Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0001315

Physical Location & Seq

Genomic Coordinates Forward (+)

37211615 .. 37226789

Gene Structure 15175 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000002625

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3972 bp

ATGGCCTCTCTACCTTTCTCGAAGAAGTACCGGTGCGTTCCTTCTCTGCGTCCATTCTACTCCGGCAGCGCCTTTGCAGTCTCGTCCGACGGCTCCTTCCTGGCGTGCGCGTCCGGCGAGTCCATCAACATTGTTAATACGTCCAACTCCCTTATACGGTCCACATTGGAAGGCGACACGGAGGCGTTCACAGCTTTAACACTAAGCCCTGACAACAAGTTGCTATTCTCGGCTAGCCACACTCTACAGATTAGGGTCTGGGAGCTCTCCACATGTAAATGCCTCTTCTCTTGGAAGGCTCATGATCAGCCTGTGATGGCAATGGTGTGTCACCCATCTGGGAAGTATTTTGCCACTGGTGCAGCAGATAAGAAGGTCATAGTTTGGAAGCCAACTTGTGGTTTTTCTCCTGTTTGCTTTACAGGTCCAGGTCATGAAGGAGTTGTTACTACTCTTCTATTTCCTCCAGATCCTACTGAAAAACTTCTTGACATCTTTGTTCATATTTCTTCCGTATTCTGTGAGTGTGATGTAAGGGTTGTAATATTGTGGAACCTTGATGACTATAGCTGCTTGAAGACTGTAACAACGGATGAGGTCCTTGAAGCTGTGTGTGTAATTCCTTCTACGACTCTGTTATATTCCCATTTAGGTTCATCCAAGCTTCACAATGGGAAGAAAAGCAAGTTACATGTAACATATTTCATAACAGTTGGTGACCGTGGCATCATACGAATATGGAATTCCGAACGTGCAGTTTGCCTATTTGAGCAGAAGTGCTCAGATGTTGCTATCACCTCAGATGACGTTGAATCGAAAAGGGGCTTTACTGCAGCATCCATGCTACCATTAGATCTAGGTCTGCTCTGTGTGACAGCTGATCAGGAGTTCCTTTTTTATTCTCTAATTGGAAGCCGTGAAGGAACCGTTGAATTTTCTCTAAGAAAAAGACTCGTCGGAAACAATGGAGAGATTCTGGATATGAAGTTTCTAGGTGATCAGGAACAATTTATTGCTGTTGCCACAAGTTCTGAACAGGTACGAGTGTTTGATGTTGCATCCATGTCATGTTCTCGTGTGTTGGCTGGTCATACGGAAACTGTTATGTGCCTTGACACGTGCGTGTCAAATTCTGGGAGAACACTTATAGCAACTGGAAGCAAGGATAGAAGTGTCAGGTTGTGGGATTTGGATACCAGCTGCATCAGTCTTTGCTTAGCTCACAGTGGTTTTGTTGGAGCTATTGCTTTCTCACAGAAGCAGCGGGATTTCTTTGTTAGTGGTAGTAGTGATGGTACCCTCATGGTCTGGAGTTTCGATTGTCTTTCAGACAATGTGGAAAAGCCGATGAAGTTGAAAGAAAAAGCTGTTGTACAAGCGCACTTTAAGGATATCAATTCAGTAGCTGTTGCACCAAAAGATAGTTTAGTTTGTAGTGGTTCACAGGATCACACTGCTCGTGTATGGAAGCTTCCAGATCTTGTACCAGTAGTTGTACTTAAGGGTCATAAAAGAGCGGTTTGGTGTGTAGAGTTTTCTCCAGTTGAACAATGTGTTCTAACTGCTTCTGGTGATAAAACAATAAAGATATGGGCTATATCGGATGGCTCGTGCTTAAAAACAGTTGAAGGACACATGTCATGTGTATATCGAGCATCATTTCTTAATCATGGGGTCCAATTTATATCTTCAGATGCTGATGGTTTGGTAAAGCTGTGGATGGTTTATACAGAGGAATGCATTGCTACATATGATCAGCATGAGGATAAGATTTCAGCATTGACTGTGGGAAAAAAATCAGAAATGATTGCAACTGGCAGCGGGGATGCTGCCATCAATCTATGGTGCGATCGCACTGCCTGGTCCATAGATCTTAAGACATTAAACAAGGCAGAGAAGAGCACCTGGGATATGACTTTTGTTGTATGGCCAAATTACGTCCTCATGCTTGCAGAGTGGCCATTTGGAGGCACATTTCTTACAGCTAAAGAGGTCTTTGACTTTCAAGCATGCAGCAAAGGGACAACATTAATTTTTTCATATAAAAATAATAGTTTTGTTCTCGGGGCGGAGTCACGGGAGACGTTTGAAGCTAAACCAAAGACATATATCGCGGGTGAGCATAGGCAGAAGTTTGAAGTGTTTGGCGACCAAACATGCGTGGCTTATACTGGGCATGTCCAAATTGGTAAGGAGTTTATTGACCATGTTCGTGGAAAGCTCAAGGGCGTTAAGCGGAAAGTGACTCTCCCTGAAGTTGTTGCATTAGGATATGATTTTCATCCCACAAGTGAAGGCAAAGCTGATATATTTATCGGAGGATTGCTGAAAAATGGGGTACCCCGTATGCATCGATTTTTAATCGATTACAGTACCAAAAAATCTGAAATGAAGAAGACATGCCTAATTTTCAATTTACCTGAGGGTTGCTTTGCAACGGGTTCTGGACGGAAAGATGCAGAAAATTTCTTTGCCGGTGCCAGGAACACTTCATTGGGTCAGAAACAGTTTGTCAAAAGGGGCATGATTAAAGCGTGTATTGAAAATGAGATGAGTGGAGGAGTTGTTTCAATCATTCATCTTCAAACAAGTAGAGCAAGTGAGGTATCTCAATGCAAGACTATTGATGGGCTGGTTGAAGATTTCCATTACTTTGATTCCAAGAGTAGGGCAATCATAATGTACAACAAGCAAACACAAGACAACTCATATTTTCTAAGCTGGAGGAAGAGGGTGATACCTAAAATGAGGTTTCACATATGTTCACCACTTGCGCAGCTTGCTGACAGCAATCTTGTCGTGGCCCTGGATAAGCTCAAGGATAAGTTAGCTGAGTATGATCTAATCCCTGATAAATTCAAGCTCTCTGTTCTGTTACCAATCCCCCATCAAATTTCACCTCCAAAACAAGTTGACCCCACTGATTCGAATAAGAAGGTAGAATCTGTTAGGGCTGTGGATGGGTTTTTAATGCCTGACGAGAAATTACGTGGGGTTTTTCTTCAGAAATTGAAGGGTGTAGCTGTAATTGAGCATGCGCTCGACAATGTTGGTGTGGATTTGAGTGTTGATGTTGTTGCACAAGTAGTGAATAGAGGTTGTTTAGGCGGTGAAGCAATGCTTGTGTTTTTTAATTGGGCGATTAAGAAGCCAAATGTAGCTAAGCATGTTCATACATACCATATAATCCTCAAAGCATTGGGTAGAAGAAAGTTCTTTAATTATATGATGCAAGTTTTGAATGACATGAGGAGCCGAGACATACGTCTCGATTTGGAGACTGTGACGATTGTGATGGATAGTTTTGTGAGGGCTGGACATGTCTCGAAAGCAATCCAAGTGTTCCGAAACTCGGCAGAGTTCGGGTTGGATTGCGATACTGAGTGTCTGAATGTGCTTCTGCAGTGTCTGTGTCGGCGGTCTCATGTAGTGGCTGCAAATTCGTTTCTTAATTCAGTGAAGGAGAAAGTGCGATTCAATGGCACTACGTATAACATTGTTATTGGTGGGTGGTCGAAATATGGTAGAGTTGGTGAGATGGAGAGGACTTTGGAAGCAATGGTAGCAGATGGGTTTAGTCCTGATAGTGCAACTTTCAGTTACATTCTTGAGGGTCTAGGAAGAGCTGATCGGATTGACGATGCAGTTGAGATTTTTGAGAGCATGAAGGGGAAAGGTTGCGTGCCAGACACCATTACATACAATGCAATGATCTCTAACTTTATCTCTGTTGCGAATATTGATGAATGTGTAAAGTACTTCAAGAGTATGTCGAGTAATGCTTGCGATCCAAATGTTGATACTTATACCAAATTGATTGCTGGTTTCCTTAAAGCCCGGAAAGTGGCGGATGCACTGGAGATGTTTGATGAGATGTTAAGTCAAGGATTTGTTCCTACTACGGGGACTATAACCTCTTTTATGGAACCCCTATGTAGCTATGGTCCACCATATGCTGCTATGATGATCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000462 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0022613 GO:0030490 GO:0030515 GO:0030684 GO:0030686 GO:0031974 GO:0031981 GO:0032040 GO:0032991 GO:0034388 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042274 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0070013 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03008 map03008

