Zinc finger, C2H2 type
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 2 cut(s) | 367, 839 |
| AciI | CCGC | 5 cut(s) | 279, 309, 353, 834, 908 |
| AclWI | GGATC | 2 cut(s) | 69, 537 |
| AcsI | RAATTY | 2 cut(s) | 134, 808 |
| AcuI | CTGAAG | 1 cut(s) | 686 |
| AfaI | GTAC | 3 cut(s) | 144, 506, 921 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 199, 492, 764, 881 |
| AflIII | ACRYGT | 1 cut(s) | 787 |
| AgsI | TTSAA | 4 cut(s) | 363, 434, 592, 870 |
| AjnI | CCWGG | 2 cut(s) | 490, 640 |
| AluBI | AGCT | 3 cut(s) | 48, 100, 430 |
| AluI | AGCT | 3 cut(s) | 48, 100, 430 |
| Alw21I | GWGCWC | 1 cut(s) | 726 |
| Alw26I | GTCTC | 1 cut(s) | 601 |
| Alw44I | GTGCAC | 1 cut(s) | 722 |
| AlwI | GGATC | 2 cut(s) | 69, 537 |
| ApaLI | GTGCAC | 1 cut(s) | 722 |
| ApeKI | GCWGC | 1 cut(s) | 41 |
| ApoI | RAATTY | 2 cut(s) | 134, 808 |
| AsuC2I | CCSGG | 2 cut(s) | 759, 814 |
| AsuHPI | GGTGA | 3 cut(s) | 236, 630, 848 |
| AsuNHI | GCTAGC | 1 cut(s) | 44 |
| BaeGI | GKGCMC | 1 cut(s) | 726 |
| BauI | CACGAG | 1 cut(s) | 803 |
| Bbv12I | GWGCWC | 1 cut(s) | 726 |
| BbvI | GCAGC | 1 cut(s) | 28 |
| BccI | CCATC | 1 cut(s) | 215 |
| BciT130I | CCWGG | 2 cut(s) | 492, 642 |
| BciVI | GTATCC | 1 cut(s) | 429 |
| BcnI | CCSGG | 2 cut(s) | 759, 814 |
| BcoDI | GTCTC | 1 cut(s) | 601 |
| BfaI | CTAG | 2 cut(s) | 45, 973 |
| BfmI | CTRYAG | 1 cut(s) | 49 |
| BfuAI | ACCTGC | 2 cut(s) | 367, 839 |
| BfuI | GTATCC | 1 cut(s) | 429 |
| BglII | AGATCT | 1 cut(s) | 11 |
| BisI | GCNGC | 1 cut(s) | 42 |
| BlsI | GCNGC | 1 cut(s) | 43 |
| BmcAI | AGTACT | 1 cut(s) | 506 |
| Bme1390I | CCNGG | 4 cut(s) | 492, 642, 759, 814 |
| BmrFI | CCNGG | 4 cut(s) | 492, 642, 759, 814 |
| BmsI | GCATC | 3 cut(s) | 85, 448, 940 |
| BmtI | GCTAGC | 1 cut(s) | 48 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 656, 688 |
| BpuEI | CTTGAG | 1 cut(s) | 512 |
| BpuMI | CCSGG | 2 cut(s) | 759, 814 |
| BsaAI | YACGTR | 1 cut(s) | 823 |
| BsaJI | CCNNGG | 3 cut(s) | 491, 687, 941 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 199, 492, 764, 881 |
| Bse1I | ACTGG | 2 cut(s) | 214, 370 |
| BseBI | CCWGG | 2 cut(s) | 492, 642 |
| BseDI | CCNNGG | 3 cut(s) | 491, 687, 941 |
| BseGI | GGATG | 2 cut(s) | 37, 256 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 199, 492, 764, 881 |
| BseMII | CTCAG | 2 cut(s) | 153, 359 |
| BseNI | ACTGG | 2 cut(s) | 214, 370 |
| BseRI | GAGGAG | 1 cut(s) | 930 |
| BseSI | GKGCMC | 1 cut(s) | 726 |
| BseXI | GCAGC | 1 cut(s) | 28 |
