MD07G1022200.v1.1

Protein RETICULATA-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0002218
Physical Location & Seq
Forward (+)
1865531 .. 1870519
4989 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1022200.v1.1.491

Sequence Viewer

Length: 1167 bp
ATGGCGGCAGTCACCACCATCTCCAACTTCGCCACCTTTCACTCTCAGTTTCTCCCTTCACCAACCAACCCCCACCATTTCTCTTTATCCCCAAAAAACTTCACTCTCACCATCGTCGCCCGCACTAACATCACCGCAACTCCCTCCGTCCAACTCACCGCCTCTCGCTTCACCCGATCCCACCTCGCCTCCGCCGCATACGGCGGTGAACCCCCCGCAGGAGGAGGCGGAGACGGCGGAAGCGGAGGTTATGATAACAATGACAGTGATGGGAGTGGTGATGGTGATGAGGACCGGGATCGGAACAAGGCGGAGGCCCTGGCGGCAATGGCGGAGGCGGGGAGGTCGTCGGAGAGTCTGCCGAAGGACTTAGCGGCGGCCATAAAGGCCGGGCGGGTGCCGGGCTTGATTGTGAAGAGGTTCTTTGAGCTGGAGAAGTCGGCGGTGTTCAGATGGTTGCTTGGGTTTGATGGGTTCAAGGAGCGGTTGCTTGCGGACGACTTGTTCTTGACCAAAGTTGGCATCGAGTGCGGCGTTGGAATATTCACTAAGACTGCTGCAGAATTGGAGCGACGCCGAGAAAATTTTACCAAAGAGCTGGATTTTGTTTTTGCTGATGTGGTGATGGCCATCATAGCTGATTTTATGCTTGTCTGGCTTCCCGCTCCTACTGTTTCGCTTCGACCACCTCTTGCTGTCAGCGCAGGCCGTATTGCTAAGTTCTTCTATGGGTGTCCGGATAATGCTTTCCAGGTAGCTTTGTCTGGAACATCTTACTCACTCTTACAGAGAATAGGTGCAATAGTGAGAAATGGAGCTAAACTATTTGCAGTTGGGACTGGTGCATCTCTGGTTGGTACGGGTGTGACAAATGCATTGATTAATGCACGAAAAGCTCTCGATAAATCCTTTGCTGGTGAAGCTGAGGATGTTCCCATATTATCAACTAGTGTAGCCTATGGAGTTTATATGGCAGTTTCTAGCAATCTAAGGTACCAAGTTCTCGCGGGAGTAATTGAACAGCGACTTCTGGAACCCTTGCTGCACCAACACAAGCTTATACTGAGTGCAATTTGCTTTGCTGTTCGAACTGGCAACACGTTCTTGGGTTCCTTGATGTGGGTCGATTATGCACGTTGGATTGGAATTCAAAAGATCCGAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RETICULATA-like

Protein Analysis

389

Amino Acids

41.63

Weight (kDa)

8.35

Isoelectric Point (pI)

