RchiOBHm_Chr1g0324041

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000830
Physical Location & Seq
Reverse (-)
11533114 .. 11535470
2357 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1551 bp
ATGAACAGCTCTGGCTCTCCACACGGGCATCATCATGACGAAGATATTGAGGAAAACATCATCAGCCAACAAAATCCTTCGAAAGGAGGCTGGACTGCTGCCATCTTCATCATCTTTGTCGAAATGGCAGAGAGATTTGCTTACTATGGTATGTCTGGTAACCTCATCACCTACCTCACAAACGAACTCGAGGAACCAATTCCTATGGCTGCCAAAAACGTTAACACTTGGACTGGTGTCTCGGCTCTCTTACCTCTTCTCGGAGCCTTCATAGCCGATTCCTTTCTCGGTCGATTCACCACCATCCTTGTCTCCTCCATTATTTACTGCATGGGAATGAGTTTGTTGACCGTAACAGTTTCGGTTGTTCCACTGCATTTGCGCAAAGCAATGTTCTTTGTGGCACTTTACATATTGTCAGTTGGGCTAGCCGGGCACAAACCATGTGTGCAGACGTTTGCGGCGGACCAGTTTTCTGAGGACTCGCCAGAGGAAAGAAAGGCAAAAAGCTCCTTCTTCAACTGGTGGTACCTGGGGATTATTGTTGCTGGCTCTGCTGCCATATTGGTAGTCATATATATCCAGGACAATGTAAGCTGGGCGGCAGGATTTGGAATAATGACAGGTTCCATGGTGGTGGCACTAATTTTATTTCTATTGGGAATCAAGAAATACCGAAAGCAAGGACCCCTAGGCAGTCGTCAGGAAGCGGCACACCGAGTTGAAAACTATAGATTTTTGGACAAGGCAATGGTCATTGACCACTTGGATGCTTCAAGCGAGGCCAGAAATCCTTGGAGACTATGTTCACTAAATCAAGTGGAACAAGTAAAGCTCCTCCTACGCCTCATTCCCATATGGATGTGCTGCTTAATGTTTGCTGTAGTCCAGTCATTTGTGCAGACCTTCTTCACCAAACAAGGAAGCACAATGATCCGGTCAATAGGTTCATTTCAGATTCCCCAAGCTTCCCTCCTAATCTTCGTTGGTCTCACCATCATAGTTGTCATTCCAATCTACGACCGTGTTTTTGTCCCAACTGCCCGAAAATTCACCGGACATCCCTCAGGTATTACCATGCTACAAAGAATTGGCACTGGCCTATTAATATCAATAATCACTATGGTTGTTGCAGCTGTGGTAGAAGCCAAAAGGGTAAAGATTTTAGGAGATCACAACCTCCTGGACAATCCGAAAGCAATTGTTCCAATGAGAGTGTGGTGGTTAGTTCCTCAGTATGTGATATCTGGTTTGTCTGATGTATTTGCGTTTGTTGGAATCCAAGAGTTGTTCTATGATCAAATGCCGGAGGCAATGAGAAGCTTGGGAGCAGCATTTTACCTCAGCGTCACCGGAATTGGAAACTTCATAAGCAGTGGGATAATAACGTTGGTTCAAGTAATTAGCTCTAAGTATGGTGAAAAATGGATTAGTGACAATATCAATCACGCACACCTCGATTACTTTTACTGGGTAATTGCAGCTCTGAGCACATTGAATTTGTGCATTTACGTGTGGATTGCTAAAGGATTTCTATACAAAAAAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

516

Amino Acids

57.43

Weight (kDa)

8.57

Isoelectric Point (pI)

32.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 58 - 458 8.4e-13 Major Facilitator Superfamily
PTR2 PF00854 99 - 472 1.1e-79 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 383
Acc65I GGTACC 1 cut(s) 528
AccB1I GGYRCC 1 cut(s) 528
AciI CCGC 4 cut(s) 461, 464, 602, 710
AclI AACGTT 2 cut(s) 219, 1388