| BsiHKAI | GWGCWC | 1 cut(s) | 726 |
| BsiSI | CCGG | 2 cut(s) | 759, 813 |
| BslFI | GGGAC | 1 cut(s) | 723 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 199, 492, 764, 881 |
| BsmAI | GTCTC | 1 cut(s) | 601 |
| BsmFI | GGGAC | 1 cut(s) | 723 |
| BsmI | GAATGC | 1 cut(s) | 527 |
| Bsp1286I | GDGCHC | 1 cut(s) | 726 |
| Bsp143I | GATC | 3 cut(s) | 11, 61, 542 |
| BspACI | CCGC | 5 cut(s) | 279, 309, 353, 834, 908 |
| BspCNI | CTCAG | 2 cut(s) | 152, 358 |
| BspHI | TCATGA | 1 cut(s) | 622 |
| BspMI | ACCTGC | 2 cut(s) | 367, 839 |
| BspOI | GCTAGC | 1 cut(s) | 48 |
| BspPI | GGATC | 2 cut(s) | 69, 537 |
| BspQI | GCTCTTC | 1 cut(s) | 679 |
| BsrI | ACTGG | 2 cut(s) | 214, 370 |
| BssECI | CCNNGG | 3 cut(s) | 491, 687, 941 |
| BssMI | GATC | 3 cut(s) | 11, 61, 542 |
| BssSI | CACGAG | 1 cut(s) | 803 |
| BssT1I | CCWWGG | 1 cut(s) | 687 |
| Bst2BI | CACGAG | 1 cut(s) | 803 |
| Bst2UI | CCWGG | 2 cut(s) | 492, 642 |
| Bst4CI | ACNGT | 2 cut(s) | 194, 566 |
| Bst6I | CTCTTC | 1 cut(s) | 679 |
| BstBAI | YACGTR | 1 cut(s) | 823 |
| BstC8I | GCNNGC | 2 cut(s) | 46, 351 |
| BstDEI | CTNAG | 3 cut(s) | 139, 345, 582 |
| BstF5I | GGATG | 2 cut(s) | 37, 256 |
| BstKTI | GATC | 3 cut(s) | 14, 64, 545 |
| BstMAI | GTCTC | 1 cut(s) | 601 |
| BstMBI | GATC | 3 cut(s) | 11, 61, 542 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 436 |
| BstNI | CCWGG | 2 cut(s) | 492, 642 |
| BstNSI | RCATGY | 2 cut(s) | 167, 791 |
| BstSCI | CCNGG | 4 cut(s) | 490, 640, 757, 812 |
| BstSFI | CTRYAG | 1 cut(s) | 49 |
| BstSLI | GKGCMC | 1 cut(s) | 726 |
| BstV1I | GCAGC | 1 cut(s) | 28 |
| BstX2I | RGATCY | 2 cut(s) | 11, 542 |
| BstYI | RGATCY | 2 cut(s) | 11, 542 |
| BsuI | GTATCC | 1 cut(s) | 429 |
| BtgZI | GCGATG | 1 cut(s) | 234 |
| BtsCI | GGATG | 2 cut(s) | 37, 256 |
| BtsI | GCAGTG | 1 cut(s) | 726 |
| BtsIMutI | CAGTG | 1 cut(s) | 726 |
| BveI | ACCTGC | 2 cut(s) | 367, 839 |
| Cac8I | GCNNGC | 2 cut(s) | 46, 351 |
| CciI | TCATGA | 1 cut(s) | 622 |
| Csp6I | GTAC | 3 cut(s) | 143, 505, 920 |
| CviAII | CATG | 7 cut(s) | 164, 452, 623, 712, 769, 788, 934 |
| CviJI | RGCY | 8 cut(s) | 48, 100, 349, 410, 430, 490, 692, 753 |
| CviKI_1 | RGCY | 8 cut(s) | 48, 100, 349, 410, 430, 490, 692, 753 |
| CviQI | GTAC | 3 cut(s) | 143, 505, 920 |
| DdeI | CTNAG | 3 cut(s) | 139, 345, 582 |
| DpnI | GATC | 3 cut(s) | 13, 63, 544 |
| DpnII | GATC | 3 cut(s) | 11, 61, 542 |
| Eam1104I | CTCTTC | 1 cut(s) | 679 |
| EarI | CTCTTC | 1 cut(s) | 679 |
