AT5G12470

Protein RETICULATA-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002218
Physical Location & Seq
Reverse (-)
4044620 .. 4047448
2829 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G12470.1

Sequence Viewer

Length: 1161 bp
ATGGCAATCGCTTCCTGCTTCTTCTGTGTTCCAACACCGAACACAGCTATCTCTGAATCCAATCTGACATGGCCTCACATCGCTTCGTTTCCTAGACTCTCTTCATCTTCCTCTTTTAACGGTGTAATCTCTGCAAAGTCAATCTCTTTTAACCGCCGTGTACCTATTACCCCTGTTTTATCCGCTTCAAGTGGTAACGGCGGCTCTGATAACAACGGAGGAGGTCTTAGTGGTGGTGGTGGTGGTGGCGATGGAGGTAAGAATGATGGAGATGGACACGGTGACGAGGACAGGGATAGGAACAGGAACGAGGCGATGCTTTTGTTGAAGGAGTCTGGGATTGAATTGGAGAGTCTTCCCAAAGATCTAGCGGCTGCTATTGAGGCGGGTCGGATTCCTGGATCGGTTATCACTCGGTTCCTGGAGCTTCAGAAATCGGCTGTGATGCGGTGGCTGATGCAGTTTGGTGGGTTTAGGGAAAGATTGTTGGCTGATGATCTCTTCATGGCTAAACTCGCCATGGAATGTGGTGTTGGAATCTTCACCAAGACTGCTGCTGAGTATGAACGGCGTAGAGAAAACTTCTTCAATGAACTTGAAGTTGTCTTTGCCGATGTGGCGATGGCTATTATTGCTGATTTCATGTTGGTTTATCTTCCTGCTCCTACCGTTTCTCTTCGACCACCTCTCGCACTTACTGCTGGAGGTATATCCAAGTTTTTCCACAACTGCCCAGACAATGCCTTCCAGGTTGCTCTCTCCGGAACCTCATATACTCTCTTGCAAAGGCTAGGTGCTATAACGCGCAATGGAGCTAAGCTGTTTGCCGTTGGCACCACATCATCGCTGGTTGGTACTGCTATTACAAATGCATTCATAAAAGCACGAAAAGCTGTGGACCAGAATTCTGAAGGTGAAGTCGAAACTGTTCCGATAGTCTCCACAAGTGTTGCATATGGTGTCTACATGGCAGTTTCTAGCAACCTAAGGTACCAAATAGTGGCTGGTGTGATTGAACAACGTCTCCTGGAGCCAATGCTACACCAACACAAACTTGCACTCAGTGCGTTGTGCTTTGCAGTTAGAACAGGCAACACATTCTTGGGTTCCTTATTATGGGTGGATTATGCCCGCCTCATTGGTATCCAGAAATCTCACTGA

Protein Analysis

386

Amino Acids

41.32

Weight (kDa)

7.01

Isoelectric Point (pI)