AclWI GGATC 1 cut(s) 928
AcsI RAATTY 2 cut(s) 1049, 1498
AfaI GTAC 1 cut(s) 530
AfiI CCNNNNNNNGG 2 cut(s) 83, 260
AflIII ACRYGT 1 cut(s) 1512
AgsI TTSAA 5 cut(s) 520, 725, 777, 1397, 1498
AjnI CCWGG 3 cut(s) 531, 582, 1182
AloI GAACNNNNNNTCC 2 cut(s) 790, 822
AluBI AGCT 9 cut(s) 9, 510, 597, 835, 968, 1136, 1323, 1407, 1484
AluI AGCT 9 cut(s) 9, 510, 597, 835, 968, 1136, 1323, 1407, 1484
Alw21I GWGCWC 1 cut(s) 1493
Alw26I GTCTC 4 cut(s) 244, 316, 793, 995
AlwI GGATC 1 cut(s) 928
Ama87I CYCGRG 1 cut(s) 188
AoxI GGCC 2 cut(s) 783, 1099
ApeKI GCWGC 7 cut(s) 98, 209, 557, 867, 1133, 1331, 1481
ApoI RAATTY 2 cut(s) 1049, 1498
ArsI GACNNNNNNTTYG 2 cut(s) 1013, 1045
AseI ATTAAT 1 cut(s) 1106
Asp700I GAANNNNTTC 1 cut(s) 198
Asp718I GGTACC 1 cut(s) 528
AspA2I CCTAGG 1 cut(s) 691
AspLEI GCGC 1 cut(s) 384
AspS9I GGNCC 2 cut(s) 466, 686
AsuC2I CCSGG 1 cut(s) 433
AsuHPI GGTGA 7 cut(s) 160, 289, 904, 985, 1045, 1342, 1430
AsuII TTCGAA 1 cut(s) 80
AsuNHI GCTAGC 1 cut(s) 427
AvaI CYCGRG 1 cut(s) 188
AvaII GGWCC 2 cut(s) 466, 686
AvrII CCTAGG 1 cut(s) 691
AxyI CCTNAGG 1 cut(s) 1066
BaeGI GKGCMC 1 cut(s) 438
BanI GGYRCC 1 cut(s) 528
Bbv12I GWGCWC 1 cut(s) 1493
BbvCI CCTCAGC 1 cut(s) 1343
BbvI GCAGC 7 cut(s) 85, 196, 544, 854, 1145, 1343, 1493
BccI CCATC 3 cut(s) 110, 311, 1004
BciT130I CCWGG 3 cut(s) 533, 584, 1184
BclI TGATCA 1 cut(s) 1297
BcnI CCSGG 1 cut(s) 433
BcoDI GTCTC 4 cut(s) 244, 316, 793, 995
BfaI CTAG 2 cut(s) 428, 692
BfmI CTRYAG 2 cut(s) 730, 882
BlnI CCTAGG 1 cut(s) 691
Bme1390I CCNGG 4 cut(s) 433, 533, 584, 1184
Bme18I GGWCC 2 cut(s) 466, 686
BmeT110I CYCGRG 1 cut(s) 188
BmgT120I GGNCC 2 cut(s) 466, 686
BmiI GGNNCC 5 cut(s) 195, 265, 530, 628, 688
BmrFI CCNGG 4 cut(s) 433, 533, 584, 1184
BmrI ACTGGG 1 cut(s) 1480
BmsI GCATC 2 cut(s) 37, 760
BmtI GCTAGC 1 cut(s) 431
BmuI ACTGGG 1 cut(s) 1480
BoxI GACNNNNGTC 1 cut(s) 236
Bpu10I CCTNAGC 1 cut(s) 1343
Bpu14I TTCGAA 1 cut(s) 80
BpuMI CCSGG 1 cut(s) 433
BsaAI YACGTR 1 cut(s) 1513
BsaI GGTCTC 1 cut(s) 995
BsaJI CCNNGG 4 cut(s) 532, 630, 691, 794
BsaWI WCCGGW 3 cut(s) 936, 1055, 1352
BsaXI ACNNNNNCTCC 2 cut(s) 790, 820
Bsc4I CCNNNNNNNGG 2 cut(s) 83, 260
Bse1I ACTGG 6 cut(s) 238, 469, 527, 889, 1102, 1475