| EciI | GGCGGA | 1 cut(s) | 324 |
| Eco130I | CCWWGG | 1 cut(s) | 687 |
| Eco57I | CTGAAG | 1 cut(s) | 686 |
| EcoRI | GAATTC | 2 cut(s) | 134, 808 |
| EcoRII | CCWGG | 2 cut(s) | 490, 640 |
| EcoT14I | CCWWGG | 1 cut(s) | 687 |
| ErhI | CCWWGG | 1 cut(s) | 687 |
| FaeI | CATG | 7 cut(s) | 167, 455, 626, 715, 772, 791, 937 |
| FaqI | GGGAC | 1 cut(s) | 723 |
| FatI | CATG | 7 cut(s) | 163, 451, 622, 711, 768, 787, 933 |
| Fnu4HI | GCNGC | 1 cut(s) | 42 |
| FokI | GGATG | 2 cut(s) | 44, 263 |
| Fsp4HI | GCNGC | 1 cut(s) | 42 |
| FspBI | CTAG | 2 cut(s) | 45, 973 |
| GluI | GCNGC | 1 cut(s) | 42 |
| HapII | CCGG | 2 cut(s) | 759, 813 |
| Hin1II | CATG | 7 cut(s) | 167, 455, 626, 715, 772, 791, 937 |
| HinfI | GANTC | 6 cut(s) | 341, 446, 464, 619, 632, 930 |
| HpaII | CCGG | 2 cut(s) | 759, 813 |
| HphI | GGTGA | 3 cut(s) | 236, 630, 848 |
| Hpy166II | GTNNAC | 2 cut(s) | 724, 884 |
| Hpy188I | TCNGA | 5 cut(s) | 114, 372, 487, 705, 794 |
| Hpy188III | TCNNGA | 4 cut(s) | 443, 604, 623, 870 |
| Hpy8I | GTNNAC | 2 cut(s) | 724, 884 |
| HpyAV | CCTTC | 5 cut(s) | 52, 109, 283, 293, 432 |
| HpyCH4III | ACNGT | 2 cut(s) | 194, 566 |
| HpyCH4IV | ACGT | 2 cut(s) | 736, 822 |
| HpyCH4V | TGCA | 4 cut(s) | 126, 387, 724, 953 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 436 |
| HpyF3I | CTNAG | 3 cut(s) | 139, 345, 582 |
| HpySE526I | ACGT | 2 cut(s) | 736, 822 |
| Hsp92II | CATG | 7 cut(s) | 167, 455, 626, 715, 772, 791, 937 |
| Kzo9I | GATC | 3 cut(s) | 11, 61, 542 |
| LguI | GCTCTTC | 1 cut(s) | 679 |
| Lsp1109I | GCAGC | 1 cut(s) | 28 |
| LweI | GCATC | 3 cut(s) | 85, 448, 940 |
| MaeI | CTAG | 2 cut(s) | 45, 973 |
| MaeII | ACGT | 2 cut(s) | 736, 822 |
| MaeIII | GTNAC | 2 cut(s) | 531, 901 |
| MalI | GATC | 3 cut(s) | 13, 63, 544 |
| MboI | GATC | 3 cut(s) | 11, 61, 542 |
| MboII | GAAGA | 7 cut(s) | 71, 530, 641, 666, 692, 869, 968 |
| MflI | RGATCY | 2 cut(s) | 11, 542 |
| MhlI | GDGCHC | 1 cut(s) | 726 |
| MluCI | AATT | 4 cut(s) | 134, 393, 458, 808 |
| MlyI | GAGTC | 3 cut(s) | 458, 613, 939 |
| MnlI | CCTC | 9 cut(s) | 48, 466, 536, 646, 658, 872, 885, 948, 951 |
| MseI | TTAA | 3 cut(s) | 105, 168, 414 |
| MslI | CAYNNNNRTG | 1 cut(s) | 183 |
| MspI | CCGG | 2 cut(s) | 759, 813 |
| MspR9I | CCNGG | 4 cut(s) | 492, 642, 759, 814 |
| Mva1269I | GAATGC | 1 cut(s) | 527 |
| MvaI | CCWGG | 2 cut(s) | 492, 642 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 436 |
| NciI | CCSGG | 2 cut(s) | 759, 814 |