37.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RETICULATA-like PF11891 161 - 338 4.9e-67 Protein RETICULATA-related
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 993
AccB1I GGYRCC 2 cut(s) 397, 993
AccBSI CCGCTC 2 cut(s) 484, 665
AccII CGCG 1 cut(s) 1007
AccIII TCCGGA 1 cut(s) 736
AclWI GGATC 3 cut(s) 171, 306, 1150
AcoI YGGCCR 2 cut(s) 378, 627
AcsI RAATTY 2 cut(s) 583, 1146
AcyI GRCGYC 1 cut(s) 574
AfaI GTAC 2 cut(s) 859, 995
AfiI CCNNNNNNNGG 3 cut(s) 218, 221, 1119
AflIII ACRYGT 1 cut(s) 1098
AgsI TTSAA 3 cut(s) 478, 1019, 1151
AhlI ACTAGT 1 cut(s) 947
AjnI CCWGG 2 cut(s) 318, 750
AloI GAACNNNNNNTCC 2 cut(s) 488, 520
AluBI AGCT 8 cut(s) 430, 598, 638, 758, 818, 896, 923, 1057
AluI AGCT 8 cut(s) 430, 598, 638, 758, 818, 896, 923, 1057
Alw26I GTCTC 1 cut(s) 225
AlwI GGATC 3 cut(s) 171, 306, 1150
Aor13HI TCCGGA 1 cut(s) 736
AoxI GGCC 5 cut(s) 315, 378, 387, 627, 706
ApeKI GCWGC 2 cut(s) 557, 1042
ApoI RAATTY 2 cut(s) 583, 1146
AseI ATTAAT 1 cut(s) 882
Asp700I GAANNNNTTC 1 cut(s) 419
Asp718I GGTACC 1 cut(s) 993
AspLEI GCGC 1 cut(s) 704
AspS9I GGNCC 2 cut(s) 292, 316
AsuC2I CCSGG 3 cut(s) 296, 391, 402
AsuII TTCGAA 1 cut(s) 1087
AvaII GGWCC 1 cut(s) 292
BaeI ACNNNNGTAYC 2 cut(s) 849, 882
BalI TGGCCA 1 cut(s) 629
BanI GGYRCC 2 cut(s) 397, 993
BbvCI CCTCAGC 1 cut(s) 924
BbvI GCAGC 2 cut(s) 544, 1029
BccI CCATC 8 cut(s) 26, 119, 263, 275, 447, 464, 619, 638
BceAI ACGGC 3 cut(s) 217, 250, 693
BciT130I CCWGG 2 cut(s) 320, 752
BcnI CCSGG 3 cut(s) 296, 391, 402
BcoDI GTCTC 1 cut(s) 225
BcuI ACTAGT 1 cut(s) 947
BfaI CTAG 2 cut(s) 948, 981
BfmI CTRYAG 1 cut(s) 558
BglI GCCNNNNNGGC 2 cut(s) 323, 386
BisI GCNGC 8 cut(s) 6, 195, 324, 375, 378, 532, 558, 1043
BlsI GCNGC 8 cut(s) 7, 196, 325, 376, 379, 533, 559, 1044
Bme1390I CCNGG 5 cut(s) 296, 320, 391, 402, 752
Bme18I GGWCC 1 cut(s) 292
BmgT120I GGNCC 2 cut(s) 292, 316
BmiI GGNNCC 4 cut(s) 399, 995, 1035, 1111
BmrFI CCNGG 5 cut(s) 296, 320, 391, 402, 752
BmsI GCATC 2 cut(s) 531, 854
BpmI CTGGAG 1 cut(s) 452
Bpu10I CCTNAGC 1 cut(s) 924
Bpu14I TTCGAA 1 cut(s) 1087
BpuMI CCSGG 3 cut(s) 296, 391, 402
BsaBI GATNNNNATC 1 cut(s) 629
BsaHI GRCGYC 1 cut(s) 574
BsaJI CCNNGG 1 cut(s) 318
BsaWI WCCGGW 1 cut(s) 736
BsaXI ACNNNNNCTCC 4 cut(s) 124, 154, 173, 203
