RLG00000012731

Egg cell-secreted protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0001106
Physical Location & Seq
Reverse (-)
21703610 .. 21704557
948 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012731

Sequence Viewer

Length: 444 bp
ATGGCTTACACATCTAAGCTTTTCCTTCTCACAGCTCTGCTTGTTCTGACCATGTCATTTGTGGTCAGTACTTCTCGATCATCGAATTACCCAACTGGGAGAAAATCAAACCTTGCAGCCAGATTGAACTTGGATGAAGAATCATCAACCTGCTGGGAGTCTTTATTTCAGCTCCAGGCATGCAGTAAAGAGGTTGTCTTGTTCTTCCTCAATGGTGAGACTTACTTAGGAGACGGCTGTTGCCAAGCCATTAGAACCATTGAGCGCCAGTGTTGGCCAGCCTTGCTCGGGTCCCTAGGGCTTACCATTGAAGAGACTGATATACTAAAGGGTTACTGCGATGAAGCTGATCAAATCCAATCACCTCCGACATCGCCTTCACCACCATCTGTTCATCAGCCTACTGGAAAACTTGTTCCGCATCTGGACAAGTTGATTCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

148

Amino Acids

16.16

Weight (kDa)

5.08

Isoelectric Point (pI)

58.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 50 - 114 1.5e-18 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 158
AciI CCGC 1 cut(s) 419
AcoI YGGCCR 1 cut(s) 275
AfaI GTAC 1 cut(s) 70
AfiI CCNNNNNNNGG 1 cut(s) 288
AgsI TTSAA 2 cut(s) 127, 311
AjnI CCWGG 1 cut(s) 174
AloI GAACNNNNNNTCC 2 cut(s) 399, 431
AluBI AGCT 4 cut(s) 19, 35, 172, 347
AluI AGCT 4 cut(s) 19, 35, 172, 347
Alw26I GTCTC 3 cut(s) 212, 225, 308
Ama87I CYCGRG 1 cut(s) 287
AoxI GGCC 1 cut(s) 275
ApeKI GCWGC 1 cut(s) 116
AspA2I CCTAGG 1 cut(s) 295
AspLEI GCGC 1 cut(s) 267
AspS9I GGNCC 1 cut(s) 291
AsuHPI GGTGA 3 cut(s) 227, 354, 372
AvaI CYCGRG 1 cut(s) 287
AvaII GGWCC 1 cut(s) 291
AvrII CCTAGG 1 cut(s) 295
BalI TGGCCA 1 cut(s) 277
BbvI GCAGC 1 cut(s) 128
BccI CCATC 1 cut(s) 394
BceAI ACGGC 1 cut(s) 250
BciT130I CCWGG 1 cut(s) 176
BclI TGATCA 1 cut(s) 349
BcoDI GTCTC 3 cut(s) 212, 225, 308
BfaI CTAG 1 cut(s) 296
BfoI RGCGCY 1 cut(s) 268
BfuAI ACCTGC 1 cut(s) 158
BisI GCNGC 1 cut(s) 117
BlnI CCTAGG 1 cut(s) 295
BlsI GCNGC 1 cut(s) 118
BmcAI AGTACT 1 cut(s) 70
Bme1390I CCNGG 1 cut(s) 176
Bme18I GGWCC 1 cut(s) 291
BmeT110I CYCGRG 1 cut(s) 287
BmgT120I GGNCC 1 cut(s) 291
BmiI GGNNCC 2 cut(s) 292, 293
BmrFI CCNGG 1 cut(s) 176
BmrI ACTGGG 1 cut(s) 105
BmsI GCATC 1 cut(s) 430
BmuI ACTGGG 1 cut(s) 105
BpmI CTGGAG 1 cut(s) 158
BsaJI CCNNGG 1 cut(s) 295
Bsc4I CCNNNNNNNGG 1 cut(s) 288
Bse1I ACTGG 3 cut(s) 100, 268, 409
BseBI CCWGG 1 cut(s) 176
BseDI CCNNGG 1 cut(s) 295
BseGI GGATG 1 cut(s) 139
