MD02G1301900.v1.1

Protein RETICULATA-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0002218
Physical Location & Seq
Reverse (-)
35623845 .. 35628167
4323 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1301900.v1.1.491

Sequence Viewer

Length: 1155 bp
ATGGCTGCAGTCACCACCATCTCCAACTTCTACACTTCTCACTCTCAATTTCCCCCTTCACCAAACACCCCCCGCCATTTCTCTCTCTCTCCAAAAAGCTTCACTCTCACCATCATCTCCCGCACTAACATCACCGCAACTCCCTCCCTCCAACTCACCACCTCCCGCCTCCACCGATCCCCCCTCGCCGCCGCCGCATACGGCGGTGAGCCCCCATCAGGCGGAGGTGGACACGGCGGAAGTGGAGGTAATAACAATGACGGTGATGGAAGTGGTGAGGACCGAGATCGAAACAAGGCGGAGGCCCTGGCGGCAATGGCTGAGGCTGGGAGGTCGTCGGAGAGTCTCCCGAAGGACTTGGCGGCGGCTATAGAGGCGGGGCGGGTGCCGGGGTTGATTGTGAAGAGGTTCTTTGAGCTGGAGAAGTCGGCGGTGTTCAGGTGGTTGCTTGGGTTTGATGGGTTCAAGGAGCGGTTGCTTGCGGACGACTTGTTCTTGACTAAAGTTGGCATCGAGTGCGGCGTTGGGATATTCACCAAGACTGCTGCAGAATTGGAGCGGCGCCGAGAAAATTTTACCAAAGAGCTCGATTTTGTTTTTGCGGATGTGGTGATGGCCATCATAGCTGATTTTATGCTTGTTTGGCTTCCCGCTCCTACTGTTTCACTTCGACCACCTCTTGCTGTCAGCGCAGGCCGTATTGCTAAGTTCTTCTATGGGTGTCCAGATAATGCTTTCCAGGTAGCTTTGTCTGGAACCTCTTACTCACTCTTACAGAGAATAGGCGCAATAGTGAGAAATGGAGCTAAACTATTTGCAGTCGGGACTGGTGCATCTCTGGTTGGTACGGGTGTAACCAATGCCTTGATTAATGCACGAAAAGCTATCGATAAATCTTTTGCTGGTGAAGCAGAGGATGTTCCCATATTGTCAACTAGTGTAGCTTATGGAGTTTATATGGCGGTTTCTAGCAATCTAAGGTACCAAGTTCTCGCCGGAGTAATTGAACAACGCCTTCTTGAACCCTTGCTGCACCAAAACAAGCTTATACTGAGTGCAATCTGCTTTGCTGTTCGAACTGGCAACACGTTCTTGGGTTCCTTGATGTGGGTCGATTATGCACGTTGGATTGGAATTCAAAAGATCCGAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RETICULATA-like

Protein Analysis

385

Amino Acids

41.19

Weight (kDa)

9.06

Isoelectric Point (pI)

