Rh6BG205000

Egg cell-secreted protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0001106
Physical Location & Seq
Forward (+)
38565549 .. 38566371
823 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG205000.1

Sequence Viewer

Length: 561 bp
ATGCACGTAACGCTTTCGCATCTCCATCACTACTTAAACCCTCGCTTCTCCTCTGTCCCTTCACACATGACACATTATCTCCTGGATAACAGAATCACAATGGCCTTTAACACTTCCAACCTCTTCCTAACTCTCACTGCTCTCATTGCCCTGACCATGCCGTTCATGGCTTCTGCTTATCGACCCTTGGTGACGAATGACTCAACCGGATCAGATCCAAGCCTGGCAGCTAGGTTGAATTTGGATGGAGAATCCTCCAACTGCTGGGATTCTTTGCTTCAGCTCCAGTCATGTAGCGGTGAGGCTATAATGTTTTTCTTGAATGGCCAGACTTCTTTGGGTGATGGATGTTGTGAAGCTATTCGGGTAATCGAGCACCAGTGCTGGCCTGCCTTGCTCGGCTCGCTAGGGTTTACCACGGAAGAGACTGATATTCTCAAAGGTTATTGCGACGAGGCTGAACAAACTCATCATTCTCCTCCGTCGATACCATCACCATCGTCCCTTTTTCATCCTAATAGAGCAGTTTATGTTCCTTATCAGGCTGATATGGCTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

186

Amino Acids

20.5

Weight (kDa)

5.26

Isoelectric Point (pI)

