Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0001576

Physical Location & Seq

Genomic Coordinates Reverse (-)

21907295 .. 21909844

Gene Structure 2550 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ23844

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2076 bp

ATGCAAAACTATCACTTTGGTACCCCAAGTTTGAAGCCTGACCCAACATTCATACATGCCGTCAATAACGGCGTTAAGTCTCAAGCCACGTGGCAAACCTTGAGGGGTATTTTGGTCACTCAATGTGTTAACCCAGATTTTTTTTTGTTTCTTTCTCCCTTCTGTTCTTCTCTCCCCCTCCCCTATGTTCTTCTTTATTCTTTACTTCACTCTCTCTCTCTCTCTCTCTCCCCCCCCTCCCCTCTGTTCTTCTTTTCACAGGCGCTGGAGATGATGCGGAGAGCTGCTGCTGCTGCTTCTTCTTCTTATTGCAACAACAGAGGTAGAGGAGGAGGAGGAGGTATGCCTCGTCTTCATTCTATTCTTGTTTTCTTCAACAATATCTTTGTATATTCCTTTCATTATTCTCAAGCTTCAGTGGCCAGCACTATTAGAAACCCACCGAAGCTCACTAATCTTGGGGTTGGGGATGGCGTAAGTTTGTTCAATGAGATGCTTCAAATGCGTCCTCTGCCTTGTGTTGTCGGTTTCAATCAAATCATGGGTCACATCGCAAAATTGAAACATTATCAGGCGGTCATTTCTCTGTATACCCAAATGGATAGGCTAAGAGTTGTTCCCGATGCTTACACTCTCAATATTATCATCAATTGCTTTTGTCATTTGAACCAAATGGGATTCAGTTTGTCTGTGTTGGGAAAATTCTTCAAATTTGGTATTCAACCAAATATCACAACCCTCAACACTCTTATCAAGGGCTTTTTCATTGAAGGGAAAGTGTCTCAAGCAGCACTACTTTTCAGCGCAATGGTGGAGCGAGGTTGTAAGCCGAATGATGTTACTTTCGGAACACTAATAAAGGGCTATTGCATGATGGGAAATAATACTGCAGCTATTCAATTGCTTAGGAAGATGGAACAACAATCTCGAGATTGTAAGCCTAATGTGGTTGTCTATAATATCATCATTGACAGTCTCTGTAAGGACACACTAGTTCCTGATGCATTTAAGCTCTTTTCAGAAATGATTAGTAGGGGCATAGCCCCTGACGTCATTACTTATACCTCTTTGATTCATGGAGTTTGCAAATTAGGCCAGTGGAAAGAAGCTACGAGATTGTTGAATGAGATGGTCAGTAAAGATATCTTCCCGAATTTAGTAACCTTCAGTATCCTGGTCGATACACTTTGTAAGGAGGGGATGGTTGTGGAAGCACAAAGTGTGCTTGAATTGATGATTCAAAGAGGTATCAAACCTGATACAGTTACCTACAGTTCACTTATGGATGGCTACTGTCTGCGAGGAGAAATGGAGAAAGCTAAAGAGGTTTTTGATATGATGAATAGCAAGGGCTCCGTTGTTAATGCTTATAGTTATACCATACTGATAAATGGATATTGTAGGTGTAAAAGGATAAATGAGGCCATCAGGCTTTTTCACGAAATGTCTGATAGGGGACTGGTTCCAAATACCGTCACATATACTGCTCTTATGGATGGTTTTTGCAAAGTGGGAAGAATACTAGATGCCCAGAGATTGTTCAGTAAGTTGCAAGCTTGTGGCCAAGTTCTTGATGTTCAAACTTATGCCGTTCTACTGGATGGCCTGTTTAAAAACCAACAACTTGCTATGGCAATGGAGTTGTTCGGGGAGTTGGAATGCATGAAGCTGGATTTGAATATAGTAATATACAGTACTCTTATTGAAGGTTTGTGTATAGCTGGAAAATTTGAATCCGCAAAGGACCACTTTTACTGTTTATCATCAAAAGGACTTGAACCTGATGCGAGGACGTACACTATAATGATCAAGGGACTTTGTGAAGGAGGCTTAATTAGTGAAGCAGAAAAGTTGCTTAGGGAAATGGAACAGAAAAGCGTTGCTCCTGATGATTGCACATATAACACAATTATCCGAGGGTTTCTCAATAATGACGAGACAGTAAAGGCAGTGGGACTTGTTCACGAAATGATGGACAGGGGTTTCTCTGCAGATGCATGGACGATGGAATTAATCATTGGTTTATTGTCTAAAGATAAAGTAGATCCTGCTTTGTTGTCATTGATAGAAAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000959 GO:0000963 GO:0000966 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004016 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009451 GO:0009791 GO:0009845 GO:0009975 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0016556 GO:0016787 GO:0016788 GO:0016829 GO:0016849 GO:0032501 GO:0032502 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0048856 GO:0071704 GO:0080156 GO:0090304 GO:0090305 GO:0090351 GO:0090615 GO:0097159 GO:0140053 GO:1900864 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