Pfam Domains

Protein Families

Utp13 WD40

Protein Analysis

1324

Amino Acids

146.57

Weight (kDa)

7.27

Isoelectric Point (pI)

33.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR5	PF25175	23 - 217	5.2e-18	WDR5 beta-propeller domain
WD40_WDHD1_1st	PF24817	24 - 100	9.2e-08	WDHD1 first WD40 domain
WD40_CDC20-Fz	PF24807	25 - 220	2.3e-11	CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	25 - 138	4.9e-07	WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3	PF25174	25 - 213	6.4e-16	THOC3 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	25 - 130	1e-10	WDR3 second beta-propeller domain
Beta-prop_WDR75_1st	PF23869	27 - 89	2.2e-06	WD repeat-containing protein 75 first beta-propeller
WD40_Prp19	PF24814	31 - 130	4.2e-13	Prp19 WD40 domain
Beta-prop_WDR3_1st	PF25173	33 - 215	1e-16	WDR3 first beta-propeller domain
WD40	PF00400	93 - 129	4.8e-07	WD domain, G-beta repeat
WD40_Prp19	PF24814	235 - 397	2.8e-07	Prp19 WD40 domain
Beta-prop_WDR3_2nd	PF25172	286 - 490	2.6e-17	WDR3 second beta-propeller domain
WD40_MABP1-WDR62_2nd	PF24782	286 - 438	2e-12	MABP1/WDR62 second WD40 domain
WD40_Gbeta	PF25391	297 - 510	5.9e-10	G protein beta WD-40 repeat protein
Beta-prop_WDR5	PF25175	311 - 439	3.7e-19	WDR5 beta-propeller domain
Beta-prop_THOC3	PF25174	320 - 440	1.7e-14	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	320 - 439	1.3e-15	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	323 - 439	1e-10	Prp19 WD40 domain
WDR55	PF24796	326 - 437	6.7e-06	WDR55
WD40_CDC20-Fz	PF24807	328 - 444	4.1e-13	CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	333 - 429	3.4e-08	WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st	PF24817	335 - 440	1.6e-11	WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_1st	PF25171	337 - 440	5.5e-06	WDR36/Utp21 first beta-propeller
Beta-prop_TEP1_2nd	PF25047	349 - 439	7.5e-09	TEP-1 second beta-propeller
WD40	PF00400	356 - 396	1e-07	WD domain, G-beta repeat
WD40_MABP1-WDR62_2nd	PF24782	363 - 574	2.3e-17	MABP1/WDR62 second WD40 domain
Beta-prop_EML_2	PF23414	381 - 513	2e-10	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_2nd	PF25172	402 - 616	4.6e-29	WDR3 second beta-propeller domain
Beta-prop_THOC3	PF25174	408 - 640	6.2e-27	THOC3 beta-propeller domain
WD40_Prp19	PF24814	415 - 621	9.5e-28	Prp19 WD40 domain
Beta-prop_CAF1B_HIR1	PF24105	417 - 540	6.9e-10	CAF1B/HIR1 beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	425 - 616	1.5e-12	TEP-1 second beta-propeller
WD40_MABP1-WDR62_1st	PF24780	447 - 575	4.8e-06	MABP1/WDR62 first WD40 domain
WD40_CDC20-Fz	PF24807	454 - 615	4.3e-21	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st	PF25173	455 - 615	7e-32	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	458 - 643	1.3e-33	WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	459 - 632	1.8e-08	WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st	PF24817	460 - 548	8.7e-14	WDHD1 first WD40 domain
EIF3I	PF24805	460 - 553	3.2e-10	EIF3I
WDR55	PF24796	464 - 615	6.7e-15	WDR55
WDR90_beta-prop_4th	PF23342	465 - 599	1.9e-06	WDR90, 4th beta-propeller
Beta-prop_EML	PF23409	481 - 617	4e-09	Echinoderm microtubule-associated protein first beta-propeller
WD40	PF00400	496 - 531	2.3e-08	WD domain, G-beta repeat
