Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1253 |
| AccBSI | CCGCTC | 1 cut(s) | 742 |
| AccI | GTMKAC | 1 cut(s) | 897 |
| AccII | CGCG | 1 cut(s) | 962 |
| AciI | CCGC | 7 cut(s) | 318, 729, 732, 740, 962, 1217, 1340 |
| AclWI | GGATC | 1 cut(s) | 72 |
| AcoI | YGGCCR | 1 cut(s) | 208 |
| AcsI | RAATTY | 4 cut(s) | 568, 788, 871, 1255 |
| AfaI | GTAC | 3 cut(s) | 419, 638, 975 |
| AflII | CTTAAG | 1 cut(s) | 440 |
| AflIII | ACRYGT | 2 cut(s) | 348, 681 |
| AgeI | ACCGGT | 3 cut(s) | 543, 752, 1198 |
| AgsI | TTSAA | 5 cut(s) | 271, 647, 1122, 1315, 1370 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 904 |
| AhlI | ACTAGT | 1 cut(s) | 1240 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1350, 1382 |
| AluBI | AGCT | 6 cut(s) | 130, 152, 223, 475, 562, 799 |
| AluI | AGCT | 6 cut(s) | 130, 152, 223, 475, 562, 799 |
| Alw21I | GWGCWC | 2 cut(s) | 477, 1080 |
| Alw26I | GTCTC | 3 cut(s) | 6, 113, 772 |
| AlwI | GGATC | 1 cut(s) | 72 |
| AoxI | GGCC | 3 cut(s) | 208, 451, 498 |
| ApeKI | GCWGC | 2 cut(s) | 233, 367 |
| ApoI | RAATTY | 4 cut(s) | 568, 788, 871, 1255 |
| AsiGI | ACCGGT | 3 cut(s) | 543, 752, 1198 |
| AspS9I | GGNCC | 6 cut(s) | 146, 451, 498, 663, 750, 966 |
| AsuHPI | GGTGA | 1 cut(s) | 586 |
| AvaII | GGWCC | 4 cut(s) | 146, 663, 750, 966 |
| BalI | TGGCCA | 1 cut(s) | 210 |
| BanII | GRGCYC | 1 cut(s) | 477 |
| BauI | CACGAG | 1 cut(s) | 775 |
| Bbv12I | GWGCWC | 2 cut(s) | 477, 1080 |
| BbvI | GCAGC | 2 cut(s) | 220, 379 |
| BccI | CCATC | 5 cut(s) | 245, 251, 407, 674, 934 |
| BclI | TGATCA | 1 cut(s) | 1029 |
| BcoDI | GTCTC | 3 cut(s) | 6, 113, 772 |
| BcuI | ACTAGT | 1 cut(s) | 1240 |
| BfaI | CTAG | 4 cut(s) | 116, 162, 224, 1241 |
| BfmI | CTRYAG | 1 cut(s) | 274 |
| BfrI | CTTAAG | 1 cut(s) | 440 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 216 |
| BisI | GCNGC | 3 cut(s) | 234, 368, 730 |
| BlsI | GCNGC | 3 cut(s) | 235, 369, 731 |
| Bme18I | GGWCC | 4 cut(s) | 146, 663, 750, 966 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 904 |
| BmgT120I | GGNCC | 6 cut(s) | 146, 451, 498, 663, 750, 966 |
| BmiI | GGNNCC | 5 cut(s) | 548, 664, 945, 967, 1360 |
| BmsI | GCATC | 3 cut(s) | 871, 1253, 1391 |
| BpmI | CTGGAG | 1 cut(s) | 612 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1431 |
| BsaJI | CCNNGG | 3 cut(s) | 214, 762, 1362 |
| BsaWI | WCCGGW | 5 cut(s) | 479, 543, 752, 1198, 1208 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 824, 854 |
| Bse118I | RCCGGY | 3 cut(s) | 543, 752, 1198 |
| Bse1I | ACTGG | 1 cut(s) | 871 |
