Rorug06G0165500

Egg cell-secreted protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001106
Physical Location & Seq
Forward (+)
23770032 .. 23777384
7353 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0165500.1

Sequence Viewer

Length: 2823 bp
ATGATTGCCGGTTTCCATCAATGTAGAATCAGCAAATCTCAATCTCCGCATTCATCATCCTCTTCTTTTCATTCCTTCCTTTTAGGGGGCGGGAAGACTGCAAAGGTGTACTTGAGTTGCATAGAAATGGCCCTCTCAGATATTTATGCATCTCATCCTTACTTCAAGACGAGGATAGTATTGAACACAAGGAACTCCAAACAAACTGTTGTTGGTGCAGCTGCGGCAGCTCTGGATCTGATAAAGAATGCAGAAGTTCTAGCAATATTAGGGCCTGAAACATCAATGCAGGCGAGCTTCATTGTTAATTTGGGAGACGAAGCTCATGTGCCCATTTTATCATTTTCTGCATCAGCACCTTATCTTACTTCGCTCCGGAGCCCTTACTTTTTTAGAATTACTCAAACCGACTCATATCAAGTGAAAGCCATTAGTAGCATTGTTCAAAATTTTGGATGGAGACAAGTTGTACCAATCTACGTGGACAATATGTATGGGGAGGGAATCATACCTTTTCTCATTGATGCCTTGCAAGATGTTGATGCTCATGTCCCTTACCGTAGTGTCATTTCCCCATCAGCAACTGGTGACCAGATTATAAAAGAGCTTTACAAATTAATGACAATGCAAACTAGAGTCTTCATCGTGCATATGAAGTCCAATCTTTGCTTTAAGCTGTTTTCCAAGGCAAAAGAGATAGGAATGATGAGTAAAGGATATGTTTGGATCTTGACCGATGGGATTTCTAATCGTTTAAATTCGATGAATTCTTCAGCCATAGCTTCCATGGAAGGTGTGTTAGGTATCAAAACATTCATCCCGAGAACAACAGAACTTGAAAAATTCGAGCTCCGGTGGAAAGGGCAATTCCAACAAGACAACCCAACCATTATAGATGTTCAATTGGATGTTTATGCACTTCAGGCTTATGATGCTGCTTTTGCATTAGCCGTGTCAATTGAAAATGTTGGGATCAGTACAAGCTCTGAAATCCACAAGGATACTTCCTTCAACTCAACTGACCTTGATACTTATAAGGTCTCTGAAAATGGTCCTAAACTTGCTGAAGCATTATCAATTACTAGATTCAAAGGCATAGCTGGAGACTTCAAGCTTGCAGAAGGGCAACTTCAGTCATCAACTTTTACAATTGTTAATATAAAAGGTGAGGGAGGAAAGCCAGTTGCATTTTGGACTCCGCAAAATCAAATGGTGAAAAAGTTGAATGACTCAACAAACACAAGCATCCTTTCAACTAATTCCAAGAGCAATCTTGGACCAATTAAATGGCCTGGAAATTCTCTCTCTGTTCCAAGGGGATGGGAGATACCAACAAATGGCAAGAAGTTGATAATAGGAGTTCCTGTCAAGATTGATTTTACTGAATTTGTTAAGATCTCGAAAGATCCAAAGACCGGCACAATGGATGTCACTGGGTTCTGTATTGATGTCTTTCATGCTGCATTGAAAGTACTACCTTATGCTCTTCCTTATGAGTTCGCTCCCTTCACAAACCCTGATGGCACTAGTGCTGGCAATTATAATGATTTGGTCTATCAAGTATATCTTGGGAAGTTTGATGCCGTCGTTGGAGATACAACAATTAGAGCAAATAGGTCCTTGTACGTGGACTTTACAATGCCATACACAGAATCTGGTGTTGTAATGGTGGTGCCAATCATAGACACAACGAGCAAGAATGCATGGGTTTTCTTGAAGCCTTTGACTTGGGACCTGTGGCTTACAACTTTTTGTTTCTTCCTCTTTACTGGTTTTGTTGTTTGGGTTCTTGAACATCGAATTAATGAAGACTTTCGTGGTCCTCCTTCACATCAATTTGGGACTAGTGTCTGGTTTTCCTTCTCAACTATGGTTTTCTCACAAAAAGAGAATGTGATTAGCAACTTGACTAGATTCGTGATGATTATATGGGTATTCGTTGTCCTAATATTGACACAAAGTTATACAGCTAATCTAGCATCGCTATTAACAGTCCAACAACTTAAGCCAACTGTTGTTGATATTAAGGATCTTTTAAGGAAGGGGGATAATGTTGGGTACTCTAAGAATGCTTATGTTTATGGACTCTTGAAAGAAGTAGGTTTCAACGATTCAAATCTTAAAGGTTTTAGAACTATGGAAGCACTTGATGAAGCTCTTTCAAAAGGGAGTGCAAATGGTGGTATTGCTGCTTTTGTTGATGAAACCCCAACTATGAAGCTTTTTCTTGCAAAGTATTGTTCTAAATACACCATGATTGGACCCATCTTTAAAACTGAAGGGTTTGGTTTTGTGTTTCCAAAGCGTTCCCCTCTTGTACCTGATGTTTCACAAGCAATCCTAGACGTAACTGAAGGAGAGAAGATGATGAGCATTGAATCAAAATGGTTCAAGAAAGAAAGCAATTGTCAAAGCTCCAGCCAACTGGGTTCTAGCAATAGTCTTGGACTCGATAGCTTTTGGGGGTTATTCCTCATTGCTGGAGTGGCTTCAATATTCGCTCTCATCATATTTGTAGCTTCATTTCTTTATGAGAATGGACACCTATTGATAGATCCTGATTCAGAAGCTTCGACATCAGAAAGGATTCGGGTCTTGTTCAAAATTTTCAATCAGAAAGACCTAAATTCTCGTACATTTAAGAGCAGTCAACCGAGAGATCGTATCACTGGTGCTAGAGATGAAGTAAACATCTCACCAAATTACAACTGGCCAGAAAGTCCATTTAGCTACAGCAATCACAGTGATGGCAGTTCAGAGCAACTCACACCACCTCCAGGTCAAGCATCTTCAGAATCAGTTCCAAGGCATGGATTCCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

940

Amino Acids

104.21

Weight (kDa)

7.21

Isoelectric Point (pI)

29.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peripla_BP_6 PF13458 34 - 324 4.2e-14 Periplasmic binding protein
ANF_receptor PF01094 40 - 389 1.1e-82 Receptor family ligand binding region
Lig_chan-Glu_bd PF10613 468 - 558 1.2e-07 Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3 PF00497 477 - 798 2.2e-07 Bacterial extracellular solute-binding proteins, family 3
Lig_chan PF00060 576 - 830 2e-40 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 599, 1035, 1542
AccB1I GGYRCC 1 cut(s) 1672
AccB7I CCANNNNNTGG 2 cut(s) 1337, 2810
AccIII TCCGGA 1 cut(s) 375
AciI CCGC 4 cut(s) 47, 90, 224, 1199
AclWI GGATC 6 cut(s) 243, 734, 980, 1400, 2037, 2549
AcoI YGGCCR 1 cut(s) 2711
AcsI RAATTY 8 cut(s) 448, 757, 766, 842, 1297, 1385, 2604, 2626
AcuI CTGAAG 7 cut(s) 756, 905, 1086, 1115, 2298, 2373, 2775
AfaI GTAC 8 cut(s) 110, 471, 979, 1473, 1625, 2060, 2319, 2635
AfiI CCNNNNNNNGG 5 cut(s) 85, 1337, 1415, 2777, 2810
AflII CTTAAG 1 cut(s) 2003
