MD03G1172700.v1.1

Protein RETICULATA-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002218
Physical Location & Seq
Reverse (-)
23557535 .. 23561887
4353 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1172700.v1.1.491

Sequence Viewer

Length: 1161 bp
ATGGCGATCGCCTTCTGCTTCCTTTCCTCCTCCGCCGCCGCCGCCTCCTCTTCCTCCATCTCCAAACTCAATCCCCACCGCCCATCTCTCTCCCCCTCCGCCTCCTCGCCCCTCCTCCACCTCCGCCTCACGGCCACCCCTCCCTCCCTCACCCACCGCCACCGCCAGTTCCGCCGCATCCTCTTCTCAGGAGGTGGCGGCGGCGGCGACGGGGGGATTAACGGACGTGACTCCTCAGGAGGTGGCGGCGGCGGAGATGAGGGTGACAGCGACGGAGGGTCGGACAATGCCGGCGGAAAGAACAGGGGGGAGGCGTTGATGGTGTTGGCGGAGTCTAAGCGGGGGTTGGAGAGCTTGCCCAAGGACTTGGCGGCTGCGATCCAGGCGGGTCGGATTCCCGGGTCGGTTGTGGGACGGTACTTTGAGCTAGAGAAGTCTGGTCTGTTCAGGTGGCTGATGCAATTCGCTGGGTTTAGAGAGAGGTTGCTCGCTGATGATCTTTTCTTTGCTAAGGTTTTCATGGAATGCGGCGTTGGTCTCTTCACCAAGACTGCTGCAGAGTACGAGCGGCGGAGAGAGAACTTTTTCAATGAGCTGGAAGTTGTTTTTGCAGATGTGGTAATGGCCATAATTGCAGATTTCATGCTTGTTTATCTTCCTGCCCCAACCGTTTCTCTTCGACCTCCACTTGGGGTCAATGCAGGAGCAATCACTAGGTTTTTCCATGGCTGCCCCGAAAATGCCTTCCAGATCGCCATGTCGGGAACATCATACTCATTATTACAGAGGATTGGTGGAGTAGTGAGAAATGGGGCCAAGCTCTTTGCTGTCGGTACTGCTTCATCCCTGGTTGGCACAGCTGTGACAAATGCCTTGATCAACGCAAGGAAGGCTGTTGATAAGGACAAAGCCGGTGAACTTGAGAATGTGCCGATATTATCCACTAGCGTTGCATATGGTGTCTACATGGCAGTTTCTAGCAATCTCAGGTACCAAGTACTGGCTGGTGTTATTGAACAAAGGTGTTTGGAACCGCTACTCCATCAAAACAAGCTCATGCTAAGCGCAATTTGCTTTGCCATTCGGACTGGAAACACATTCTTGGGTTCATTATTGTGGGTGGACTATGCTCGTTTAATAGGAATCCAGAAGGCTCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RETICULATA-like

Protein Analysis

387

Amino Acids

41.1

Weight (kDa)

9.2

Isoelectric Point (pI)

