Rroxscaffold_4G00330080

Protein RETICULATA-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002218
Physical Location & Seq
Reverse (-)
63449551 .. 63453317
3767 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00330080.1

Sequence Viewer

Length: 1131 bp
ATGGCGGTCACCACCTTGTCCAATTTCTCCACTCTCACCACTCGCACACCCTCTCTCTCTTCTTCTTCTTCACCTTTCCCGCGCACCACAAACTTCACTTTCACTACCCTCACCAAACCTCCCACCTCCCTCGCTCTCGCCACGTCACCGCGCCTCCCCCGCTCCTCTTTCGCCACGTGTTTCTCCTCCGGTGGCCCACCTTCCTCCGGCGGCGGCGGCGGAGGAGGAGATCACGGAAATGGCGGCGGTGACGGAGAGGGAGGGGATGACAGAGAGAGGAACAAAAAGGAGGCGGCGATGGCGATTGCGGAGGCGGGAAGGACGGCGGAGAGTCTGCCGAAGGACTTGGCTGCGGCGGTGGAGGCGGGTCGGGTCCCCGGGTTGATTATGAAGAGGTTCTTTGAGCTGGAGAAGTCGGTGATCTTCCGGTGGCTGCTTCAGTTTGATGGATTCAAGGAGCGGTTGCTGGCCGACGACTTGTTCTTGACCAAAGTAGGCATCGAGTGCGGTGTTGGAATTTTCACCAAGACTGCTGCAGAGTTGGAGCGGCGCAGAGAAAAATTTACCAAGGAGCTGGATTTTGTGTTTGCGGATGTGGTGATGGCCATTATAGCTGATTTTATGCTTGTCTGGCTTCCTGCTCCTACTGTTTCGCTTCGAAAACCTCTTGTTGTTAGTGCTGGGCGTGTTGCTAAGTTCTTCTATGGCTGTCCTGACAATGCATTTCAGGTAGCTCTGGCTGGAACATCTTACTCAGTCTTACAGAGAATAGGCGCAATATTGAGAAATGGAGCAAAACTATTCGTGGTTGGGACCGGTGCATCTCTGATTGGTACTGGTGTAACAAATGCATTGATTGCTGCAAGAAAAGCTTTGGACAAATCCTTTCTTGGTGAAGCGGAAGATGTTCCCATATTATCAACTAGTGCAGCCTATGGGGTCTATATGGCAGTTTCTAGCAATCTAAGGTACCAAGTTCTCGCTGGAATAATCGAACAACGTATTCTAGAACCCTTGCTACACCAACACAAGCTTGCACTGAGTGCAATTTGCTTTGCTGTTCGAACTGGAAACACATTTTTGGGGTCCTTGATGTGGGTTGATTATGCACGCTGGATTGGAATTCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RETICULATA-like

Protein Analysis

376

Amino Acids

40.16

Weight (kDa)

9.24

Isoelectric Point (pI)