Bsc4I CCNNNNNNNGG 3 cut(s) 218, 221, 1119
Bse1I ACTGG 2 cut(s) 844, 1096
Bse3DI GCAATG 1 cut(s) 333
Bse8I GATNNNNATC 1 cut(s) 629
BseAI TCCGGA 1 cut(s) 736
BseBI CCWGG 2 cut(s) 320, 752
BseDI CCNNGG 1 cut(s) 318
BseGI GGATG 1 cut(s) 934
BseJI GATNNNNATC 1 cut(s) 629
BseLI CCNNNNNNNGG 3 cut(s) 218, 221, 1119
BseMI GCAATG 1 cut(s) 333
BseMII CTCAG 3 cut(s) 59, 915, 1055
BseNI ACTGG 2 cut(s) 844, 1096
BseRI GAGGAG 1 cut(s) 237
BseXI GCAGC 2 cut(s) 544, 1029
BsgI GTGCAG 1 cut(s) 1028
Bsh1236I CGCG 1 cut(s) 1007
BshFI GGCC 5 cut(s) 317, 380, 389, 629, 708
BshNI GGYRCC 2 cut(s) 397, 993
BsiSI CCGG 4 cut(s) 295, 390, 401, 737
BslFI GGGAC 1 cut(s) 850
BslI CCNNNNNNNGG 3 cut(s) 218, 221, 1119
BsmAI GTCTC 1 cut(s) 225
BsmBI CGTCTC 1 cut(s) 225
BsmFI GGGAC 1 cut(s) 850
BsnI GGCC 5 cut(s) 317, 380, 389, 629, 708
Bsp119I TTCGAA 1 cut(s) 1087
Bsp13I TCCGGA 1 cut(s) 736
Bsp143I GATC 3 cut(s) 176, 298, 1155
BspANI GGCC 5 cut(s) 317, 380, 389, 629, 708
BspCNI CTCAG 3 cut(s) 58, 916, 1056
BspEI TCCGGA 1 cut(s) 736
BspFNI CGCG 1 cut(s) 1007
BspLI GGNNCC 4 cut(s) 399, 995, 1035, 1111
BspMAI CTGCAG 1 cut(s) 562
BspPI GGATC 3 cut(s) 171, 306, 1150
BspT104I TTCGAA 1 cut(s) 1087
BspT107I GGYRCC 2 cut(s) 397, 993
BsrBI CCGCTC 2 cut(s) 484, 665
BsrDI GCAATG 1 cut(s) 333
BsrI ACTGG 2 cut(s) 844, 1096
BssECI CCNNGG 1 cut(s) 318
BssMI GATC 3 cut(s) 176, 298, 1155
BssNI GRCGYC 1 cut(s) 574
Bst2UI CCWGG 2 cut(s) 320, 752
Bst4CI ACNGT 2 cut(s) 266, 673
Bst6I CTCTTC 1 cut(s) 410
BstACI GRCGYC 1 cut(s) 574
BstAPI GCANNNNNTGC 1 cut(s) 528
BstBI TTCGAA 1 cut(s) 1087
BstC8I GCNNGC 3 cut(s) 121, 492, 706
BstDEI CTNAG 7 cut(s) 45, 370, 549, 717, 924, 989, 1064
BstF5I GGATG 1 cut(s) 934
BstFNI CGCG 1 cut(s) 1007
BstHHI GCGC 1 cut(s) 704
BstKTI GATC 3 cut(s) 179, 301, 1158
BstMAI GTCTC 1 cut(s) 225
BstMBI GATC 3 cut(s) 176, 298, 1155
BstNI CCWGG 2 cut(s) 320, 752
BstSCI CCNGG 5 cut(s) 294, 318, 389, 400, 750
BstSFI CTRYAG 1 cut(s) 558
BstUI CGCG 1 cut(s) 1007
BstV1I GCAGC 2 cut(s) 544, 1029
BstX2I RGATCY 1 cut(s) 1155
BstXI CCANNNNNNTGG 1 cut(s) 598
BstYI RGATCY 1 cut(s) 1155
BsuRI GGCC 5 cut(s) 317, 380, 389, 629, 708
BtsCI GGATG 1 cut(s) 934
BtsIMutI CAGTG 1 cut(s) 271
Cac8I GCNNGC 3 cut(s) 121, 492, 706
CfoI GCGC 1 cut(s) 704