Bse21I CCTNAGG 1 cut(s) 1066
Bse3DI GCAATG 3 cut(s) 396, 756, 1320
BseBI CCWGG 3 cut(s) 533, 584, 1184
BseDI CCNNGG 4 cut(s) 532, 630, 691, 794
BseGI GGATG 4 cut(s) 303, 775, 867, 1060
BseLI CCNNNNNNNGG 2 cut(s) 83, 260
BseMI GCAATG 3 cut(s) 396, 756, 1320
BseMII CTCAG 5 cut(s) 468, 1080, 1247, 1357, 1478
BseNI ACTGG 6 cut(s) 238, 469, 527, 889, 1102, 1475
BseRI GAGGAG 2 cut(s) 304, 827
BseSI GKGCMC 1 cut(s) 438
BseXI GCAGC 7 cut(s) 85, 196, 544, 854, 1145, 1343, 1493
BseYI CCCAGC 1 cut(s) 597
BsgI GTGCAG 2 cut(s) 470, 920
Bsh1285I CGRYCG 2 cut(s) 292, 1024
BshFI GGCC 2 cut(s) 785, 1101
BshNI GGYRCC 1 cut(s) 528
BsiEI CGRYCG 2 cut(s) 292, 1024
BsiHKAI GWGCWC 1 cut(s) 1493
BsiHKCI CYCGRG 1 cut(s) 188
BsiSI CCGG 5 cut(s) 432, 937, 1056, 1307, 1353
BslFI GGGAC 1 cut(s) 1019
BslI CCNNNNNNNGG 2 cut(s) 83, 260
BsmAI GTCTC 4 cut(s) 244, 316, 793, 995
BsmFI GGGAC 1 cut(s) 1019
BsnI GGCC 2 cut(s) 785, 1101
Bso31I GGTCTC 1 cut(s) 995
BsoBI CYCGRG 1 cut(s) 188
Bsp119I TTCGAA 1 cut(s) 80
Bsp1286I GDGCHC 2 cut(s) 438, 1493
Bsp143I GATC 3 cut(s) 933, 1171, 1297
Bsp19I CCATGG 1 cut(s) 630
BspACI CCGC 4 cut(s) 461, 464, 602, 710
BspANI GGCC 2 cut(s) 785, 1101
BspCNI CTCAG 5 cut(s) 469, 1079, 1246, 1356, 1479
BspHI TCATGA 1 cut(s) 34
BspLI GGNNCC 5 cut(s) 195, 265, 530, 628, 688
BspOI GCTAGC 1 cut(s) 431
BspPI GGATC 1 cut(s) 928
BspT104I TTCGAA 1 cut(s) 80
BspT107I GGYRCC 1 cut(s) 528
BspTNI GGTCTC 1 cut(s) 995
BsrDI GCAATG 3 cut(s) 396, 756, 1320
BsrI ACTGG 6 cut(s) 238, 469, 527, 889, 1102, 1475
BssECI CCNNGG 4 cut(s) 532, 630, 691, 794
BssMI GATC 3 cut(s) 933, 1171, 1297
BssT1I CCWWGG 3 cut(s) 630, 691, 794
Bst2UI CCWGG 3 cut(s) 533, 584, 1184
Bst4CI ACNGT 3 cut(s) 352, 358, 1025
Bst6I CTCTTC 1 cut(s) 261
BstBAI YACGTR 1 cut(s) 1513
BstBI TTCGAA 1 cut(s) 80
BstC8I GCNNGC 2 cut(s) 429, 550
BstDEI CTNAG 6 cut(s) 477, 1066, 1233, 1343, 1410, 1487
BstDSI CCRYGG 1 cut(s) 630
BstEII GGTNACC 1 cut(s) 158
BstENI CCTNNNNNAGG 1 cut(s) 81
BstF5I GGATG 4 cut(s) 303, 775, 867, 1060
BstHHI GCGC 1 cut(s) 384
BstKTI GATC 3 cut(s) 936, 1174, 1300
BstMAI GTCTC 4 cut(s) 244, 316, 793, 995
BstMBI GATC 3 cut(s) 933, 1171, 1297
BstMCI CGRYCG 2 cut(s) 292, 1024
