Belongs to the BI1 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 328 |
| AccI | GTMKAC | 1 cut(s) | 156 |
| AfaI | GTAC | 1 cut(s) | 42 |
| AgsI | TTSAA | 3 cut(s) | 50, 522, 530 |
| AjnI | CCWGG | 1 cut(s) | 371 |
| AluBI | AGCT | 4 cut(s) | 130, 178, 379, 417 |
| AluI | AGCT | 4 cut(s) | 130, 178, 379, 417 |
| AoxI | GGCC | 2 cut(s) | 106, 545 |
| AsuC2I | CCSGG | 1 cut(s) | 105 |
| AsuHPI | GGTGA | 2 cut(s) | 199, 470 |
| AxyI | CCTNAGG | 1 cut(s) | 357 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 308, 341 |
| BccI | CCATC | 5 cut(s) | 203, 232, 233, 380, 556 |
| BceAI | ACGGC | 1 cut(s) | 175 |
| BciT130I | CCWGG | 1 cut(s) | 373 |
| BcnI | CCSGG | 1 cut(s) | 105 |
| BfaI | CTAG | 1 cut(s) | 306 |
| BfuAI | ACCTGC | 1 cut(s) | 328 |
| Bme1390I | CCNGG | 2 cut(s) | 105, 373 |
| BmrFI | CCNGG | 2 cut(s) | 105, 373 |
| BmrI | ACTGGG | 1 cut(s) | 567 |
| BmsI | GCATC | 2 cut(s) | 10, 171 |
| BmuI | ACTGGG | 1 cut(s) | 567 |
| BpmI | CTGGAG | 1 cut(s) | 403 |
| Bpu10I | CCTNAGC | 1 cut(s) | 453 |
| BpuEI | CTTGAG | 1 cut(s) | 314 |
| BpuMI | CCSGG | 1 cut(s) | 105 |
| BsaJI | CCNNGG | 2 cut(s) | 103, 372 |
| Bse1I | ACTGG | 1 cut(s) | 562 |
| Bse21I | CCTNAGG | 1 cut(s) | 357 |
| BseBI | CCWGG | 1 cut(s) | 373 |
| BseDI | CCNNGG | 2 cut(s) | 103, 372 |
| BseGI | GGATG | 2 cut(s) | 238, 391 |
| BseMII | CTCAG | 4 cut(s) | 124, 188, 348, 444 |
| BseNI | ACTGG | 1 cut(s) | 562 |
| BseRI | GAGGAG | 1 cut(s) | 173 |
| BshFI | GGCC | 2 cut(s) | 108, 547 |
| BsiSI | CCGG | 2 cut(s) | 87, 105 |
| BsnI | GGCC | 2 cut(s) | 108, 547 |
| Bsp143I | GATC | 1 cut(s) | 504 |
| BspANI | GGCC | 2 cut(s) | 108, 547 |
| BspCNI | CTCAG | 4 cut(s) | 123, 187, 349, 445 |
| BspMI | ACCTGC | 1 cut(s) | 328 |
| BsrI | ACTGG | 1 cut(s) | 562 |
| BssECI | CCNNGG | 2 cut(s) | 103, 372 |
| BssMI | GATC | 1 cut(s) | 504 |
| Bst2UI | CCWGG | 1 cut(s) | 373 |
| Bst4CI | ACNGT | 1 cut(s) | 211 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 20 |
| BstDEI | CTNAG | 6 cut(s) | 71, 110, 174, 357, 453, 542 |
| BstF5I | GGATG | 2 cut(s) | 238, 391 |
| BstKTI | GATC | 1 cut(s) | 507 |
| BstMBI | GATC | 1 cut(s) | 504 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 20 |
| BstNI | CCWGG | 1 cut(s) | 373 |
| BstSCI | CCNGG | 2 cut(s) | 103, 371 |
| Bsu36I | CCTNAGG | 1 cut(s) | 357 |
| BsuRI | GGCC | 2 cut(s) | 108, 547 |
| BtgZI | GCGATG | 1 cut(s) | 172 |
| BtsCI | GGATG | 2 cut(s) | 238, 391 |
| BtsI | GCAGTG | 1 cut(s) | 484 |
| BtsIMutI | CAGTG | 2 cut(s) | 451, 484 |
| BveI | ACCTGC | 1 cut(s) | 328 |
| Csp6I | GTAC | 1 cut(s) | 41 |
| CviAII | CATG | 1 cut(s) | 38 |
| CviQI | GTAC | 1 cut(s) | 41 |
| DdeI | CTNAG | 6 cut(s) | 71, 110, 174, 357, 453, 542 |
| DpnI | GATC | 1 cut(s) | 506 |
| DpnII | GATC | 1 cut(s) | 504 |
| Eco81I | CCTNAGG | 1 cut(s) | 357 |
| EcoRII | CCWGG | 1 cut(s) | 371 |
| FaeI | CATG | 1 cut(s) | 41 |