39.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RETICULATA-like PF11891 153 - 330 5.1e-66 Protein RETICULATA-related
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 969
AccB1I GGYRCC 1 cut(s) 969
AccBSI CCGCTC 3 cut(s) 162, 460, 547
AccII CGCG 2 cut(s) 82, 151
AcoI YGGCCR 2 cut(s) 468, 603
AcsI RAATTY 3 cut(s) 516, 560, 1122
AcuI CTGAAG 1 cut(s) 422
AcvI CACGTG 1 cut(s) 177
AdeI CACNNNGTG 1 cut(s) 1043
AfaI GTAC 2 cut(s) 835, 971
AfiI CCNNNNNNNGG 2 cut(s) 206, 1095
AflIII ACRYGT 1 cut(s) 176
AgeI ACCGGT 1 cut(s) 815
AgsI TTSAA 2 cut(s) 454, 1127
AhlI ACTAGT 1 cut(s) 923
AjiI CACGTC 1 cut(s) 144
AluBI AGCT 6 cut(s) 406, 574, 614, 734, 872, 1033
AluI AGCT 6 cut(s) 406, 574, 614, 734, 872, 1033
Ama87I CYCGRG 1 cut(s) 377
AoxI GGCC 3 cut(s) 193, 468, 603
ApeKI GCWGC 5 cut(s) 350, 433, 533, 860, 929
ApoI RAATTY 3 cut(s) 516, 560, 1122
AsiGI ACCGGT 1 cut(s) 815
Asp700I GAANNNNTTC 2 cut(s) 395, 906
Asp718I GGTACC 1 cut(s) 969
AspLEI GCGC 4 cut(s) 84, 153, 552, 776
AspS9I GGNCC 4 cut(s) 194, 373, 813, 1086
AsuC2I CCSGG 2 cut(s) 378, 379
AsuHPI GGTGA 9 cut(s) 28, 63, 103, 138, 260, 430, 514, 610, 905
AsuII TTCGAA 2 cut(s) 658, 1063
AvaI CYCGRG 1 cut(s) 377
AvaII GGWCC 3 cut(s) 373, 813, 1086
BaeI ACNNNNGTAYC 2 cut(s) 825, 858
BalI TGGCCA 1 cut(s) 605
BanI GGYRCC 1 cut(s) 969
BbrPI CACGTG 1 cut(s) 177
BbvI GCAGC 5 cut(s) 337, 420, 520, 847, 941
BccI CCATC 3 cut(s) 292, 440, 595
BceAI ACGGC 1 cut(s) 339
BcnI CCSGG 2 cut(s) 378, 379
BcuI ACTAGT 1 cut(s) 923
BfaI CTAG 3 cut(s) 924, 957, 1007
BfmI CTRYAG 1 cut(s) 534
Bme1390I CCNGG 2 cut(s) 378, 379
Bme18I GGWCC 3 cut(s) 373, 813, 1086
BmeT110I CYCGRG 1 cut(s) 377
BmgBI CACGTC 1 cut(s) 144
BmgT120I GGNCC 4 cut(s) 194, 373, 813, 1086
BmiI GGNNCC 5 cut(s) 374, 375, 814, 971, 1087
BmrFI CCNGG 2 cut(s) 378, 379
BmsI GCATC 2 cut(s) 507, 830
BpmI CTGGAG 1 cut(s) 428
Bpu14I TTCGAA 2 cut(s) 658, 1063
BpuMI CCSGG 2 cut(s) 378, 379
BsaAI YACGTR 1 cut(s) 177
BsaJI CCNNGG 3 cut(s) 376, 377, 567
BsaWI WCCGGW 3 cut(s) 188, 426, 815
BsaXI ACNNNNNCTCC 4 cut(s) 103, 133, 138, 168
Bsc4I CCNNNNNNNGG 2 cut(s) 206, 1095
Bse118I RCCGGY 1 cut(s) 815
Bse1I ACTGG 2 cut(s) 841, 1072
BseDI CCNNGG 3 cut(s) 376, 377, 567
BseGI GGATG 2 cut(s) 271, 598
BseLI CCNNNNNNNGG 2 cut(s) 206, 1095
