pycom17g07520

WSTF, HB1, Itc1p, MBD9 motif 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0000989
Physical Location & Seq
Reverse (-)
5481220 .. 5482624
1405 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 816 bp
ATGAAGGAGCTGCCTACAGAGTGTTTTATTGAAGGCGGTGAGGGATACGATGCCTTAAACTTCTCCCAAAAGCTTAGGCTTCTGATTTTCCTTTGTGATGAGGCTCTTAACACAAAGACGCTGAGGGGATGGATTGAGGAGCAAAATGAAAGATCTGTCCAACATAAAAGGGACACGAAAGCAAAAGTTGGTCTGGCAAAGGATAAGGAGAGAGATCTCCAAGCAAAGTTGCAAGAGCAGATGGCTAAGAGGACCACTGCAGAGAATAGCGATGCTGTCCCGATTTCAGAGTCGAGCATCCTGACTTCAGAGTCGGACTCCATTGCTTCAGTACTCAGAAGTGAGTTAGCTCAAGCTCACATGGAGGTGGTCGAAGCAATGGCCATGGTACCCGAAAGCATTGAAATATGTGAGGCTGTTAGAATAGAGCCCTACATTGTGGATGCTGATGGCCATGCCTTCTGGAAACTGAGAGGCCATTTAGATGAAGATATATTGCTTCAAGATCTGGGATCCTGGGATGGAACTCCATCTGGTGAACAATGGTTCGTCTGCGGTGCTGAAATGAAAGAGCCAATTGAAAAATACCGCTCTTCTCTGGCTCTAAAGGAAAAACAGGCCAAGAGGGAGCAGAGGCGTTACAGAATTTCTTCAAGCAATGATGAAAAATTGCAGCTTTCCCAAACTTCTTCGGAAAGCAATGAAGAAAACGCGCAGGGTTCCCAGACTCCTGATAGCAATGGCGAAAGCGTGCAGGCATCCCGAGCTCCAGAATGCAATGAAGAGAACATGGAGCTCGGTCCTTCGGCGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDT WHIM1 zf-4CXXC_R1

Protein Analysis

272

Amino Acids

30.37

Weight (kDa)

4.72

Isoelectric Point (pI)