WD40_Gbeta	PF25391	500 - 618	1.5e-12	G protein beta WD-40 repeat protein
Beta-prop_EML_2	PF23414	507 - 618	9.9e-08	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_SCAP	PF24017	519 - 619	7.6e-06	SCAP Beta-propeller
TPR_24	PF23276	1129 - 1257	9.4e-06	Fungal tetratrico peptide repeats
PPR_2	PF13041	1163 - 1207	1.8e-07	PPR repeat family
PPR_3	PF13812	1184 - 1240	2e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	1190 - 1223	8.5e-06	PPR repeat
PPR_2	PF13041	1202 - 1241	6e-09	PPR repeat family
PPR_long	PF17177	1210 - 1307	1.5e-07	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	1229 - 1278	6.6e-11	PPR repeat family
PPR_1	PF12854	1262 - 1292	3.3e-06	PPR repeat
PPR_2	PF13041	1264 - 1312	1.6e-09	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001315)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G43820
fragaria_vesca	FvH4_5g24880 FvH4_5g25350 FvH4_5g25520
malus_domestica	MD16G1284000.v1.1
prunus_persica	Prupe.5G009500_v2.0.a1
pyrus_communis	pycom16g26010
rosa_chinensis	RchiOBHm_Chr6g0271861 RchiOBHm_Chr6g0271871 RchiOBHm_Chr6g0271981 RchiOBHm_Chr7g0215761 RchiOBHm_Chr7g0215771 RchiOBHm_Chr7g0215901 RchiOBHm_Chr7g0217231 RchiOBHm_Chr7g0219831
rosa_laevigata	RLG00000002611 RLG00000002625 RLG00000013566
rosa_multiflora	Rmu_sc0000055.1_g000008 Rmu_sc0000055.1_g000009 Rmu_sc0000516.1_g000005 Rmu_sc0000516.1_g000006 Rmu_sc0004379.1_g000016 Rmu_sc0006240.1_g000009 Rmu_sc0007857.1_g000006 Rmu_sc0016364.1_g000002 Rmu_sc0016407.1_g000001 Rmu_sc0023052.1_g000001
rosa_roxburghii	Rroxscaffold_2G00114980 Rroxscaffold_3G00243850 Rroxscaffold_5G00342010 Rroxscaffold_7G00186190
rosa_rugosa	Rorug06G0064100 Rorug07G0156600 Rorug07G0157900 Rorug07G0158000
rosa_samantha	Rh1BG116200 Rh6AG181800 Rh6BG185400 Rh6CG182900 Rh6CG183200 Rh6DG174300 Rh6DG174600 Rh7AG294600 Rh7AG306400 Rh7AG306500 Rh7BG286400 Rh7CG314600 Rh7CG325500 Rh7DG296900 Rh7DG299400 Rh7DG306200
rosa_wichuraiana	Rw6G015640 Rw7G026030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	2775
Acc65I	GGTACC	2 cut(s)	1295, 2338
AccB1I	GGYRCC	3 cut(s)	1295, 2338, 2477
AccB7I	CCANNNNNTGG	1 cut(s)	398
AccBSI	CCGCTC	1 cut(s)	1517
AccII	CGCG	2 cut(s)	110, 2114
AciI	CCGC	9 cut(s)	1264, 1517, 1821, 2069, 2114, 2236, 3108, 3418, 3848
AclWI	GGATC	3 cut(s)	464, 1455, 3782
AcoI	YGGCCR	2 cut(s)	1928, 1958
AcsI	RAATTY	7 cut(s)	742, 932, 1129, 2464, 2855, 2892, 3439
AcuI	CTGAAG	3 cut(s)	1674, 2274, 2987
AcvI	CACGTG	1 cut(s)	1119
AfiI	CCNNNNNNNGG	8 cut(s)	156, 398, 1967, 2481, 2667, 3352, 3844, 3902
AflII	CTTAAG	2 cut(s)	1499, 1874
AflIII	ACRYGT	5 cut(s)	272, 691, 1116, 1635, 3319
AgeI	ACCGGT	1 cut(s)	30
AjnI	CCWGG	6 cut(s)	99, 427, 1859, 1904, 2480, 2805
AjuI	GAANNNNNNNTTGG	2 cut(s)	2477, 2509
AleI	CACNNNNGTG	1 cut(s)	1121
Alw21I	GWGCWC	3 cut(s)	267, 782, 1904
Alw26I	GTCTC	7 cut(s)	85, 2075, 3252, 3272, 3272, 3328, 3426
AlwI	GGATC	3 cut(s)	464, 1455, 3782
AlwNI	CAGNNNCTG	3 cut(s)	1406, 1697, 3409
Ama87I	CYCGRG	1 cut(s)	2063
AoxI	GGCC	4 cut(s)	3, 1928, 1958, 2802
ApoI	RAATTY	7 cut(s)	742, 932, 1129, 2464, 2855, 2892, 3439
AseI	ATTAAT	1 cut(s)	2030
AsiGI	ACCGGT	1 cut(s)	30
AsiSI	GCGATCGC	1 cut(s)	1852
Asp700I	GAANNNNTTC	1 cut(s)	483
Asp718I	GGTACC	2 cut(s)	1295, 2338
