Rorug07G0049500

Protein of unknown function (DUF1517)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000627
Physical Location & Seq
Reverse (-)
3556069 .. 3560593
4525 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0049500.1

Sequence Viewer

Length: 2181 bp
ATGGATGACGATGGAGATATTGATATGTTTTTCTCCAATTGGAACAACAAGAATCCAACTGATCGCCTCTTTGTTGTCTCTTGCTTTGTTGCTGCCCTAGTTGGAATTCTGACCATAGCCTATTCTGCCTTCCAATGGAGGAGAAACATCAATCTAAGTTGGATGAAAGCCATAGCCAGGTCAAAGAAAAATCCAAAAGCACGTCACAAAGTTCCCGTGGCTCCTCATACTTGGGTTCTAGAATCTGTATCTCGTGGAAAGAACTTTAATTGCTGTGTGTGTTTTAATACCACAGGCCCTTCTCAAACCCTCGGCCCTCTGGCAGCTTCAGACAGTTTTCTGCACCGTTGCAGTATTTGTGATGCAGCGGCTCATCTAAGTTGCTCCTCTACTGCACAAAAGGACTGCAAGTGTGTATCTATGATTGGTTATGAGCGTGTAATGCACCAATGGGCTGTCCGTTGGACAGAGGTAACGGACCAACCCGATGAGACTTCTTTCTGTAGCTACTGTGAAGAGCCCTGTAGTGGTTCCTTTCTTAGTGGATCCCCAATATGGTGCTGCTTGTGGTGTCAACGACTGGTACATGTTGATTGCCACAGTAGCATGTCCAATGAAACAGGTGATGTATGTGATTTAGGTCCGTTCAGAAGGTTGATTCTATCTCCCCTTCATGTTAAGGAGTTGAACCAGAATCCATCAGGTGGGTTTTTGAGCTCAATTACTCATGGAGCCAATGAGATAGCATCTACAGTACGTGCAAGTATTAGGAGTCAGAGCAAGAAGTACAAGCATGGAAATGCAATCTCTACTGGGACCGGAAATAGTGGTAGTACTGGTGATATGTCTACTGAAAGCACAGCTGATACCAATCAAGCTGTTAATGTTTCTCATGAAATGGAGGAAAATTGTAATGGTACCGTGGATGTGGATTTGCAGCACCAAGATGCTGATGTAGATAACAAATTGGATTCTAAGCCCAGTTTCCAAAAGAGCTCGTCTATGACTAAGAAGGATGAGTCTTTACGGATGAAACAAAGATATGAGTTGACTGATTTATCGTCAGATGCAAGACCCTTGTTGGTTTTCATCAACAAGAAGAGTGGAGCTCAGCGTGGGAATTCACTGAGGCAACGTTTGAATATTCTTCTCAACCCCGTCCAGGTATTTGAACTGAGTTCAGCACAGGGACCAGAGGTGGGTCTGTACTTATTCAGAAAGGTGCCACACTTCAGAGTTCTTGTTTGCGGGGGAGATGGTACTGTTGGTTGGGTTCTGGATGCCATTGAGAAGCAAAATTTCGTCTCTCCTCCTCCAGTTGCTATTCTTCCTGCCGGAACGGGGAATGATCTGGCTAGAGTTTTATCGTGGGGAGGTGGTTTGGGTGCAGTGGAGAGACAAGGAGGCCTTTGCACTGTTCTGCACCACATAGAGCATGCCGCTGTAACCATTCTTGACCGGTGGAAGGTGGCAATTTTAAATCAACAGGGAAAGCAACTTCAACCGCCAAAATTTATGAACAATTATCTTGGAATTGGATGTGATGCCAAGGTTGCTCTGGACATTCATAATCTTCGTGAGGAGAACCCGGAGAAGTTTTATAATCAGTTCATGAATAAAGTTCTTTATGCAAGAGAAGGGGCCAAGGGTATAATGGATAGAACATTTGTAGATTTTCCTTGGCAAGTTCGAGTTGAAGTAGATGGTGTTGAGATAGAGGTCCCTGAGGATGCAGAAGGTGTACTTGTTGCTAATATTGGAAGCTACATGGGTGGCGTAGATTTGTGGCAAAATGAAGATGAAACATATGATAATTTTGATCCGCAATCCATGCATGACAAAATACTTGAGGTTGTGAGCATATCTGGAACATGGCACCTTGGGAAACTACAGGTTGGGCTTTCTCGTGCTCGAAGACTTGCGCAGGGCCAGTCAATTAAGATACAGCTTTGTGCGGCATTTCCTGTTCAGATTGATGGGGAGCCTTGGTTTCAGCAACCTTGTACATTGTCTATATCCCATCATGGCCAGGCATTCATGTTGAAGAGGGCTGCTGAGGAGCCACTTGGTCATGCAGCTGCCATTATCACCGATGTCCTTGAGAATGCTGAAACGACCCATGTAATCAACGCTTCACAGAAGCGAGCTCTTCTTCAAGAAATGGCACTCAGACTGACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1517