67.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 310
Acc65I GGTACC 1 cut(s) 388
AccB1I GGYRCC 1 cut(s) 388
AccBSI CCGCTC 1 cut(s) 591
AccII CGCG 1 cut(s) 713
AciI CCGC 3 cut(s) 36, 555, 589
AclWI GGATC 2 cut(s) 507, 520
AcoI YGGCCR 2 cut(s) 381, 451
AcsI RAATTY 1 cut(s) 645
AcuI CTGAAG 2 cut(s) 291, 312
AfaI GTAC 2 cut(s) 333, 390
AgsI TTSAA 5 cut(s) 32, 404, 503, 581, 654
AjnI CCWGG 1 cut(s) 515
AluBI AGCT 7 cut(s) 10, 73, 350, 356, 676, 767, 796
AluI AGCT 7 cut(s) 10, 73, 350, 356, 676, 767, 796
Alw21I GWGCWC 2 cut(s) 769, 798
AlwI GGATC 2 cut(s) 507, 520
Ama87I CYCGRG 1 cut(s) 762
AoxI GGCC 4 cut(s) 381, 451, 475, 618
ApeKI GCWGC 2 cut(s) 10, 673
ApoI RAATTY 1 cut(s) 645
Asp700I GAANNNNTTC 1 cut(s) 649
Asp718I GGTACC 1 cut(s) 388
AspLEI GCGC 1 cut(s) 715
AspS9I GGNCC 2 cut(s) 252, 800
AsuHPI GGTGA 2 cut(s) 50, 548
AvaI CYCGRG 1 cut(s) 762
AvaII GGWCC 2 cut(s) 252, 800
BalI TGGCCA 2 cut(s) 383, 453
BamHI GGATCC 1 cut(s) 512
BanI GGYRCC 1 cut(s) 388
BanII GRGCYC 3 cut(s) 432, 769, 798
Bbv12I GWGCWC 2 cut(s) 769, 798
BbvCI CCTCAGC 1 cut(s) 122
BbvI GCAGC 1 cut(s) 685
BccI CCATC 5 cut(s) 123, 235, 443, 515, 538
BciT130I CCWGG 1 cut(s) 517
BciVI GTATCC 1 cut(s) 38
BfmI CTRYAG 2 cut(s) 15, 258
BfuI GTATCC 1 cut(s) 38
BglII AGATCT 3 cut(s) 152, 214, 505
BisI GCNGC 2 cut(s) 11, 674
BlsI GCNGC 2 cut(s) 12, 675
BmcAI AGTACT 1 cut(s) 333
Bme1390I CCNGG 1 cut(s) 517
Bme18I GGWCC 2 cut(s) 252, 800
BmeT110I CYCGRG 1 cut(s) 762
BmgT120I GGNCC 2 cut(s) 252, 800
BmiI GGNNCC 3 cut(s) 390, 514, 721
BmrFI CCNGG 1 cut(s) 517
BmsI GCATC 5 cut(s) 40, 262, 306, 433, 767
BplI GAGNNNNNCTC 2 cut(s) 302, 334
BpmI CTGGAG 1 cut(s) 753
Bpu10I CCTNAGC 2 cut(s) 74, 122
BpuEI CTTGAG 1 cut(s) 336
BsaJI CCNNGG 2 cut(s) 384, 516
Bse3DI GCAATG 6 cut(s) 321, 384, 664, 706, 745, 784
BseBI CCWGG 1 cut(s) 517
BseDI CCNNGG 2 cut(s) 384, 516
BseGI GGATG 5 cut(s) 134, 297, 448, 526, 758
BseMI GCAATG 6 cut(s) 321, 384, 664, 706, 745, 784
BseMII CTCAG 3 cut(s) 113, 349, 461
BseRI GAGGAG 1 cut(s) 152
BseXI GCAGC 1 cut(s) 685
BsgI GTGCAG 1 cut(s) 773
Bsh1236I CGCG 1 cut(s) 713
BshFI GGCC 4 cut(s) 383, 453, 477, 620
BshNI GGYRCC 1 cut(s) 388
BsiHKAI GWGCWC 2 cut(s) 769, 798
BsiHKCI CYCGRG 1 cut(s) 762
BslFI GGGAC 2 cut(s) 185, 263
BsmFI GGGAC 2 cut(s) 185, 263
BsmI GAATGC 1 cut(s) 779
BsnI GGCC 4 cut(s) 383, 453, 477, 620
BsoBI CYCGRG 1 cut(s) 762
Bsp1286I GDGCHC 3 cut(s) 432, 769, 798
Bsp143I GATC 4 cut(s) 152, 214, 505, 512
Bsp19I CCATGG 1 cut(s) 384
BspACI CCGC 3 cut(s) 36, 555, 589
BspANI GGCC 4 cut(s) 383, 453, 477, 620
BspCNI CTCAG 3 cut(s) 114, 348, 462
BspFNI CGCG 1 cut(s) 713
BspLI GGNNCC 3 cut(s) 390, 514, 721
BspMAI CTGCAG 1 cut(s) 262
BspPI GGATC 2 cut(s) 507, 520
BspQI GCTCTTC 1 cut(s) 598
BspT107I GGYRCC 1 cut(s) 388
BsrBI CCGCTC 1 cut(s) 591
BsrDI GCAATG 6 cut(s) 321, 384, 664, 706, 745, 784
