pycom02g25410

Protein RETICULATA-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0002218
Physical Location & Seq
Reverse (-)
23349093 .. 23353064
3972 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g25410.8

Sequence Viewer

Length: 1155 bp
ATGGCTACAGTCACCACCATCTCCAACTTCTACACTTCTCACTCTCAATTTCCCCCTTCACCAAACACCCTCCGCCATTTCTCTCTCTCTCCAAAAAGCTTCACTTTCACCATCATCTCGCGCACTAACATCACCGCAACTCCCTCCCTCCAACTCACCACCTCCCGCCTCAACCGATCCCCGCTCGCCGCCGCGGCATACGGCGGTGAGCCCCCATCAGGCGGAGGTGGACACGGCGGAAGTGGAGGTAATAACAATGACGGTGATGGAAGTGGTGAGGACCGAGATCGAAACAAGGCGGAGGCCCTGGCGGCAATGGCTGAGGCTGGGAGGTCGTCGGAGAGTCTGCCGAAGGACTTGGCGGCGGCCATAGAGGCGGGGCGGGTGCCGGGGTTGATTGTGAAGAGGTTCTTTGAGCTGGAGAAGTCGGCGGTGTTCAGGTGGTTGCTTGGGTTTGATGGGTTCAAGGAGCGGTTGCTTGCGGACGACTTGTTCTTGACTAAAGTTGGCATCGAGTGCGGCGTTGGGATATTCACCAAGACTGCTGCAGAATTGGAGCGGCGCCGAGAAAATTTTACCAAAGAGCTGGATTTTGTTTTTGCGGATGTGGTGATGGCCATCATAGCTGATTTTATGCTTGTCTGGCTTCCCGCTCCTACTGTTTCACTTCGACCACCTCTTGCTGTCAGCGCAGGCCGTATTGCTAAGTTCTTCTATGGGTGTCCGGATAATGCTTTCCAGGTAGCTTTGTCTGGAACATCTTACTCACTCTTACAGAGAATAGGCGCAATAGTGAGAAATGGAGCTAAACTATTTGCAGTCGGGACTGGTGCATCTCTGGTTGGTACGGGTGTAACCAATGCATTGATTAATGCACGAAAAGCTCTCGATAAATCTTTTGCTGGTGAAGCAGAGGATGTTCCCATATTATCAACTAGTGTAGCCTATGGAGTTTATATGGCGGTTTCTAGCAATCTAAGGTACCAAGTTCTTGCCGGAGTAATTGAACAACGCCTTCTAGAACCCTTGCTGCACCAACACAAGCTTATACTGAGTGCAGTGTGCTTTGCTGTTCGAACTGGCAACACGTTCTTGGGTTCCTTGATGTGGGTCGATTATGCACGTTGGATTGGAATTCAAAAGATCCGAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RETICULATA-like

Protein Analysis

385

Amino Acids

41.26

Weight (kDa)

9.06

Isoelectric Point (pI)