Protein Analysis

726

Amino Acids

80.24

Weight (kDa)

6.16

Isoelectric Point (pI)

46.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C1_1 PF00130 149 - 208 5e-09 Phorbol esters/diacylglycerol binding domain (C1 domain)
DAGK_cat PF00781 359 - 459 1.7e-25 Diacylglycerol kinase catalytic domain
DAGK_acc PF00609 505 - 662 9.8e-57 Diacylglycerol kinase accessory domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1602
Acc16I TGCGCA 1 cut(s) 1923
Acc65I GGTACC 1 cut(s) 917
AccB1I GGYRCC 3 cut(s) 917, 1222, 1875
AccB7I CCANNNNNTGG 1 cut(s) 704
AccI GTMKAC 1 cut(s) 848
AciI CCGC 6 cut(s) 368, 1248, 1440, 1505, 1823, 1955
AclI AACGTT 1 cut(s) 1135
AclWI GGATC 3 cut(s) 540, 553, 1814
AcoI YGGCCR 1 cut(s) 2026
AcsI RAATTY 4 cut(s) 105, 1120, 1297, 1511
AcuI CTGAAG 2 cut(s) 312, 1216
AfaI GTAC 9 cut(s) 585, 756, 788, 835, 919, 1208, 1261, 1743, 2005
AfiI CCNNNNNNNGG 9 cut(s) 135, 177, 527, 555, 704, 1162, 1199, 1341, 1465
AflIII ACRYGT 1 cut(s) 586
AgeI ACCGGT 1 cut(s) 1458
AgsI TTSAA 7 cut(s) 688, 1141, 1172, 1502, 1697, 2044, 2156
AjiI CACGTC 1 cut(s) 203
AjnI CCWGG 3 cut(s) 176, 1161, 2028
Alw21I GWGCWC 5 cut(s) 719, 998, 1111, 1912, 2149
Alw26I GTCTC 4 cut(s) 82, 485, 1309, 1390
AlwI GGATC 3 cut(s) 540, 553, 1814
AoxI GGCC 6 cut(s) 295, 313, 1405, 1641, 1927, 2026
ApeKI GCWGC 8 cut(s) 92, 323, 365, 561, 937, 2051, 2075, 2078
ApoI RAATTY 4 cut(s) 105, 1120, 1297, 1511
AsiGI ACCGGT 1 cut(s) 1458
Asp718I GGTACC 1 cut(s) 917
AspLEI GCGC 1 cut(s) 1924
AspS9I GGNCC 9 cut(s) 296, 314, 478, 641, 816, 1190, 1641, 1720, 1927
AsuC2I CCSGG 1 cut(s) 1589
AsuHPI GGTGA 3 cut(s) 635, 851, 2080
AvaII GGWCC 5 cut(s) 478, 641, 816, 1190, 1720
AxyI CCTNAGG 1 cut(s) 1725
BalI TGGCCA 1 cut(s) 2028
BamHI GGATCC 1 cut(s) 545
BanI GGYRCC 3 cut(s) 917, 1222, 1875
BanII GRGCYC 5 cut(s) 522, 719, 998, 1111, 2149
BauI CACGAG 2 cut(s) 252, 1905
BbsI GAAGAC 1 cut(s) 1921
Bbv12I GWGCWC 5 cut(s) 719, 998, 1111, 1912, 2149
BbvCI CCTCAGC 1 cut(s) 2055