BssECI CCNNGG 2 cut(s) 384, 516
BssMI GATC 4 cut(s) 152, 214, 505, 512
BssT1I CCWWGG 1 cut(s) 384
Bst2UI CCWGG 1 cut(s) 517
Bst6I CTCTTC 2 cut(s) 598, 777
BstC8I GCNNGC 2 cut(s) 752, 756
BstDEI CTNAG 5 cut(s) 74, 122, 246, 335, 470
BstDSI CCRYGG 1 cut(s) 384
BstF5I GGATG 5 cut(s) 134, 297, 448, 526, 758
BstFNI CGCG 1 cut(s) 713
BstHHI GCGC 1 cut(s) 715
BstKTI GATC 4 cut(s) 155, 217, 508, 515
BstMBI GATC 4 cut(s) 152, 214, 505, 512
BstMWI GCNNNNNNNGC 1 cut(s) 764
BstNI CCWGG 1 cut(s) 517
BstSCI CCNGG 1 cut(s) 515
BstSFI CTRYAG 2 cut(s) 15, 258
BstUI CGCG 1 cut(s) 713
BstV1I GCAGC 1 cut(s) 685
BstX2I RGATCY 4 cut(s) 152, 214, 505, 512
BstYI RGATCY 4 cut(s) 152, 214, 505, 512
BsuI GTATCC 1 cut(s) 38
BsuRI GGCC 4 cut(s) 383, 453, 477, 620
BtgI CCRYGG 1 cut(s) 384
BtgZI GCGATG 1 cut(s) 285
BtsCI GGATG 5 cut(s) 134, 297, 448, 526, 758
BtsI GCAGTG 1 cut(s) 255
BtsIMutI CAGTG 1 cut(s) 255
Cac8I GCNNGC 2 cut(s) 752, 756
CfoI GCGC 1 cut(s) 715
Cfr13I GGNCC 2 cut(s) 252, 800
CseI GACGC 1 cut(s) 127
Csp6I GTAC 2 cut(s) 332, 389
CviAII CATG 4 cut(s) 361, 385, 455, 790
CviQI GTAC 2 cut(s) 332, 389
DdeI CTNAG 5 cut(s) 74, 122, 246, 335, 470
DpnI GATC 4 cut(s) 154, 216, 507, 514
DpnII GATC 4 cut(s) 152, 214, 505, 512
DrdI GACNNNNNNGTC 1 cut(s) 310
DseDI GACNNNNNNGTC 1 cut(s) 310
EaeI YGGCCR 2 cut(s) 381, 451
Eam1104I CTCTTC 2 cut(s) 598, 777
EarI CTCTTC 2 cut(s) 598, 777
Ecl136II GAGCTC 2 cut(s) 767, 796
Eco130I CCWWGG 1 cut(s) 384
Eco24I GRGCYC 3 cut(s) 432, 769, 798
Eco47I GGWCC 2 cut(s) 252, 800
Eco53kI GAGCTC 2 cut(s) 767, 796
Eco57I CTGAAG 2 cut(s) 291, 312
Eco88I CYCGRG 1 cut(s) 762
EcoICRI GAGCTC 2 cut(s) 767, 796
EcoRII CCWGG 1 cut(s) 515
EcoT14I CCWWGG 1 cut(s) 384
EcoT38I GRGCYC 3 cut(s) 432, 769, 798
ErhI CCWWGG 1 cut(s) 384
FaeI CATG 4 cut(s) 364, 388, 458, 793
FaiI YATR 7 cut(s) 165, 362, 386, 409, 456, 494, 791
FaqI GGGAC 2 cut(s) 185, 263
FatI CATG 4 cut(s) 360, 384, 454, 789
Fnu4HI GCNGC 2 cut(s) 11, 674
FokI GGATG 5 cut(s) 141, 284, 455, 533, 745
FriOI GRGCYC 3 cut(s) 432, 769, 798
Fsp4HI GCNGC 2 cut(s) 11, 674
GlaI GCGC 1 cut(s) 714
GluI GCNGC 2 cut(s) 11, 674
GsuI CTGGAG 1 cut(s) 753
HaeIII GGCC 4 cut(s) 383, 453, 477, 620
HgaI GACGC 1 cut(s) 127
HhaI GCGC 1 cut(s) 715
Hin1II CATG 4 cut(s) 364, 388, 458, 793
Hin6I GCGC 1 cut(s) 713
HinP1I GCGC 1 cut(s) 713
HindIII AAGCTT 1 cut(s) 71
HinfI GANTC 4 cut(s) 290, 311, 317, 727
HphI GGTGA 2 cut(s) 50, 548
Hpy166II GTNNAC 1 cut(s) 539
Hpy188I TCNGA 6 cut(s) 84, 289, 310, 316, 338, 694
Hpy188III TCNNGA 7 cut(s) 280, 301, 463, 503, 731, 762, 770
Hpy8I GTNNAC 1 cut(s) 539
HpyAV CCTTC 3 cut(s) 26, 469, 813
HpyCH4V TGCA 5 cut(s) 232, 260, 673, 754, 777
HpyF10VI GCNNNNNNNGC 1 cut(s) 764
HpyF3I CTNAG 5 cut(s) 74, 122, 246, 335, 470
Hsp92II CATG 4 cut(s) 364, 388, 458, 793
HspAI GCGC 1 cut(s) 713
