Rmu_sc0000725.1_g000014

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000725.1
OG0002036
Physical Location & Seq
Reverse (-)
72793 .. 73104
312 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000725.1_g000014.1.cds

Sequence Viewer

Length: 312 bp
atggggcctttgcatgagggactcagtaggatgtacaagctagcctccgacactcagttcaaatatcttcgggatcaaaagatcattgatccgaaagccttggcggaagcaaaagaggtcgaaaggaagagaaagttggatcagatcaaggcggctgcgaaggaacgtgttgatggccaagcacccgagactgaggttgatgggcaagaggggagtgtgtcagagcatgacgatgattccgaaacaaacacaactaccttcgcctctgcaaaggaattaaaattgttcaagaattactcgtatgccagatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

103

Amino Acids

11.66

Weight (kDa)

5.72

Isoelectric Point (pI)

18.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 104, 152
AclWI GGATC 3 cut(s) 81, 83, 147
AcoI YGGCCR 1 cut(s) 175
AfaI GTAC 1 cut(s) 35
AflIII ACRYGT 1 cut(s) 166
AgsI TTSAA 2 cut(s) 61, 289
AjuI GAANNNNNNNTTGG 2 cut(s) 119, 151
AluBI AGCT 1 cut(s) 40
AluI AGCT 1 cut(s) 40
Alw26I GTCTC 1 cut(s) 182
AlwI GGATC 3 cut(s) 81, 83, 147
Ama87I CYCGRG 1 cut(s) 185
AoxI GGCC 2 cut(s) 5, 175
ApeKI GCWGC 1 cut(s) 155
AspS9I GGNCC 1 cut(s) 5
AsuNHI GCTAGC 1 cut(s) 40
AvaI CYCGRG 1 cut(s) 185
BalI TGGCCA 1 cut(s) 177
BbvI GCAGC 1 cut(s) 142
BccI CCATC 2 cut(s) 167, 194
BcoDI GTCTC 1 cut(s) 182
BfaI CTAG 1 cut(s) 41
BisI GCNGC 2 cut(s) 153, 156
BlsI GCNGC 2 cut(s) 154, 157
BmeT110I CYCGRG 1 cut(s) 185
BmgT120I GGNCC 1 cut(s) 5
BmiI GGNNCC 1 cut(s) 6
BmtI GCTAGC 1 cut(s) 44
BsaJI CCNNGG 1 cut(s) 99
BseDI CCNNGG 1 cut(s) 99
BseGI GGATG 1 cut(s) 36
BseMII CTCAG 3 cut(s) 37, 68, 183
BseXI GCAGC 1 cut(s) 142
BshFI GGCC 2 cut(s) 7, 177
BsiHKCI CYCGRG 1 cut(s) 185
BslFI GGGAC 1 cut(s) 33
BsmAI GTCTC 1 cut(s) 182
BsmFI GGGAC 1 cut(s) 33
BsnI GGCC 2 cut(s) 7, 177
BsoBI CYCGRG 1 cut(s) 185
Bsp1407I TGTACA 1 cut(s) 33
Bsp143I GATC 5 cut(s) 73, 81, 88, 139, 144
BspACI CCGC 2 cut(s) 104, 152
BspANI GGCC 2 cut(s) 7, 177
BspCNI CTCAG 3 cut(s) 36, 67, 184
BspLI GGNNCC 1 cut(s) 6
BspOI GCTAGC 1 cut(s) 44
BspPI GGATC 3 cut(s) 81, 83, 147
BsrGI TGTACA 1 cut(s) 33
BssECI CCNNGG 1 cut(s) 99
BssMI GATC 5 cut(s) 73, 81, 88, 139, 144
BssT1I CCWWGG 1 cut(s) 99
Bst6I CTCTTC 1 cut(s) 122
BstAUI TGTACA 1 cut(s) 33
BstC8I GCNNGC 1 cut(s) 42
BstDEI CTNAG 3 cut(s) 23, 54, 192
BstF5I GGATG 1 cut(s) 36
BstKTI GATC 5 cut(s) 76, 84, 91, 142, 147
BstMAI GTCTC 1 cut(s) 182
BstMBI GATC 5 cut(s) 73, 81, 88, 139, 144
BstV1I GCAGC 1 cut(s) 142
BsuRI GGCC 2 cut(s) 7, 177
BtsCI GGATG 1 cut(s) 36
Cac8I GCNNGC 1 cut(s) 42
Cfr13I GGNCC 1 cut(s) 5
Csp6I GTAC 1 cut(s) 34
CviAII CATG 2 cut(s) 14, 227