BbvI GCAGC 8 cut(s) 79, 335, 377, 548, 949, 2038, 2065, 2087
BccI CCATC 6 cut(s) 5, 706, 1250, 1697, 1970, 2028
BciT130I CCWGG 3 cut(s) 178, 1163, 2030
BcnI CCSGG 1 cut(s) 1589
BcoDI GTCTC 4 cut(s) 82, 485, 1309, 1390
BfaI CTAG 3 cut(s) 98, 239, 1356
BfmI CTRYAG 4 cut(s) 502, 523, 750, 1889
BlpI GCTNAGC 1 cut(s) 1110
BmcAI AGTACT 1 cut(s) 835
Bme1390I CCNGG 4 cut(s) 178, 1163, 1589, 2030
Bme18I GGWCC 5 cut(s) 478, 641, 816, 1190, 1720
BmgBI CACGTC 1 cut(s) 203
BmgT120I GGNCC 9 cut(s) 296, 314, 478, 641, 816, 1190, 1641, 1720, 1927
BmrFI CCNGG 4 cut(s) 178, 1163, 1589, 2030
BmrI ACTGGG 2 cut(s) 822, 975
BmsI GCATC 7 cut(s) 352, 755, 937, 1057, 1270, 1534, 1720
BmuI ACTGGG 2 cut(s) 822, 975
BpiI GAAGAC 1 cut(s) 1921
BplI GAGNNNNNCTC 2 cut(s) 1093, 1125
BpmI CTGGAG 1 cut(s) 1299
Bpu10I CCTNAGC 1 cut(s) 2055
Bpu1102I GCTNAGC 1 cut(s) 1110
BpuEI CTTGAG 2 cut(s) 1868, 2120
BpuMI CCSGG 1 cut(s) 1589
BsaAI YACGTR 1 cut(s) 758
BsaBI GATNNNNATC 1 cut(s) 870
BsaJI CCNNGG 8 cut(s) 216, 310, 921, 1548, 1644, 1679, 1879, 1985
BsaWI WCCGGW 2 cut(s) 818, 1458
BsaXI ACNNNNNCTCC 2 cut(s) 1973, 2003
Bsc4I CCNNNNNNNGG 9 cut(s) 135, 177, 527, 555, 704, 1162, 1199, 1341, 1465
Bse118I RCCGGY 1 cut(s) 1458
Bse1I ACTGG 6 cut(s) 585, 817, 841, 981, 1316, 1930
Bse21I CCTNAGG 1 cut(s) 1725
Bse8I GATNNNNATC 1 cut(s) 870
BseBI CCWGG 3 cut(s) 178, 1163, 2030
BseDI CCNNGG 8 cut(s) 216, 310, 921, 1548, 1644, 1679, 1879, 1985
BseGI GGATG 8 cut(s) 10, 168, 931, 1021, 1035, 1285, 1544, 1735
BseJI GATNNNNATC 1 cut(s) 870
BseLI CCNNNNNNNGG 9 cut(s) 135, 177, 527, 555, 704, 1162, 1199, 1341, 1465
BseMII CTCAG 5 cut(s) 1118, 1124, 1166, 1716, 2046
BseNI ACTGG 6 cut(s) 585, 817, 841, 981, 1316, 1930
BseRI GAGGAG 7 cut(s) 154, 213, 376, 1299, 1302, 1595, 2072
BseXI GCAGC 8 cut(s) 79, 335, 377, 548, 949, 2038, 2065, 2087
BsgI GTGCAG 4 cut(s) 326, 378, 1406, 1407
BshFI GGCC 6 cut(s) 297, 315, 1407, 1643, 1929, 2028
BshNI GGYRCC 3 cut(s) 917, 1222, 1875
BshTI ACCGGT 1 cut(s) 1458
BsiHKAI GWGCWC 5 cut(s) 719, 998, 1111, 1912, 2149
BsiSI CCGG 4 cut(s) 819, 1335, 1459, 1589
BslFI GGGAC 3 cut(s) 829, 1203, 1706
BslI CCNNNNNNNGG 9 cut(s) 135, 177, 527, 555, 704, 1162, 1199, 1341, 1465
BsmAI GTCTC 4 cut(s) 82, 485, 1309, 1390
BsmBI CGTCTC 1 cut(s) 1309