691

Amino Acids

77.01

Weight (kDa)

7.93

Isoelectric Point (pI)

28.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	172 - 221	2.3e-10	PPR repeat family
PPR_3	PF13812	238 - 289	1.4e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	242 - 290	7.3e-15	PPR repeat family
PPR_3	PF13812	267 - 324	2.7e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	273 - 306	7.5e-11	PPR repeat
PPR_2	PF13041	280 - 328	1.3e-10	PPR repeat family
PPR_1	PF12854	310 - 342	5.4e-12	PPR repeat
PPR_2	PF13041	314 - 363	5.5e-19	PPR repeat family
PPR	PF01535	317 - 347	2.5e-06	PPR repeat
PPR_long	PF17177	330 - 429	7.9e-09	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	337 - 396	7.5e-11	Pentatricopeptide repeat domain
PPR_1	PF12854	345 - 378	2.3e-12	PPR repeat
PPR_2	PF13041	349 - 398	2.7e-17	PPR repeat family
PPR	PF01535	352 - 382	1.9e-07	PPR repeat
PPR_1	PF12854	380 - 412	4.5e-09	PPR repeat
PPR_2	PF13041	395 - 432	3.9e-10	PPR repeat family
PPR_1	PF12854	415 - 447	7.2e-12	PPR repeat
PPR_2	PF13041	421 - 468	1.5e-14	PPR repeat family
PPR	PF01535	422 - 451	4.2e-08	PPR repeat
TPR_24	PF23276	436 - 551	1.9e-11	Fungal tetratrico peptide repeats
PPR_3	PF13812	443 - 499	1.1e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	450 - 483	3.4e-11	PPR repeat
PPR	PF01535	457 - 487	9.7e-09	PPR repeat
PPR_2	PF13041	458 - 503	2e-18	PPR repeat family
PPR_1	PF12854	485 - 516	1.4e-10	PPR repeat
PPR_2	PF13041	489 - 538	4.2e-12	PPR repeat family
PPR_2	PF13041	560 - 607	6.5e-13	PPR repeat family
PPR_3	PF13812	589 - 641	2.7e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	590 - 623	1.1e-12	PPR repeat
PPR	PF01535	598 - 626	3.8e-07	PPR repeat
PPR_2	PF13041	606 - 642	4.1e-08	PPR repeat family
PPR_2	PF13041	629 - 674	3.2e-08	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001576)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G49830 AT5G67630
fragaria_vesca	FvH4_1g17590
malus_domestica	MD02G1184700.v1.1 MD08G1213300.v1.1
prunus_persica	Prupe.1G544700_v2.0.a1
pyrus_communis	pycom02g14980 pycom15g25960
rosa_chinensis	RchiOBHm_Chr2g0107901 RchiOBHm_Chr6g0265871 RchiOBHm_Chr6g0265881 RchiOBHm_Chr6g0265891 RchiOBHm_Chr6g0265911