41.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RETICULATA-like PF11891 157 - 334 4.5e-67 Protein RETICULATA-related
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 981
AccB1I GGYRCC 3 cut(s) 385, 561, 981
AccBSI CCGCTC 3 cut(s) 472, 559, 653
AclWI GGATC 2 cut(s) 171, 1138
AcoI YGGCCR 1 cut(s) 615
AcsI RAATTY 2 cut(s) 571, 1134
AcyI GRCGYC 1 cut(s) 562
AfaI GTAC 2 cut(s) 847, 983
AfiI CCNNNNNNNGG 3 cut(s) 218, 221, 1107
AflIII ACRYGT 1 cut(s) 1086
AgsI TTSAA 4 cut(s) 466, 1007, 1022, 1139
AhlI ACTAGT 1 cut(s) 935
AjnI CCWGG 2 cut(s) 306, 738
AloI GAACNNNNNNTCC 2 cut(s) 476, 508
AluBI AGCT 9 cut(s) 99, 418, 586, 626, 746, 806, 884, 944, 1045
AluI AGCT 9 cut(s) 99, 418, 586, 626, 746, 806, 884, 944, 1045
Alw21I GWGCWC 1 cut(s) 588
Alw26I GTCTC 1 cut(s) 350
AlwI GGATC 2 cut(s) 171, 1138
AoxI GGCC 3 cut(s) 303, 615, 694
ApeKI GCWGC 3 cut(s) 5, 545, 1030
ApoI RAATTY 2 cut(s) 571, 1134
AseI ATTAAT 1 cut(s) 870
Asp700I GAANNNNTTC 1 cut(s) 407
Asp718I GGTACC 1 cut(s) 981
AspLEI GCGC 3 cut(s) 564, 692, 788
AspS9I GGNCC 2 cut(s) 280, 304
AsuC2I CCSGG 1 cut(s) 390
AsuII TTCGAA 1 cut(s) 1075
AvaII GGWCC 1 cut(s) 280
BaeI ACNNNNGTAYC 2 cut(s) 837, 870
BalI TGGCCA 1 cut(s) 617
BanI GGYRCC 3 cut(s) 385, 561, 981
BanII GRGCYC 2 cut(s) 213, 588
Bbv12I GWGCWC 1 cut(s) 588
BbvCI CCTCAGC 1 cut(s) 321
BbvI GCAGC 2 cut(s) 532, 1017
BccI CCATC 7 cut(s) 26, 119, 223, 260, 452, 607, 626
BceAI ACGGC 3 cut(s) 217, 250, 681
BciT130I CCWGG 2 cut(s) 308, 740
BcnI CCSGG 1 cut(s) 390
BcoDI GTCTC 1 cut(s) 350
BcuI ACTAGT 1 cut(s) 935
BfaI CTAG 2 cut(s) 936, 969
BfmI CTRYAG 3 cut(s) 6, 369, 546
BfoI RGCGCY 1 cut(s) 565
BglI GCCNNNNNGGC 1 cut(s) 311
Bme1390I CCNGG 3 cut(s) 308, 390, 740
Bme18I GGWCC 1 cut(s) 280
BmgT120I GGNCC 2 cut(s) 280, 304
BmiI GGNNCC 5 cut(s) 387, 563, 757, 983, 1099
BmrFI CCNGG 3 cut(s) 308, 390, 740
BmsI GCATC 2 cut(s) 519, 842
BpmI CTGGAG 1 cut(s) 440
Bpu10I CCTNAGC 1 cut(s) 321
Bpu14I TTCGAA 1 cut(s) 1075
BpuMI CCSGG 1 cut(s) 390
Bsa29I ATCGAT 1 cut(s) 888
BsaBI GATNNNNATC 1 cut(s) 617
BsaHI GRCGYC 1 cut(s) 562
BsaJI CCNNGG 2 cut(s) 306, 389
BsaXI ACNNNNNCTCC 2 cut(s) 124, 154
Bsc4I CCNNNNNNNGG 3 cut(s) 218, 221, 1107
Bse1I ACTGG 2 cut(s) 832, 1084