BsmFI GGGAC 3 cut(s) 829, 1203, 1706
BsmI GAATGC 2 cut(s) 2033, 2110
BsnI GGCC 6 cut(s) 297, 315, 1407, 1643, 1929, 2028
Bsp1286I GDGCHC 6 cut(s) 522, 719, 998, 1111, 1912, 2149
Bsp1407I TGTACA 1 cut(s) 2003
Bsp143I GATC 4 cut(s) 61, 545, 1348, 1819
Bsp1720I GCTNAGC 1 cut(s) 1110
BspACI CCGC 6 cut(s) 368, 1248, 1440, 1505, 1823, 1955
BspANI GGCC 6 cut(s) 297, 315, 1407, 1643, 1929, 2028
BspCNI CTCAG 6 cut(s) 1119, 1123, 1167, 1717, 2047, 2181
BspHI TCATGA 2 cut(s) 892, 1611
BspPI GGATC 3 cut(s) 540, 553, 1814
BspQI GCTCTTC 2 cut(s) 510, 2154
BspT107I GGYRCC 3 cut(s) 917, 1222, 1875
BsrFI RCCGGY 1 cut(s) 1458
BsrGI TGTACA 1 cut(s) 2003
BsrI ACTGG 6 cut(s) 585, 817, 841, 981, 1316, 1930
BssAI RCCGGY 1 cut(s) 1458
BssECI CCNNGG 8 cut(s) 216, 310, 921, 1548, 1644, 1679, 1879, 1985
BssMI GATC 4 cut(s) 61, 545, 1348, 1819
BssSI CACGAG 2 cut(s) 252, 1905
BssT1I CCWWGG 5 cut(s) 1548, 1644, 1679, 1879, 1985
Bst2BI CACGAG 2 cut(s) 252, 1905
Bst2UI CCWGG 3 cut(s) 178, 1163, 2030
Bst4CI ACNGT 8 cut(s) 335, 347, 512, 602, 754, 922, 1264, 1417
Bst6I CTCTTC 4 cut(s) 510, 1094, 2039, 2154
BstAPI GCANNNNNTGC 1 cut(s) 1831
BstAUI TGTACA 1 cut(s) 2003
BstBAI YACGTR 1 cut(s) 758
BstC8I GCNNGC 2 cut(s) 1437, 2145
BstDSI CCRYGG 2 cut(s) 216, 921
BstF5I GGATG 8 cut(s) 10, 168, 931, 1021, 1035, 1285, 1544, 1735
BstHHI GCGC 1 cut(s) 1924
BstKTI GATC 4 cut(s) 64, 548, 1351, 1822
BstMAI GTCTC 4 cut(s) 82, 485, 1309, 1390
BstMBI GATC 4 cut(s) 61, 545, 1348, 1819
BstMWI GCNNNNNNNGC 5 cut(s) 125, 442, 603, 1553, 1831
BstNI CCWGG 3 cut(s) 178, 1163, 2030
BstNSI RCATGY 3 cut(s) 590, 610, 1439
BstSCI CCNGG 4 cut(s) 176, 1161, 1587, 2028
BstSFI CTRYAG 4 cut(s) 502, 523, 750, 1889
BstV1I GCAGC 8 cut(s) 79, 335, 377, 548, 949, 2038, 2065, 2087
BstV2I GAAGAC 1 cut(s) 1921
BstX2I RGATCY 1 cut(s) 545
BstYI RGATCY 1 cut(s) 545
Bsu36I CCTNAGG 1 cut(s) 1725
BsuRI GGCC 6 cut(s) 297, 315, 1407, 1643, 1929, 2028
BtgI CCRYGG 2 cut(s) 216, 921
BtrI CACGTC 1 cut(s) 203
BtsCI GGATG 8 cut(s) 10, 168, 931, 1021, 1035, 1285, 1544, 1735
BtsI GCAGTG 1 cut(s) 1395
BtsIMutI CAGTG 3 cut(s) 1124, 1395, 1413
Cac8I GCNNGC 2 cut(s) 1437, 2145
CciI TCATGA 2 cut(s) 892, 1611
CfoI GCGC 1 cut(s) 1924
Cfr10I RCCGGY 1 cut(s) 1458