KpnI GGTACC 1 cut(s) 392
Kzo9I GATC 4 cut(s) 152, 214, 505, 512
LguI GCTCTTC 1 cut(s) 598
LmnI GCTCC 5 cut(s) 7, 139, 628, 772, 793
Lsp1109I GCAGC 1 cut(s) 685
LweI GCATC 5 cut(s) 40, 262, 306, 433, 767
MaeIII GTNAC 1 cut(s) 638
MalI GATC 4 cut(s) 154, 216, 507, 514
MbiI CCGCTC 1 cut(s) 591
MboI GATC 4 cut(s) 152, 214, 505, 512
MboII GAAGA 6 cut(s) 500, 585, 642, 681, 716, 794
MfeI CAATTG 1 cut(s) 576
MflI RGATCY 4 cut(s) 152, 214, 505, 512
MhlI GDGCHC 3 cut(s) 432, 769, 798
MlsI TGGCCA 2 cut(s) 383, 453
MluCI AATT 4 cut(s) 576, 645, 668, 811
MluNI TGGCCA 2 cut(s) 383, 453
MlyI GAGTC 4 cut(s) 299, 311, 320, 721
MmeI TCCRAC 2 cut(s) 184, 294
Mox20I TGGCCA 2 cut(s) 383, 453
MroXI GAANNNNTTC 1 cut(s) 649
MscI TGGCCA 2 cut(s) 383, 453
MseI TTAA 3 cut(s) 56, 108, 814
MslI CAYNNNNRTG 2 cut(s) 365, 483
Msp20I TGGCCA 2 cut(s) 383, 453
MspR9I CCNGG 1 cut(s) 517
MunI CAATTG 1 cut(s) 576
Mva1269I GAATGC 1 cut(s) 779
MvaI CCWGG 1 cut(s) 517
MvnI CGCG 1 cut(s) 713
MwoI GCNNNNNNNGC 1 cut(s) 764
NcoI CCATGG 1 cut(s) 384
NdeII GATC 4 cut(s) 152, 214, 505, 512
NlaIII CATG 4 cut(s) 364, 388, 458, 793
NlaIV GGNNCC 3 cut(s) 390, 514, 721
PciSI GCTCTTC 1 cut(s) 598
PctI GAATGC 1 cut(s) 779
PdmI GAANNNNTTC 1 cut(s) 649
PkrI GCNGC 2 cut(s) 12, 675
PleI GAGTC 4 cut(s) 298, 311, 319, 721
PpsI GAGTC 4 cut(s) 298, 311, 319, 721
Psp124BI GAGCTC 2 cut(s) 769, 798
Psp6I CCWGG 1 cut(s) 515
PspGI CCWGG 1 cut(s) 515
PspN4I GGNNCC 3 cut(s) 390, 514, 721
PspPI GGNCC 2 cut(s) 252, 800
PstI CTGCAG 1 cut(s) 262
PsuI RGATCY 4 cut(s) 152, 214, 505, 512
RsaI GTAC 2 cut(s) 333, 390
RsaNI GTAC 2 cut(s) 332, 389
RseI CAYNNNNRTG 2 cut(s) 365, 483
SacI GAGCTC 2 cut(s) 769, 798
SapI GCTCTTC 1 cut(s) 598
SaqAI TTAA 3 cut(s) 56, 108, 814
SatI GCNGC 2 cut(s) 11, 674
Sau3AI GATC 4 cut(s) 152, 214, 505, 512
Sau96I GGNCC 2 cut(s) 252, 800
ScaI AGTACT 1 cut(s) 333
SchI GAGTC 4 cut(s) 299, 311, 320, 721
ScrFI CCNGG 1 cut(s) 517
SduI GDGCHC 3 cut(s) 432, 769, 798
SetI ASST 8 cut(s) 12, 75, 352, 358, 369, 678, 769, 798
SfaNI GCATC 5 cut(s) 40, 262, 306, 433, 767
SfcI CTRYAG 2 cut(s) 15, 258
SinI GGWCC 2 cut(s) 252, 800
SmiMI CAYNNNNRTG 2 cut(s) 365, 483
SmlI CTYRAG 1 cut(s) 351
SmoI CTYRAG 1 cut(s) 351
Sse9I AATT 4 cut(s) 576, 645, 668, 811
SsiI CCGC 3 cut(s) 36, 555, 589
SstI GAGCTC 2 cut(s) 769, 798
StyD4I CCNGG 1 cut(s) 515
StyI CCWWGG 1 cut(s) 384
TaqI TCGA 2 cut(s) 293, 372
TaqII GACCGA 1 cut(s) 788
TasI AATT 4 cut(s) 576, 645, 668, 811
TatI WGTACW 1 cut(s) 331
Tru1I TTAA 3 cut(s) 56, 108, 814
Tru9I TTAA 3 cut(s) 56, 108, 814
TscAI CASTG 1 cut(s) 262
TseI GCWGC 2 cut(s) 10, 673
TspDTI ATGAA 7 cut(s) 17, 162, 501, 581, 678, 717, 795
TspRI CASTG 1 cut(s) 262
VpaK11BI GGWCC 2 cut(s) 252, 800
XapI RAATTY 1 cut(s) 645
XmnI GAANNNNTTC 1 cut(s) 649
ZrmI AGTACT 1 cut(s) 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.