42.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 981
AccB1I GGYRCC 3 cut(s) 385, 561, 981
AccBSI CCGCTC 4 cut(s) 184, 472, 559, 653
AccII CGCG 2 cut(s) 121, 194
AccIII TCCGGA 1 cut(s) 724
AclWI GGATC 2 cut(s) 171, 1138
AcoI YGGCCR 2 cut(s) 366, 615
AcsI RAATTY 2 cut(s) 571, 1134
AcyI GRCGYC 1 cut(s) 562
AfaI GTAC 2 cut(s) 847, 983
AfiI CCNNNNNNNGG 3 cut(s) 218, 221, 1107
AflIII ACRYGT 1 cut(s) 1086
AgsI TTSAA 3 cut(s) 466, 1007, 1139
AhlI ACTAGT 1 cut(s) 935
AjnI CCWGG 2 cut(s) 306, 738
AloI GAACNNNNNNTCC 2 cut(s) 476, 508
AluBI AGCT 8 cut(s) 99, 418, 586, 626, 746, 806, 884, 1045
AluI AGCT 8 cut(s) 99, 418, 586, 626, 746, 806, 884, 1045
AlwI GGATC 2 cut(s) 171, 1138
Aor13HI TCCGGA 1 cut(s) 724
AoxI GGCC 4 cut(s) 303, 366, 615, 694
ApeKI GCWGC 2 cut(s) 545, 1030
ApoI RAATTY 2 cut(s) 571, 1134
AseI ATTAAT 1 cut(s) 870
Asp700I GAANNNNTTC 1 cut(s) 407
Asp718I GGTACC 1 cut(s) 981
AspLEI GCGC 4 cut(s) 123, 564, 692, 788
AspS9I GGNCC 2 cut(s) 280, 304
AsuC2I CCSGG 1 cut(s) 390
AsuII TTCGAA 1 cut(s) 1075
AvaII GGWCC 1 cut(s) 280
BaeI ACNNNNGTAYC 2 cut(s) 837, 870
BalI TGGCCA 1 cut(s) 617
BanI GGYRCC 3 cut(s) 385, 561, 981
BanII GRGCYC 1 cut(s) 213
BbvCI CCTCAGC 1 cut(s) 321
BbvI GCAGC 2 cut(s) 532, 1017
BccI CCATC 7 cut(s) 26, 119, 223, 260, 452, 607, 626
BceAI ACGGC 3 cut(s) 217, 250, 681
BciT130I CCWGG 2 cut(s) 308, 740
BcnI CCSGG 1 cut(s) 390
BcuI ACTAGT 1 cut(s) 935
BfaI CTAG 3 cut(s) 936, 969, 1019
BfmI CTRYAG 2 cut(s) 6, 546
BfoI RGCGCY 1 cut(s) 565
BglI GCCNNNNNGGC 3 cut(s) 194, 311, 374
Bme1390I CCNGG 3 cut(s) 308, 390, 740
Bme18I GGWCC 1 cut(s) 280
BmgT120I GGNCC 2 cut(s) 280, 304
BmiI GGNNCC 4 cut(s) 387, 563, 983, 1099
BmrFI CCNGG 3 cut(s) 308, 390, 740
BmsI GCATC 2 cut(s) 519, 842
BpmI CTGGAG 1 cut(s) 440
Bpu10I CCTNAGC 1 cut(s) 321
Bpu14I TTCGAA 1 cut(s) 1075
BpuMI CCSGG 1 cut(s) 390
BsaBI GATNNNNATC 1 cut(s) 617
BsaHI GRCGYC 1 cut(s) 562
BsaJI CCNNGG 3 cut(s) 192, 306, 389
BsaWI WCCGGW 1 cut(s) 724
BsaXI ACNNNNNCTCC 2 cut(s) 124, 154
Bsc4I CCNNNNNNNGG 3 cut(s) 218, 221, 1107
Bse1I ACTGG 2 cut(s) 832, 1084
Bse3DI GCAATG 1 cut(s) 321
Bse8I GATNNNNATC 1 cut(s) 617
BseAI TCCGGA 1 cut(s) 724
BseBI CCWGG 2 cut(s) 308, 740
BseDI CCNNGG 3 cut(s) 192, 306, 389
BseGI GGATG 2 cut(s) 610, 922