Cfr13I GGNCC 9 cut(s) 296, 314, 478, 641, 816, 1190, 1641, 1720, 1927
Csp6I GTAC 9 cut(s) 584, 755, 787, 834, 918, 1207, 1260, 1742, 2004
CspAI ACCGGT 1 cut(s) 1458
CspCI CAANNNNNGTGG 2 cut(s) 1084, 1119
CviQI GTAC 9 cut(s) 584, 755, 787, 834, 918, 1207, 1260, 1742, 2004
DpnI GATC 4 cut(s) 63, 547, 1350, 1821
DpnII GATC 4 cut(s) 61, 545, 1348, 1819
DraI TTTAAA 1 cut(s) 1479
EaeI YGGCCR 1 cut(s) 2026
Eam1104I CTCTTC 4 cut(s) 510, 1094, 2039, 2154
EarI CTCTTC 4 cut(s) 510, 1094, 2039, 2154
Ecl136II GAGCTC 4 cut(s) 717, 996, 1109, 2147
Eco130I CCWWGG 5 cut(s) 1548, 1644, 1679, 1879, 1985
Eco147I AGGCCT 1 cut(s) 1407
Eco24I GRGCYC 5 cut(s) 522, 719, 998, 1111, 2149
Eco47I GGWCC 5 cut(s) 478, 641, 816, 1190, 1720
Eco53kI GAGCTC 4 cut(s) 717, 996, 1109, 2147
Eco57I CTGAAG 2 cut(s) 312, 1216
Eco81I CCTNAGG 1 cut(s) 1725
EcoICRI GAGCTC 4 cut(s) 717, 996, 1109, 2147
EcoO109I RGGNCCY 2 cut(s) 296, 1720
EcoRI GAATTC 2 cut(s) 105, 1120
EcoRII CCWGG 3 cut(s) 176, 1161, 2028
EcoT14I CCWWGG 5 cut(s) 1548, 1644, 1679, 1879, 1985
EcoT22I ATGCAT 1 cut(s) 1836
EcoT38I GRGCYC 5 cut(s) 522, 719, 998, 1111, 2149
ErhI CCWWGG 5 cut(s) 1548, 1644, 1679, 1879, 1985
Esp3I CGTCTC 1 cut(s) 1309
FalI AAGNNNNNCTT 4 cut(s) 1392, 1424, 1728, 1760
FaqI GGGAC 3 cut(s) 829, 1203, 1706
FauI CCCGC 1 cut(s) 1241
FauNDI CATATG 1 cut(s) 1807
FblI GTMKAC 1 cut(s) 848
FokI GGATG 8 cut(s) 17, 175, 938, 1028, 1042, 1292, 1551, 1742
FriOI GRGCYC 5 cut(s) 522, 719, 998, 1111, 2149
FspBI CTAG 3 cut(s) 98, 239, 1356
FspI TGCGCA 1 cut(s) 1923
GlaI GCGC 1 cut(s) 1923
GsuI CTGGAG 1 cut(s) 1299
HaeIII GGCC 6 cut(s) 297, 315, 1407, 1643, 1929, 2028
HapII CCGG 4 cut(s) 819, 1335, 1459, 1589
HhaI GCGC 1 cut(s) 1924
Hin6I GCGC 1 cut(s) 1922
HinP1I GCGC 1 cut(s) 1922
HincII GTYRAC 2 cut(s) 575, 1050
HindII GTYRAC 2 cut(s) 575, 1050
HinfI GANTC 7 cut(s) 52, 242, 658, 694, 772, 971, 1019
HpaII CCGG 4 cut(s) 819, 1335, 1459, 1589
HphI GGTGA 3 cut(s) 635, 851, 2080
Hpy166II GTNNAC 4 cut(s) 575, 849, 1050, 1742
Hpy188I TCNGA 9 cut(s) 111, 331, 650, 777, 1066, 1217, 1235, 1971, 2171
Hpy188III TCNNGA 9 cut(s) 239, 893, 1277, 1454, 1559, 1577, 1612, 1866, 2156
Hpy8I GTNNAC 4 cut(s) 575, 849, 1050, 1742
HpyAV CCTTC 8 cut(s) 139, 309, 645, 680, 1006, 1459, 1631, 1730