34.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RETICULATA-like PF11891 160 - 337 4.2e-66 Protein RETICULATA-related
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 990
AccB1I GGYRCC 2 cut(s) 833, 990
AccB7I CCANNNNNTGG 1 cut(s) 1000
AccI GTMKAC 1 cut(s) 963
AccII CGCG 1 cut(s) 805
AccIII TCCGGA 1 cut(s) 761
AciI CCGC 7 cut(s) 154, 183, 201, 371, 386, 448, 1132
AclWI GGATC 1 cut(s) 409
AcsI RAATTY 1 cut(s) 904
AcuI CTGAAG 2 cut(s) 413, 930
AdeI CACNNNGTG 1 cut(s) 1064
AfaI GTAC 3 cut(s) 162, 856, 992
AfiI CCNNNNNNNGG 2 cut(s) 1000, 1116
AgsI TTSAA 6 cut(s) 189, 328, 344, 589, 599, 1016
AjnI CCWGG 4 cut(s) 397, 420, 747, 1026
AjuI GAANNNNNNNTTGG 2 cut(s) 516, 548
AloI GAACNNNNNNTCC 2 cut(s) 1008, 1040
AluBI AGCT 5 cut(s) 47, 427, 815, 820, 893
AluI AGCT 5 cut(s) 47, 427, 815, 820, 893
Alw26I GTCTC 2 cut(s) 943, 1028
AlwI GGATC 1 cut(s) 409
Aor13HI TCCGGA 1 cut(s) 761
AoxI GGCC 1 cut(s) 71
ApeKI GCWGC 2 cut(s) 374, 554
ApoI RAATTY 1 cut(s) 904
Asp700I GAANNNNTTC 1 cut(s) 927
Asp718I GGTACC 1 cut(s) 990
AspLEI GCGC 1 cut(s) 807
AspS9I GGNCC 1 cut(s) 898
AsuHPI GGTGA 3 cut(s) 293, 535, 926
AvaII GGWCC 1 cut(s) 898
AxyI CCTNAGG 1 cut(s) 986
BanI GGYRCC 2 cut(s) 833, 990
BbsI GAAGAC 1 cut(s) 347
BbvI GCAGC 2 cut(s) 361, 541
BccI CCATC 4 cut(s) 245, 260, 266, 616
BceAI ACGGC 4 cut(s) 141, 214, 584, 812
BciT130I CCWGG 4 cut(s) 399, 422, 749, 1028
BciVI GTATCC 1 cut(s) 1154
BcoDI GTCTC 2 cut(s) 943, 1028
BfaI CTAG 4 cut(s) 93, 368, 791, 978
BfuI GTATCC 1 cut(s) 1154
BglI GCCNNNNNGGC 1 cut(s) 617
BglII AGATCT 1 cut(s) 364
BisI GCNGC 4 cut(s) 202, 372, 375, 555
BlpI GCTNAGC 1 cut(s) 816
BlsI GCNGC 4 cut(s) 203, 373, 376, 556
Bme1390I CCNGG 4 cut(s) 399, 422, 749, 1028
Bme18I GGWCC 1 cut(s) 898
BmgT120I GGNCC 1 cut(s) 898
BmiI GGNNCC 6 cut(s) 419, 766, 835, 992, 1032, 1108
BmrFI CCNGG 4 cut(s) 399, 422, 749, 1028
BmsI GCATC 3 cut(s) 306, 435, 447
BpiI GAAGAC 1 cut(s) 347
BpmI CTGGAG 3 cut(s) 443, 723, 1049
Bpu1102I GCTNAGC 1 cut(s) 816
BsaJI CCNNGG 1 cut(s) 519
BsaWI WCCGGW 1 cut(s) 761
BsaXI ACNNNNNCTCC 2 cut(s) 1008, 1038
Bsc4I CCNNNNNNNGG 2 cut(s) 1000, 1116
Bse21I CCTNAGG 1 cut(s) 986
Bse3DI GCAATG 1 cut(s) 814
BseAI TCCGGA 1 cut(s) 761
