Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000007

Orthogroup ID

OG0000543

Physical Location & Seq

Genomic Coordinates Forward (+)

23267891 .. 23270734

Gene Structure 2844 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug07G0251500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2844 bp

ATGGCGGAGAGTGCAGTAAAGTTTCTGCTTGACAAGCTTGCACCTCTTTTCGAAAATGAGTTGCAACTTTTGAGAGGGGTTAGGGAAGAAATTTTGTATCTAAGAGGAGAACTGGAGCGCATGACGGCTTTCCTGAGGGTTGCTGATGCATATGAGGAGAGTGATGCGGAACTCAAAGTATGGGTTAAACAACTAAGAGACATTGCTCACGAAAGTGAAGATGTTCTTGATGAATTTACCCTTCTGCAAGCACATGATCATGGGGAGGGATTATATGGTTCAATCCATAAGCTTTCTTGCTGCATCAAGAATACAAAAGCTCGATATCGAATTGCTTCGGAGTTACGAGCCATAAACTCCAGGATCAAAAAAATCTCTGAGGTTCAAAAGCGACTCCGCCACAAATTCAATATGGCTGAACAAGGCTCAAGCTCCAAAAGTGCAGGTTACCTGTTGGAGGACCATCGAAGTGATGCTTTACTTCTAGAGAAAACTGATATAGTGGGCATAGACGAGCCTATCAAGCAGATGGTTGGGAGGCTGGTCAATGGTGGTAGTGGACGTGAGGTAGTTTCTGTGGCAGGAATGGGAGGAATGGGGAAAACTACCCTGGCGAAGCAAGTCTACGATGCAGCAGAAGTGAAGAAACATTTCAAATTACGTTCTTGGATCACTTTTACTCAGTCTTTCAAGCTAGTGGAACTTCTCAAAGACATCGTTCAACAGCTCCACCAAGCAATCCGAAGTCCAGTTCCACAAGAAATCAATAGCATGAGTACCAACCAGCTGAAAACAATAATTAAAGACTTCCTACAGAAGAGGAGGTATCTCATTGTCTTGGATGATGTGTGGCACTTATATGGATGGGATGCCTTGAAGTATGCACTGCCTAATAATATTTACGGCAGCAGAGTGATACTAACTACTCGAAATGCAGATATAGCCTCCACAACAAGCACAGAATCTGGAGGTGAAGTCTATACTTTGGAGCCCTTGCCCCCAGTGGAGTCATGGGAGCTCCTGTGCAAGAAGACATTCCAAGGGAATTCCTGCCCTCCTCATTTAGAGAAAATCTGTAACTCTGTTCTGAGAAAGTGTGAGGGGCTACCCCTTGCTATTGTGGCAATTAGTGGAGTTCTGGCTGCTAAAGATAAGCGCAGAATTGATGAGTGGGAAATTGTTGGCCACAGTCTCGGGGCTGAAATTGAGGGCAATGACAAACTGCAGGACTTGAAGAAAGTACTTTCACTCAGTTTCAATGATTTACCATACTATCTAAAATCGTGCTTCTTGTACCTGAGCATCTTTCCAGAGGACCATCTAATTAAGCATATGAGGCTTATTCGATTATGGATGGCAGAGGGCTTCATTGAAGAAAAACAAGGAAAGACATTGGAAGAGGTTGCAGAGGATTACCTCAATGAACTATTGAATAGGAGCATGATTCAAGCAGCAGAGACAACAGCTGATGGAAGGGTCACAAAATTTCGTATCCATGATCTCTTCCGTGAAATTATCATCTCCAAGACAAGAAATCAGAACTTTGCCACAATAGCCAAAGAGCAGAATGTAACATTGCCAGAAAAAATCCGTCGCCTGTCAATACATAACACACTGCAATATGTTCAGACAAACAGGTGTGCTTCTCAACTACGTTCTCTTTTTATGTTCACAGTTGCTGAGAAGCCATCACTACAAAGACTATTCCCAGCCGGCTTTGCGCTGCTTAATGTGTTAGATTTGCAAAGTACACCTTTAAATGTTTTCCCAGTTGAAGTTGTGAATCTTTACTTTTTAAAGTATCTGAGCTTGAGAGATACCAGGGTAAAAACCATTCCAAGATTTATAGGGAAGCTTCAGAACCTGGAAACTTTGGATCTTAAGCATTCTCGAGTTACTGAACTCCCAGTTGAAATTCTGAAACTCCAACATTTGCGCTATCTCTTGGTATATCGTTATGAGTTTGTTCCTTACGAAGATTTTCACTCAACATATGGTTTTAAGGTCCTTGCAAAAATAGGAGCTTTGAAATCCCTTCAAAAGCTATGTTTCATTGAGGTGAACCAAGGTGGCAGCGCATTATTGAGAGAGCTGGGGAAACTAGTTCAGTTGAGAAGGTTAGGCATTGTTAAGATGAGGAAAAAAGATGGCAAGGCTCTTTCTTCATCCATTGAAAAGTTGTCCAAACTTTATGCATTGTCCATAACTTCAGTGGAAGAAGACGAGATCATTGATTTGCAGCACCTGTGTTCTCCTCCTCTATTGCTTCAGCATTTGTACTTGCGAGGCAGATTGGAAGCATTACCTCACTGGATACCTTCTCTCCATAGCCTCGTCAAGTTGTATTTGAAATGGAGCAGGTTAAGGGATGATCCACTTGCATTCCTTCAGTATTTGCCCAATCTAGTACATCTTGAGTTGTGTCAGGTTTTTGAAGGAGACACACTATGTTTTGGAGCCGGCGGATTTAAGAAGCTCAAGCATTTATGCCTCGATAAATCTGATGAACTTCGATGCATAGAGATGGAGGTGGGAACAATGCCTTGTATTGAAAATCTAATCATCCGGCGCTGTAAATCACTGGAGAAAGTCCCATCAGGCATTGAACATCTCATCAAACTAAAGTTGCTTAAGTTTTTTGATATGCCAGAAAAACTAATCAAGACTCTACTTCCACATGAGCATGGCAATGATTATTGGAAAGTTGCGCATGTCCCAGAAGTTTATGTCACCTACTTGAGAGAGTTTGGGTGGGAAGTATTCTCATTAGAGGGCTTGAGTGAGACACAAATCTCTTCTCAACCCAGTTCTGTCATGAAGAGCATTGGAACTGGTTGGAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000182 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0006355 GO:0008097 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0019843 GO:0022613 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0042254 GO:0042273 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0070013 GO:0071840 GO:0080084 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:1990837 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR zf-C2H2