BseLI CCNNNNNNNGG 1 cut(s) 288
BseNI ACTGG 3 cut(s) 100, 268, 409
BseXI GCAGC 1 cut(s) 128
BseYI CCCAGC 1 cut(s) 153
BshFI GGCC 1 cut(s) 277
BsiHKCI CYCGRG 1 cut(s) 287
BslFI GGGAC 1 cut(s) 277
BslI CCNNNNNNNGG 1 cut(s) 288
BsmAI GTCTC 3 cut(s) 212, 225, 308
BsmBI CGTCTC 1 cut(s) 225
BsmFI GGGAC 1 cut(s) 277
BsnI GGCC 1 cut(s) 277
BsoBI CYCGRG 1 cut(s) 287
Bsp143I GATC 2 cut(s) 77, 349
BspACI CCGC 1 cut(s) 419
BspANI GGCC 1 cut(s) 277
BspLI GGNNCC 2 cut(s) 292, 293
BspMI ACCTGC 1 cut(s) 158
BsrI ACTGG 3 cut(s) 100, 268, 409
BssECI CCNNGG 1 cut(s) 295
BssMI GATC 2 cut(s) 77, 349
BssT1I CCWWGG 1 cut(s) 295
Bst2UI CCWGG 1 cut(s) 176
Bst6I CTCTTC 1 cut(s) 306
BstC8I GCNNGC 2 cut(s) 181, 279
BstDEI CTNAG 2 cut(s) 15, 226
BstF5I GGATG 1 cut(s) 139
BstH2I RGCGCY 1 cut(s) 268
BstHHI GCGC 1 cut(s) 267
BstKTI GATC 2 cut(s) 80, 352
BstMAI GTCTC 3 cut(s) 212, 225, 308
BstMBI GATC 2 cut(s) 77, 349
BstMWI GCNNNNNNNGC 1 cut(s) 283
BstNI CCWGG 1 cut(s) 176
BstNSI RCATGY 1 cut(s) 183
BstSCI CCNGG 1 cut(s) 174
BstV1I GCAGC 1 cut(s) 128
BsuRI GGCC 1 cut(s) 277
BtgZI GCGATG 2 cut(s) 354, 357
BtsCI GGATG 1 cut(s) 139
BtsIMutI CAGTG 1 cut(s) 275
BveI ACCTGC 1 cut(s) 158
Cac8I GCNNGC 2 cut(s) 181, 279
CfoI GCGC 1 cut(s) 267
Cfr13I GGNCC 1 cut(s) 291
Csp6I GTAC 1 cut(s) 69
CviAII CATG 2 cut(s) 52, 180
CviQI GTAC 1 cut(s) 69
DdeI CTNAG 2 cut(s) 15, 226
DpnI GATC 2 cut(s) 79, 351
DpnII GATC 2 cut(s) 77, 349
EaeI YGGCCR 1 cut(s) 275
Eam1104I CTCTTC 1 cut(s) 306
EarI CTCTTC 1 cut(s) 306
Eco130I CCWWGG 1 cut(s) 295
Eco47I GGWCC 1 cut(s) 291
Eco88I CYCGRG 1 cut(s) 287
EcoO109I RGGNCCY 1 cut(s) 291
EcoRII CCWGG 1 cut(s) 174
EcoT14I CCWWGG 1 cut(s) 295
ErhI CCWWGG 1 cut(s) 295
Esp3I CGTCTC 1 cut(s) 225
FaeI CATG 2 cut(s) 55, 183
FaiI YATR 3 cut(s) 53, 181, 323
FaqI GGGAC 1 cut(s) 277
FatI CATG 2 cut(s) 51, 179
FbaI TGATCA 1 cut(s) 349
Fnu4HI GCNGC 1 cut(s) 117
FokI GGATG 1 cut(s) 146
Fsp4HI GCNGC 1 cut(s) 117
FspBI CTAG 1 cut(s) 296
GlaI GCGC 1 cut(s) 266
GluI GCNGC 1 cut(s) 117
GsaI CCCAGC 1 cut(s) 157
GsuI CTGGAG 1 cut(s) 158
HaeII RGCGCY 1 cut(s) 268
HaeIII GGCC 1 cut(s) 277
HhaI GCGC 1 cut(s) 267
Hin1II CATG 2 cut(s) 55, 183
Hin6I GCGC 1 cut(s) 265
HinP1I GCGC 1 cut(s) 265
HindIII AAGCTT 1 cut(s) 17
HinfI GANTC 3 cut(s) 140, 158, 436
HphI GGTGA 3 cut(s) 227, 354, 372
