Rh4DG100900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0002036
Physical Location & Seq
Reverse (-)
15309664 .. 15309933
270 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG100900.1

Sequence Viewer

Length: 270 bp
ATGGAATGGTTGGCATCGTTGGACCTTGTAGCCACTGACGCCGGGAAGGTGGTGGTGGATGATGCCATCCGGGATGTACGGATTGAAGAGGGTTTGCACCAGATCAAGCGGCCTAAGTCCGCGGATCTGAAGGATCCGATTCCGGAGGAGGTCGCGAAAAGGCATAAGAAGAAGAATAAAAAGATGGTGACTATGGCTGTGATCGAGGAGAAGTCCGTGAAAGGGGATGAAGAAGAGGTGGGGGAAGAAGAGAATGAAGAGGCACGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

89

Amino Acids

10.08

Weight (kDa)

4.99

Isoelectric Point (pI)

61.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 122, 155
AccIII TCCGGA 1 cut(s) 142
AciI CCGC 3 cut(s) 109, 120, 122
AclWI GGATC 3 cut(s) 128, 132, 141
AcuI CTGAAG 1 cut(s) 149
AcyI GRCGYC 1 cut(s) 39
AfaI GTAC 1 cut(s) 78
AfiI CCNNNNNNNGG 1 cut(s) 222
AgsI TTSAA 1 cut(s) 86
AlwI GGATC 3 cut(s) 128, 132, 141
Aor13HI TCCGGA 1 cut(s) 142
AoxI GGCC 1 cut(s) 110
AspS9I GGNCC 1 cut(s) 22
AsuC2I CCSGG 2 cut(s) 43, 71
AsuHPI GGTGA 1 cut(s) 199
AvaII GGWCC 1 cut(s) 22
BamHI GGATCC 1 cut(s) 133
BccI CCATC 2 cut(s) 74, 178
BcnI CCSGG 2 cut(s) 43, 71
BfaI CTAG 1 cut(s) 268
BisI GCNGC 1 cut(s) 110
BlsI GCNGC 1 cut(s) 111
Bme1390I CCNGG 2 cut(s) 43, 71
Bme18I GGWCC 1 cut(s) 22
BmgT120I GGNCC 1 cut(s) 22
BmiI GGNNCC 1 cut(s) 135
BmrFI CCNGG 2 cut(s) 43, 71
BmsI GCATC 2 cut(s) 23, 52
BpuMI CCSGG 2 cut(s) 43, 71
BsaHI GRCGYC 1 cut(s) 39
BsaJI CCNNGG 1 cut(s) 120
BsaWI WCCGGW 1 cut(s) 142
Bsc4I CCNNNNNNNGG 1 cut(s) 222
BseAI TCCGGA 1 cut(s) 142
BseDI CCNNGG 1 cut(s) 120
BseGI GGATG 4 cut(s) 64, 66, 79, 232
BseLI CCNNNNNNNGG 1 cut(s) 222
BseRI GAGGAG 2 cut(s) 161, 221
Bsh1236I CGCG 2 cut(s) 122, 155
BshFI GGCC 1 cut(s) 112
BsiSI CCGG 3 cut(s) 42, 70, 143
BslI CCNNNNNNNGG 1 cut(s) 222
BsnI GGCC 1 cut(s) 112
Bsp13I TCCGGA 1 cut(s) 142
Bsp143I GATC 4 cut(s) 102, 124, 133, 201
Bsp68I TCGCGA 1 cut(s) 155
BspACI CCGC 3 cut(s) 109, 120, 122
BspANI GGCC 1 cut(s) 112
BspEI TCCGGA 1 cut(s) 142
BspFNI CGCG 2 cut(s) 122, 155
BspLI GGNNCC 1 cut(s) 135
BspPI GGATC 3 cut(s) 128, 132, 141
BssECI CCNNGG 1 cut(s) 120
BssMI GATC 4 cut(s) 102, 124, 133, 201
BssNI GRCGYC 1 cut(s) 39
Bst6I CTCTTC 4 cut(s) 81, 228, 243, 252
BstACI GRCGYC 1 cut(s) 39
BstC8I GCNNGC 1 cut(s) 265
BstDEI CTNAG 1 cut(s) 114
BstDSI CCRYGG 1 cut(s) 120
BstF5I GGATG 4 cut(s) 64, 66, 79, 232
BstFNI CGCG 2 cut(s) 122, 155
BstKTI GATC 4 cut(s) 105, 127, 136, 204
BstMBI GATC 4 cut(s) 102, 124, 133, 201
BstMWI GCNNNNNNNGC 1 cut(s) 38
BstSCI CCNGG 2 cut(s) 41, 69
BstUI CGCG 2 cut(s) 122, 155
BstX2I RGATCY 2 cut(s) 124, 133
BstYI RGATCY 2 cut(s) 124, 133
BsuRI GGCC 1 cut(s) 112
BtgI CCRYGG 1 cut(s) 120
BtsCI GGATG 4 cut(s) 64, 66, 79, 232
