Cleavage and polyadenylation specificity factor
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 40 |
| AfaI | GTAC | 1 cut(s) | 131 |
| AflIII | ACRYGT | 2 cut(s) | 50, 145 |
| AgsI | TTSAA | 2 cut(s) | 76, 265 |
| AjiI | CACGTC | 1 cut(s) | 146 |
| AluBI | AGCT | 2 cut(s) | 200, 347 |
| AluI | AGCT | 2 cut(s) | 200, 347 |
| ApoI | RAATTY | 1 cut(s) | 40 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 269 |
| AspS9I | GGNCC | 1 cut(s) | 257 |
| AvaII | GGWCC | 1 cut(s) | 257 |
| BfaI | CTAG | 2 cut(s) | 18, 69 |
| Bme18I | GGWCC | 1 cut(s) | 257 |
| BmgBI | CACGTC | 1 cut(s) | 146 |
| BmgT120I | GGNCC | 1 cut(s) | 257 |
| BmsI | GCATC | 1 cut(s) | 340 |
| Bpu10I | CCTNAGC | 1 cut(s) | 284 |
| BpuEI | CTTGAG | 2 cut(s) | 76, 139 |
| Bse1I | ACTGG | 2 cut(s) | 316, 345 |
| BseNI | ACTGG | 2 cut(s) | 316, 345 |
| Bsh1285I | CGRYCG | 1 cut(s) | 211 |
| BsiEI | CGRYCG | 1 cut(s) | 211 |
| BslFI | GGGAC | 1 cut(s) | 156 |
| BsmFI | GGGAC | 1 cut(s) | 156 |
| Bsp143I | GATC | 1 cut(s) | 208 |
| BsrI | ACTGG | 2 cut(s) | 316, 345 |
| BssMI | GATC | 1 cut(s) | 208 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 102 |
| BstC8I | GCNNGC | 1 cut(s) | 198 |
| BstDEI | CTNAG | 1 cut(s) | 284 |
| BstKTI | GATC | 1 cut(s) | 211 |
| BstMBI | GATC | 1 cut(s) | 208 |
| BstMCI | CGRYCG | 1 cut(s) | 211 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 102 |
| BstNSI | RCATGY | 1 cut(s) | 54 |
| BtrI | CACGTC | 1 cut(s) | 146 |
| Cac8I | GCNNGC | 1 cut(s) | 198 |
| Cfr13I | GGNCC | 1 cut(s) | 257 |
| Csp6I | GTAC | 1 cut(s) | 130 |
| CviAII | CATG | 1 cut(s) | 51 |
| CviJI | RGCY | 6 cut(s) | 17, 110, 200, 283, 347, 403 |
| CviKI_1 | RGCY | 6 cut(s) | 17, 110, 200, 283, 347, 403 |
| CviQI | GTAC | 1 cut(s) | 130 |
| DdeI | CTNAG | 1 cut(s) | 284 |
| DpnI | GATC | 1 cut(s) | 210 |
| DpnII | GATC | 1 cut(s) | 208 |
| Eco47I | GGWCC | 1 cut(s) | 257 |
| EcoRI | GAATTC | 1 cut(s) | 40 |
| EcoT22I | ATGCAT | 1 cut(s) | 298 |
| FaeI | CATG | 1 cut(s) | 54 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 369, 401 |
| FaqI | GGGAC | 1 cut(s) | 156 |
| FatI | CATG | 1 cut(s) | 50 |
| FauNDI | CATATG | 1 cut(s) | 298 |
| FspBI | CTAG | 2 cut(s) | 18, 69 |
| Hin1II | CATG | 1 cut(s) | 54 |
| HinfI | GANTC | 2 cut(s) | 121, 240 |
| Hpy188III | TCNNGA | 3 cut(s) | 76, 118, 171 |
| HpyAV | CCTTC | 1 cut(s) | 259 |
