RchiOBHm_Chr2g0158861

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001118
Physical Location & Seq
Reverse (-)
75016185 .. 75017004
820 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 618 bp
ATGACCCTAACCAGATTACACAAAGCAATTTCAAAAGCTTCTTGTATTCCTAAGCTATACTTCTTTGGGTTCCCAATTAGCCACATTGATCTTTCAATGGCATCCCCCCTACTACTACTGTTGGTTTTTTCCATGAGTGCTAGTGCACTTTTAGTCCAAGCCAGTTATGTCAAAGAAACCCGAATGGTCTTGTACTTCCAAGACATCTCGACAGGTCCAAATGCCACAAGCATACCAATTGCAGGCATTGCCGGAAAGCTTTGGACCTTCACTCAGTTTGGAACAGTTTATGTCACTGACGACACCATGACCGAAGGTCCAAGCCCAAAGTCAGCCGTAGTTGGCCGGGCACAAGGCGTGTCAGTGGCAGCAGCGCTAGACGGGAGTAATGCCCTTGTCTTGGTATCGCTTGTGTTCACTAATAAGGAGTACAATGGCAGCACTATAGAGATACAAGGAAACAGTAAGCAATTCGAGTCTGTCAGAGAGGTTGCAGTGGTATCAGGTACCGGGAAATTCAGGTTTGCTAGAGGATATGCTACCTTTGAATCATACTTCGTGGATCTTCCGAGTGCCTACTCAATATTACGGTGCAATGTTACAGTGCAACATTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

205

Amino Acids

22.21

Weight (kDa)

8.85

Isoelectric Point (pI)