Protein Analysis

947

Amino Acids

108.49

Weight (kDa)

8.74

Isoelectric Point (pI)

42.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rx_N	PF18052	5 - 87	2.3e-23	Rx N-terminal domain
NB-ARC	PF00931	172 - 350	3.6e-41	NB-ARC domain
AAA_16	PF13191	183 - 283	6.2e-07	AAA ATPase domain
WHD_DRP	PF23559	435 - 506	1.6e-26	Disease resistance protein Winged helix domain
LRR_14	PF23598	550 - 878	1.6e-45	Leucine-rich repeat region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000543)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G72050 AT1G72050 AT1G72050 AT1G72050
fragaria_vesca	FvH4_1g04960 FvH4_1g04960 FvH4_1g04960 FvH4_1g04960 FvH4_1g04960 FvH4_1g04960 FvH4_1g04960 FvH4_1g04960 FvH4_1g04960 FvH4_1g04960 FvH4_1g04960 FvH4_1g27520 FvH4_1g27520 FvH4_4g26190 FvH4_4g26190 FvH4_4g26190 FvH4_4g26190 FvH4_4g26190 FvH4_4g26190 FvH4_4g26190 FvH4_4g26190 FvH4_4g26190 FvH4_4g26190 FvH4_4g26190 FvH4_4g26190
malus_domestica	MD02G1048300.v1.1 MD15G1186600.v1.1 MD15G1411000.v1.1
prunus_persica	Prupe.1G551600_v2.0.a1 Prupe.1G551600_v2.0.a1 Prupe.1G551600_v2.0.a1 Prupe.1G551600_v2.0.a1 Prupe.7G229900_v2.0.a1 Prupe.7G229900_v2.0.a1
pyrus_communis	pycom02g04050 pycom15g36580
rosa_chinensis	RchiOBHm_Chr2g0090581 RchiOBHm_Chr2g0090591 RchiOBHm_Chr2g0120581 RchiOBHm_Chr5g0055971 RchiOBHm_Chr7g0228681 RchiOBHm_Chr7g0228721 RchiOBHm_Chr7g0228771
rosa_laevigata	RLG00000001547 RLG00000016122 RLG00000035039
rosa_multiflora	Rmu_co8222532.1_g000001 Rmu_co8474195.1_g000001 Rmu_sc0001581.1_g000005 Rmu_sc0004514.1_g000033 Rmu_sc0015624.1_g000002
rosa_roxburghii	Rroxscaffold_1G00024300 Rroxscaffold_2G00151010 Rroxscaffold_3G00230860 Rroxscaffold_3G00230910
rosa_rugosa	Rorug02G0009300 Rorug02G0009300 Rorug05G0296700 Rorug07G0251500 Rorug07G0251600 Rorug07G0251700 Rorug07G0251800
rosa_samantha	Rh2AG054400 Rh2BG053700 Rh2CG055900 Rh2DG054800 Rh5BG378200 Rh5CG401600 Rh7AG403700 Rh7AG404000 Rh7AG404600 Rh7AG405100 Rh7BG384300 Rh7CG422800 Rh7DG399300
rosa_wichuraiana	Rw2G004740 Rw2G022930 Rw5G034520 Rw7G033550 Rw7G033570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	2709
Acc36I	ACCTGC	2 cut(s)	434, 2349
AccI	GTMKAC	1 cut(s)	624
AciI	CCGC	4 cut(s)	5, 167, 397, 2463
AclWI	GGATC	4 cut(s)	371, 677, 1884, 2366
