AT1G26665

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001018
Physical Location & Seq
Forward (+)
9214072 .. 9215557
1486 bp
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UTR
Exon/CDS
Intron
AT1G26665.2

Sequence Viewer

Length: 570 bp
ATGGATCCAACACAGAACACAAGTGCAGGGATTGGTGGGAGCAATGGTACAATCAGATATCAAACCAATGATGGGACTTCAACTGTGACCGTTGCTGATGATTCAAAGGAGAATCTTTCCCAAGTCATAAACTCTATTGAGAAAACTTTAGGAGTCCTTCATCAGTTACACCTTACCGTCACTTCTTTCACACCCGCGTCTCAACTCCATCTCCTCCAGCGCCTTAACTCTCTTGTGATGGAGCTAGATAACATGACTAAGTTGTCTGAGAAATGCAACATTCAAATCCCCATGGAGGTTCTTAACTTGATTGATGATGGGAAGAACCCGGATGAGTTTACGAAAGATGTTCTTAATAGCTGCATAGCTAGAAATCAAGTTACTAAGGGCAAGACTGATGCTTTTAAGGATTTGAGAAAACATATTCTGGAGGAGCTTGAACAGAATTTCCCTGATGAAGTCGATATGTATAGAGAAATCCGTGCTAGTTCTGCTGCTGAAGCGAAACGGTTGGCGCAATCGCAAAGTGTGTTACCAAATGGGGATGCAAAGGTCAAGAATGAGCTATAA

Protein Analysis

189

Amino Acids

20.91

Weight (kDa)

5.11

Isoelectric Point (pI)