rosa_laevigata	RLG00000014147 RLG00000014151 RLG00000014152 RLG00000017626 RLG00000030880
rosa_multiflora	Rmu_sc0000149.1_g000017 Rmu_sc0001484.1_g000033 Rmu_sc0006952.1_g000010 Rmu_sc0006952.1_g000012 Rmu_sc0006952.1_g000034 Rmu_sc0013320.1_g000004 Rmu_sc0013320.1_g000009
rosa_roxburghii	Rroxscaffold_2G00135810 Rroxscaffold_7G00202230 Rroxscaffold_7G00202270 Rroxscaffold_7G00202280 Rroxscaffold_7G00202290
rosa_rugosa	Rorug02G0148600 Rorug02G0148600 Rorug06G0021100 Rorug06G0021100 Rorug06G0021300
rosa_samantha	Rh2AG197900 Rh2BG210800 Rh2CG202600 Rh2DG205600 Rh6BG142600 Rh6BG142700 Rh6CG139200 Rh6CG139300 Rh6DG126800 Rh6DG126900
rosa_wichuraiana	Rw0G009670 Rw0G009680 Rw2G015700 Rw6G012330 Rw6G012340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1053
Acc65I	GGTACC	1 cut(s)	20
AccB1I	GGYRCC	1 cut(s)	20
AccI	GTMKAC	1 cut(s)	590
AciI	CCGC	3 cut(s)	277, 575, 1737
AclWI	GGATC	1 cut(s)	2039
AcoI	YGGCCR	2 cut(s)	420, 1561
AcsI	RAATTY	4 cut(s)	701, 710, 1153, 1727
AcuI	CTGAAG	2 cut(s)	399, 1150
AcvI	CACGTG	1 cut(s)	90
AcyI	GRCGYC	1 cut(s)	1050
AdeI	CACNNNGTG	1 cut(s)	1220
AfaI	GTAC	3 cut(s)	22, 1696, 1796
AhlI	ACTAGT	1 cut(s)	991
AjnI	CCWGG	1 cut(s)	1173
AjuI	GAANNNNNNNTTGG	2 cut(s)	2001, 2033
Alw26I	GTCTC	4 cut(s)	84, 786, 980, 1931
AlwI	GGATC	1 cut(s)	2039
AlwNI	CAGNNNCTG	2 cut(s)	265, 978
Ama87I	CYCGRG	1 cut(s)	927
AoxI	GGCC	5 cut(s)	420, 1093, 1422, 1561, 1603
ApeKI	GCWGC	6 cut(s)	284, 287, 290, 293, 788, 890
ApoI	RAATTY	4 cut(s)	701, 710, 1153, 1727
ArsI	GACNNNNNNTTYG	2 cut(s)	529, 561
AseI	ATTAAT	1 cut(s)	2012
Asp718I	GGTACC	1 cut(s)	20
AspLEI	GCGC	2 cut(s)	265, 806
AspS9I	GGNCC	1 cut(s)	1744
AvaI	CYCGRG	1 cut(s)	927
AvaII	GGWCC	1 cut(s)	1744
BaeI	ACNNNNGTAYC	1 cut(s)	37
BalI	TGGCCA	2 cut(s)	422, 1563
BanI	GGYRCC	1 cut(s)	20
BanII	GRGCYC	1 cut(s)	1355
BbrPI	CACGTG	1 cut(s)	90
BbsI	GAAGAC	1 cut(s)	344
BbvI	GCAGC	6 cut(s)	271, 274, 277, 280, 800, 902
BceAI	ACGGC	3 cut(s)	44, 85, 1574
BciT130I	CCWGG	1 cut(s)	1175
BciVI	GTATCC	1 cut(s)	1181
BclI	TGATCA	1 cut(s)	1806
BcoDI	GTCTC	4 cut(s)	84, 786, 980, 1931
BcuI	ACTAGT	1 cut(s)	991
BfaI	CTAG	2 cut(s)	992, 1523
BfmI	CTRYAG	3 cut(s)	888, 1270, 1989