AcoI	YGGCCR	1 cut(s)	1183
AcsI	RAATTY	6 cut(s)	90, 233, 404, 1045, 1484, 1914
AcuI	CTGAAG	4 cut(s)	1841, 2193, 2252, 2372
AfaI	GTAC	6 cut(s)	778, 1242, 1295, 1750, 2279, 2409
AfiI	CCNNNNNNNGG	1 cut(s)	457
AflII	CTTAAG	2 cut(s)	1880, 2630
AhlI	ACTAGT	1 cut(s)	2101
AjiI	CACGTC	1 cut(s)	563
AjnI	CCWGG	4 cut(s)	359, 609, 1820, 1863
AleI	CACNNNNGTG	1 cut(s)	213
Alw21I	GWGCWC	1 cut(s)	1020
Alw26I	GTCTC	5 cut(s)	192, 1196, 1451, 2433, 2777
AlwI	GGATC	4 cut(s)	371, 677, 1884, 2366
AlwNI	CAGNNNCTG	4 cut(s)	965, 1679, 1864, 2245
Ama87I	CYCGRG	2 cut(s)	1193, 1890
AoxI	GGCC	1 cut(s)	1183
ApeKI	GCWGC	8 cut(s)	300, 632, 906, 1142, 1451, 1723, 2073, 2239
ApoI	RAATTY	6 cut(s)	90, 233, 404, 1045, 1484, 1914
Asp700I	GAANNNNTTC	5 cut(s)	222, 334, 650, 1034, 1980
AspLEI	GCGC	7 cut(s)	120, 1158, 1723, 1938, 2078, 2571, 2710
AspS9I	GGNCC	3 cut(s)	460, 1315, 2005
AsuHPI	GGTGA	3 cut(s)	983, 2071, 2722
AsuII	TTCGAA	1 cut(s)	51
AvaI	CYCGRG	2 cut(s)	1193, 1890
AvaII	GGWCC	3 cut(s)	460, 1315, 2005
AxyI	CCTNAGG	1 cut(s)	134
BaeI	ACNNNNGTAYC	2 cut(s)	1809, 1842
BalI	TGGCCA	1 cut(s)	1185
BanII	GRGCYC	2 cut(s)	993, 1020
BarI	GAAGNNNNNNTAC	2 cut(s)	608, 640
BbsI	GAAGAC	2 cut(s)	1037, 2226
Bbv12I	GWGCWC	1 cut(s)	1020
BbvI	GCAGC	8 cut(s)	287, 644, 918, 1129, 1463, 1710, 2085, 2251
BceAI	ACGGC	2 cut(s)	141, 919
BciT130I	CCWGG	4 cut(s)	361, 611, 1822, 1865
BciVI	GTATCC	2 cut(s)	1502, 2307
BclI	TGATCA	1 cut(s)	256
BcoDI	GTCTC	5 cut(s)	192, 1196, 1451, 2433, 2777
BcuI	ACTAGT	1 cut(s)	2101
BfaI	CTAG	4 cut(s)	485, 695, 2102, 2405
BfmI	CTRYAG	2 cut(s)	812, 1223
BfoI	RGCGCY	1 cut(s)	2572
BfrI	CTTAAG	2 cut(s)	1880, 2630
BfuAI	ACCTGC	2 cut(s)	434, 2349
BfuI	GTATCC	2 cut(s)	1502, 2307
BisI	GCNGC	8 cut(s)	301, 633, 907, 1143, 1452, 1724, 2074, 2240
BlsI	GCNGC	8 cut(s)	302, 634, 908, 1144, 1453, 1725, 2075, 2241
BmcAI	AGTACT	1 cut(s)	1242
Bme1390I	CCNGG	4 cut(s)	361, 611, 1822, 1865
Bme18I	GGWCC	3 cut(s)	460, 1315, 2005
BmeT110I	CYCGRG	2 cut(s)	1193, 1890
BmgBI	CACGTC	1 cut(s)	563
BmgT120I	GGNCC	3 cut(s)	460, 1315, 2005
BmiI	GGNNCC	2 cut(s)	990, 2458
BmrFI	CCNGG	4 cut(s)	361, 611, 1822, 1865
BmrI	ACTGGG	4 cut(s)	995, 1763, 1901, 2799
BmsI	GCATC	8 cut(s)	136, 154, 312, 463, 619, 859, 1311, 2504
BmuI	ACTGGG	4 cut(s)	995, 1763, 1901, 2799