Hpy188I TCNGA 2 cut(s) 48, 369
Hpy188III TCNNGA 2 cut(s) 75, 425
HpyAV CCTTC 2 cut(s) 35, 387
HpyCH4V TGCA 2 cut(s) 116, 183
HpyF10VI GCNNNNNNNGC 1 cut(s) 283
HpyF3I CTNAG 2 cut(s) 15, 226
Hsp92II CATG 2 cut(s) 55, 183
HspAI GCGC 1 cut(s) 265
KflI GGGWCCC 1 cut(s) 291
Ksp22I TGATCA 1 cut(s) 349
Kzo9I GATC 2 cut(s) 77, 349
LmnI GCTCC 1 cut(s) 177
Lsp1109I GCAGC 1 cut(s) 128
LweI GCATC 1 cut(s) 430
MaeI CTAG 1 cut(s) 296
MaeIII GTNAC 1 cut(s) 332
MalI GATC 2 cut(s) 79, 351
MboI GATC 2 cut(s) 77, 349
MboII GAAGA 3 cut(s) 149, 196, 323
MlsI TGGCCA 1 cut(s) 277
MluCI AATT 1 cut(s) 85
MluNI TGGCCA 1 cut(s) 277
MlyI GAGTC 1 cut(s) 167
MmeI TCCRAC 1 cut(s) 392
MnlI CCTC 3 cut(s) 184, 218, 375
Mox20I TGGCCA 1 cut(s) 277
MscI TGGCCA 1 cut(s) 277
Msp20I TGGCCA 1 cut(s) 277
MspR9I CCNGG 1 cut(s) 176
MvaI CCWGG 1 cut(s) 176
MwoI GCNNNNNNNGC 1 cut(s) 283
NdeII GATC 2 cut(s) 77, 349
NlaIII CATG 2 cut(s) 55, 183
NlaIV GGNNCC 2 cut(s) 292, 293
NspI RCATGY 1 cut(s) 183
PaeI GCATGC 1 cut(s) 183
PfeI GAWTC 2 cut(s) 140, 436
PflFI GACNNNGTC 1 cut(s) 52
PkrI GCNGC 1 cut(s) 118
PleI GAGTC 1 cut(s) 166
PpsI GAGTC 1 cut(s) 166
PpuMI RGGWCCY 1 cut(s) 291
Psp5II RGGWCCY 1 cut(s) 291
Psp6I CCWGG 1 cut(s) 174
PspFI CCCAGC 1 cut(s) 153
PspGI CCWGG 1 cut(s) 174
PspN4I GGNNCC 2 cut(s) 292, 293
PspPI GGNCC 1 cut(s) 291
PspPPI RGGWCCY 1 cut(s) 291
PsyI GACNNNGTC 1 cut(s) 52
RsaI GTAC 1 cut(s) 70
RsaNI GTAC 1 cut(s) 69
SatI GCNGC 1 cut(s) 117
Sau3AI GATC 2 cut(s) 77, 349
Sau96I GGNCC 1 cut(s) 291
ScaI AGTACT 1 cut(s) 70
SchI GAGTC 1 cut(s) 167
ScrFI CCNGG 1 cut(s) 176
SetI ASST 8 cut(s) 21, 37, 114, 152, 174, 195, 349, 367
SfaNI GCATC 1 cut(s) 430
SinI GGWCC 1 cut(s) 291
SphI GCATGC 1 cut(s) 183
Sse9I AATT 1 cut(s) 85
SsiI CCGC 1 cut(s) 419
SspMI CTAG 1 cut(s) 296
StyD4I CCNGG 1 cut(s) 174
StyI CCWWGG 1 cut(s) 295
TaqI TCGA 2 cut(s) 76, 83
TasI AATT 1 cut(s) 85
TatI WGTACW 1 cut(s) 68
TfiI GAWTC 2 cut(s) 140, 436
TscAI CASTG 1 cut(s) 275
TseI GCWGC 1 cut(s) 116
TspDTI ATGAA 3 cut(s) 150, 357, 383
TspGWI ACGGA 1 cut(s) 429
TspRI CASTG 1 cut(s) 275
Tth111I GACNNNGTC 1 cut(s) 52
VpaK11BI GGWCC 1 cut(s) 291
XceI RCATGY 1 cut(s) 183
XcmI CCANNNNNNNNNTGG 2 cut(s) 58, 127
XmaJI CCTAGG 1 cut(s) 295
XspI CTAG 1 cut(s) 296
ZrmI AGTACT 1 cut(s) 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.