BstMWI GCNNNNNNNGC 3 cut(s) 272, 433, 554
BstNI CCWGG 3 cut(s) 533, 584, 1184
BstPAI GACNNNNGTC 1 cut(s) 236
BstPI GGTNACC 1 cut(s) 158
BstSCI CCNGG 4 cut(s) 431, 531, 582, 1182
BstSFI CTRYAG 2 cut(s) 730, 882
BstSLI GKGCMC 1 cut(s) 438
BstV1I GCAGC 7 cut(s) 85, 196, 544, 854, 1145, 1343, 1493
BstXI CCANNNNNNTGG 1 cut(s) 637
Bsu36I CCTNAGG 1 cut(s) 1066
BsuRI GGCC 2 cut(s) 785, 1101
BtgI CCRYGG 1 cut(s) 630
BtsCI GGATG 4 cut(s) 303, 775, 867, 1060
BtsI GCAGTG 2 cut(s) 371, 1381
BtsIMutI CAGTG 3 cut(s) 371, 1095, 1381
Cac8I GCNNGC 2 cut(s) 429, 550
CciI TCATGA 1 cut(s) 34
CfoI GCGC 1 cut(s) 384
Cfr13I GGNCC 2 cut(s) 466, 686
CseI GACGC 1 cut(s) 1336
Csp6I GTAC 1 cut(s) 529
CspCI CAANNNNNGTGG 4 cut(s) 289, 324, 360, 395
CviAII CATG 5 cut(s) 35, 331, 444, 631, 1078
CviQI GTAC 1 cut(s) 529
DdeI CTNAG 6 cut(s) 477, 1066, 1233, 1343, 1410, 1487
DpnI GATC 3 cut(s) 935, 1173, 1299
DpnII GATC 3 cut(s) 933, 1171, 1297
Eam1104I CTCTTC 1 cut(s) 261
EarI CTCTTC 1 cut(s) 261
EciI GGCGGA 1 cut(s) 479
Eco130I CCWWGG 3 cut(s) 630, 691, 794
Eco31I GGTCTC 1 cut(s) 995
Eco32I GATATC 1 cut(s) 1245
Eco47I GGWCC 2 cut(s) 466, 686
Eco81I CCTNAGG 1 cut(s) 1066
Eco88I CYCGRG 1 cut(s) 188
Eco91I GGTNACC 1 cut(s) 158
EcoNI CCTNNNNNAGG 1 cut(s) 81
EcoO109I RGGNCCY 1 cut(s) 686
EcoO65I GGTNACC 1 cut(s) 158
EcoRII CCWGG 3 cut(s) 531, 582, 1182
EcoRV GATATC 1 cut(s) 1245
EcoT14I CCWWGG 3 cut(s) 630, 691, 794
ErhI CCWWGG 3 cut(s) 630, 691, 794
FaeI CATG 5 cut(s) 38, 334, 447, 634, 1081
FaqI GGGAC 1 cut(s) 1019
FatI CATG 5 cut(s) 34, 330, 443, 630, 1077
FauNDI CATATG 1 cut(s) 857
FbaI TGATCA 1 cut(s) 1297
FokI GGATG 4 cut(s) 290, 782, 874, 1047
FspBI CTAG 2 cut(s) 428, 692
FspI TGCGCA 1 cut(s) 383
GlaI GCGC 1 cut(s) 383
GsaI CCCAGC 1 cut(s) 601
HaeIII GGCC 2 cut(s) 785, 1101
HapII CCGG 5 cut(s) 432, 937, 1056, 1307, 1353
HgaI GACGC 1 cut(s) 1336
HhaI GCGC 1 cut(s) 384
Hin1II CATG 5 cut(s) 38, 334, 447, 634, 1081
Hin6I GCGC 1 cut(s) 382
HinP1I GCGC 1 cut(s) 382
HincII GTYRAC 2 cut(s) 223, 348
HindII GTYRAC 2 cut(s) 223, 348
HindIII AAGCTT 2 cut(s) 966, 1321
HinfI GANTC 6 cut(s) 278, 294, 482, 663, 958, 1278
HpaI GTTAAC 1 cut(s) 223
HpaII CCGG 5 cut(s) 432, 937, 1056, 1307, 1353