Cfr13I GGNCC 2 cut(s) 292, 316
CseI GACGC 1 cut(s) 582
Csp6I GTAC 2 cut(s) 858, 994
CviQI GTAC 2 cut(s) 858, 994
DdeI CTNAG 7 cut(s) 45, 370, 549, 717, 924, 989, 1064
DpnI GATC 3 cut(s) 178, 300, 1157
DpnII GATC 3 cut(s) 176, 298, 1155
EaeI YGGCCR 2 cut(s) 378, 627
Eam1104I CTCTTC 1 cut(s) 410
EarI CTCTTC 1 cut(s) 410
EciI GGCGGA 5 cut(s) 181, 243, 252, 326, 347
Eco47I GGWCC 1 cut(s) 292
EcoO109I RGGNCCY 1 cut(s) 316
EcoRI GAATTC 1 cut(s) 1146
EcoRII CCWGG 2 cut(s) 318, 750
EcoT22I ATGCAT 1 cut(s) 877
Esp3I CGTCTC 1 cut(s) 225
FalI AAGNNNNNCTT 2 cut(s) 407, 439
FaqI GGGAC 1 cut(s) 850
FauI CCCGC 6 cut(s) 128, 223, 331, 387, 670, 1000
Fnu4HI GCNGC 8 cut(s) 6, 195, 324, 375, 378, 532, 558, 1043
FokI GGATG 1 cut(s) 941
Fsp4HI GCNGC 8 cut(s) 6, 195, 324, 375, 378, 532, 558, 1043
FspBI CTAG 2 cut(s) 948, 981
GlaI GCGC 1 cut(s) 703
GluI GCNGC 8 cut(s) 6, 195, 324, 375, 378, 532, 558, 1043
GsuI CTGGAG 1 cut(s) 452
HaeIII GGCC 5 cut(s) 317, 380, 389, 629, 708
HapII CCGG 4 cut(s) 295, 390, 401, 737
HgaI GACGC 1 cut(s) 582
HhaI GCGC 1 cut(s) 704
Hin1I GRCGYC 1 cut(s) 574
Hin6I GCGC 1 cut(s) 702
HinP1I GCGC 1 cut(s) 702
HindIII AAGCTT 1 cut(s) 1055
HinfI GANTC 1 cut(s) 355
HpaII CCGG 4 cut(s) 295, 390, 401, 737
Hpy166II GTNNAC 1 cut(s) 209
Hpy188I TCNGA 4 cut(s) 303, 352, 452, 1160
Hpy188III TCNNGA 5 cut(s) 508, 737, 765, 899, 1031
Hpy8I GTNNAC 1 cut(s) 209
Hpy99I CGWCG 3 cut(s) 119, 352, 576
HpyAV CCTTC 2 cut(s) 66, 358
HpyCH4III ACNGT 2 cut(s) 266, 673
HpyCH4IV ACGT 2 cut(s) 1100, 1135
HpyCH4V TGCA 9 cut(s) 560, 800, 830, 845, 875, 887, 1045, 1070, 1133
HpyF3I CTNAG 7 cut(s) 45, 370, 549, 717, 924, 989, 1064
HpySE526I ACGT 2 cut(s) 1100, 1135
Hsp92I GRCGYC 1 cut(s) 574
HspAI GCGC 1 cut(s) 702
Kpn2I TCCGGA 1 cut(s) 736
KpnI GGTACC 1 cut(s) 997
Kzo9I GATC 3 cut(s) 176, 298, 1155
LmnI GCTCC 4 cut(s) 481, 568, 670, 815
Lsp1109I GCAGC 2 cut(s) 544, 1029
LweI GCATC 2 cut(s) 531, 854
MaeI CTAG 2 cut(s) 948, 981
MaeII ACGT 2 cut(s) 1100, 1135
MaeIII GTNAC 2 cut(s) 10, 865
MalI GATC 3 cut(s) 178, 300, 1157
MbiI CCGCTC 2 cut(s) 484, 665
MboI GATC 3 cut(s) 176, 298, 1155
MboII GAAGA 2 cut(s) 427, 715
MflI RGATCY 1 cut(s) 1155
MlsI TGGCCA 1 cut(s) 629
MluCI AATT 5 cut(s) 563, 583, 1014, 1071, 1146