| Bse3DI | GCAATG | 2 cut(s) | 1009, 1032 |
| BseDI | CCNNGG | 3 cut(s) | 214, 762, 1362 |
| BseGI | GGATG | 5 cut(s) | 855, 886, 1116, 1268, 1314 |
| BseMI | GCAATG | 2 cut(s) | 1009, 1032 |
| BseMII | CTCAG | 1 cut(s) | 209 |
| BseNI | ACTGG | 1 cut(s) | 871 |
| BseRI | GAGGAG | 2 cut(s) | 95, 398 |
| BseXI | GCAGC | 2 cut(s) | 220, 379 |
| Bsh1236I | CGCG | 1 cut(s) | 962 |
| BshFI | GGCC | 3 cut(s) | 210, 453, 500 |
| BshTI | ACCGGT | 3 cut(s) | 543, 752, 1198 |
| BsiHKAI | GWGCWC | 2 cut(s) | 477, 1080 |
| BsiSI | CCGG | 6 cut(s) | 480, 544, 753, 813, 1199, 1209 |
| BsiWI | CGTACG | 1 cut(s) | 973 |
| BslFI | GGGAC | 2 cut(s) | 676, 979 |
| BsmAI | GTCTC | 3 cut(s) | 6, 113, 772 |
| BsmBI | CGTCTC | 2 cut(s) | 6, 113 |
| BsmFI | GGGAC | 2 cut(s) | 676, 979 |
| BsnI | GGCC | 3 cut(s) | 210, 453, 500 |
| Bsp1286I | GDGCHC | 2 cut(s) | 477, 1080 |
| Bsp1407I | TGTACA | 1 cut(s) | 417 |
| Bsp143I | GATC | 5 cut(s) | 64, 180, 371, 1029, 1347 |
| Bsp19I | CCATGG | 2 cut(s) | 762, 1362 |
| BspACI | CCGC | 7 cut(s) | 318, 729, 732, 740, 962, 1217, 1340 |
| BspANI | GGCC | 3 cut(s) | 210, 453, 500 |
| BspCNI | CTCAG | 1 cut(s) | 208 |
| BspFNI | CGCG | 1 cut(s) | 962 |
| BspLI | GGNNCC | 5 cut(s) | 548, 664, 945, 967, 1360 |
| BspPI | GGATC | 1 cut(s) | 72 |
| BspTI | CTTAAG | 1 cut(s) | 440 |
| BsrBI | CCGCTC | 1 cut(s) | 742 |
| BsrDI | GCAATG | 2 cut(s) | 1009, 1032 |
| BsrFI | RCCGGY | 3 cut(s) | 543, 752, 1198 |
| BsrGI | TGTACA | 1 cut(s) | 417 |
| BsrI | ACTGG | 1 cut(s) | 871 |
| BssAI | RCCGGY | 3 cut(s) | 543, 752, 1198 |
| BssECI | CCNNGG | 3 cut(s) | 214, 762, 1362 |
| BssMI | GATC | 5 cut(s) | 64, 180, 371, 1029, 1347 |
| BssSI | CACGAG | 1 cut(s) | 775 |
| BssT1I | CCWWGG | 2 cut(s) | 762, 1362 |
| Bst2BI | CACGAG | 1 cut(s) | 775 |
| Bst4CI | ACNGT | 2 cut(s) | 1132, 1190 |
| Bst6I | CTCTTC | 3 cut(s) | 287, 814, 1123 |
| BstAFI | CTTAAG | 1 cut(s) | 440 |
| BstAUI | TGTACA | 1 cut(s) | 417 |
| BstC8I | GCNNGC | 6 cut(s) | 208, 221, 455, 560, 626, 1000 |
| BstDEI | CTNAG | 3 cut(s) | 195, 795, 1431 |
| BstDSI | CCRYGG | 2 cut(s) | 762, 1362 |
| BstF5I | GGATG | 5 cut(s) | 855, 886, 1116, 1268, 1314 |
| BstFNI | CGCG | 1 cut(s) | 962 |
| BstKTI | GATC | 5 cut(s) | 67, 183, 374, 1032, 1350 |
| BstMAI | GTCTC | 3 cut(s) | 6, 113, 772 |
| BstMBI | GATC | 5 cut(s) | 64, 180, 371, 1029, 1347 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 216, 332, 959, 1022, 1427 |
| BstNSI | RCATGY | 2 cut(s) | 352, 919 |
| BstSFI | CTRYAG | 1 cut(s) | 274 |
| BstUI | CGCG | 1 cut(s) | 962 |