CviJI RGCY 6 cut(s) 7, 40, 44, 98, 155, 177
CviKI_1 RGCY 6 cut(s) 7, 40, 44, 98, 155, 177
CviQI GTAC 1 cut(s) 34
DdeI CTNAG 3 cut(s) 23, 54, 192
DpnI GATC 5 cut(s) 75, 83, 90, 141, 146
DpnII GATC 5 cut(s) 73, 81, 88, 139, 144
EaeI YGGCCR 1 cut(s) 175
Eam1104I CTCTTC 1 cut(s) 122
EarI CTCTTC 1 cut(s) 122
EciI GGCGGA 1 cut(s) 119
Eco130I CCWWGG 1 cut(s) 99
Eco88I CYCGRG 1 cut(s) 185
EcoO109I RGGNCCY 1 cut(s) 5
EcoT14I CCWWGG 1 cut(s) 99
ErhI CCWWGG 1 cut(s) 99
FaeI CATG 2 cut(s) 17, 230
FaiI YATR 3 cut(s) 15, 228, 303
FaqI GGGAC 1 cut(s) 33
FatI CATG 2 cut(s) 13, 226
Fnu4HI GCNGC 2 cut(s) 153, 156
FokI GGATG 1 cut(s) 43
Fsp4HI GCNGC 2 cut(s) 153, 156
FspBI CTAG 1 cut(s) 41
GluI GCNGC 2 cut(s) 153, 156
HaeIII GGCC 2 cut(s) 7, 177
Hin1II CATG 2 cut(s) 17, 230
HinfI GANTC 2 cut(s) 21, 236
Hpy188I TCNGA 5 cut(s) 49, 93, 144, 223, 241
Hpy188III TCNNGA 2 cut(s) 71, 289
HpyAV CCTTC 2 cut(s) 154, 268
HpyCH4IV ACGT 1 cut(s) 166
HpyCH4V TGCA 2 cut(s) 13, 269
HpyF3I CTNAG 3 cut(s) 23, 54, 192
HpySE526I ACGT 1 cut(s) 166
Hsp92II CATG 2 cut(s) 17, 230
Kzo9I GATC 5 cut(s) 73, 81, 88, 139, 144
Lsp1109I GCAGC 1 cut(s) 142
MaeI CTAG 1 cut(s) 41
MaeII ACGT 1 cut(s) 166
MalI GATC 5 cut(s) 75, 83, 90, 141, 146
MboI GATC 5 cut(s) 73, 81, 88, 139, 144
MboII GAAGA 2 cut(s) 59, 139
MlsI TGGCCA 1 cut(s) 177
MluCI AATT 3 cut(s) 275, 281, 292
MluNI TGGCCA 1 cut(s) 177
MlyI GAGTC 1 cut(s) 15
MmeI TCCRAC 2 cut(s) 72, 117
MnlI CCTC 6 cut(s) 10, 55, 109, 187, 202, 274
Mox20I TGGCCA 1 cut(s) 177
MscI TGGCCA 1 cut(s) 177
MseI TTAA 1 cut(s) 278
MslI CAYNNNNRTG 1 cut(s) 231
Msp20I TGGCCA 1 cut(s) 177
NdeII GATC 5 cut(s) 73, 81, 88, 139, 144
NheI GCTAGC 1 cut(s) 40
NlaIII CATG 2 cut(s) 17, 230
NlaIV GGNNCC 1 cut(s) 6
PcsI WCGNNNNNNNCGW 1 cut(s) 237
PfeI GAWTC 1 cut(s) 236
PkrI GCNGC 2 cut(s) 154, 157
PleI GAGTC 1 cut(s) 15
PpsI GAGTC 1 cut(s) 15
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 5
RsaI GTAC 1 cut(s) 35
RsaNI GTAC 1 cut(s) 34
RseI CAYNNNNRTG 1 cut(s) 231
SaqAI TTAA 1 cut(s) 278
SatI GCNGC 2 cut(s) 153, 156
Sau3AI GATC 5 cut(s) 73, 81, 88, 139, 144
Sau96I GGNCC 1 cut(s) 5
SchI GAGTC 1 cut(s) 15
SetI ASST 5 cut(s) 42, 120, 169, 198, 260
SmiMI CAYNNNNRTG 1 cut(s) 231
Sse9I AATT 3 cut(s) 275, 281, 292
SsiI CCGC 2 cut(s) 104, 152
SspMI CTAG 1 cut(s) 41
StyI CCWWGG 1 cut(s) 99
TaiI ACGT 1 cut(s) 169
TaqI TCGA 1 cut(s) 120
TasI AATT 3 cut(s) 275, 281, 292
TatI WGTACW 1 cut(s) 33
TauI GCSGC 1 cut(s) 155
TfiI GAWTC 1 cut(s) 236
Tru1I TTAA 1 cut(s) 278
Tru9I TTAA 1 cut(s) 278
TseI GCWGC 1 cut(s) 155
XspI CTAG 1 cut(s) 41
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.