2 cut(s) 325, 477
BsmAI GTCTC 1 cut(s) 288
BsmFI GGGAC 1 cut(s) 56
BsnI GGCC 3 cut(s) 132, 624, 630
Bsp143I GATC 5 cut(s) 78, 121, 258, 301, 315
Bsp19I CCATGG 1 cut(s) 625
BspACI CCGC 1 cut(s) 326
BspANI GGCC 3 cut(s) 132, 624, 630
BspLI GGNNCC 1 cut(s) 623
BspPI GGATC 4 cut(s) 86, 116, 253, 310
BsrI ACTGG 1 cut(s) 263
BssECI CCNNGG 5 cut(s) 73, 74, 133, 147, 625
BssMI GATC 5 cut(s) 78, 121, 258, 301, 315
BssT1I CCWWGG 2 cut(s) 133, 625
Bst2UI CCWGG 1 cut(s) 75
Bst4CI ACNGT 2 cut(s) 332, 527
Bst6I CTCTTC 1 cut(s) 41
BstDSI CCRYGG 1 cut(s) 625
BstF5I GGATG 1 cut(s) 64
BstKTI GATC 5 cut(s) 81, 124, 261, 304, 318
BstMAI GTCTC 1 cut(s) 288
BstMBI GATC 5 cut(s) 78, 121, 258, 301, 315
BstNI CCWGG 1 cut(s) 75
BstSCI CCNGG 1 cut(s) 73
BstSFI CTRYAG 1 cut(s) 526
BstV2I GAAGAC 1 cut(s) 473
BstX2I RGATCY 2 cut(s) 78, 258
BstYI RGATCY 2 cut(s) 78, 258
BsuRI GGCC 3 cut(s) 132, 624, 630
BtgI CCRYGG 1 cut(s) 625
BtsCI GGATG 1 cut(s) 64
BtsI GCAGTG 1 cut(s) 186
BtsIMutI CAGTG 3 cut(s) 162, 186, 523
Cfr13I GGNCC 1 cut(s) 622
Csp6I GTAC 1 cut(s) 108
CviAII CATG 2 cut(s) 477, 626
CviJI RGCY 7 cut(s) 32, 132, 161, 531, 624, 630, 647
CviKI_1 RGCY 7 cut(s) 32, 132, 161, 531, 624, 630, 647
CviQI GTAC 1 cut(s) 108
DpnI GATC 5 cut(s) 80, 123, 260, 303, 317
DpnII GATC 5 cut(s) 78, 121, 258, 301, 315
DraI TTTAAA 1 cut(s) 361
DraIII CACNNNGTG 1 cut(s) 192
EaeI YGGCCR 1 cut(s) 628
Eam1104I CTCTTC 1 cut(s) 41
EarI CTCTTC 1 cut(s) 41
Eco130I CCWWGG 2 cut(s) 133, 625
EcoRII CCWGG 1 cut(s) 73
EcoT14I CCWWGG 2 cut(s) 133, 625
ErhI CCWWGG 2 cut(s) 133, 625
FaeI CATG 2 cut(s) 480, 629
FaqI GGGAC 1 cut(s) 56
FatI CATG 2 cut(s) 476, 625
FbaI TGATCA 1 cut(s) 301
FblI GTMKAC 1 cut(s) 267
FokI GGATG 1 cut(s) 71
FspBI CTAG 4 cut(s) 134, 348, 491, 537
HaeIII GGCC 3 cut(s) 132, 624, 630
Hin1II CATG 2 cut(s) 480, 629
HinfI GANTC 3 cut(s) 167, 196, 296
HphI GGTGA 1 cut(s) 296
Hpy166II GTNNAC 2 cut(s) 195, 268
Hpy188I TCNGA 3 cut(s) 126, 258, 301
Hpy188III TCNNGA 2 cut(s) 92, 501
Hpy8I GTNNAC 2 cut(s) 195, 268
HpyAV CCTTC 2 cut(s) 284, 593
HpyCH4III ACNGT 2 cut(s) 332, 527
HpyCH4V TGCA 6 cut(s) 4, 62, 154, 371, 544, 591
Hsp92II CATG 2 cut(s) 480, 629
Ksp22I TGATCA 1 cut(s) 301
Kzo9I GATC 5 cut(s) 78, 121, 258, 301, 315