MluNI TGGCCA 1 cut(s) 629
MlyI GAGTC 1 cut(s) 364
MmeI TCCRAC 5 cut(s) 48, 175, 330, 517, 1118
Mox20I TGGCCA 1 cut(s) 629
Mph1103I ATGCAT 1 cut(s) 877
MroI TCCGGA 1 cut(s) 736
MroXI GAANNNNTTC 1 cut(s) 419
MscI TGGCCA 1 cut(s) 629
MseI TTAA 1 cut(s) 882
Msp20I TGGCCA 1 cut(s) 629
MspI CCGG 4 cut(s) 295, 390, 401, 737
MspR9I CCNGG 5 cut(s) 296, 320, 391, 402, 752
MvaI CCWGG 2 cut(s) 320, 752
MvnI CGCG 1 cut(s) 1007
NciI CCSGG 3 cut(s) 296, 391, 402
NdeII GATC 3 cut(s) 176, 298, 1155
NlaIV GGNNCC 4 cut(s) 399, 995, 1035, 1111
NmeAIII GCCGAG 1 cut(s) 602
NmuCI GTSAC 2 cut(s) 10, 865
NsiI ATGCAT 1 cut(s) 877
NspV TTCGAA 1 cut(s) 1087
PcsI WCGNNNNNNNCGW 2 cut(s) 172, 531
PdmI GAANNNNTTC 1 cut(s) 419
PkrI GCNGC 8 cut(s) 7, 196, 325, 376, 379, 533, 559, 1044
PleI GAGTC 1 cut(s) 363
PpsI GAGTC 1 cut(s) 363
PshBI ATTAAT 1 cut(s) 882
Psp6I CCWGG 2 cut(s) 318, 750
PspGI CCWGG 2 cut(s) 318, 750
PspN4I GGNNCC 4 cut(s) 399, 995, 1035, 1111
PspPI GGNCC 2 cut(s) 292, 316
PstI CTGCAG 1 cut(s) 562
PsuI RGATCY 1 cut(s) 1155
RsaI GTAC 2 cut(s) 859, 995
RsaNI GTAC 2 cut(s) 858, 994
SaqAI TTAA 1 cut(s) 882
SatI GCNGC 8 cut(s) 6, 195, 324, 375, 378, 532, 558, 1043
Sau3AI GATC 3 cut(s) 176, 298, 1155
Sau96I GGNCC 2 cut(s) 292, 316
SchI GAGTC 1 cut(s) 364
ScrFI CCNGG 5 cut(s) 296, 320, 391, 402, 752
SfaNI GCATC 2 cut(s) 531, 854
SfcI CTRYAG 1 cut(s) 558
SfiI GGCCNNNNNGGCC 1 cut(s) 386
SfuI TTCGAA 1 cut(s) 1087
SinI GGWCC 1 cut(s) 292
SpeI ACTAGT 1 cut(s) 947
Sse9I AATT 5 cut(s) 563, 583, 1014, 1071, 1146
SspI AATATT 1 cut(s) 543
SspMI CTAG 2 cut(s) 948, 981
StyD4I CCNGG 5 cut(s) 294, 318, 389, 400, 750
TaaI ACNGT 2 cut(s) 266, 673
TaiI ACGT 2 cut(s) 1103, 1138
TaqI TCGA 5 cut(s) 525, 682, 900, 1087, 1125
TasI AATT 5 cut(s) 563, 583, 1014, 1071, 1146
TauI GCSGC 6 cut(s) 8, 197, 326, 377, 380, 534
Tru1I TTAA 1 cut(s) 882
Tru9I TTAA 1 cut(s) 882
TscAI CASTG 1 cut(s) 271
TseFI GTSAC 2 cut(s) 10, 865
TseI GCWGC 2 cut(s) 557, 1042
Tsp45I GTSAC 2 cut(s) 10, 865
TspGWI ACGGA 1 cut(s) 136
TspRI CASTG 1 cut(s) 271
VpaK11BI GGWCC 1 cut(s) 292
VspI ATTAAT 1 cut(s) 882
XapI RAATTY 2 cut(s) 583, 1146
XmnI GAANNNNTTC 1 cut(s) 419
XspI CTAG 2 cut(s) 948, 981
Zsp2I ATGCAT 1 cut(s) 877
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.