Rroxscaffold_3G00246910

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001018
Physical Location & Seq
Forward (+)
40214858 .. 40215415
558 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00246910.1

Sequence Viewer

Length: 558 bp
ATGAGAGGGCCTCCAGCTTTTGCAGAGCGACTCGGAAGAGCGAAACCTAGGTTTCGTGATGGCGGCGAGGGAGCGTCGTGGTTTCCAAACCAGACGACGCGAGTGAAGGGCAGCGGGGGCTTGGAGAGGCACCGGTCTCTTTTGCAAAGGCGTTGGGATCTCGATCAAGTTTTTCGAAGGCTTCGGCCGAGTCACCGCTCGACCGAGGGTGGTTTTATGAGCGTGGGTTGCCGGATTTGGTCGATGCCGAGGCGTTGTCGTCGACGTCGTCGTGGTTTTGGGGCAACTAGGATCCGCATCGTTGTGGCGGCCTCCACCGACCAAGGTTGGGCTCAAGTCGGGCCCACCGGAGACGATGGCTCCGGTGCGGTCGGGCTATGGGGCCGTGCCGCGCTTCGTGGAGGCCGCAGGAGGGCCGACGAGGACTTGGTCGGTGGCTTAGACCGGTCCGCCGGAGATCGGTGCGGCGGCGGGCCACGAGCAAGAAGACGGTCCGAAAAATCACCGGGAGACCCTAGTTTTGAATTAGGGTTGATCGGGCTGATTTCGGGCCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

185

Amino Acids

20.34

Weight (kDa)

11.81

Isoelectric Point (pI)