| BstV1I | GCAGC | 2 cut(s) | 220, 379 |
| BsuRI | GGCC | 3 cut(s) | 210, 453, 500 |
| BtgI | CCRYGG | 2 cut(s) | 762, 1362 |
| BtgZI | GCGATG | 2 cut(s) | 451, 652 |
| BtsCI | GGATG | 5 cut(s) | 855, 886, 1116, 1268, 1314 |
| BtsIMutI | CAGTG | 2 cut(s) | 1044, 1195 |
| Cac8I | GCNNGC | 6 cut(s) | 208, 221, 455, 560, 626, 1000 |
| Cfr10I | RCCGGY | 3 cut(s) | 543, 752, 1198 |
| Cfr13I | GGNCC | 6 cut(s) | 146, 451, 498, 663, 750, 966 |
| Csp6I | GTAC | 3 cut(s) | 418, 637, 974 |
| CspAI | ACCGGT | 3 cut(s) | 543, 752, 1198 |
| CviAII | CATG | 7 cut(s) | 349, 448, 687, 763, 916, 1300, 1363 |
| CviQI | GTAC | 3 cut(s) | 418, 637, 974 |
| DdeI | CTNAG | 3 cut(s) | 195, 795, 1431 |
| DpnI | GATC | 5 cut(s) | 66, 182, 373, 1031, 1349 |
| DpnII | GATC | 5 cut(s) | 64, 180, 371, 1029, 1347 |
| DriI | GACNNNNNGTC | 1 cut(s) | 904 |
| EaeI | YGGCCR | 1 cut(s) | 208 |
| Eam1104I | CTCTTC | 3 cut(s) | 287, 814, 1123 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 904 |
| EarI | CTCTTC | 3 cut(s) | 287, 814, 1123 |
| Ecl136II | GAGCTC | 1 cut(s) | 475 |
| Eco130I | CCWWGG | 2 cut(s) | 762, 1362 |
| Eco24I | GRGCYC | 1 cut(s) | 477 |
| Eco47I | GGWCC | 4 cut(s) | 146, 663, 750, 966 |
| Eco53kI | GAGCTC | 1 cut(s) | 475 |
| EcoICRI | GAGCTC | 1 cut(s) | 475 |
| EcoRI | GAATTC | 1 cut(s) | 788 |
| EcoT14I | CCWWGG | 2 cut(s) | 762, 1362 |
| EcoT22I | ATGCAT | 1 cut(s) | 917 |
| EcoT38I | GRGCYC | 1 cut(s) | 477 |
| ErhI | CCWWGG | 2 cut(s) | 762, 1362 |
| Esp3I | CGTCTC | 2 cut(s) | 6, 113 |
| FaeI | CATG | 7 cut(s) | 352, 451, 690, 766, 919, 1303, 1366 |
| FaqI | GGGAC | 2 cut(s) | 676, 979 |
| FatI | CATG | 7 cut(s) | 348, 447, 686, 762, 915, 1299, 1362 |
| FauI | CCCGC | 2 cut(s) | 955, 1333 |
| FbaI | TGATCA | 1 cut(s) | 1029 |
| FblI | GTMKAC | 1 cut(s) | 897 |
| Fnu4HI | GCNGC | 3 cut(s) | 234, 368, 730 |
| FokI | GGATG | 5 cut(s) | 862, 893, 1123, 1275, 1321 |
| FriOI | GRGCYC | 1 cut(s) | 477 |
| Fsp4HI | GCNGC | 3 cut(s) | 234, 368, 730 |
| FspBI | CTAG | 4 cut(s) | 116, 162, 224, 1241 |
| GluI | GCNGC | 3 cut(s) | 234, 368, 730 |
| GsuI | CTGGAG | 1 cut(s) | 612 |
| HaeIII | GGCC | 3 cut(s) | 210, 453, 500 |
| HapII | CCGG | 6 cut(s) | 480, 544, 753, 813, 1199, 1209 |
| Hin1II | CATG | 7 cut(s) | 352, 451, 690, 766, 919, 1303, 1366 |
| HincII | GTYRAC | 2 cut(s) | 250, 1444 |
| HindII | GTYRAC | 2 cut(s) | 250, 1444 |
| HinfI | GANTC | 9 cut(s) | 49, 165, 430, 436, 654, 767, 899, 1020, 1177 |
| HpaI | GTTAAC | 1 cut(s) | 1444 |
| HpaII | CCGG | 6 cut(s) | 480, 544, 753, 813, 1199, 1209 |