28.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med10 PF09748 45 - 158 6.9e-34 Transcription factor subunit Med10 of Mediator complex
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 262
AccII CGCG 1 cut(s) 197
AciI CCGC 1 cut(s) 195
AclWI GGATC 1 cut(s) 12
AcsI RAATTY 1 cut(s) 445
AcuI CTGAAG 1 cut(s) 519
AfaI GTAC 1 cut(s) 49
AfiI CCNNNNNNNGG 2 cut(s) 72, 295
AgsI TTSAA 4 cut(s) 81, 105, 284, 440
AloI GAACNNNNNNTCC 2 cut(s) 432, 464
AluBI AGCT 5 cut(s) 244, 360, 368, 436, 565
AluI AGCT 5 cut(s) 244, 360, 368, 436, 565
Alw26I GTCTC 1 cut(s) 204
AlwI GGATC 1 cut(s) 12
ApeKI GCWGC 2 cut(s) 360, 494
ApoI RAATTY 1 cut(s) 445
AspLEI GCGC 2 cut(s) 222, 517
AsuC2I CCSGG 1 cut(s) 329
BamHI GGATCC 1 cut(s) 4
BbvI GCAGC 2 cut(s) 347, 481
BccI CCATC 4 cut(s) 65, 216, 232, 311
BcnI CCSGG 1 cut(s) 329
BcoDI GTCTC 1 cut(s) 204
BfaI CTAG 3 cut(s) 245, 369, 486
BfoI RGCGCY 1 cut(s) 223
BisI GCNGC 2 cut(s) 361, 495
BlsI GCNGC 2 cut(s) 362, 496
Bme1390I CCNGG 1 cut(s) 329
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 1 cut(s) 329
BmsI GCATC 2 cut(s) 388, 535
BpmI CTGGAG 2 cut(s) 200, 449
BpuMI CCSGG 1 cut(s) 329
BsaJI CCNNGG 1 cut(s) 291
BsaXI ACNNNNNCTCC 4 cut(s) 31, 61, 195, 225
Bsc4I CCNNNNNNNGG 2 cut(s) 72, 295
Bse3DI GCAATG 1 cut(s) 49
BseDI CCNNGG 1 cut(s) 291
BseGI GGATG 2 cut(s) 337, 550
BseLI CCNNNNNNNGG 2 cut(s) 72, 295
BseMI GCAATG 1 cut(s) 49
BseMII CTCAG 1 cut(s) 258
BseRI GAGGAG 2 cut(s) 203, 446
BseXI GCAGC 2 cut(s) 347, 481
BsgI GTGCAG 1 cut(s) 45
Bsh1236I CGCG 1 cut(s) 197
BsiSI CCGG 1 cut(s) 329
BslFI GGGAC 1 cut(s) 88
BslI CCNNNNNNNGG 2 cut(s) 72, 295
BsmAI GTCTC 1 cut(s) 204
BsmBI CGTCTC 1 cut(s) 204
BsmFI GGGAC 1 cut(s) 88
Bsp143I GATC 1 cut(s) 4
Bsp19I CCATGG 1 cut(s) 291
BspACI CCGC 1 cut(s) 195
BspCNI CTCAG 1 cut(s) 259
BspFNI CGCG 1 cut(s) 197
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 1 cut(s) 12
BsrDI GCAATG 1 cut(s) 49
BssECI CCNNGG 1 cut(s) 291
BssMI GATC 1 cut(s) 4
BssT1I CCWWGG 1 cut(s) 291
Bst4CI ACNGT 4 cut(s) 85, 91, 178, 510
BstDEI CTNAG 3 cut(s) 258, 267, 384
BstDSI CCRYGG 1 cut(s) 291
BstF5I GGATG 2 cut(s) 337, 550
BstFNI CGCG 1 cut(s) 197
BstH2I RGCGCY 1 cut(s) 223
BstHHI GCGC 2 cut(s) 222, 517
BstKTI GATC 1 cut(s) 7
BstMAI GTCTC 1 cut(s) 204
BstMBI GATC 1 cut(s) 4
BstMWI GCNNNNNNNGC 2 cut(s) 491, 500
BstSCI CCNGG 1 cut(s) 327
BstUI CGCG 1 cut(s) 197
BstV1I GCAGC 2 cut(s) 347, 481
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BtgI CCRYGG 1 cut(s) 291
BtsCI GGATG 2 cut(s) 337, 550
CfoI GCGC 2 cut(s) 222, 517
CseI GACGC 1 cut(s) 186
Csp6I GTAC 1 cut(s) 48
CviAII CATG 2 cut(s) 253, 292
CviJI RGCY 5 cut(s) 244, 360, 368, 436, 565
CviKI_1 RGCY 5 cut(s) 244, 360, 368, 436, 565
CviQI GTAC 1 cut(s) 48
DdeI CTNAG 3 cut(s) 258, 267, 384
DpnI GATC 1 cut(s) 6
DpnII GATC 1 cut(s) 4
DrdI GACNNNNNNGTC 1 cut(s) 262
DseDI GACNNNNNNGTC 1 cut(s) 262
Eco130I CCWWGG 1 cut(s) 291
Eco32I GATATC 1 cut(s) 59