AhlI ACTAGT 2 cut(s) 1526, 1844
AjnI CCWGG 2 cut(s) 1291, 2776
Alw21I GWGCWC 1 cut(s) 852
Alw26I GTCTC 4 cut(s) 309, 454, 1045, 1098
AlwI GGATC 6 cut(s) 243, 734, 980, 1400, 2037, 2549
AlwNI CAGNNNCTG 2 cut(s) 584, 1655
Ama87I CYCGRG 1 cut(s) 820
Aor13HI TCCGGA 1 cut(s) 375
AoxI GGCC 4 cut(s) 129, 272, 1289, 2711
ApeKI GCWGC 6 cut(s) 218, 221, 227, 935, 1460, 2189
ApoI RAATTY 8 cut(s) 448, 757, 766, 842, 1297, 1385, 2604, 2626
AseI ATTAAT 2 cut(s) 617, 1803
Asp700I GAANNNNTTC 3 cut(s) 1812, 2586, 2799
AspS9I GGNCC 8 cut(s) 130, 272, 1052, 1277, 1617, 1732, 1820, 2261
AsuHPI GGTGA 4 cut(s) 599, 1178, 1225, 2688
AvaI CYCGRG 1 cut(s) 820
AvaII GGWCC 6 cut(s) 1052, 1277, 1617, 1732, 1820, 2261
BaeGI GKGCMC 1 cut(s) 333
BalI TGGCCA 1 cut(s) 2713
BanI GGYRCC 1 cut(s) 1672
BanII GRGCYC 2 cut(s) 383, 852
BbsI GAAGAC 3 cut(s) 101, 631, 1815
Bbv12I GWGCWC 1 cut(s) 852
BbvI GCAGC 6 cut(s) 208, 230, 239, 922, 1447, 2176
BccI CCATC 8 cut(s) 24, 450, 583, 731, 1314, 1514, 2273, 2741
BceAI ACGGC 2 cut(s) 935, 1568
BciT130I CCWGG 2 cut(s) 1293, 2778
BciVI GTATCC 1 cut(s) 994
BcoDI GTCTC 4 cut(s) 309, 454, 1045, 1098
BcuI ACTAGT 2 cut(s) 1526, 1844
BfmI CTRYAG 1 cut(s) 2731
BfrI CTTAAG 1 cut(s) 2003
BfuI GTATCC 1 cut(s) 994
BglII AGATCT 1 cut(s) 1395
BisI GCNGC 7 cut(s) 219, 222, 225, 228, 936, 1461, 2190
BlsI GCNGC 7 cut(s) 220, 223, 226, 229, 937, 1462, 2191
BmcAI AGTACT 1 cut(s) 1473
Bme1390I CCNGG 2 cut(s) 1293, 2778
Bme18I GGWCC 6 cut(s) 1052, 1277, 1617, 1732, 1820, 2261
BmeT110I CYCGRG 1 cut(s) 820
BmgT120I GGNCC 8 cut(s) 130, 272, 1052, 1277, 1617, 1732, 1820, 2261
BmiI GGNNCC 4 cut(s) 380, 1674, 1733, 2263
BmrFI CCNGG 2 cut(s) 1293, 2778
BmrI ACTGGG 2 cut(s) 1443, 2435
BmsI GCATC 9 cut(s) 158, 359, 514, 532, 922, 1254, 1570, 1988, 2795
BmuI ACTGGG 2 cut(s) 1443, 2435
BoxI GACNNNNGTC 1 cut(s) 1847
BpiI GAAGAC 3 cut(s) 101, 631, 1815
BpmI CTGGAG 4 cut(s) 1122, 2401, 2502, 2760
BpuEI CTTGAG 1 cut(s) 133
BsaAI YACGTR 2 cut(s) 481, 1627
BsaBI GATNNNNATC 1 cut(s) 2115
BsaI GGTCTC 1 cut(s) 1045
BsaJI CCNNGG 4 cut(s) 684, 786, 1313, 2804