30.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 60 - 202 1.9e-47 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 506
AccB1I GGYRCC 1 cut(s) 506
AclWI GGATC 1 cut(s) 570
AcoI YGGCCR 1 cut(s) 343
AcsI RAATTY 1 cut(s) 515
AfaI GTAC 3 cut(s) 194, 431, 508
AfeI AGCGCT 1 cut(s) 375
AfiI CCNNNNNNNGG 2 cut(s) 242, 400
AgsI TTSAA 3 cut(s) 33, 96, 548
AhdI GACNNNNNGTC 1 cut(s) 315
AluBI AGCT 3 cut(s) 38, 55, 259
AluI AGCT 3 cut(s) 38, 55, 259
Alw21I GWGCWC 1 cut(s) 148
Alw44I GTGCAC 1 cut(s) 144
AlwI GGATC 1 cut(s) 570
Aor51HI AGCGCT 1 cut(s) 375
AoxI GGCC 1 cut(s) 343
ApaLI GTGCAC 1 cut(s) 144
ApeKI GCWGC 3 cut(s) 368, 371, 438
ApoI RAATTY 1 cut(s) 515
Asp718I GGTACC 1 cut(s) 506
AspLEI GCGC 1 cut(s) 376
AspS9I GGNCC 3 cut(s) 215, 264, 317
AsuC2I CCSGG 2 cut(s) 347, 511
AvaII GGWCC 3 cut(s) 215, 264, 317
BaeGI GKGCMC 2 cut(s) 148, 352
BanI GGYRCC 1 cut(s) 506
Bbv12I GWGCWC 1 cut(s) 148
BbvI GCAGC 3 cut(s) 380, 383, 450
BceAI ACGGC 1 cut(s) 320
BcnI CCSGG 2 cut(s) 347, 511
BfaI CTAG 4 cut(s) 141, 377, 528, 616
BfmI CTRYAG 1 cut(s) 444
BfoI RGCGCY 1 cut(s) 377
BisI GCNGC 3 cut(s) 369, 372, 439
BlsI GCNGC 3 cut(s) 370, 373, 440
Bme1390I CCNGG 2 cut(s) 347, 511
Bme18I GGWCC 3 cut(s) 215, 264, 317
BmeRI GACNNNNNGTC 1 cut(s) 315
BmgT120I GGNCC 3 cut(s) 215, 264, 317
BmiI GGNNCC 2 cut(s) 71, 508
BmrFI CCNGG 2 cut(s) 347, 511
BmsI GCATC 1 cut(s) 110
Bpu10I CCTNAGC 1 cut(s) 51
BpuMI CCSGG 2 cut(s) 347, 511
Bsc4I CCNNNNNNNGG 2 cut(s) 242, 400
Bse1I ACTGG 1 cut(s) 162
Bse3DI GCAATG 2 cut(s) 246, 601
BseGI GGATG 1 cut(s) 101
BseLI CCNNNNNNNGG 2 cut(s) 242, 400
BseMI GCAATG 2 cut(s) 246, 601
BseMII CTCAG 1 cut(s) 287
BseNI ACTGG 1 cut(s) 162
BseSI GKGCMC 2 cut(s) 148, 352
BseXI GCAGC 3 cut(s) 380, 383, 450
BshFI GGCC 1 cut(s) 345
BshNI GGYRCC 1 cut(s) 506
BsiHKAI GWGCWC 1 cut(s) 148
BsiSI CCGG 3 cut(s) 252, 346, 510
BslI CCNNNNNNNGG 2 cut(s) 242, 400
BsnI GGCC 1 cut(s) 345
Bsp1286I GDGCHC 2 cut(s) 148, 352
Bsp143I GATC 2 cut(s) 88, 562
BspANI GGCC 1 cut(s) 345
BspCNI CTCAG 1 cut(s) 286
BspLI GGNNCC 2 cut(s) 71, 508
BspPI GGATC 1 cut(s) 570
BspT107I GGYRCC 1 cut(s) 506
BsrDI GCAATG 2 cut(s) 246, 601
BsrI ACTGG 1 cut(s) 162
BssMI GATC 2 cut(s) 88, 562
Bst4CI ACNGT 5 cut(s) 120, 286, 464, 591, 604
BstAPI GCANNNNNTGC 1 cut(s) 248
BstC8I GCNNGC 1 cut(s) 244
BstDEI CTNAG 2 cut(s) 51, 273
BstF5I GGATG 1 cut(s) 101
BstH2I RGCGCY 1 cut(s) 377
BstHHI GCGC 1 cut(s) 376
BstKTI GATC 2 cut(s) 91, 565
BstMBI GATC 2 cut(s) 88, 562
BstMWI GCNNNNNNNGC 1 cut(s) 248
BstSCI CCNGG 2 cut(s) 345, 509
BstSFI CTRYAG 1 cut(s) 444
BstSLI GKGCMC 2 cut(s) 148, 352
BstV1I GCAGC 3 cut(s) 380, 383, 450
BstX2I RGATCY 1 cut(s) 562
BstYI RGATCY 1 cut(s) 562
BsuRI GGCC 1 cut(s) 345
BtsCI GGATG 1 cut(s) 101
BtsI GCAGTG 1 cut(s) 501
BtsIMutI CAGTG 4 cut(s) 294, 369, 501, 609
Cac8I GCNNGC 1 cut(s) 244
CfoI GCGC 1 cut(s) 376
Cfr13I GGNCC 3 cut(s) 215, 264, 317
Csp6I GTAC 3 cut(s) 193, 430, 507
CviAII CATG 2 cut(s) 133, 307
CviJI RGCY 8 cut(s) 38, 55, 81, 161, 259, 324, 335, 345
CviKI_1 RGCY 8 cut(s) 38, 55, 81, 161, 259, 324, 335, 345
CviQI GTAC 3 cut(s) 193, 430, 507
DdeI CTNAG 2 cut(s) 51, 273
DpnI GATC 2 cut(s) 90, 564