BfoI	RGCGCY	1 cut(s)	266
BfuI	GTATCC	1 cut(s)	1181
BisI	GCNGC	6 cut(s)	285, 288, 291, 294, 789, 891
BlsI	GCNGC	6 cut(s)	286, 289, 292, 295, 790, 892
BmcAI	AGTACT	1 cut(s)	1696
Bme1390I	CCNGG	1 cut(s)	1175
Bme18I	GGWCC	1 cut(s)	1744
BmeT110I	CYCGRG	1 cut(s)	927
BmgT120I	GGNCC	1 cut(s)	1744
BmiI	GGNNCC	3 cut(s)	22, 1354, 1464
BmrFI	CCNGG	1 cut(s)	1175
BmsI	GCATC	7 cut(s)	264, 483, 613, 991, 1516, 1774, 1984
BpiI	GAAGAC	1 cut(s)	344
BplI	GAGNNNNNCTC	2 cut(s)	1908, 1940
BpmI	CTGGAG	1 cut(s)	287
Bpu10I	CCTNAGC	2 cut(s)	905, 1856
BpuEI	CTTGAG	4 cut(s)	66, 121, 393, 768
BsaAI	YACGTR	1 cut(s)	90
BsaHI	GRCGYC	1 cut(s)	1050
BsaJI	CCNNGG	1 cut(s)	1915
Bse1I	ACTGG	3 cut(s)	1096, 1464, 1602
Bse3DI	GCAATG	2 cut(s)	813, 1641
BseBI	CCWGG	1 cut(s)	1175
BseDI	CCNNGG	1 cut(s)	1915
BseGI	GGATG	5 cut(s)	475, 1206, 1291, 1501, 1606
BseMI	GCAATG	2 cut(s)	813, 1641
BseNI	ACTGG	3 cut(s)	1096, 1464, 1602
BseRI	GAGGAG	5 cut(s)	342, 345, 348, 351, 1317
BseXI	GCAGC	6 cut(s)	271, 274, 277, 280, 800, 902
BshFI	GGCC	5 cut(s)	422, 1095, 1424, 1563, 1605
BshNI	GGYRCC	1 cut(s)	20
BsiHKCI	CYCGRG	1 cut(s)	927
BslFI	GGGAC	3 cut(s)	1470, 1827, 1968
BsmAI	GTCTC	4 cut(s)	84, 786, 980, 1931
BsmFI	GGGAC	3 cut(s)	1470, 1827, 1968
BsmI	GAATGC	1 cut(s)	1664
BsnI	GGCC	5 cut(s)	422, 1095, 1424, 1563, 1605
BsoBI	CYCGRG	1 cut(s)	927
Bsp1286I	GDGCHC	1 cut(s)	1355
Bsp143I	GATC	2 cut(s)	1806, 2044
BspACI	CCGC	3 cut(s)	277, 575, 1737
BspANI	GGCC	5 cut(s)	422, 1095, 1424, 1563, 1605
BspLI	GGNNCC	3 cut(s)	22, 1354, 1464
BspMAI	CTGCAG	2 cut(s)	892, 1993
BspPI	GGATC	1 cut(s)	2039
BspT107I	GGYRCC	1 cut(s)	20
BsrDI	GCAATG	2 cut(s)	813, 1641
BsrI	ACTGG	3 cut(s)	1096, 1464, 1602
BssECI	CCNNGG	1 cut(s)	1915
BssMI	GATC	2 cut(s)	1806, 2044
BssNAI	GTATAC	1 cut(s)	591
BssNI	GRCGYC	1 cut(s)	1050
Bst1107I	GTATAC	1 cut(s)	591
Bst2UI	CCWGG	1 cut(s)	1175
Bst4CI	ACNGT	8 cut(s)	974, 1264, 1274, 1295, 1474, 1694, 1757, 1942
BstACI	GRCGYC	1 cut(s)	1050
BstBAI	YACGTR	1 cut(s)	90
BstC8I	GCNNGC	2 cut(s)	424, 1554
BstDEI	CTNAG	3 cut(s)	608, 905, 1856
BstF5I	GGATG	5 cut(s)	475, 1206, 1291, 1501, 1606
BstH2I	RGCGCY	1 cut(s)	266
BstHHI	GCGC	2 cut(s)	265, 806
BstKTI	GATC	2 cut(s)	1809, 2047