HphI GGTGA 7 cut(s) 160, 289, 904, 985, 1045, 1342, 1430
Hpy166II GTNNAC 3 cut(s) 223, 348, 809
Hpy188I TCNGA 6 cut(s) 263, 478, 957, 1194, 1258, 1488
Hpy188III TCNNGA 3 cut(s) 35, 667, 704
Hpy8I GTNNAC 3 cut(s) 223, 348, 809
HpyAV CCTTC 4 cut(s) 87, 277, 523, 916
HpyCH4III ACNGT 3 cut(s) 352, 358, 1025
HpyCH4IV ACGT 4 cut(s) 219, 455, 1388, 1512
HpyCH4V TGCA 7 cut(s) 330, 376, 451, 901, 1133, 1481, 1506
HpyF10VI GCNNNNNNNGC 3 cut(s) 272, 433, 554
HpyF3I CTNAG 6 cut(s) 477, 1066, 1233, 1343, 1410, 1487
HpySE526I ACGT 4 cut(s) 219, 455, 1388, 1512
Hsp92II CATG 5 cut(s) 38, 334, 447, 634, 1081
HspAI GCGC 1 cut(s) 382
KpnI GGTACC 1 cut(s) 532
Ksp22I TGATCA 1 cut(s) 1297
KspAI GTTAAC 1 cut(s) 223
Kzo9I GATC 3 cut(s) 933, 1171, 1297
LmnI GCTCC 4 cut(s) 263, 515, 840, 1328
Lsp1109I GCAGC 7 cut(s) 85, 196, 544, 854, 1145, 1343, 1493
LweI GCATC 2 cut(s) 37, 760
MaeI CTAG 2 cut(s) 428, 692
MaeII ACGT 4 cut(s) 219, 455, 1388, 1512
MaeIII GTNAC 4 cut(s) 158, 352, 1348, 1433
MalI GATC 3 cut(s) 935, 1173, 1299
MboI GATC 3 cut(s) 933, 1171, 1297
MboII GAAGA 6 cut(s) 53, 97, 248, 508, 901, 973
MfeI CAATTG 1 cut(s) 1200
MhlI GDGCHC 2 cut(s) 438, 1493
MluCI AATT 9 cut(s) 198, 645, 1049, 1089, 1200, 1356, 1401, 1476, 1498
MlyI GAGTC 1 cut(s) 476
MmeI TCCRAC 1 cut(s) 1255
MroXI GAANNNNTTC 1 cut(s) 198
MseI TTAA 3 cut(s) 222, 872, 1106
MslI CAYNNNNRTG 6 cut(s) 33, 335, 635, 768, 860, 1511
MspA1I CMGCKG 1 cut(s) 1136
MspI CCGG 5 cut(s) 432, 937, 1056, 1307, 1353
MspR9I CCNGG 4 cut(s) 433, 533, 584, 1184
MunI CAATTG 1 cut(s) 1200
MvaI CCWGG 3 cut(s) 533, 584, 1184
MwoI GCNNNNNNNGC 3 cut(s) 272, 433, 554
NciI CCSGG 1 cut(s) 433
NcoI CCATGG 1 cut(s) 630
NdeI CATATG 1 cut(s) 857
NdeII GATC 3 cut(s) 933, 1171, 1297
NheI GCTAGC 1 cut(s) 427
NlaIII CATG 5 cut(s) 38, 334, 447, 634, 1081
NlaIV GGNNCC 5 cut(s) 195, 265, 530, 628, 688
NmeAIII GCCGAG 1 cut(s) 221
NmuCI GTSAC 2 cut(s) 1348, 1433
NsbI TGCGCA 1 cut(s) 383
NspV TTCGAA 1 cut(s) 80
PaeR7I CTCGAG 1 cut(s) 188
PagI TCATGA 1 cut(s) 34
PcsI WCGNNNNNNNCGW 1 cut(s) 1455
PdmI GAANNNNTTC 1 cut(s) 198
PfeI GAWTC 5 cut(s) 278, 294, 663, 958, 1278
PfoI TCCNGGA 2 cut(s) 582, 1182
PleI GAGTC 1 cut(s) 476