BpiI	GAAGAC	2 cut(s)	1037, 2226
BpmI	CTGGAG	4 cut(s)	134, 343, 987, 2603
Bpu10I	CCTNAGC	1 cut(s)	1298
Bpu14I	TTCGAA	1 cut(s)	51
BpuEI	CTTGAG	6 cut(s)	412, 1831, 2435, 2462, 2758, 2797
BsaJI	CCNNGG	4 cut(s)	609, 1039, 1821, 2065
BsaXI	ACNNNNNCTCC	6 cut(s)	960, 990, 1007, 1037, 2307, 2337
Bsc4I	CCNNNNNNNGG	1 cut(s)	457
Bse118I	RCCGGY	2 cut(s)	1712, 2459
Bse1I	ACTGG	9 cut(s)	117, 749, 1001, 1769, 1907, 2315, 2586, 2805, 2836
Bse21I	CCTNAGG	1 cut(s)	134
Bse3DI	GCAATG	4 cut(s)	201, 1219, 1574, 2695
BseBI	CCWGG	4 cut(s)	361, 611, 1822, 1865
BseDI	CCNNGG	4 cut(s)	609, 1039, 1821, 2065
BseGI	GGATG	7 cut(s)	847, 869, 874, 1359, 2165, 2374, 2562
BseLI	CCNNNNNNNGG	1 cut(s)	457
BseMI	GCAATG	4 cut(s)	201, 1219, 1574, 2695
BseMII	CTCAG	8 cut(s)	125, 369, 695, 1079, 1264, 1289, 1671, 1796
BseNI	ACTGG	9 cut(s)	117, 749, 1001, 1769, 1907, 2315, 2586, 2805, 2836
BseRI	GAGGAG	6 cut(s)	120, 170, 835, 1047, 2244, 2247
BseXI	GCAGC	8 cut(s)	287, 644, 918, 1129, 1463, 1710, 2085, 2251
BseYI	CCCAGC	2 cut(s)	1708, 2092
BsgI	GTGCAG	2 cut(s)	33, 462
BshFI	GGCC	1 cut(s)	1185
BsiHKAI	GWGCWC	1 cut(s)	1020
BsiHKCI	CYCGRG	2 cut(s)	1193, 1890
BsiSI	CCGG	3 cut(s)	1713, 2460, 2566
BslFI	GGGAC	2 cut(s)	2576, 2699
BslI	CCNNNNNNNGG	1 cut(s)	457
BsmAI	GTCTC	5 cut(s)	192, 1196, 1451, 2433, 2777
BsmFI	GGGAC	2 cut(s)	2576, 2699
BsmI	GAATGC	2 cut(s)	1885, 2381
BsnI	GGCC	1 cut(s)	1185
BsoBI	CYCGRG	2 cut(s)	1193, 1890
Bsp119I	TTCGAA	1 cut(s)	51
Bsp1286I	GDGCHC	2 cut(s)	993, 1020
Bsp143I	GATC	7 cut(s)	256, 363, 669, 1498, 1876, 2226, 2371
BspACI	CCGC	4 cut(s)	5, 167, 397, 2463
BspANI	GGCC	1 cut(s)	1185
BspCNI	CTCAG	8 cut(s)	126, 370, 694, 1080, 1263, 1290, 1672, 1797
BspHI	TCATGA	1 cut(s)	2814
BspLI	GGNNCC	2 cut(s)	990, 2458
BspMAI	CTGCAG	1 cut(s)	1227
BspMI	ACCTGC	2 cut(s)	434, 2349
BspPI	GGATC	4 cut(s)	371, 677, 1884, 2366
BspQI	GCTCTTC	1 cut(s)	2813
BspT104I	TTCGAA	1 cut(s)	51
BspTI	CTTAAG	2 cut(s)	1880, 2630
BsrDI	GCAATG	4 cut(s)	201, 1219, 1574, 2695
BsrFI	RCCGGY	2 cut(s)	1712, 2459
BsrI	ACTGG	9 cut(s)	117, 749, 1001, 1769, 1907, 2315, 2586, 2805, 2836
BssAI	RCCGGY	2 cut(s)	1712, 2459
BssECI	CCNNGG	4 cut(s)	609, 1039, 1821, 2065
BssMI	GATC	7 cut(s)	256, 363, 669, 1498, 1876, 2226, 2371
BssT1I	CCWWGG	2 cut(s)	1039, 2065
Bst2UI	CCWGG	4 cut(s)	361, 611, 1822, 1865