BseJI GATNNNNATC 1 cut(s) 617
BseLI CCNNNNNNNGG 3 cut(s) 218, 221, 1107
BseMI GCAATG 1 cut(s) 321
BseMII CTCAG 2 cut(s) 312, 1043
BseNI ACTGG 2 cut(s) 832, 1084
BseXI GCAGC 2 cut(s) 532, 1017
BseYI CCCAGC 1 cut(s) 326
BsgI GTGCAG 2 cut(s) 1016, 1077
Bsh1236I CGCG 2 cut(s) 121, 194
BshFI GGCC 4 cut(s) 305, 368, 617, 696
BshNI GGYRCC 3 cut(s) 385, 561, 981
BsiSI CCGG 3 cut(s) 389, 725, 996
BslFI GGGAC 1 cut(s) 838
BslI CCNNNNNNNGG 3 cut(s) 218, 221, 1107
BsmFI GGGAC 1 cut(s) 838
BsnI GGCC 4 cut(s) 305, 368, 617, 696
Bsp119I TTCGAA 1 cut(s) 1075
Bsp1286I GDGCHC 1 cut(s) 213
Bsp13I TCCGGA 1 cut(s) 724
Bsp143I GATC 3 cut(s) 176, 286, 1143
BspANI GGCC 4 cut(s) 305, 368, 617, 696
BspCNI CTCAG 2 cut(s) 313, 1044
BspEI TCCGGA 1 cut(s) 724
BspFNI CGCG 2 cut(s) 121, 194
BspLI GGNNCC 4 cut(s) 387, 563, 983, 1099
BspMAI CTGCAG 1 cut(s) 550
BspPI GGATC 2 cut(s) 171, 1138
BspT104I TTCGAA 1 cut(s) 1075
BspT107I GGYRCC 3 cut(s) 385, 561, 981
BsrBI CCGCTC 4 cut(s) 184, 472, 559, 653
BsrDI GCAATG 1 cut(s) 321
BsrI ACTGG 2 cut(s) 832, 1084
BssECI CCNNGG 3 cut(s) 192, 306, 389
BssMI GATC 3 cut(s) 176, 286, 1143
BssNI GRCGYC 1 cut(s) 562
Bst2UI CCWGG 2 cut(s) 308, 740
Bst4CI ACNGT 3 cut(s) 10, 263, 661
Bst6I CTCTTC 1 cut(s) 398
BstACI GRCGYC 1 cut(s) 562
BstAPI GCANNNNNTGC 1 cut(s) 516
BstBI TTCGAA 1 cut(s) 1075
BstC8I GCNNGC 3 cut(s) 186, 480, 694
BstDEI CTNAG 4 cut(s) 321, 705, 977, 1052
BstDSI CCRYGG 1 cut(s) 192
BstF5I GGATG 2 cut(s) 610, 922
BstFNI CGCG 2 cut(s) 121, 194
BstH2I RGCGCY 1 cut(s) 565
BstHHI GCGC 4 cut(s) 123, 564, 692, 788
BstKTI GATC 3 cut(s) 179, 289, 1146
BstMBI GATC 3 cut(s) 176, 286, 1143
BstNI CCWGG 2 cut(s) 308, 740
BstSCI CCNGG 3 cut(s) 306, 388, 738
BstSFI CTRYAG 2 cut(s) 6, 546
BstUI CGCG 2 cut(s) 121, 194
BstV1I GCAGC 2 cut(s) 532, 1017
BstX2I RGATCY 1 cut(s) 1143
BstXI CCANNNNNNTGG 1 cut(s) 586
BstYI RGATCY 1 cut(s) 1143
BsuRI GGCC 4 cut(s) 305, 368, 617, 696
BtgI CCRYGG 1 cut(s) 192
BtsCI GGATG 2 cut(s) 610, 922
BtsI GCAGTG 1 cut(s) 1065
BtsIMutI CAGTG 1 cut(s) 1065
Cac8I GCNNGC 3 cut(s) 186, 480, 694
CfoI GCGC 4 cut(s) 123, 564, 692, 788
Cfr13I GGNCC 2 cut(s) 280, 304
Cfr42I CCGCGG 1 cut(s) 195
Csp6I GTAC 2 cut(s) 846, 982
CviQI GTAC 2 cut(s) 846, 982
DdeI CTNAG 4 cut(s) 321, 705, 977, 1052
DinI GGCGCC 1 cut(s) 563