BseMII CTCAG 2 cut(s) 768, 1031
BseNI ACTGG 2 cut(s) 841, 1072
BseRI GAGGAG 4 cut(s) 154, 175, 237, 240
BseXI GCAGC 5 cut(s) 337, 420, 520, 847, 941
BseYI CCCAGC 1 cut(s) 680
BsgI GTGCAG 1 cut(s) 948
Bsh1236I CGCG 2 cut(s) 82, 151
BshFI GGCC 3 cut(s) 195, 470, 605
BshNI GGYRCC 1 cut(s) 969
BshTI ACCGGT 1 cut(s) 815
BsiHKCI CYCGRG 1 cut(s) 377
BsiSI CCGG 5 cut(s) 189, 207, 378, 427, 816
BslFI GGGAC 2 cut(s) 359, 826
BslI CCNNNNNNNGG 2 cut(s) 206, 1095
BsmFI GGGAC 2 cut(s) 359, 826
BsnI GGCC 3 cut(s) 195, 470, 605
BsoBI CYCGRG 1 cut(s) 377
Bsp119I TTCGAA 2 cut(s) 658, 1063
Bsp143I GATC 2 cut(s) 229, 420
BspANI GGCC 3 cut(s) 195, 470, 605
BspCNI CTCAG 2 cut(s) 767, 1032
BspFNI CGCG 2 cut(s) 82, 151
BspLI GGNNCC 5 cut(s) 374, 375, 814, 971, 1087
BspMAI CTGCAG 1 cut(s) 538
BspT104I TTCGAA 2 cut(s) 658, 1063
BspT107I GGYRCC 1 cut(s) 969
BsrBI CCGCTC 3 cut(s) 162, 460, 547
BsrFI RCCGGY 1 cut(s) 815
BsrI ACTGG 2 cut(s) 841, 1072
BssAI RCCGGY 1 cut(s) 815
BssECI CCNNGG 3 cut(s) 376, 377, 567
BssMI GATC 2 cut(s) 229, 420
BssT1I CCWWGG 1 cut(s) 567
Bst4CI ACNGT 1 cut(s) 649
Bst6I CTCTTC 2 cut(s) 64, 386
BstAPI GCANNNNNTGC 3 cut(s) 504, 857, 1043
BstBAI YACGTR 1 cut(s) 177
BstBI TTCGAA 2 cut(s) 658, 1063
BstC8I GCNNGC 3 cut(s) 468, 1035, 1111
BstDEI CTNAG 4 cut(s) 693, 754, 965, 1040
BstEII GGTNACC 1 cut(s) 7
BstF5I GGATG 2 cut(s) 271, 598
BstFNI CGCG 2 cut(s) 82, 151
BstHHI GCGC 4 cut(s) 84, 153, 552, 776
BstKTI GATC 2 cut(s) 232, 423
BstMBI GATC 2 cut(s) 229, 420
BstPI GGTNACC 1 cut(s) 7
BstSCI CCNGG 2 cut(s) 376, 377
BstSFI CTRYAG 1 cut(s) 534
BstUI CGCG 2 cut(s) 82, 151
BstV1I GCAGC 5 cut(s) 337, 420, 520, 847, 941
BstXI CCANNNNNNTGG 1 cut(s) 574
BsuRI GGCC 3 cut(s) 195, 470, 605
BtgZI GCGATG 1 cut(s) 311
BtrI CACGTC 1 cut(s) 144
BtsCI GGATG 2 cut(s) 271, 598
BtsIMutI CAGTG 1 cut(s) 1037
Cac8I GCNNGC 3 cut(s) 468, 1035, 1111
CfoI GCGC 4 cut(s) 84, 153, 552, 776
Cfr10I RCCGGY 1 cut(s) 815
Cfr13I GGNCC 4 cut(s) 194, 373, 813, 1086
Cfr9I CCCGGG 1 cut(s) 377
Csp6I GTAC 2 cut(s) 834, 970
CspAI ACCGGT 1 cut(s) 815
CviQI GTAC 2 cut(s) 834, 970
DdeI CTNAG 4 cut(s) 693, 754, 965, 1040
DpnI GATC 2 cut(s) 231, 422
DpnII GATC 2 cut(s) 229, 420
DraIII CACNNNGTG 1 cut(s) 1043
EaeI YGGCCR 2 cut(s) 468, 603