| HpyCH4IV | ACGT | 1 cut(s) | 145 |
| HpyCH4V | TGCA | 4 cut(s) | 105, 296, 302, 353 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 102 |
| HpyF3I | CTNAG | 1 cut(s) | 284 |
| HpySE526I | ACGT | 1 cut(s) | 145 |
| Hsp92II | CATG | 1 cut(s) | 54 |
| Kzo9I | GATC | 1 cut(s) | 208 |
| LpnPI | CCDG | 2 cut(s) | 297, 326 |
| LweI | GCATC | 1 cut(s) | 340 |
| MaeI | CTAG | 2 cut(s) | 18, 69 |
| MaeII | ACGT | 1 cut(s) | 145 |
| MaeIII | GTNAC | 1 cut(s) | 183 |
| MalI | GATC | 1 cut(s) | 210 |
| MboI | GATC | 1 cut(s) | 208 |
| MboII | GAAGA | 1 cut(s) | 68 |
| MluCI | AATT | 3 cut(s) | 40, 315, 331 |
| MlyI | GAGTC | 1 cut(s) | 130 |
| MmeI | TCCRAC | 1 cut(s) | 179 |
| MnlI | CCTC | 3 cut(s) | 16, 173, 206 |
| Mph1103I | ATGCAT | 1 cut(s) | 298 |
| MroXI | GAANNNNTTC | 1 cut(s) | 269 |
| MseI | TTAA | 2 cut(s) | 249, 412 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 102 |
| NdeI | CATATG | 1 cut(s) | 298 |
| NdeII | GATC | 1 cut(s) | 208 |
| NlaIII | CATG | 1 cut(s) | 54 |
| NsiI | ATGCAT | 1 cut(s) | 298 |
| NspI | RCATGY | 1 cut(s) | 54 |
| PciI | ACATGT | 1 cut(s) | 50 |
| PdmI | GAANNNNTTC | 1 cut(s) | 269 |
| PfeI | GAWTC | 1 cut(s) | 240 |
| Ple19I | CGATCG | 1 cut(s) | 211 |
| PleI | GAGTC | 1 cut(s) | 129 |
| PpsI | GAGTC | 1 cut(s) | 129 |
| PscI | ACATGT | 1 cut(s) | 50 |
| PspPI | GGNCC | 1 cut(s) | 257 |
| PvuI | CGATCG | 1 cut(s) | 211 |
| RsaI | GTAC | 1 cut(s) | 131 |
| RsaNI | GTAC | 1 cut(s) | 130 |
| SaqAI | TTAA | 2 cut(s) | 249, 412 |
| Sau3AI | GATC | 1 cut(s) | 208 |
| Sau96I | GGNCC | 1 cut(s) | 257 |
| SchI | GAGTC | 1 cut(s) | 130 |
| SetI | ASST | 5 cut(s) | 131, 148, 202, 217, 349 |
| SfaNI | GCATC | 1 cut(s) | 340 |
| SinI | GGWCC | 1 cut(s) | 257 |
| SmlI | CTYRAG | 2 cut(s) | 91, 118 |
| SmoI | CTYRAG | 2 cut(s) | 91, 118 |
| Sse9I | AATT | 3 cut(s) | 40, 315, 331 |
| SspMI | CTAG | 2 cut(s) | 18, 69 |
| TaiI | ACGT | 1 cut(s) | 148 |
| TaqI | TCGA | 1 cut(s) | 211 |
| TasI | AATT | 3 cut(s) | 40, 315, 331 |
| TfiI | GAWTC | 1 cut(s) | 240 |
| Tru1I | TTAA | 2 cut(s) | 249, 412 |
| Tru9I | TTAA | 2 cut(s) | 249, 412 |
| TspDTI | ATGAA | 2 cut(s) | 351, 407 |
| VpaK11BI | GGWCC | 1 cut(s) | 257 |
| XapI | RAATTY | 1 cut(s) | 40 |
| XceI | RCATGY | 1 cut(s) | 54 |
| XmnI | GAANNNNTTC | 1 cut(s) | 269 |
| XspI | CTAG | 2 cut(s) | 18, 69 |
| Zsp2I | ATGCAT | 1 cut(s) | 298 |