BseBI CCWGG 4 cut(s) 399, 422, 749, 1028
BseDI CCNNGG 1 cut(s) 519
BseLI CCNNNNNNNGG 2 cut(s) 1000, 1116
BseMI GCAATG 1 cut(s) 814
BseMII CTCAG 2 cut(s) 549, 1075
BseRI GAGGAG 1 cut(s) 234
BseXI GCAGC 2 cut(s) 361, 541
Bsh1236I CGCG 1 cut(s) 805
BshFI GGCC 1 cut(s) 73
BshNI GGYRCC 2 cut(s) 833, 990
BsiSI CCGG 1 cut(s) 762
BslI CCNNNNNNNGG 2 cut(s) 1000, 1116
BsmAI GTCTC 2 cut(s) 943, 1028
BsmBI CGTCTC 1 cut(s) 1028
BsmI GAATGC 1 cut(s) 872
BsnI GGCC 1 cut(s) 73
Bsp13I TCCGGA 1 cut(s) 761
Bsp143I GATC 3 cut(s) 364, 401, 496
Bsp1720I GCTNAGC 1 cut(s) 816
Bsp19I CCATGG 1 cut(s) 519
BspACI CCGC 7 cut(s) 154, 183, 201, 371, 386, 448, 1132
BspANI GGCC 1 cut(s) 73
BspCNI CTCAG 2 cut(s) 550, 1074
BspEI TCCGGA 1 cut(s) 761
BspFNI CGCG 1 cut(s) 805
BspLI GGNNCC 6 cut(s) 419, 766, 835, 992, 1032, 1108
BspPI GGATC 1 cut(s) 409
BspT107I GGYRCC 2 cut(s) 833, 990
BsrDI GCAATG 1 cut(s) 814
BssECI CCNNGG 1 cut(s) 519
BssMI GATC 3 cut(s) 364, 401, 496
BssT1I CCWWGG 1 cut(s) 519
Bst2UI CCWGG 4 cut(s) 399, 422, 749, 1028
Bst4CI ACNGT 4 cut(s) 122, 281, 670, 928
Bst6I CTCTTC 3 cut(s) 106, 506, 681
BstAPI GCANNNNNTGC 2 cut(s) 698, 1064
BstC8I GCNNGC 1 cut(s) 1132
BstDEI CTNAG 5 cut(s) 227, 558, 816, 986, 1061
BstDSI CCRYGG 1 cut(s) 519
BstFNI CGCG 1 cut(s) 805
BstHHI GCGC 1 cut(s) 807
BstKTI GATC 3 cut(s) 367, 404, 499
BstMAI GTCTC 2 cut(s) 943, 1028
BstMBI GATC 3 cut(s) 364, 401, 496
BstMWI GCNNNNNNNGC 7 cut(s) 383, 515, 617, 632, 698, 890, 1064
BstNI CCWGG 4 cut(s) 399, 422, 749, 1028
BstSCI CCNGG 4 cut(s) 397, 420, 747, 1026
BstUI CGCG 1 cut(s) 805
BstV1I GCAGC 2 cut(s) 361, 541
BstV2I GAAGAC 1 cut(s) 347
BstX2I RGATCY 1 cut(s) 364
BstYI RGATCY 1 cut(s) 364
Bsu36I CCTNAGG 1 cut(s) 986
BsuI GTATCC 1 cut(s) 1154
BsuRI GGCC 1 cut(s) 73
BtgI CCRYGG 1 cut(s) 519
BtgZI GCGATG 5 cut(s) 64, 264, 329, 635, 828
BtsIMutI CAGTG 2 cut(s) 1069, 1156
Cac8I GCNNGC 1 cut(s) 1132
CfoI GCGC 1 cut(s) 807
Cfr13I GGNCC 1 cut(s) 898
Csp6I GTAC 3 cut(s) 161, 855, 991
CspCI CAANNNNNGTGG 2 cut(s) 931, 966
CviAII CATG 5 cut(s) 69, 505, 520, 643, 967
CviQI GTAC 3 cut(s) 161, 855, 991
DdeI CTNAG 5 cut(s) 227, 558, 816, 986, 1061
DpnI GATC 3 cut(s) 366, 403, 498
DpnII GATC 3 cut(s) 364, 401, 496
DraIII CACNNNGTG 1 cut(s) 1064