BstMAI	GTCTC	4 cut(s)	84, 786, 980, 1931
BstMBI	GATC	2 cut(s)	1806, 2044
BstMWI	GCNNNNNNNGC	6 cut(s)	290, 293, 419, 502, 511, 1092
BstNI	CCWGG	1 cut(s)	1175
BstNSI	RCATGY	1 cut(s)	59
BstSCI	CCNGG	1 cut(s)	1173
BstSFI	CTRYAG	3 cut(s)	888, 1270, 1989
BstV1I	GCAGC	6 cut(s)	271, 274, 277, 280, 800, 902
BstV2I	GAAGAC	1 cut(s)	344
BstX2I	RGATCY	1 cut(s)	2044
BstYI	RGATCY	1 cut(s)	2044
BstZ17I	GTATAC	1 cut(s)	591
BsuI	GTATCC	1 cut(s)	1181
BsuRI	GGCC	5 cut(s)	422, 1095, 1424, 1563, 1605
BtgZI	GCGATG	1 cut(s)	535
BtsCI	GGATG	5 cut(s)	475, 1206, 1291, 1501, 1606
BtsI	GCAGTG	1 cut(s)	1956
BtsIMutI	CAGTG	3 cut(s)	423, 1103, 1956
Cac8I	GCNNGC	2 cut(s)	424, 1554
CaiI	CAGNNNCTG	2 cut(s)	265, 978
CfoI	GCGC	2 cut(s)	265, 806
Cfr13I	GGNCC	1 cut(s)	1744
CseI	GACGC	1 cut(s)	494
Csp6I	GTAC	3 cut(s)	21, 1695, 1795
CspCI	CAANNNNNGTGG	2 cut(s)	71, 106
CviAII	CATG	6 cut(s)	56, 541, 871, 1076, 1663, 1998
CviQI	GTAC	3 cut(s)	21, 1695, 1795
DdeI	CTNAG	3 cut(s)	608, 905, 1856
DpnI	GATC	2 cut(s)	1808, 2046
DpnII	GATC	2 cut(s)	1806, 2044
DraI	TTTAAA	1 cut(s)	1612
DraIII	CACNNNGTG	1 cut(s)	1220
EaeI	YGGCCR	2 cut(s)	420, 1561
Eco24I	GRGCYC	1 cut(s)	1355
Eco32I	GATATC	1 cut(s)	1144
Eco47I	GGWCC	1 cut(s)	1744
Eco57I	CTGAAG	2 cut(s)	399, 1150
Eco72I	CACGTG	1 cut(s)	90
Eco88I	CYCGRG	1 cut(s)	927
EcoRII	CCWGG	1 cut(s)	1173
EcoRV	GATATC	1 cut(s)	1144
EcoT22I	ATGCAT	3 cut(s)	1006, 1664, 1999
EcoT38I	GRGCYC	1 cut(s)	1355
FaeI	CATG	6 cut(s)	59, 544, 874, 1079, 1666, 2001
FalI	AAGNNNNNCTT	2 cut(s)	1733, 1765
FaqI	GGGAC	3 cut(s)	1470, 1827, 1968
FatI	CATG	6 cut(s)	55, 540, 870, 1075, 1662, 1997
FbaI	TGATCA	1 cut(s)	1806
FblI	GTMKAC	1 cut(s)	590
Fnu4HI	GCNGC	6 cut(s)	285, 288, 291, 294, 789, 891
FokI	GGATG	5 cut(s)	482, 1213, 1298, 1508, 1613
FriOI	GRGCYC	1 cut(s)	1355
Fsp4HI	GCNGC	6 cut(s)	285, 288, 291, 294, 789, 891
FspBI	CTAG	2 cut(s)	992, 1523
GlaI	GCGC	2 cut(s)	264, 805
GluI	GCNGC	6 cut(s)	285, 288, 291, 294, 789, 891
GsuI	CTGGAG	1 cut(s)	287
HaeII	RGCGCY	1 cut(s)	266
HaeIII	GGCC	5 cut(s)	422, 1095, 1424, 1563, 1605
HgaI	GACGC	1 cut(s)	494
HhaI	GCGC	2 cut(s)	265, 806
Hin1I	GRCGYC	1 cut(s)	1050
Hin1II	CATG	6 cut(s)	59, 544, 874, 1079, 1666, 2001