AspLEI	GCGC	5 cut(s)	71, 110, 1381, 2776, 3040
AspS9I	GGNCC	7 cut(s)	159, 425, 598, 1675, 1863, 2803, 3944
AsuC2I	CCSGG	1 cut(s)	3838
AsuII	TTCGAA	1 cut(s)	2927
AsuNHI	GCTAGC	1 cut(s)	233
AvaI	CYCGRG	1 cut(s)	2063
AvaII	GGWCC	6 cut(s)	159, 425, 598, 1675, 1863, 3944
AxyI	CCTNAGG	1 cut(s)	2421
BaeI	ACNNNNGTAYC	2 cut(s)	1031, 1064
BalI	TGGCCA	2 cut(s)	1930, 1960
BanI	GGYRCC	3 cut(s)	1295, 2338, 2477
BanII	GRGCYC	1 cut(s)	267
BarI	GAAGNNNNNNTAC	2 cut(s)	1455, 1487
BauI	CACGAG	3 cut(s)	1074, 1458, 1609
BbrPI	CACGTG	1 cut(s)	1119
BbsI	GAAGAC	2 cut(s)	584, 2402
Bbv12I	GWGCWC	3 cut(s)	267, 782, 1904
BceAI	ACGGC	2 cut(s)	106, 900
BcgI	CGANNNNNNTGC	4 cut(s)	1838, 1872, 2779, 2813
BciT130I	CCWGG	6 cut(s)	101, 429, 1861, 1906, 2482, 2807
BciVI	GTATCC	1 cut(s)	1186
BclI	TGATCA	4 cut(s)	304, 880, 997, 1753
BcnI	CCSGG	1 cut(s)	3838
BcoDI	GTCTC	7 cut(s)	85, 2075, 3252, 3272, 3272, 3328, 3426
BfaI	CTAG	4 cut(s)	234, 857, 992, 3617
BfmI	CTRYAG	4 cut(s)	245, 565, 831, 3401
BfoI	RGCGCY	1 cut(s)	72
BfrI	CTTAAG	2 cut(s)	1499, 1874
BfuI	GTATCC	1 cut(s)	1186
BglII	AGATCT	3 cut(s)	853, 1477, 1870
BlpI	GCTNAGC	2 cut(s)	1216, 3162
BmcAI	AGTACT	1 cut(s)	3758
Bme1390I	CCNGG	7 cut(s)	101, 429, 1861, 1906, 2482, 2807, 3838
Bme18I	GGWCC	6 cut(s)	159, 425, 598, 1675, 1863, 3944
BmeT110I	CYCGRG	1 cut(s)	2063
BmgT120I	GGNCC	7 cut(s)	159, 425, 598, 1675, 1863, 2803, 3944
BmiI	GGNNCC	9 cut(s)	94, 554, 925, 1297, 1676, 2340, 2479, 3254, 3927
BmrFI	CCNGG	7 cut(s)	101, 429, 1861, 1906, 2482, 2807, 3838
BmrI	ACTGGG	1 cut(s)	2181
BmtI	GCTAGC	1 cut(s)	237
BmuI	ACTGGG	1 cut(s)	2181
BoxI	GACNNNNGTC	2 cut(s)	1636, 3264
BpiI	GAAGAC	2 cut(s)	584, 2402
BpmI	CTGGAG	5 cut(s)	450, 1330, 1524, 2743, 3878
Bpu1102I	GCTNAGC	2 cut(s)	1216, 3162
Bpu14I	TTCGAA	1 cut(s)	2927
BpuEI	CTTGAG	3 cut(s)	2207, 2801, 3629
BpuMI	CCSGG	1 cut(s)	3838
Bsa29I	ATCGAT	2 cut(s)	2353, 2364
BsaAI	YACGTR	3 cut(s)	1119, 2990, 3489
BsaBI	GATNNNNATC	1 cut(s)	2280
BsaI	GGTCTC	1 cut(s)	3426
BsaJI	CCNNGG	3 cut(s)	721, 1905, 2805
BsaWI	WCCGGW	1 cut(s)	30
BsaXI	ACNNNNNCTCC	2 cut(s)	2550, 2580
Bsc4I	CCNNNNNNNGG	8 cut(s)	156, 398, 1967, 2481, 2667, 3352, 3844, 3902
Bse118I	RCCGGY	2 cut(s)	30, 2474
Bse1I	ACTGG	7 cut(s)	361, 1159, 1487, 1541, 1819, 2176, 3861
Bse21I	CCTNAGG	1 cut(s)	2421
Bse3DI	GCAATG	5 cut(s)	327, 1740, 3123, 3564, 3714
Bse8I	GATNNNNATC	1 cut(s)	2280
BseBI	CCWGG	6 cut(s)	101, 429, 1861, 1906, 2482, 2807
BseCI	ATCGAT	2 cut(s)	2353, 2364
BseDI	CCNNGG	3 cut(s)	721, 1905, 2805
BseJI	GATNNNNATC	1 cut(s)	2280
BseLI	CCNNNNNNNGG	8 cut(s)	156, 398, 1967, 2481, 2667, 3352, 3844, 3902
BseMI	GCAATG	5 cut(s)	327, 1740, 3123, 3564, 3714
BseMII	CTCAG	5 cut(s)	795, 813, 2412, 2823, 3372
BseNI	ACTGG	7 cut(s)	361, 1159, 1487, 1541, 1819, 2176, 3861
BseRI	GAGGAG	2 cut(s)	2574, 3265
BsgI	GTGCAG	2 cut(s)	381, 774
Bsh1236I	CGCG	2 cut(s)	110, 2114
Bsh1285I	CGRYCG	1 cut(s)	1852
BshFI	GGCC	4 cut(s)	5, 1930, 1960, 2804
BshNI	GGYRCC	3 cut(s)	1295, 2338, 2477
BshTI	ACCGGT	1 cut(s)	30
BshVI	ATCGAT	2 cut(s)	2353, 2364
BsiEI	CGRYCG	1 cut(s)	1852
BsiHKAI	GWGCWC	3 cut(s)	267, 782, 1904
BsiHKCI	CYCGRG	1 cut(s)	2063
BsiSI	CCGG	5 cut(s)	31, 63, 114, 2475, 3838
BslFI	GGGAC	2 cut(s)	2035, 3919