Bse3DI GCAATG 1 cut(s) 321
Bse8I GATNNNNATC 1 cut(s) 617
BseBI CCWGG 2 cut(s) 308, 740
BseCI ATCGAT 1 cut(s) 888
BseDI CCNNGG 2 cut(s) 306, 389
BseGI GGATG 2 cut(s) 610, 922
BseJI GATNNNNATC 1 cut(s) 617
BseLI CCNNNNNNNGG 3 cut(s) 218, 221, 1107
BseMI GCAATG 1 cut(s) 321
BseMII CTCAG 2 cut(s) 312, 1043
BseNI ACTGG 2 cut(s) 832, 1084
BseXI GCAGC 2 cut(s) 532, 1017
BseYI CCCAGC 1 cut(s) 326
BsgI GTGCAG 1 cut(s) 1016
BshFI GGCC 3 cut(s) 305, 617, 696
BshNI GGYRCC 3 cut(s) 385, 561, 981
BshVI ATCGAT 1 cut(s) 888
BsiHKAI GWGCWC 1 cut(s) 588
BsiSI CCGG 2 cut(s) 389, 996
BslFI GGGAC 1 cut(s) 838
BslI CCNNNNNNNGG 3 cut(s) 218, 221, 1107
BsmAI GTCTC 1 cut(s) 350
BsmFI GGGAC 1 cut(s) 838
BsnI GGCC 3 cut(s) 305, 617, 696
Bsp119I TTCGAA 1 cut(s) 1075
Bsp1286I GDGCHC 2 cut(s) 213, 588
Bsp143I GATC 3 cut(s) 176, 286, 1143
BspANI GGCC 3 cut(s) 305, 617, 696
BspCNI CTCAG 2 cut(s) 313, 1044
BspDI ATCGAT 1 cut(s) 888
BspLI GGNNCC 5 cut(s) 387, 563, 757, 983, 1099
BspMAI CTGCAG 2 cut(s) 10, 550
BspPI GGATC 2 cut(s) 171, 1138
BspT104I TTCGAA 1 cut(s) 1075
BspT107I GGYRCC 3 cut(s) 385, 561, 981
BsrBI CCGCTC 3 cut(s) 472, 559, 653
BsrDI GCAATG 1 cut(s) 321
BsrI ACTGG 2 cut(s) 832, 1084
BssECI CCNNGG 2 cut(s) 306, 389
BssMI GATC 3 cut(s) 176, 286, 1143
BssNI GRCGYC 1 cut(s) 562
Bst2UI CCWGG 2 cut(s) 308, 740
Bst4CI ACNGT 2 cut(s) 263, 661
Bst6I CTCTTC 1 cut(s) 398
BstACI GRCGYC 1 cut(s) 562
BstAPI GCANNNNNTGC 1 cut(s) 516
BstBI TTCGAA 1 cut(s) 1075
BstC8I GCNNGC 2 cut(s) 480, 694
BstDEI CTNAG 4 cut(s) 321, 705, 977, 1052
BstF5I GGATG 2 cut(s) 610, 922
BstH2I RGCGCY 1 cut(s) 565
BstHHI GCGC 3 cut(s) 564, 692, 788
BstKTI GATC 3 cut(s) 179, 289, 1146
BstMAI GTCTC 1 cut(s) 350
BstMBI GATC 3 cut(s) 176, 286, 1143
BstNI CCWGG 2 cut(s) 308, 740
BstSCI CCNGG 3 cut(s) 306, 388, 738
BstSFI CTRYAG 3 cut(s) 6, 369, 546
BstV1I GCAGC 2 cut(s) 532, 1017
BstX2I RGATCY 1 cut(s) 1143
BstYI RGATCY 1 cut(s) 1143
Bsu15I ATCGAT 1 cut(s) 888
BsuRI GGCC 3 cut(s) 305, 617, 696
BsuTUI ATCGAT 1 cut(s) 888
BtsCI GGATG 2 cut(s) 610, 922
Cac8I GCNNGC 2 cut(s) 480, 694
CfoI GCGC 3 cut(s) 564, 692, 788
Cfr13I GGNCC 2 cut(s) 280, 304
ClaI ATCGAT 1 cut(s) 888
Csp6I GTAC 2 cut(s) 846, 982