BsaWI WCCGGW 2 cut(s) 375, 852
BsaXI ACNNNNNCTCC 4 cut(s) 1350, 1380, 2758, 2788
Bsc4I CCNNNNNNNGG 5 cut(s) 85, 1337, 1415, 2777, 2810
Bse118I RCCGGY 2 cut(s) 8, 1415
Bse1I ACTGG 7 cut(s) 589, 1181, 1438, 1774, 2430, 2674, 2714
Bse3DI GCAATG 1 cut(s) 2475
Bse8I GATNNNNATC 1 cut(s) 2115
BseAI TCCGGA 1 cut(s) 375
BseBI CCWGG 2 cut(s) 1293, 2778
BseDI CCNNGG 4 cut(s) 684, 786, 1313, 2804
BseGI GGATG 8 cut(s) 56, 154, 461, 816, 913, 1245, 1325, 1432
BseJI GATNNNNATC 1 cut(s) 2115
BseLI CCNNNNNNNGG 5 cut(s) 85, 1337, 1415, 2777, 2810
BseMI GCAATG 1 cut(s) 2475
BseMII CTCAG 1 cut(s) 150
BseNI ACTGG 7 cut(s) 589, 1181, 1438, 1774, 2430, 2674, 2714
BseSI GKGCMC 1 cut(s) 333
BseXI GCAGC 6 cut(s) 208, 230, 239, 922, 1447, 2176
BsgI GTGCAG 1 cut(s) 237
BshFI GGCC 4 cut(s) 131, 274, 1291, 2713
BshNI GGYRCC 1 cut(s) 1672
BsiHKAI GWGCWC 1 cut(s) 852
BsiHKCI CYCGRG 1 cut(s) 820
BsiSI CCGG 4 cut(s) 9, 376, 853, 1416
BslFI GGGAC 3 cut(s) 536, 1745, 1855
BslI CCNNNNNNNGG 5 cut(s) 85, 1337, 1415, 2777, 2810
BsmAI GTCTC 4 cut(s) 309, 454, 1045, 1098
BsmBI CGTCTC 1 cut(s) 309
BsmFI GGGAC 3 cut(s) 536, 1745, 1855
BsmI GAATGC 4 cut(s) 49, 253, 1705, 2074
BsnI GGCC 4 cut(s) 131, 274, 1291, 2713
Bso31I GGTCTC 1 cut(s) 1045
BsoBI CYCGRG 1 cut(s) 820
Bsp1286I GDGCHC 3 cut(s) 333, 383, 852
Bsp13I TCCGGA 1 cut(s) 375
Bsp143I GATC 8 cut(s) 235, 726, 972, 1395, 1405, 2029, 2554, 2659
Bsp19I CCATGG 1 cut(s) 786
BspACI CCGC 4 cut(s) 47, 90, 224, 1199
BspANI GGCC 4 cut(s) 131, 274, 1291, 2713
BspCNI CTCAG 1 cut(s) 149
BspEI TCCGGA 1 cut(s) 375
BspLI GGNNCC 4 cut(s) 380, 1674, 1733, 2263
BspPI GGATC 6 cut(s) 243, 734, 980, 1400, 2037, 2549
BspQI GCTCTTC 1 cut(s) 1491
BspT107I GGYRCC 1 cut(s) 1672
BspTI CTTAAG 1 cut(s) 2003
BspTNI GGTCTC 1 cut(s) 1045
BsrDI GCAATG 1 cut(s) 2475
BsrFI RCCGGY 2 cut(s) 8, 1415
BsrI ACTGG 7 cut(s) 589, 1181, 1438, 1774, 2430, 2674, 2714
BssAI RCCGGY 2 cut(s) 8, 1415
BssECI CCNNGG 4 cut(s) 684, 786, 1313, 2804
BssMI GATC 8 cut(s) 235, 726, 972, 1395, 1405, 2029, 2554, 2659
BssT1I CCWWGG 4 cut(s) 684, 786, 1313, 2804
Bst2UI CCWGG 2 cut(s) 1293, 2778