LpnPI CCDG 5 cut(s) 60, 87, 276, 324, 486
LweI GCATC 3 cut(s) 49, 439, 550
MaeI CTAG 4 cut(s) 134, 348, 491, 537
MaeIII GTNAC 1 cut(s) 572
MalI GATC 5 cut(s) 80, 123, 260, 303, 317
MboI GATC 5 cut(s) 78, 121, 258, 301, 315
MboII GAAGA 5 cut(s) 12, 28, 80, 391, 478
MflI RGATCY 2 cut(s) 78, 258
MlsI TGGCCA 1 cut(s) 630
MluCI AATT 5 cut(s) 12, 139, 201, 376, 636
MluNI TGGCCA 1 cut(s) 630
MlyI GAGTC 2 cut(s) 190, 290
MnlI CCTC 2 cut(s) 157, 637
Mox20I TGGCCA 1 cut(s) 630
MroXI GAANNNNTTC 1 cut(s) 252
MscI TGGCCA 1 cut(s) 630
MseI TTAA 2 cut(s) 360, 408
Msp20I TGGCCA 1 cut(s) 630
MspR9I CCNGG 1 cut(s) 75
MvaI CCWGG 1 cut(s) 75
NcoI CCATGG 1 cut(s) 625
NdeII GATC 5 cut(s) 78, 121, 258, 301, 315
NlaIII CATG 2 cut(s) 480, 629
NlaIV GGNNCC 1 cut(s) 623
NmuCI GTSAC 1 cut(s) 572
PasI CCCWGGG 1 cut(s) 74
PdmI GAANNNNTTC 1 cut(s) 252
PfeI GAWTC 1 cut(s) 167
PflMI CCANNNNNTGG 1 cut(s) 477
PleI GAGTC 2 cut(s) 190, 290
PpsI GAGTC 2 cut(s) 190, 290
PsiI TTATAA 1 cut(s) 104
Psp6I CCWGG 1 cut(s) 73
PspGI CCWGG 1 cut(s) 73
PspN4I GGNNCC 1 cut(s) 623
PspPI GGNCC 1 cut(s) 622
PsuI RGATCY 2 cut(s) 78, 258
RsaI GTAC 1 cut(s) 109
RsaNI GTAC 1 cut(s) 108
SaqAI TTAA 2 cut(s) 360, 408
Sau3AI GATC 5 cut(s) 78, 121, 258, 301, 315
Sau96I GGNCC 1 cut(s) 622
SchI GAGTC 2 cut(s) 190, 290
ScrFI CCNGG 1 cut(s) 75
SetI ASST 8 cut(s) 68, 149, 230, 313, 408, 444, 533, 571
SfaNI GCATC 3 cut(s) 49, 439, 550
SfcI CTRYAG 1 cut(s) 526
SpeI ACTAGT 1 cut(s) 347
Sse9I AATT 5 cut(s) 12, 139, 201, 376, 636
SsiI CCGC 1 cut(s) 326
SspI AATATT 1 cut(s) 430
SspMI CTAG 4 cut(s) 134, 348, 491, 537
StyD4I CCNGG 1 cut(s) 73
StyI CCWWGG 2 cut(s) 133, 625
TaaI ACNGT 2 cut(s) 332, 527
TaqI TCGA 1 cut(s) 120
TasI AATT 5 cut(s) 12, 139, 201, 376, 636
TfiI GAWTC 1 cut(s) 167
Tru1I TTAA 2 cut(s) 360, 408
Tru9I TTAA 2 cut(s) 360, 408
TscAI CASTG 3 cut(s) 169, 193, 530
TseFI GTSAC 1 cut(s) 572
Tsp45I GTSAC 1 cut(s) 572
TspGWI ACGGA 2 cut(s) 160, 403
TspRI CASTG 3 cut(s) 169, 193, 530
Van91I CCANNNNNTGG 1 cut(s) 477
XapI RAATTY 1 cut(s) 139
XmaJI CCTAGG 1 cut(s) 133
XmiI GTMKAC 1 cut(s) 267
XmnI GAANNNNTTC 1 cut(s) 252
XspI CTAG 4 cut(s) 134, 348, 491, 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.