Eam1104I CTCTTC 3 cut(s) 106, 506, 681
EarI CTCTTC 3 cut(s) 106, 506, 681
Eco130I CCWWGG 1 cut(s) 519
Eco47I GGWCC 1 cut(s) 898
Eco57I CTGAAG 2 cut(s) 413, 930
Eco81I CCTNAGG 1 cut(s) 986
EcoRI GAATTC 1 cut(s) 904
EcoRII CCWGG 4 cut(s) 397, 420, 747, 1026
EcoT14I CCWWGG 1 cut(s) 519
EcoT22I ATGCAT 1 cut(s) 874
ErhI CCWWGG 1 cut(s) 519
Esp3I CGTCTC 1 cut(s) 1028
FaeI CATG 5 cut(s) 72, 508, 523, 646, 970
FatI CATG 5 cut(s) 68, 504, 519, 642, 966
FauI CCCGC 2 cut(s) 379, 1139
FauNDI CATATG 1 cut(s) 955
FblI GTMKAC 1 cut(s) 963
Fnu4HI GCNGC 4 cut(s) 202, 372, 375, 555
Fsp4HI GCNGC 4 cut(s) 202, 372, 375, 555
FspBI CTAG 4 cut(s) 93, 368, 791, 978
GlaI GCGC 1 cut(s) 806
GluI GCNGC 4 cut(s) 202, 372, 375, 555
GsuI CTGGAG 3 cut(s) 443, 723, 1049
HaeIII GGCC 1 cut(s) 73
HapII CCGG 1 cut(s) 762
HhaI GCGC 1 cut(s) 807
Hin1II CATG 5 cut(s) 72, 508, 523, 646, 970
Hin6I GCGC 1 cut(s) 805
HinP1I GCGC 1 cut(s) 805
HinfI GANTC 6 cut(s) 56, 96, 332, 352, 394, 537
HpaII CCGG 1 cut(s) 762
HphI GGTGA 3 cut(s) 293, 535, 926
Hpy166II GTNNAC 3 cut(s) 161, 898, 964
Hpy188I TCNGA 7 cut(s) 55, 66, 208, 393, 432, 910, 933
Hpy188III TCNNGA 2 cut(s) 762, 1147
Hpy8I GTNNAC 3 cut(s) 161, 898, 964
HpyAV CCTTC 3 cut(s) 322, 754, 905
HpyCH4III ACNGT 4 cut(s) 122, 281, 670, 928
HpyCH4IV ACGT 1 cut(s) 1021
HpyCH4V TGCA 7 cut(s) 134, 460, 784, 872, 953, 1058, 1079
HpyF10VI GCNNNNNNNGC 7 cut(s) 383, 515, 617, 632, 698, 890, 1064
HpyF3I CTNAG 5 cut(s) 227, 558, 816, 986, 1061
HpySE526I ACGT 1 cut(s) 1021
Hsp92II CATG 5 cut(s) 72, 508, 523, 646, 970
HspAI GCGC 1 cut(s) 805
Kpn2I TCCGGA 1 cut(s) 761
KpnI GGTACC 1 cut(s) 994
Kzo9I GATC 3 cut(s) 364, 401, 496
LmnI GCTCC 4 cut(s) 424, 667, 812, 1030
Lsp1109I GCAGC 2 cut(s) 361, 541
LweI GCATC 3 cut(s) 306, 435, 447
MaeI CTAG 4 cut(s) 93, 368, 791, 978
MaeII ACGT 1 cut(s) 1021
MaeIII GTNAC 2 cut(s) 194, 281
MalI GATC 3 cut(s) 366, 403, 498
MboI GATC 3 cut(s) 364, 401, 496
MboII GAAGA 9 cut(s) 13, 93, 99, 347, 493, 532, 577, 647, 668
MflI RGATCY 1 cut(s) 364
MluCI AATT 2 cut(s) 344, 904
MlyI GAGTC 3 cut(s) 90, 341, 361
MmeI TCCRAC 3 cut(s) 56, 371, 514
Mph1103I ATGCAT 1 cut(s) 874
MroI TCCGGA 1 cut(s) 761
MroXI GAANNNNTTC 1 cut(s) 927
MseI TTAA 2 cut(s) 117, 150
