protein serine/threonine kinase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 173 |
| Acc36I | ACCTGC | 1 cut(s) | 43 |
| AclWI | GGATC | 4 cut(s) | 65, 78, 186, 199 |
| AcsI | RAATTY | 1 cut(s) | 206 |
| AcuI | CTGAAG | 1 cut(s) | 303 |
| AfaI | GTAC | 1 cut(s) | 60 |
| AluBI | AGCT | 2 cut(s) | 281, 312 |
| AluI | AGCT | 2 cut(s) | 281, 312 |
| AlwI | GGATC | 4 cut(s) | 65, 78, 186, 199 |
| ApeKI | GCWGC | 1 cut(s) | 119 |
| ApoI | RAATTY | 1 cut(s) | 206 |
| BamHI | GGATCC | 2 cut(s) | 70, 191 |
| BbvCI | CCTCAGC | 1 cut(s) | 227 |
| BbvI | GCAGC | 1 cut(s) | 131 |
| BfuAI | ACCTGC | 1 cut(s) | 43 |
| BisI | GCNGC | 1 cut(s) | 120 |
| BlsI | GCNGC | 1 cut(s) | 121 |
| BmiI | GGNNCC | 2 cut(s) | 72, 193 |
| BmsI | GCATC | 1 cut(s) | 43 |
| BpmI | CTGGAG | 1 cut(s) | 101 |
| Bpu10I | CCTNAGC | 1 cut(s) | 227 |
| Bse1I | ACTGG | 4 cut(s) | 15, 84, 187, 246 |
| BseMII | CTCAG | 3 cut(s) | 167, 241, 299 |
| BseNI | ACTGG | 4 cut(s) | 15, 84, 187, 246 |
| BseRI | GAGGAG | 1 cut(s) | 215 |
| BseXI | GCAGC | 1 cut(s) | 131 |
| BsgI | GTGCAG | 2 cut(s) | 60, 207 |
| Bsp1407I | TGTACA | 1 cut(s) | 58 |
| Bsp143I | GATC | 3 cut(s) | 63, 70, 191 |
| BspCNI | CTCAG | 3 cut(s) | 166, 240, 300 |
| BspLI | GGNNCC | 2 cut(s) | 72, 193 |
| BspMI | ACCTGC | 1 cut(s) | 43 |
| BspPI | GGATC | 4 cut(s) | 65, 78, 186, 199 |
| BsrGI | TGTACA | 1 cut(s) | 58 |
| BsrI | ACTGG | 4 cut(s) | 15, 84, 187, 246 |
| BssMI | GATC | 3 cut(s) | 63, 70, 191 |
| BstAUI | TGTACA | 1 cut(s) | 58 |
| BstDEI | CTNAG | 3 cut(s) | 153, 227, 308 |
| BstKTI | GATC | 3 cut(s) | 66, 73, 194 |
| BstMBI | GATC | 3 cut(s) | 63, 70, 191 |
| BstV1I | GCAGC | 1 cut(s) | 131 |
| BstX2I | RGATCY | 2 cut(s) | 70, 191 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 22, 241 |
| BstYI | RGATCY | 2 cut(s) | 70, 191 |
| BtsIMutI | CAGTG | 4 cut(s) | 22, 77, 180, 239 |
| BveI | ACCTGC | 1 cut(s) | 43 |
| Csp6I | GTAC | 1 cut(s) | 59 |
| CviAII | CATG | 1 cut(s) | 67 |
| CviJI | RGCY | 5 cut(s) | 26, 231, 281, 287, 312 |
| CviKI_1 | RGCY | 5 cut(s) | 26, 231, 281, 287, 312 |
| CviQI | GTAC | 1 cut(s) | 59 |
| DdeI | CTNAG | 3 cut(s) | 153, 227, 308 |
| DpnI | GATC | 3 cut(s) | 65, 72, 193 |
| DpnII | GATC | 3 cut(s) | 63, 70, 191 |
| Eco57I | CTGAAG | 1 cut(s) | 303 |
| FaeI | CATG | 1 cut(s) | 70 |
