Rh7AG166200

Egg cell-secreted protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0001106
Physical Location & Seq
Reverse (-)
14211100 .. 14211504
405 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG166200.1

Sequence Viewer

Length: 405 bp
ATGGCTTCCAGTTCCAAGCTCTTCCTTCTCATAGCTCTTTTGGCCATGACCATGCCATTCATGGCGAGTTTTGGTCCTTTAATTAATAACCTGCGAATTGGGTCGTACCCCCGGAAACTTGCAACCAGGTTGAATCTGGTTGGAGAATCATCAAACTGCTGGGACTCTCTGCTTCAGCTCCATGCATGTAGTGGCGAAGTTATCTTGTTTTTCTTCAATGGTGAGACTTACTTGGGTCATAGCTGTTGTGAAGCCATTTGGACGATTGAGCATCAGTGTTGGCCTGCCTTGCTCGGCACGCTAGGGTTTACCACTCAAGAGACGGATGTACTCAAAGGTTATTGCGATAAAGCCGATCAAATTCATTCTCCTCCGTCTCCACCATCCCCAGCCACCCTCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

134

Amino Acids

14.69

Weight (kDa)

5.7

Isoelectric Point (pI)

52.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 52 - 116 4.1e-18 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 99
AcoI YGGCCR 1 cut(s) 42
AcsI RAATTY 1 cut(s) 360
AcuI CTGAAG 1 cut(s) 158
AfaI GTAC 2 cut(s) 107, 330
AgsI TTSAA 2 cut(s) 133, 217
AjnI CCWGG 1 cut(s) 125
AluBI AGCT 4 cut(s) 19, 35, 178, 243
AluI AGCT 4 cut(s) 19, 35, 178, 243
Alw26I GTCTC 3 cut(s) 218, 314, 381
AoxI GGCC 2 cut(s) 42, 281
ApoI RAATTY 1 cut(s) 360
AseI ATTAAT 1 cut(s) 84
AspS9I GGNCC 1 cut(s) 74
AsuC2I CCSGG 1 cut(s) 112
AsuHPI GGTGA 1 cut(s) 233
AvaII GGWCC 1 cut(s) 74
BalI TGGCCA 1 cut(s) 44
BccI CCATC 1 cut(s) 391
BciT130I CCWGG 1 cut(s) 127
BcnI CCSGG 1 cut(s) 112
BcoDI GTCTC 3 cut(s) 218, 314, 381
BfaI CTAG 1 cut(s) 302
BfuAI ACCTGC 1 cut(s) 99
Bme1390I CCNGG 2 cut(s) 112, 127
Bme18I GGWCC 1 cut(s) 74
BmgT120I GGNCC 1 cut(s) 74
BmrFI CCNGG 2 cut(s) 112, 127
BmsI GCATC 1 cut(s) 280
BpuEI CTTGAG 1 cut(s) 300
BpuMI CCSGG 1 cut(s) 112
BsaJI CCNNGG 1 cut(s) 110
Bse1I ACTGG 1 cut(s) 9
BseBI CCWGG 1 cut(s) 127
BseDI CCNNGG 1 cut(s) 110
BseGI GGATG 2 cut(s) 331, 383
BseNI ACTGG 1 cut(s) 9
BseRI GAGGAG 1 cut(s) 360
BseYI CCCAGC 2 cut(s) 159, 388
BshFI GGCC 2 cut(s) 44, 283
BsiSI CCGG 1 cut(s) 112
BslFI GGGAC 1 cut(s) 176
BsmAI GTCTC 3 cut(s) 218, 314, 381
BsmBI CGTCTC 2 cut(s) 314, 381
BsmFI GGGAC 1 cut(s) 176
BsnI GGCC 2 cut(s) 44, 283
Bsp143I GATC 1 cut(s) 355
BspANI GGCC 2 cut(s) 44, 283
BspMI ACCTGC 1 cut(s) 99
BspQI GCTCTTC 1 cut(s) 26
BsrI ACTGG 1 cut(s) 9
BssECI CCNNGG 1 cut(s) 110
BssMI GATC 1 cut(s) 355
Bst2UI CCWGG 1 cut(s) 127
Bst6I CTCTTC 1 cut(s) 26
BstC8I GCNNGC 2 cut(s) 285, 299
BstF5I GGATG 2 cut(s) 331, 383
BstKTI GATC 1 cut(s) 358
BstMAI GTCTC 3 cut(s) 218, 314, 381
BstMBI GATC 1 cut(s) 355
BstMWI GCNNNNNNNGC 3 cut(s) 41, 289, 298
BstNI CCWGG 1 cut(s) 127
BstNSI RCATGY 1 cut(s) 189
BstSCI CCNGG 2 cut(s) 110, 125
BsuRI GGCC 2 cut(s) 44, 283
BtsCI GGATG 2 cut(s) 331, 383
BtsIMutI CAGTG 1 cut(s) 281
BveI ACCTGC 1 cut(s) 99
Cac8I GCNNGC 2 cut(s) 285, 299
Cfr13I GGNCC 1 cut(s) 74
CsiI ACCWGGT 1 cut(s) 125
Csp6I GTAC 2 cut(s) 106, 329
CviAII CATG 5 cut(s) 46, 52, 61, 182, 186
CviQI GTAC 2 cut(s) 106, 329
DpnI GATC 1 cut(s) 357
DpnII GATC 1 cut(s) 355
EaeI YGGCCR 1 cut(s) 42