Hin6I	GCGC	2 cut(s)	263, 804
HinP1I	GCGC	2 cut(s)	263, 804
HincII	GTYRAC	1 cut(s)	130
HindII	GTYRAC	1 cut(s)	130
HindIII	AAGCTT	2 cut(s)	411, 1554
HinfI	GANTC	4 cut(s)	678, 1072, 1237, 1733
HpaI	GTTAAC	1 cut(s)	130
Hpy166II	GTNNAC	5 cut(s)	130, 591, 1277, 1797, 1963
Hpy188I	TCNGA	4 cut(s)	848, 1021, 1450, 1916
Hpy188III	TCNNGA	9 cut(s)	620, 927, 929, 998, 1150, 1439, 1571, 1886, 1964
Hpy8I	GTNNAC	5 cut(s)	130, 591, 1277, 1797, 1963
HpyAV	CCTTC	5 cut(s)	169, 764, 1174, 1700, 1817
HpyCH4III	ACNGT	8 cut(s)	974, 1264, 1274, 1295, 1474, 1694, 1757, 1942
HpyCH4IV	ACGT	3 cut(s)	89, 1050, 1793
HpyF10VI	GCNNNNNNNGC	6 cut(s)	290, 293, 419, 502, 511, 1092
HpyF3I	CTNAG	3 cut(s)	608, 905, 1856
HpySE526I	ACGT	3 cut(s)	89, 1050, 1793
Hsp92I	GRCGYC	1 cut(s)	1050
Hsp92II	CATG	6 cut(s)	59, 544, 874, 1079, 1666, 2001
HspAI	GCGC	2 cut(s)	263, 804
KpnI	GGTACC	1 cut(s)	24
Ksp22I	TGATCA	1 cut(s)	1806
KspAI	GTTAAC	1 cut(s)	130
Kzo9I	GATC	2 cut(s)	1806, 2044
LmnI	GCTCC	3 cut(s)	814, 1358, 1888
Lsp1109I	GCAGC	6 cut(s)	271, 274, 277, 280, 800, 902
LweI	GCATC	7 cut(s)	264, 483, 613, 991, 1516, 1774, 1984
MaeI	CTAG	2 cut(s)	992, 1523
MaeII	ACGT	3 cut(s)	89, 1050, 1793
MaeIII	GTNAC	6 cut(s)	115, 545, 838, 1159, 1264, 1474
MalI	GATC	2 cut(s)	1808, 2046
MboI	GATC	2 cut(s)	1806, 2044
MfeI	CAATTG	2 cut(s)	649, 899
MflI	RGATCY	1 cut(s)	2044
MhlI	GDGCHC	1 cut(s)	1355
MlsI	TGGCCA	2 cut(s)	422, 1563
MluNI	TGGCCA	2 cut(s)	422, 1563
MmeI	TCCRAC	1 cut(s)	1635
Mox20I	TGGCCA	2 cut(s)	422, 1563
Mph1103I	ATGCAT	3 cut(s)	1006, 1664, 1999
MscI	TGGCCA	2 cut(s)	422, 1563
MseI	TTAA	7 cut(s)	75, 129, 1008, 1362, 1611, 1832, 2012
MslI	CAYNNNNRTG	1 cut(s)	1802
Msp20I	TGGCCA	2 cut(s)	422, 1563
MspR9I	CCNGG	1 cut(s)	1175
MunI	CAATTG	2 cut(s)	649, 899
Mva1269I	GAATGC	1 cut(s)	1664
MvaI	CCWGG	1 cut(s)	1175
MwoI	GCNNNNNNNGC	6 cut(s)	290, 293, 419, 502, 511, 1092
NdeII	GATC	2 cut(s)	1806, 2044
NlaIII	CATG	6 cut(s)	59, 544, 874, 1079, 1666, 2001
NlaIV	GGNNCC	3 cut(s)	22, 1354, 1464
NmuCI	GTSAC	3 cut(s)	115, 545, 1474
NsiI	ATGCAT	3 cut(s)	1006, 1664, 1999
NspI	RCATGY	1 cut(s)	59
PaeR7I	CTCGAG	1 cut(s)	927
PctI	GAATGC	1 cut(s)	1664