72.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 268
AccB1I GGYRCC 1 cut(s) 129
AccBSI CCGCTC 1 cut(s) 198
AccI GTMKAC 1 cut(s) 262
AccII CGCG 2 cut(s) 100, 392
AclWI GGATC 3 cut(s) 165, 286, 299
AcoI YGGCCR 1 cut(s) 185
AcyI GRCGYC 1 cut(s) 265
AfiI CCNNNNNNNGG 3 cut(s) 328, 412, 459
AgeI ACCGGT 2 cut(s) 132, 444
AgsI TTSAA 1 cut(s) 524
AluBI AGCT 1 cut(s) 17
AluI AGCT 1 cut(s) 17
Alw26I GTCTC 3 cut(s) 141, 345, 504
AlwI GGATC 3 cut(s) 165, 286, 299
AoxI GGCC 9 cut(s) 8, 185, 309, 341, 382, 403, 414, 473, 550
ApaI GGGCCC 1 cut(s) 345
ApeKI GCWGC 1 cut(s) 111
ArsI GACNNNNNNTTYG 2 cut(s) 503, 535
AsiGI ACCGGT 2 cut(s) 132, 444
AspA2I CCTAGG 1 cut(s) 47
AspLEI GCGC 1 cut(s) 394
AspS9I GGNCC 9 cut(s) 8, 341, 342, 382, 414, 447, 473, 492, 550
AsuC2I CCSGG 1 cut(s) 507
AsuHPI GGTGA 2 cut(s) 185, 495
AsuII TTCGAA 1 cut(s) 175
AvaII GGWCC 2 cut(s) 447, 492
AvrII CCTAGG 1 cut(s) 47
BaeGI GKGCMC 1 cut(s) 345
BamHI GGATCC 1 cut(s) 291
BanI GGYRCC 1 cut(s) 129
BanII GRGCYC 2 cut(s) 334, 345
BauI CACGAG 1 cut(s) 477
BbsI GAAGAC 1 cut(s) 493
BbvI GCAGC 1 cut(s) 123
BccI CCATC 2 cut(s) 53, 350
BceAI ACGGC 1 cut(s) 369
BcnI CCSGG 1 cut(s) 507
BcoDI GTCTC 3 cut(s) 141, 345, 504
BfaI CTAG 3 cut(s) 48, 288, 516
BisI GCNGC 7 cut(s) 64, 112, 309, 390, 406, 466, 469
BlnI CCTAGG 1 cut(s) 47
BlsI GCNGC 7 cut(s) 65, 113, 310, 391, 407, 467, 470
Bme1390I CCNGG 1 cut(s) 507
Bme18I GGWCC 2 cut(s) 447, 492
BmgT120I GGNCC 9 cut(s) 8, 341, 342, 382, 414, 447, 473, 492, 550
BmiI GGNNCC 5 cut(s) 131, 293, 343, 361, 383
BmrFI CCNGG 1 cut(s) 507
BmsI GCATC 2 cut(s) 234, 306
BpiI GAAGAC 1 cut(s) 493
BplI GAGNNNNNCTC 1 cut(s) 27
Bpu14I TTCGAA 1 cut(s) 175
BpuEI CTTGAG 1 cut(s) 318
BpuMI CCSGG 1 cut(s) 507
BsaBI GATNNNNATC 2 cut(s) 162, 296
BsaHI GRCGYC 1 cut(s) 265
BsaI GGTCTC 2 cut(s) 141, 504
BsaJI CCNNGG 4 cut(s) 47, 204, 248, 322
BsaWI WCCGGW 4 cut(s) 132, 347, 362, 444
BsaXI ACNNNNNCTCC 2 cut(s) 344, 374
Bsc4I CCNNNNNNNGG 3 cut(s) 328, 412, 459
Bse118I RCCGGY 2 cut(s) 132, 444
Bse8I GATNNNNATC 2 cut(s) 162, 296
BseDI CCNNGG 4 cut(s) 47, 204, 248, 322
BseJI GATNNNNATC 2 cut(s) 162, 296
BseLI CCNNNNNNNGG 3 cut(s) 328, 412, 459
BseSI GKGCMC 1 cut(s) 345
BseX3I CGGCCG 1 cut(s) 185
BseXI GCAGC 1 cut(s) 123
Bsh1236I CGCG 2 cut(s) 100, 392
Bsh1285I CGRYCG 3 cut(s) 188, 204, 372
BshFI GGCC 9 cut(s) 10, 187, 311, 343, 384, 405, 416, 475, 552
BshNI GGYRCC 1 cut(s) 129
BshTI ACCGGT 2 cut(s) 132, 444
BsiEI CGRYCG 3 cut(s) 188, 204, 372
BsiSI CCGG 7 cut(s) 133, 232, 348, 363, 445, 453, 506
BslI CCNNNNNNNGG 3 cut(s) 328, 412, 459
BsmAI GTCTC 3 cut(s) 141, 345, 504
BsmBI CGTCTC 1 cut(s) 345
BsnI GGCC 9 cut(s) 10, 187, 311, 343, 384, 405, 416, 475, 552
Bso31I GGTCTC 2 cut(s) 141, 504
Bsp119I TTCGAA 1 cut(s) 175
Bsp120I GGGCCC 1 cut(s) 341
Bsp1286I GDGCHC 2 cut(s) 334, 345
Bsp143I GATC 5 cut(s) 157, 163, 291, 457, 534
BspANI GGCC 9 cut(s) 10, 187, 311, 343, 384, 405, 416, 475, 552