Bst4CI ACNGT 5 cut(s) 208, 560, 1993, 2014, 2744
Bst6I CTCTTC 2 cut(s) 67, 1491
BstAFI CTTAAG 1 cut(s) 2003
BstBAI YACGTR 2 cut(s) 481, 1627
BstC8I GCNNGC 4 cut(s) 291, 295, 1116, 1534
BstDEI CTNAG 2 cut(s) 136, 2064
BstDSI CCRYGG 1 cut(s) 786
BstEII GGTNACC 1 cut(s) 587
BstF5I GGATG 8 cut(s) 56, 154, 461, 816, 913, 1245, 1325, 1432
BstKTI GATC 8 cut(s) 238, 729, 975, 1398, 1408, 2032, 2557, 2662
BstMAI GTCTC 4 cut(s) 309, 454, 1045, 1098
BstMBI GATC 8 cut(s) 235, 726, 972, 1395, 1405, 2029, 2554, 2659
BstMWI GCNNNNNNNGC 7 cut(s) 224, 227, 923, 932, 941, 1976, 2486
BstNI CCWGG 2 cut(s) 1293, 2778
BstPAI GACNNNNGTC 1 cut(s) 1847
BstPI GGTNACC 1 cut(s) 587
BstSCI CCNGG 2 cut(s) 1291, 2776
BstSFI CTRYAG 1 cut(s) 2731
BstSLI GKGCMC 1 cut(s) 333
BstV1I GCAGC 6 cut(s) 208, 230, 239, 922, 1447, 2176
BstV2I GAAGAC 3 cut(s) 101, 631, 1815
BstX2I RGATCY 6 cut(s) 235, 726, 1395, 1405, 2029, 2554
BstXI CCANNNNNNTGG 3 cut(s) 1287, 1320, 2425
BstYI RGATCY 6 cut(s) 235, 726, 1395, 1405, 2029, 2554
BsuI GTATCC 1 cut(s) 994
BsuRI GGCC 4 cut(s) 131, 274, 1291, 2713
BtgI CCRYGG 1 cut(s) 786
BtgZI GCGATG 1 cut(s) 1965
BtsCI GGATG 8 cut(s) 56, 154, 461, 816, 913, 1245, 1325, 1432
BtsIMutI CAGTG 3 cut(s) 1431, 2667, 2749
Cac8I GCNNGC 4 cut(s) 291, 295, 1116, 1534
CaiI CAGNNNCTG 2 cut(s) 584, 1655
Cfr10I RCCGGY 2 cut(s) 8, 1415
Cfr13I GGNCC 8 cut(s) 130, 272, 1052, 1277, 1617, 1732, 1820, 2261
Csp6I GTAC 8 cut(s) 109, 470, 978, 1472, 1624, 2059, 2318, 2634
CspCI CAANNNNNGTGG 2 cut(s) 462, 497
CviAII CATG 7 cut(s) 326, 548, 787, 1457, 1704, 2254, 2810
CviQI GTAC 8 cut(s) 109, 470, 978, 1472, 1624, 2059, 2318, 2634
DdeI CTNAG 2 cut(s) 136, 2064
DpnI GATC 8 cut(s) 237, 728, 974, 1397, 1407, 2031, 2556, 2661
DpnII GATC 8 cut(s) 235, 726, 972, 1395, 1405, 2029, 2554, 2659
DraI TTTAAA 2 cut(s) 756, 2272
EaeI YGGCCR 1 cut(s) 2711
Eam1104I CTCTTC 2 cut(s) 67, 1491
EarI CTCTTC 2 cut(s) 67, 1491
Ecl136II GAGCTC 1 cut(s) 850
Eco130I CCWWGG 4 cut(s) 684, 786, 1313, 2804
Eco24I GRGCYC 2 cut(s) 383, 852
Eco31I GGTCTC 1 cut(s) 1045
Eco47I GGWCC 6 cut(s) 1052, 1277, 1617, 1732, 1820, 2261