BslI	CCNNNNNNNGG	8 cut(s)	156, 398, 1967, 2481, 2667, 3352, 3844, 3902
BsmAI	GTCTC	7 cut(s)	85, 2075, 3252, 3272, 3272, 3328, 3426
BsmBI	CGTCTC	2 cut(s)	2075, 3272
BsmFI	GGGAC	2 cut(s)	2035, 3919
BsmI	GAATGC	1 cut(s)	1742
BsnI	GGCC	4 cut(s)	5, 1930, 1960, 2804
Bso31I	GGTCTC	1 cut(s)	3426
BsoBI	CYCGRG	1 cut(s)	2063
Bsp119I	TTCGAA	1 cut(s)	2927
Bsp1286I	GDGCHC	3 cut(s)	267, 782, 1904
Bsp1407I	TGTACA	2 cut(s)	1372, 2682
Bsp1720I	GCTNAGC	2 cut(s)	1216, 3162
BspACI	CCGC	9 cut(s)	1264, 1517, 1821, 2069, 2114, 2236, 3108, 3418, 3848
BspANI	GGCC	4 cut(s)	5, 1930, 1960, 2804
BspCNI	CTCAG	5 cut(s)	794, 812, 2413, 2824, 3373
BspDI	ATCGAT	2 cut(s)	2353, 2364
BspFNI	CGCG	2 cut(s)	110, 2114
BspHI	TCATGA	2 cut(s)	301, 433
BspLI	GGNNCC	9 cut(s)	94, 554, 925, 1297, 1676, 2340, 2479, 3254, 3927
BspMAI	CTGCAG	2 cut(s)	835, 3405
BspOI	GCTAGC	1 cut(s)	237
BspPI	GGATC	3 cut(s)	464, 1455, 3782
BspQI	GCTCTTC	2 cut(s)	1892, 3616
BspT104I	TTCGAA	1 cut(s)	2927
BspT107I	GGYRCC	3 cut(s)	1295, 2338, 2477
BspTI	CTTAAG	2 cut(s)	1499, 1874
BspTNI	GGTCTC	1 cut(s)	3426
BsrBI	CCGCTC	1 cut(s)	1517
BsrDI	GCAATG	5 cut(s)	327, 1740, 3123, 3564, 3714
BsrFI	RCCGGY	2 cut(s)	30, 2474
BsrGI	TGTACA	2 cut(s)	1372, 2682
BsrI	ACTGG	7 cut(s)	361, 1159, 1487, 1541, 1819, 2176, 3861
BssAI	RCCGGY	2 cut(s)	30, 2474
BssECI	CCNNGG	3 cut(s)	721, 1905, 2805
BssSI	CACGAG	3 cut(s)	1074, 1458, 1609
Bst2BI	CACGAG	3 cut(s)	1074, 1458, 1609
Bst2UI	CCWGG	6 cut(s)	101, 429, 1861, 1906, 2482, 2807
Bst6I	CTCTTC	5 cut(s)	290, 459, 1892, 2723, 3616
BstAFI	CTTAAG	2 cut(s)	1499, 1874
BstAUI	TGTACA	2 cut(s)	1372, 2682
BstBAI	YACGTR	3 cut(s)	1119, 2990, 3489
BstBI	TTCGAA	1 cut(s)	2927
BstC8I	GCNNGC	8 cut(s)	106, 235, 1950, 2011, 2781, 3036, 3683, 3784
BstDSI	CCRYGG	1 cut(s)	721
BstEII	GGTNACC	1 cut(s)	716
BstFNI	CGCG	2 cut(s)	110, 2114
BstH2I	RGCGCY	1 cut(s)	72
BstHHI	GCGC	5 cut(s)	71, 110, 1381, 2776, 3040
BstMAI	GTCTC	7 cut(s)	85, 2075, 3252, 3272, 3272, 3328, 3426
BstMCI	CGRYCG	1 cut(s)	1852
BstMWI	GCNNNNNNNGC	5 cut(s)	114, 191, 359, 2780, 3563
BstNI	CCWGG	6 cut(s)	101, 429, 1861, 1906, 2482, 2807
BstPAI	GACNNNNGTC	2 cut(s)	1636, 3264
BstPI	GGTNACC	1 cut(s)	716
BstSCI	CCNGG	7 cut(s)	99, 427, 1859, 1904, 2480, 2805, 3836
BstSFI	CTRYAG	4 cut(s)	245, 565, 831, 3401
BstSNI	TACGTA	1 cut(s)	3489
BstUI	CGCG	2 cut(s)	110, 2114
BstV2I	GAAGAC	2 cut(s)	584, 2402
BstX2I	RGATCY	4 cut(s)	469, 853, 1477, 1870
BstXI	CCANNNNNNTGG	1 cut(s)	2213
BstYI	RGATCY	4 cut(s)	469, 853, 1477, 1870
Bsu15I	ATCGAT	2 cut(s)	2353, 2364
Bsu36I	CCTNAGG	1 cut(s)	2421
BsuI	GTATCC	1 cut(s)	1186
BsuRI	GGCC	4 cut(s)	5, 1930, 1960, 2804
BsuTUI	ATCGAT	2 cut(s)	2353, 2364
BtgI	CCRYGG	1 cut(s)	721
BtsI	GCAGTG	3 cut(s)	1452, 1854, 3410
BtsIMutI	CAGTG	8 cut(s)	354, 1231, 1452, 1854, 2919, 3410, 3462, 3854
Cac8I	GCNNGC	8 cut(s)	106, 235, 1950, 2011, 2781, 3036, 3683, 3784
CaiI	CAGNNNCTG	3 cut(s)	1406, 1697, 3409
CciI	TCATGA	2 cut(s)	301, 433
CfoI	GCGC	5 cut(s)	71, 110, 1381, 2776, 3040
Cfr10I	RCCGGY	2 cut(s)	30, 2474
Cfr13I	GGNCC	7 cut(s)	159, 425, 598, 1675, 1863, 2803, 3944
ClaI	ATCGAT	2 cut(s)	2353, 2364
CseI	GACGC	2 cut(s)	38, 99
CspAI	ACCGGT	1 cut(s)	30
EaeI	YGGCCR	2 cut(s)	1928, 1958
Eam1104I	CTCTTC	5 cut(s)	290, 459, 1892, 2723, 3616
EarI	CTCTTC	5 cut(s)	290, 459, 1892, 2723, 3616
