Rorug06G0259600

TMV resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0002558
Physical Location & Seq
Reverse (-)
40657248 .. 40660322
3075 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0259600.1

Sequence Viewer

Length: 1218 bp
ATGAAGAAGACCACCATTTCTATTTCTCAGAAGGACCATAAAAGAAGGAGGTTATTCTTTCGTTCATGTCTCCTTTCTACTCTGCTTATGCTTTCTGCAGTTTTCTTCTGTGGGAGCCATTTTGTTGTCACAGATTACAAAGAGAAGTTTTTATCATGGGGATTGGTTGATGCTATGCACAATGCAATATCTAAAAATTGCGAGGCTCAATGCAGACCTGAGGGAATTGAGGCATTACCTAAAGGAATTGTTTCCAAGACCTCTGACTTTGTAATGCAACCATTATGGGGACCCCGAACTCAAAAGAAACCAAAGTCATCAACAAACTTATTGGCCATTGCAGTTGGAATCAAGCAAAAAGAAAGTGTAAACAAAATTGTAAAGAAGTTTCTATCAAGCGATTTCGTCGTGATGCTATTTCATTATGATGGTAATGTAAATGACTGGAGGGATTTGGAATGGAGTGGTCGGGCCATACATGTATCTGCCATGAATCAAACCAAGTGGTGGTATGCAAAGCGATTCTTACACCCAGATATTGTTTCTGAGTATGCATTCATCTTCCTTTGGGATGAAGACCTTGGAATCGAAAACTTTAATGTTGGAAGATATCTATCAATTATTAGAAAAGAGGGGCTTGAAATTTCACAGCCAGGACTTGATCCTGACAACTCGGAGGTGCATCATGATTTTACAGCAAGAGACAAGAGATCAGAAGTACATAGGAAGATAAACAAGACAATTGGTGGTGGAAGGCGTTGCAATGAAAATAGCACAGATCCTCCATGCACAGGGTTTGTTGAAATGATGGCTCCTGTTTTTTCAAGAGCATCCTGGCACTGCGTGTGGCATATGATTCAGAATGACTTGGTTCATGCATGGGGCTTGGATTTTCAGCTTGGTTATTGTGCACAGGGTGATCGAACCCAAAATATCGGAATTGTTGATTCTGAGTACATTGTTCATTACGGTCTTCCTACACTAGGGGGTTCTGCAGCAAACAAAACAAATGCTGAAGAACAAGATCAAGCTGCTAAAAATATGTCTACTTCAAAACAACTGGCACCATCGCAGTCATCTCCATCTGACCCTAGAACTGAGGTGAGGAAGCTATCATTTGTTGAACTCGAAATTTTCAAAAACAGATGGAAAAAGGCAATTAGAGAAGATAATTGCTGGGTTGATCCATACCAGAAGCCACCACAGCAGAGTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TIR

Protein Analysis

405

Amino Acids

46.34

Weight (kDa)

8.9

Isoelectric Point (pI)