DpnII GATC 2 cut(s) 88, 562
DriI GACNNNNNGTC 1 cut(s) 315
EaeI YGGCCR 1 cut(s) 343
Eam1105I GACNNNNNGTC 1 cut(s) 315
Eco47I GGWCC 3 cut(s) 215, 264, 317
Eco47III AGCGCT 1 cut(s) 375
FaeI CATG 2 cut(s) 136, 310
FaiI YATR 9 cut(s) 58, 134, 168, 233, 291, 308, 446, 537, 553
FalI AAGNNNNNCTT 2 cut(s) 44, 76
FatI CATG 2 cut(s) 132, 306
Fnu4HI GCNGC 3 cut(s) 369, 372, 439
FokI GGATG 1 cut(s) 88
Fsp4HI GCNGC 3 cut(s) 369, 372, 439
FspBI CTAG 4 cut(s) 141, 377, 528, 616
GlaI GCGC 1 cut(s) 375
GluI GCNGC 3 cut(s) 369, 372, 439
HaeII RGCGCY 1 cut(s) 377
HaeIII GGCC 1 cut(s) 345
HapII CCGG 3 cut(s) 252, 346, 510
HhaI GCGC 1 cut(s) 376
Hin1II CATG 2 cut(s) 136, 310
Hin6I GCGC 1 cut(s) 374
HinP1I GCGC 1 cut(s) 374
HindIII AAGCTT 2 cut(s) 36, 257
HinfI GANTC 2 cut(s) 476, 548
HpaII CCGG 3 cut(s) 252, 346, 510
Hpy166II GTNNAC 2 cut(s) 146, 417
Hpy188I TCNGA 2 cut(s) 485, 570
Hpy188III TCNNGA 1 cut(s) 208
Hpy8I GTNNAC 2 cut(s) 146, 417
HpyAV CCTTC 2 cut(s) 277, 308
HpyCH4III ACNGT 5 cut(s) 120, 286, 464, 591, 604
HpyCH4V TGCA 5 cut(s) 146, 242, 494, 594, 607
HpyF10VI GCNNNNNNNGC 1 cut(s) 248
HpyF3I CTNAG 2 cut(s) 51, 273
Hsp92II CATG 2 cut(s) 136, 310
HspAI GCGC 1 cut(s) 374
KpnI GGTACC 1 cut(s) 510
Kzo9I GATC 2 cut(s) 88, 562
LpnPI CCDG 9 cut(s) 25, 175, 198, 228, 265, 359, 489, 505, 523
Lsp1109I GCAGC 3 cut(s) 380, 383, 450
LweI GCATC 1 cut(s) 110
MaeI CTAG 4 cut(s) 141, 377, 528, 616
MaeIII GTNAC 2 cut(s) 292, 598
MalI GATC 2 cut(s) 90, 564
MboI GATC 2 cut(s) 88, 562
MboII GAAGA 1 cut(s) 557
MfeI CAATTG 1 cut(s) 237
MflI RGATCY 1 cut(s) 562
MhlI GDGCHC 2 cut(s) 148, 352
MluCI AATT 5 cut(s) 27, 75, 237, 470, 515
MlyI GAGTC 1 cut(s) 485
MnlI CCTC 2 cut(s) 481, 524
MspI CCGG 3 cut(s) 252, 346, 510
MspR9I CCNGG 2 cut(s) 347, 511
MunI CAATTG 1 cut(s) 237
MwoI GCNNNNNNNGC 1 cut(s) 248
NciI CCSGG 2 cut(s) 347, 511
NdeII GATC 2 cut(s) 88, 562
NlaIII CATG 2 cut(s) 136, 310
NlaIV GGNNCC 2 cut(s) 71, 508
NmuCI GTSAC 1 cut(s) 292
PfeI GAWTC 1 cut(s) 548
PkrI GCNGC 3 cut(s) 370, 373, 440
PleI GAGTC 1 cut(s) 484
PpsI GAGTC 1 cut(s) 484
PspN4I GGNNCC 2 cut(s) 71, 508
PspPI GGNCC 3 cut(s) 215, 264, 317
PsuI RGATCY 1 cut(s) 562
RsaI GTAC 3 cut(s) 194, 431, 508
RsaNI GTAC 3 cut(s) 193, 430, 507
SatI GCNGC 3 cut(s) 369, 372, 439
Sau3AI GATC 2 cut(s) 88, 562
Sau96I GGNCC 3 cut(s) 215, 264, 317
SchI GAGTC 1 cut(s) 485
ScrFI CCNGG 2 cut(s) 347, 511
SduI GDGCHC 2 cut(s) 148, 352
SfaNI GCATC 1 cut(s) 110
SfcI CTRYAG 1 cut(s) 444
SinI GGWCC 3 cut(s) 215, 264, 317
Sse9I AATT 5 cut(s) 27, 75, 237, 470, 515
SspI AATATT 1 cut(s) 585
SspMI CTAG 4 cut(s) 141, 377, 528, 616
StyD4I CCNGG 2 cut(s) 345, 509
TaaI ACNGT 5 cut(s) 120, 286, 464, 591, 604
TaqI TCGA 2 cut(s) 209, 474
TaqII GACCGA 1 cut(s) 326
TasI AATT 5 cut(s) 27, 75, 237, 470, 515
TatI WGTACW 2 cut(s) 192, 429
TfiI GAWTC 1 cut(s) 548
TscAI CASTG 4 cut(s) 301, 369, 501, 609
TseFI GTSAC 1 cut(s) 292
TseI GCWGC 3 cut(s) 368, 371, 438
Tsp45I GTSAC 1 cut(s) 292
TspRI CASTG 4 cut(s) 301, 369, 501, 609
VneI GTGCAC 1 cut(s) 144
VpaK11BI GGWCC 3 cut(s) 215, 264, 317
XapI RAATTY 1 cut(s) 515
XspI CTAG 4 cut(s) 141, 377, 528, 616
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.