| HphI | GGTGA | 1 cut(s) | 586 |
| Hpy166II | GTNNAC | 5 cut(s) | 250, 610, 637, 898, 1444 |
| Hpy188I | TCNGA | 4 cut(s) | 146, 435, 1355, 1462 |
| Hpy188III | TCNNGA | 4 cut(s) | 169, 591, 644, 775 |
| Hpy8I | GTNNAC | 5 cut(s) | 250, 610, 637, 898, 1444 |
| Hpy99I | CGWCG | 1 cut(s) | 20 |
| HpyAV | CCTTC | 3 cut(s) | 20, 34, 535 |
| HpyCH4III | ACNGT | 2 cut(s) | 1132, 1190 |
| HpyCH4IV | ACGT | 4 cut(s) | 255, 639, 681, 894 |
| HpyCH4V | TGCA | 4 cut(s) | 367, 581, 884, 915 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 216, 332, 959, 1022, 1427 |
| HpyF3I | CTNAG | 3 cut(s) | 195, 795, 1431 |
| HpySE526I | ACGT | 4 cut(s) | 255, 639, 681, 894 |
| Hsp92II | CATG | 7 cut(s) | 352, 451, 690, 766, 919, 1303, 1366 |
| Ksp22I | TGATCA | 1 cut(s) | 1029 |
| KspAI | GTTAAC | 1 cut(s) | 1444 |
| Kzo9I | GATC | 5 cut(s) | 64, 180, 371, 1029, 1347 |
| LmnI | GCTCC | 3 cut(s) | 332, 411, 995 |
| Lsp1109I | GCAGC | 2 cut(s) | 220, 379 |
| LweI | GCATC | 3 cut(s) | 871, 1253, 1391 |
| MaeI | CTAG | 4 cut(s) | 116, 162, 224, 1241 |
| MaeII | ACGT | 4 cut(s) | 255, 639, 681, 894 |
| MaeIII | GTNAC | 3 cut(s) | 166, 597, 1039 |
| MalI | GATC | 5 cut(s) | 66, 182, 373, 1031, 1349 |
| MbiI | CCGCTC | 1 cut(s) | 742 |
| MboI | GATC | 5 cut(s) | 64, 180, 371, 1029, 1347 |
| MboII | GAAGA | 9 cut(s) | 58, 136, 274, 579, 801, 949, 1110, 1172, 1342 |
| MhlI | GDGCHC | 2 cut(s) | 477, 1080 |
| MlsI | TGGCCA | 1 cut(s) | 210 |
| MluNI | TGGCCA | 1 cut(s) | 210 |
| MlyI | GAGTC | 4 cut(s) | 174, 424, 663, 893 |
| MmeI | TCCRAC | 4 cut(s) | 124, 285, 1088, 1219 |
| Mox20I | TGGCCA | 1 cut(s) | 210 |
| Mph1103I | ATGCAT | 1 cut(s) | 917 |
| MscI | TGGCCA | 1 cut(s) | 210 |
| MseI | TTAA | 6 cut(s) | 59, 441, 564, 854, 1052, 1443 |
| MslI | CAYNNNNRTG | 4 cut(s) | 290, 617, 1037, 1073 |
| Msp20I | TGGCCA | 1 cut(s) | 210 |
| MspCI | CTTAAG | 1 cut(s) | 440 |
| MspI | CCGG | 6 cut(s) | 480, 544, 753, 813, 1199, 1209 |
| MvnI | CGCG | 1 cut(s) | 962 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 216, 332, 959, 1022, 1427 |
| NcoI | CCATGG | 2 cut(s) | 762, 1362 |
| NdeII | GATC | 5 cut(s) | 64, 180, 371, 1029, 1347 |
| NlaIII | CATG | 7 cut(s) | 352, 451, 690, 766, 919, 1303, 1366 |
| NlaIV | GGNNCC | 5 cut(s) | 548, 664, 945, 967, 1360 |
| NmeAIII | GCCGAG | 1 cut(s) | 195 |
| NmuCI | GTSAC | 3 cut(s) | 166, 597, 1039 |
| NsiI | ATGCAT | 1 cut(s) | 917 |
| NspI | RCATGY | 2 cut(s) | 352, 919 |
| PciI | ACATGT | 1 cut(s) | 348 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 252 |
| PfeI | GAWTC | 5 cut(s) | 49, 436, 767, 1020, 1177 |