MspI CCGG 1 cut(s) 762
MspR9I CCNGG 4 cut(s) 399, 422, 749, 1028
Mva1269I GAATGC 1 cut(s) 872
MvaI CCWGG 4 cut(s) 399, 422, 749, 1028
MvnI CGCG 1 cut(s) 805
MwoI GCNNNNNNNGC 7 cut(s) 383, 515, 617, 632, 698, 890, 1064
NcoI CCATGG 1 cut(s) 519
NdeI CATATG 1 cut(s) 955
NdeII GATC 3 cut(s) 364, 401, 496
NlaIII CATG 5 cut(s) 72, 508, 523, 646, 970
NlaIV GGNNCC 6 cut(s) 419, 766, 835, 992, 1032, 1108
NmuCI GTSAC 1 cut(s) 281
NsiI ATGCAT 1 cut(s) 874
PctI GAATGC 1 cut(s) 872
PdmI GAANNNNTTC 1 cut(s) 927
PfeI GAWTC 3 cut(s) 56, 394, 537
PflMI CCANNNNNTGG 1 cut(s) 1000
PfoI TCCNGGA 3 cut(s) 397, 420, 1026
PkrI GCNGC 4 cut(s) 203, 373, 376, 556
PleI GAGTC 3 cut(s) 90, 340, 360
PpsI GAGTC 3 cut(s) 90, 340, 360
Psp6I CCWGG 4 cut(s) 397, 420, 747, 1026
PspGI CCWGG 4 cut(s) 397, 420, 747, 1026
PspN4I GGNNCC 6 cut(s) 419, 766, 835, 992, 1032, 1108
PspPI GGNCC 1 cut(s) 898
PsrI GAACNNNNNNTAC 2 cut(s) 757, 789
PsuI RGATCY 1 cut(s) 364
RsaI GTAC 3 cut(s) 162, 856, 992
RsaNI GTAC 3 cut(s) 161, 855, 991
SaqAI TTAA 2 cut(s) 117, 150
SatI GCNGC 4 cut(s) 202, 372, 375, 555
Sau3AI GATC 3 cut(s) 364, 401, 496
Sau96I GGNCC 1 cut(s) 898
SchI GAGTC 3 cut(s) 90, 341, 361
ScrFI CCNGG 4 cut(s) 399, 422, 749, 1028
SfaNI GCATC 3 cut(s) 306, 435, 447
SinI GGWCC 1 cut(s) 898
Sse9I AATT 2 cut(s) 344, 904
SsiI CCGC 7 cut(s) 154, 183, 201, 371, 386, 448, 1132
SspMI CTAG 4 cut(s) 93, 368, 791, 978
StyD4I CCNGG 4 cut(s) 397, 420, 747, 1026
StyI CCWWGG 1 cut(s) 519
TaaI ACNGT 4 cut(s) 122, 281, 670, 928
TaiI ACGT 1 cut(s) 1024
TaqI TCGA 2 cut(s) 679, 921
TasI AATT 2 cut(s) 344, 904
TauI GCSGC 2 cut(s) 204, 374
TfiI GAWTC 3 cut(s) 56, 394, 537
Tru1I TTAA 2 cut(s) 117, 150
Tru9I TTAA 2 cut(s) 117, 150
TscAI CASTG 1 cut(s) 1069
TseFI GTSAC 1 cut(s) 281
TseI GCWGC 2 cut(s) 374, 554
Tsp45I GTSAC 1 cut(s) 281
TspDTI ATGAA 6 cut(s) 93, 493, 579, 606, 631, 865
TspGWI ACGGA 1 cut(s) 231
TspRI CASTG 1 cut(s) 1069
Van91I CCANNNNNTGG 1 cut(s) 1000
VpaK11BI GGWCC 1 cut(s) 898
XapI RAATTY 1 cut(s) 904
XcmI CCANNNNNNNNNTGG 2 cut(s) 844, 1001
XmiI GTMKAC 1 cut(s) 963
XmnI GAANNNNTTC 1 cut(s) 927
XspI CTAG 4 cut(s) 93, 368, 791, 978
Zsp2I ATGCAT 1 cut(s) 874
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.