Bst4CI	ACNGT	2 cut(s)	1190, 1675
Bst6I	CTCTTC	5 cut(s)	812, 1392, 1508, 2800, 2813
BstAFI	CTTAAG	2 cut(s)	1880, 2630
BstBI	TTCGAA	1 cut(s)	51
BstC8I	GCNNGC	4 cut(s)	39, 249, 1714, 2461
BstEII	GGTNACC	1 cut(s)	446
BstENI	CCTNNNNNAGG	1 cut(s)	455
BstF5I	GGATG	7 cut(s)	847, 869, 874, 1359, 2165, 2374, 2562
BstH2I	RGCGCY	1 cut(s)	2572
BstHHI	GCGC	7 cut(s)	120, 1158, 1723, 1938, 2078, 2571, 2710
BstKTI	GATC	7 cut(s)	259, 366, 672, 1501, 1879, 2229, 2374
BstMAI	GTCTC	5 cut(s)	192, 1196, 1451, 2433, 2777
BstMBI	GATC	7 cut(s)	256, 363, 669, 1498, 1876, 2226, 2371
BstMWI	GCNNNNNNNGC	8 cut(s)	11, 34, 523, 941, 1121, 1336, 1553, 1718
BstNI	CCWGG	4 cut(s)	361, 611, 1822, 1865
BstNSI	RCATGY	1 cut(s)	2714
BstPI	GGTNACC	1 cut(s)	446
BstSCI	CCNGG	4 cut(s)	359, 609, 1820, 1863
BstSFI	CTRYAG	2 cut(s)	812, 1223
BstV1I	GCAGC	8 cut(s)	287, 644, 918, 1129, 1463, 1710, 2085, 2251
BstV2I	GAAGAC	2 cut(s)	1037, 2226
BstX2I	RGATCY	1 cut(s)	1876
BstYI	RGATCY	1 cut(s)	1876
Bsu36I	CCTNAGG	1 cut(s)	134
BsuI	GTATCC	2 cut(s)	1502, 2307
BsuRI	GGCC	1 cut(s)	1185
BtrI	CACGTC	1 cut(s)	563
BtsCI	GGATG	7 cut(s)	847, 869, 874, 1359, 2165, 2374, 2562
BtsI	GCAGTG	2 cut(s)	884, 1613
BtsIMutI	CAGTG	6 cut(s)	884, 1008, 1613, 2217, 2308, 2579
BveI	ACCTGC	2 cut(s)	434, 2349
Cac8I	GCNNGC	4 cut(s)	39, 249, 1714, 2461
CaiI	CAGNNNCTG	4 cut(s)	965, 1679, 1864, 2245
CciI	TCATGA	1 cut(s)	2814
CfoI	GCGC	7 cut(s)	120, 1158, 1723, 1938, 2078, 2571, 2710
Cfr10I	RCCGGY	2 cut(s)	1712, 2459
Cfr13I	GGNCC	3 cut(s)	460, 1315, 2005
Csp6I	GTAC	6 cut(s)	777, 1241, 1294, 1749, 2278, 2408
CviQI	GTAC	6 cut(s)	777, 1241, 1294, 1749, 2278, 2408
DpnI	GATC	7 cut(s)	258, 365, 671, 1500, 1878, 2228, 2373
DpnII	GATC	7 cut(s)	256, 363, 669, 1498, 1876, 2226, 2371
DraI	TTTAAA	2 cut(s)	1758, 1797
EaeI	YGGCCR	1 cut(s)	1183
Eam1104I	CTCTTC	5 cut(s)	812, 1392, 1508, 2800, 2813
EarI	CTCTTC	5 cut(s)	812, 1392, 1508, 2800, 2813
EciI	GGCGGA	3 cut(s)	20, 386, 2478
Ecl136II	GAGCTC	1 cut(s)	1018
Eco130I	CCWWGG	2 cut(s)	1039, 2065
Eco24I	GRGCYC	2 cut(s)	993, 1020
Eco32I	GATATC	1 cut(s)	326
Eco47I	GGWCC	3 cut(s)	460, 1315, 2005
Eco53kI	GAGCTC	1 cut(s)	1018
Eco57I	CTGAAG	4 cut(s)	1841, 2193, 2252, 2372
Eco81I	CCTNAGG	1 cut(s)	134
Eco88I	CYCGRG	2 cut(s)	1193, 1890