HpyCH4III ACNGT 8 cut(s) 335, 347, 512, 602, 754, 922, 1264, 1417
HpyCH4IV ACGT 3 cut(s) 202, 757, 1135
HpyF10VI GCNNNNNNNGC 5 cut(s) 125, 442, 603, 1553, 1831
HpySE526I ACGT 3 cut(s) 202, 757, 1135
HspAI GCGC 1 cut(s) 1922
KpnI GGTACC 1 cut(s) 921
Kzo9I GATC 4 cut(s) 61, 545, 1348, 1819
LguI GCTCTTC 2 cut(s) 510, 2154
LmnI GCTCC 6 cut(s) 226, 389, 731, 1106, 1981, 2059
Lsp1109I GCAGC 8 cut(s) 79, 335, 377, 548, 949, 2038, 2065, 2087
LweI GCATC 7 cut(s) 352, 755, 937, 1057, 1270, 1534, 1720
MaeI CTAG 3 cut(s) 98, 239, 1356
MaeII ACGT 3 cut(s) 202, 757, 1135
MaeIII GTNAC 3 cut(s) 203, 472, 1444
MalI GATC 4 cut(s) 63, 547, 1350, 1821
MboI GATC 4 cut(s) 61, 545, 1348, 1819
MfeI CAATTG 1 cut(s) 37
MflI RGATCY 1 cut(s) 545
MhlI GDGCHC 6 cut(s) 522, 719, 998, 1111, 1912, 2149
MlsI TGGCCA 1 cut(s) 2028
MluNI TGGCCA 1 cut(s) 2028
MlyI GAGTC 2 cut(s) 781, 1028
MmeI TCCRAC 4 cut(s) 80, 82, 140, 443
Mox20I TGGCCA 1 cut(s) 2028
Mph1103I ATGCAT 1 cut(s) 1836
MscI TGGCCA 1 cut(s) 2028
MseI TTAA 6 cut(s) 267, 285, 678, 882, 1478, 1938
MslI CAYNNNNRTG 2 cut(s) 798, 945
Msp20I TGGCCA 1 cut(s) 2028
MspA1I CMGCKG 4 cut(s) 368, 863, 1442, 2078
MspI CCGG 4 cut(s) 819, 1335, 1459, 1589
MspR9I CCNGG 4 cut(s) 178, 1163, 1589, 2030
MunI CAATTG 1 cut(s) 37
Mva1269I GAATGC 2 cut(s) 2033, 2110
MvaI CCWGG 3 cut(s) 178, 1163, 2030
MwoI GCNNNNNNNGC 5 cut(s) 125, 442, 603, 1553, 1831
NciI CCSGG 1 cut(s) 1589
NdeI CATATG 1 cut(s) 1807
NdeII GATC 4 cut(s) 61, 545, 1348, 1819
NmeAIII GCCGAG 1 cut(s) 291
NmuCI GTSAC 1 cut(s) 203
NsbI TGCGCA 1 cut(s) 1923
NsiI ATGCAT 1 cut(s) 1836
NspI RCATGY 3 cut(s) 590, 610, 1439
PaeI GCATGC 1 cut(s) 1439
PagI TCATGA 2 cut(s) 892, 1611
PceI AGGCCT 1 cut(s) 1407
PciI ACATGT 1 cut(s) 586
PciSI GCTCTTC 2 cut(s) 510, 2154
PctI GAATGC 2 cut(s) 2033, 2110
PfeI GAWTC 5 cut(s) 52, 242, 658, 694, 971
PflMI CCANNNNNTGG 1 cut(s) 704
PinAI ACCGGT 1 cut(s) 1458
PleI GAGTC 2 cut(s) 780, 1027
PpsI GAGTC 2 cut(s) 780, 1027
Ppu21I YACGTR 1 cut(s) 758
PpuMI RGGWCCY 1 cut(s) 1720
PscI ACATGT 1 cut(s) 586
PsiI TTATAA 1 cut(s) 1602
Psp124BI GAGCTC 4 cut(s) 719, 998, 1111, 2149
Psp1406I AACGTT 1 cut(s) 1135
Psp5II RGGWCCY 1 cut(s) 1720
Psp6I CCWGG 3 cut(s) 176, 1161, 2028
PspGI CCWGG 3 cut(s) 176, 1161, 2028