Eam1104I CTCTTC 1 cut(s) 26
EarI CTCTTC 1 cut(s) 26
Eco47I GGWCC 1 cut(s) 74
Eco57I CTGAAG 1 cut(s) 158
EcoRII CCWGG 1 cut(s) 125
EcoT22I ATGCAT 1 cut(s) 187
Esp3I CGTCTC 2 cut(s) 314, 381
FaeI CATG 5 cut(s) 49, 55, 64, 185, 189
FaiI YATR 7 cut(s) 32, 47, 53, 62, 183, 187, 240
FaqI GGGAC 1 cut(s) 176
FatI CATG 5 cut(s) 45, 51, 60, 181, 185
FokI GGATG 2 cut(s) 338, 370
FspBI CTAG 1 cut(s) 302
GsaI CCCAGC 2 cut(s) 163, 392
HaeIII GGCC 2 cut(s) 44, 283
HapII CCGG 1 cut(s) 112
Hin1II CATG 5 cut(s) 49, 55, 64, 185, 189
HinfI GANTC 3 cut(s) 133, 146, 164
HpaII CCGG 1 cut(s) 112
HphI GGTGA 1 cut(s) 233
Hpy166II GTNNAC 1 cut(s) 309
Hpy188III TCNNGA 1 cut(s) 317
Hpy8I GTNNAC 1 cut(s) 309
HpyAV CCTTC 1 cut(s) 35
HpyCH4V TGCA 2 cut(s) 122, 185
HpyF10VI GCNNNNNNNGC 3 cut(s) 41, 289, 298
Hsp92II CATG 5 cut(s) 49, 55, 64, 185, 189
Kzo9I GATC 1 cut(s) 355
LguI GCTCTTC 1 cut(s) 26
LmnI GCTCC 1 cut(s) 183
LpnPI CCDG 8 cut(s) 22, 104, 112, 122, 125, 139, 145, 297
LweI GCATC 1 cut(s) 280
MabI ACCWGGT 1 cut(s) 125
MaeI CTAG 1 cut(s) 302
MalI GATC 1 cut(s) 357
MboI GATC 1 cut(s) 355
MboII GAAGA 2 cut(s) 13, 205
MlsI TGGCCA 1 cut(s) 44
MluCI AATT 3 cut(s) 81, 96, 360
MluNI TGGCCA 1 cut(s) 44
MlyI GAGTC 1 cut(s) 158
MmeI TCCRAC 1 cut(s) 121
MnlI CCTC 1 cut(s) 381
Mox20I TGGCCA 1 cut(s) 44
Mph1103I ATGCAT 1 cut(s) 187
MscI TGGCCA 1 cut(s) 44
MseI TTAA 2 cut(s) 80, 84
MslI CAYNNNNRTG 1 cut(s) 50
Msp20I TGGCCA 1 cut(s) 44
MspI CCGG 1 cut(s) 112
MspR9I CCNGG 2 cut(s) 112, 127
MvaI CCWGG 1 cut(s) 127
MwoI GCNNNNNNNGC 3 cut(s) 41, 289, 298
NciI CCSGG 1 cut(s) 112
NdeII GATC 1 cut(s) 355
NlaIII CATG 5 cut(s) 49, 55, 64, 185, 189
NmeAIII GCCGAG 1 cut(s) 273
NsiI ATGCAT 1 cut(s) 187
NspI RCATGY 1 cut(s) 189
PacI TTAATTAA 1 cut(s) 84
PciSI GCTCTTC 1 cut(s) 26
PfeI GAWTC 2 cut(s) 133, 146
PleI GAGTC 1 cut(s) 158
PpsI GAGTC 1 cut(s) 158
PshBI ATTAAT 1 cut(s) 84
Psp6I CCWGG 1 cut(s) 125
PspFI CCCAGC 2 cut(s) 159, 388
PspGI CCWGG 1 cut(s) 125
PspPI GGNCC 1 cut(s) 74
RsaI GTAC 2 cut(s) 107, 330
RsaNI GTAC 2 cut(s) 106, 329
RseI CAYNNNNRTG 1 cut(s) 50
SapI GCTCTTC 1 cut(s) 26
SaqAI TTAA 2 cut(s) 80, 84
Sau3AI GATC 1 cut(s) 355
Sau96I GGNCC 1 cut(s) 74
SchI GAGTC 1 cut(s) 158
ScrFI CCNGG 2 cut(s) 112, 127
SetI ASST 7 cut(s) 21, 37, 93, 131, 180, 245, 340
SexAI ACCWGGT 1 cut(s) 125
SfaNI GCATC 1 cut(s) 280
SinI GGWCC 1 cut(s) 74
SmiMI CAYNNNNRTG 1 cut(s) 50
SmlI CTYRAG 1 cut(s) 315
SmoI CTYRAG 1 cut(s) 315
Sse9I AATT 3 cut(s) 81, 96, 360
SspMI CTAG 1 cut(s) 302
StyD4I CCNGG 2 cut(s) 110, 125
TasI AATT 3 cut(s) 81, 96, 360
TatI WGTACW 1 cut(s) 328
TfiI GAWTC 2 cut(s) 133, 146
Tru1I TTAA 2 cut(s) 80, 84
Tru9I TTAA 2 cut(s) 80, 84
TscAI CASTG 1 cut(s) 281
TspDTI ATGAA 2 cut(s) 49, 353
TspGWI ACGGA 2 cut(s) 338, 363
TspRI CASTG 1 cut(s) 281
VpaK11BI GGWCC 1 cut(s) 74
VspI ATTAAT 1 cut(s) 84
XapI RAATTY 1 cut(s) 360
XceI RCATGY 1 cut(s) 189
XcmI CCANNNNNNNNNTGG 3 cut(s) 58, 133, 188
XspI CTAG 1 cut(s) 302
Zsp2I ATGCAT 1 cut(s) 187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.