| FaiI | YATR | 7 cut(s) | 23, 68, 134, 164, 173, 218, 255 |
| FatI | CATG | 1 cut(s) | 66 |
| Fnu4HI | GCNGC | 1 cut(s) | 120 |
| Fsp4HI | GCNGC | 1 cut(s) | 120 |
| GluI | GCNGC | 1 cut(s) | 120 |
| GsuI | CTGGAG | 1 cut(s) | 101 |
| Hin1II | CATG | 1 cut(s) | 70 |
| HinfI | GANTC | 1 cut(s) | 151 |
| Hpy188I | TCNGA | 2 cut(s) | 39, 309 |
| HpyAV | CCTTC | 1 cut(s) | 94 |
| HpyCH4V | TGCA | 5 cut(s) | 77, 180, 188, 212, 257 |
| HpyF3I | CTNAG | 3 cut(s) | 153, 227, 308 |
| Hsp92II | CATG | 1 cut(s) | 70 |
| Kzo9I | GATC | 3 cut(s) | 63, 70, 191 |
| LmnI | GCTCC | 2 cut(s) | 49, 228 |
| LpnPI | CCDG | 9 cut(s) | 28, 38, 65, 87, 98, 150, 168, 174, 227 |
| Lsp1109I | GCAGC | 1 cut(s) | 131 |
| LweI | GCATC | 1 cut(s) | 43 |
| MalI | GATC | 3 cut(s) | 65, 72, 193 |
| MboI | GATC | 3 cut(s) | 63, 70, 191 |
| MboII | GAAGA | 2 cut(s) | 178, 276 |
| MfeI | CAATTG | 1 cut(s) | 123 |
| MflI | RGATCY | 2 cut(s) | 70, 191 |
| MluCI | AATT | 3 cut(s) | 123, 174, 206 |
| MlyI | GAGTC | 1 cut(s) | 145 |
| MnlI | CCTC | 4 cut(s) | 76, 97, 236, 247 |
| MseI | TTAA | 1 cut(s) | 276 |
| MslI | CAYNNNNRTG | 1 cut(s) | 113 |
| MunI | CAATTG | 1 cut(s) | 123 |
| NdeII | GATC | 3 cut(s) | 63, 70, 191 |
| NlaIII | CATG | 1 cut(s) | 70 |
| NlaIV | GGNNCC | 2 cut(s) | 72, 193 |
| PkrI | GCNGC | 1 cut(s) | 121 |
| PleI | GAGTC | 1 cut(s) | 145 |
| PpsI | GAGTC | 1 cut(s) | 145 |
| PsiI | TTATAA | 1 cut(s) | 173 |
| PspN4I | GGNNCC | 2 cut(s) | 72, 193 |
| PsuI | RGATCY | 2 cut(s) | 70, 191 |
| RsaI | GTAC | 1 cut(s) | 60 |
| RsaNI | GTAC | 1 cut(s) | 59 |
| RseI | CAYNNNNRTG | 1 cut(s) | 113 |
| SaqAI | TTAA | 1 cut(s) | 276 |
| SatI | GCNGC | 1 cut(s) | 120 |
| Sau3AI | GATC | 3 cut(s) | 63, 70, 191 |
| SchI | GAGTC | 1 cut(s) | 145 |
| SetI | ASST | 9 cut(s) | 8, 57, 108, 117, 139, 239, 253, 283, 314 |
| SfaNI | GCATC | 1 cut(s) | 43 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 113 |
| Sse9I | AATT | 3 cut(s) | 123, 174, 206 |
| TaqI | TCGA | 1 cut(s) | 95 |
| TasI | AATT | 3 cut(s) | 123, 174, 206 |
| TatI | WGTACW | 1 cut(s) | 58 |
| Tru1I | TTAA | 1 cut(s) | 276 |
| Tru9I | TTAA | 1 cut(s) | 276 |
| TscAI | CASTG | 4 cut(s) | 22, 84, 187, 246 |
| TseI | GCWGC | 1 cut(s) | 119 |
| TspDTI | ATGAA | 3 cut(s) | 113, 179, 312 |
| TspRI | CASTG | 4 cut(s) | 22, 84, 187, 246 |
| XapI | RAATTY | 1 cut(s) | 206 |