Eam1104I CTCTTC 2 cut(s) 64, 386
EarI CTCTTC 2 cut(s) 64, 386
EciI GGCGGA 2 cut(s) 234, 341
Eco130I CCWWGG 1 cut(s) 567
Eco47I GGWCC 3 cut(s) 373, 813, 1086
Eco57I CTGAAG 1 cut(s) 422
Eco72I CACGTG 1 cut(s) 177
Eco88I CYCGRG 1 cut(s) 377
Eco91I GGTNACC 1 cut(s) 7
EcoO109I RGGNCCY 2 cut(s) 373, 1086
EcoO65I GGTNACC 1 cut(s) 7
EcoRI GAATTC 1 cut(s) 1122
EcoT14I CCWWGG 1 cut(s) 567
EcoT22I ATGCAT 2 cut(s) 724, 853
ErhI CCWWGG 1 cut(s) 567
FaiI YATR 9 cut(s) 389, 611, 623, 705, 914, 936, 945, 947, 1107
FalI AAGNNNNNCTT 4 cut(s) 383, 415, 856, 888
FaqI GGGAC 2 cut(s) 359, 826
FauI CCCGC 4 cut(s) 87, 167, 307, 358
FokI GGATG 2 cut(s) 278, 605
FspBI CTAG 3 cut(s) 924, 957, 1007
GlaI GCGC 4 cut(s) 83, 152, 551, 775
GsaI CCCAGC 1 cut(s) 684
GsuI CTGGAG 1 cut(s) 428
HaeIII GGCC 3 cut(s) 195, 470, 605
HapII CCGG 5 cut(s) 189, 207, 378, 427, 816
HhaI GCGC 4 cut(s) 84, 153, 552, 776
Hin6I GCGC 4 cut(s) 82, 151, 550, 774
HinP1I GCGC 4 cut(s) 82, 151, 550, 774
HindIII AAGCTT 2 cut(s) 870, 1031
HinfI GANTC 2 cut(s) 331, 450
HpaII CCGG 5 cut(s) 189, 207, 378, 427, 816
HphI GGTGA 9 cut(s) 28, 63, 103, 138, 260, 430, 514, 610, 905
Hpy188I TCNGA 1 cut(s) 828
Hpy188III TCNNGA 3 cut(s) 484, 713, 1007
Hpy99I CGWCG 1 cut(s) 476
HpyAV CCTTC 3 cut(s) 210, 312, 334
HpyCH4III ACNGT 1 cut(s) 649
HpyCH4IV ACGT 3 cut(s) 143, 176, 1000
HpyCH4V TGCA 9 cut(s) 536, 722, 821, 851, 863, 929, 1037, 1046, 1109
HpyF3I CTNAG 4 cut(s) 693, 754, 965, 1040
HpySE526I ACGT 3 cut(s) 143, 176, 1000
HspAI GCGC 4 cut(s) 82, 151, 550, 774
KflI GGGWCCC 1 cut(s) 373
KpnI GGTACC 1 cut(s) 973
Kzo9I GATC 2 cut(s) 229, 420
LmnI GCTCC 6 cut(s) 167, 457, 544, 571, 646, 791
Lsp1109I GCAGC 5 cut(s) 337, 420, 520, 847, 941
LweI GCATC 2 cut(s) 507, 830
MaeI CTAG 3 cut(s) 924, 957, 1007
MaeII ACGT 3 cut(s) 143, 176, 1000
MaeIII GTNAC 4 cut(s) 7, 144, 248, 841
MalI GATC 2 cut(s) 231, 422
MbiI CCGCTC 3 cut(s) 162, 460, 547
MboI GATC 2 cut(s) 229, 420
MboII GAAGA 8 cut(s) 51, 54, 57, 60, 403, 415, 691, 914
MlsI TGGCCA 1 cut(s) 605
MluCI AATT 5 cut(s) 22, 516, 560, 1047, 1122
MluNI TGGCCA 1 cut(s) 605
MlyI GAGTC 1 cut(s) 340
MmeI TCCRAC 2 cut(s) 493, 522
Mox20I TGGCCA 1 cut(s) 605
Mph1103I ATGCAT 2 cut(s) 724, 853
MroXI GAANNNNTTC 2 cut(s) 395, 906
MscI TGGCCA 1 cut(s) 605