Eco53kI GAGCTC 1 cut(s) 850
Eco57I CTGAAG 7 cut(s) 756, 905, 1086, 1115, 2298, 2373, 2775
Eco88I CYCGRG 1 cut(s) 820
Eco91I GGTNACC 1 cut(s) 587
EcoICRI GAGCTC 1 cut(s) 850
EcoO109I RGGNCCY 3 cut(s) 272, 1617, 1732
EcoO65I GGTNACC 1 cut(s) 587
EcoRI GAATTC 1 cut(s) 766
EcoRII CCWGG 2 cut(s) 1291, 2776
EcoT14I CCWWGG 4 cut(s) 684, 786, 1313, 2804
EcoT22I ATGCAT 2 cut(s) 151, 1705
EcoT38I GRGCYC 2 cut(s) 383, 852
ErhI CCWWGG 4 cut(s) 684, 786, 1313, 2804
Esp3I CGTCTC 1 cut(s) 309
FaeI CATG 7 cut(s) 329, 551, 790, 1460, 1707, 2257, 2813
FalI AAGNNNNNCTT 6 cut(s) 95, 127, 1113, 1145, 1551, 1583
FaqI GGGAC 3 cut(s) 536, 1745, 1855
FatI CATG 7 cut(s) 325, 547, 786, 1456, 1703, 2253, 2809
FauI CCCGC 1 cut(s) 83
FauNDI CATATG 1 cut(s) 651
Fnu4HI GCNGC 7 cut(s) 219, 222, 225, 228, 936, 1461, 2190
FokI GGATG 8 cut(s) 43, 141, 468, 803, 920, 1232, 1332, 1439
FriOI GRGCYC 2 cut(s) 383, 852
Fsp4HI GCNGC 7 cut(s) 219, 222, 225, 228, 936, 1461, 2190
GluI GCNGC 7 cut(s) 219, 222, 225, 228, 936, 1461, 2190
GsuI CTGGAG 4 cut(s) 1122, 2401, 2502, 2760
HaeIII GGCC 4 cut(s) 131, 274, 1291, 2713
HapII CCGG 4 cut(s) 9, 376, 853, 1416
Hin1II CATG 7 cut(s) 329, 551, 790, 1460, 1707, 2257, 2813
HincII GTYRAC 1 cut(s) 2651
HindII GTYRAC 1 cut(s) 2651
HindIII AAGCTT 3 cut(s) 1112, 2219, 2568
HpaII CCGG 4 cut(s) 9, 376, 853, 1416
HphI GGTGA 4 cut(s) 599, 1178, 1225, 2688
Hpy166II GTNNAC 5 cut(s) 109, 484, 1630, 2651, 2689
Hpy188I TCNGA 9 cut(s) 139, 240, 988, 1045, 2566, 2581, 2616, 2758, 2794
Hpy8I GTNNAC 5 cut(s) 109, 484, 1630, 2651, 2689
Hpy99I CGWCG 1 cut(s) 1589
HpyCH4III ACNGT 5 cut(s) 208, 560, 1993, 2014, 2744
HpyCH4IV ACGT 3 cut(s) 480, 1626, 2346
HpyF10VI GCNNNNNNNGC 7 cut(s) 224, 227, 923, 932, 941, 1976, 2486
HpyF3I CTNAG 2 cut(s) 136, 2064
HpySE526I ACGT 3 cut(s) 480, 1626, 2346
Hsp92II CATG 7 cut(s) 329, 551, 790, 1460, 1707, 2257, 2813
Kpn2I TCCGGA 1 cut(s) 375
Kzo9I GATC 8 cut(s) 235, 726, 972, 1395, 1405, 2029, 2554, 2659
LguI GCTCTTC 1 cut(s) 1491
LmnI GCTCC 5 cut(s) 378, 378, 855, 1507, 2420
Lsp1109I GCAGC 6 cut(s) 208, 230, 239, 922, 1447, 2176