BtsIMutI CAGTG 1 cut(s) 33
BtuMI TCGCGA 1 cut(s) 155
Cac8I GCNNGC 1 cut(s) 265
Cfr13I GGNCC 1 cut(s) 22
Cfr42I CCGCGG 1 cut(s) 123
CseI GACGC 1 cut(s) 47
Csp6I GTAC 1 cut(s) 77
CviJI RGCY 3 cut(s) 32, 112, 197
CviKI_1 RGCY 3 cut(s) 32, 112, 197
CviQI GTAC 1 cut(s) 77
DdeI CTNAG 1 cut(s) 114
DpnI GATC 4 cut(s) 104, 126, 135, 203
DpnII GATC 4 cut(s) 102, 124, 133, 201
Eam1104I CTCTTC 4 cut(s) 81, 228, 243, 252
EarI CTCTTC 4 cut(s) 81, 228, 243, 252
Eco47I GGWCC 1 cut(s) 22
Eco57I CTGAAG 1 cut(s) 149
FaiI YATR 2 cut(s) 165, 194
Fnu4HI GCNGC 1 cut(s) 110
FokI GGATG 4 cut(s) 53, 71, 86, 239
Fsp4HI GCNGC 1 cut(s) 110
FspBI CTAG 1 cut(s) 268
GluI GCNGC 1 cut(s) 110
HaeIII GGCC 1 cut(s) 112
HapII CCGG 3 cut(s) 42, 70, 143
HgaI GACGC 1 cut(s) 47
Hin1I GRCGYC 1 cut(s) 39
HinfI GANTC 1 cut(s) 139
HpaII CCGG 3 cut(s) 42, 70, 143
HphI GGTGA 1 cut(s) 199
Hpy188I TCNGA 2 cut(s) 129, 138
Hpy188III TCNNGA 2 cut(s) 143, 154
HpyAV CCTTC 2 cut(s) 40, 124
HpyCH4V TGCA 1 cut(s) 97
HpyF10VI GCNNNNNNNGC 1 cut(s) 38
HpyF3I CTNAG 1 cut(s) 114
Hsp92I GRCGYC 1 cut(s) 39
Kpn2I TCCGGA 1 cut(s) 142
KspI CCGCGG 1 cut(s) 123
Kzo9I GATC 4 cut(s) 102, 124, 133, 201
LpnPI CCDG 4 cut(s) 55, 83, 113, 156
LweI GCATC 2 cut(s) 23, 52
MaeI CTAG 1 cut(s) 268
MaeIII GTNAC 1 cut(s) 187
MalI GATC 4 cut(s) 104, 126, 135, 203
MboI GATC 4 cut(s) 102, 124, 133, 201
MboII GAAGA 8 cut(s) 98, 181, 184, 242, 245, 257, 260, 269
MflI RGATCY 2 cut(s) 124, 133
MnlI CCTC 6 cut(s) 82, 139, 142, 199, 229, 253
MroI TCCGGA 1 cut(s) 142
MspA1I CMGCKG 1 cut(s) 122
MspI CCGG 3 cut(s) 42, 70, 143
MspR9I CCNGG 2 cut(s) 43, 71
MvnI CGCG 2 cut(s) 122, 155
MwoI GCNNNNNNNGC 1 cut(s) 38
NciI CCSGG 2 cut(s) 43, 71
NdeII GATC 4 cut(s) 102, 124, 133, 201
NlaIV GGNNCC 1 cut(s) 135
NmuCI GTSAC 1 cut(s) 187
NruI TCGCGA 1 cut(s) 155
PfeI GAWTC 1 cut(s) 139
PfoI TCCNGGA 1 cut(s) 69
PkrI GCNGC 1 cut(s) 111
PspN4I GGNNCC 1 cut(s) 135
PspPI GGNCC 1 cut(s) 22
PsuI RGATCY 2 cut(s) 124, 133
RruI TCGCGA 1 cut(s) 155
RsaI GTAC 1 cut(s) 78
RsaNI GTAC 1 cut(s) 77
SacII CCGCGG 1 cut(s) 123
SatI GCNGC 1 cut(s) 110
Sau3AI GATC 4 cut(s) 102, 124, 133, 201
Sau96I GGNCC 1 cut(s) 22
ScrFI CCNGG 2 cut(s) 43, 71
SetI ASST 4 cut(s) 27, 51, 153, 240
SfaNI GCATC 2 cut(s) 23, 52
Sfr303I CCGCGG 1 cut(s) 123
SgrBI CCGCGG 1 cut(s) 123
SinI GGWCC 1 cut(s) 22
SsiI CCGC 3 cut(s) 109, 120, 122
SspMI CTAG 1 cut(s) 268
StyD4I CCNGG 2 cut(s) 41, 69
TaqI TCGA 1 cut(s) 204
TauI GCSGC 1 cut(s) 112
TfiI GAWTC 1 cut(s) 139
TscAI CASTG 1 cut(s) 40
TseFI GTSAC 1 cut(s) 187
Tsp45I GTSAC 1 cut(s) 187
TspDTI ATGAA 2 cut(s) 243, 270
TspGWI ACGGA 2 cut(s) 94, 205
TspRI CASTG 1 cut(s) 40
VpaK11BI GGWCC 1 cut(s) 22
XspI CTAG 1 cut(s) 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.