| NdeII | GATC | 3 cut(s) | 11, 61, 542 |
| NheI | GCTAGC | 1 cut(s) | 44 |
| NlaIII | CATG | 7 cut(s) | 167, 455, 626, 715, 772, 791, 937 |
| NmuCI | GTSAC | 1 cut(s) | 531 |
| NspI | RCATGY | 2 cut(s) | 167, 791 |
| PagI | TCATGA | 1 cut(s) | 622 |
| PciI | ACATGT | 1 cut(s) | 787 |
| PciSI | GCTCTTC | 1 cut(s) | 679 |
| PctI | GAATGC | 1 cut(s) | 527 |
| PfeI | GAWTC | 3 cut(s) | 341, 446, 632 |
| PkrI | GCNGC | 1 cut(s) | 43 |
| PleI | GAGTC | 3 cut(s) | 458, 613, 938 |
| PpsI | GAGTC | 3 cut(s) | 458, 613, 938 |
| Ppu21I | YACGTR | 1 cut(s) | 823 |
| PscI | ACATGT | 1 cut(s) | 787 |
| Psp6I | CCWGG | 2 cut(s) | 490, 640 |
| PspGI | CCWGG | 2 cut(s) | 490, 640 |
| PsuI | RGATCY | 2 cut(s) | 11, 542 |
| RsaI | GTAC | 3 cut(s) | 144, 506, 921 |
| RsaNI | GTAC | 3 cut(s) | 143, 505, 920 |
| RseI | CAYNNNNRTG | 1 cut(s) | 183 |
| SapI | GCTCTTC | 1 cut(s) | 679 |
| SaqAI | TTAA | 3 cut(s) | 105, 168, 414 |
| SatI | GCNGC | 1 cut(s) | 42 |
| Sau3AI | GATC | 3 cut(s) | 11, 61, 542 |
| ScaI | AGTACT | 1 cut(s) | 506 |
| SchI | GAGTC | 3 cut(s) | 458, 613, 939 |
| ScrFI | CCNGG | 4 cut(s) | 492, 642, 759, 814 |
| SduI | GDGCHC | 1 cut(s) | 726 |
| SetI | ASST | 8 cut(s) | 50, 102, 381, 432, 739, 825, 833, 883 |
| SfaNI | GCATC | 3 cut(s) | 85, 448, 940 |
| SfcI | CTRYAG | 1 cut(s) | 49 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 183 |
| SmlI | CTYRAG | 1 cut(s) | 527 |
| SmoI | CTYRAG | 1 cut(s) | 527 |
| Sse9I | AATT | 4 cut(s) | 134, 393, 458, 808 |
| SsiI | CCGC | 5 cut(s) | 279, 309, 353, 834, 908 |
| SspMI | CTAG | 2 cut(s) | 45, 973 |
| StyD4I | CCNGG | 4 cut(s) | 490, 640, 757, 812 |
| StyI | CCWWGG | 1 cut(s) | 687 |
| TaaI | ACNGT | 2 cut(s) | 194, 566 |
| TaiI | ACGT | 2 cut(s) | 739, 825 |
| TaqI | TCGA | 2 cut(s) | 677, 846 |
| TaqII | GACCGA | 1 cut(s) | 889 |
| TasI | AATT | 4 cut(s) | 134, 393, 458, 808 |
| TatI | WGTACW | 2 cut(s) | 142, 504 |
| TfiI | GAWTC | 3 cut(s) | 341, 446, 632 |
| Tru1I | TTAA | 3 cut(s) | 105, 168, 414 |
| Tru9I | TTAA | 3 cut(s) | 105, 168, 414 |
| TscAI | CASTG | 1 cut(s) | 726 |
| TseFI | GTSAC | 1 cut(s) | 531 |
| TseI | GCWGC | 1 cut(s) | 41 |
| Tsp45I | GTSAC | 1 cut(s) | 531 |
| TspDTI | ATGAA | 5 cut(s) | 312, 515, 642, 728, 870 |
| TspGWI | ACGGA | 1 cut(s) | 631 |
| TspRI | CASTG | 1 cut(s) | 726 |
| VneI | GTGCAC | 1 cut(s) | 722 |
| XapI | RAATTY | 2 cut(s) | 134, 808 |
| XceI | RCATGY | 2 cut(s) | 167, 791 |
| XspI | CTAG | 2 cut(s) | 45, 973 |
| ZrmI | AGTACT | 1 cut(s) | 506 |