| FaiI | YATR | 8 cut(s) | 39, 146, 289, 291, 339, 341, 497, 577 |
| FatI | CATG | 1 cut(s) | 37 |
| FauNDI | CATATG | 2 cut(s) | 289, 339 |
| FblI | GTMKAC | 1 cut(s) | 156 |
| FokI | GGATG | 2 cut(s) | 245, 398 |
| FspBI | CTAG | 1 cut(s) | 306 |
| GsuI | CTGGAG | 1 cut(s) | 403 |
| HaeIII | GGCC | 2 cut(s) | 108, 547 |
| HapII | CCGG | 2 cut(s) | 87, 105 |
| Hin1II | CATG | 1 cut(s) | 41 |
| HinfI | GANTC | 3 cut(s) | 475, 514, 538 |
| HpaII | CCGG | 2 cut(s) | 87, 105 |
| HphI | GGTGA | 2 cut(s) | 199, 470 |
| Hpy166II | GTNNAC | 2 cut(s) | 157, 369 |
| Hpy188I | TCNGA | 1 cut(s) | 504 |
| Hpy188III | TCNNGA | 3 cut(s) | 331, 356, 565 |
| Hpy8I | GTNNAC | 2 cut(s) | 157, 369 |
| HpyAV | CCTTC | 4 cut(s) | 211, 471, 491, 528 |
| HpyCH4III | ACNGT | 1 cut(s) | 211 |
| HpyCH4V | TGCA | 4 cut(s) | 14, 100, 343, 489 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 20 |
| HpyF3I | CTNAG | 6 cut(s) | 71, 110, 174, 357, 453, 542 |
| Hsp92II | CATG | 1 cut(s) | 41 |
| Kzo9I | GATC | 1 cut(s) | 504 |
| LmnI | GCTCC | 3 cut(s) | 135, 376, 422 |
| LweI | GCATC | 2 cut(s) | 10, 171 |
| MaeI | CTAG | 1 cut(s) | 306 |
| MaeIII | GTNAC | 4 cut(s) | 59, 205, 476, 565 |
| MalI | GATC | 1 cut(s) | 506 |
| MboI | GATC | 1 cut(s) | 504 |
| MboII | GAAGA | 1 cut(s) | 305 |
| MluCI | AATT | 1 cut(s) | 82 |
| MlyI | GAGTC | 2 cut(s) | 484, 508 |
| MnlI | CCTC | 6 cut(s) | 35, 119, 181, 194, 352, 445 |
| MseI | TTAA | 1 cut(s) | 312 |
| MspI | CCGG | 2 cut(s) | 87, 105 |
| MspR9I | CCNGG | 2 cut(s) | 105, 373 |
| MvaI | CCWGG | 1 cut(s) | 373 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 20 |
| NciI | CCSGG | 1 cut(s) | 105 |
| NdeI | CATATG | 2 cut(s) | 289, 339 |
| NdeII | GATC | 1 cut(s) | 504 |
| NlaIII | CATG | 1 cut(s) | 41 |
| NmuCI | GTSAC | 4 cut(s) | 59, 205, 476, 565 |
| PfeI | GAWTC | 1 cut(s) | 538 |
| PleI | GAGTC | 2 cut(s) | 483, 508 |
| PpsI | GAGTC | 2 cut(s) | 483, 508 |
| Psp6I | CCWGG | 1 cut(s) | 371 |
| PspGI | CCWGG | 1 cut(s) | 371 |
| RsaI | GTAC | 1 cut(s) | 42 |
| RsaNI | GTAC | 1 cut(s) | 41 |
| SaqAI | TTAA | 1 cut(s) | 312 |
| Sau3AI | GATC | 1 cut(s) | 504 |
| SchI | GAGTC | 2 cut(s) | 484, 508 |
| ScrFI | CCNGG | 2 cut(s) | 105, 373 |
| SfaNI | GCATC | 2 cut(s) | 10, 171 |
| SmlI | CTYRAG | 1 cut(s) | 329 |
| SmoI | CTYRAG | 1 cut(s) | 329 |
| Sse9I | AATT | 1 cut(s) | 82 |
| SspMI | CTAG | 1 cut(s) | 306 |
| StyD4I | CCNGG | 2 cut(s) | 103, 371 |
| TaaI | ACNGT | 1 cut(s) | 211 |
| TasI | AATT | 1 cut(s) | 82 |
| TfiI | GAWTC | 1 cut(s) | 538 |
| Tru1I | TTAA | 1 cut(s) | 312 |
| Tru9I | TTAA | 1 cut(s) | 312 |
| TscAI | CASTG | 2 cut(s) | 451, 491 |
| TseFI | GTSAC | 4 cut(s) | 59, 205, 476, 565 |
| Tsp45I | GTSAC | 4 cut(s) | 59, 205, 476, 565 |
| TspDTI | ATGAA | 2 cut(s) | 133, 306 |
| TspGWI | ACGGA | 1 cut(s) | 122 |
| TspRI | CASTG | 2 cut(s) | 451, 491 |
| XmiI | GTMKAC | 1 cut(s) | 156 |
| XspI | CTAG | 1 cut(s) | 306 |