Eco91I	GGTNACC	1 cut(s)	446
EcoICRI	GAGCTC	1 cut(s)	1018
EcoNI	CCTNNNNNAGG	1 cut(s)	455
EcoO109I	RGGNCCY	1 cut(s)	2005
EcoO65I	GGTNACC	1 cut(s)	446
EcoRI	GAATTC	1 cut(s)	1045
EcoRII	CCWGG	4 cut(s)	359, 609, 1820, 1863
EcoRV	GATATC	1 cut(s)	326
EcoT14I	CCWWGG	2 cut(s)	1039, 2065
EcoT22I	ATGCAT	3 cut(s)	151, 2197, 2519
EcoT38I	GRGCYC	2 cut(s)	993, 1020
ErhI	CCWWGG	2 cut(s)	1039, 2065
FalI	AAGNNNNNCTT	6 cut(s)	210, 242, 460, 492, 1738, 1770
FaqI	GGGAC	2 cut(s)	2576, 2699
FauNDI	CATATG	3 cut(s)	151, 1332, 1993
FbaI	TGATCA	1 cut(s)	256
FblI	GTMKAC	1 cut(s)	624
Fnu4HI	GCNGC	8 cut(s)	301, 633, 907, 1143, 1452, 1724, 2074, 2240
FokI	GGATG	7 cut(s)	854, 876, 881, 1366, 2152, 2381, 2549
FriOI	GRGCYC	2 cut(s)	993, 1020
Fsp4HI	GCNGC	8 cut(s)	301, 633, 907, 1143, 1452, 1724, 2074, 2240
FspBI	CTAG	4 cut(s)	485, 695, 2102, 2405
FspI	TGCGCA	1 cut(s)	2709
GlaI	GCGC	7 cut(s)	119, 1157, 1722, 1937, 2077, 2570, 2709
GluI	GCNGC	8 cut(s)	301, 633, 907, 1143, 1452, 1724, 2074, 2240
GsaI	CCCAGC	2 cut(s)	1712, 2096
GsuI	CTGGAG	4 cut(s)	134, 343, 987, 2603
HaeII	RGCGCY	1 cut(s)	2572
HaeIII	GGCC	1 cut(s)	1185
HapII	CCGG	3 cut(s)	1713, 2460, 2566
HhaI	GCGC	7 cut(s)	120, 1158, 1723, 1938, 2078, 2571, 2710
Hin6I	GCGC	7 cut(s)	118, 1156, 1721, 1936, 2076, 2569, 2708
HinP1I	GCGC	7 cut(s)	118, 1156, 1721, 1936, 2076, 2569, 2708
HindIII	AAGCTT	3 cut(s)	35, 290, 1853
HinfI	GANTC	6 cut(s)	393, 962, 1007, 1444, 1783, 2665
HpaII	CCGG	3 cut(s)	1713, 2460, 2566
HphI	GGTGA	3 cut(s)	983, 2071, 2722
Hpy166II	GTNNAC	5 cut(s)	560, 625, 1671, 1751, 2062
Hpy8I	GTNNAC	5 cut(s)	560, 625, 1671, 1751, 2062
Hpy99I	CGWCG	1 cut(s)	1596
HpyAV	CCTTC	7 cut(s)	251, 1467, 2045, 2109, 2328, 2396, 2429
HpyCH4III	ACNGT	2 cut(s)	1190, 1675
HpyCH4IV	ACGT	3 cut(s)	562, 661, 1654
HpyF10VI	GCNNNNNNNGC	8 cut(s)	11, 34, 523, 941, 1121, 1336, 1553, 1718
HpySE526I	ACGT	3 cut(s)	562, 661, 1654
HspAI	GCGC	7 cut(s)	118, 1156, 1721, 1936, 2076, 2569, 2708
KroI	GCCGGC	2 cut(s)	1712, 2459
KroNI	GCCGGC	2 cut(s)	1714, 2461
Ksp22I	TGATCA	1 cut(s)	256
Kzo9I	GATC	7 cut(s)	256, 363, 669, 1498, 1876, 2226, 2371
LguI	GCTCTTC	1 cut(s)	2813
Lsp1109I	GCAGC	8 cut(s)	287, 644, 918, 1129, 1463, 1710, 2085, 2251
LweI	GCATC	8 cut(s)	136, 154, 312, 463, 619, 859, 1311, 2504