DpnI GATC 3 cut(s) 178, 288, 1145
DpnII GATC 3 cut(s) 176, 286, 1143
EaeI YGGCCR 2 cut(s) 366, 615
Eam1104I CTCTTC 1 cut(s) 398
EarI CTCTTC 1 cut(s) 398
EciI GGCGGA 4 cut(s) 62, 237, 252, 314
Eco24I GRGCYC 1 cut(s) 213
Eco47I GGWCC 1 cut(s) 280
EcoO109I RGGNCCY 1 cut(s) 304
EcoRI GAATTC 1 cut(s) 1134
EcoRII CCWGG 2 cut(s) 306, 738
EcoT22I ATGCAT 1 cut(s) 865
EcoT38I GRGCYC 1 cut(s) 213
EgeI GGCGCC 1 cut(s) 563
EheI GGCGCC 1 cut(s) 563
FalI AAGNNNNNCTT 4 cut(s) 88, 120, 395, 427
FaqI GGGAC 1 cut(s) 838
FauI CCCGC 5 cut(s) 173, 189, 370, 375, 658
FokI GGATG 2 cut(s) 617, 929
FriOI GRGCYC 1 cut(s) 213
FspBI CTAG 3 cut(s) 936, 969, 1019
GlaI GCGC 4 cut(s) 122, 563, 691, 787
GsaI CCCAGC 1 cut(s) 330
GsuI CTGGAG 1 cut(s) 440
HaeII RGCGCY 1 cut(s) 565
HaeIII GGCC 4 cut(s) 305, 368, 617, 696
HapII CCGG 3 cut(s) 389, 725, 996
HhaI GCGC 4 cut(s) 123, 564, 692, 788
Hin1I GRCGYC 1 cut(s) 562
Hin6I GCGC 4 cut(s) 121, 562, 690, 786
HinP1I GCGC 4 cut(s) 121, 562, 690, 786
HindIII AAGCTT 2 cut(s) 97, 1043
HinfI GANTC 1 cut(s) 343
HpaII CCGG 3 cut(s) 389, 725, 996
Hpy166II GTNNAC 1 cut(s) 230
Hpy188I TCNGA 2 cut(s) 340, 1148
Hpy188III TCNNGA 6 cut(s) 496, 725, 753, 823, 887, 1019
Hpy8I GTNNAC 1 cut(s) 230
Hpy99I CGWCG 1 cut(s) 340
HpyAV CCTTC 3 cut(s) 66, 346, 1025
HpyCH4III ACNGT 3 cut(s) 10, 263, 661
HpyCH4IV ACGT 2 cut(s) 1088, 1123
HpyCH4V TGCA 8 cut(s) 548, 818, 833, 863, 875, 1033, 1058, 1121
HpyF3I CTNAG 4 cut(s) 321, 705, 977, 1052
HpySE526I ACGT 2 cut(s) 1088, 1123
Hsp92I GRCGYC 1 cut(s) 562
HspAI GCGC 4 cut(s) 121, 562, 690, 786
KasI GGCGCC 1 cut(s) 561
Kpn2I TCCGGA 1 cut(s) 724
KpnI GGTACC 1 cut(s) 985
KspI CCGCGG 1 cut(s) 195
Kzo9I GATC 3 cut(s) 176, 286, 1143
LmnI GCTCC 4 cut(s) 469, 556, 658, 803
Lsp1109I GCAGC 2 cut(s) 532, 1017
LweI GCATC 2 cut(s) 519, 842
MaeI CTAG 3 cut(s) 936, 969, 1019
MaeII ACGT 2 cut(s) 1088, 1123
MaeIII GTNAC 2 cut(s) 10, 853
MalI GATC 3 cut(s) 178, 288, 1145
MbiI CCGCTC 4 cut(s) 184, 472, 559, 653
MboI GATC 3 cut(s) 176, 286, 1143
MboII GAAGA 2 cut(s) 415, 703
MflI RGATCY 1 cut(s) 1143
MhlI GDGCHC 1 cut(s) 213
MlsI TGGCCA 1 cut(s) 617
MluCI AATT 5 cut(s) 47, 551, 571, 1002, 1134
MluNI TGGCCA 1 cut(s) 617
Mly113I GGCGCC 1 cut(s) 562
MlyI GAGTC 1 cut(s) 352