Eco57I CTGAAG 1 cut(s) 519
EcoRV GATATC 1 cut(s) 59
EcoT14I CCWWGG 1 cut(s) 291
ErhI CCWWGG 1 cut(s) 291
Esp3I CGTCTC 1 cut(s) 204
FaeI CATG 2 cut(s) 256, 295
FaiI YATR 8 cut(s) 128, 254, 293, 365, 423, 467, 471, 568
FalI AAGNNNNNCTT 2 cut(s) 336, 368
FaqI GGGAC 1 cut(s) 88
FatI CATG 2 cut(s) 252, 291
FauI CCCGC 1 cut(s) 202
Fnu4HI GCNGC 2 cut(s) 361, 495
FokI GGATG 2 cut(s) 344, 557
Fsp4HI GCNGC 2 cut(s) 361, 495
FspBI CTAG 3 cut(s) 245, 369, 486
GlaI GCGC 2 cut(s) 221, 516
GluI GCNGC 2 cut(s) 361, 495
GsuI CTGGAG 2 cut(s) 200, 449
HaeII RGCGCY 1 cut(s) 223
HapII CCGG 1 cut(s) 329
HgaI GACGC 1 cut(s) 186
HhaI GCGC 2 cut(s) 222, 517
Hin1II CATG 2 cut(s) 256, 295
Hin6I GCGC 2 cut(s) 220, 515
HinP1I GCGC 2 cut(s) 220, 515
HinfI GANTC 3 cut(s) 101, 112, 153
HpaII CCGG 1 cut(s) 329
Hpy166II GTNNAC 1 cut(s) 339
Hpy188I TCNGA 2 cut(s) 56, 268
Hpy188III TCNNGA 2 cut(s) 428, 556
Hpy8I GTNNAC 1 cut(s) 339
HpyAV CCTTC 1 cut(s) 167
HpyCH4III ACNGT 4 cut(s) 85, 91, 178, 510
HpyCH4V TGCA 4 cut(s) 26, 276, 363, 548
HpyF10VI GCNNNNNNNGC 2 cut(s) 491, 500
HpyF3I CTNAG 3 cut(s) 258, 267, 384
Hsp92II CATG 2 cut(s) 256, 295
HspAI GCGC 2 cut(s) 220, 515
Kzo9I GATC 1 cut(s) 4
LmnI GCTCC 3 cut(s) 39, 241, 433
LpnPI CCDG 5 cut(s) 12, 230, 342, 413, 465
Lsp1109I GCAGC 2 cut(s) 347, 481
LweI GCATC 2 cut(s) 388, 535
MaeI CTAG 3 cut(s) 245, 369, 486
MaeIII GTNAC 5 cut(s) 85, 165, 178, 379, 531
MalI GATC 1 cut(s) 6
MboI GATC 1 cut(s) 4
MboII GAAGA 1 cut(s) 334
MflI RGATCY 1 cut(s) 4
MluCI AATT 1 cut(s) 445
MlyI GAGTC 1 cut(s) 162
MmeI TCCRAC 1 cut(s) 32
MnlI CCTC 3 cut(s) 224, 289, 424
MseI TTAA 4 cut(s) 225, 303, 354, 405
MspI CCGG 1 cut(s) 329
MspR9I CCNGG 1 cut(s) 329
MvnI CGCG 1 cut(s) 197
MwoI GCNNNNNNNGC 2 cut(s) 491, 500
NciI CCSGG 1 cut(s) 329
NcoI CCATGG 1 cut(s) 291
NdeII GATC 1 cut(s) 4
NlaIII CATG 2 cut(s) 256, 295
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 2 cut(s) 85, 178
PfeI GAWTC 2 cut(s) 101, 112
PkrI GCNGC 2 cut(s) 362, 496
PleI GAGTC 1 cut(s) 161
PpsI GAGTC 1 cut(s) 161
PspN4I GGNNCC 1 cut(s) 6
PsuI RGATCY 1 cut(s) 4
RsaI GTAC 1 cut(s) 49
RsaNI GTAC 1 cut(s) 48
SaqAI TTAA 4 cut(s) 225, 303, 354, 405
SatI GCNGC 2 cut(s) 361, 495
Sau3AI GATC 1 cut(s) 4
SchI GAGTC 1 cut(s) 162
ScrFI CCNGG 1 cut(s) 329
SetI ASST 8 cut(s) 174, 246, 300, 362, 370, 438, 555, 567
SfaNI GCATC 2 cut(s) 388, 535
Sse9I AATT 1 cut(s) 445
SsiI CCGC 1 cut(s) 195
SspMI CTAG 3 cut(s) 245, 369, 486
StyD4I CCNGG 1 cut(s) 327
StyI CCWWGG 1 cut(s) 291
TaaI ACNGT 4 cut(s) 85, 91, 178, 510
TaqI TCGA 1 cut(s) 462
TasI AATT 1 cut(s) 445
TfiI GAWTC 2 cut(s) 101, 112
Tru1I TTAA 4 cut(s) 225, 303, 354, 405
Tru9I TTAA 4 cut(s) 225, 303, 354, 405
TseFI GTSAC 2 cut(s) 85, 178
TseI GCWGC 2 cut(s) 360, 494
Tsp45I GTSAC 2 cut(s) 85, 178
TspDTI ATGAA 2 cut(s) 149, 471
TspGWI ACGGA 1 cut(s) 470
XapI RAATTY 1 cut(s) 445
XspI CTAG 3 cut(s) 245, 369, 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.