54.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 87 - 151 3.3e-17 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 264
AciI CCGC 1 cut(s) 297
AclWI GGATC 2 cut(s) 209, 217
AcoI YGGCCR 1 cut(s) 325
AcsI RAATTY 1 cut(s) 238
AcuI CTGAAG 1 cut(s) 263
AfiI CCNNNNNNNGG 1 cut(s) 264
AgsI TTSAA 2 cut(s) 238, 322
AjnI CCWGG 2 cut(s) 81, 222
AluBI AGCT 3 cut(s) 230, 283, 359
AluI AGCT 3 cut(s) 230, 283, 359
Alw21I GWGCWC 1 cut(s) 378
Alw26I GTCTC 1 cut(s) 419
AlwI GGATC 2 cut(s) 209, 217
AoxI GGCC 3 cut(s) 102, 325, 386
ApeKI GCWGC 1 cut(s) 227
ApoI RAATTY 1 cut(s) 238
Asp700I GAANNNNTTC 1 cut(s) 360
AsuHPI GGTGA 4 cut(s) 202, 311, 353, 486
BalI TGGCCA 1 cut(s) 327
Bbv12I GWGCWC 1 cut(s) 378
BbvI GCAGC 1 cut(s) 239
BccI CCATC 5 cut(s) 33, 239, 338, 499, 505
BceAI ACGGC 1 cut(s) 145
BciT130I CCWGG 2 cut(s) 83, 224
BcoDI GTCTC 1 cut(s) 419
BfaI CTAG 2 cut(s) 231, 407
BisI GCNGC 1 cut(s) 228
BlsI GCNGC 1 cut(s) 229
Bme1390I CCNGG 2 cut(s) 83, 224
BmiI GGNNCC 1 cut(s) 555
BmrFI CCNGG 2 cut(s) 83, 224
BmsI GCATC 1 cut(s) 28
BpmI CTGGAG 1 cut(s) 269
BsaAI YACGTR 1 cut(s) 7
BsaJI CCNNGG 2 cut(s) 186, 417
BsaWI WCCGGW 1 cut(s) 206
BsaXI ACNNNNNCTCC 2 cut(s) 63, 93
Bsc4I CCNNNNNNNGG 1 cut(s) 264
Bse1I ACTGG 2 cut(s) 286, 379
Bse3DI GCAATG 1 cut(s) 144
BseBI CCWGG 2 cut(s) 83, 224
BseDI CCNNGG 2 cut(s) 186, 417
BseGI GGATG 3 cut(s) 250, 353, 511
BseLI CCNNNNNNNGG 1 cut(s) 264
BseMI GCAATG 1 cut(s) 144
BseNI ACTGG 2 cut(s) 286, 379
BseRI GAGGAG 2 cut(s) 40, 468
BseXI GCAGC 1 cut(s) 239
BseYI CCCAGC 1 cut(s) 264
BshFI GGCC 3 cut(s) 104, 327, 388
BsiHKAI GWGCWC 1 cut(s) 378
BsiSI CCGG 1 cut(s) 207
BslFI GGGAC 2 cut(s) 41, 487
BslI CCNNNNNNNGG 1 cut(s) 264
BsmAI GTCTC 1 cut(s) 419
BsmFI GGGAC 2 cut(s) 41, 487
BsnI GGCC 3 cut(s) 104, 327, 388
Bsp1286I GDGCHC 1 cut(s) 378
Bsp143I GATC 2 cut(s) 209, 214
BspACI CCGC 1 cut(s) 297
BspANI GGCC 3 cut(s) 104, 327, 388
BspLI GGNNCC 1 cut(s) 555
BspPI GGATC 2 cut(s) 209, 217
BsrDI GCAATG 1 cut(s) 144
BsrI ACTGG 2 cut(s) 286, 379
BssECI CCNNGG 2 cut(s) 186, 417
BssMI GATC 2 cut(s) 209, 214
BssT1I CCWWGG 1 cut(s) 186
Bst2UI CCWGG 2 cut(s) 83, 224
Bst6I CTCTTC 2 cut(s) 128, 417
BstBAI YACGTR 1 cut(s) 7
BstC8I GCNNGC 3 cut(s) 386, 390, 404
BstDSI CCRYGG 1 cut(s) 417
BstF5I GGATG 3 cut(s) 250, 353, 511
BstKTI GATC 2 cut(s) 212, 217
BstMAI GTCTC 1 cut(s) 419
BstMBI GATC 2 cut(s) 209, 214
BstMWI GCNNNNNNNGC 5 cut(s) 10, 146, 394, 403, 551
BstNI CCWGG 2 cut(s) 83, 224
BstSCI CCNGG 2 cut(s) 81, 222
BstV1I GCAGC 1 cut(s) 239
BstX2I RGATCY 1 cut(s) 214
BstYI RGATCY 1 cut(s) 214
BsuRI GGCC 3 cut(s) 104, 327, 388
BtgI CCRYGG 1 cut(s) 417
BtsCI GGATG 3 cut(s) 250, 353, 511
BtsI GCAGTG 1 cut(s) 135
BtsIMutI CAGTG 2 cut(s) 135, 386
Cac8I GCNNGC 3 cut(s) 386, 390, 404
CviAII CATG 4 cut(s) 67, 157, 166, 291
DpnI GATC 2 cut(s) 211, 216
DpnII GATC 2 cut(s) 209, 214
EaeI YGGCCR 1 cut(s) 325
Eam1104I CTCTTC 2 cut(s) 128, 417
EarI CTCTTC 2 cut(s) 128, 417
Eco130I CCWWGG 1 cut(s) 186
Eco57I CTGAAG 1 cut(s) 263
EcoRII CCWGG 2 cut(s) 81, 222
EcoT14I CCWWGG 1 cut(s) 186
ErhI CCWWGG 1 cut(s) 186