48.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF707 PF05212 70 - 388 5.2e-144 Protein of unknown function (DUF707)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1063
AccB7I CCANNNNNTGG 1 cut(s) 507
AccI GTMKAC 1 cut(s) 1046
AclWI GGATC 3 cut(s) 656, 773, 1178
AcoI YGGCCR 1 cut(s) 333
AcsI RAATTY 2 cut(s) 642, 1131
AcuI CTGAAG 1 cut(s) 1035
AdeI CACNNNGTG 2 cut(s) 844, 917
AfaI GTAC 2 cut(s) 720, 956
AfiI CCNNNNNNNGG 4 cut(s) 287, 507, 791, 983
AflIII ACRYGT 1 cut(s) 478
AgsI TTSAA 6 cut(s) 641, 803, 825, 1053, 1124, 1138
AjnI CCWGG 2 cut(s) 652, 833
AluBI AGCT 3 cut(s) 898, 1031, 1111
AluI AGCT 3 cut(s) 898, 1031, 1111
Alw21I GWGCWC 1 cut(s) 913
Alw26I GTCTC 2 cut(s) 74, 696
Alw44I GTGCAC 1 cut(s) 909
AlwI GGATC 3 cut(s) 656, 773, 1178
AoxI GGCC 2 cut(s) 333, 471
ApaLI GTGCAC 1 cut(s) 909
ApeKI GCWGC 2 cut(s) 995, 1031
ApoI RAATTY 2 cut(s) 642, 1131
Asp700I GAANNNNTTC 1 cut(s) 250
AspS9I GGNCC 3 cut(s) 34, 290, 471
AsuHPI GGTGA 2 cut(s) 929, 1114
AvaII GGWCC 2 cut(s) 34, 290
AxyI CCTNAGG 1 cut(s) 219
BaeGI GKGCMC 1 cut(s) 913
BalI TGGCCA 1 cut(s) 335
BanI GGYRCC 1 cut(s) 1063
BbsI GAAGAC 3 cut(s) 14, 582, 965
Bbv12I GWGCWC 1 cut(s) 913
BbvI GCAGC 2 cut(s) 1007, 1018
BccI CCATC 5 cut(s) 422, 802, 1075, 1090, 1140
BciT130I CCWGG 2 cut(s) 654, 835
BcoDI GTCTC 2 cut(s) 74, 696
BfaI CTAG 2 cut(s) 983, 1092
BfmI CTRYAG 2 cut(s) 96, 993
BisI GCNGC 2 cut(s) 996, 1032
BlsI GCNGC 2 cut(s) 997, 1033
Bme1390I CCNGG 2 cut(s) 654, 835
Bme18I GGWCC 2 cut(s) 34, 290
BmgT120I GGNCC 3 cut(s) 34, 290, 471
BmiI GGNNCC 5 cut(s) 116, 291, 292, 813, 1065
BmrFI CCNGG 2 cut(s) 654, 835
BmsI GCATC 4 cut(s) 160, 402, 691, 839
BpiI GAAGAC 3 cut(s) 14, 582, 965
BpmI CTGGAG 1 cut(s) 466
BsaBI GATNNNNATC 1 cut(s) 613
BsaJI CCNNGG 1 cut(s) 580
BsaXI ACNNNNNCTCC 2 cut(s) 766, 796
Bsc4I CCNNNNNNNGG 4 cut(s) 287, 507, 791, 983
Bse1I ACTGG 2 cut(s) 449, 1065
Bse21I CCTNAGG 1 cut(s) 219
Bse3DI GCAATG 2 cut(s) 336, 769
Bse8I GATNNNNATC 1 cut(s) 613
BseBI CCWGG 2 cut(s) 654, 835
BseDI CCNNGG 1 cut(s) 580
BseGI GGATG 2 cut(s) 577, 830
BseJI GATNNNNATC 1 cut(s) 613
BseLI CCNNNNNNNGG 4 cut(s) 287, 507, 791, 983
BseMI GCAATG 2 cut(s) 336, 769
BseMII CTCAG 5 cut(s) 41, 210, 537, 942, 1089
BseNI ACTGG 2 cut(s) 449, 1065
BseSI GKGCMC 1 cut(s) 913
BseXI GCAGC 2 cut(s) 1007, 1018
BseYI CCCAGC 1 cut(s) 1176
BshFI GGCC 2 cut(s) 335, 473
BshNI GGYRCC 1 cut(s) 1063
BsiHKAI GWGCWC 1 cut(s) 913
BslFI GGGAC 1 cut(s) 303
BslI CCNNNNNNNGG 4 cut(s) 287, 507, 791, 983
BsmAI GTCTC 2 cut(s) 74, 696
BsmFI GGGAC 1 cut(s) 303
BsmI GAATGC 1 cut(s) 554
BsnI GGCC 2 cut(s) 335, 473
Bsp1286I GDGCHC 1 cut(s) 913
Bsp143I GATC 6 cut(s) 661, 710, 778, 919, 1024, 1183
BspANI GGCC 2 cut(s) 335, 473
BspCNI CTCAG 5 cut(s) 40, 211, 538, 943, 1090
BspHI TCATGA 1 cut(s) 685
BspLI GGNNCC 5 cut(s) 116, 291, 292, 813, 1065
BspMAI CTGCAG 2 cut(s) 100, 997