PfeI	GAWTC	4 cut(s)	678, 1072, 1237, 1733
PkrI	GCNGC	6 cut(s)	286, 289, 292, 295, 790, 892
PmaCI	CACGTG	1 cut(s)	90
PmlI	CACGTG	1 cut(s)	90
Ppu21I	YACGTR	1 cut(s)	90
PshBI	ATTAAT	1 cut(s)	2012
Psp6I	CCWGG	1 cut(s)	1173
PspCI	CACGTG	1 cut(s)	90
PspGI	CCWGG	1 cut(s)	1173
PspN4I	GGNNCC	3 cut(s)	22, 1354, 1464
PspPI	GGNCC	1 cut(s)	1744
PstI	CTGCAG	2 cut(s)	892, 1993
PstNI	CAGNNNCTG	2 cut(s)	265, 978
PsuI	RGATCY	1 cut(s)	2044
RsaI	GTAC	3 cut(s)	22, 1696, 1796
RsaNI	GTAC	3 cut(s)	21, 1695, 1795
RseI	CAYNNNNRTG	1 cut(s)	1802
SaqAI	TTAA	7 cut(s)	75, 129, 1008, 1362, 1611, 1832, 2012
SatI	GCNGC	6 cut(s)	285, 288, 291, 294, 789, 891
Sau3AI	GATC	2 cut(s)	1806, 2044
Sau96I	GGNCC	1 cut(s)	1744
ScaI	AGTACT	1 cut(s)	1696
ScrFI	CCNGG	1 cut(s)	1175
SduI	GDGCHC	1 cut(s)	1355
SfaNI	GCATC	7 cut(s)	264, 483, 613, 991, 1516, 1774, 1984
SfcI	CTRYAG	3 cut(s)	888, 1270, 1989
Sfr274I	CTCGAG	1 cut(s)	927
SinI	GGWCC	1 cut(s)	1744
SlaI	CTCGAG	1 cut(s)	927
SmiMI	CAYNNNNRTG	1 cut(s)	1802
SmlI	CTYRAG	5 cut(s)	81, 100, 408, 783, 927
SmoI	CTYRAG	5 cut(s)	81, 100, 408, 783, 927
SpeI	ACTAGT	1 cut(s)	991
SsiI	CCGC	3 cut(s)	277, 575, 1737
SspI	AATATT	1 cut(s)	640
SspMI	CTAG	2 cut(s)	992, 1523
StyD4I	CCNGG	1 cut(s)	1173
TaaI	ACNGT	8 cut(s)	974, 1264, 1274, 1295, 1474, 1694, 1757, 1942
TaiI	ACGT	3 cut(s)	92, 1053, 1796
TaqI	TCGA	2 cut(s)	928, 1179
TatI	WGTACW	1 cut(s)	1694
TfiI	GAWTC	4 cut(s)	678, 1072, 1237, 1733
Tru1I	TTAA	7 cut(s)	75, 129, 1008, 1362, 1611, 1832, 2012
Tru9I	TTAA	7 cut(s)	75, 129, 1008, 1362, 1611, 1832, 2012
TscAI	CASTG	3 cut(s)	423, 1103, 1956
TseFI	GTSAC	3 cut(s)	115, 545, 1474
TseI	GCWGC	6 cut(s)	284, 287, 290, 293, 788, 890
Tsp45I	GTSAC	3 cut(s)	115, 545, 1474
TspDTI	ATGAA	7 cut(s)	40, 344, 389, 754, 1064, 1355, 1679
TspGWI	ACGGA	1 cut(s)	1345
TspRI	CASTG	3 cut(s)	423, 1103, 1956
VpaK11BI	GGWCC	1 cut(s)	1744
VspI	ATTAAT	1 cut(s)	2012
XapI	RAATTY	4 cut(s)	701, 710, 1153, 1727
XceI	RCATGY	1 cut(s)	59
XhoI	CTCGAG	1 cut(s)	927
XmiI	GTMKAC	1 cut(s)	590
XspI	CTAG	2 cut(s)	992, 1523
ZraI	GACGTC	1 cut(s)	1051
ZrmI	AGTACT	1 cut(s)	1696
Zsp2I	ATGCAT	3 cut(s)	1006, 1664, 1999

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.