39.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RETICULATA-like PF11891 160 - 337 7.3e-64 Protein RETICULATA-related
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 990
AccB1I GGYRCC 1 cut(s) 990
AccB7I CCANNNNNTGG 1 cut(s) 1000
AccBSI CCGCTC 1 cut(s) 568
AccI GTMKAC 1 cut(s) 963
AclWI GGATC 1 cut(s) 373
AcoI YGGCCR 2 cut(s) 132, 624
AfaI GTAC 5 cut(s) 419, 563, 835, 992, 999
AfiI CCNNNNNNNGG 3 cut(s) 130, 689, 1000
AgsI TTSAA 2 cut(s) 589, 1016
AhdI GACNNNNNGTC 1 cut(s) 277
AjiI CACGTC 1 cut(s) 227
AjnI CCWGG 2 cut(s) 381, 846
AjuI GAANNNNNNNTTGG 2 cut(s) 516, 548
AleI CACNNNNGTG 1 cut(s) 860
AloI GAACNNNNNNTCC 2 cut(s) 1023, 1055
AluBI AGCT 6 cut(s) 354, 427, 595, 820, 860, 1054
AluI AGCT 6 cut(s) 354, 427, 595, 820, 860, 1054
Alw26I GTCTC 1 cut(s) 542
AlwI GGATC 1 cut(s) 373
Ama87I CYCGRG 1 cut(s) 398
AoxI GGCC 3 cut(s) 132, 624, 813
ApeKI GCWGC 3 cut(s) 374, 554, 729
AsiSI GCGATCGC 1 cut(s) 9
Asp700I GAANNNNTTC 1 cut(s) 584
Asp718I GGTACC 1 cut(s) 990
AspLEI GCGC 1 cut(s) 1067
AspS9I GGNCC 1 cut(s) 813
AsuC2I CCSGG 2 cut(s) 399, 400
AsuHPI GGTGA 4 cut(s) 142, 275, 535, 926
AvaI CYCGRG 1 cut(s) 398
AxyI CCTNAGG 1 cut(s) 235
BalI TGGCCA 1 cut(s) 626
BanI GGYRCC 1 cut(s) 990
BbvI GCAGC 3 cut(s) 361, 541, 716
BccI CCATC 4 cut(s) 65, 91, 313, 1050
BceAI ACGGC 1 cut(s) 147
BciT130I CCWGG 2 cut(s) 383, 848
BclI TGATCA 1 cut(s) 876
BcnI CCSGG 2 cut(s) 399, 400
BcoDI GTCTC 1 cut(s) 542
BfaI CTAG 4 cut(s) 428, 714, 945, 978
BfmI CTRYAG 1 cut(s) 555
BlpI GCTNAGC 1 cut(s) 1061
BmcAI AGTACT 1 cut(s) 999
Bme1390I CCNGG 4 cut(s) 383, 399, 400, 848
BmeRI GACNNNNNGTC 1 cut(s) 277
BmeT110I CYCGRG 1 cut(s) 398
BmgBI CACGTC 1 cut(s) 227
BmgT120I GGNCC 1 cut(s) 813
BmiI GGNNCC 3 cut(s) 814, 992, 1032
BmrFI CCNGG 4 cut(s) 383, 399, 400, 848
BmsI GCATC 2 cut(s) 186, 447
Bpu10I CCTNAGC 1 cut(s) 510
Bpu1102I GCTNAGC 1 cut(s) 1061
BpuEI CTTGAG 1 cut(s) 941
BpuMI CCSGG 2 cut(s) 399, 400
BsaI GGTCTC 1 cut(s) 542
BsaJI CCNNGG 4 cut(s) 360, 398, 724, 846
BsaXI ACNNNNNCTCC 2 cut(s) 1023, 1053
Bsc4I CCNNNNNNNGG 3 cut(s) 130, 689, 1000
Bse118I RCCGGY 2 cut(s) 290, 911
Bse1I ACTGG 3 cut(s) 166, 1005, 1093
Bse21I CCTNAGG 1 cut(s) 235
BseBI CCWGG 2 cut(s) 383, 848
BseDI CCNNGG 4 cut(s) 360, 398, 724, 846
BseGI GGATG 2 cut(s) 177, 842
BseLI CCNNNNNNNGG 3 cut(s) 130, 689, 1000
BseMII CTCAG 3 cut(s) 201, 249, 1000
BseNI ACTGG 3 cut(s) 166, 1005, 1093