EciI	GGCGGA	2 cut(s)	2084, 3863
Ecl136II	GAGCTC	1 cut(s)	265
Eco105I	TACGTA	1 cut(s)	3489
Eco24I	GRGCYC	1 cut(s)	267
Eco31I	GGTCTC	1 cut(s)	3426
Eco32I	GATATC	1 cut(s)	1393
Eco47I	GGWCC	6 cut(s)	159, 425, 598, 1675, 1863, 3944
Eco53kI	GAGCTC	1 cut(s)	265
Eco57I	CTGAAG	3 cut(s)	1674, 2274, 2987
Eco72I	CACGTG	1 cut(s)	1119
Eco81I	CCTNAGG	1 cut(s)	2421
Eco88I	CYCGRG	1 cut(s)	2063
Eco91I	GGTNACC	1 cut(s)	716
EcoICRI	GAGCTC	1 cut(s)	265
EcoO109I	RGGNCCY	1 cut(s)	598
EcoO65I	GGTNACC	1 cut(s)	716
EcoRI	GAATTC	1 cut(s)	742
EcoRII	CCWGG	6 cut(s)	99, 427, 1859, 1904, 2480, 2805
EcoRV	GATATC	1 cut(s)	1393
EcoT22I	ATGCAT	2 cut(s)	1742, 2352
EcoT38I	GRGCYC	1 cut(s)	267
Esp3I	CGTCTC	2 cut(s)	2075, 3272
FalI	AAGNNNNNCTT	2 cut(s)	3105, 3137
FaqI	GGGAC	2 cut(s)	2035, 3919
FauI	CCCGC	3 cut(s)	1257, 1814, 2107
FauNDI	CATATG	3 cut(s)	1750, 2759, 3952
FbaI	TGATCA	4 cut(s)	304, 880, 997, 1753
FriOI	GRGCYC	1 cut(s)	267
FspBI	CTAG	4 cut(s)	234, 857, 992, 3617
FspI	TGCGCA	1 cut(s)	2775
GlaI	GCGC	5 cut(s)	70, 109, 1380, 2775, 3039
GsuI	CTGGAG	5 cut(s)	450, 1330, 1524, 2743, 3878
HaeII	RGCGCY	1 cut(s)	72
HaeIII	GGCC	4 cut(s)	5, 1930, 1960, 2804
HapII	CCGG	5 cut(s)	31, 63, 114, 2475, 3838
HgaI	GACGC	2 cut(s)	38, 99
HhaI	GCGC	5 cut(s)	71, 110, 1381, 2776, 3040
Hin6I	GCGC	5 cut(s)	69, 108, 1379, 2774, 3038
HinP1I	GCGC	5 cut(s)	69, 108, 1379, 2774, 3038
HincII	GTYRAC	1 cut(s)	2914
HindII	GTYRAC	1 cut(s)	2914
HindIII	AAGCTT	2 cut(s)	662, 1469
HpaII	CCGG	5 cut(s)	31, 63, 114, 2475, 3838
Hpy166II	GTNNAC	6 cut(s)	162, 189, 1442, 2765, 2914, 3947
Hpy8I	GTNNAC	6 cut(s)	162, 189, 1442, 2765, 2914, 3947
Hpy99I	CGWCG	2 cut(s)	92, 959
HpyCH4IV	ACGT	9 cut(s)	140, 751, 807, 1118, 1938, 2084, 2989, 3265, 3488
HpyF10VI	GCNNNNNNNGC	5 cut(s)	114, 191, 359, 2780, 3563
HpySE526I	ACGT	9 cut(s)	140, 751, 807, 1118, 1938, 2084, 2989, 3265, 3488
HspAI	GCGC	5 cut(s)	69, 108, 1379, 2774, 3038
KpnI	GGTACC	2 cut(s)	1299, 2342
Ksp22I	TGATCA	4 cut(s)	304, 880, 997, 1753
LguI	GCTCTTC	2 cut(s)	1892, 3616
LmnI	GCTCC	4 cut(s)	98, 262, 1238, 3252
MaeI	CTAG	4 cut(s)	234, 857, 992, 3617
MaeII	ACGT	9 cut(s)	140, 751, 807, 1118, 1938, 2084, 2989, 3265, 3488
MbiI	CCGCTC	1 cut(s)	1517
MflI	RGATCY	4 cut(s)	469, 853, 1477, 1870
MhlI	GDGCHC	3 cut(s)	267, 782, 1904
MlsI	TGGCCA	2 cut(s)	1930, 1960
MluNI	TGGCCA	2 cut(s)	1930, 1960
MlyI	GAGTC	5 cut(s)	128, 625, 945, 2081, 2239
MmeI	TCCRAC	5 cut(s)	111, 168, 937, 1216, 3348
Mox20I	TGGCCA	2 cut(s)	1930, 1960
Mph1103I	ATGCAT	2 cut(s)	1742, 2352
MroXI	GAANNNNTTC	1 cut(s)	483
MscI	TGGCCA	2 cut(s)	1930, 1960
MslI	CAYNNNNRTG	3 cut(s)	277, 1121, 2211
Msp20I	TGGCCA	2 cut(s)	1930, 1960
MspA1I	CMGCKG	4 cut(s)	878, 1200, 1264, 1821
MspCI	CTTAAG	2 cut(s)	1499, 1874
MspI	CCGG	5 cut(s)	31, 63, 114, 2475, 3838
MspR9I	CCNGG	7 cut(s)	101, 429, 1861, 1906, 2482, 2807, 3838
Mva1269I	GAATGC	1 cut(s)	1742
MvaI	CCWGG	6 cut(s)	101, 429, 1861, 1906, 2482, 2807
MvnI	CGCG	2 cut(s)	110, 2114
MwoI	GCNNNNNNNGC	5 cut(s)	114, 191, 359, 2780, 3563
NciI	CCSGG	1 cut(s)	3838
NdeI	CATATG	3 cut(s)	1750, 2759, 3952
NheI	GCTAGC	1 cut(s)	233
NlaIV	GGNNCC	9 cut(s)	94, 554, 925, 1297, 1676, 2340, 2479, 3254, 3927
NmeAIII	GCCGAG	3 cut(s)	209, 3281, 3332