PpsI GAGTC 1 cut(s) 476
Ppu21I YACGTR 1 cut(s) 1513
PpuMI RGGWCCY 1 cut(s) 686
PshAI GACNNNNGTC 1 cut(s) 236
PshBI ATTAAT 1 cut(s) 1106
Psp1406I AACGTT 2 cut(s) 219, 1388
Psp5II RGGWCCY 1 cut(s) 686
Psp6I CCWGG 3 cut(s) 531, 582, 1182
PspEI GGTNACC 1 cut(s) 158
PspFI CCCAGC 1 cut(s) 597
PspGI CCWGG 3 cut(s) 531, 582, 1182
PspN4I GGNNCC 5 cut(s) 195, 265, 530, 628, 688
PspPI GGNCC 2 cut(s) 466, 686
PspPPI RGGWCCY 1 cut(s) 686
PspXI VCTCGAGB 1 cut(s) 188
PvuII CAGCTG 1 cut(s) 1136
RsaI GTAC 1 cut(s) 530
RsaNI GTAC 1 cut(s) 529
RseI CAYNNNNRTG 6 cut(s) 33, 335, 635, 768, 860, 1511
SaqAI TTAA 3 cut(s) 222, 872, 1106
Sau3AI GATC 3 cut(s) 933, 1171, 1297
Sau96I GGNCC 2 cut(s) 466, 686
SchI GAGTC 1 cut(s) 476
ScrFI CCNGG 4 cut(s) 433, 533, 584, 1184
SduI GDGCHC 2 cut(s) 438, 1493
SfaNI GCATC 2 cut(s) 37, 760
SfcI CTRYAG 2 cut(s) 730, 882
Sfr274I CTCGAG 1 cut(s) 188
SfuI TTCGAA 1 cut(s) 80
SinI GGWCC 2 cut(s) 466, 686
SlaI CTCGAG 1 cut(s) 188
SmiMI CAYNNNNRTG 6 cut(s) 33, 335, 635, 768, 860, 1511
SmlI CTYRAG 1 cut(s) 188
SmoI CTYRAG 1 cut(s) 188
Sse9I AATT 9 cut(s) 198, 645, 1049, 1089, 1200, 1356, 1401, 1476, 1498
SsiI CCGC 4 cut(s) 461, 464, 602, 710
SspMI CTAG 2 cut(s) 428, 692
StyD4I CCNGG 4 cut(s) 431, 531, 582, 1182
StyI CCWWGG 3 cut(s) 630, 691, 794
TaaI ACNGT 3 cut(s) 352, 358, 1025
TaiI ACGT 4 cut(s) 222, 458, 1391, 1515
TaqI TCGA 5 cut(s) 80, 120, 189, 292, 1458
TaqII GACCGA 1 cut(s) 278
TasI AATT 9 cut(s) 198, 645, 1049, 1089, 1200, 1356, 1401, 1476, 1498
TauI GCSGC 3 cut(s) 464, 605, 713
TfiI GAWTC 5 cut(s) 278, 294, 663, 958, 1278
Tru1I TTAA 3 cut(s) 222, 872, 1106
Tru9I TTAA 3 cut(s) 222, 872, 1106
TscAI CASTG 3 cut(s) 378, 1102, 1381
TseFI GTSAC 2 cut(s) 1348, 1433
TseI GCWGC 7 cut(s) 98, 209, 557, 867, 1133, 1331, 1481
Tsp45I GTSAC 2 cut(s) 1348, 1433
TspDTI ATGAA 5 cut(s) 17, 97, 259, 939, 1357
TspRI CASTG 3 cut(s) 378, 1102, 1381
VpaK11BI GGWCC 2 cut(s) 466, 686
VspI ATTAAT 1 cut(s) 1106
XagI CCTNNNNNAGG 1 cut(s) 81
XapI RAATTY 2 cut(s) 1049, 1498
XcmI CCANNNNNNNNNTGG 1 cut(s) 1215
XhoI CTCGAG 1 cut(s) 188
XmaJI CCTAGG 1 cut(s) 691
XmnI GAANNNNTTC 1 cut(s) 198
XspI CTAG 2 cut(s) 428, 692
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.