MaeI	CTAG	4 cut(s)	485, 695, 2102, 2405
MaeII	ACGT	3 cut(s)	562, 661, 1654
MaeIII	GTNAC	7 cut(s)	342, 446, 1076, 1477, 1570, 1894, 2728
MalI	GATC	7 cut(s)	258, 365, 671, 1500, 1878, 2228, 2373
MboI	GATC	7 cut(s)	256, 363, 669, 1498, 1876, 2226, 2371
MflI	RGATCY	1 cut(s)	1876
MhlI	GDGCHC	2 cut(s)	993, 1020
MlsI	TGGCCA	1 cut(s)	1185
MluNI	TGGCCA	1 cut(s)	1185
MlyI	GAGTC	3 cut(s)	387, 1016, 2659
MmeI	TCCRAC	3 cut(s)	435, 1951, 2816
Mox20I	TGGCCA	1 cut(s)	1185
Mph1103I	ATGCAT	3 cut(s)	151, 2197, 2519
MroNI	GCCGGC	2 cut(s)	1712, 2459
MroXI	GAANNNNTTC	5 cut(s)	222, 334, 650, 1034, 1980
MscI	TGGCCA	1 cut(s)	1185
MslI	CAYNNNNRTG	8 cut(s)	213, 258, 468, 858, 2057, 2522, 2682, 2688
Msp20I	TGGCCA	1 cut(s)	1185
MspA1I	CMGCKG	2 cut(s)	787, 1466
MspCI	CTTAAG	2 cut(s)	1880, 2630
MspI	CCGG	3 cut(s)	1713, 2460, 2566
MspR9I	CCNGG	4 cut(s)	361, 611, 1822, 1865
Mva1269I	GAATGC	2 cut(s)	1885, 2381
MvaI	CCWGG	4 cut(s)	361, 611, 1822, 1865
MwoI	GCNNNNNNNGC	8 cut(s)	11, 34, 523, 941, 1121, 1336, 1553, 1718
NaeI	GCCGGC	2 cut(s)	1714, 2461
NdeI	CATATG	3 cut(s)	151, 1332, 1993
NdeII	GATC	7 cut(s)	256, 363, 669, 1498, 1876, 2226, 2371
NgoMIV	GCCGGC	2 cut(s)	1712, 2459
NlaIV	GGNNCC	2 cut(s)	990, 2458
NmuCI	GTSAC	2 cut(s)	1477, 2728
NsbI	TGCGCA	1 cut(s)	2709
NsiI	ATGCAT	3 cut(s)	151, 2197, 2519
NspI	RCATGY	1 cut(s)	2714
NspV	TTCGAA	1 cut(s)	51
OliI	CACNNNNGTG	1 cut(s)	213
PaeR7I	CTCGAG	1 cut(s)	1890
PagI	TCATGA	1 cut(s)	2814
PciSI	GCTCTTC	1 cut(s)	2813
PctI	GAATGC	2 cut(s)	1885, 2381
PdiI	GCCGGC	2 cut(s)	1714, 2461
PdmI	GAANNNNTTC	5 cut(s)	222, 334, 650, 1034, 1980
PfeI	GAWTC	3 cut(s)	962, 1444, 1783
PfoI	TCCNGGA	1 cut(s)	359
PkrI	GCNGC	8 cut(s)	302, 634, 908, 1144, 1453, 1725, 2075, 2241
PleI	GAGTC	3 cut(s)	387, 1015, 2659
PpsI	GAGTC	3 cut(s)	387, 1015, 2659
PpuMI	RGGWCCY	1 cut(s)	2005
Psp124BI	GAGCTC	1 cut(s)	1020
Psp5II	RGGWCCY	1 cut(s)	2005
Psp6I	CCWGG	4 cut(s)	359, 609, 1820, 1863
PspEI	GGTNACC	1 cut(s)	446
PspFI	CCCAGC	2 cut(s)	1708, 2092
PspGI	CCWGG	4 cut(s)	359, 609, 1820, 1863
PspN4I	GGNNCC	2 cut(s)	990, 2458
PspPI	GGNCC	3 cut(s)	460, 1315, 2005
PspPPI	RGGWCCY	1 cut(s)	2005
PstI	CTGCAG	1 cut(s)	1227
PstNI	CAGNNNCTG	4 cut(s)	965, 1679, 1864, 2245
PsuI	RGATCY	1 cut(s)	1876
PvuII	CAGCTG	2 cut(s)	787, 1466