NmuCI	GTSAC	6 cut(s)	329, 716, 871, 2073, 2242, 3283
NsbI	TGCGCA	1 cut(s)	2775
NsiI	ATGCAT	2 cut(s)	1742, 2352
NspV	TTCGAA	1 cut(s)	2927
OliI	CACNNNNGTG	1 cut(s)	1121
PaeI	GCATGC	2 cut(s)	2013, 3038
PagI	TCATGA	2 cut(s)	301, 433
PciI	ACATGT	4 cut(s)	272, 691, 1635, 3319
PciSI	GCTCTTC	2 cut(s)	1892, 3616
PcsI	WCGNNNNNNNCGW	1 cut(s)	1607
PctI	GAATGC	1 cut(s)	1742
PdmI	GAANNNNTTC	1 cut(s)	483
PfeI	GAWTC	6 cut(s)	812, 973, 2657, 2924, 2942, 3474
PflMI	CCANNNNNTGG	1 cut(s)	398
PinAI	ACCGGT	1 cut(s)	30
Ple19I	CGATCG	1 cut(s)	1852
PleI	GAGTC	5 cut(s)	127, 625, 945, 2080, 2239
PmaCI	CACGTG	1 cut(s)	1119
PmlI	CACGTG	1 cut(s)	1119
PpsI	GAGTC	5 cut(s)	127, 625, 945, 2080, 2239
Ppu21I	YACGTR	3 cut(s)	1119, 2990, 3489
PpuMI	RGGWCCY	1 cut(s)	598
PscI	ACATGT	4 cut(s)	272, 691, 1635, 3319
PshAI	GACNNNNGTC	2 cut(s)	1636, 3264
PshBI	ATTAAT	1 cut(s)	2030
Psp124BI	GAGCTC	1 cut(s)	267
Psp5II	RGGWCCY	1 cut(s)	598
Psp6I	CCWGG	6 cut(s)	99, 427, 1859, 1904, 2480, 2805
PspCI	CACGTG	1 cut(s)	1119
PspEI	GGTNACC	1 cut(s)	716
PspGI	CCWGG	6 cut(s)	99, 427, 1859, 1904, 2480, 2805
PspN4I	GGNNCC	9 cut(s)	94, 554, 925, 1297, 1676, 2340, 2479, 3254, 3927
PspPI	GGNCC	7 cut(s)	159, 425, 598, 1675, 1863, 2803, 3944
PspPPI	RGGWCCY	1 cut(s)	598
PstI	CTGCAG	2 cut(s)	835, 3405
PstNI	CAGNNNCTG	3 cut(s)	1406, 1697, 3409
PsuI	RGATCY	4 cut(s)	469, 853, 1477, 1870
PvuI	CGATCG	1 cut(s)	1852
PvuII	CAGCTG	2 cut(s)	878, 1200
RgaI	GCGATCGC	1 cut(s)	1852
RseI	CAYNNNNRTG	3 cut(s)	277, 1121, 2211
SacI	GAGCTC	1 cut(s)	267
SapI	GCTCTTC	2 cut(s)	1892, 3616
Sau96I	GGNCC	7 cut(s)	159, 425, 598, 1675, 1863, 2803, 3944
ScaI	AGTACT	1 cut(s)	3758
SchI	GAGTC	5 cut(s)	128, 625, 945, 2081, 2239
ScrFI	CCNGG	7 cut(s)	101, 429, 1861, 1906, 2482, 2807, 3838
SduI	GDGCHC	3 cut(s)	267, 782, 1904
SfaAI	GCGATCGC	1 cut(s)	1852
SfcI	CTRYAG	4 cut(s)	245, 565, 831, 3401
SfuI	TTCGAA	1 cut(s)	2927
SgfI	GCGATCGC	1 cut(s)	1852
SinI	GGWCC	6 cut(s)	159, 425, 598, 1675, 1863, 3944
SmiMI	CAYNNNNRTG	3 cut(s)	277, 1121, 2211
SmlI	CTYRAG	5 cut(s)	1499, 1874, 2222, 2816, 3608
SmoI	CTYRAG	5 cut(s)	1499, 1874, 2222, 2816, 3608
SnaBI	TACGTA	1 cut(s)	3489
SphI	GCATGC	2 cut(s)	2013, 3038
SsiI	CCGC	9 cut(s)	1264, 1517, 1821, 2069, 2114, 2236, 3108, 3418, 3848
SspI	AATATT	2 cut(s)	546, 3739
SspMI	CTAG	4 cut(s)	234, 857, 992, 3617
SstI	GAGCTC	1 cut(s)	267
StyD4I	CCNGG	7 cut(s)	99, 427, 1859, 1904, 2480, 2805, 3836
TaiI	ACGT	9 cut(s)	143, 754, 810, 1121, 1941, 2087, 2992, 3268, 3491
TatI	WGTACW	4 cut(s)	1372, 1495, 2682, 3756
TfiI	GAWTC	6 cut(s)	812, 973, 2657, 2924, 2942, 3474
TscAI	CASTG	8 cut(s)	361, 1231, 1459, 1861, 2926, 3410, 3462, 3861
TseFI	GTSAC	6 cut(s)	329, 716, 871, 2073, 2242, 3283
Tsp45I	GTSAC	6 cut(s)	329, 716, 871, 2073, 2242, 3283
TspGWI	ACGGA	5 cut(s)	194, 502, 605, 1109, 2464
TspRI	CASTG	8 cut(s)	361, 1231, 1459, 1861, 2926, 3410, 3462, 3861
Van91I	CCANNNNNTGG	1 cut(s)	398
Vha464I	CTTAAG	2 cut(s)	1499, 1874
VpaK11BI	GGWCC	6 cut(s)	159, 425, 598, 1675, 1863, 3944
VspI	ATTAAT	1 cut(s)	2030
XapI	RAATTY	7 cut(s)	742, 932, 1129, 2464, 2855, 2892, 3439
XmnI	GAANNNNTTC	1 cut(s)	483
XspI	CTAG	4 cut(s)	234, 857, 992, 3617
ZrmI	AGTACT	1 cut(s)	3758
Zsp2I	ATGCAT	2 cut(s)	1742, 2352

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.