FaeI CATG 4 cut(s) 70, 160, 169, 294
FaiI YATR 7 cut(s) 68, 158, 167, 292, 308, 531, 551
FaqI GGGAC 2 cut(s) 41, 487
FatI CATG 4 cut(s) 66, 156, 165, 290
Fnu4HI GCNGC 1 cut(s) 228
FokI GGATG 3 cut(s) 257, 360, 498
Fsp4HI GCNGC 1 cut(s) 228
FspBI CTAG 2 cut(s) 231, 407
GluI GCNGC 1 cut(s) 228
GsaI CCCAGC 1 cut(s) 268
GsuI CTGGAG 1 cut(s) 269
HaeIII GGCC 3 cut(s) 104, 327, 388
HapII CCGG 1 cut(s) 207
Hin1II CATG 4 cut(s) 70, 160, 169, 294
HinfI GANTC 4 cut(s) 93, 200, 251, 269
HpaII CCGG 1 cut(s) 207
HphI GGTGA 4 cut(s) 202, 311, 353, 486
Hpy166II GTNNAC 1 cut(s) 414
Hpy188I TCNGA 1 cut(s) 214
Hpy188III TCNNGA 1 cut(s) 319
Hpy8I GTNNAC 1 cut(s) 414
Hpy99I CGWCG 2 cut(s) 455, 487
HpyAV CCTTC 1 cut(s) 69
HpyCH4IV ACGT 1 cut(s) 6
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 5 cut(s) 10, 146, 394, 403, 551
HpySE526I ACGT 1 cut(s) 6
Hsp92II CATG 4 cut(s) 70, 160, 169, 294
Kzo9I GATC 2 cut(s) 209, 214
LmnI GCTCC 2 cut(s) 288, 559
Lsp1109I GCAGC 1 cut(s) 239
LweI GCATC 1 cut(s) 28
MaeI CTAG 2 cut(s) 231, 407
MaeII ACGT 1 cut(s) 6
MaeIII GTNAC 2 cut(s) 7, 190
MalI GATC 2 cut(s) 211, 216
MboI GATC 2 cut(s) 209, 214
MboII GAAGA 2 cut(s) 115, 434
MflI RGATCY 1 cut(s) 214
MhlI GDGCHC 1 cut(s) 378
MlsI TGGCCA 1 cut(s) 327
MluCI AATT 1 cut(s) 238
MluNI TGGCCA 1 cut(s) 327
MlyI GAGTC 1 cut(s) 194
MmeI TCCRAC 2 cut(s) 141, 282
MnlI CCTC 7 cut(s) 51, 61, 131, 265, 295, 448, 489
Mox20I TGGCCA 1 cut(s) 327
MroXI GAANNNNTTC 1 cut(s) 360
MscI TGGCCA 1 cut(s) 327
MseI TTAA 2 cut(s) 35, 108
Msp20I TGGCCA 1 cut(s) 327
MspI CCGG 1 cut(s) 207
MspR9I CCNGG 2 cut(s) 83, 224
MvaI CCWGG 2 cut(s) 83, 224
MwoI GCNNNNNNNGC 5 cut(s) 10, 146, 394, 403, 551
NdeII GATC 2 cut(s) 209, 214
NlaIII CATG 4 cut(s) 70, 160, 169, 294
NlaIV GGNNCC 1 cut(s) 555
NmeAIII GCCGAG 1 cut(s) 378
NmuCI GTSAC 1 cut(s) 190
PdmI GAANNNNTTC 1 cut(s) 360
PfeI GAWTC 3 cut(s) 93, 251, 269
PflMI CCANNNNNTGG 1 cut(s) 264
PfoI TCCNGGA 1 cut(s) 81
PkrI GCNGC 1 cut(s) 229
PleI GAGTC 1 cut(s) 194
PpsI GAGTC 1 cut(s) 194
Ppu21I YACGTR 1 cut(s) 7
Psp6I CCWGG 2 cut(s) 81, 222
PspFI CCCAGC 1 cut(s) 264
PspGI CCWGG 2 cut(s) 81, 222
PspN4I GGNNCC 1 cut(s) 555
PsuI RGATCY 1 cut(s) 214
SaqAI TTAA 2 cut(s) 35, 108
SatI GCNGC 1 cut(s) 228
Sau3AI GATC 2 cut(s) 209, 214
SchI GAGTC 1 cut(s) 194
ScrFI CCNGG 2 cut(s) 83, 224
SduI GDGCHC 1 cut(s) 378
SetI ASST 7 cut(s) 9, 123, 232, 236, 285, 361, 445
SfaNI GCATC 1 cut(s) 28
Sse9I AATT 1 cut(s) 238
SsiI CCGC 1 cut(s) 297
SspMI CTAG 2 cut(s) 231, 407
StyD4I CCNGG 2 cut(s) 81, 222
StyI CCWWGG 1 cut(s) 186
TaiI ACGT 1 cut(s) 9
TaqI TCGA 3 cut(s) 181, 372, 485
TasI AATT 1 cut(s) 238
TfiI GAWTC 3 cut(s) 93, 251, 269
Tru1I TTAA 2 cut(s) 35, 108
Tru9I TTAA 2 cut(s) 35, 108
TscAI CASTG 2 cut(s) 142, 386
TseFI GTSAC 1 cut(s) 190
TseI GCWGC 1 cut(s) 227
Tsp45I GTSAC 1 cut(s) 190
TspDTI ATGAA 2 cut(s) 154, 500
TspGWI ACGGA 2 cut(s) 434, 471
TspRI CASTG 2 cut(s) 142, 386
Van91I CCANNNNNTGG 1 cut(s) 264
XapI RAATTY 1 cut(s) 238
XcmI CCANNNNNNNNNTGG 1 cut(s) 163
XmnI GAANNNNTTC 1 cut(s) 360
XspI CTAG 2 cut(s) 231, 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.