RsaI	GTAC	6 cut(s)	778, 1242, 1295, 1750, 2279, 2409
RsaNI	GTAC	6 cut(s)	777, 1241, 1294, 1749, 2278, 2408
RseI	CAYNNNNRTG	8 cut(s)	213, 258, 468, 858, 2057, 2522, 2682, 2688
SacI	GAGCTC	1 cut(s)	1020
SapI	GCTCTTC	1 cut(s)	2813
SatI	GCNGC	8 cut(s)	301, 633, 907, 1143, 1452, 1724, 2074, 2240
Sau3AI	GATC	7 cut(s)	256, 363, 669, 1498, 1876, 2226, 2371
Sau96I	GGNCC	3 cut(s)	460, 1315, 2005
ScaI	AGTACT	1 cut(s)	1242
SchI	GAGTC	3 cut(s)	387, 1016, 2659
ScrFI	CCNGG	4 cut(s)	361, 611, 1822, 1865
SduI	GDGCHC	2 cut(s)	993, 1020
SfaNI	GCATC	8 cut(s)	136, 154, 312, 463, 619, 859, 1311, 2504
SfcI	CTRYAG	2 cut(s)	812, 1223
Sfr274I	CTCGAG	1 cut(s)	1890
SfuI	TTCGAA	1 cut(s)	51
SinI	GGWCC	3 cut(s)	460, 1315, 2005
SlaI	CTCGAG	1 cut(s)	1890
SmiMI	CAYNNNNRTG	8 cut(s)	213, 258, 468, 858, 2057, 2522, 2682, 2688
SmlI	CTYRAG	9 cut(s)	427, 1810, 1880, 1890, 2414, 2477, 2630, 2737, 2776
SmoI	CTYRAG	9 cut(s)	427, 1810, 1880, 1890, 2414, 2477, 2630, 2737, 2776
SpeI	ACTAGT	1 cut(s)	2101
SsiI	CCGC	4 cut(s)	5, 167, 397, 2463
SspI	AATATT	1 cut(s)	898
SspMI	CTAG	4 cut(s)	485, 695, 2102, 2405
SstI	GAGCTC	1 cut(s)	1020
StyD4I	CCNGG	4 cut(s)	359, 609, 1820, 1863
StyI	CCWWGG	2 cut(s)	1039, 2065
TaaI	ACNGT	2 cut(s)	1190, 1675
TaiI	ACGT	3 cut(s)	565, 664, 1657
TaqI	TCGA	9 cut(s)	51, 322, 328, 466, 928, 1345, 1891, 2493, 2512
TatI	WGTACW	4 cut(s)	1240, 1748, 2277, 2407
TfiI	GAWTC	3 cut(s)	962, 1444, 1783
TscAI	CASTG	6 cut(s)	891, 1008, 1620, 2217, 2315, 2586
TseFI	GTSAC	2 cut(s)	1477, 2728
TseI	GCWGC	8 cut(s)	300, 632, 906, 1142, 1451, 1723, 2073, 2239
Tsp45I	GTSAC	2 cut(s)	1477, 2728
TspDTI	ATGAA	7 cut(s)	246, 1357, 1437, 2041, 2154, 2520, 2831
TspGWI	ACGGA	2 cut(s)	1496, 1580
TspRI	CASTG	6 cut(s)	891, 1008, 1620, 2217, 2315, 2586
Vha464I	CTTAAG	2 cut(s)	1880, 2630
VpaK11BI	GGWCC	3 cut(s)	460, 1315, 2005
XagI	CCTNNNNNAGG	1 cut(s)	455
XapI	RAATTY	6 cut(s)	90, 233, 404, 1045, 1484, 1914
XbaI	TCTAGA	1 cut(s)	484
XceI	RCATGY	1 cut(s)	2714
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1008, 2209
XhoI	CTCGAG	1 cut(s)	1890
XmiI	GTMKAC	1 cut(s)	624
XmnI	GAANNNNTTC	5 cut(s)	222, 334, 650, 1034, 1980
XspI	CTAG	4 cut(s)	485, 695, 2102, 2405
ZrmI	AGTACT	1 cut(s)	1242
Zsp2I	ATGCAT	3 cut(s)	151, 2197, 2519

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.