CviQI GTAC 2 cut(s) 846, 982
DdeI CTNAG 4 cut(s) 321, 705, 977, 1052
DinI GGCGCC 1 cut(s) 563
DpnI GATC 3 cut(s) 178, 288, 1145
DpnII GATC 3 cut(s) 176, 286, 1143
EaeI YGGCCR 1 cut(s) 615
Eam1104I CTCTTC 1 cut(s) 398
EarI CTCTTC 1 cut(s) 398
EciI GGCGGA 3 cut(s) 237, 252, 314
Ecl136II GAGCTC 1 cut(s) 586
Eco24I GRGCYC 2 cut(s) 213, 588
Eco47I GGWCC 1 cut(s) 280
Eco53kI GAGCTC 1 cut(s) 586
EcoICRI GAGCTC 1 cut(s) 586
EcoO109I RGGNCCY 1 cut(s) 304
EcoRI GAATTC 1 cut(s) 1134
EcoRII CCWGG 2 cut(s) 306, 738
EcoT38I GRGCYC 2 cut(s) 213, 588
EgeI GGCGCC 1 cut(s) 563
EheI GGCGCC 1 cut(s) 563
FalI AAGNNNNNCTT 2 cut(s) 395, 427
FaqI GGGAC 1 cut(s) 838
FauI CCCGC 6 cut(s) 80, 128, 173, 370, 375, 658
FokI GGATG 2 cut(s) 617, 929
FriOI GRGCYC 2 cut(s) 213, 588
FspBI CTAG 2 cut(s) 936, 969
GlaI GCGC 3 cut(s) 563, 691, 787
GsaI CCCAGC 1 cut(s) 330
GsuI CTGGAG 1 cut(s) 440
HaeII RGCGCY 1 cut(s) 565
HaeIII GGCC 3 cut(s) 305, 617, 696
HapII CCGG 2 cut(s) 389, 996
HhaI GCGC 3 cut(s) 564, 692, 788
Hin1I GRCGYC 1 cut(s) 562
Hin6I GCGC 3 cut(s) 562, 690, 786
HinP1I GCGC 3 cut(s) 562, 690, 786
HincII GTYRAC 1 cut(s) 933
HindII GTYRAC 1 cut(s) 933
HindIII AAGCTT 2 cut(s) 97, 1043
HinfI GANTC 1 cut(s) 343
HpaII CCGG 2 cut(s) 389, 996
Hpy166II GTNNAC 2 cut(s) 230, 933
Hpy188I TCNGA 2 cut(s) 340, 1148
Hpy188III TCNNGA 6 cut(s) 349, 496, 725, 753, 823, 1019
Hpy8I GTNNAC 2 cut(s) 230, 933
Hpy99I CGWCG 1 cut(s) 340
HpyAV CCTTC 3 cut(s) 66, 346, 1025
HpyCH4III ACNGT 2 cut(s) 263, 661
HpyCH4IV ACGT 2 cut(s) 1088, 1123
HpyCH4V TGCA 8 cut(s) 8, 548, 818, 833, 875, 1033, 1058, 1121
HpyF3I CTNAG 4 cut(s) 321, 705, 977, 1052
HpySE526I ACGT 2 cut(s) 1088, 1123
Hsp92I GRCGYC 1 cut(s) 562
HspAI GCGC 3 cut(s) 562, 690, 786
KasI GGCGCC 1 cut(s) 561
KpnI GGTACC 1 cut(s) 985
Kzo9I GATC 3 cut(s) 176, 286, 1143
LmnI GCTCC 4 cut(s) 469, 556, 658, 803
Lsp1109I GCAGC 2 cut(s) 532, 1017
LweI GCATC 2 cut(s) 519, 842
MaeI CTAG 2 cut(s) 936, 969
MaeII ACGT 2 cut(s) 1088, 1123
MaeIII GTNAC 2 cut(s) 10, 853
MalI GATC 3 cut(s) 178, 288, 1145
MbiI CCGCTC 3 cut(s) 472, 559, 653
MboI GATC 3 cut(s) 176, 286, 1143
MboII GAAGA 2 cut(s) 415, 703
MflI RGATCY 1 cut(s) 1143
MhlI GDGCHC 2 cut(s) 213, 588