BseRI GAGGAG 5 cut(s) 19, 37, 94, 104, 223
BseXI GCAGC 3 cut(s) 361, 541, 716
BseYI CCCAGC 1 cut(s) 467
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 3 cut(s) 134, 626, 815
BshNI GGYRCC 1 cut(s) 990
BsiEI CGRYCG 1 cut(s) 9
BsiHKCI CYCGRG 1 cut(s) 398
BsiSI CCGG 3 cut(s) 291, 399, 912
BslFI GGGAC 1 cut(s) 426
BslI CCNNNNNNNGG 3 cut(s) 130, 689, 1000
BsmAI GTCTC 1 cut(s) 542
BsmFI GGGAC 1 cut(s) 426
BsmI GAATGC 1 cut(s) 530
BsnI GGCC 3 cut(s) 134, 626, 815
Bso31I GGTCTC 1 cut(s) 542
BsoBI CYCGRG 1 cut(s) 398
Bsp143I GATC 5 cut(s) 6, 378, 496, 750, 876
Bsp1720I GCTNAGC 1 cut(s) 1061
Bsp19I CCATGG 1 cut(s) 724
BspANI GGCC 3 cut(s) 134, 626, 815
BspCNI CTCAG 3 cut(s) 200, 248, 999
BspLI GGNNCC 3 cut(s) 814, 992, 1032
BspMAI CTGCAG 1 cut(s) 559
BspPI GGATC 1 cut(s) 373
BspT107I GGYRCC 1 cut(s) 990
BspTNI GGTCTC 1 cut(s) 542
BsrBI CCGCTC 1 cut(s) 568
BsrFI RCCGGY 2 cut(s) 290, 911
BsrI ACTGG 3 cut(s) 166, 1005, 1093
BssAI RCCGGY 2 cut(s) 290, 911
BssECI CCNNGG 4 cut(s) 360, 398, 724, 846
BssMI GATC 5 cut(s) 6, 378, 496, 750, 876
BssT1I CCWWGG 2 cut(s) 360, 724
Bst2UI CCWGG 2 cut(s) 383, 848
Bst4CI ACNGT 2 cut(s) 417, 670
Bst6I CTCTTC 4 cut(s) 55, 188, 545, 681
BstC8I GCNNGC 3 cut(s) 292, 356, 489
BstDEI CTNAG 6 cut(s) 187, 235, 336, 510, 986, 1061
BstDSI CCRYGG 1 cut(s) 724
BstF5I GGATG 2 cut(s) 177, 842
BstHHI GCGC 1 cut(s) 1067
BstKTI GATC 5 cut(s) 9, 381, 499, 753, 879
BstMAI GTCTC 1 cut(s) 542
BstMBI GATC 5 cut(s) 6, 378, 496, 750, 876
BstMCI CGRYCG 1 cut(s) 9
BstMWI GCNNNNNNNGC 7 cut(s) 41, 171, 204, 383, 632, 890, 1071
BstNI CCWGG 2 cut(s) 383, 848
BstSCI CCNGG 4 cut(s) 381, 397, 398, 846
BstSFI CTRYAG 1 cut(s) 555
BstV1I GCAGC 3 cut(s) 361, 541, 716
BstXI CCANNNNNNTGG 1 cut(s) 367
Bsu36I CCTNAGG 1 cut(s) 235
BsuRI GGCC 3 cut(s) 134, 626, 815
BtgI CCRYGG 1 cut(s) 724
BtrI CACGTC 1 cut(s) 227
BtsCI GGATG 2 cut(s) 177, 842
Cac8I GCNNGC 3 cut(s) 292, 356, 489
CfoI GCGC 1 cut(s) 1067
Cfr10I RCCGGY 2 cut(s) 290, 911
Cfr13I GGNCC 1 cut(s) 813
Cfr9I CCCGGG 1 cut(s) 398
Csp6I GTAC 5 cut(s) 418, 562, 834, 991, 998
CspCI CAANNNNNGTGG 2 cut(s) 931, 966
CviAII CATG 6 cut(s) 520, 643, 725, 757, 967, 1057
CviQI GTAC 5 cut(s) 418, 562, 834, 991, 998
DdeI CTNAG 6 cut(s) 187, 235, 336, 510, 986, 1061
DpnI GATC 5 cut(s) 8, 380, 498, 752, 878
DpnII GATC 5 cut(s) 6, 378, 496, 750, 876
DriI GACNNNNNGTC 1 cut(s) 277
EaeI YGGCCR 2 cut(s) 132, 624
Eam1104I CTCTTC 4 cut(s) 55, 188, 545, 681