LweI GCATC 9 cut(s) 158, 359, 514, 532, 922, 1254, 1570, 1988, 2795
MaeII ACGT 3 cut(s) 480, 1626, 2346
MaeIII GTNAC 3 cut(s) 587, 1429, 2347
MalI GATC 8 cut(s) 237, 728, 974, 1397, 1407, 2031, 2556, 2661
MboI GATC 8 cut(s) 235, 726, 972, 1395, 1405, 2029, 2554, 2659
MboII GAAGA 9 cut(s) 54, 106, 631, 762, 1478, 1750, 1820, 2374, 2781
MfeI CAATTG 4 cut(s) 902, 957, 1149, 2404
MflI RGATCY 6 cut(s) 235, 726, 1395, 1405, 2029, 2554
MhlI GDGCHC 3 cut(s) 333, 383, 852
MlsI TGGCCA 1 cut(s) 2713
MluNI TGGCCA 1 cut(s) 2713
MlyI GAGTC 6 cut(s) 404, 645, 1189, 1223, 2079, 2442
MmeI TCCRAC 3 cut(s) 895, 1570, 2020
Mox20I TGGCCA 1 cut(s) 2713
Mph1103I ATGCAT 2 cut(s) 151, 1705
MroI TCCGGA 1 cut(s) 375
MroXI GAANNNNTTC 3 cut(s) 1812, 2586, 2799
MscI TGGCCA 1 cut(s) 2713
MslI CAYNNNNRTG 2 cut(s) 125, 2745
Msp20I TGGCCA 1 cut(s) 2713
MspA1I CMGCKG 1 cut(s) 221
MspCI CTTAAG 1 cut(s) 2003
MspI CCGG 4 cut(s) 9, 376, 853, 1416
MspR9I CCNGG 2 cut(s) 1293, 2778
MunI CAATTG 4 cut(s) 902, 957, 1149, 2404
Mva1269I GAATGC 4 cut(s) 49, 253, 1705, 2074
MvaI CCWGG 2 cut(s) 1293, 2778
MwoI GCNNNNNNNGC 7 cut(s) 224, 227, 923, 932, 941, 1976, 2486
NcoI CCATGG 1 cut(s) 786
NdeI CATATG 1 cut(s) 651
NdeII GATC 8 cut(s) 235, 726, 972, 1395, 1405, 2029, 2554, 2659
NlaIII CATG 7 cut(s) 329, 551, 790, 1460, 1707, 2257, 2813
NlaIV GGNNCC 4 cut(s) 380, 1674, 1733, 2263
NmuCI GTSAC 2 cut(s) 587, 1429
NsiI ATGCAT 2 cut(s) 151, 1705
PciSI GCTCTTC 1 cut(s) 1491
PctI GAATGC 4 cut(s) 49, 253, 1705, 2074
PdmI GAANNNNTTC 3 cut(s) 1812, 2586, 2799
PflMI CCANNNNNTGG 2 cut(s) 1337, 2810
PkrI GCNGC 7 cut(s) 220, 223, 226, 229, 937, 1462, 2191
PleI GAGTC 6 cut(s) 404, 644, 1189, 1223, 2079, 2442
PpsI GAGTC 6 cut(s) 404, 644, 1189, 1223, 2079, 2442
Ppu21I YACGTR 2 cut(s) 481, 1627
PpuMI RGGWCCY 2 cut(s) 1617, 1732
PshAI GACNNNNGTC 1 cut(s) 1847
PshBI ATTAAT 2 cut(s) 617, 1803
PsiI TTATAA 3 cut(s) 599, 1035, 1542
Psp124BI GAGCTC 1 cut(s) 852
Psp5II RGGWCCY 2 cut(s) 1617, 1732
Psp6I CCWGG 2 cut(s) 1291, 2776
PspEI GGTNACC 1 cut(s) 587
PspGI CCWGG 2 cut(s) 1291, 2776