| Pfl23II | CGTACG | 1 cut(s) | 973 |
| PinAI | ACCGGT | 3 cut(s) | 543, 752, 1198 |
| PkrI | GCNGC | 3 cut(s) | 235, 369, 731 |
| PleI | GAGTC | 4 cut(s) | 173, 424, 662, 893 |
| PpsI | GAGTC | 4 cut(s) | 173, 424, 662, 893 |
| PscI | ACATGT | 1 cut(s) | 348 |
| PsiI | TTATAA | 1 cut(s) | 1253 |
| Psp124BI | GAGCTC | 1 cut(s) | 477 |
| PspLI | CGTACG | 1 cut(s) | 973 |
| PspN4I | GGNNCC | 5 cut(s) | 548, 664, 945, 967, 1360 |
| PspPI | GGNCC | 6 cut(s) | 146, 451, 498, 663, 750, 966 |
| RsaI | GTAC | 3 cut(s) | 419, 638, 975 |
| RsaNI | GTAC | 3 cut(s) | 418, 637, 974 |
| RseI | CAYNNNNRTG | 4 cut(s) | 290, 617, 1037, 1073 |
| SacI | GAGCTC | 1 cut(s) | 477 |
| SaqAI | TTAA | 6 cut(s) | 59, 441, 564, 854, 1052, 1443 |
| SatI | GCNGC | 3 cut(s) | 234, 368, 730 |
| Sau3AI | GATC | 5 cut(s) | 64, 180, 371, 1029, 1347 |
| Sau96I | GGNCC | 6 cut(s) | 146, 451, 498, 663, 750, 966 |
| SchI | GAGTC | 4 cut(s) | 174, 424, 663, 893 |
| SduI | GDGCHC | 2 cut(s) | 477, 1080 |
| SfaNI | GCATC | 3 cut(s) | 871, 1253, 1391 |
| SfcI | CTRYAG | 1 cut(s) | 274 |
| SinI | GGWCC | 4 cut(s) | 146, 663, 750, 966 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 290, 617, 1037, 1073 |
| SmlI | CTYRAG | 1 cut(s) | 440 |
| SmoI | CTYRAG | 1 cut(s) | 440 |
| SpeI | ACTAGT | 1 cut(s) | 1240 |
| SsiI | CCGC | 7 cut(s) | 318, 729, 732, 740, 962, 1217, 1340 |
| SspMI | CTAG | 4 cut(s) | 116, 162, 224, 1241 |
| SstI | GAGCTC | 1 cut(s) | 477 |
| StyI | CCWWGG | 2 cut(s) | 762, 1362 |
| TaaI | ACNGT | 2 cut(s) | 1132, 1190 |
| TaiI | ACGT | 4 cut(s) | 258, 642, 684, 897 |
| TaqI | TCGA | 1 cut(s) | 920 |
| TatI | WGTACW | 1 cut(s) | 417 |
| TauI | GCSGC | 1 cut(s) | 732 |
| TfiI | GAWTC | 5 cut(s) | 49, 436, 767, 1020, 1177 |
| Tru1I | TTAA | 6 cut(s) | 59, 441, 564, 854, 1052, 1443 |
| Tru9I | TTAA | 6 cut(s) | 59, 441, 564, 854, 1052, 1443 |
| TscAI | CASTG | 2 cut(s) | 1044, 1195 |
| TseFI | GTSAC | 3 cut(s) | 166, 597, 1039 |
| TseI | GCWGC | 2 cut(s) | 233, 367 |
| Tsp45I | GTSAC | 3 cut(s) | 166, 597, 1039 |
| TspDTI | ATGAA | 4 cut(s) | 274, 436, 1002, 1264 |
| TspGWI | ACGGA | 1 cut(s) | 105 |
| TspRI | CASTG | 2 cut(s) | 1044, 1195 |
| Vha464I | CTTAAG | 1 cut(s) | 440 |
| VpaK11BI | GGWCC | 4 cut(s) | 146, 663, 750, 966 |
| XapI | RAATTY | 4 cut(s) | 568, 788, 871, 1255 |
| XceI | RCATGY | 2 cut(s) | 352, 919 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 976 |
| XmiI | GTMKAC | 1 cut(s) | 897 |
| XspI | CTAG | 4 cut(s) | 116, 162, 224, 1241 |
| Zsp2I | ATGCAT | 1 cut(s) | 917 |