MslI CAYNNNNRTG 1 cut(s) 237
Msp20I TGGCCA 1 cut(s) 605
MspI CCGG 5 cut(s) 189, 207, 378, 427, 816
MspR9I CCNGG 2 cut(s) 378, 379
MvnI CGCG 2 cut(s) 82, 151
NciI CCSGG 2 cut(s) 378, 379
NdeII GATC 2 cut(s) 229, 420
NlaIV GGNNCC 5 cut(s) 374, 375, 814, 971, 1087
NmuCI GTSAC 3 cut(s) 7, 144, 248
NsiI ATGCAT 2 cut(s) 724, 853
NspV TTCGAA 2 cut(s) 658, 1063
PdmI GAANNNNTTC 2 cut(s) 395, 906
PfeI GAWTC 1 cut(s) 450
PinAI ACCGGT 1 cut(s) 815
PleI GAGTC 1 cut(s) 339
PmaCI CACGTG 1 cut(s) 177
PmlI CACGTG 1 cut(s) 177
PpsI GAGTC 1 cut(s) 339
Ppu21I YACGTR 1 cut(s) 177
PpuMI RGGWCCY 2 cut(s) 373, 1086
Psp5II RGGWCCY 2 cut(s) 373, 1086
PspCI CACGTG 1 cut(s) 177
PspEI GGTNACC 1 cut(s) 7
PspFI CCCAGC 1 cut(s) 680
PspN4I GGNNCC 5 cut(s) 374, 375, 814, 971, 1087
PspPI GGNCC 4 cut(s) 194, 373, 813, 1086
PspPPI RGGWCCY 2 cut(s) 373, 1086
PsrI GAACNNNNNNTAC 2 cut(s) 1002, 1034
PstI CTGCAG 1 cut(s) 538
RsaI GTAC 2 cut(s) 835, 971
RsaNI GTAC 2 cut(s) 834, 970
RseI CAYNNNNRTG 1 cut(s) 237
Sau3AI GATC 2 cut(s) 229, 420
Sau96I GGNCC 4 cut(s) 194, 373, 813, 1086
SchI GAGTC 1 cut(s) 340
ScrFI CCNGG 2 cut(s) 378, 379
SfaNI GCATC 2 cut(s) 507, 830
SfcI CTRYAG 1 cut(s) 534
SfuI TTCGAA 2 cut(s) 658, 1063
SinI GGWCC 3 cut(s) 373, 813, 1086
SmaI CCCGGG 1 cut(s) 379
SmiMI CAYNNNNRTG 1 cut(s) 237
SpeI ACTAGT 1 cut(s) 923
Sse9I AATT 5 cut(s) 22, 516, 560, 1047, 1122
SspI AATATT 1 cut(s) 780
SspMI CTAG 3 cut(s) 924, 957, 1007
StyD4I CCNGG 2 cut(s) 376, 377
StyI CCWWGG 1 cut(s) 567
TaaI ACNGT 1 cut(s) 649
TaiI ACGT 3 cut(s) 146, 179, 1003
TaqI TCGA 4 cut(s) 501, 658, 993, 1063
TasI AATT 5 cut(s) 22, 516, 560, 1047, 1122
TauI GCSGC 7 cut(s) 213, 216, 219, 246, 296, 356, 550
TfiI GAWTC 1 cut(s) 450
TscAI CASTG 1 cut(s) 1044
TseFI GTSAC 3 cut(s) 7, 144, 248
TseI GCWGC 5 cut(s) 350, 433, 533, 860, 929
Tsp45I GTSAC 3 cut(s) 7, 144, 248
TspDTI ATGAA 1 cut(s) 404
TspGWI ACGGA 2 cut(s) 249, 267
TspMI CCCGGG 1 cut(s) 377
TspRI CASTG 1 cut(s) 1044
VpaK11BI GGWCC 3 cut(s) 373, 813, 1086
XapI RAATTY 3 cut(s) 516, 560, 1122
XbaI TCTAGA 1 cut(s) 1006
XcmI CCANNNNNNNNNTGG 1 cut(s) 980
XmaI CCCGGG 1 cut(s) 377
XmnI GAANNNNTTC 2 cut(s) 395, 906
XspI CTAG 3 cut(s) 924, 957, 1007
Zsp2I ATGCAT 2 cut(s) 724, 853
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.