PspN4I GGNNCC 4 cut(s) 380, 1674, 1733, 2263
PspPI GGNCC 8 cut(s) 130, 272, 1052, 1277, 1617, 1732, 1820, 2261
PspPPI RGGWCCY 2 cut(s) 1617, 1732
PstNI CAGNNNCTG 2 cut(s) 584, 1655
PsuI RGATCY 6 cut(s) 235, 726, 1395, 1405, 2029, 2554
PvuII CAGCTG 1 cut(s) 221
RsaI GTAC 8 cut(s) 110, 471, 979, 1473, 1625, 2060, 2319, 2635
RsaNI GTAC 8 cut(s) 109, 470, 978, 1472, 1624, 2059, 2318, 2634
RseI CAYNNNNRTG 2 cut(s) 125, 2745
SacI GAGCTC 1 cut(s) 852
SapI GCTCTTC 1 cut(s) 1491
SatI GCNGC 7 cut(s) 219, 222, 225, 228, 936, 1461, 2190
Sau3AI GATC 8 cut(s) 235, 726, 972, 1395, 1405, 2029, 2554, 2659
Sau96I GGNCC 8 cut(s) 130, 272, 1052, 1277, 1617, 1732, 1820, 2261
ScaI AGTACT 1 cut(s) 1473
SchI GAGTC 6 cut(s) 404, 645, 1189, 1223, 2079, 2442
ScrFI CCNGG 2 cut(s) 1293, 2778
SduI GDGCHC 3 cut(s) 333, 383, 852
SfaNI GCATC 9 cut(s) 158, 359, 514, 532, 922, 1254, 1570, 1988, 2795
SfcI CTRYAG 1 cut(s) 2731
SinI GGWCC 6 cut(s) 1052, 1277, 1617, 1732, 1820, 2261
SmiMI CAYNNNNRTG 2 cut(s) 125, 2745
SmlI CTYRAG 2 cut(s) 112, 2003
SmoI CTYRAG 2 cut(s) 112, 2003
SpeI ACTAGT 2 cut(s) 1526, 1844
SsiI CCGC 4 cut(s) 47, 90, 224, 1199
SspI AATATT 3 cut(s) 267, 1950, 2496
SstI GAGCTC 1 cut(s) 852
StyD4I CCNGG 2 cut(s) 1291, 2776
StyI CCWWGG 4 cut(s) 684, 786, 1313, 2804
TaaI ACNGT 5 cut(s) 208, 560, 1993, 2014, 2744
TaiI ACGT 3 cut(s) 483, 1629, 2349
TaqI TCGA 6 cut(s) 761, 846, 1400, 1798, 2451, 2573
TaqII GACCGA 1 cut(s) 749
TatI WGTACW 3 cut(s) 108, 977, 1471
TauI GCSGC 1 cut(s) 227
TscAI CASTG 3 cut(s) 1438, 2674, 2749
TseFI GTSAC 2 cut(s) 587, 1429
TseI GCWGC 6 cut(s) 218, 221, 227, 935, 1460, 2189
Tsp45I GTSAC 2 cut(s) 587, 1429
TspRI CASTG 3 cut(s) 1438, 2674, 2749
Van91I CCANNNNNTGG 2 cut(s) 1337, 2810
Vha464I CTTAAG 1 cut(s) 2003
VpaK11BI GGWCC 6 cut(s) 1052, 1277, 1617, 1732, 1820, 2261
VspI ATTAAT 2 cut(s) 617, 1803
XapI RAATTY 8 cut(s) 448, 757, 766, 842, 1297, 1385, 2604, 2626
XcmI CCANNNNNNNNNTGG 3 cut(s) 784, 1188, 2706
XmnI GAANNNNTTC 3 cut(s) 1812, 2586, 2799
ZrmI AGTACT 1 cut(s) 1473
Zsp2I ATGCAT 2 cut(s) 151, 1705
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.