Eam1105I GACNNNNNGTC 1 cut(s) 277
EarI CTCTTC 4 cut(s) 55, 188, 545, 681
EciI GGCGGA 8 cut(s) 22, 88, 113, 161, 267, 309, 344, 586
Eco130I CCWWGG 2 cut(s) 360, 724
Eco31I GGTCTC 1 cut(s) 542
Eco81I CCTNAGG 1 cut(s) 235
Eco88I CYCGRG 1 cut(s) 398
EcoRII CCWGG 2 cut(s) 381, 846
EcoT14I CCWWGG 2 cut(s) 360, 724
ErhI CCWWGG 2 cut(s) 360, 724
FaeI CATG 6 cut(s) 523, 646, 728, 760, 970, 1060
FaqI GGGAC 1 cut(s) 426
FatI CATG 6 cut(s) 519, 642, 724, 756, 966, 1056
FauI CCCGC 2 cut(s) 333, 379
FauNDI CATATG 1 cut(s) 955
FbaI TGATCA 1 cut(s) 876
FblI GTMKAC 1 cut(s) 963
FokI GGATG 2 cut(s) 164, 829
FspBI CTAG 4 cut(s) 428, 714, 945, 978
GlaI GCGC 1 cut(s) 1066
GsaI CCCAGC 1 cut(s) 471
HaeIII GGCC 3 cut(s) 134, 626, 815
HapII CCGG 3 cut(s) 291, 399, 912
HhaI GCGC 1 cut(s) 1067
Hin1II CATG 6 cut(s) 523, 646, 728, 760, 970, 1060
Hin6I GCGC 1 cut(s) 1065
HinP1I GCGC 1 cut(s) 1065
HinfI GANTC 4 cut(s) 230, 332, 394, 1143
HpaII CCGG 3 cut(s) 291, 399, 912
HphI GGTGA 4 cut(s) 142, 275, 535, 926
Hpy166II GTNNAC 3 cut(s) 917, 964, 1123
Hpy188I TCNGA 3 cut(s) 283, 393, 1086
Hpy188III TCNNGA 5 cut(s) 189, 237, 748, 762, 1147
Hpy8I GTNNAC 3 cut(s) 917, 964, 1123
Hpy99I CGWCG 2 cut(s) 212, 275
HpyAV CCTTC 4 cut(s) 22, 754, 883, 1144
HpyCH4III ACNGT 2 cut(s) 417, 670
HpyCH4IV ACGT 1 cut(s) 226
HpyCH4V TGCA 6 cut(s) 460, 557, 611, 635, 701, 953
HpyF10VI GCNNNNNNNGC 7 cut(s) 41, 171, 204, 383, 632, 890, 1071
HpyF3I CTNAG 6 cut(s) 187, 235, 336, 510, 986, 1061
HpySE526I ACGT 1 cut(s) 226
Hsp92II CATG 6 cut(s) 523, 646, 728, 760, 970, 1060
HspAI GCGC 1 cut(s) 1065
KpnI GGTACC 1 cut(s) 994
KroI GCCGGC 1 cut(s) 290
KroNI GCCGGC 1 cut(s) 292
Ksp22I TGATCA 1 cut(s) 876
Kzo9I GATC 5 cut(s) 6, 378, 496, 750, 876
LmnI GCTCC 1 cut(s) 704
Lsp1109I GCAGC 3 cut(s) 361, 541, 716
LweI GCATC 2 cut(s) 186, 447
MaeI CTAG 4 cut(s) 428, 714, 945, 978
MaeII ACGT 1 cut(s) 226
MaeIII GTNAC 3 cut(s) 227, 263, 862
MalI GATC 5 cut(s) 8, 380, 498, 752, 878
MbiI CCGCTC 1 cut(s) 568
MboI GATC 5 cut(s) 6, 378, 496, 750, 876
MboII GAAGA 5 cut(s) 42, 175, 532, 647, 668
MlsI TGGCCA 1 cut(s) 626
MluCI AATT 3 cut(s) 461, 630, 1068
MluNI TGGCCA 1 cut(s) 626
MlyI GAGTC 2 cut(s) 224, 341
MmeI TCCRAC 3 cut(s) 261, 327, 371
Mox20I TGGCCA 1 cut(s) 626
MroNI GCCGGC 1 cut(s) 290
MroXI GAANNNNTTC 1 cut(s) 584
MscI TGGCCA 1 cut(s) 626
MseI TTAA 2 cut(s) 219, 1136
MslI CAYNNNNRTG 2 cut(s) 860, 1114
Msp20I TGGCCA 1 cut(s) 626