MlsI TGGCCA 1 cut(s) 617
MluCI AATT 5 cut(s) 47, 551, 571, 1002, 1134
MluNI TGGCCA 1 cut(s) 617
Mly113I GGCGCC 1 cut(s) 562
MlyI GAGTC 1 cut(s) 352
MmeI TCCRAC 4 cut(s) 48, 175, 318, 1106
Mox20I TGGCCA 1 cut(s) 617
MroXI GAANNNNTTC 1 cut(s) 407
MscI TGGCCA 1 cut(s) 617
MseI TTAA 1 cut(s) 870
Msp20I TGGCCA 1 cut(s) 617
MspI CCGG 2 cut(s) 389, 996
MspR9I CCNGG 3 cut(s) 308, 390, 740
MvaI CCWGG 2 cut(s) 308, 740
NarI GGCGCC 1 cut(s) 562
NciI CCSGG 1 cut(s) 390
NdeII GATC 3 cut(s) 176, 286, 1143
NlaIV GGNNCC 5 cut(s) 387, 563, 757, 983, 1099
NmeAIII GCCGAG 1 cut(s) 590
NmuCI GTSAC 1 cut(s) 10
NspV TTCGAA 1 cut(s) 1075
PcsI WCGNNNNNNNCGW 1 cut(s) 519
PdmI GAANNNNTTC 1 cut(s) 407
PleI GAGTC 1 cut(s) 351
PluTI GGCGCC 1 cut(s) 565
PpsI GAGTC 1 cut(s) 351
PshBI ATTAAT 1 cut(s) 870
Psp124BI GAGCTC 1 cut(s) 588
Psp6I CCWGG 2 cut(s) 306, 738
PspFI CCCAGC 1 cut(s) 326
PspGI CCWGG 2 cut(s) 306, 738
PspN4I GGNNCC 5 cut(s) 387, 563, 757, 983, 1099
PspPI GGNCC 2 cut(s) 280, 304
PstI CTGCAG 2 cut(s) 10, 550
PsuI RGATCY 1 cut(s) 1143
RsaI GTAC 2 cut(s) 847, 983
RsaNI GTAC 2 cut(s) 846, 982
SacI GAGCTC 1 cut(s) 588
SaqAI TTAA 1 cut(s) 870
Sau3AI GATC 3 cut(s) 176, 286, 1143
Sau96I GGNCC 2 cut(s) 280, 304
SchI GAGTC 1 cut(s) 352
ScrFI CCNGG 3 cut(s) 308, 390, 740
SduI GDGCHC 2 cut(s) 213, 588
SfaNI GCATC 2 cut(s) 519, 842
SfcI CTRYAG 3 cut(s) 6, 369, 546
SfoI GGCGCC 1 cut(s) 563
SfuI TTCGAA 1 cut(s) 1075
SinI GGWCC 1 cut(s) 280
SpeI ACTAGT 1 cut(s) 935
Sse9I AATT 5 cut(s) 47, 551, 571, 1002, 1134
SspDI GGCGCC 1 cut(s) 561
SspMI CTAG 2 cut(s) 936, 969
SstI GAGCTC 1 cut(s) 588
StyD4I CCNGG 3 cut(s) 306, 388, 738
TaaI ACNGT 2 cut(s) 263, 661
TaiI ACGT 2 cut(s) 1091, 1126
TaqI TCGA 7 cut(s) 289, 513, 588, 670, 888, 1075, 1113
TaqII GACCGA 1 cut(s) 297
TasI AATT 5 cut(s) 47, 551, 571, 1002, 1134
TauI GCSGC 8 cut(s) 191, 194, 197, 314, 365, 368, 522, 562
Tru1I TTAA 1 cut(s) 870
Tru9I TTAA 1 cut(s) 870
TseFI GTSAC 1 cut(s) 10
TseI GCWGC 3 cut(s) 5, 545, 1030
Tsp45I GTSAC 1 cut(s) 10
VpaK11BI GGWCC 1 cut(s) 280
VspI ATTAAT 1 cut(s) 870
XapI RAATTY 2 cut(s) 571, 1134
XmnI GAANNNNTTC 1 cut(s) 407
XspI CTAG 2 cut(s) 936, 969
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.