BspPI GGATC 3 cut(s) 656, 773, 1178
BspT107I GGYRCC 1 cut(s) 1063
BsrDI GCAATG 2 cut(s) 336, 769
BsrI ACTGG 2 cut(s) 449, 1065
BssECI CCNNGG 1 cut(s) 580
BssMI GATC 6 cut(s) 661, 710, 778, 919, 1024, 1183
BssT1I CCWWGG 1 cut(s) 580
Bst2UI CCWGG 2 cut(s) 654, 835
Bst4CI ACNGT 1 cut(s) 971
BstDEI CTNAG 5 cut(s) 27, 219, 546, 951, 1098
BstENI CCTNNNNNAGG 1 cut(s) 981
BstF5I GGATG 2 cut(s) 577, 830
BstKTI GATC 6 cut(s) 664, 713, 781, 922, 1027, 1186
BstMAI GTCTC 2 cut(s) 74, 696
BstMBI GATC 6 cut(s) 661, 710, 778, 919, 1024, 1183
BstMWI GCNNNNNNNGC 1 cut(s) 1204
BstNI CCWGG 2 cut(s) 654, 835
BstNSI RCATGY 1 cut(s) 482
BstSCI CCNGG 2 cut(s) 652, 833
BstSFI CTRYAG 2 cut(s) 96, 993
BstSLI GKGCMC 1 cut(s) 913
BstV1I GCAGC 2 cut(s) 1007, 1018
BstV2I GAAGAC 3 cut(s) 14, 582, 965
BstX2I RGATCY 1 cut(s) 778
BstYI RGATCY 1 cut(s) 778
Bsu36I CCTNAGG 1 cut(s) 219
BsuRI GGCC 2 cut(s) 335, 473
BtgZI GCGATG 1 cut(s) 1053
BtsCI GGATG 2 cut(s) 577, 830
BtsI GCAGTG 1 cut(s) 838
BtsIMutI CAGTG 1 cut(s) 838
CciI TCATGA 1 cut(s) 685
Cfr13I GGNCC 3 cut(s) 34, 290, 471
Csp6I GTAC 2 cut(s) 719, 955
CspCI CAANNNNNGTGG 2 cut(s) 485, 520
CviAII CATG 8 cut(s) 66, 156, 479, 490, 686, 786, 875, 879
CviQI GTAC 2 cut(s) 719, 955
DdeI CTNAG 5 cut(s) 27, 219, 546, 951, 1098
DpnI GATC 6 cut(s) 663, 712, 780, 921, 1026, 1185
DpnII GATC 6 cut(s) 661, 710, 778, 919, 1024, 1183
DraIII CACNNNGTG 2 cut(s) 844, 917
EaeI YGGCCR 1 cut(s) 333
Eco130I CCWWGG 1 cut(s) 580
Eco32I GATATC 1 cut(s) 611
Eco47I GGWCC 2 cut(s) 34, 290
Eco57I CTGAAG 1 cut(s) 1035
Eco81I CCTNAGG 1 cut(s) 219
EcoNI CCTNNNNNAGG 1 cut(s) 981
EcoO109I RGGNCCY 1 cut(s) 290
EcoRII CCWGG 2 cut(s) 652, 833
EcoRV GATATC 1 cut(s) 611
EcoT14I CCWWGG 1 cut(s) 580
EcoT22I ATGCAT 2 cut(s) 556, 880
ErhI CCWWGG 1 cut(s) 580
FaeI CATG 8 cut(s) 69, 159, 482, 493, 689, 789, 878, 882
FalI AAGNNNNNCTT 4 cut(s) 509, 541, 621, 653
FaqI GGGAC 1 cut(s) 303
FatI CATG 8 cut(s) 65, 155, 478, 489, 685, 785, 874, 878
FauNDI CATATG 1 cut(s) 852
FblI GTMKAC 1 cut(s) 1046
Fnu4HI GCNGC 2 cut(s) 996, 1032
FokI GGATG 2 cut(s) 584, 817
Fsp4HI GCNGC 2 cut(s) 996, 1032
FspBI CTAG 2 cut(s) 983, 1092
GluI GCNGC 2 cut(s) 996, 1032
GsaI CCCAGC 1 cut(s) 1180
GsuI CTGGAG 1 cut(s) 466
HaeIII GGCC 2 cut(s) 335, 473
Hin1II CATG 8 cut(s) 69, 159, 482, 493, 689, 789, 878, 882
HinfI GANTC 6 cut(s) 348, 493, 522, 585, 856, 947
HphI GGTGA 2 cut(s) 929, 1114
Hpy166II GTNNAC 3 cut(s) 370, 911, 1047
Hpy188I TCNGA 9 cut(s) 30, 265, 547, 676, 715, 861, 938, 952, 1087
Hpy188III TCNNGA 4 cut(s) 409, 665, 686, 825
Hpy8I GTNNAC 3 cut(s) 370, 911, 1047
Hpy99I CGWCG 1 cut(s) 410
HpyAV CCTTC 3 cut(s) 25, 39, 747
HpyCH4III ACNGT 1 cut(s) 971
HpyF10VI GCNNNNNNNGC 1 cut(s) 1204
HpyF3I CTNAG 5 cut(s) 27, 219, 546, 951, 1098
Hsp92II CATG 8 cut(s) 69, 159, 482, 493, 689, 789, 878, 882
KflI GGGWCCC 1 cut(s) 290