MspA1I CMGCKG 1 cut(s) 860
MspI CCGG 3 cut(s) 291, 399, 912
MspR9I CCNGG 4 cut(s) 383, 399, 400, 848
Mva1269I GAATGC 1 cut(s) 530
MvaI CCWGG 2 cut(s) 383, 848
MwoI GCNNNNNNNGC 7 cut(s) 41, 171, 204, 383, 632, 890, 1071
NaeI GCCGGC 1 cut(s) 292
NciI CCSGG 2 cut(s) 399, 400
NcoI CCATGG 1 cut(s) 724
NdeI CATATG 1 cut(s) 955
NdeII GATC 5 cut(s) 6, 378, 496, 750, 876
NgoMIV GCCGGC 1 cut(s) 290
NlaIII CATG 6 cut(s) 523, 646, 728, 760, 970, 1060
NlaIV GGNNCC 3 cut(s) 814, 992, 1032
NmuCI GTSAC 3 cut(s) 227, 263, 862
OliI CACNNNNGTG 1 cut(s) 860
PctI GAATGC 1 cut(s) 530
PdiI GCCGGC 1 cut(s) 292
PdmI GAANNNNTTC 1 cut(s) 584
PfeI GAWTC 2 cut(s) 394, 1143
PflMI CCANNNNNTGG 1 cut(s) 1000
Ple19I CGATCG 1 cut(s) 9
PleI GAGTC 2 cut(s) 224, 340
PpsI GAGTC 2 cut(s) 224, 340
Psp6I CCWGG 2 cut(s) 381, 846
PspFI CCCAGC 1 cut(s) 467
PspGI CCWGG 2 cut(s) 381, 846
PspN4I GGNNCC 3 cut(s) 814, 992, 1032
PspPI GGNCC 1 cut(s) 813
PstI CTGCAG 1 cut(s) 559
PvuI CGATCG 1 cut(s) 9
PvuII CAGCTG 1 cut(s) 860
RgaI GCGATCGC 1 cut(s) 9
RsaI GTAC 5 cut(s) 419, 563, 835, 992, 999
RsaNI GTAC 5 cut(s) 418, 562, 834, 991, 998
RseI CAYNNNNRTG 2 cut(s) 860, 1114
SaqAI TTAA 2 cut(s) 219, 1136
Sau3AI GATC 5 cut(s) 6, 378, 496, 750, 876
Sau96I GGNCC 1 cut(s) 813
ScaI AGTACT 1 cut(s) 999
SchI GAGTC 2 cut(s) 224, 341
ScrFI CCNGG 4 cut(s) 383, 399, 400, 848
SfaAI GCGATCGC 1 cut(s) 9
SfaNI GCATC 2 cut(s) 186, 447
SfcI CTRYAG 1 cut(s) 555
SgfI GCGATCGC 1 cut(s) 9
SmaI CCCGGG 1 cut(s) 400
SmiMI CAYNNNNRTG 2 cut(s) 860, 1114
SmlI CTYRAG 1 cut(s) 920
SmoI CTYRAG 1 cut(s) 920
Sse9I AATT 3 cut(s) 461, 630, 1068
SspMI CTAG 4 cut(s) 428, 714, 945, 978
StyD4I CCNGG 4 cut(s) 381, 397, 398, 846
StyI CCWWGG 2 cut(s) 360, 724
TaaI ACNGT 2 cut(s) 417, 670
TaiI ACGT 1 cut(s) 229
TaqI TCGA 1 cut(s) 679
TasI AATT 3 cut(s) 461, 630, 1068
TatI WGTACW 1 cut(s) 997
TfiI GAWTC 2 cut(s) 394, 1143
Tru1I TTAA 2 cut(s) 219, 1136
Tru9I TTAA 2 cut(s) 219, 1136
TseFI GTSAC 3 cut(s) 227, 263, 862
TseI GCWGC 3 cut(s) 374, 554, 729
Tsp45I GTSAC 3 cut(s) 227, 263, 862
TspDTI ATGAA 4 cut(s) 508, 631, 831, 1098
TspGWI ACGGA 2 cut(s) 237, 288
TspMI CCCGGG 1 cut(s) 398
Van91I CCANNNNNTGG 1 cut(s) 1000
XcmI CCANNNNNNNNNTGG 1 cut(s) 1001
XmaI CCCGGG 1 cut(s) 398
XmiI GTMKAC 1 cut(s) 963
XmnI GAANNNNTTC 1 cut(s) 584
XspI CTAG 4 cut(s) 428, 714, 945, 978
ZrmI AGTACT 1 cut(s) 999
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.