BspFNI CGCG 2 cut(s) 100, 392
BspLI GGNNCC 5 cut(s) 131, 293, 343, 361, 383
BspPI GGATC 3 cut(s) 165, 286, 299
BspQI GCTCTTC 1 cut(s) 31
BspT104I TTCGAA 1 cut(s) 175
BspT107I GGYRCC 1 cut(s) 129
BspTNI GGTCTC 2 cut(s) 141, 504
BsrBI CCGCTC 1 cut(s) 198
BsrFI RCCGGY 2 cut(s) 132, 444
BssAI RCCGGY 2 cut(s) 132, 444
BssECI CCNNGG 4 cut(s) 47, 204, 248, 322
BssMI GATC 5 cut(s) 157, 163, 291, 457, 534
BssNI GRCGYC 1 cut(s) 265
BssSI CACGAG 1 cut(s) 477
BssT1I CCWWGG 2 cut(s) 47, 322
Bst2BI CACGAG 1 cut(s) 477
Bst4CI ACNGT 1 cut(s) 492
Bst6I CTCTTC 1 cut(s) 31
BstACI GRCGYC 1 cut(s) 265
BstBI TTCGAA 1 cut(s) 175
BstC8I GCNNGC 1 cut(s) 473
BstDEI CTNAG 1 cut(s) 439
BstFNI CGCG 2 cut(s) 100, 392
BstHHI GCGC 1 cut(s) 394
BstKTI GATC 5 cut(s) 160, 166, 294, 460, 537
BstMAI GTCTC 3 cut(s) 141, 345, 504
BstMBI GATC 5 cut(s) 157, 163, 291, 457, 534
BstMCI CGRYCG 3 cut(s) 188, 204, 372
BstMWI GCNNNNNNNGC 2 cut(s) 117, 228
BstSCI CCNGG 1 cut(s) 505
BstSLI GKGCMC 1 cut(s) 345
BstUI CGCG 2 cut(s) 100, 392
BstV1I GCAGC 1 cut(s) 123
BstV2I GAAGAC 1 cut(s) 493
BstX2I RGATCY 2 cut(s) 157, 291
BstYI RGATCY 2 cut(s) 157, 291
BstZI CGGCCG 1 cut(s) 185
BsuRI GGCC 9 cut(s) 10, 187, 311, 343, 384, 405, 416, 475, 552
Cac8I GCNNGC 1 cut(s) 473
CfoI GCGC 1 cut(s) 394
Cfr10I RCCGGY 2 cut(s) 132, 444
Cfr13I GGNCC 9 cut(s) 8, 341, 342, 382, 414, 447, 473, 492, 550
CpoI CGGWCCG 2 cut(s) 447, 492
CseI GACGC 2 cut(s) 63, 106
CspAI ACCGGT 2 cut(s) 132, 444
CspI CGGWCCG 2 cut(s) 447, 492
DdeI CTNAG 1 cut(s) 439
DpnI GATC 5 cut(s) 159, 165, 293, 459, 536
DpnII GATC 5 cut(s) 157, 163, 291, 457, 534
EaeI YGGCCR 1 cut(s) 185
EagI CGGCCG 1 cut(s) 185
Eam1104I CTCTTC 1 cut(s) 31
EarI CTCTTC 1 cut(s) 31
EciI GGCGGA 1 cut(s) 439
EclXI CGGCCG 1 cut(s) 185
Eco130I CCWWGG 2 cut(s) 47, 322
Eco24I GRGCYC 2 cut(s) 334, 345
Eco31I GGTCTC 2 cut(s) 141, 504
Eco47I GGWCC 2 cut(s) 447, 492
Eco52I CGGCCG 1 cut(s) 185
EcoO109I RGGNCCY 1 cut(s) 8
EcoT14I CCWWGG 2 cut(s) 47, 322
EcoT38I GRGCYC 2 cut(s) 334, 345
ErhI CCWWGG 2 cut(s) 47, 322
Esp3I CGTCTC 1 cut(s) 345
FaiI YATR 2 cut(s) 218, 379
FauI CCCGC 2 cut(s) 107, 464
FblI GTMKAC 1 cut(s) 262
Fnu4HI GCNGC 7 cut(s) 64, 112, 309, 390, 406, 466, 469
FriOI GRGCYC 2 cut(s) 334, 345
Fsp4HI GCNGC 7 cut(s) 64, 112, 309, 390, 406, 466, 469
FspBI CTAG 3 cut(s) 48, 288, 516
GlaI GCGC 1 cut(s) 393
GluI GCNGC 7 cut(s) 64, 112, 309, 390, 406, 466, 469
HaeIII GGCC 9 cut(s) 10, 187, 311, 343, 384, 405, 416, 475, 552
HapII CCGG 7 cut(s) 133, 232, 348, 363, 445, 453, 506
HgaI GACGC 2 cut(s) 63, 106
HhaI GCGC 1 cut(s) 394
Hin1I GRCGYC 1 cut(s) 265
Hin6I GCGC 1 cut(s) 392
HinP1I GCGC 1 cut(s) 392
HincII GTYRAC 1 cut(s) 263
HindII GTYRAC 1 cut(s) 263
HinfI GANTC 2 cut(s) 30, 190
HpaII CCGG 7 cut(s) 133, 232, 348, 363, 445, 453, 506
HphI GGTGA 2 cut(s) 185, 495
Hpy166II GTNNAC 1 cut(s) 263
Hpy188I TCNGA 2 cut(s) 35, 496
Hpy188III TCNNGA 2 cut(s) 56, 161
Hpy8I GTNNAC 1 cut(s) 263