MmeI TCCRAC 4 cut(s) 48, 175, 318, 1106
Mox20I TGGCCA 1 cut(s) 617
Mph1103I ATGCAT 1 cut(s) 865
MroI TCCGGA 1 cut(s) 724
MroXI GAANNNNTTC 1 cut(s) 407
MscI TGGCCA 1 cut(s) 617
MseI TTAA 1 cut(s) 870
Msp20I TGGCCA 1 cut(s) 617
MspA1I CMGCKG 1 cut(s) 194
MspI CCGG 3 cut(s) 389, 725, 996
MspR9I CCNGG 3 cut(s) 308, 390, 740
MvaI CCWGG 2 cut(s) 308, 740
MvnI CGCG 2 cut(s) 121, 194
NarI GGCGCC 1 cut(s) 562
NciI CCSGG 1 cut(s) 390
NdeII GATC 3 cut(s) 176, 286, 1143
NlaIV GGNNCC 4 cut(s) 387, 563, 983, 1099
NmeAIII GCCGAG 1 cut(s) 590
NmuCI GTSAC 1 cut(s) 10
NsiI ATGCAT 1 cut(s) 865
NspV TTCGAA 1 cut(s) 1075
PcsI WCGNNNNNNNCGW 1 cut(s) 519
PdmI GAANNNNTTC 1 cut(s) 407
PleI GAGTC 1 cut(s) 351
PluTI GGCGCC 1 cut(s) 565
PpsI GAGTC 1 cut(s) 351
PshBI ATTAAT 1 cut(s) 870
Psp6I CCWGG 2 cut(s) 306, 738
PspFI CCCAGC 1 cut(s) 326
PspGI CCWGG 2 cut(s) 306, 738
PspN4I GGNNCC 4 cut(s) 387, 563, 983, 1099
PspPI GGNCC 2 cut(s) 280, 304
PstI CTGCAG 1 cut(s) 550
PsuI RGATCY 1 cut(s) 1143
RsaI GTAC 2 cut(s) 847, 983
RsaNI GTAC 2 cut(s) 846, 982
SacII CCGCGG 1 cut(s) 195
SaqAI TTAA 1 cut(s) 870
Sau3AI GATC 3 cut(s) 176, 286, 1143
Sau96I GGNCC 2 cut(s) 280, 304
SchI GAGTC 1 cut(s) 352
ScrFI CCNGG 3 cut(s) 308, 390, 740
SduI GDGCHC 1 cut(s) 213
SfaNI GCATC 2 cut(s) 519, 842
SfcI CTRYAG 2 cut(s) 6, 546
SfoI GGCGCC 1 cut(s) 563
Sfr303I CCGCGG 1 cut(s) 195
SfuI TTCGAA 1 cut(s) 1075
SgrBI CCGCGG 1 cut(s) 195
SinI GGWCC 1 cut(s) 280
SpeI ACTAGT 1 cut(s) 935
Sse9I AATT 5 cut(s) 47, 551, 571, 1002, 1134
SspDI GGCGCC 1 cut(s) 561
SspMI CTAG 3 cut(s) 936, 969, 1019
StyD4I CCNGG 3 cut(s) 306, 388, 738
TaaI ACNGT 3 cut(s) 10, 263, 661
TaiI ACGT 2 cut(s) 1091, 1126
TaqI TCGA 6 cut(s) 289, 513, 670, 888, 1075, 1113
TaqII GACCGA 1 cut(s) 297
TasI AATT 5 cut(s) 47, 551, 571, 1002, 1134
TauI GCSGC 8 cut(s) 191, 194, 197, 314, 365, 368, 522, 562
Tru1I TTAA 1 cut(s) 870
Tru9I TTAA 1 cut(s) 870
TscAI CASTG 1 cut(s) 1065
TseFI GTSAC 1 cut(s) 10
TseI GCWGC 2 cut(s) 545, 1030
Tsp45I GTSAC 1 cut(s) 10
TspRI CASTG 1 cut(s) 1065
VpaK11BI GGWCC 1 cut(s) 280
VspI ATTAAT 1 cut(s) 870
XapI RAATTY 2 cut(s) 571, 1134
XbaI TCTAGA 1 cut(s) 1018
XmnI GAANNNNTTC 1 cut(s) 407
XspI CTAG 3 cut(s) 936, 969, 1019
Zsp2I ATGCAT 1 cut(s) 865
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.