PspPI GGNCC 9 cut(s) 296, 314, 478, 641, 816, 1190, 1641, 1720, 1927
PspPPI RGGWCCY 1 cut(s) 1720
PsuI RGATCY 1 cut(s) 545
PvuII CAGCTG 2 cut(s) 863, 2078
RsaI GTAC 9 cut(s) 585, 756, 788, 835, 919, 1208, 1261, 1743, 2005
RsaNI GTAC 9 cut(s) 584, 755, 787, 834, 918, 1207, 1260, 1742, 2004
RseI CAYNNNNRTG 2 cut(s) 798, 945
SacI GAGCTC 4 cut(s) 719, 998, 1111, 2149
SapI GCTCTTC 2 cut(s) 510, 2154
SaqAI TTAA 6 cut(s) 267, 285, 678, 882, 1478, 1938
Sau3AI GATC 4 cut(s) 61, 545, 1348, 1819
Sau96I GGNCC 9 cut(s) 296, 314, 478, 641, 816, 1190, 1641, 1720, 1927
ScaI AGTACT 1 cut(s) 835
SchI GAGTC 2 cut(s) 781, 1028
ScrFI CCNGG 4 cut(s) 178, 1163, 1589, 2030
SduI GDGCHC 6 cut(s) 522, 719, 998, 1111, 1912, 2149
SfaNI GCATC 7 cut(s) 352, 755, 937, 1057, 1270, 1534, 1720
SfcI CTRYAG 4 cut(s) 502, 523, 750, 1889
SinI GGWCC 5 cut(s) 478, 641, 816, 1190, 1720
SmiMI CAYNNNNRTG 2 cut(s) 798, 945
SmlI CTYRAG 2 cut(s) 1847, 2099
SmoI CTYRAG 2 cut(s) 1847, 2099
SphI GCATGC 1 cut(s) 1439
SseBI AGGCCT 1 cut(s) 1407
SsiI CCGC 6 cut(s) 368, 1248, 1440, 1505, 1823, 1955
SspI AATATT 2 cut(s) 1144, 1756
SspMI CTAG 3 cut(s) 98, 239, 1356
SstI GAGCTC 4 cut(s) 719, 998, 1111, 2149
StuI AGGCCT 1 cut(s) 1407
StyD4I CCNGG 4 cut(s) 176, 1161, 1587, 2028
StyI CCWWGG 5 cut(s) 1548, 1644, 1679, 1879, 1985
TaaI ACNGT 8 cut(s) 335, 347, 512, 602, 754, 922, 1264, 1417
TaiI ACGT 3 cut(s) 205, 760, 1138
TaqI TCGA 2 cut(s) 1690, 1912
TatI WGTACW 5 cut(s) 786, 833, 1206, 1741, 2003
TauI GCSGC 3 cut(s) 371, 1442, 1958
TfiI GAWTC 5 cut(s) 52, 242, 658, 694, 971
Tru1I TTAA 6 cut(s) 267, 285, 678, 882, 1478, 1938
Tru9I TTAA 6 cut(s) 267, 285, 678, 882, 1478, 1938
TscAI CASTG 3 cut(s) 1131, 1395, 1420
TseFI GTSAC 1 cut(s) 203
TseI GCWGC 8 cut(s) 92, 323, 365, 561, 937, 2051, 2075, 2078
Tsp45I GTSAC 1 cut(s) 203
TspGWI ACGGA 4 cut(s) 449, 491, 633, 1042
TspRI CASTG 3 cut(s) 1131, 1395, 1420
Van91I CCANNNNNTGG 1 cut(s) 704
VpaK11BI GGWCC 5 cut(s) 478, 641, 816, 1190, 1720
XapI RAATTY 4 cut(s) 105, 1120, 1297, 1511
XbaI TCTAGA 1 cut(s) 238
XceI RCATGY 3 cut(s) 590, 610, 1439
XcmI CCANNNNNNNNNTGG 2 cut(s) 1555, 1651
XmiI GTMKAC 1 cut(s) 848
XspI CTAG 3 cut(s) 98, 239, 1356
ZrmI AGTACT 1 cut(s) 835
Zsp2I ATGCAT 1 cut(s) 1836
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.