Hpy99I CGWCG 7 cut(s) 79, 100, 264, 267, 270, 273, 422
HpyAV CCTTC 2 cut(s) 100, 171
HpyCH4III ACNGT 1 cut(s) 492
HpyCH4IV ACGT 1 cut(s) 265
HpyCH4V TGCA 2 cut(s) 23, 145
HpyF10VI GCNNNNNNNGC 2 cut(s) 117, 228
HpyF3I CTNAG 1 cut(s) 439
HpySE526I ACGT 1 cut(s) 265
Hsp92I GRCGYC 1 cut(s) 265
HspAI GCGC 1 cut(s) 392
Kzo9I GATC 5 cut(s) 157, 163, 291, 457, 534
LguI GCTCTTC 1 cut(s) 31
LmnI GCTCC 2 cut(s) 71, 365
Lsp1109I GCAGC 1 cut(s) 123
LweI GCATC 2 cut(s) 234, 306
MaeI CTAG 3 cut(s) 48, 288, 516
MaeII ACGT 1 cut(s) 265
MaeIII GTNAC 1 cut(s) 191
MalI GATC 5 cut(s) 159, 165, 293, 459, 536
MbiI CCGCTC 1 cut(s) 198
MboI GATC 5 cut(s) 157, 163, 291, 457, 534
MboII GAAGA 2 cut(s) 48, 498
MflI RGATCY 2 cut(s) 157, 291
MhlI GDGCHC 2 cut(s) 334, 345
MluCI AATT 1 cut(s) 524
MlyI GAGTC 2 cut(s) 24, 199
MnlI CCTC 9 cut(s) 21, 61, 120, 199, 243, 322, 395, 405, 415
MslI CAYNNNNRTG 1 cut(s) 302
MspA1I CMGCKG 1 cut(s) 114
MspI CCGG 7 cut(s) 133, 232, 348, 363, 445, 453, 506
MspR9I CCNGG 1 cut(s) 507
MvnI CGCG 2 cut(s) 100, 392
MwoI GCNNNNNNNGC 2 cut(s) 117, 228
NciI CCSGG 1 cut(s) 507
NdeII GATC 5 cut(s) 157, 163, 291, 457, 534
NlaIV GGNNCC 5 cut(s) 131, 293, 343, 361, 383
NmeAIII GCCGAG 2 cut(s) 213, 273
NmuCI GTSAC 1 cut(s) 191
NspV TTCGAA 1 cut(s) 175
PciSI GCTCTTC 1 cut(s) 31
PcsI WCGNNNNNNNCGW 2 cut(s) 265, 268
PflFI GACNNNGTC 2 cut(s) 267, 428
PinAI ACCGGT 2 cut(s) 132, 444
PkrI GCNGC 7 cut(s) 65, 113, 310, 391, 407, 467, 470
PleI GAGTC 2 cut(s) 24, 198
PpsI GAGTC 2 cut(s) 24, 198
PspN4I GGNNCC 5 cut(s) 131, 293, 343, 361, 383
PspOMI GGGCCC 1 cut(s) 341
PspPI GGNCC 9 cut(s) 8, 341, 342, 382, 414, 447, 473, 492, 550
PsuI RGATCY 2 cut(s) 157, 291
PsyI GACNNNGTC 2 cut(s) 267, 428
RseI CAYNNNNRTG 1 cut(s) 302
Rsr2I CGGWCCG 2 cut(s) 447, 492
RsrII CGGWCCG 2 cut(s) 447, 492
SalI GTCGAC 1 cut(s) 261
SapI GCTCTTC 1 cut(s) 31
SatI GCNGC 7 cut(s) 64, 112, 309, 390, 406, 466, 469
Sau3AI GATC 5 cut(s) 157, 163, 291, 457, 534
Sau96I GGNCC 9 cut(s) 8, 341, 342, 382, 414, 447, 473, 492, 550
SchI GAGTC 2 cut(s) 24, 199
ScrFI CCNGG 1 cut(s) 507
SduI GDGCHC 2 cut(s) 334, 345
SetI ASST 5 cut(s) 19, 49, 53, 268, 328
SfaNI GCATC 2 cut(s) 234, 306
SfuI TTCGAA 1 cut(s) 175
SgrDI CGTCGACG 1 cut(s) 261
SinI GGWCC 2 cut(s) 447, 492
SmiMI CAYNNNNRTG 1 cut(s) 302
SmlI CTYRAG 1 cut(s) 333
SmoI CTYRAG 1 cut(s) 333
Sse9I AATT 1 cut(s) 524
SspMI CTAG 3 cut(s) 48, 288, 516
StyD4I CCNGG 1 cut(s) 505
StyI CCWWGG 2 cut(s) 47, 322
TaaI ACNGT 1 cut(s) 492
TaiI ACGT 1 cut(s) 268
TaqI TCGA 5 cut(s) 162, 175, 200, 242, 262
TaqII GACCGA 1 cut(s) 218
TasI AATT 1 cut(s) 524
TauI GCSGC 6 cut(s) 66, 311, 392, 408, 468, 471
TseFI GTSAC 1 cut(s) 191
TseI GCWGC 1 cut(s) 111
Tsp45I GTSAC 1 cut(s) 191
Tth111I GACNNNGTC 2 cut(s) 267, 428
VpaK11BI GGWCC 2 cut(s) 447, 492
XmaJI CCTAGG 1 cut(s) 47
XmiI GTMKAC 1 cut(s) 262
XspI CTAG 3 cut(s) 48, 288, 516
ZraI GACGTC 1 cut(s) 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.