Kzo9I GATC 6 cut(s) 661, 710, 778, 919, 1024, 1183
LmnI GCTCC 2 cut(s) 114, 817
Lsp1109I GCAGC 2 cut(s) 1007, 1018
LweI GCATC 4 cut(s) 160, 402, 691, 839
MaeI CTAG 2 cut(s) 983, 1092
MaeIII GTNAC 1 cut(s) 127
MalI GATC 6 cut(s) 663, 712, 780, 921, 1026, 1185
MboI GATC 6 cut(s) 661, 710, 778, 919, 1024, 1183
MfeI CAATTG 1 cut(s) 741
MflI RGATCY 1 cut(s) 778
MhlI GDGCHC 1 cut(s) 913
MlsI TGGCCA 1 cut(s) 335
MluNI TGGCCA 1 cut(s) 335
MmeI TCCRAC 2 cut(s) 325, 583
Mox20I TGGCCA 1 cut(s) 335
Mph1103I ATGCAT 2 cut(s) 556, 880
MroXI GAANNNNTTC 1 cut(s) 250
MscI TGGCCA 1 cut(s) 335
MseI TTAA 1 cut(s) 597
MslI CAYNNNNRTG 1 cut(s) 426
Msp20I TGGCCA 1 cut(s) 335
MspR9I CCNGG 2 cut(s) 654, 835
MunI CAATTG 1 cut(s) 741
Mva1269I GAATGC 1 cut(s) 554
MvaI CCWGG 2 cut(s) 654, 835
MwoI GCNNNNNNNGC 1 cut(s) 1204
NdeI CATATG 1 cut(s) 852
NdeII GATC 6 cut(s) 661, 710, 778, 919, 1024, 1183
NlaIII CATG 8 cut(s) 69, 159, 482, 493, 689, 789, 878, 882
NlaIV GGNNCC 5 cut(s) 116, 291, 292, 813, 1065
NmuCI GTSAC 1 cut(s) 127
NsiI ATGCAT 2 cut(s) 556, 880
NspI RCATGY 1 cut(s) 482
PagI TCATGA 1 cut(s) 685
PciI ACATGT 1 cut(s) 478
PctI GAATGC 1 cut(s) 554
PdmI GAANNNNTTC 1 cut(s) 250
PfeI GAWTC 6 cut(s) 348, 493, 522, 585, 856, 947
PflMI CCANNNNNTGG 1 cut(s) 507
PkrI GCNGC 2 cut(s) 997, 1033
PpuMI RGGWCCY 1 cut(s) 290
PscI ACATGT 1 cut(s) 478
Psp5II RGGWCCY 1 cut(s) 290
Psp6I CCWGG 2 cut(s) 652, 833
PspFI CCCAGC 1 cut(s) 1176
PspGI CCWGG 2 cut(s) 652, 833
PspN4I GGNNCC 5 cut(s) 116, 291, 292, 813, 1065
PspPI GGNCC 3 cut(s) 34, 290, 471
PspPPI RGGWCCY 1 cut(s) 290
PstI CTGCAG 2 cut(s) 100, 997
PsuI RGATCY 1 cut(s) 778
RsaI GTAC 2 cut(s) 720, 956
RsaNI GTAC 2 cut(s) 719, 955
RseI CAYNNNNRTG 1 cut(s) 426
SaqAI TTAA 1 cut(s) 597
SatI GCNGC 2 cut(s) 996, 1032
Sau3AI GATC 6 cut(s) 661, 710, 778, 919, 1024, 1183
Sau96I GGNCC 3 cut(s) 34, 290, 471
ScrFI CCNGG 2 cut(s) 654, 835
SduI GDGCHC 1 cut(s) 913
SfaNI GCATC 4 cut(s) 160, 402, 691, 839
SfcI CTRYAG 2 cut(s) 96, 993
SinI GGWCC 2 cut(s) 34, 290
SmiMI CAYNNNNRTG 1 cut(s) 426
SspMI CTAG 2 cut(s) 983, 1092
StyD4I CCNGG 2 cut(s) 652, 833
StyI CCWWGG 1 cut(s) 580
TaaI ACNGT 1 cut(s) 971
TaqI TCGA 3 cut(s) 588, 922, 1128
TatI WGTACW 2 cut(s) 718, 954
TfiI GAWTC 6 cut(s) 348, 493, 522, 585, 856, 947
Tru1I TTAA 1 cut(s) 597
Tru9I TTAA 1 cut(s) 597
TscAI CASTG 1 cut(s) 845
TseFI GTSAC 1 cut(s) 127
TseI GCWGC 2 cut(s) 995, 1031
Tsp45I GTSAC 1 cut(s) 127
TspDTI ATGAA 9 cut(s) 17, 54, 410, 506, 547, 588, 780, 863, 953
TspRI CASTG 1 cut(s) 845
Van91I CCANNNNNTGG 1 cut(s) 507
VneI GTGCAC 1 cut(s) 909
VpaK11BI GGWCC 2 cut(s) 34, 290
XagI CCTNNNNNAGG 1 cut(s) 981
XapI RAATTY 2 cut(s) 642, 1131
XceI RCATGY 1 cut(s) 482
XmiI GTMKAC 1 cut(s) 1046
XmnI GAANNNNTTC 1 cut(s) 250
XspI CTAG 2 cut(s) 983, 1092
Zsp2I ATGCAT 2 cut(s) 556, 880
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.